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1.
Article in English | WPRIM | ID: wpr-764304

ABSTRACT

BACKGROUND: NAD(P)H:quinone oxidoreductase-1 (NQO1) is a widely-distributed flavin adenine dinucleotide-dependent flavoprotein that promotes obligatory 2-electron reductions of quinones, quinoneimines, nitroaromatics, and azo dyes. This reduces quinone levels and thereby minimizes generation of excess reactive oxygen species (ROS) formed by redox cycling, and concurrent depletion of intracellular thiol pools. Ajoene is derived from crushed garlic. It is formed by a reaction involving two allicin molecules, and is composed of allyl sulfide and vinyl disulfide. Ajoene is present in two isomers, E- and Z-form. METHODS: Expression of antioxidant enzymes and nuclear factor E2-related factor-2 (Nrf2) was measured by Western blot analysis. NQO1 promoter activity was assessed by the luciferase reporter gene assay. ROS accumulation was monitored by using the fluorescence-generating probe 2′,7′-dichlorofluorescein diacetate. The intracellular glutathione levels were measured by using a commercially available kit. RESULTS: Z-ajoene significantly up-regulated the expression of representative antioxidant enzyme NQO1 in non-tumorigenic breast epithelial MCF-10A cells at non-toxic concentrations. Z-ajoene enhanced up-regulation and nuclear translocation of Nrf2, which plays a pivotal role in the induction of many genes encoding antioxidant enzymes and other cytoprotective proteins. Z-ajoene treatment also increased the activity of nqo1-promoter harboring antioxidant response element consensus sequences in MCF-10A cells. Silencing of Nrf2 by small interfering RNA abrogated ajoene-induced expression of NQO1. Z-ajoene activated extracellular signal-regulated kinase (ERK). Inhibition of ERK activation by U0126 abrogated ability of Z-ajoene to activate Nrf2 and to induce NQO1 expression. Intracellular ROS accumulation was observed after treatment with Z-ajoene, whereas the E-isoform was not effective. The inhibition of ROS by treatment with N-acetylcysteine, a radical scavenger, abrogated Z-ajoene-induced expression of NQO1 as well as activation of ERK and Nrf2, suggesting that Z-ajoene augments the Nrf2-dependent antioxidant defense via ROS generation and ERK activation. CONCLUSIONS: Z-ajoene induces NQO1 expression in MCF-10A cells through ROS-mediated activation of Nrf2.


Subject(s)
Acetylcysteine , Adenine , Antioxidant Response Elements , Azo Compounds , Blotting, Western , Breast , Consensus Sequence , Epithelial Cells , Flavoproteins , Garlic , Genes, Reporter , Glutathione , Humans , Luciferases , NF-E2-Related Factor 2 , Oxidation-Reduction , Phosphotransferases , Quinones , Reactive Oxygen Species , RNA, Small Interfering , Up-Regulation
2.
Article in English | WPRIM | ID: wpr-287148

ABSTRACT

<p><b>OBJECTIVE</b>This study observed attenuating effect of hydroxysafflor yellow A (HSYA), an effective ingredient of aqueous extract of Carthamus tinctorius L, on lipopolysaccharide (LPS)-induced endothelium inflammatory injury.</p><p><b>METHODS</b>Eahy926 human endothelium cell (EC) line was used; thiazolyl blue tetrazolium bromide (MTT) test was assayed to observe the viability of EC; Luciferase reporter gene assay was applied to measure nuclear factor-κB (NF-κB) p65 subunit nuclear binding activity in EC; Western blot technology was used to monitor mitogen activated protein kinase (MAPKs) and NF-κB activation. Reverse transcription polymerase chain reaction (RT-PCR) method was applied to observe intercellular cell adhesion molecule-1 (ICAM-1) and E-selectin mRNA level; EC surface ICAM-1 expression was measured with flow cytometry and leukocyte adhesion to EC was assayed with Rose Bengal spectrophotometry technology.</p><p><b>RESULTS</b>HSYA protected EC viability against LPS-induced injury (P <0.05). LPS-induced NF-κB p65 subunit DNA binding (P <0.01) and nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor α (IκBα) phosphorylation was inhibited by HSYA. HSYA attenuated LPS triggered ICAM-1 and E-selectin mRNA levels elevation and phosphorylation of p38 MAPK or c-Jun N-terminal kinase MAPK. HSYA also inhibited LPS-induced cell surface ICAM-1 protein expression P <0.01) and leukocyte adhesion to EC (P <0.05).</p><p><b>CONCLUSION</b>HSYA is effective to protect LPS-induced high expression of endothelium adhesive molecule and inflammatory signal transduction.</p>


Subject(s)
Cell Adhesion , Cell Nucleus , Metabolism , Cell Survival , Chalcone , Chemistry , Pharmacology , Therapeutic Uses , E-Selectin , Genetics , Metabolism , Endothelium, Vascular , Pathology , Gene Expression Regulation , Human Umbilical Vein Endothelial Cells , Metabolism , Pathology , Humans , I-kappa B Proteins , Metabolism , Inflammation , Drug Therapy , Pathology , Intercellular Adhesion Molecule-1 , Genetics , Metabolism , Leukocytes , Cell Biology , Lipopolysaccharides , MAP Kinase Signaling System , NF-KappaB Inhibitor alpha , Phosphorylation , Protective Agents , Pharmacology , Protein Binding , Quinones , Chemistry , Pharmacology , Therapeutic Uses , RNA, Messenger , Genetics , Metabolism
3.
Article in English | WPRIM | ID: wpr-125743

ABSTRACT

BACKGROUND: Idiopathic pulmonary fibrosis is a common interstitial lung disease; it is a chronic, progressive, and fatal lung disease of unknown etiology. Over the last two decades, knowledge about the underlying mechanisms of pulmonary fibrosis has improved markedly and facilitated the identification of potential targets for novel therapies. However, despite the large number of antifibrotic drugs being described in experimental pre-clinical studies, the translation of these findings into clinical practices has not been accomplished yet. NADH:quinone oxidoreductase 1 (NQO1) is a homodimeric enzyme that catalyzes the oxidation of NADH to NAD+ by various quinones and thereby elevates the intracellular NAD⁺ levels. In this study, we examined the effect of increase in cellular NAD⁺ levels on bleomycin-induced lung fibrosis in mice. METHODS: C57BL/6 mice were treated with intratracheal instillation of bleomycin. The mice were orally administered with β-lapachone from 3 days before exposure to bleomycin to 1-3 weeks after exposure to bleomycin. Bronchoalveolar lavage fluid (BALF) was collected for analyzing the infiltration of immune cells. In vitro, A549 cells were treated with transforming growth factor β1 (TGF-β1) and β-lapachone to analyze the extracellular matrix (ECM) and epithelial-mesenchymal transition (EMT). RESULTS: β-Lapachone strongly attenuated bleomycin-induced lung inflammation and fibrosis, characterized by histological staining, infiltrated immune cells in BALF, inflammatory cytokines, fibrotic score, and TGF-β1, α-smooth muscle actin accumulation. In addition, β-lapachone showed a protective role in TGF-β1–induced ECM expression and EMT in A549 cells. CONCLUSION: Our results suggest that β-lapachone can protect against bleomycin-induced lung inflammation and fibrosis in mice and TGF-β1–induced EMT in vitro, by elevating the NAD+/NADH ratio through NQO1 activation.


Subject(s)
Actins , Animals , Bleomycin , Bronchoalveolar Lavage Fluid , Cytokines , Epithelial-Mesenchymal Transition , Extracellular Matrix , Fibrosis , Idiopathic Pulmonary Fibrosis , In Vitro Techniques , Inflammation , Lung Diseases , Lung Diseases, Interstitial , Lung , Mice , NAD , Pneumonia , Pulmonary Fibrosis , Quinones , Transforming Growth Factor beta1 , Transforming Growth Factors
4.
Chinese Journal of Cardiology ; (12): 728-731, 2015.
Article in Chinese | WPRIM | ID: wpr-351613

ABSTRACT

<p><b>OBJECTIVE</b>To elucidate the effect of hydroxysafflor yellow A ( HYSA) on the proliferation of vascular smooth muscle cells (VSMCs) and the related mechanism.</p><p><b>METHODS</b>VSMCs derived from SD rats were treated with DMEC culture medium (Control), 10 ng/ml PDGF (PDGF group), pretreatment with HYSA at different doses (1, 5, 10, 20, 40, 60 µmol/L) for 24 h then cotreatment with PDGF. After 24 h, MTT assay, Western blot and immunohistochemical staining were performed to evaluate the inhibitory effects of HYSA on VSMCs proliferation.</p><p><b>RESULTS</b>HYSA inhibited PDGF induced VSMCs proliferation in a dose-dependent manner, dowregulated proliferating cell nuclear antigen (PCNA) expression and blocked PDGF activated PDGFR-MEK-ERK1/2 signaling pathway.</p><p><b>CONCLUSIONS</b>HYSA inhibits VSMCs proliferation possibly via downregulating the expression of PCNA and blocking MEK-ERK1/2 signal transduction in VSMCs.</p>


Subject(s)
Animals , Cell Proliferation , Cells, Cultured , Chalcone , MAP Kinase Signaling System , Mitogen-Activated Protein Kinase 1 , Mitogen-Activated Protein Kinase 3 , Mitogen-Activated Protein Kinases , Muscle, Smooth, Vascular , Myocytes, Smooth Muscle , Proliferating Cell Nuclear Antigen , Quinones , Rats , Rats, Sprague-Dawley
5.
Article in English | WPRIM | ID: wpr-812509

ABSTRACT

The ocean continues to provide a plethora of unique scaffolds capable of remarkable biological applications. A large number of pyrroloiminoquinone alkaloids, including discorhabdins, epinardins, batzellines, makaluvamines, and veiutamine, have been isolated from various marine organisms. A class of pyrroloiminoquinone-related alkaloids, known as bispyrroloquinones, is the focus of this review article. This family of marine alkaloids, which contain an aryl substituted bispyrroloquinone ring system, includes three subclasses of alkaloids namely, wakayin, tsitsikammamines A-B, and zyzzyanones A-D. Both wakayin and the tsitsikammamines contain a tetracyclic fused bispyrroloiminoquinone ring system, while zyzzyanones contain a fused tricyclic bispyrroloquinone ring system. The unique chemical structures of these marine natural products and their diverse biological properties, including antifungal and antimicrobial activity, as well as the potent, albeit generally nonspecific and universal cytotoxicities, have attracted great interest of synthetic chemists over the past three decades. Tsitsikammamines, wakayin, and several of their analogs show inhibition of topoisomerases. One additional possible mechanism of anticancer activity of tsitsikammamines analogs that has been discovered recently is through the inhibition of indoleamine 2, 3-dioxygenase, an enzyme involved in tumoral immune resistance. This review discusses the isolation, synthesis, and evaluation of bioactivities of bispyrroloquinone alkaloids and their analogs.


Subject(s)
Alkaloids , Chemistry , Pharmacology , Animals , Anti-Infective Agents , Chemistry , Pharmacology , Antineoplastic Agents , Chemistry , Pharmacology , Biological Products , Chemistry , Pharmacology , Humans , Indole Alkaloids , Chemistry , Pharmacology , Indoles , Chemistry , Pharmacology , Pyrroles , Chemistry , Pharmacology , Quinolines , Chemistry , Pharmacology , Quinones , Chemistry , Pharmacology
6.
Mycobiology ; : 157-162, 2015.
Article in English | WPRIM | ID: wpr-729847

ABSTRACT

Lichen-forming fungal proteins have been seldom searched due to many difficulties in their extraction. Phenols, quinones, proteases, and other components released during cell disruption have been known to be the greatest challenges related to protein extraction from lichens. To overcome these problems and maintain good electrophoretic resolution and high protein concentration, an extraction buffer containing polyvinylpolypyrrolidone, ascorbic acid, Triton X-100, polyethylene glycol, proteinase, and oxidase inhibitors in sodium phosphate buffer was developed. This extraction buffer showed high efficiency for all lichen species tested in the study.


Subject(s)
Ascorbic Acid , Electrophoresis , Fungal Proteins , Lichens , Octoxynol , Oxidoreductases , Peptide Hydrolases , Phenol , Phenols , Polyethylene Glycols , Quinones , Sodium
8.
Bol. latinoam. Caribe plantas med. aromát ; 13(6): 566-574, nov.2014. ilus, tab
Article in English | LILACS | ID: lil-795827

ABSTRACT

The synthesis of new isomeric ellipticine quinones 3a-c and their in vitro antiproliferative activities on cancer cell lines is reported. The designed N-heterocyclic quinones 3a-c were synthesized through a three step sequence which involves: a) one-pot preparation of 4-methoxycarbonyl-3,4-dimethylisoquinoline-5,8-quinone 1 from 2,5-dihydroxyacetophenone, methyl aminocrotonate and silver (II) oxide; b) regioselective amination of 1 with arylamines to give aminoquinones 2a-c and c) palladium-catalyzed intramolecular oxidative coupling of 7-aminoisoquinoline-5,8-quinones 2a-c. The in vitro antiproliferative activity of the new angular quinones was evaluated againts one normal cell line (lung fibroblasts) and gastric, lung and bladder cancer cell lines in 72-h drug exposure assays. The new compounds displayed similar or higher antiproliferative activity with respect to their quinone precursors 2a-c. The isomeric ellipticine quinone 2b appears as the more active member on bladder cancer cell line (IC50: 2.4 uM), comparable to etoposide used as anticancer reference drug...


Se describe la síntesis de las nuevas quinonas 3a-c, isoméricas de elipticina, y sus actividades antiproliferativas in vitro en líneas de células de cáncer. Las quinonas N-heterocíclicas 3a-c se sintetizaron a través de una secuencia que involucra: a) preparación de 4- metoxicarbonil-3,4-dimetlisoquinolin-5,8-quinone 1 a partir de 2,5-dihidroxiacetofenona, aminocrotonato de metilo y óxido de plata (I); b) aminación regioselectiva de 1 con arilaminas para producir las aminoquinonas 2a-c y c) acoplamiento oxidante intramolecular de 7- aminoisoquinolin-5,8-quinonas 2a-c catalizado con paladio. La actividad antiproliferative in vitro de los nuevos compuestos fue evaluada en una línea celular normal (fibroblastos de pulmón) y líneas de células de cáncer gástrico, pulmón y vejiga en ensayos de exposición de 72 horas a la droga. Las quinonas 3a-c exhiben interesantes propiedades antiproliferativas destacando la elipticinquinona isomérica 2b en células de cáncer de vejiga (IC50: 2.4 uM) comparado con etopósido usada como droga anticancer de referencia. Los nuevos compuestos mostraron actividades antiproliferativa similar o mayor respecto de las correspondientes quinonas precursoras 2a-c. La elipticin quinona isomérica 2b corresponde al miembro más activo en células de câncer de vejiga (IC50: 2.4 uM), comparable a la del etopósido, usada como droga anticáncer de referencia...


Subject(s)
Humans , Ellipticines/pharmacology , Ellipticines/chemical synthesis , Cell Proliferation , Quinones/pharmacology , Quinones/chemical synthesis , Cell Line, Tumor , Oxidative Coupling
9.
Braz. j. microbiol ; 45(3): 985-993, July-Sept. 2014. mapas, tab
Article in English | LILACS | ID: lil-727030

ABSTRACT

In the present study, samples of rhizosphere and root nodules were collected from different areas of Pakistan to isolate plant growth promoting rhizobacteria. Identification of bacterial isolates was made by 16S rRNA gene sequence analysis and taxonomical confirmation on EzTaxon Server. The identified bacterial strains were belonged to 5 genera i.e. Ensifer, Bacillus, Pseudomona, Leclercia and Rhizobium. Phylogenetic analysis inferred from 16S rRNA gene sequences showed the evolutionary relationship of bacterial strains with the respective genera. Based on phylogenetic analysis, some candidate novel species were also identified. The bacterial strains were also characterized for morphological, physiological, biochemical tests and glucose dehydrogenase (gdh) gene that involved in the phosphate solublization using cofactor pyrroloquinolone quinone (PQQ). Seven rhizoshperic and 3 root nodulating stains are positive for gdh gene. Furthermore, this study confirms a novel association between microbes and their hosts like field grown crops, leguminous and non-leguminous plants. It was concluded that a diverse group of bacterial population exist in the rhizosphere and root nodules that might be useful in evaluating the mechanisms behind plant microbial interactions and strains QAU-63 and QAU-68 have sequence similarity of 97 and 95% which might be declared as novel after further taxonomic characterization.


Subject(s)
Bacteria/classification , Bacteria/isolation & purification , Plant Roots/microbiology , Soil Microbiology , Bacterial Typing Techniques , Bacteria/genetics , Cluster Analysis , Cytosol/chemistry , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Genes , Glucose 1-Dehydrogenase/genetics , Molecular Sequence Data , Pakistan , Phylogeny , Plants , Quinones/analysis , Rhizosphere , /genetics , Sequence Analysis, DNA
10.
Braz. j. microbiol ; 45(2): 603-611, Apr.-June 2014. ilus, tab
Article in English | LILACS | ID: lil-723124

ABSTRACT

Glucose dehydrogenase (GDH; EC 1.1. 5.2) is the member of quinoproteins group that use the redox cofactor pyrroloquinoline quinoine, calcium ions and glucose as substrate for its activity. In present study, Leclercia sp. QAU-66, isolated from rhizosphere of Vigna mungo, was characterized for phosphate solubilization and the role of GDH in plant growth promotion of Phaseolus vulgaris. The strain QAU-66 had ability to solubilize phosphorus and significantly (p < 0.05) promoted the shoot and root lengths of Phaseolus vulgaris. The structural determination of GDH protein was carried out using bioinformatics tools like Pfam, InterProScan, I-TASSER and COFACTOR. These tools predicted the structural based functional homology of pyrroloquinoline quinone domains in GDH. GDH of Leclercia sp. QAU-66 is one of the main factor that involved in plant growth promotion and provides a solid background for further research in plant growth promoting activities.


Subject(s)
Enterobacteriaceae/enzymology , Enterobacteriaceae/physiology , Glucose 1-Dehydrogenase/genetics , Nerve Growth Factors , Phaseolus/growth & development , Phaseolus/microbiology , Cluster Analysis , Computational Biology , Cytosol/chemistry , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , Enterobacteriaceae/genetics , Enterobacteriaceae/isolation & purification , Glucose 1-Dehydrogenase/chemistry , Models, Molecular , Molecular Sequence Data , Phylogeny , Protein Conformation , Protein Structure, Tertiary , Phosphorus/metabolism , Plant Roots/growth & development , Plant Shoots/growth & development , Quinones/analysis , Sequence Analysis, DNA , Sequence Homology
11.
Article in Chinese | WPRIM | ID: wpr-244577

ABSTRACT

Bio-active components from Carthamus tinctorius were separated on the basis of antioxidant capacities in vitro. The antioxidant capacity was investigated on the basis of the ability to scavenge DPPH radical, ABTS radical and reduce Fe3+ of different polar fractions. Furthermore, the chemical compounds were isolated from bio-active fraction, and were evaluated for the antioxidative effects. Five major components were isolated and identified from water extract as 6-hydroxykaempferol 3,6,7-tri-O-β-D-glucoside(1), 6-hydroxykaempferol 3-O-β-rutinoside-6-O-β-D-glucoside (2), 6-hydroxykaempferol 3-O-β-D-glucoside (3), hydroxysafflor yellow A (4) and anhydrosafflor yellow B (5). By evaluating and comparing the antioxidative effects of different fractions and obtained compounds, the results showed that water extract displayed significantly high antioxidative activities and 6-hydroxykaempferol glycosides and quinochalcone C-glycosides were found as main contribution for antioxidant property.


Subject(s)
Antioxidants , Metabolism , Pharmacology , Benzothiazoles , Metabolism , Biphenyl Compounds , Metabolism , Carthamus tinctorius , Chemistry , Chalcone , Metabolism , Pharmacology , Ferric Compounds , Metabolism , Free Radicals , Metabolism , Kaempferols , Metabolism , Pharmacology , Oxidation-Reduction , Picrates , Metabolism , Plant Extracts , Metabolism , Pharmacology , Plants, Medicinal , Chemistry , Quinones , Metabolism , Pharmacology , Sulfonic Acids , Metabolism , Water , Chemistry
12.
Acta Pharmaceutica Sinica ; (12): 1136-1142, 2014.
Article in Chinese | WPRIM | ID: wpr-299156

ABSTRACT

The effect of amygdalin joint hydroxysafflor yellow A (HSYA) on the endplate chondrocytes derived from intervertebral discs of rats induced by IL-1beta and the possible mechanism were studied and explored. Chondrocytes were obtained from endplate of one-month SD rat intervertebral discs and cultured primary endplate chondrocytes. After identification, they were divided into normal group, induced group, amygdalin group, HSYA group and combined group. CCK-8 kit was adopted to detect the proliferation of the endplate chondrocytes. FCM was measured to detect the apoptosis. Real-time PCR method was adopted to observe the mRNA expression of Aggrecan, Col 2 alpha1, Col 10 alpha1, MMP-13 and the inflammatory cytokines IL-1beta. The protein expression of Col II, Col X was tested through immunofluorescence. Compared with the normal group, the proliferation of the endplate chondrocytes decreased while the apoptosis increased (P < 0.05). With down regulation of the mRNA expressions of Aggrecan, Col 2 alpha1 and up regulation of the mRNA expressions of Col 10 alpha1, MMP-13, IL-1beta (P < 0.05), the protein expression of Col II decreased while the protein expression of Col X increased. Compared with the induced group, amygdalin group, HSYA group, the combined group could inhibit the apoptosis and promote the proliferation (P < 0.05). They could increase the mRNA expressions of Aggrecan and Col 2 alpha1 while decrease the mRNA expressions of Col 10 alpha1, MMP-13 and IL-1beta (P < 0.05). They could also enhance the protein expression of Col II while reduce the protein expression of Col X. The effect of the combined group was significantly better than that of amygdalin and HSYA. Amygdalin joint HSYA could inhibit the degeneration of the endplate chondrocytes derived from intervertebral discs of rats induced by IL-1beta and better than the single use of amygdalin or HSYA.


Subject(s)
Amygdalin , Pharmacology , Animals , Apoptosis , Cells, Cultured , Chalcone , Pharmacology , Chondrocytes , Collagen , Metabolism , Drug Synergism , Interleukin-1beta , Intervertebral Disc , Cell Biology , Quinones , Pharmacology , Rats
13.
Article in Chinese | WPRIM | ID: wpr-294049

ABSTRACT

<p><b>OBJECTIVE</b>To develop an HPLC method to determine the contents of danshensu, hydroxysafflor yellow A, rosmarinic acid, lithospermic acid, salvianolic acid B in the water extract of mixed Salviae Miltiorrhizae Radix et Rhizoma and Carthami Flos simultaneously.</p><p><b>METHOD</b>The separation were carried out at 30 degrees C on a ZORBAX Eclipse Plus C18 column (4.6 mm x 100 mm, 1.8 microm) with formic acid-500 mmol x L(-1) ammonium formate-water solution (0.5:10:90) as mobile phase A and acetonitrile-formic acid solution (100: 0.5) as mobile phase B in gradient mode at a flow rate of 0.5 mL x min(-1). Detection wavelengths were 280 nm for danshensu, rosmarinic acid, lithospermic acid, salvianolic acid B, and 380 nm for hydroxysafflor yellow A.</p><p><b>RESULT</b>The 5 components were separated well with a good linearity (R2 > 0.999 3) in the range of the test concentration. The average recoveries of danshensu, hydroxysafflor yellow A, rosmarinic acid, lithospermic acid, and salvianolic acid B were 99.1%, 102%, 102%, 98.5% and 101%, respectively.</p><p><b>CONCLUSION</b>This method is simple, accurate, and repeatable.</p>


Subject(s)
Benzofurans , Chalcone , Chromatography, High Pressure Liquid , Methods , Cinnamates , Depsides , Lactates , Quinones , Rhizome , Chemistry , Salvia miltiorrhiza , Chemistry
14.
Article in Chinese | WPRIM | ID: wpr-318680

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the pharmacokinetic effect of Sappan Lignum on hydroxysafflor yellow A (HSYA) in Carthami Flos.</p><p><b>METHOD</b>Concentration of HSYA in rat plasma was detected by RP-HPLC after rats were orally administered with extracts of Carthami Flos or Carthami Flos combined with Sappan Lignum. Pharmacokinetic parameters were calculated by DAS 2.0 pharmacokinetic software.</p><p><b>RESULT</b>In vivo pharmacokinetic models of HSYA were two-compartment open models in both of the Carthami Flos group and the Carthami Flos combined with Sappan Lignum group. After compatibility, HSYA showed a significant lower in apparent volumes of distribution of t(1/2Ka), t(1/2alpha) and V1/F, with slight advance in T(max).</p><p><b>CONCLUSION</b>Sappan Lignum can accelerate absorption, distribution and metabolic process of HSYA in vivo and reduce its accumulation in vivo.</p>


Subject(s)
Administration, Oral , Animals , Caesalpinia , Chemistry , Carthamus tinctorius , Chemistry , Chalcone , Pharmacokinetics , Chromatography, High Pressure Liquid , Drug Synergism , Drugs, Chinese Herbal , Pharmacokinetics , Female , Flowers , Chemistry , Male , Quinones , Pharmacokinetics , Rats , Rats, Sprague-Dawley , Specific Pathogen-Free Organisms , Wood , Chemistry
15.
Article in Chinese | WPRIM | ID: wpr-346484

ABSTRACT

<p><b>OBJECTIVE</b>To screen out the main components with no significant difference with Salvia miltiorrhiza diterpene quinones pharmacological action, in order to determine the compatible form of representative components that can describe the overall property of S. miltiorrhiza diterpene quinones.</p><p><b>METHOD</b>According to the results of the in vitro pharmacological experiment, the myocardial ischemia model of rats was induced through intraperitoneal injection of isoproterenol. The pharmacologic effects of S. miltiorrhiza diterpene quinones, combination with principal component A and combination with principal component B were compared in electrocardiogram (changes in J point), enzymology indicators (SOD, MDA, CK, LDH) and pathology (myocardial histological changes), so as to screen out the compatible form of representative components that can describe the overall property of S. miltiorrhiza diterpene quinones.</p><p><b>RESULT</b>The S. miltiorrhiza diterpenoid quinone high-dose group and the B high-dose group were similar in all pharmacological effects, with equal efficacy but no significant difference.</p><p><b>CONCLUSION</b>The S. miltiorrhiza diterpenoid quinone high-dose group and the B high-dose group showed a certain therapeutic effect on ISO-induced myocardial ischemia. Therefore, the four components in the B high-dose group can be used as representative components of S. miltiorrhiza diterpene quinones.</p>


Subject(s)
Animals , Diterpenes , Pharmacology , Drug Evaluation, Preclinical , Isoproterenol , Pharmacology , Male , Myocardial Ischemia , Drug Therapy , Quinones , Pharmacology , Rats , Rats, Sprague-Dawley , Salvia miltiorrhiza , Chemistry
16.
Article in Chinese | WPRIM | ID: wpr-288642

ABSTRACT

<p><b>OBJECTIVE</b>To develop a high-performance liquid chromatography (HPLC) method for simultaneous determination of hypocrellin A, hypocrellin B, and hypocrellin C.</p><p><b>METHOD</b>The separation was carried out on a Kromasil C18 (4.6 mm x 250 mm, 5 micrm) colum eluted with in mobile phases of water containing 0.5% glacial acetic acid and acetonitrile. The column temperature was 35 degrees C, and the flow rate was 1.0 mL x min(-1). The detection wavelength was set at 265 nm.</p><p><b>RESULT</b>The three compounds were well separated. Calibration curves of hypocrellin A, hypocrellin B, and hypocrellin C showed good linear relationship RSD > 2.0%. The average recoveries of the hypocrellin A, hypocrellin B, and hypocrellin C were 101.8%, 102.3%, 100.0%, respectively.</p><p><b>CONCLUSION</b>The developed method is simple, accurate, and repeatable, and can be readily used as valid tool for the quality control of Hypocrella bambusae.</p>


Subject(s)
Chromatography, High Pressure Liquid , Methods , Drugs, Chinese Herbal , Hypocreales , Chemistry , Perylene , Quinones
17.
Article in English | WPRIM | ID: wpr-812708

ABSTRACT

AIM@#To study the metabolites of a halotolerant fungus Alternaria sp. M6.@*METHODS@#The metabolites were isolated and purified by various chromatographic techniques. Their structures were determined on the basis of physical properties and spectroscopic data.@*RESULTS@#Nine compounds were isolated and identified as 8β-chloro-3, 6aα, 7β, 9β, 10-pentahydroxy-9, 8, 7, 6a-tetrahydroperylen-4(6aH)-one (1), alterperylenol (2), dihydroalterperylenol (3), adenine (4), adenosine (5), deoxyadenosine (6), guanosine (7), tryptophan (8), and hexadecanoic acid (9).@*CONCLUSION@#Compound 1 is a new perylenequinone.


Subject(s)
Alternaria , Chemistry , Metabolism , Biological Products , Chemistry , Molecular Structure , Perylene , Chemistry , Quinones , Chemistry , Salt Tolerance
18.
Article in Chinese | WPRIM | ID: wpr-346884

ABSTRACT

<p><b>OBJECTIVE</b>To establish a method for the determination of hydroxysafflor yellow A in Dedu Honghuaqiwei pill.</p><p><b>METHOD</b>The determination was performed by HPLC method on Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column at 403 nm using methanol-acetonitrile-0.7% phosphoric acid-water (26: 2: 72) as mobile phase. The column temperature was 30 degrees C and the flow rate was 1.0 mL x min(-1).</p><p><b>RESULT</b>The linear rang of hydroxysafflor yellow A was 0.068-0.408 microg and the recovery was 97.66%.</p><p><b>CONCLUSION</b>The result is accurate with good resolution, and the established method can be applied to determine the content of hydroxysafflor yellow A in Dedu Honghuaqiwei pill.</p>


Subject(s)
Chalcone , Chromatography, High Pressure Liquid , Methods , Drugs, Chinese Herbal , Medicine, Mongolian Traditional , Quinones
19.
Article in Chinese | WPRIM | ID: wpr-338061

ABSTRACT

<p><b>OBJECTIVE</b>To establish an UPLC method for simultaneous determination of purpuroxanthine, purpurin, 1,3,6-trihydroxy-2-methylanthraquinone, rubimaillin in carbonized Rubiae Radix et Rhizoma.</p><p><b>METHOD</b>The components were separated on acquity BEHC18 (2.1 mm x 50 mm, 1.7 microm) using methanol and 0.3% formic acid solution as the mobile phase; The flow rate was 0.2 mL x min(-1) and the volume of injection was 2 microL; the column temperature was maintained at 30 degrees C and the detective wavelength was set at 276 nm.</p><p><b>RESULT</b>There were good liner relationships between the peak area and concentration at ranges of 0.68-34.44 mg x L(-1) (r = 0.9999), 0.66-33.2 mg x L(-1) (r = 0.9997), 0.68-34.08 mg x L(-1) (r = 0.9999), 1.07-53.52 mg x L(-1) (r = 0.9999) for purpuroxanthine, purpurin, 1,3,6-trihydroxy-2-methylanthraquinone, rubimaillin, respectively; the average recovery rates of purpuroxanthine, purpurin, 1,3,6-trihydroxy-2-methylanthraquinone, rubimaillin were 96.95%, 95.75%, 102.5%, 96.15%, respectively, with RSD less than 3%.</p><p><b>CONCLUSION</b>The established method was rapid and simple with good accuracy and reproducibility for the determination of carbonized Rubiae Radix et Rhizoma, the method was suitable for the quality control of carbonized Rubiae Radix et Rhizoma.</p>


Subject(s)
Chromatography, High Pressure Liquid , Quinones , Chemistry , Rhizome , Chemistry , Rubia , Chemistry
20.
Article in Chinese | WPRIM | ID: wpr-252944

ABSTRACT

<p><b>OBJECTIVE</b>To develop an HPLC method for determination of gallic acid, hydroxysafflor yellow A, cinnamic aldehyde and piperine in Tibetan medicine Dangzuo, and to compare the content of four active components in Dangzuo of different Tibetan regions.</p><p><b>METHOD</b>The separation was carried out on a Waters XTerra RP-C18 column ( 4.6 mm x 250 mm, 5 microm). The mobile phases were methanol and water, all contained 0.1% glacial acetic acid, for gradient elution. The gradient program was as follows: 0-22.5 min, methanol was changed from 5% to 50%; 22.5-40 min, changed to 80% 80:20. The flow rate was 1.0 mL x min(-1). The detection wavelength was 270 nm. The reference wavelength was 500 nm.</p><p><b>RESULT</b>The linear ranges of gallic acid, hydroxysafflor yellow A, cinnamic aldehyde and piperine were 0.040-0.640 microg (r = 0.999 8), 0.090-1.440 microg (r = 0.999 9), 0.031-0.500 microg (r = 0.999 9 ) and 0.092-41.477 microg (r = 0.998 9), respectively. The average recoveries (n = 6) were 97.42% (RSD 1.9%), 97.55% (RSD 2.9%), 98.69% (RSD 0.96%) and 96.72% (RSD 4.0%), respectively. The content ranges of gallic acid, hydroxysafflor yellow A, cinnamic aldehyde and piperine in Dangzuo samples of different Tibetan regions were 0.11341.69 mg x g(-1), 0.889-1.51 mg x g(-1), 0.000-40.606 mg x g(-1) and 1.96-2.73 mg x g(-1), respectively.</p><p><b>CONCLUSION</b>The method is a simple and effective for quality control of Tibetan medicine Dangzuo.</p>


Subject(s)
Acrolein , Alkaloids , Benzodioxoles , Chalcone , Chromatography, High Pressure Liquid , Methods , Drugs, Chinese Herbal , Chemistry , Gallic Acid , Medicine, Tibetan Traditional , Piperidines , Plant Components, Aerial , Chemistry , Plant Extracts , Polyunsaturated Alkamides , Quality Control , Quinones , Reference Standards , Spectrophotometry, Ultraviolet , Methods
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