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An. acad. bras. ciênc ; 89(3): 1785-1799, July-Sept. 2017. tab, graf
Article in English | LILACS | ID: biblio-886736


ABSTRACT The effect of the ethanolic extract of propolis (EEP) on Aeromonas hydrophila was analyzed by determination of minimum inhibitory concentration (MIC). Then, the effects of crude propolis powder (CPP) on growth, hemato-immune parameters of the Nile tilapia, as well as its effects on resistance to A. hydrophila challenge were investigated. The CPP (0.5, 1.0, 1.5, 2.0, 2.5 and 3.0%) was added to the diet of 280 Nile tilapia (50.0 ± 5.7 g fish-1). Hemato-immune parameters were analyzed before and after the bacterial challenge. Red blood cell, hematocrit, hemoglobin, mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), and hydrogen peroxide (H2O2) and nitric oxide (NO) were evaluated. The MIC of the EEP was 13% (v/v) with a bactericidal effect after 24 hours. Growth performance was significantly lower for those fish fed diets containing 2.5 and 3% of CPP compared to the control diet. Differences in CPP levels affected fish hemoglobin, neutrophils number and NO following the bacterial challenge. For others parameters no significant differences were observed. Our results show that although propolis has bactericidal properties in vitro, the addition of crude propolis powder to Nile tilapia extruded diets does not necessarily lead to an improvement of fish health.

Animals , Propolis/administration & dosage , Gram-Negative Bacterial Infections/veterinary , Aeromonas hydrophila/drug effects , Cichlids/microbiology , Fish Diseases/diet therapy , Propolis/therapeutic use , Microbial Sensitivity Tests , Gram-Negative Bacterial Infections/diet therapy , Gram-Negative Bacterial Infections/microbiology , Dietary Supplements , Cichlids/blood , Fish Diseases/microbiology , Immunity, Innate
Int. j. morphol ; 35(2): 756-764, June 2017. ilus
Article in English | LILACS | ID: biblio-893051


Methotrexate (MTX) is widely used in the treatment of some forms of cancer but having severe side effects. The present work aimed to investigate the protective role of propolis treatment against alterations induced by MTX on the hepatic and renal tissues. Rabbits were exposed to MTX (0.25 mg/kg), with or without propolis (50 mg/kg) while hepatic and renal biopsies were examined for histological and histochemical abnormalities. Methotrexate induced hydropic degeneration, pyknosis, sinusoidal dilatation and bile duct hyperplasia in the liver together with renal tubular degeneration, glomerular shrinkage and hyaline droplet precipitation. While propolis partially ameliorated some of the morphometric and biochemical alterations, none of the hepatic alterations induced by MTX was protected by propolis treatment. Nevertheless glomerular shrinkage and renal tubule degeneration were partially protected in animals received both MTX plus propolis. It is concluded that propolis treatment has little or no ameliorative effect in protecting the hepatic and renal tissues from MTX toxicity.

El metotrexato (MTX) es ampliamente utilizado en el tratamiento de algunas formas de cáncer, pero tiene efectos secundarios graves. El presente trabajo tuvo como objetivo investigar el papel protector del tratamiento con própoleo frente a las alteraciones inducidas por el MTX en los tejidos hepático y renal. Se expusieron conejos a MTX (0,25 mg / kg), en grupos con y sin propóleo (50 mg / kg), y se realizaron biopsias hepáticas y renales, que fueron examinadas buscando anomalías histológicas e histoquímicas. El metotrexato indujo la degeneración hidrópica, picnosis, dilatación sinusoidal e hiperplasia del conducto biliar en el hígado, junto con la degeneración tubular renal, la contracción glomerular y la precipitación hialina. Mientras que el propóleo parcialmente mejoró algunas de las alteraciones morfométricas y bioquímicas, ninguna de las alteraciones hepáticas inducidas por MTX fue protegido por el tratamiento con propóleo. Sin embargo, la contracción glomerular y la degeneración de los túbulos renales fueron parcialmente protegidos en animales que recibieron MTX más propóleo. Se concluye que el tratamiento con propóleo tiene poco o ningún efecto mejorador en la protección de los tejidos hepáticos y renales sometidos a la toxicidad de MTX.

Animals , Male , Rabbits , Propolis/administration & dosage , Methotrexate/toxicity , Kidney/drug effects , Liver/drug effects , Body Weight , Disease Models, Animal , Kidney/pathology , Liver/pathology
Braz. j. biol ; 75(2): 491-496, 05/2015. tab, graf
Article in English | LILACS | ID: lil-749675


This study evaluated the acute inflammatory response induced by carrageenin in the swim bladder of Nile tilapia supplemented with the mixture of natural extracts of propolis and Aloe barbadensis (1:1) at a concentration of 0.5%, 1% and 2% in diet during 15 days. Thirty-six fish were distributed into four treatments with three replicates: fish supplemented with 0.5% of admix of extracts of propolis and Aloe (1:1) injected with 500 µg carrageenin; fish supplemented with 1% of admix of extracts of propolis and Aloe (1:1) injected with 500 µg carrageenin; fish supplemented with 2% of admix of extracts of propolis and Aloe (1:1), injected with 500 µg carrageenin and unsupplemented fish injected with 500 µg carrageenin. Six hours after injection, samples of blood and exudate from the swim bladder of fish were collected. It was observed an increase in the leukocyte count in the swim bladder exudate of fish supplemented with extracts of propolis and Aloe injected with carrageenin. The most frequent cells were macrophages followed by granular leukocytes, thrombocytes and lymphocytes. Supplementation with propolis and Aloe to 0.5% caused a significant increase in the number of cells on the inflammatory focus mainly macrophages, cells responsible for the phagocytic activity in tissues, agent of innate fish immune response.

Este estudo avaliou a resposta inflamatória aguda induzida por carragenina na bexiga natatóriade tilápia do Nilo suplementada com a mistura dos extratos naturais de própolis e Aloe barbadensis (1:1), nas concentrações de 0,5%, 1% e 2% na dieta durante o período de 15 dias. Trinta e seis peixes foram distribuídos em quatro tratamentos com três repetições: peixes suplementados com 0,5% da mistura dos extratos de própolis e Aloe (1:1) injetados na bexiga natatória com 500 µg de carragenina; peixes suplementados com 1% da mistura dos extratos de própolis e Aloe (1:1) injetados na bexiga natatória com 500 µg de carragenina; peixes suplementados com 2% da mistura dos extratos de própolis e Aloe (1:1) injetados na bexiga natatória com 500 µg de carragenina e peixes não suplementados injetados na bexiga natatória com 500 µg de carragenina. Seis horas após as injeções foram coletadas amostras de sangue e exsudato da bexiga natatória dos peixes. Foi observado aumento na contagem de leucócitos no exsudato da bexiga natatória de peixes suplementados com os extratos de própolis e Aloe injetados com carragenina. As células mais frequentes foram os macrófagos seguidos pelos leucócitos granulares, trombócitos e linfócitos. A suplementação com própolis e Aloe a 0,5% provocou aumento significativo no número de células no foco inflamatório, principalmente dos macrófagos, células responsáveis pela atividade fagocitária nos tecidos, agente da resposta imune inata nos peixes.

Animals , Aloe/chemistry , Cichlids , Dietary Supplements , Inflammation/prevention & control , Propolis/administration & dosage , Urinary Bladder Diseases/veterinary , Acute Disease , Blood Cell Count , Carrageenan , Cichlids/blood , Inflammation/chemically induced , Plant Extracts/administration & dosage , Urinary Bladder Diseases/chemically induced , Urinary Bladder Diseases/prevention & control
Braz. j. biol ; 75(2): 414-422, 05/2015. tab, graf
Article in English | LILACS | ID: lil-749679


Aims The present study verified the effect of propolis alone and its association with swimming in dyslipidemia, left ventricular hypertrophy and atherogenesis of hypercholesterolemic mice. Methods and Results The experiments were performed in LDLr–/– mice, fed with high fat diet for 75 days, and were divided into four experimental groups (n=10): HL, sedentary, subjected to aquatic stress (5 min per day, 5 times per week); NAT submitted to a swimming protocol (1 hour per day, 5 times per week) from the 16th day of the experiment; PRO, sedentary, submitted to aquatic stress and which received oral propolis extract (70 uL/animal/day) from the 16th day of the experiment; HL+NAT+PRO, submitted to swimming and which received propolis as described above. After 75 days, blood was collected for analysis of serum lipids. The ratio between the ventricular weight (mg) and the animal weight (g) was calculated. Histological sections of the heart and aorta were processed immunohistochemically with anti-CD40L antibodies to evaluate the inflammatory process; stained with hematoxylin/eosin and picrosirius red to assess morphological and morphometric alterations. The HL animals showed severe dyslipidemia, atherogenesis and left ventricular hypertrophy, associated with a decrease in serum HDLc levels and subsequent development of cardiovascular inflammatory process, characterized by increased expression of CD40L in the left ventricle and aorta. Swimming and propolis alone and/or associated prevented the LVH, atherogenesis and arterial and ventricular inflammation, decreasing the CD40L expression and increasing the HDLc plasmatic levels. Conclusion Propolis alone or associated with a regular physical activity is beneficial in cardiovascular protection through anti-inflammatory action. .

Objetivos O presente estudo verificou o efeito do própolis associação ou não com a natação na dislipidemia, na hipertrofia ventricular esquerda e aterogênese de camundongos hipercolesterolêmicos. Métodos e Resultados Os experimentos foram realizados em camundongos LDLr–/–, alimentados com dieta hiperlipídica por 75 dias, e divididos em quatro grupos experimentais (n = 10): HL, sedentários, foram submetidos ao estresse aquático (5 min por dia, cinco vezes por semana); NAT foram submetidos a um protocolo de natação (1 hora por dia, cinco vezes por semana) a partir do 16° dia do experimento; PRO, sedentários, submetidos a estresse aquático e que receberam extrato de própolis oral (70 uL / animal / dia) a partir do 16° dia do experimento; HL + NAC + PRO, submetidos a natação e que recebeu a própolis, como descrito acima. Após 75 dias, foi coletado sangue para análise do perfil lipídico. Calculou-se a relação entre o peso ventricular (mg) e o peso do animal (g). Os cortes histológicos do coração e aorta foram processados imunohistoquímicamente com anticorpos anti-CD40L para avaliar o processo inflamatório, corados com hematoxilina / eosina e picrossírius red, para avaliar as alterações morfológicas e morfométricas. Os camundongos HL apresentaram dislipidemia grave, aterogênese e hipertrofia do ventrículo esquerdo, associada a uma diminuição dos níveis plasmáticos de HDLc e o desenvolvimento subsequente do processo inflamatório cardiovasculares, caracterizada pelo aumento da expressão do CD40L no ventrículo esquerdo e na aorta. Natação e a própolis isolado e / ou associados preveniram a HVE, a aterogênese e a inflamação tanto na artéria quanto no ventrículo, diminuindo a expressão de CD40L, aumentando os níveis plasmáticos de HDLc. Conclusão A Própolis isolada ou associada a uma atividade física regular é benéfica na proteção cardiovascular através da ação anti-inflamatória. .

Animals , Male , Mice , Atherosclerosis/prevention & control , Hypercholesterolemia/complications , Hypertrophy, Left Ventricular/prevention & control , Physical Conditioning, Animal , Propolis/administration & dosage , Swimming , Atherosclerosis/etiology , Hypertrophy, Left Ventricular/etiology
Article in Portuguese | LILACS | ID: lil-737694


O presente estudo teve por objetivo verificar o efeito tópico da própolis na proliferação de fibroblastos e a disposição e volume de fibras colágenas presentes durante o processo de reparo tecidual. Foram utilizados ratos wistar, machos, divididos em dois grupos: Grupo Controle (CC) n=16 lesão tratada com creme não-iônico; Grupo Própolis (PP) n=16 lesão tratada com creme não-iônico + Própolis 10%. Nos 4º, 7°, 14° e 21° dias de tratamento foram sacrificados 4 animais de cada grupo em câmara de gás carbônico. O tecido lesionado foi coletado e fixado em formalina a 10% por 48 horas, incluído em álcool a 70%, fixado em parafina e depositado em lâminas para análise histológica. Os resultados demonstraram um aumento no número de fibroblastos e também maior e melhor disposição de fibras colágenas no grupo PP em relação ao grupo CC. Assim, as evidências obtidas no estudo mostraram que o efeito da própolis na aceleração do processo de reparo tecidual não é somente por sua ação antiinflamatória, conforme diversos estudos demonstram, mas também por sua ação direta sobre a proliferação de fibroblastos, acelerando a reversão de fibrócito para fibroblasto, e, consequentemente favorecendo a síntese e deposição de fibras colágenas, melhorando o reparo tecidual e reduzindo o tempo de cicatrização...

This study aimed to verify the effect of topical propolis on fibroblast proliferation and disposicao and volume of collagen fibers present in the tissue repair process. We used male Wistar rats were divided into two groups: control (CC) n = 16 lesions treated with non-ionic cream; Propolis Group (PP) n = 16 lesion treated with non-ionic cream + 10% propolis. At 4, 7, 14 and 21 days of treatment where sacrificed four animals from each group in a carbon dioxide chamber. The injured tissue was collected and fixed in 10% formalin for 48 hours, then in 70% alcohol, embedded in paraffin and placed on slides for histological analysis. The results showed an increase in the number of fibroblasts and also bigger and better arrangement of collagen fibers in PP group than in CC group. Thus, the evidence obtained in the study showed that the effect of propolis to speed up tissuerepair process is not only for its anti-inflammatory action, as several studies show, but also by its direct action on the proliferation of fibroblasts, accelerating the rate at which fibrocytes revert to fibroblasts, and collagen fiber arrangement, improving tissue repair and reducing the healing time...

Animals , Male , Rats , Fibroblasts , Skin/injuries , Propolis/pharmacology , Rats , Skin/anatomy & histology , Cell Proliferation , Propolis/administration & dosage
Int. j. morphol ; 32(2): 522-530, jun. 2014. ilus
Article in English | LILACS | ID: lil-714303


The aim of this study was to assess the effect of oral administration of Hydroalcoholic Extract of Green Propolis (HEGP) on dermal carcinogenesis in rodent model. For the biological assay, we used 36 mice, assigned into 6 groups (n=6): CTR (treated with 100 mg/kg HEGP and no tumor induction), TUM (treated with water and tumor induction), GP10 (treated with 10 mg/kg HEGP and tumor induction), GP50 (treated with 50 mg/kg HEGP and tumor induction) and GP100 (treated with 100 mg/kg HEGP and tumor induction). Cancer induction was performed in the back of the mice by topical application of DMBA. After 16 weeks, mice were euthanized and their backs were submitted to post-mortem histological analysis. The mean number of lesions developed in TUM (4.14±0.89) was significantly higher than in GP10 (2.05±1.02), GP50 (1.8±1.92) and GP100 (2.5±1.73) (p<0.05). The tumors formed in HEGP-treated groups were histologically more differentiated, but only in PV100 in situ lesions were evidenced. Infiltration of anatomical noble structures was less frequent in HEGP-treated groups (p<0.05). Our data suggest that oral administration of HEGP provided partial inhibition of DMBA-induced dermal carcinogenesis, as well as appeared to modulate the differentiation and infiltrative potential of the carcinomas in rodent model.

El objetivo de este estudio fue evaluar el efecto de la administración oral de extracto hidroalcohólico del propóleos verde (HEGP) sobre la carcinogénesis dérmica en modelo de roedores. Para el ensayo biológico, se utilizaron 36 ratones asignados en 6 grupos (n = 6): CTR (tratado con 100 mg/kg HEGP y sin inducción de tumores), TUM (tratada con agua e inducción de tumores), GP10 (tratado con 10 mg/kg HEGP e inducción de tumores), GP50 (tratado con 50 mg/kg HEGP e inducción de tumores) y GP100 (tratado con 100 mg/kg HEGP e inducción de tumores). La inducción de cáncer se llevó a cabo en la región dorsal de los ratones por aplicación tópica de DMBA. Después de 16 semanas, los ratones fueron sacrificados y sus dorsos fueron sometidos a análisis histológico post-mortem. El número medio de lesiones desarrolladas en TUM (4,14±0,89) fue significativamente mayor que GP10 (2,05±1,02), GP50 (1,8±1,92) y gp100 (2,5±1,73) (p<0,05). Los tumores formados en grupos tratados con HEGP fueron histológicamente más diferenciados, pero sólo en PV100 las lesiones in situ fueron manifiestas. La infiltración de las estructuras anatómicas blanco fue menos frecuente en los grupos tratados con HEGP (p<0,05). Nuestros datos sugieren que la administración oral de HEGP proporciona una inhibición parcial de la carcinogénesis dérmica inducida por DMBA, así como pareció modular la diferenciación y potencial infiltrante de los carcinomas en el modelo animal.

Animals , Mice , Propolis/administration & dosage , Skin Neoplasms/prevention & control , Carcinogenesis/drug effects , Propolis/pharmacology , Propolis/chemistry , Skin Neoplasms/chemically induced , Flavonoids/analysis , Administration, Oral , Chemoprevention , 9,10-Dimethyl-1,2-benzanthracene , Disease Models, Animal , Alcohols
Arq. ciênc. vet. zool. UNIPAR ; 17(1): 43-48, jan.-mar. 2014. graf
Article in Portuguese | LILACS | ID: lil-758547


Este experimento foi realizado no Laboratório de Sericicultura, no Campus Sede da Universidade Paranaense (UNIPAR) de Umuarama, no período de fevereiro a outubro de 2011, com o objetivo de verificar o efeito da própolis em diferentes dosagens na alimentação durante o desenvolvimento biológico do bicho-da-seda (Bombxy mori L.). O método empregado na parte experimental foi a pulverização do extrato glicólico de própolis, diluído em 500mL de água destilada nas folhas de amoreira, nas seguintes dosagens, água-controle, 25mL, 30mL, 35mL e 40mL compondo os tratamentos: controle, T1 , T2 , T3 e T4 respectivamente. As folhas de amoreira foram fornecidas cinco vezes ao dia, durante o manejo alimentar. Verificou-se, pelos resultados obtidos, que as diferentes dosagens de própolis utilizadas não interferiram no ganho de peso das lagartas, no peso dos casulos verdes, no peso da casca sérica e crisálidas, quando comparado ao tratamento controle, mas quando se compara o Controle e T4 do ensaio da primavera, respectivamente, para os teores de seda bruto e líquido, há resultados significativos. Portanto, verificou-se que o extrato glicólico de própolis, em dosagens de 40mL, pode prejudicar o teor líquido de seda em uma produção de casulos, trazendo resultados pouco apreciados dentro da sericicultura...

This experiment was performed at the Laboratory of Sericulture, at the main campus of University Paranaense (UNIPAR), in the city of Umuarama, from February to October 2011, in order to verify the effect of different doses of propolis in feeding during the biological development of silkworm (Bombyx mori L.). The method used in the experiment was the spraying of propolis glycolic extract dissolved in 500-mL distilled water on the mulberry leaves in the following water- -control dosages: 25mL, 30mL, 35mL and 40mL related to the treatment controls T1, T2, T3 and T4, respectively. Mulberry leaves were provided five times a day for feeding management. The results obtained showed that the different dosages of propolis used did not affect the weight gain of the larvae, the weight of green cocoons, shells and pupae when compared to the control treatment. However, when comparing the control and T4 from the Spring assay, respectively, to the levels of crude and net silk, significant results were noted. Thus, it can be concluded that propolis glycolic extract in 40-ml dosages may impair the net silk content in a cocoon production, presenting negative results in sericulture...

: Este experimento se llevó a cabo en el Laboratorio de Sericultura, Campus Sede de la Universidad Paranaense (UNIPAR) de Umuarama, en el período de febrero a octubre de 2011, con el fin de verificar el efecto de propóleos en diferentes concentraciones en la alimentación durante el desarrollo biológico del gusano de seda (Bombyx mori L.). El método utilizado en el experimento fue la pulverización de extracto glicólico de propóleos, disuelto en 500 ml de agua destilada en las hojas de morera, en las siguientes dosis, agua control, 25 mL, 30mL, 35 mL, y 40 mL componiendo los tratamientos: control T1 , T2 , T3 e T4 respectivamente. A las hojas de morera se les han dado cinco veces al día, durante el manejo alimentar. Se verificó, por los resultados obtenidos, que las diferentes dosis de propóleos utilizados no afectaron en la ganancia de peso de los gusanos, en el peso de los capullos verdes, en el peso de la cáscara sérica y crisálidas, en comparación con el tratamiento de control, pero cuando se compara el Control y T4 del ensayo de la primavera, respectivamente, para los niveles de seda cruda y líquida, hay resultados significativos. Por lo tanto, se encontró que el extracto glicólico de propóleos, en dosis de 40 mL, puede perjudicar la concentración líquida de seda en una producción de capullos, trayendo resultados poco apreciados dentro de la sericultura...

Animals , Bombyx/growth & development , Bombyx/metabolism , Propylene Glycol/administration & dosage , Propylene Glycol/analysis , Propylene Glycol/adverse effects , Propolis/administration & dosage , Propolis/analogs & derivatives , Propolis/metabolism
Arq. ciênc. vet. zool. UNIPAR ; 17(4): 227-231, out.-dez.2014. tab, graf
Article in English | LILACS | ID: lil-758592


This experiment was carried out with the objective of evaluating different levels of powder propolis in rabbit diets and their effect on semen characteristics. A total of 36 New Zealand White male rabbits were used, randomly distributed into six groups, corresponding to six propolis levels (0, 0.25, 0.50, 0.75, 1.0 and 1.25 g propolis/kg of ration). Semen was collected twice a week, using an artificial vagina. Semen volume, progressive spermatic motility, spermatic vigor, spermatic concentration and spermatic morphology were analyzed. General linear models were used for statistical analysis. The inclusion of powder propolis in the diet increased normal spermatozoa percentage and reduced spermatozoa abnormalities. The powder propolis did not affect the progressive spermatic motility, spermatic vigor or spermatic concentration. The values were considered normal for rabbits. However, a small reduction in semen volume was observed, without any negative effect on the other semen characteristics evaluated. Thus, it is possible to observe better semen quality with the inclusion of 1.25 g powder propolis/kg in the diet for reproducer rabbits...

Este experimento foi realizado com o objetivo de avaliar a influência de diferentes níveis de própolis em pó na ração de coelhos sobre as características do sêmen. Utilizaram-se 36 coelhos machos, adultos, Nova Zelândia Brancos, divididos aleatoriamente em seis grupos, consumindo cinco níveis de própolis (0; 0,25; 0,50; 0,75; 1,0 e 1,25 g de própolis/kg de ração). Coletou-se sêmen duas vezes por semana, utilizando vagina artificial. Verificou-se o volume, a motilidade espermática progressiva, o vigor espermático, a concentração espermática e a morfologia espermática. As análises estatísticas foram realizadas utilizando os modelos lineares generalizados. A adição da própolis na ração elevou a porcentagem de espermatozóides normais e reduziu os anormais. Todavia, foi observada uma pequena redução no volume do sêmen com o aumento do nível de própolis na dieta, sem afetar as demais características do sêmen. A motilidade progressiva, vigor espermático e concentração espermática não foram influenciados pelos diferentes níveis de própolis, valores considerados normais para coelhos. Conclui-se que a melhor qualidade do sêmen de coelhos reprodutores ocorreu com a adição de 1,25 g de própolis/kg de ração...

Este experimento se llevó a cabo para evaluar la influencia de diferentes niveles de polvo de propóleos en la dieta de conejos, bajo las características del semen. Se utilizó 36 conejos machos, adultos, Nueva Zelanda Blancos, divididos al azar en seis grupos, consumiendo cinco niveles de propóleos (0; 0,25; 0,50; 0,75; 1,0 y 1,25g de propóleos/kg en el alimento). Se recogió semen dos veces a la semana, utilizando vagina artificial. Se encontró el volumen, motilidad espermática progresiva, el vigor de espermático, la concentración espermática y la morfología espermática. Los análisis estadísticos se realizaron utilizando modelos lineales generales. La adición de propóleos en la dieta aumentó el porcentaje de espermatozoides normales y redujo los anormales. Sin embargo, se ha observado una pequeña reducción en el volumen del semen con el aumento de propóleos en la dieta, sin afectar las demás características del semen. La motilidad progresiva, vigor espermático y concentración de espermatozoides no se vieron afectados por los diferentes niveles de propóleos, valores considerados normales para conejos. Se concluye que la mejor calidad del semen de conejos reproductores ocurrió con la adición de 1,25g de propóleos / kg en el alimento...

Animals , Male , Propolis/administration & dosage , Propolis/metabolism , Propolis , Animal Feed/analysis , Animal Feed , Semen/chemistry
Article in English | IMSEAR | ID: sea-141240


Objective: This study was conducted to evaluate by clinical and microbiological parameters the effect of subgingival irrigation with propolis extract. Materials and Methods: Twenty patients diagnosed with chronic periodontitis, each presenting three non-adjacent teeth with deep pockets, were selected. Subgingival plaque sampling and clinical recording (at baseline) and scaling and root planing was performed. Two weeks later the selected periodontal sites were submitted to one of the following treatments: Irrigation with a hydroalcoholic solution of propolis extract twice a week for 2 weeks (group A); irrigation with a placebo twice a week for 2 weeks (group B); or no additional treatment (group C). Clinical and microbiological data was collected at baseline and after 4, 6, and 8 weeks. Results: A decrease in the total viable counts of anaerobic bacteria (P=.007), an increase in the proportion of sites with low levels (≤10 5 cfu/mL) of Porphyromonas gingivalis (P=.044), and an increase in the number of sites negative for bleeding on probing was observed in group A sites as compared to group B and C sites. Conclusion: Subgingival irrigation with propolis extract as an adjuvant to periodontal treatment was more effective than scaling and root planing as assessed by clinical and microbiological parameters.

Administration, Topical , Adult , Anti-Infective Agents/administration & dosage , Anti-Infective Agents/therapeutic use , Bacteria/drug effects , Bacteria, Anaerobic/drug effects , Bacterial Load/drug effects , Chronic Periodontitis/microbiology , Chronic Periodontitis/therapy , Combined Modality Therapy , Dental Plaque/microbiology , Dental Scaling , Female , Follow-Up Studies , Gingival Hemorrhage/microbiology , Gingival Hemorrhage/therapy , Humans , Male , Middle Aged , Periodontal Attachment Loss/microbiology , Periodontal Attachment Loss/therapy , Periodontal Index , Periodontal Pocket/microbiology , Periodontal Pocket/therapy , Placebos , Porphyromonas gingivalis/drug effects , Propolis/administration & dosage , Propolis/therapeutic use , Root Planing , Therapeutic Irrigation
Rev. Inst. Med. Trop. Säo Paulo ; 53(6): 329-334, Nov.-Dec. 2011. ilus, graf
Article in English | LILACS | ID: lil-608551


In this study we investigated the efficacy of hyperbaric oxygen (HBO) therapy, alone or combined with the pentavalent antimonial glucantime on Leishmania amazonensis infection. In parallel, the effect of Brazilian red propolis gel (propain) alone or combined with glucantime on L. amazonensis infection was evaluated. The inhibition of the infection in macrophages treated with glucantime in combination with HBO exposition was greater than that of macrophages treated with glucantime alone or HBO alone. The susceptible mouse strain BALB/c infected in the shaved rump with L. amazonensis treated with glucantime and exposed to HBO showed: time points in the course of the disease in which lesions were smaller than those of mice treated with glucantime alone and revascularization of the skin in the lesion site; interferon-gamma (IFN-g) levels were not elevated in lymph node cells from these animals. Propain alone was not efficient against lesions, although less exudative lesions were observed in animals treated with propain alone or combined with glucantime. These results reveal the potential value of HBO and red propolis in combination with glucantime for treating cutaneous leishmaniasis and encourage further studies on the effect of more aggressive HBO, propolis and glucantime therapies on different mouse models of leishmaniasis.

Nesse trabalho foi avaliada a eficácia da terapia da oxigenação hiperbárica (HBO), aplicada em combinação ou não com o tratamento com glucantime, durante a infecção com Leishmania amazonensis. O efeito de gel da própolis vermelha de origem brasileira (propaina) aplicado em combinação ou não com o tratamento com glucantime, também foi avaliado durante infecção com esse parasita. A inibição da infecção de macrófagos tratados com glucantime em combinação com HBO foi maior que a de macrófagos tratados apenas com glucantime ou HBO. A linhagem murina susceptível, BALB/c, infectada no dorso com L. amazonensis, tratada com glucantime e exposta a HBO, mostrou durante o curso da doença, fases em que as lesões eram menores do que a de camundongos apenas tratados com glucantime; observou-se revascularização da pele da lesão e baixa produção de interferon-gama em células de linfonodos desses animais. O tratamento com propaina não foi eficiente na cura das lesões, apesar de lesões menos exsudativas serem observadas em animais tratados com propaina ou propaina combinada ao tratamento com glucantime. Os resultados demonstram que tanto HBO como a própolis vermelha em combinação com glucantime, são promissoras no tratamento da leishmaniose cutânea. Novos estudos devem ser realizados para avaliar tratamentos e outros protocolos em diferentes modelos murinos da leishmaniose.

Animals , Mice , Antiprotozoal Agents/administration & dosage , Hyperbaric Oxygenation/methods , Leishmania mexicana , Leishmaniasis, Cutaneous/therapy , Meglumine/administration & dosage , Organometallic Compounds/administration & dosage , Propolis/administration & dosage , Combined Modality Therapy/methods , Disease Models, Animal , Leishmaniasis, Cutaneous/pathology , Mice, Inbred BALB C , Time Factors
Int. j. morphol ; 29(3): 754-761, Sept. 2011. ilus
Article in Spanish | LILACS | ID: lil-608654


En el presente estudio se evaluó el efecto del propóleos sobre el metabolismo de la glucosa en ratones C57/BL-6 con diabetes mellitus tipo 2 inducida por dieta alta en grasa. Se midieron los cambios en las concentraciones séricas de lípidos, glucosa e insulina, y el efecto sobre la captación de 2-deoxi-[2,6-3H]-D-glucosa, síntesis de [14C]-glicógeno y descarboxilación de [U-14C]-D-glucosa inducida por insulina en músculo aislado. Los resultados muestran que en ratones diabéticos, el tratamiento con propóleos (150 mg/kg/día) reduce los niveles de insulina e índice HOMA (P<0.05). También disminuyó la obesidad abdominal de estos animales (P<0.05). Por otro lado, no modificó las concentraciones plasmáticas de glucosa, colesterol total y triglicéridos. Se observó también que la captación de 2-deoxi-[2,6-3H]-D-glucosa, síntesis de [14C]-glicógeno y descarboxilación de [U-14C]-D-glucosa inducida por insulina en músculo sóleo de ratones tratados con propóleos fue significativamente superior al grupo control (P<0.05). En resumen, nuestros datos confirman que el propóleos es capaz de modular el metabolismo de glucosa en ratones C57/BL-6 con diabetes mellitus tipo 2 inducida por dieta alta en grasa. Los datos obtenidos constituyen un importante antecedente que avala el posible uso del propóleos como fuente de polifenoles con actividad antidiabetogénica.

In the current study, we investigated the effect of propolis on diabetic mice undergoing propolis treatment (150 mg/kg/day) for a 6 week period. We also evaluated serum lipids, glucose, insulin levels and the effect on glucose uptake of 2-deoxy-D-[2,6-3H] glucose, [14C]-glycogen synthesis and [U-14C]-D-glucose decarboxylation induced by insulin in muscle tissue. Our results show that treatment with propolis (150 mg/kg/day) reduced insulin and HOMA index (P<0.05). Propolis also lowered abdominal obesity (P<0.05). No effects over serum glucose, total cholesterol and triglycerides levels were observed. We also observed that uptake of 2-deoxy-D-[2,6-3H] glucose, [14C]-glycogen synthesis and [U-14C]-D-glucose decarboxylation induced by insulin in soleus muscle of mice treated with propolis were significantly greater than control group (P<0.05). In summary, our data establishes that propolis modulates glucose metabolism. This result constitutes important data indicating that propolis can be used as a polyphenols source with antidiabetogenic activity.

Rats , /chemically induced , /metabolism , Propolis/administration & dosage , Propolis/metabolism , Glucose/antagonists & inhibitors , Rats/metabolism
Int. j. morphol ; 29(3): 799-805, Sept. 2011. ilus
Article in Spanish | LILACS | ID: lil-608660


El propóleos se ha utilizado en medicina desde épocas antiguas, una de sus indicaciones en medicina oral y odontológica es como material de recubrimiento directo. Así, el objetivo de este estudio fue cuantificar la neoformación odontoblástica en piezas dentarias de canes en recubrimientos directos. Se realizaron perforaciones en un total de 12 piezas dentarias, la mitad de las cuales fueron recubiertas con propóleos y la otra mitad con hidróxido de calcio. En todos los casos se obturaron las piezas dentarias con cemento de vidrio-ionómero. Al cabo de dos semanas, se realizaron las extracciones de las piezas dentarias, fijándolas en formalina tamponada; se efectuaron cortes histológicos de las piezas, los cuales se tiñeron con hematoxilina-eosina, con el fin de poder realizar la cuantificación de los núcleos odontoblásticos presentes tanto en las zonas alejadas de la perforación, como en las zonas cercanas a las lesiones. Se observó una similar actividad odontoblástica en las zonas cercanas a las lesiones al utilizar cualquiera de los dos materiales (p=0,9325), diferenciándose solamente en la disposición de las células neoformadas, que en el caso de utilizar propóleos fueron en empalizada, semejante a las zonas sanas, en cambio, al utilizar hidróxido de calcio no se observó dicho ordenamiento.

Since ancient times, propolis has been used in medicine, while in oral medicine and dentistry it has been indicated as a direct capping material.The aim of this study was to quantify the odontoblast activity in drilling holes on dog's teeth (canine and incisor) during direct capping.Perforations were carried out in a total sample of 12 dental pieces. In half of the sample teeth were capped with propolis, and in the other half with calcium hydroxide. In all cases teeth were obturated with glass ionomer cement. Extraction of the teeth was realized after two weeks, and teeth were fixed in buffered formalin. Histological cuts of the pieces were made and stained with hematoxylin-eosin in order to carry out quantification of nuclei presence in healthy areas (away from the perforation, as well as in areas adjacent to injuries. Results showed similar odontoblast activity while using either of the materials, (p=0.9325), only differing in the arrangement of newly formed cells which in the cases where propolis was used, were palisading, similar to healthy areas. On the other hand, the same order was not observed when calcium hydroxide was used.

Dogs , Calcium Hydroxide/administration & dosage , Calcium Hydroxide/therapeutic use , Odontoblasts , Odontoblasts/ultrastructure , Dogs/anatomy & histology , Dogs/growth & development , Propolis/administration & dosage , Propolis/therapeutic use , Dental Pulp Capping/veterinary
Pesqui. bras. odontopediatria clín. integr ; 11(01): 123-127, jul. 2011.
Article in Portuguese | LILACS, BBO | ID: lil-614383


Objeti vo: Verifi car, in vitro, o efeito anti microbiano do pólen e dos extratos alcoólico e aquoso da própolis em suas formas pura e diluídas sobre cepas de referência Streptococcus mutans ATCC25175, Streptococcus salivarius ATCC 7073, Streptococcus mitis ATCC 903 e Lactobacillus casei ATCC 9595 pela determinação da Diluição Inibitória Máxima (DIM). Método: Uti lizou-se a clorexidina como controle positivo e água desti lada e álcool de cereais 70% como controles negativos. Efetuou-se a diluição das soluções de 1:1 até 1:64dos extratos alcoólico e aquoso da própolis diluídos em álcool 70% e água desti lada, respecti vamente. O pólen foi diluído em álcool, por ser uma substancia apolar, nas concentrações de 5% (quanti dade presente na composição química da própolis) e 50%. Cada linhagem bacteriana foi reati vada em caldo nutritivo Brain Heart Infusion (BHI) e semeadas as placas com auxílio de swabs, procedendo-se com testes de susceti bilidade, em duplicata, por meio do método da difusão em ágar e técnicado ágar recortado. Em seguida, foram incubadas a 37°C, em microaerofilia, por 48h. Resultados: Constatou-se que todas as diluições da própolis alcoólica inibiram o crescimento bacteriano enquanto a própolis aquosa mostrou os menores resultados tendo efeitoapenas sobre S. miti s na forma pura e nas diluições de 1:1 até 1:4. O pólen a 5% foi efi ciente sobre todas as bactérias, porém o pólen a 50% teve ação apenas sobre S. mitis. Os controlesnegati vos não apresentaram ati vidade. Conclusão: Apesar da própolis e do pólen apresentarem atividade anti microbiana contra as cepas de referência superiorà do placebo, esta, porém, foi inferior à da clorexidina.

Objective: To evaluate, in vitro, the antimicrobial effect of pollen and alcoholic and aqueous propolis extracts in their pure and diluted forms against reference strains Streptococcus mutans ATCC 25175, Streptococcus salivarius ATCC 7073, Streptococcus mitis ATCC 903 and Lactobacillus casei ATCC 9595, by determination of Maximum Inhibitory Dilution (MID). Methods: Chlorhexidine was used as a positive control and distilled water and 70% grain alcohol as negative controls. The alcoholic and aqueous propolis extracts were subjected to dilutions from 1:1 to 1:64 in 70% alcohol and distilled water, respectively. For being an apolar substance, pollen was diluted in alcohol at the concentrations of 5% (amount present in the chemical composition of propolis) and 50%. Each bacterial strain was reactivated in Brain Heart Infusion (BHI) broth and seeded onto plates with swabs, and the susceptibility tests were performed in duplicate by the agar diffusion method using the agar well technique. Next, the plates were incubated at 37øC in microaerophilia during 48 hours. Results: All dilutions of alcoholic propolis extract inhibited the bacterial growth while the aqueous propolis extract showed less efficient results, being effective only against S. mitis in its pure form and in the 1:1 to 1:4 dilutions. Pollen at 5% was efficient against all bacteria, but pollen at 5% had action only against S. mitis. The negative controls did not present antimicrobial activity. Conclusion: The antimicrobial activity of propolis and pollen against the reference strains was higher than that of placebo but lower than that of chlorhexidine.

Biological Factors/analysis , In Vitro Techniques , Culture Media/analysis , Culture Media/toxicity , Propolis/administration & dosage , Propolis/pharmacology , Propolis/therapeutic use , Pollen/microbiology , Pollen/toxicity , Statistical Analysis
Arq. bras. med. vet. zootec ; 62(6): 1359-1366, dez. 2010. ilus, tab
Article in English | LILACS | ID: lil-576033


The therapeutic effects of Argentine propolis ear drop formulation on canine otitis externa were evaluated. Forty-eight dogs with symptoms of otitis externa were randomly assigned to double-blinded, controlled clinical trial to evaluate the efficacy of topical formulation with propolis versus a topical placebo in the treatment of otitis externa. The propolis preparation and placebo were administrated into both external ear canals, twice daily for 14 days. Throughout the study, clinical examination and microbiological analysis of dogs ear exudates were made. The most frequent microorganisms isolated in culture media were: Malassezia pachydermatis (54.2 percent), Staphylococcus aureus (43.8 percent), coagulase-negative Staphylococcus (25.0 percent), Pseudomonas aeruginosa (20.8 percent), Candida albicans (18.8 percent), Proteus mirabilis (16.7 percent), Streptococcus spp. (16.7 percent), Enteroccocus faecalis (12.5 percent), Escherichia coli (12.5 percent), Staphylococcus intermedius (6.3 percent), Klebsiella spp. (4.2 percent), andCandida glabrata (2.1 percent). Whereas the control group did not recover from the infectious ear disease, the propolis preparation exhibited antimicrobial activity against most of the microorganisms isolated from samples of the treated group. In addition, no propolis-adverse effects were observed. This allowed propolis-treated patients to show a significant improvement of the clinical parameters. Thus, this new Argentine propolis ear drop formulation may be used for topical treatment of otitis externa in dogs.

Os efeitos terapêuticos da formulação em gotas óticas de própolis procedentes da Argentina foram avaliados no tratamento da otite externa canina. Quarenta e oito cães com sintomas de otite externa foram distribuídos aleatoriamente em ensaio clínico duplo-cego controlado para avaliar a eficácia da formulação tópica com a própolis contra um placebo tópico no tratamento da otite externa. A preparação de própolis e placebo foi administrada em ambos os canais da orelha externa, duas vezes por dia, durante 14 dias. Ao longo do estudo, os cães foram submetidos a exame físico e à análise microbiológica de exsudatos auriculares. Os mais frequentes microrganismos isolados em meios de cultura foram: Malassezia pachydermatis (54,2 por cento), Staphylococcus aureus (43,8 por cento), Staphylococcus coagulase-negativo (25,0 por cento), Pseudomonas aeruginosa (20,8 por cento), Candida albicans (18,8 por cento), Proteus mirabilis (16,7 por cento), Streptococcus spp.(16,7 por cento), Enterococcus faecalis (12,5 por cento), Escherichia coli (12,5 por cento), Staphylococcus intermedius (6,3 por cento), Klebsiella spp.(4,2 por cento) e Candida glabrata (2,1 por cento). A preparação de própolis apresentou atividade antimicrobiana contra a maioria dos microrganismos isolados de amostras do grupo de tratamento, sendo que os do grupo-controle não se recuperaram da doença infecciosa auricular, e não foram observados efeitos adversos à própolis. Isso permitiu aos pacientes tratados com própolis melhora significativa dos parâmetros clínicos. Essa nova formulação da própolis argentina para o ouvido apresenta potencial utilidade no tratamento tópico da otite externa em cães.

Dogs , Dogs/classification , Otitis Externa/microbiology , Propolis/administration & dosage , Glycerol/administration & dosage , Infections/microbiology , Ear/anatomy & histology
Pakistan Journal of Pharmaceutical Sciences. 2009; 22 (2): 168-174
in English | IMEMR | ID: emr-92343


Free radicals have been implicated in the pathogenesis of diabetes mellitus leading to various complications including atherosclerosis. Propolis was reported to have oxygen radical scavenging activity. The present study was designed to investigate the possible antidiabetic, hypolipidemic and antioxidant effects of ethanolic extract of propolis [EEP]. Type[2] diabetes was induced in rats by injection of streptozotocin [STZ] in a dose of 60 mg/kg bwt, i.p. for 3 consecutive days. After 5 weeks of STZ injection, there were an apparent reduction in the animal body weight amounting to 21% and significant increases in serum glucose [184%], triglycerides [63%], total cholesterol [43%] and low density lipoprotein-cholesterol [LDL-C] [148%] with a concomitant decrease in serum high density lipoprotein-cholesterol [HDL-C] [51%] as compared to the control normal group. In addition, there was significant elevation in pancreatic lipid peroxides measured as malondialdehyde [MDA] and serum nitric oxide [NO] amounting to 185% and 224%, respectively with marked reduction in serum reduced glutathione [GSH] andcatalase [CAT] [66% and 31%, respectively] and pancreatic superoxide dismutase [SOD] [54%] in STZ-treated rats. On the other hand, oral daily treatment of animals with EEP in a dose of 200mg/kg bwt for a period of 5 weeks ameliorated STZ-induced alterations in the animal body weight as well as in serum glucose, lipids, lipoproteins, NO, GSH and CAT and pancreatic MDA and SOD. In conclusion, propolis extract offers promising antidiabetic and hypolipidemic effects that may be mainly attributed to its potent antioxidant potential. Further studies will be needed in future in order to determine which one[or more] of its active constituents has the main antidiabetic and hypolipidemic effects

Animals, Laboratory , Propolis/administration & dosage , Hypoglycemic Agents , Hypolipidemic Agents , Antioxidants , Streptozocin , Diabetes Mellitus, Experimental , Rats, Wistar , Plant Extracts
Pakistan Journal of Pharmaceutical Sciences. 2009; 22 (2): 205-210
in English | IMEMR | ID: emr-92350


Oxidative stress may play a key role in the pathogenesis of diabetic nephropathy. Propolis and its extract have antioxidant properties. The effect of ethanolic extract of propolis against experimental diabetes mellitus-associated changes was examined. Diabetes was induced experimentally in rats by i.p. injection of streptozotocin [STZ] in a dose of 60 mg/kg bwt for 3 successive days. Blood urea nitrogen [BNU], creatinine, glucose, lipid profile, malondialdehyde [MDA] and urinary albumin were measured. Superoxide dimutase [SOD], glutathione [GSH], catalase [CAT] and MDA were measured in the renal tissue. The results showed decreased body weight and increased kidney weight in diabetic animals. Compared to the control normal rats, diabetic rats had higher blood glucose, BNU, creatinine, total cholesterol, triglycerides, low-density lipoprotein-cholesterol [LDL-C], MDA and urinary albumin and lower high-density lipoprotein-cholesterol [HDL-C] levels. Moreover, renal tissue MDA was markedly increased while SOD, GSH and CAT were significantly decreased. Oral administration of propolis extract in doses of 100,200 and 300 mg/kg bwt improved the body and kidney weights, serum glucose, lipid profile, MDA and renal function tests. Renal GSH, SOD and CAT were significantly increased while MDA was markedly reduced. These results may suggest a strong antioxidant effect of propolis which can ameliorate oxidative stress and delay the occurrence of diabetic nephropathy in diabetes mellitus

Animals, Laboratory , Propolis/administration & dosage , Diabetic Nephropathies/physiopathology , Diabetic Nephropathies/prevention & control , Antioxidants , Streptozocin , Free Radicals , Rats, Wistar , Oxidative Stress , Diabetes Mellitus, Experimental
J Vector Borne Dis ; 2008 Dec; 45(4): 301-6
Article in English | IMSEAR | ID: sea-118067


BACKGROUND & OBJECTIVES: Leishmaniasis is a parasitic disease transmitted by sand flies. Many investigations are performed to find an effective and safe treatment for leishmaniasis. In this study, we evaluated the efficacy of herbal extracts of Thymus vulgaris (Thyme) and Achillea millefolium (Yarrow), propolis hydroalcoholic extract and systemic glucantime against cutaneous leishmaniasis in Balb/c mice. METHODS: A total of 45 mice were randomised into five groups each including nine mice. They were treated with pure ethanol 70 degrees, systemic glucantime, Achillea millefolium hydroalcoholic extract, Thymus vulgaris hydroalcoholic extract and propolis hydroalcoholic extract for six weeks. The statistical tests including student t-test were used for analysis. Data were analyzed by SPSS software, ver 13.00. RESULTS: Mean of ulcer size reduction were -17.66, -22.57, 43.29, 36.09 and 43.77% for the alcohol, glucantime, yarrow, thyme and propolis groups, respectively. The results were suggestive that Thymus vulgaris, Achillea millefolium and propolis hydroalcoholic extracts were significantly more effective in reduction of ulcer size as compared with glucantime (p = 0.006, 0.002 and 0.008, respectively). INTERPRETATION & CONCLUSION: Our results are suggestive that Thymus vulgaris, Achillea millefolium and propolis extracts are effective for treatment of cutaneous leishmaniasis in mice. Regarding these results, we suggest that efficacy of these extracts alone or in combination are evaluated against human cutaneous leishmaniasis as a randomized clinical trial.

Achillea , Administration, Cutaneous , Animals , Antiprotozoal Agents/administration & dosage , Female , Injections, Intraperitoneal , Leishmaniasis, Cutaneous/drug therapy , Meglumine/administration & dosage , Mice , Mice, Inbred BALB C , Organometallic Compounds/administration & dosage , Phytotherapy , Plant Extracts/administration & dosage , Propolis/administration & dosage , Thymus Plant , Treatment Outcome
J. appl. oral sci ; 16(4): 275-279, July-Aug. 2008. graf, tab
Article in English | LILACS | ID: lil-486496


The aim of this in vitro study was to determine the maximum inhibitory dilution (MID) of four cetylpyridinium chloride (CPC)-based mouthwashes: CPC+Propolis, CPC+Malva, CPC+Eucaliptol+Juá+Romã+Propolis (Natural Honey®) and CPC (Cepacol®), against 28 Staphylococcus aureus field strains, using the agar dilution method. Decimal dilutions ranging from 1/10 to 1/655,360 were prepared and added to Mueller Hinton Agar. Strains were inoculated using Steers multipoint inoculator. The inocula were seeded onto the surface of the culture medium in Petri dishes containing different dilutions of the mouthwashes. The dishes were incubated at 37ºC for 24 h. For readings, the MID was considered as the maximum dilution of mouthwash still capable of inhibiting microbial growth. The obtained data showed that CPC+Propolis had antimicrobial activity against 27 strains at 1/320 dilution and against all 28 strains at 1/160 dilution, CPC+Malva inhibited the growth of all 28 strains at 1/320 dilution, CPC+Eucaliptol+Juá+Romã+Propolis inhibited the growth of 2 strains at 1/640 dilution and all 28 strains at 1/320 dilution, and Cepacol® showed antimicrobial activity against 3 strains at 1/320 dilution and against all 28 strains at 1/160 dilution. Data were submitted to Kruskal-Wallis test, showing that the MID of Cepacol® was lower than that determined for the other products (p<0.05). In conclusion, CPC-mouthwashes showed antimicrobial activity against S. aureus and the addition of other substances to CPC improved its antimicrobial effect.

Anti-Infective Agents, Local/pharmacology , Cetylpyridinium/pharmacology , Mouthwashes/pharmacology , Staphylococcus aureus/drug effects , Anti-Infective Agents, Local/administration & dosage , Cetylpyridinium/administration & dosage , Cyclohexanols/administration & dosage , Dose-Response Relationship, Drug , Drug Combinations , Malva , Microbial Sensitivity Tests , Monoterpenes/administration & dosage , Mouthwashes/administration & dosage , Plant Oils/administration & dosage , Propolis/administration & dosage
Arq. odontol ; 43(3): 60-66, jul.-set. 2007. tab
Article in Portuguese | LILACS, BBO | ID: lil-533400


Avaliou-se a atuação clínica e microbiológica da solução para bochecho de própolis em crianças cárie ativas. Para tanto, usou-se a solução de fluoreto de sódio a 0,2% como controle positivo. A atividade antimicrobiana do extrato de própolis foi avaliada em meio de cultura sólido para determinar a Concentração Inibitória Mínima (CIM) utilizando cepas de S. mutans. A partir da CIM do extrato, foi confeccionada uma solução para bochecho de própolis a 6,25%. A amostra foi composta de 30 crianças cárie ativas, com idades de 8 a 10 anos, divididas aleatoriamente em: GF-Grupo Flúor (Controle) e GP-Grupo Própolis. Foram coletados índices de biofilme dentário (IHOS e PHP), doença gengival (ISG, IG) e contagem de S. mutans de amostra salivar antes (In-inical) e 24 horas após (Fn-final) a 15ª aplicação das soluções para bochecho. Os resultados mostraram valores In e Fn para GF: IHOS 2,4/2,0; PHP 0,88/0,79; IG 0,34/0,11; ISG 13,22/9,15; e UFC/ml...

Humans , Male , Female , Child , Propolis/administration & dosage , Streptococcus mutans , Mouthwashes
J. venom. anim. toxins ; 1(1): 31-7, 1995. tab, ilus
Article in English | LILACS | ID: lil-194268


Ethanolic extracts of the bee glue, a resinous substance collected by honeybees called propolis, have been widely used in folk medicine since ancient times. Antibacterial, antifungal and thus antiseptic properties may represent the basis for the historical and present use of these extracts in dermatology, against inflammatory conditions and common colds. This work was carried out in order to verify possible biochemical alterations in some seric parameters of propolis-treated rats. It was shown that propolis possesses an antioxidant property and its administration did not affect either amylase and alanine transaminase activities or total protein concentration.

Rats , Alanine Transaminase/drug effects , Amylases/drug effects , Propolis/administration & dosage , Propolis/pharmacology , Proteins , Superoxide Dismutase , Ethanol/administration & dosage