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1.
Electron. j. biotechnol ; 34: 83-90, july. 2018. tab, ilus, graf
Article in English | LILACS | ID: biblio-1047375

ABSTRACT

Background: Although the functional redundancy of catechol 1,2-dioxygenase (C12O) genes has been reported in several microorganisms, limited enzymes were characterised, let alone the advantage of the coexistence of the multiple copies of C12O genes. Results: In this study, four novel C12O genes, designated catA, catAI, catAII and catAIII, in the naphthalene-degrading strain Pseudomonas putida ND6, were cloned and characterised. Phylogenetic analysis of their deduced amino acid sequences revealed that the four C12O isozymes each formed independent subtrees, together with homologues from other organisms. All four enzymes exhibited maximum activity at pH 7.4 and higher activity in alkaline than in acidic conditions. Furthermore, CatA, CatAI and CatAIII were maximally active at a temperature of 45°C, whereas a higher optimum temperature was observed for CatAII at a temperature of 50°C. CatAI exhibited superior temperature stability compared with the other three C12O isozymes, and kinetic analysis indicated similar enzyme activities for CatA, CatAI and CatAII, whereas that of CatAIII was lower. Significantly, among metal ions tested, only Cu2+ substantially inhibited the activity of these C12O isozymes, thus indicating that they have potential to facilitate bioremediation in environments polluted with aromatics in the presence of metals. Moreover, gene expression analysis at the mRNA level and determination of enzyme activity clearly indicated that the redundancy of the catA genes has increased the levels of C12O. Conclusion: The results clearly imply that the redundancy of catA genes increases the available amount of C12O in P. putida ND6, which would be beneficial for survival in challenging environments.


Subject(s)
Pseudomonas putida/enzymology , Pseudomonas putida/genetics , Catechol 1,2-Dioxygenase/genetics , Temperature , Biodegradation, Environmental , Cloning, Molecular , Catechol 1,2-Dioxygenase/analysis , Catechol 1,2-Dioxygenase/metabolism , Genes, Bacterial , Hydrogen-Ion Concentration , Isoenzymes , Metals
2.
Braz. j. microbiol ; 46(3): 649-657, July-Sept. 2015. tab, ilus
Article in English | LILACS | ID: lil-755803

ABSTRACT

To facilitate the biodegradation of diesel oil, an oil biodegradation bacterial consortium was constructed. The alkane hydroxylase (alkB) gene of Pseudomonas putida GPo1 was constructed in a pCom8 expression vector, and the pCom8-GPo1 alkB plasmid was transformed into Escherichia coli DH5α. The AlkB protein was expressed by diesel oil induction and detected through SDS-polyacrylamide gel electrophoresis. The culture of the recombinant (pCom8-GPo1 alkB/E. coli DH5α) with the oil biodegradation bacterial consortium increased the degradation ratio of diesel oil at 24 h from 31% to 50%, and the facilitation rates were increased as the proportion of pCom8-GPo1 alkB/E. coli DH5α to the consortium increased. The results suggested that the expression of the GPo1 gene in E. coli DH5α could enhance the function of diesel oil degradation by the bacterial consortium.

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Subject(s)
Acinetobacter/metabolism , Biodegradation, Environmental , /genetics , Escherichia coli/metabolism , Microbial Consortia/genetics , Organisms, Genetically Modified/metabolism , Pseudomonas putida/enzymology , Acinetobacter/genetics , Escherichia coli/enzymology , Escherichia coli/genetics , Fuel Oils , Gasoline , Genetic Engineering , Oxidation-Reduction , Organisms, Genetically Modified/genetics , Plasmids/genetics , Pseudomonas putida/genetics , Pseudomonas putida/metabolism
3.
Salud pública Méx ; 56(6): 579-591, nov.-dic. 2014. ilus, tab
Article in English | LILACS | ID: lil-733352

ABSTRACT

Objective. To conduct a health impact assessment (HIA) to quantify health benefits for several PM and O3 air pollution reduction scenarios in the Mexico City Metropolitan Area (MCMA). Results from this HIA will contribute to the scientific support of the MCMA air quality management plan (PROAIRE) for the period 2011-2020. Materials and methods. The HIA methodology consisted of four steps: 1) selection of the air pollution reduction scenarios, 2) identification of the at-risk population and health outcomes for the 2005 baseline scenario, 3) selection of concentration-response functions and 4) estimation of health impacts. Results. Reductions of PM10 levels to 20 μg/m³ and O3 levels to 0.050ppm (98 µg/m³) would prevent 2300 and 400 annual deaths respectively. The greatest health impact was seen in the over-65 age group and in mortality due to cardiopulmonary and cardiovascular disease. Conclusion. Improved air quality in the MCMA could provide significant health benefits through focusing interventions by exposure zones.


Objetivo. Realizar una evaluación de impacto en salud (EIS) que documente los beneficios en salud ante diversos escenarios de reducción de PM10 y O3 en el aire de la Zona Metropolitana del Valle de México (ZMVM). Los resultados contribuyen al sustento científico del plan de gestión de calidad del aire (PROAIRE 2011-2020). Material y métodos. La metodología de EIS comprende cuatro pasos: 1) selección de los escenarios de reducción, 2) identificación de la población en riesgo y de los eventos en salud para el año basal 2005, 3) selección de las funciones de concentración-respuesta y 4) estimación del impacto en la salud. Resultados. Reducciones de PM10 a 20μg/m³ y de O3 a 0.050ppm (98 µg/m³) evitarían, respectivamente, cerca de 2 300 y 400 muertes por año. El mayor impacto se observa en el grupo de más de 65 años y en la mortalidad por causas cardiopulmonares y cardiovasculares. Conclusiones. Mejorar la calidad del aire en la ZMVM podría reflejar importantes beneficios para la salud focalizados por zonas o áreas de exposición.


Subject(s)
Pseudomonas putida/metabolism , Styrenes/metabolism , Aldehyde Oxidoreductases/metabolism , Biodegradation, Environmental , Epoxy Compounds/metabolism , Escherichia coli Proteins , Glutamic Acid/metabolism , Isomerases/metabolism , Oxidation-Reduction , Oxygen Consumption , Phenylacetates/metabolism , Pseudomonas putida/enzymology , Pseudomonas putida/growth & development , Styrene , Succinates/metabolism , Succinic Acid
4.
Salud colect ; 10(3): 325-337, sep.-dic. 2014. tab
Article in Spanish | LILACS | ID: lil-733293

ABSTRACT

En el marco de la creciente feminización de la profesión médica en México, el artículo indaga sobre las características de este proceso para el caso de la ginecobstetricia. Considerando la feminización como un proceso de cambio, que se analiza cuantitativa y cualitativamente, el artículo se detiene en especial en las experiencias de las mujeres ginecobstetras, experiencias que se dan en el seno de una especialidad que, desde sus orígenes, funcionó como un dispositivo de control del cuerpo de las mujeres. Basado en una investigación etnográfica, el artículo combina fuentes estadísticas, de archivo y de observación de campo. El material que surge de las entrevistas muestra las experiencias y tensiones que viven las ginecobstetras en este contexto.


In the framework of an increasing feminization of the medical profession in Mexico, this article explores the characteristics of this process in the obstetrics and gynecology specialty. Understanding feminization as a process of change to be analyzed both quantitatively and qualitatively, the article focuses special attention on the experiences of female obstetrician-gynecologists within a medical specialty that has since its origins functioned as a mechanism of control over women's bodies. Based on ethnographic research, the article combines statistical and archival sources and field observation. The interviews reveal the experiences and tensions women obstetrician-gynecologists encounter in this context.


Subject(s)
Alcohol Oxidoreductases/chemistry , Alcohol Oxidoreductases/metabolism , Arginine/chemistry , Pseudomonas putida/enzymology , Alcohol Oxidoreductases/genetics , Alcohol Oxidoreductases/isolation & purification , Binding, Competitive/genetics , Catalysis , Enzyme Activation/genetics , Flavin Mononucleotide/metabolism , Kinetics , Ligands , Mandelic Acids/metabolism , Mutagenesis, Site-Directed , Phenylacetates/metabolism , Protein Binding/genetics , Pseudomonas putida/genetics , Substrate Specificity/genetics , Sulfites/metabolism
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