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1.
J. nurs. health ; 10(2): 20102009, mai.2020.
Article in Portuguese | LILACS, BDENF | ID: biblio-1104137

ABSTRACT

Objetivo: analisar e categorizar o método de uso e benefícios e principais considerações sobre uso do laser em lesões induzidas por radiação. Método: revisão sistemática desenvolvidas em Public Medline, Literatura Latino-Americana do Caribe em Ciências da Saúde, Cumulative Index to Nursing and Allied Health Literature, SCOPUS e Biblioteca Virtual de Saúde. Resultados: analisou-se sete estudos. Observou-se em 86% (6) desses estudos uma conclusão positiva à terapêutica de laser de baixa intensidade, relatando: 43% (3) diminuição significativa e regressão da dor; 29% (2) redução significativa na duração e gravidade da mucosite e 29% (2) redução da inflamação e cicatrização. Conclusão: evidenciou-se melhora da cicatrização, da dor local e da capacidade anti-inflamatória. É necessário realizar mais pesquisas com amostras robustas que comparem os tipos de lasers, o comprimento das ondas e sua intensidade, para o seu uso como terapêutica.(AU)


Objective: to analyze and categorize the method of use and benefits and main considerations on the use of laser in radiation-induced injuries. Method: systematic review developed in the databases Public Medline, Latin American Caribbean Literature in Health Sciences, Cumulative Index to Nursing and Allied Health Literature, SCOPUS and Virtual Health Library. Results: seven studies were analyzed. A positive conclusion to low-level laser therapy was observed in 86% (6) of these studies, reporting: 43% (3) significant decrease and regression of pain; 29% (2) significant reduction in the duration and severity of mucositis and 29% (2) reduction in inflammation and healing. Conclusion: there was an improvement in healing, local pain and anti-inflammatory capacity. More research is needed with robust samples that compare the types of lasers, the length of the waves and their intensity, for their use as therapy.(AU)


Objetivo: analizar y clasificar el método de uso y los beneficios y las principales consideraciones sobre el uso del láser en lesiones inducidas por radiación. Método: revisión sistemática desarrollada en Public Medline, Literatura Latinoamericana del Caribe en Ciencias de la Salud, Índice acumulativo de literatura de enfermería y salud aliada, SCOPUS y Biblioteca Virtual en Salud. Resultados: se analizaron siete estudios. Se observó una conclusión positiva de la terapia con láser de bajo nivel en el 86% (6) de estos estudios: 43% (3) disminución significativa y regresión del dolor; 29% (2) reducción significativa en la duración y gravedad de la mucositis y 29% (2) reducción en la inflamación y la curación. Conclusión: hubo una mejora en la curación, dolor local y capacidad antiinflamatoria. Se necesita investigación con muestras robustas que comparen los tipos de láser, la longitud de las ondas y su intensidad, para su uso como terapia.(AU)


Subject(s)
Radiation Effects , Radiodermatitis , Radiotherapy , Low-Level Light Therapy
2.
Article in English | WPRIM | ID: wpr-828990

ABSTRACT

Objective@#Owing to antibiotic abuse and the subsequent development of antibiotic resistance, bacterial infection has become one of the most persistent unresolved problems. New antibacterial agents, especially those that are environmental-friendly, are urgently needed.@*Methods@#Melanin extracted by filtration centrifugation and acid and proteolytic hydrolysis was characterized using UV, FTIR, TEM, and XPS. Photothermal conversion was calculated, and the bacteriostatic effects, and , were assessed by plate counting and ratios (%) of wound areas.@*Results@#Natural melanin hydrolyzed by trypsin had good photothermal conversion effects, which resulted in superior bacteriostatic activities. The extracted melanin along with laser NIR irradiation at 808 nm promoted the healing of wounds infected by drug-resistant bacteria and was biocompatible according to toxicity tests and .@*Conclusion@#The present findings indicated a safe and efficient method of developing natural antibacterial agents.


Subject(s)
Animal Shells , Chemistry , Animals , Anti-Bacterial Agents , Pharmacology , Escherichia coli , Radiation Effects , Escherichia coli Infections , Drug Therapy , Melanins , Pharmacology , Mytilus edulis , Chemistry , Photochemical Processes , Rats , Rats, Sprague-Dawley , Staphylococcal Infections , Drug Therapy , Staphylococcus aureus , Radiation Effects , Wound Healing
3.
Article in English | WPRIM | ID: wpr-828975

ABSTRACT

Objective@#This study aimed to investigate the effect of exposure to a 900 MHz electromagnetic field (EMF) on the cervical spinal cord (CSC) of rats and the possible protective effect of luteolin (LUT) against CSC tissue damage.@*Methods@#Quantitative data were obtained stereological, biochemical, immunohistochemical, and histopathological techniques. We investigated morphometric value, superoxide dismutase (SOD) level, and the expression of high-mobility group box 1 protein molecules, as well as histological changes.@*Results@#The total number of motor neurons in the EMF group significantly decreased in comparison with that in the control group ( < 0.05). In the EMF + LUT group, we found a significant increase in the total number of motor neurons compared with that in the EMF group ( < 0.05). SOD enzyme activity in the EMF group significantly increased in comparison with that in the control group ( < 0.05). By contrast, the EMF+LUT group exhibited a decrease in SOD level compared with the EMF group ( < 0.05).@*Conclusion@#Our results suggested that exposure to EMF could be deleterious to CSC tissues. Furthermore, the protective efficacy of LUT against SC damage might have resulted from the alleviation of oxidative stress caused by EMF.


Subject(s)
Animals , Antioxidants , Pharmacology , Electromagnetic Fields , Luteolin , Pharmacology , Male , Rats , Rats, Wistar , Spinal Cord , Radiation Effects
4.
Article in English | WPRIM | ID: wpr-828974

ABSTRACT

Objective@#To detect the effects of shortwave radiation on dose-dependent cardiac structure and function in rats after radiation and to elucidate the mechanism of shortwave radiation induced cardiac injury to identify sensitive indicators and prophylactic treatment.@*Methods@#One hundred Wistar rats were either exposed to 27 MHz continuous shortwave at a power density of 5, 10, and 30 mW/cm for 6 min or undergone sham exposure for the control (the rats had to be placed in the exposure system with the same schedules as the exposed animals, but with an inactive antenna). The Ca , glutamic oxaloacetic transaminase (AST), creatine kinase (CK) and lactate dehydrogenase (LDH) content in the peripheral serum of the rats were detected by an automatic blood biochemical analyser. The electrocardiogram (ECG) of standard lead II was recorded by a multi-channel physiological recording and analysis system. The cardiac structure of rats was observed by light and electron microscopy.@*Results@#The results showed that the 5, 10, and 30 mW/cm shortwave radiation caused a significant increased in the levels of Ca , AST, CK, and LDH in the peripheral serum of rats. The cardiac structure was damaged by radiation and showed a disordered arrangement of myocardial fibres, the cavitation and swelling of myocardial mitochondria. These injuries were most significant 7 d after radiation and were not restored until 28 d after radiation.@*Conclusion@#Shortwave radiation of 5, 10, and 30 mW/cm can damage rat cardiac function, including damage to the tissue structure and ultrastructure, especially at the level of the myocardial fibres and mitochondria. Shortwave radiation at 5, 10, and 30 mW/cm induced damage to rat heart function and structure with a dose-effect relationship, i.e., the greater the radiation dose was, the more significant the damage was.


Subject(s)
Animals , Dose-Response Relationship, Radiation , Heart , Radiation Effects , Heart Diseases , Ethnology , Pathology , Male , Myocardium , Pathology , Radio Waves , Random Allocation , Rats , Rats, Wistar
5.
Article in English | WPRIM | ID: wpr-828959

ABSTRACT

Radiotherapy is one of the most common treatments for oral cancer. However, in the clinic, recurrence and metastasis of oral cancer occur after radiotherapy, and the underlying mechanism remains unclear. Cancer stem cells (CSCs), considered the "seeds" of cancer, have been confirmed to be in a quiescent state in most established tumours, with their innate radioresistance helping them survive more easily when exposed to radiation than differentiated cancer cells. There is increasing evidence that CSCs play an important role in recurrence and metastasis post-radiotherapy in many cancers. However, little is known about how oral CSCs cause tumour recurrence and metastasis post-radiotherapy. In this review article, we will first summarise methods for the identification of oral CSCs and then focus on the characteristics of a CSC subpopulation induced by radiation, hereafter referred to as "awakened" CSCs, to highlight their response to radiotherapy and potential role in tumour recurrence and metastasis post-radiotherapy as well as potential therapeutics targeting CSCs. In addition, we explore potential therapeutic strategies targeting these "awakened" CSCs to solve the serious clinical challenges of recurrence and metastasis in oral cancer after radiotherapy.


Subject(s)
Humans , Mouth Neoplasms , Pathology , Radiotherapy , Neoplasm Recurrence, Local , Radiotherapy , Neoplastic Stem Cells , Pathology , Radiation Effects , Radiotherapy , Methods , Recurrence
6.
Article in Chinese | WPRIM | ID: wpr-772334

ABSTRACT

The lung is the second most common site of neuroendocrine tumors (NETs). Typical and atypical carcinoids are low-grade NETs of the lung. These rare tumors have received little attention and education is needed for treating physicians. The article describes the classifcation of lung NETs, the epidemiology and pathological characteristics. When lung NETs are diagnosed at an early stage, surgical intervention is often curative. For advanced lung NETs patients, different treatment methods including chemotherapy, somatostatin analogs, m-TOR inhibition, peptide receptor radioligand therapy, and biologic systemic therapy are discussed. The conclusions are generally extrapolated from the outcome of extra-pulmonary carcinoids. Prospective randomized well-designed trials are urgently needed to inform current recommendations on systemic treatment.
.


Subject(s)
Disease-Free Survival , Drug Therapy , Methods , Humans , Lung , Radiation Effects , General Surgery , Lung Neoplasms , Pathology , General Surgery , Therapeutics , Neoplasm Grading , Neuroendocrine Tumors , Pathology , General Surgery , Therapeutics , Outcome Assessment, Health Care , Radiotherapy , Methods
7.
Article in English | WPRIM | ID: wpr-765360

ABSTRACT

OBJECTIVE: We retrospectively assessed the efficacy of stereotactic radiosurgery (SRS) for dural arteriovenous fistulas (DAVFs) involving the transverse-sigmoid sinus and analyzed the angiographic and clinical results with our 8-year experience. METHODS: Nine patients with intracranial DAVFs involving the transverse-sigmoid sinus underwent SRS using a Gamma Knife® (Elekta Inc., Atlanta, GA, USA) between 2009 and 2016. Five patients underwent SRS for residual DAVFs after embolization and four patients were treated with SRS alone. The median target volume was 1.9 cm3 (range, 0.8–14.2) and the median radiation dose of the target was 17 Gy (range, 16–20). The median follow-up period was 37 months (range, 7–81). RESULTS: Pulsating tinnitus (33%) was the most common symptom. DAVFs were completely obliterated in four patients (44%) and subtotally obliterated in five (56%). Six patients (67%) showed complete recovery of symptoms or signs, and three (33%) showed incomplete recovery. One patient experienced a recurrent seizure. Adverse radiation effects after SRS occurred in one patient (11%). The total obliteration rates after SRS were 16.7%, 37.5%, and 68.7% at 1, 2, and 3 years, respectively. The median interval from SRS to total obliteration of the fistula was 31 months (range, 12–38). The rates at which the symptoms started to improve were 40% at 1 month and 80% at 2 months after SRS. Symptoms started to improve at a median of 5 weeks after SRS (range, 3–21). CONCLUSION: SRS with or without embolization is a safe and effective treatment to relieve symptoms and obliterate DAVFs on the transverse-sigmoid sinus.


Subject(s)
Central Nervous System Vascular Malformations , Fistula , Follow-Up Studies , Humans , Radiation Effects , Radiosurgery , Retrospective Studies , Seizures , Tinnitus , Transverse Sinuses
8.
Article in Chinese | WPRIM | ID: wpr-775240

ABSTRACT

OBJECTIVE@#To investigate the effect of low-frequency pulsed electromagnetic fields (PEMF) on the maturation and mineralization of rat cranial osteoblasts and its relation to IGF-1R/NO signaling pathway.@*METHODS@#The rat osteoblasts were isolated and cultured and randomly divided into blank control group, PEMF group, GSK group (IGF-1R blocker) and PEMF+GSK group. The cells were treated with 50 Hz 0.6 mT PEMF for 1.5 h/d. After 3 d of PEMF treatment, the expressions of protein kinase (AKT), inducible nitric oxide synthase (iNOS) and cGMP-dependent protein kinase (PKG) were detected by Western blotting; on 6 d of PEMF treatment alkaline phosphatase (ALP) activity was determined; on 12 d of PEMF treatment the calcification nodule formation was demonstrated by Alizarin red staining.@*RESULTS@#NO level was significantly increased in rat osteoblasts treated with 50 Hz 0.6 mT PEMF for 1.5 h/d. Western blot analysis showed that the expressions of AKT, iNOS and PKG protein in PEMF group were higher than those in the control group (all <0.01); the ALP activity was increased(<0.05), and the PEMF group had the largest area of Alizarin red staining (<0.01). The expressions of AKT, iNOS and PKG protein in GSK group were lower than those in the control group; the ALP activity was decreased (<0.05), and the GSK group had the least area of Alizarin red staining (<0.01). The expressions of AKT, iNOS, PKG protein, the ALP activity and the area of Alizarin red staining in PEMF+GSK group were between PEMF group and GSK group.@*CONCLUSIONS@#PEMF may enhance the maturation and mineralization of rat cranial osteoblasts through IGF-1R/NO signaling pathway.


Subject(s)
Animals , Cell Differentiation , Cell Proliferation , Cells, Cultured , Electromagnetic Fields , Nitric Oxide , Metabolism , Osteoblasts , Radiation Effects , Rats , Receptor, IGF Type 1 , Metabolism , Signal Transduction , Radiation Effects
9.
Article in English | WPRIM | ID: wpr-788787

ABSTRACT

OBJECTIVE: We retrospectively assessed the efficacy of stereotactic radiosurgery (SRS) for dural arteriovenous fistulas (DAVFs) involving the transverse-sigmoid sinus and analyzed the angiographic and clinical results with our 8-year experience.METHODS: Nine patients with intracranial DAVFs involving the transverse-sigmoid sinus underwent SRS using a Gamma Knife® (Elekta Inc., Atlanta, GA, USA) between 2009 and 2016. Five patients underwent SRS for residual DAVFs after embolization and four patients were treated with SRS alone. The median target volume was 1.9 cm3 (range, 0.8–14.2) and the median radiation dose of the target was 17 Gy (range, 16–20). The median follow-up period was 37 months (range, 7–81).RESULTS: Pulsating tinnitus (33%) was the most common symptom. DAVFs were completely obliterated in four patients (44%) and subtotally obliterated in five (56%). Six patients (67%) showed complete recovery of symptoms or signs, and three (33%) showed incomplete recovery. One patient experienced a recurrent seizure. Adverse radiation effects after SRS occurred in one patient (11%). The total obliteration rates after SRS were 16.7%, 37.5%, and 68.7% at 1, 2, and 3 years, respectively. The median interval from SRS to total obliteration of the fistula was 31 months (range, 12–38). The rates at which the symptoms started to improve were 40% at 1 month and 80% at 2 months after SRS. Symptoms started to improve at a median of 5 weeks after SRS (range, 3–21).CONCLUSION: SRS with or without embolization is a safe and effective treatment to relieve symptoms and obliterate DAVFs on the transverse-sigmoid sinus.


Subject(s)
Central Nervous System Vascular Malformations , Fistula , Follow-Up Studies , Humans , Radiation Effects , Radiosurgery , Retrospective Studies , Seizures , Tinnitus , Transverse Sinuses
10.
Article in English | WPRIM | ID: wpr-773419

ABSTRACT

OBJECTIVE@#Pyroptosis is an inflammatory form of programmed cell death. This phenomenon has been recently reported to play an important role in radiation-induced normal tissue injury. Connexin43 (Cx43) is a gap junction protein that regulates cell growth and apoptosis. In this study, we investigated the effect of Cx43 on X-ray-induced pyroptosis in the human umbilical vein endothelial cells (HUVECs).@*METHODS@#HUVECs, Cx43 overexpression, and Cx43 knockdown strains were irradiated with 10 Gy. Proteins were detected using western blot analysis. Cell pyroptosis was evaluated using the fluorescence-labeled inhibitor of caspase assay (FLICA) and propidium iodide staining through flow cytometry and confocal microscopy. Cell morphology and cytotoxicity were detected by scanning electron microscopy and lactate dehydrogenase release assay, respectively.@*RESULTS@#Irradiation with 10 Gy X-ray induced pyroptosis in the HUVECs and reduced Cx43 expression. The pyroptosis in the HUVECs was significantly attenuated by overexpression of Cx43 as it decreased the level of active caspase-1. However, interference of Cx43 expression with siRNA significantly promoted pyroptosis by increasing the active caspase-1 level. Pannexin1 (Panx1), a gap junction protein regulates pyroptosis, and its cleaved form is used to evaluate channel opening and active state. The level of cleaved Panx1 in the HUVECs and Cx43 knockdown strains increased in the presence of X-ray, but decreased in the Cx43 overexpression strains. Furthermore, interference of Panx1 with siRNA alleviated the upregulation of pyroptosis caused by Cx43 knockdown.@*CONCLUSION@#Results suggest that single high-dose X-ray irradiation induces pyroptosis in the HUVECs. In addition, Cx43 regulates pyroptosis directly by activating caspase-1 or indirectly by cleaving Panx1.


Subject(s)
Caspase 1 , Genetics , Metabolism , Connexin 43 , Genetics , Metabolism , Connexins , Genetics , Metabolism , Gene Expression Regulation , Radiation Effects , Human Umbilical Vein Endothelial Cells , Physiology , Radiation Effects , Humans , Nerve Tissue Proteins , Genetics , Metabolism , Pyroptosis , X-Rays
11.
Article in English | WPRIM | ID: wpr-773418

ABSTRACT

OBJECTIVE@#To estimate the detrimental effects of shortwave exposure on rat hippocampal structure and function and explore the underlying mechanisms.@*METHODS@#One hundred Wistar rats were randomly divided into four groups (25 rats per group) and exposed to 27 MHz continuous shortwave at a power density of 5, 10, or 30 mW/cm2 for 6 min once only or underwent sham exposure for the control. The spatial learning and memory, electroencephalogram (EEG), hippocampal structure and Nissl bodies were analysed. Furthermore, the expressions of N-methyl-D-aspartate receptor (NMDAR) subunits (NR1, NR2A, and NR2B), cAMP responsive element-binding protein (CREB) and phosphorylated CREB (p-CREB) in hippocampal tissue were analysed on 1, 7, and 14 days after exposure.@*RESULTS@#The rats in the 10 and 30 mW/cm2 groups had poor learning and memory, disrupted EEG oscillations, and injured hippocampal structures, including hippocampal neurons degeneration, mitochondria cavitation and blood capillaries swelling. The Nissl body content was also reduced in the exposure groups. Moreover, the hippocampal tissue in the 30 mW/cm2 group had increased expressions of NR2A and NR2B and decreased levels of CREB and p-CREB.@*CONCLUSION@#Shortwave exposure (27 MHz, with an average power density of 10 and 30 mW/cm2) impaired rats' spatial learning and memory and caused a series of dose-dependent pathophysiological changes. Moreover, NMDAR-related CREB pathway suppression might be involved in shortwave-induced structural and functional impairments in the rat hippocampus.


Subject(s)
Animals , Cyclic AMP Response Element-Binding Protein , Genetics , Metabolism , Dose-Response Relationship, Radiation , Electroencephalography , Radiation Effects , Hippocampus , Radiation Effects , Male , Memory , Radiation Effects , Nissl Bodies , Physiology , Radiation Effects , Radio Waves , Random Allocation , Rats , Rats, Wistar , Receptors, N-Methyl-D-Aspartate , Genetics , Metabolism , Spatial Learning , Radiation Effects
12.
Article in Chinese | WPRIM | ID: wpr-773263

ABSTRACT

Chlorophyll content,leaf mass to per area,net photosynthetic rate and bioactive ingredients of Asarum heterotropoides var. mandshuricum,a skiophyte grown in four levels of solar irradiance were measured and analyzed in order to investigate the response of photosynthetic capability to light irradiance and other environmental factors. It suggested that the leaf mass to per area of plant was greatest value of four kinds of light irradiance and decreasing intensity of solar irradiance resulted in the decrease of leaf mass to per area at every phenological stage. At expanding leaf stage,the rate of Chla and Chlb was 3. 11 when A. heterotropoides var. mandshuricum grew in full light irradiance which is similar to the rate of heliophytes,however,the rate of Chla and Chlb was below to 3. 0 when they grew in shading environment. The content of Chla,Chlb and Chl( a+b) was the greatest value of four kinds of light irradiance and decreasing intensity of solar irradiance resulted in its decreasing remarkably( P<0. 05). The rate of Chla and Chlb decreased but the content of Chla,Chlb and Chl( a+b) increased gradually with continued shading. The maximum value of photosynthetically active radiation appeared at 10: 00-12: 00 am in a day. The maximum value of net photosynthetic rate appeared at 8: 30-9: 00 am and the minimum value appeared at 14: 00-14: 30 pm at each phenological stage if plants grew in full sunlight. However,when plants grew in shading,the maximum value of net photosynthetic rate appeared at about 10: 30 am and the minimum value appeared at 12: 20-12: 50 pm at each phenological stage. At expanding leaf stage and flowering stage,the average of net photosynthetic rate of leaves in full sunlight was remarkably higher than those in shading and it decreased greatly with decreasing of irradiance gradually( P < 0. 05). However,at fruiting stage,the average of net photosynthetic rate of leaves in full sunlight was lower than those in 50% and 28% full sunlight but higher than those in 12% full sunlight. All photosynthetic diurnal variation parameters of plants measured in four kinds of different irradiance at three stages were used in correlation analysis. The results suggested that no significant correlation was observed between net photosynthetic rate and photosynthetically active radiation,and significant negative correlation was observed between net photosynthetic rate and environmental temperature as well as vapor pressure deficit expect for 12% full sunlight. Positive correlation was observed between net photosynthestic rate and relative humidity expect for 12% full sunlight. Significant positive correlation was observed between net photosynthetic rate and stomatal conductance in the four light treatments. Only,in 12% full sunlight,the net photosynthetic rate was significantly related to photosynthetically active radiation rather than related to environmental temperature,vapor pressure deficit and relative humidity. In each light treatment,a significant positive correlation was observed between environmental temperature and vapor pressure deficit,relative humidity as well as stomatal conductance. Volatile oil content was 1. 46%,2. 16%,1. 56%,1. 30% respectively. ethanol extracts was 23. 44%,22. 45%,22. 18%,21. 12% respectively. Asarinin content was 0. 281%,0. 291%,0. 279% and 0. 252% respectively. The characteristic components of Asarum volatile oil of plant in different light treatments did not change significantly among different groups.


Subject(s)
Asarum , Physiology , Radiation Effects , Chlorophyll , Photosynthesis , Plant Leaves , Radiation Effects , Sunlight
13.
Article in Chinese | WPRIM | ID: wpr-773241

ABSTRACT

We studied the seed germination of Astragalus membranaceus under PEG and Na Cl osmotic stress gradients( 0,-0. 1,-0. 3,-0. 5,-0. 7 MPa) respectively applied with light( continuous light,light 12 h/dark 12 h circulation and continuous dark) and temperature( constant 15 ℃,15 ℃ 12 h/30 ℃ 12 h circulation and constant 30 ℃) treatments. The results showed as following: ① Under the light and temperature interactive treatments,total germination percentage( TGP) was restrained by high temperature and continuous light also decreased TGP under high temperature. Mean germination time( MGT) was not changed by light mode. Root development was enhanced by dark and low temperature. Shoot development was enhanced by light and high temperature. Hypocotyl length was enhanced by dark and high temperature. ② Under the light and temperature interactive treatments combined respectively with PEG and NaCl stress conditions,although the inhibitions of seed germination and growth were gradually strengthened with the increases of osmotic stresses,slight osmotic stress can promote seed germination. Under the same osmotic potential,the effects of PEG on TGPs and MGTs were stronger than that of NaCl. As the temperature increase,the seeds may change from photo-neutrality to photo-phobia. Decreased TGP under drought and continuous light interactive treatment is an adaptation strategy to avoiding drought. Hypocotyl growth accelerated under continuous dark treatment is an ecological trait which could increase dry matter input in stem and height for more light. Seed development under high concentration of NaCl treatment is better than that of PEG treatment due to low water potential caused by Na~+,which can enter into seed coat and promote water absorption.


Subject(s)
Astragalus propinquus , Physiology , Radiation Effects , Droughts , Germination , Light , Salt Stress , Seeds , Physiology , Radiation Effects , Temperature
14.
Acta Physiologica Sinica ; (6): 388-394, 2019.
Article in Chinese | WPRIM | ID: wpr-777175

ABSTRACT

The aim of this study was to determine the effects of extremely low frequency electromagnetic field (ELF-EMF) on energy metabolism and oxidative stress in Caenorhabditis elegans (C. elegans). Worms in three adult stages (young adult stage, egg-laying stage and peak egg-laying stage) were investigated under 50 Hz, 3 mT ELF-EMF exposure. ATP levels, ATP synthase activity in vivo, reactive oxygen species (ROS) content, and changes of total antioxidant capacity (TAC) were detected, and worms' oxidative stress responses were also evaluated under ELF-EMF exposure. The results showed that ATP levels were significantly increased under this ELF-EMF exposure, and mitochondrial ATP synthase activity was upregulated simultaneously. In young adult stage, worms' ROS level was significantly elevated, together with upregulated TAC but with a decreased ROS-TAC score indicated by principal component analysis. ROS level and TAC of worms had no significant changes in egg-laying and peak egg-laying stages. Based on these results, we concluded that ELF-EMF can enhance worm energy metabolism and elicit oxidative stress, mainly manifesting as ATP and ROS level elevation together with ATP synthase upregulation and ROS-TAC score decrease in young adult C. elegans.


Subject(s)
Adenosine Triphosphate , Metabolism , Animals , Caenorhabditis elegans , Radiation Effects , Electromagnetic Radiation , Energy Metabolism , Mitochondrial Proton-Translocating ATPases , Metabolism , Oxidative Stress , Reactive Oxygen Species
15.
Acta Physiologica Sinica ; (6): 431-438, 2019.
Article in Chinese | WPRIM | ID: wpr-777170

ABSTRACT

The present study was aimed to investigate the effects and mechanisms of electro-acupuncture (EA) on proliferation and differentiation of neural stem cells in the hippocampus of C57 mice exposed to different doses of X-ray radiation. Thirty-day-old C57BL/6J mice were randomly divided into control, irradiation, and EA groups. The control group was not treated with irradiation. The irradiation groups were exposed to different doses of X-ray (4, 8 or 16 Gy) for 10 min. The EA groups were electro-acupunctured at Baihui, Fengfu and bilateral Shenyu for 3 courses of treatment after X-ray radiation. Immunohistochemistry was used to evaluate proliferation and differentiation of the hippocampal neural stem cell. RT-PCR and Western blot were used to detect mRNA and protein expressions of Notch1 and Mash1 in the hippocampus, respectively. The results showed that, compared with the control group, the numbers of BrdU positive cells (4, 8 Gy subgroup) and BrdU/NeuN double-labeling positive cells (3 dose subgroups) were decreased significantly in the irradiation group, but the above changes could be reversed by EA. Compared with the control group, the number of BrdU/GFAP double-labeling positive cells in each dose subgroup of irradiation group was decreased significantly, while EA could reverse the change of 4 and 8 Gy dose subgroups. In addition, compared with the control group, the expression levels of Notch1 mRNA and protein in hippocampus were up-regulated, and the expression levels of Mash1 mRNA and protein were significantly decreased in each dose subgroup of irradiation group. Compared with irradiation group, the expression levels of Notch1 mRNA and protein in hippocampus of EA group were decreased significantly in each dose subgroup, and the expression levels of Mash1 mRNA and protein were increased significantly in 4 and 8 Gy subgroups. These results suggest that irradiation affects the proliferation and differentiation of neural stem cells in hippocampus of mice, whereas EA may significantly increase the proliferation and differentiation of hippocampal neural stem cells via the regulation of Notch signaling pathway.


Subject(s)
Animals , Basic Helix-Loop-Helix Transcription Factors , Metabolism , Cell Differentiation , Cell Proliferation , Electroacupuncture , Hippocampus , Cell Biology , Radiation Effects , Mice, Inbred C57BL , Neural Stem Cells , Cell Biology , Radiation Effects , Random Allocation , Receptor, Notch1 , Metabolism , X-Rays
16.
Acta Physiologica Sinica ; (6): 698-704, 2019.
Article in Chinese | WPRIM | ID: wpr-777141

ABSTRACT

The aim of this study was to investigate the relationship between the effects of different doses of X-rays on DNA damage and JAK/STAT signaling pathway activation in A549 cells. The A549 cells were radiated with X-rays at doses of 2, 4, and 8 Gy. The proliferation of A549 cells was detected by CCK8 method. The content of interleukin 6 (IL-6) in culture medium at different time points after irradiation was detected by enzyme-linked immunoassay, and the expression levels of IL-6 receptor (IL-6R) and p53 binding protein 1 (53BP1) were detected by immunofluorescent staining. The expression levels of JAK2, p-JAK2, STAT3 and p-STAT3 were detected by Western blot. The results showed that, compared with the control group, X-ray irradiation reduced the cellular proliferation, up-regulated the expression of 53BP1, increased the IL-6 content in the medium supernatant, and up-regulated the protein expression levels of IL-6R, JAK2, p-JAK2, STAT3, and p-STAT3. The above effects of X-ray irradiation were dose-dependent. These results suggest that the mechanism by which X-rays cause DNA damage in A549 cells may involve activation of the JAK/STAT signaling pathway.


Subject(s)
A549 Cells , DNA Damage , Radiation Effects , Humans , Janus Kinase 2 , Metabolism , Receptors, Interleukin-6 , Metabolism , STAT3 Transcription Factor , Metabolism , Signal Transduction , Tumor Suppressor p53-Binding Protein 1 , Metabolism , X-Rays
17.
Article in Chinese | WPRIM | ID: wpr-774161

ABSTRACT

The deoxyribonucleic acid (DNA) molecule damage simulations with an atom level geometric model use the traversal algorithm that has the disadvantages of quite time-consuming, slow convergence and high-performance computer requirement. Therefore, this work presents a density-based spatial clustering of applications with noise (DBSCAN) clustering algorithm based on the spatial distributions of energy depositions and hydroxyl radicals (·OH). The algorithm with probability and statistics can quickly get the DNA strand break yields and help to study the variation pattern of the clustered DNA damage. Firstly, we simulated the transportation of protons and secondary particles through the nucleus, as well as the ionization and excitation of water molecules by using Geant4-DNA that is the Monte Carlo simulation toolkit for radiobiology, and got the distributions of energy depositions and hydroxyl radicals. Then we used the damage probability functions to get the spatial distribution dataset of DNA damage points in a simplified geometric model. The DBSCAN clustering algorithm based on damage points density was used to determine the single-strand break (SSB) yield and double-strand break (DSB) yield. Finally, we analyzed the DNA strand break yield variation trend with particle linear energy transfer (LET) and summarized the variation pattern of damage clusters. The simulation results show that the new algorithm has a faster simulation speed than the traversal algorithm and a good precision result. The simulation results have consistency when compared to other experiments and simulations. This work achieves more precise information on clustered DNA damage induced by proton radiation at the molecular level with high speed, so that it provides an essential and powerful research method for the study of radiation biological damage mechanism.


Subject(s)
Algorithms , Computer Simulation , DNA , Radiation Effects , DNA Damage , Linear Energy Transfer , Monte Carlo Method , Protons
18.
Article in English | WPRIM | ID: wpr-771436

ABSTRACT

OBJECTIVE@#To investigate the effect of a modified Wuzi Yanzong Pill (, WZYZP) on the male rats' testis after microwave radiation, as well as its potential mechanism.@*METHODS@#Forty-five male rats were randomly assigned to three groups: the control group, the radiation group, and the WZYZP group. The rats in the radiation group and WZYZP group were exposed to microwave radiation for 15 min once, while the rats in the control group were not exposed to any radiation. The rats in the WZYZP group were given a modified of WZYZP by gavage daily for 7 days. Apoptosis in the testis was evaluated using terminal-deoxynucleoitidyl transferase mediated nick end labeling (TUNEL) assay. Histopathological alterations of the testis were observed by haematoxylin-eosin (HE) staining. Tat-interactive protein, 60kD (Tip60) and p53 expressions were determined by Western blotting.@*RESULTS@#The apoptosis index (AI) in the radiation group was higher than that of the WZYZP group and control group on day 1 (D1), day 7 (D7) day 14 (D14) after radiation (P<0.05). The seminiferous tubules were of normal morphology in the control group. In the radiation group, the partial seminiferous tubules were collapsed, basement membranes of the seminiferous epithelia became detached. WZYZP could restore the morphological changes. There was no expression of Tip60 among the three groups on D7 and D14. The expression of p53 was higher in the radiation group than in the control group (P<0.05). WZYZP could down-regulate the rising p53 induced by radiation on D7 and D14 (P<0.05).@*CONCLUSION@#A modified WZYZP may affect germ cells, and its protective effects may partly result from its ability to intervene in Tip60 mediated apoptosis.


Subject(s)
Animals , Apoptosis , Drugs, Chinese Herbal , Pharmacology , Male , Microwaves , Rats, Wistar , Testis , Metabolism , Pathology , Radiation Effects , Trans-Activators , Metabolism , Tumor Suppressor Protein p53 , Metabolism
19.
Rev. luna azul ; 49(0): 200-219, 2019. tab, ilus
Article in Spanish | LILACS | ID: biblio-1121056

ABSTRACT

Introducción. Este trabajo de investigación se realizó en la Ciudad de Honda, Colombia. Identificó el efecto de la sombra de los árboles en la atenuación de la temperatura, humedad relativa, temperatura de superficie del suelo, radiación solar y ultravioleta (UV) y determinó requerimientos de agua para la vegetación arbórea. Metodología. Se tomaron datos de temperatura del aire y de superficie de suelo, humedad relativa del aire, radiaciones solares y UV bajo la sombra de árboles y a plena exposición. Resultados. Se encontró que la temperatura del aire osciló entre 50ºC a plena exposición solar y 41ºC bajo sombra. La temperatura de superficie presentó a plena exposición solar valores de 66.8ºC y 42.6ºC bajo sombra. La variación de la radiación solar registrada ofreció un valor de 17.13 mW/cm2 a plena exposición solar y 1.69 mW/cm2 bajo sombra. La radiación UV presentó valores de 8 a plena exposición solar y 5 bajo sombra. La demanda hídrica por planta/día varió entre 5 y más de 500 litros, siendo la demanda diaria cercana a 2500 m3 . Conclusiones. En la ciudad no se realiza riego a los árboles, lo que conduce a que se produzca un déficit hídrico evidenciado por la pérdida de follaje ocasionando mínimas atenuaciones a la radiación UV. Las especies más relevantes respecto a la sombra son almendro (Terminalia cattapa), Pallandé (Pitecellobium dulce), naranjuelo (Capparis odoratissima), guayacán carrapo (Bulnesia carrapo), chirlobirlo (Tecoma stands), cumulá (Aspidosperma polyneuron) y mango (Manguifera indica). Los árboles pueden generar un buen servicio ecosistémico por la sombra, este servicio está mediado por la selección de la especie y el manejo de que son objeto los árboles.


Introduction: This research work was carried out in the city of Honda, Colombia. It identified the effect of tree shade on temperature attenuation, relative humidity, soil surface temperature, solar and ultraviolet (UV) radiation, and determined water requirements for arboreal vegetation. Methodology: Data on air and soil surface temperature, relative air humidity, solar and UV radiation were taken under the shade of trees and at full exposure. Results: It was found that the air temperature ranged from 50ºC at full sun exposure to 41ºC under shade. The surface temperature was 66.8ºC and 42.6ºC under shade. The variation of the registered solar radiation offered a value of 17.13 mW/cm2 at full solar exposure and 1.69 mW/cm2 under shade. The UV radiation presented values of 8 at full solar exposure and 5 under shade. The water demand per plant/day varied between 5 and more than 500 liters, being the daily demand close to 2500 m3. Conclusions: There is no irrigation of trees in the city which leads to a water deficit evidenced by the loss of foliage causing minimal attenuations to UV radiation. The most relevant species regarding shade are almond (Terminalia cattapa), Pallandé (Pitecellobium dulce), orange (Capparis odoratissima), guayacán carrapo (Bulnesia carrapo), chirlobirlo (Tecoma stands), cumula (Aspidosperma polyneuron) and mango (Manguifera indica). Trees can generate a good ecosystem service by shade which is mediated by the selection of the species and the management of the trees.


Subject(s)
Humans , Cities , Radiation Effects , Urban Area , Ecology
20.
Rev. méd. panacea ; 7(3): 97-100, sept.-dic. 2018.
Article in Spanish | LILACS | ID: biblio-1016897

ABSTRACT

Objetivo: Identificar la producción científica sobre nivel de conocimientos, actitudes y prácticas sobre fotoprotección en padres de menores de 14 años latinoamericanos. Materiales y métodos:Estudio bibliométrico descriptivo y transversal. Se realizó una búsqueda bibliográfica en las bases de datos: PubMed, Scopus y la Biblioteca Virtual de Salud, utilizando las palabras claves: "fotoprotección" "niños", "escolares", entre el primero de enero del 2013 al 31 de junio del 2018. Se excluyeron artículos no originales o que no midieran conocimiento sobre fotoprotección. Resultado: Se encontró un total de seis artículos el 28.3% de los padres reportaron que sus hijos se exponen más de 60 minutos al sol entre semana; en el fin de semana se incrementa a un 42.8%. En Chile, el 14.2% de los niños han presentado alguna vez quemadura solar, con 2/3 de la población de estudio pasan más de 2 horas diarias al exterior. En Uruguay, El 55% de los padres afirmó que se protegen del sol, el 30% solo a veces y el 15% no se protege. En Cuba el 72.5% han presentado al menos una quemadura solar. En Perú, El estudio peruano demostró que el 48% utiliza protector solar en crema, el 46% ropa de protección y el 51% gorro o sombrero. Conclusiones: Se requieren intervenciones sanitarias para incrementar el conocimiento de los padres sobre fotoprotección, en México demuestra que una intervención educativa puede incrementar en un futuro el conocimiento de los jóvenes en el cuidado de la piel. (AU)


Objective: To identify scientific production on the level of knowledge, attitudes, and practices on photoprotection in parents of children under 14 years of age in Latin America. Materials and methods: Descriptive and transversal bibliometric study. A bibliographic search was carried out in the databases: PubMed, Scopus and the Virtual Health Library, using the keywords: "photoprotection" "children", "school children", between January 1, 2013, and June 31, 2018, We excluded articles that were not original or that did not measure knowledge about photoprotection. Result: A total of six articles were found, 28.3% of parents reported that their children are exposed to more than 60 minutes in the sun during the week; in the weekend it increases to 42.8%. In Chile, 14.2% of children have ever presented sunburn, with 2/3 of the study population spending more than 2 hours a day abroad. In Uruguay, 55% of parents said they protect themselves from the sun, 30% only sometimes and 15% do not protect themselves. In Cuba, 72.5% have presented at least one sunburn. In Peru, the Peruvian study showed that 48% use sunscreen cream, 46% protective clothing and 51% hat or hat. Conclusions: Health interventions are required to increase parents' knowledge about photoprotection, in Mexico it shows that an educational intervention can increase the knowledge of young people in skin care in the future. (AU)


Subject(s)
Humans , Male , Female , Radiation Effects , Health Knowledge, Attitudes, Practice , Sunbathing , Skin Cream , Epidemiology, Descriptive , Cross-Sectional Studies
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