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1.
Biosci. j. (Online) ; 36(1): 245-255, jan./feb. 2020. tab, ilus
Article in English | LILACS (Americas) | ID: biblio-1049246

ABSTRACT

Paracetamol (PCM) overdose can cause hepatotoxicity with oxidative stress; the present study was carried out to establish the possible protective effect of olive leaves extract (OLE) on toxicity induced by paracetamol in adult male rats. Twenty four adult male rats were divided into four equal groups; control, olive leaves extract group, paracetamol group and olive leaves extract plus paracetamol group. Some biochemical parameters and liver histopathology were evaluated. PCM treatment significantly increased serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin, gamma-glutamyltransferase (GGT), lactate dehydrogenase (LDH), urea, creatinine and alpha-fetoprotein. Paracetamol was found to significantly increase malonaldehyde (MDA) and decrease glutathione reductase (GR) activity in tissue and significantly decrease total antioxidant capacity (TAC) and superoxide dismutase (SOD) in serum. Administration of OLE caused a significant decrease serum AST, ALT enzyme, total bilirubin, GGT, LDH, creatinine, urea, alpha-fetoprotein. Also, amelioration of oxidant ­ antioxidant status with olive leaves extract was observed in addition to a significant decrease in MDA and a significant increase in TAC in liver tissue with a significant increase in glutathione reductase (GR) and SOD in serum compared to paracetamol treated group The chemical pathological changes were in step with histopathological observation suggesting marked hepatoprotective result of olive leaves extract. It could be concluded that olive leaves extract (OLE) treatment may be effective in decreasing hepatic injury and oxidative stress induced by paracetamol overdose in male albino rats


A sobredosagem de paracetamol (PCM) pode causar hepatotoxicidade com estresse oxidativo; o presente estudo foi realizado para estabelecer o possível efeito protetor do extrato de folhas de oliveira (OLE) na toxicidade induzida pelo paracetamol em ratos machos adultos. Vinte e quatro ratos machos adultos foram divididos em quatro grupos iguais: controle, grupo extrato de folhas de oliveira, grupo paracetamol e extrato de folhas de oliveira mais grupo paracetamol. Alguns parâmetros bioquímicos e histopatologia hepática foram avaliados. O tratamento com PCM aumentou significativamente aspartato aminotransferase sérica (AST), alanina aminotransferase (ALT), bilirrubina total, gama-glutamiltransferase (GGT), lactato desidrogenase (LDH), uréia, creatinina e alfa-fetoproteína. Verificou-se que o paracetamol aumenta significativamente o malonaldeído (MDA) e diminui a atividade da glutationa redutase (GR) no tecido e diminui significativamente a capacidade antioxidante total (TAC) e a superóxido dismutase (SOD) no soro. A administração de OLE causou uma diminuição significativa de AST, enzima ALT, bilirrubina total, GGT, LDH, creatinina, uréia, alfa-fetoproteína. Também foi observada melhora do status oxidante - antioxidante com extrato de folhas de oliveira, além de uma diminuição significativa no MDA e um aumento significativo no TAC no tecido hepático, com um aumento significativo na glutationa redutase (GR) e SOD no soro em comparação ao grupo tratado com paracetamol. As alterações patológicas químicas acompanharam a observação histopatológica, sugerindo resultado hepatoprotetor acentuado do extrato de folhas de oliveira. Pode-se concluir que o tratamento com extrato de folhas de oliveira (OLE) pode ser eficaz na diminuição da lesão hepática e do estresse oxidativo induzido pela overdose de paracetamol em ratos albinos machos


Subject(s)
Animals , Rats , Plant Extracts/pharmacology , Olea , Hepatoprotector Drugs , Chemical and Drug Induced Liver Injury/prevention & control , Acetaminophen/toxicity , Rats, Inbred Strains , Plant Extracts/chemistry , Random Allocation , Oxidants , Rats, Wistar , Plant Leaves , Oxidative Stress/drug effects , Hepatocytes/drug effects , Liver/pathology , Antioxidants/pharmacology
2.
Article in English | WPRIM (Western Pacific) | ID: wprim-811066

ABSTRACT

PURPOSE: Asthma control in older asthmatics is often less effective, which may be attributed to small airway dysfunction and poor inhalation technique. We compared the efficacy of 2 inhalers (fluticasone propionate/formoterol treatment using a pressurized metered-dose inhaler [p-MDI group] vs. fluticasone propionate/salmeterol treatment using a dry powder inhaler [DPI group]) in older asthmatics.METHODS: We conducted a 12-week, randomized, open-label, parallel-designed trial in older patients (over 55 years old) with moderate-to-severe asthma, and compared the efficacy and safety for asthma control between the 2 groups. Subgroup analyses on disease duration and air trapping were performed. Clinical parameters, including changes in lung function parameters, inhaler technique and adherence, were compared with monitoring adverse reactions between the 2 groups.RESULTS: A total of 68 patients underwent randomization, and 63 (30 in the p-MDI group and 33 in the DPI group) completed this study. The p-MDI group was non-inferior to the DPI group with regard to the rate of well-controlled asthma (53.3% vs. 45.5%, P < 0.001; a predefined non-inferiority limit of 17%). In subgroup analyses, the proportion of patients who did not reach well-controlled asthma in the p-MDI group was non-inferior to that in the DPI group; the difference was 12.7% among those with a longer disease duration (≥ 15 years) and 17.5% among those with higher air-trapping (RV/TLC ≥ 45%), respectively (a predefined non-inferiority limit of 17%, P < 0.001). No significant differences were observed in lung function parameters, inhalation techniques, adherence and adverse reactions between the 2 groups.CONCLUSION: These results suggest that the p-MDI group may be comparable to the DPI group in the management of older asthmatics in aspects of efficacy and safety.


Subject(s)
Airway Management , Asthma , Dry Powder Inhalers , Fluticasone , Humans , Inhalation , Lung , Medication Adherence , Metered Dose Inhalers , Nebulizers and Vaporizers , Random Allocation
3.
Braz. dent. sci ; 23(1): 1-11, 2020. ilus, tab
Article in English | LILACS (Americas), BBO | ID: biblio-1049834

ABSTRACT

Objective: to evaluate the marginal fit and microleakage of monolithic zirconia crowns cemented by bio-active cements (Ceramir) compared to that cemented with glass ionomer cement, and to evaluate the effect of thermocycling on marginal fit. Material and Methods: Twenty sound human molar teeth were prepared to receive monolithic zirconia crowns. Teeth were divided randomly into two equal groups according to the type of luting cement: Group I (glass ionomer cement) and group II (Ceramir cement). After cementation, the vertical marginal gap was assessed using stereomicroscope before and after thermocycling. Twenty equidistant measurement points were taken for each crown. Leakage assessment was carried out using Fuchsin dye penetration followed by digital photography under a stereomicroscope. Data were analyzed by Mann-Whitney U test to compare between the two luting cements. Wilcoxon signed-rank test was used to evaluate the effect of thermocycling on the marginal fit (P ≤ 0.05). Results: Whether before or after thermocycling, the results showed no significant difference between the marginal gap values of the two tested groups. For both groups, there was a significant increase in marginal gap values after thermocycling. Also, there was no significant difference between leakage scores of the two tested groups. Conclusion: Similarity in the physical properties and chemical composition of the two cements resulted in a nonsignificant effect on the vertical marginal fit and the extent of microleakage of translucent zirconia crowns. Thermocycling had a negative impact on the vertical marginal gap of the two tested luting agents. (AU)


Objetivo: avaliar a adapatação marginal e a microinfiltração de coroas monolíticas de zircônia cimentadas com cimentos bioativos (Ceramir) em comparação com o cimento de ionômero de vidro e avaliar o efeito da termociclagem na adaptação marginal. Material e Métodos: Vinte molares humanos sadios foram preparados para receber coroas monolíticas de zircônia. Os dentes foram divididos aleatoriamente em dois grupos iguais, de acordo com o tipo de cimento: Grupo I (cimento de ionômero de vidro) e grupo II (cimento Ceramir). Após a cimentação, a adaptação marginal vertical foi avaliada com estereomicroscópio antes e após a termociclagem. Vinte pontos de medição equidistantes foram obtidos para cada coroa. A avaliação da infiltração foi realizada utilizando a penetração do corante de fucsina, seguida de fotografia digital sob estereomicroscópio. Os dados foram analisados pelo teste de Mann-Whitney para comparação entre os dois cimentos. O teste de Wilcoxon foi usado para avaliar o efeito da termociclagem na adaptação marginal (P ≤ 0,05). Resultados: Antes ou depois da termociclagem, os resultados não mostraram diferença significativa entre os valores de fenda marginal dos dois grupos testados. Para ambos os grupos, houve um aumento significativo nos valores de fenda marginal após a termociclagem. Além disso, não houve diferença significativa entre os escores de infiltração dos dois grupos testados. Conclusão: A similaridade nas propriedades físicas e na composição química dos dois cimentos resultou em um efeito não significativo na adaptação marginal vertical e na extensão da microinfiltração de coroas translúcidas de zircônia. A termociclagem teve um impacto negativo na fenda marginal vertical dos dois agentes de cimentação testados. (AU)


Subject(s)
Humans , Zirconium , Dental Marginal Adaptation , Crowns , Dental Cements , Dental Leakage , Temperature , In Vitro Techniques , Random Allocation , Glass Ionomer Cements , Molar
4.
Braz. dent. sci ; 23(1): 1-6, 2020. tab, ilus
Article in English | LILACS (Americas), BBO | ID: biblio-1049815

ABSTRACT

Objective: Bisphosphonate-related osteonecrosis of the jaw (BRONJ) is a challenging complication of chronic bisphosphonate (BP) use. The hormone relaxin is able to induce the multistep differentiation process of human osteoclastogenesis, exhibits antifibrotic and anti-inflammatory actions, and promotes vasodilatation, wound healing, and angiogenesis. The present study aimed to evaluate the effects of relaxin in the prevention and management of BRONJ. Material and Methods: Thirty-six male Sprague Dawley rats were randomly divided into four groups. Rats in group 1 (n = 10) received relaxin and BP simultaneously for 12 weeks. Rats in group 2 (n = 10) received injections of BP for 12 weeks, followed by relaxin for another 12 weeks. Rats in group 3 (n = 10) received only BP injections, and those in group 4 (control, n = 6) received only saline. Necrosis and inflammation in the rats' mandibles were evaluated as indicators of BRONJ. Results: Necrosis and inflammation were not detected in group 1 (BP + relaxin). In group 3 (BP only), incidence rates of necrosis and inflammation were 90% and 60%, respectively. Conclusions: Our findings suggest that relaxin may be potently effective in preventing BRONJ and have some benefit in the treatment of existing BRONJ (AU)


Objetivo: A osteonecrose da mandíbula relacionada ao bisfosfonato (BRONJ) é uma desafiadora complicação do uso crônico de bisfosfonato (BP). O hormônio relaxina é capaz de induzir o processo múltiplo de diferenciação da osteoclastogênese humana, exibe ações anti-fibróticas e anti-inflamatórias e promove vasodilatação, cicatrização de feridas e angiogênese. O presente estudo teve como objetivo avaliar os efeitos da relaxina na prevenção e tratamento do BRONJ. Material e Métodos: Trinta e seis ratos Sprague Dawley machos foram divididos aleatoriamente em quatro grupos. Os ratos do grupo 1 (n = 10) receberam relaxina e BP simultaneamente por 12 semanas. Os ratos do grupo 2 (n = 10) receberam injeções de BP por 12 semanas, seguidos de relaxina por mais 12 semanas. Os ratos do grupo 3 (n = 10) receberam apenas injeções de BP e os do grupo 4 (controle, n = 6) receberam apenas solução salina. Necrose e inflamação nas mandíbulas dos ratos foram avaliadas como indicadores de BRONJ. Resultados: Necrose e inflamação não foram detectadas no grupo 1 (BP + relaxina). No grupo 3 (somente BP), as taxas de incidência de necrose e inflamação foram de 90% e 60%, respectivamente. Conclusões: Nossos resultados sugerem que a relaxina pode ser potentemente eficaz na prevenção do BRONJ e ter algum benefício no tratamento do BRONJ existente.(AU)


Subject(s)
Animals , Male , Rats , Relaxin/therapeutic use , Bisphosphonate-Associated Osteonecrosis of the Jaw/therapy , Random Allocation , Rats, Sprague-Dawley , Models, Animal , Bisphosphonate-Associated Osteonecrosis of the Jaw/prevention & control , Bisphosphonate-Associated Osteonecrosis of the Jaw/drug therapy , Jaw/pathology
5.
Braz. dent. sci ; 23(1): 1-7, 2020. tab
Article in English | LILACS (Americas), BBO | ID: biblio-1049603

ABSTRACT

Objective: Evaluate the microtensile bond strength (µTBS) in different dentin thicknesses, under simulated pulpal pressure (SPP), submitted to an adhesive technique using laser irradiation. Material and methods: Forty sound human molars were sectioned and randomly divided into two groups (n=20): Group 1 ­ 1 mm of dentin thickness; Group 2 ­ 2 mm of dentin thickness. Each group was divided into two subgroups (n=10): Subgroup A ­ Absence of SPP; Subgroup P ­ Presence of SPP (15 cm H2 O). The samples were sequentially treated with: 37% phosphoric acid, adhesive system (Adper Single Bond 2), Nd:YAG laser irradiation (60 s, 1064 nm, 10 Hz) using 60 and 100 mJ/pulse energy parameters and photopolymerization (10 s). A composite resin block (Filtek Z350) was built up onto the irradiated area. After 30 days stored in water, the samples were sectioned and submitted to microtensile test (10 kgf load cell, 0.5mm/min). Data were analyzed by twoway ANOVA and Tukey tests. Results: Two-way ANOVA revealed no significant differences for SPP on bond strength. The laser energy parameters indicated that 100 mJ showed greater µTBS means compared to the group irradiated with 60 mJ. The presence of SPP reduced the mean µTBS values. Conclusions: Simulated pulpal pressure did not affect the µTBS using 60 mJ of laser energy parameter. At 100 mJ, the presence of SPP negatively influenced the bond strength, regardless of dentin thickness (AU)


Objetivo: O objetivo deste estudo foi avaliar a resistência adesiva (RA) em diferentes espessuras de dentina, associada à pressão pulpar simulada (PPS), quando submetidos à técnica adesiva por irradiação laser. Material e Métodos: Quarenta molares humanos hígidos foram seccionados e divididos aleatoriamente em dois grupos (n=20): Grupo 1 ­ 1 mm de espessura de dentina; Grupo 2 ­ 2 mm de espessura de dentina. As amostras foram divididas em 2 subgrupos (n=10): Subgrupo A ­ ausência de PPS; Subgrupo P ­ presença de PPS (15 cm de H2 O). As amostras foram tratadas seqüencialmente com: ácido fosfórico 37%, sistema adesivo (Adper Single Bond 2), irradiação com Nd:YAG laser (60 s, 1064 nm, 10 Hz) nos parâmetros de energia de 60 e 100 mJ/pulso e fotopolimerização (10 s). Um bloco de resina composta (Filtek Z350, 3M ESPE) foi confeccionado sobre a área irradiada. Após 30 dias armazenados em água, os espécimes foram seccionados e submetidos ao teste de microtração (carga de 10 kgf, 0.5mm/min). Os dados foram analisados pelos testes ANOVA sob 2 fatores e Tukey (p<0.05). Resultados: ANOVA mostrou que não houve diferenças significativas para PPS na RA. Para os parâmetros de energia do laser, 100 mJ apresentou maiores médias de RA quando comparado ao grupo irradiado à 60 mJ. A presença da PPS reduziu as médias de RA. Conclusão: Pressão pulpar simulada não afetou os valores de resistência adesiva para o grupo irradiado com 60 mJ. Para 100 mJ, a presença da pressão pulpar influenciou negativamente na resistência adesiva, independente das espessuras de dentina.(AU)


Subject(s)
Humans , Dental Cements , Dental Pulp , Dentin , Lasers, Solid-State , Hydrostatic Pressure , Random Allocation , Analysis of Variance , Composite Resins , Dentin/radiation effects , Molar
6.
Article in English | WPRIM (Western Pacific) | ID: wprim-786229

ABSTRACT

Cardiovascular disease (CVD) is considered a primary driver of global mortality and is estimated to be responsible for approximately 17.9 million deaths annually. Consequently, a substantial body of research related to CVD has developed, with an emphasis on identifying strategies for the prevention and effective treatment of CVD. In this review, we critically examine the existing CVD literature, and specifically highlight the contribution of Mendelian randomization analyses in CVD research. Throughout this review, we assess the extent to which research findings agree across a range of studies of differing design within a triangulation framework. If differing study designs are subject to non-overlapping sources of bias, consistent findings limit the extent to which results are merely an artefact of study design. Consequently, broad agreement across differing studies can be viewed as providing more robust causal evidence in contrast to limiting the scope of the review to a single specific study design. Utilising the triangulation approach, we highlight emerging patterns in research findings, and explore the potential of identified risk factors as targets for precision medicine and novel interventions.


Subject(s)
Artifacts , Bias , Cardiovascular Diseases , Mendelian Randomization Analysis , Mortality , Precision Medicine , Random Allocation , Risk Factors
7.
Article in English | WPRIM (Western Pacific) | ID: wprim-811205

ABSTRACT

BACKGROUND: This study evaluated the efficacy of three intraoral topical anesthetics in reducing the injection needle prick pain from local anesthetic among children aged 7–11 years old.METHODS: It is a prospective, Interventional, parallel design, single-blind, randomized clinical trial in which subjects (n=90) aged 7–11 years were included in the study based on an inclusion criteria. Subjects were divided into three groups based on computer-generated randomization with an allocation ratio of 1:1:1. Groups A, B, and C received benzocaine 20% jelly (Mucopain gel, ICPA health products Ltd, Ankleshwar, India), cetacaine anesthetic liquid (Cetylite Industries, Inc, Pennsauken, NJ), and EMLA cream (2% AstraZeneca UK Ltd, Luton, UK), respectively, according to manufacturer's instructions, for 1 minute prior to local anesthetic injection. After application of topical anesthetic agent, for all the groups, baseline pre-operative (prior to topical anesthetic administration) and post-operative scores (after local anesthetic administration) of pulse rate was recorded using Pulse oximeter (Gibson, Fingertip Pulse Oximeter, MD300C29, Beijing Choice Electronic). Peri-operative (i.e., during the administration of local anesthesia) scores were recorded using Face, Legs, Activity, Cry, Consolability (FLACC) Scale, Modified Children hospital of Eastern Ontario Pain Scale (CPS) behavior rating scale, and Faces Pain Scale (FPS-R) – Revised (For self-reported pain). Direct self-reported and physiological measures were ascertained using FPS-R – Revised and Pulse oximeter, respectively, whereas CPS and FLACC scales assessed behavioral measures. To test the mean difference between the three groups, a one way ANOVA with post hoc tests was used. For statistical significance, a two-tailed probability value of P < 0.05 was considered as significant.RESULTS: The Cetacaine group had significantly lower pain scores for self-report (P < 0.001), behavioral, and physiological measures (P < 0.001) than the other two groups. However, there was no significant difference between the Benzocaine group and EMLA group during palatal injection prick.CONCLUSION: Cetacaine can be considered as an effective topical anesthetic agent compared to benzocaine 20% jelly (Mucopain gel) and EMLA cream.


Subject(s)
Anesthetics , Behavior Rating Scale , Beijing , Benzocaine , Child , Clinical Study , Heart Rate , Humans , Leg , Needles , Ontario , Palate , Prospective Studies , Random Allocation , Weights and Measures
8.
Arq. ciências saúde UNIPAR ; 23(3)set-dez. 2019.
Article in Portuguese | LILACS (Americas) | ID: biblio-1046203

ABSTRACT

A mobilidade diafragmática é essencial para a ventilação pulmonar. Pela ultrassonografia sua mensuração é direta, porém o processamento das medidas encontra-se em divergência na literatura. Indica-se pelo valor médio das três incursões respiratórias máximas ou o maior valor dentre elas restringindo à variações de 10%. Dessa forma, não existe um consenso em relação ao processamento da medida de mobilidade diafragmática máxima. Objetivo: Comparar dois diferentes processamentos das medidas pela ultrassonografia para o maior valor de mobilidade diafragmática. Materiais e métodos: Estudo observacional transversal. Avaliou-se a mobilidade diafragmática pela ultrassonografia, com um transdutor convexo (3 MHz) posicionado anteriormente na região subcostal e leve inclinação cranial, em decúbito dorsal. Visualizou-se o hemidiafragma direito pelo ponto médio entre a linha médio clavicular e axilar anterior. Para visualizar a janela do diafragma e mensurar sua mobilidade foi utilizado o modo B, seguido do modo M. Os participantes realizaram inspirações máximas e os maiores valores com diferença máxima de 10% entre eles mensurados e registrados. Para análise, o maior valor e o valor médio obtido das três medidas foram considerados. Para normalidade dos dados foi realizado o teste de Shapiro Wilk. Para diferenças entre os registros, o teste de t student. Resultados: 30 indivíduos (30,33 ± 9,7 anos), 16 mulheres e 14 homens. A medida da mobilidade diafragmática pelo maior valor em comparação ao valor médio apresentou diferença estatisticamente significante (8,11 ± 1,43 cm versus 7,79 ± 1,43 cm; p<0,001). Conclusão: O valor máximo da mobilidade diafragmática foi obtido por meio da análise do maior valor. Ao escolher a média, a mobilidade diafragmática pode ser subestimada. 


Diaphragmatic mobility is essential to pulmonary ventilation. It can be directly measured by using ultrasonography, but the processing of the measurements can be found described differently in the literature. It can be measured as the average of at least three different cycles or from the greatest value among them resticting it to a 10% variation. Thus, there is no consensus about the processing of the maximum measurement of diaphragmatic mobility. Objective: Comparisson of two differents ultrasound measurement processings aiming at the diaphragmatic mobility maximum value. Methodology: Cross-sectional observational study. The diaphragmatic mobility was assessed by ultrasonography with convex transducer (3MHz) placed on the subcostal region between the midclavicular and anterior axillary. In order to explore the right diaphragmatic window and mobility, the B mode was used, followed by the M mode. The participants made maximum inspiration, and the highest value with a maximum difference of 10% was recorded. For statistical analysis, the mean and the highest value of three measurements were considered. The data distribution was analyzed with a Shapiro Wilk test and differences among records by the t student test. Results: 30 participants (30.33 ± 9.7 years) - 16 women and 14 men. The measurement of the diaphragmatic mobility obtained by the highest value compared against the mean value presented a statistically significant difference (8.11 ± 1.43 cm vs 7.79 ± 1.43 cm; p<0.001). Conclusions: The maximum value of diaphragmatic mobility was obtained by the analysis of the highest value. By choosing to use the mean value, diaphragmatic mobility may be underestimated.


Subject(s)
Humans , Male , Female , Adult , Young Adult , Diaphragm/physiology , Reference Standards , Respiration , Diaphragm/diagnostic imaging , Random Allocation , Cross-Sectional Studies , Ultrasonography , Healthy Volunteers , Organ Motion
9.
Acta cir. bras ; 34(11): e201901105, Nov. 2019. graf
Article in English | LILACS (Americas) | ID: biblio-1054679

ABSTRACT

Abstract Purpose: To evaluate the effects of Dexmedetomidine (Dex) on spinal pathology and inflammatory factor in a rat model of Diabetic neuropathic pain (DNP). Methods: The rats were divided into 3 groups (eight in each group): normal group (N group), diabetic neuropathic pain model group (DNP group), and DNP model with dexmedetomidine (Dex group). The rat model of diabetes was established with intraperitoneal streptozotocin (STZ) injections. Nerve cell ultrastructure was evaluated with transmission electron microscopy (TEM). The mechanical withdrawal threshold (MWT) and motor nerve conduction velocity (MNCV) tests documented that DNP rat model was characterized by a decreased pain threshold and nerve conduction velocity. Results: Dex restored the phenotype of neurocytes, reduced the extent of demyelination and improved MWT and MNCV of DNP-treated rats (P=0.01, P=0.038, respectively). The expression of three pain-and inflammation-associated factors (P2X4, NLRP3, and IL-IP) was significantly upregulated at the protein level in DNP rats, and this change was reversed by Dex administration (P=0.0022, P=0.0092, P=0.0028, respectively). Conclusion: The P2X4/NLRP3 signaling pathway is implicated in the development and presence of DNP in vivo, and Dex protects from this disorder.


Subject(s)
Animals , Male , Spine/drug effects , Dexmedetomidine/pharmacology , Diabetic Neuropathies/drug therapy , Receptors, Purinergic P2X4/analysis , Adrenergic alpha-2 Receptor Agonists/pharmacology , NLR Family, Pyrin Domain-Containing 3 Protein/analysis , Sural Nerve/drug effects , Time Factors , Random Allocation , Blotting, Western , Pain Threshold , Microscopy, Electron, Transmission , Diabetes Mellitus, Experimental/pathology , Diabetes Mellitus, Experimental/drug therapy , Diabetic Neuropathies/pathology , Disease Models, Animal , Interleukin-1beta/analysis , Interleukin-1beta/drug effects , NLR Family, Pyrin Domain-Containing 3 Protein/drug effects , Neural Conduction/drug effects
10.
Acta cir. bras ; 34(11): e201901103, Nov. 2019. tab, graf
Article in English | LILACS (Americas) | ID: biblio-1054680

ABSTRACT

Abstract Purpose: To evaluate liver regeneration after selective ligation of portal vein and hepatic artery by 3D Computed Tomography in an experimental model. Methods: Sixteen Wistar rats were randomized into four equal groups: Group I- control (sham), Group II- isolated selective ligation of the hepatic artery, Group III- isolated selective ligation of the portal vein and Group IV- combined ligation of portal vein and hepatic artery. Before procedure and five days after a 3D CT Scan was performed to analyze the hypertrophy, weight and function of the remnant liver. Results: The largest regeneration rate and increase of weight in the hypertrophied lobe was detected in group IV, the first with an average of 3.99 (p=0.006) and the last varying from 6.10g to 9.64g (p=0.01). However, total liver weight and the R1 ratio (Hypertrophied Lobe Weight/Total Liver Weight) was higher in group III (P<0.001) when compared with groups I, II and IV and showed no difference between them. The immunohistochemical examination with PCNA also found higher percentages with statistical significance differences in rats of groups III and IV. It was possible to confirm a strong correlation between hypertrophied lobe weight and its imaging volumetric study. Liver function tests only showed a significant difference in serum gamma-glutamyltransferase and phosphorous. Conclusion: There is a largest liver regeneration after combined ligation of portal vein and hepatic artery and this evidence may improve the knowledge of surgical treatment of liver injuries, with a translational impact in anima nobile.


Subject(s)
Animals , Male , Portal Vein/surgery , Hepatic Artery/surgery , Liver/diagnostic imaging , Liver Regeneration/physiology , Organ Size/physiology , Immunohistochemistry , Random Allocation , Tomography, X-Ray Computed/methods , Reproducibility of Results , Treatment Outcome , Rats, Wistar , Imaging, Three-Dimensional/methods , Hepatomegaly/physiopathology , Hepatomegaly/diagnostic imaging , Ligation , Liver/blood supply , Liver/pathology
11.
Acta cir. bras ; 34(11): e201901102, Nov. 2019. tab, graf
Article in English | LILACS (Americas) | ID: biblio-1054682

ABSTRACT

Abstract Purpose: To investigate the effect of Picroside II on testicular ischemia and reperfusion (l/R) injury and the underlying mechanism. Methods: Sprague-Dawley rats were randomly divided into 4 groups: sham operated group (Sham), Sham with Picroside II treatment group (Sham+ Pic II), l/R group (l/R) and l/R with Picroside II treatment group (I/R+ Pic II). l/R model was established by rotating the left testis 720° in a clock-wise direction for 4 hours. The histopathologic and spermatogenetic evaluation was performed. The apoptosis changes and the levels of HO-1 (heme oxygenase-1), MPO (myeloperoxidase), NOX (NADPH oxidase), SOD (superoxide dismutase), XO (xanthine oxidase) and NOS (nitric oxide synthase) were measured. Results: The seminiferous tubules were damaged in l/R rats, but Picroside II alleviated the changes induced by l/R. The increased level of apoptosis was decreased by Picroside II (P=0.01, 9.05±0.35 vs. 4.85±0.25). The activities of HO-1, MPO, NOX, XO and MDA content were increased and the SOD activity was decreased in l/R (P<0.05) and could be reversed by Picroside II (P=0.03, 405.5±7.5 vs. 304±17U/mgprot; P=0.02, 0.99±0.05 vs. 0.52±0.04 mgprot; P=0.01, 260+7 vs. 189±2 mgprot; P=0.04, 10.95+0.55 vs. 8.75+0.35 U/mgprot; P=0.045, 6.8+0.7 vs. 3.75+0.35 mgprot; P=0.04, 44.5+3.5 vs. 57.5+3.5 mgprot). Western blot showed that the expression of iNOS, nNOS and eNOS were increased in l/R (P<0.05); however, they were decreased after Picroside II treatment (P<0.05). Conclusion: Picroside II attenuated testicular I/R injury in rats mainly through suppressing apoptosis and oxidative stress through reduction of nitric oxide synthesis.


Subject(s)
Animals , Male , Testis/blood supply , Reperfusion Injury/prevention & control , Cinnamates/pharmacology , Apoptosis/drug effects , Oxidative Stress/drug effects , Iridoid Glucosides/pharmacology , Nitric Oxide/biosynthesis , Reperfusion Injury/metabolism , Reperfusion Injury/pathology , Random Allocation , Blotting, Western , Rats, Sprague-Dawley , Peroxidase/analysis , In Situ Nick-End Labeling , Heme Oxygenase-1/analysis , Malondialdehyde/analysis , NADP/analysis
12.
Braz. j. biol ; 79(3): 423-431, July-Sept. 2019. tab, graf
Article in English | LILACS (Americas) | ID: biblio-1001462

ABSTRACT

Abstract The usage of aquatic plants represents an alternative in the treatment of residues originating from swine. In these systems, one of the N removal methods is the ammonium (NH4 +) uptake and volatilization of ammonia (NH3). In this way, the objective of this work was to evaluate the volatilization rates of NH3 in waste treatment systems swine fluids (SSF) with aquatic macrophytes, as well as the concentration of NH 4+ present in the swine fluids. The experiment was carried out at Campus II/UNOESTE. The treatment systems were composed of 16 boxes of PVC and characterized as: T1 = Control sample 50% of SSF/50% of water; T2 = 50% SSF/50% water + Eichhornia crassipes (Mart.) Solms; T3 = 50% SSF/50% water + Pistia stratiotes L.; T4 = 50% SSF/50% water + Salvinia auriculata Aubl. The design was randomized blocks, with 4 treatments and 4 replicates. The hydrogen potential (pH) and the NH4 + content of the effluent were analyzed weekly, and the volatilization of NH 3 by means of collectors installed in each treatment unit. The presence of aquatic macrophytes promoted the reduction of NH4+ concentration and of the pH values ​​of swine fluids, and this resulted in the reduction of NH3 volatilization rates to the environment, with emphasis on the system with Eichhornia crassipes (Mart.) Solms, which presented the lowest rate of volatilization.


Resumo A utilização de plantas aquáticas representa uma alternativa no tratamento de resíduos oriundos da suinocultura. Nestes sistemas, uma das formas de remoção de nitrogênio (N) é a absorção de amônio (NH4+) pelas plantas, entretanto, também ocorre a volatilização de amônia (NH3). Dessa forma, o objetivo do trabalho foi avaliar as taxas de volatilização de NH3 em sistemas de tratamentos de dejetos líquidos de suínos (DLS) com macrófitas aquáticas, bem como a concentração de NH4+ presente nos dejetos. O experimento foi realizado em área de ambiente aberto no Campus II/UNOESTE. Os sistemas de tratamento foram constituídos de 16 caixas de PVC e caracterizados como: T1 = Testemunha 50% de DLS/50% de água; T2 = 50% de DLS/50% de água + Eichhornia crassipes (Mart.) Solms; T3 = 50% de DLS/50% de água + Pistia stratiotes L.; T4 = 50% de DLS/50% de água + Salvinia auriculata Aubl. O delineamento adotado foi em blocos casualizados, com 4 tratamentos e 4 repetições. Foram analisados o potencial hidrogeniônico (pH) e o teor de NH4 + do efluente semanalmente, e a volatilização de NH3 por meio de coletores instalados em cada unidade de tratamento. A presença das macrófitas aquáticas proporcionou a redução da concentração de NH4+ e dos valores de pH dos dejetos líquidos de suínos, e isto resultou na redução das taxas de volatilização de NH 3 ao meio ambiente, com destaque ao sistema com Eichhornia crassipes (Mart.) Solms, que apresentou a menor taxa de volatilização.


Subject(s)
Animals , Waste Disposal, Fluid , Araceae/metabolism , Eichhornia/metabolism , Ammonia , Manure/analysis , Swine , Volatilization , Biodegradation, Environmental , Random Allocation , Water Consumption (Environmental Health) , Industrial Waste/analysis , Animal Husbandry
13.
Braz. j. biol ; 79(3): 505-509, July-Sept. 2019. tab, graf
Article in English | LILACS (Americas) | ID: biblio-1001446

ABSTRACT

Abstract Salt water recirculation systems using automatic feeders are a new frontier for marine aquaculture. It is possible to decrease the vulnerability of the traditional fish farming in open systems and reduce wasteful feeding and discharge of effluents, as well as increase the economic returns. An experiment with common snook fingerings (4.31 ± 1.42 g and 8.4 ± 1.0 cm) was performed. Three feeding treatments were evaluated: six, twelve and eighteen feeding times per day. Each treatment had three replications. Among the three tested frequencies (fed 6, 12 and 18 times a day), the feeding frequency of 12 times per day (every two hours) showed the best values of the parameters evaluated for production performance. The final weight values, condition factor, specific growth rate and daily weight gain of the treatment 12 feeding times per day were all significantly higher than the lower and higher frequencies. Our results demonstrate that high feeding frequency is not synonymous of higher performance, but is extremely necessary to find out the best range for the target species. Besides, this relationship between feeding frequency and growth performance might change over weight range. Therefore, future studies should address higher weight ranges for comparison to our results.


Resumo Sistemas de recirculação de água salgada utilizando alimentadores automáticos são uma nova fronteira para a aquicultura marinha. Possibilitam diminuir a vulnerabilidade dos cultivos em sistemas abertos e conferir redução de desperdício de ração e lançamento de efluentes, assim como elevar o retorno econômico. Foi realizado um experimento com juvenis de Robalo-flecha (4,31 ± 1,42 g e 8,4 ± 1,0 cm). Três frequências alimentares foram avaliadas: alimentação seis vezes ao dia; doze vezes ao dia, e; dezoito vezes ao dia. Cada tratamento teve três repetições. Dentre as três frequências avaliadas a frequência alimentar de 12 vezes ao dia foi a que apresentou os melhores valores para os parâmetros zootécnicos avaliados. Os valores obtidos de peso final, fator de condição, taxa de crescimento específico e ganho de peso diário foram todos maiores para a frequência de 12 vezes ao dia do que os obtidos para as frequências maior e menor. Nossos resultados demonstram que elevadas frequências alimentares não são sinônimo de melhor desempenho produtivo, mas é extremamente importante conhecer a frequência ideal para a espécie a ser cultivada. Além disto, a relação entre frequência alimentar e crescimento pode mudar de acordo com a faixa de peso dos indivíduos. Desta forma, é extremamente importante que trabalhos futuros avaliem as frequências aqui estudadas para comparar os resultados em indivíduos maiores.


Subject(s)
Animals , Perciformes/growth & development , Aquaculture/methods , Diet/veterinary , Random Allocation , Animal Feed/analysis
14.
Rev. Paul. Pediatr. (Ed. Port., Online) ; 37(2): 217-224, Apr.-June 2019. tab
Article in English | LILACS (Americas) | ID: biblio-1013286

ABSTRACT

ABSTRACT Objective: To analyze the risk behavior for bulimia among female adolescents from public and private high schools. Methods: A cross-sectional study with a random sample of 850 female students aged 15-18 years was carried out in a city in northeastern Brazil, using the Bulimic Investigatory Test of Edinburgh (BITE) to assess the risk behavior for bulimia. Data were analyzed using the Statistical Package for the Social Sciences (SPSS) software and the Pearson's chi-square , Fisher's exact and robust Poisson regression tests, adopting the significance level of 5%. Results: Less than half of the sample (42.0%) showed standards of dietary risk and weight control practices; in 1.4% of the sample, bulimia signs were already installed. Fear of gaining weight was reported by 62.8% of the subjects. Risk practices were lower among students from public schools; (Odds Ratio - OR - 0.82; confidence interval of 95% - 95%CI - 0.69-0.97). Among restrictive practices, fasting for a whole day was the most applied (29.9% of the students). Among individuals who were at risk situation, almost half believed to have normal eating habits (prevalence ratio - PR - 0.42; 95%CI 0.36-0.49). Individuals who consider their eating habits normal, who are afraid of gaining weight, those who seek emotional comfort in food and follow strict diets had higher risk for bulimia (p<0.05). Conclusions: The number of female adolescent students with risk behavior practices for bulimia is high, and the frequency of those unaware of this situation is also very high. Risk situations emerge as a collective health problem, and individuals from private schools were more likely to be in this situation.


RESUMO Objetivo: Analisar comportamentos de risco para bulimia em adolescentes do sexo feminino de escolas públicas e particulares. Métodos: Estudo transversal com amostra aleatória de 850 estudantes do sexo feminino, com idades entre 15 e 18 anos, realizado em cidade do Nordeste do Brasil, utilizando o Bulimic Investigatory Test of Edinburgh (BITE) para avaliar comportamentos de risco para transtornos alimentares. Os dados foram analisados utilizando o teste do qui-quadrado de Pearson, o teste exato de Fisher e a regressão de Poisson, com o software Statistical Package for the Social Sciences (SPSS), adotando o nível de significância de p<0,05. Resultados: Da amostra estudada, 42,0% apresentou padrões de risco e práticas de dieta e controle de peso e 1,4% já apresentava sinais de bulimia instalados. O medo de ganhar peso foi relatado por 62,8% das adolescentes. As práticas de risco foram menos frequentes em estudantes de escolas públicas (Odds Ratio - OR - 0,82; intervalo de confiança de 95% - IC95% - 0,69-0,97). Entre as práticas restritivas, jejum por um dia inteiro foi o mais aplicado pelas participantes (29,9%). Entre os indivíduos com situação de risco, quase metade acreditava ter hábitos alimentares normais (razão de prevalência - RP - 0,42; IC95% 0,36-0,49). Estudantes que consideram seus hábitos alimentares normais, que têm medo de ganhar peso, que procuram conforto emocional em alimentos e seguem dietas rigorosas tiveram maior risco para bulimia (p<0,05). Conclusões: O número de estudantes com práticas de comportamento de risco para bulimia é alto, e o número daquelas que desconhecem essa situação também é muito alto. As situações de risco emergem como problemas de saúde coletiva, e indivíduos de escolas particulares são mais propensos a apresentar transtornos alimentares.


Subject(s)
Humans , Female , Adolescent , Adolescent Behavior/psychology , Life Style , Brazil/epidemiology , Bulimia/psychology , Bulimia/epidemiology , Random Allocation , Prevalence , Cross-Sectional Studies , Risk Assessment , Adolescent Health/statistics & numerical data , Diet, Reducing/adverse effects , Diet, Reducing/psychology , Feeding Behavior/physiology , Feeding Behavior/psychology
15.
Article in Chinese | WPRIM (Western Pacific) | ID: wprim-771509

ABSTRACT

This research was aimed to evaluate the protective effect and potential mechanism of Yiqi Tongluo Particles(YQTLs).Firstly,an animal model of multiple cerebral infarction(MCI) with Qi deficiency and blood stasis was established.Rats were randomly divided into six groups:SHAM group,Vehicle group,Buyang Huanwu decoction original group(BYHWO),EGb761 group,high and low dose of YQTLs group.Rats underwent sleep deprivation after one week of MCI and the tongues and pulses of rats after six weeks of sleep deprivation were detected,followed by collecting blood to analysis the blood coagulation.Differential expression of angiogenesis associated proteins was examined using proteomic research and verified by immunohistochemical.RESULTS: showed that neurological function score was obviously declined,G and B value of tongue surface was increased significantly and the pulse distension,the activated partial thromboplatin time(APTT) as well as prothrombin time(PT) were recovered following YQTLs 7.56 g·kg-1 treatment.Furthermore,G value of tongue surface,APTT and PT were also improved by YQTLs 3.78 g·kg-1.The results of proteomic technology showed that proteins associated with angiogenesis were reversed compared with Vehicle group.Moreover,the expression of VEGFR2 from immunohistochemical was promoted after YQTLs treatment.The MCI with Qi deficiency and blood stasis was alleviated obviously following YQTLs treatment and the possible mechanism was that YQTLs may enhance angiogenesis during cerebral ischemia.


Subject(s)
Angiogenesis Inducing Agents , Pharmacology , Animals , Cerebral Infarction , Drug Therapy , Drugs, Chinese Herbal , Pharmacology , Proteomics , Qi , Random Allocation , Rats
16.
Acta Physiologica Sinica ; (6): 839-845, 2019.
Article in Chinese | WPRIM (Western Pacific) | ID: wprim-781391

ABSTRACT

The purpose of this study was to explore the effects of calcitonin gene-related peptide (CGRP) on the long-term depression (LTD) of hippocampus in mice. Sixty C57BL/6J mice (30 days old) were randomly divided into control group, three CGRP (50, 100, and 200 nmol/L) groups, CGRP + CGRP group and CGRP + APV group (10 mice for each group). The effects of exogenous application of different concentrations of CGRP on synaptic plasticity and LTD in hippocampus of mice were detected by in vitro recording of local field potential. The results showed that higher doses (100 and 200 nmol/L) of CGRP significantly enhanced the induction of LTD in the hippocampus. Moreover, CGRP increased the magnitude of N-methyl-D-aspartate (NMDA) receptor-mediated excitatory postsynaptic currents. The above-mentioned effects of CGRP were blocked by either CGRP selective antagonist CGRP or NMDA receptor antagonist APV. These results suggest that CGRP can dose-dependently enhance the induction of LTD in hippocampus of mice, and the underlying mechanism involves the mediation of NMDA receptor function.


Subject(s)
Animals , Calcitonin Gene-Related Peptide , Pharmacology , Hippocampus , Long-Term Synaptic Depression , Mice , Mice, Inbred C57BL , Random Allocation
17.
Article in Chinese | WPRIM (Western Pacific) | ID: wprim-776253

ABSTRACT

OBJECTIVE@#To explore the action mechanism of acupoint selection along meridians to improve adenosine receptor in myocardial ischemia (MI) rats by comparing the effects of acupoint selection along meridians, acupoint selection at other meridians and non-acupoint on expression of adenosine receptor.@*METHODS@#A total of 120 SD rats were randomly divided into a blank group, a sham operation group, a model group, an acupoint-selection-along-meridians (ASAM) group, an acupoint-selection-at-other-meridians (ASAOM) group and a non-acupoint group, 20 rats in each group. The model of MI was not made in the blank group; the left anterior descending coronary artery was not ligated after thoracotomy in the sham operation group; the model of MI was made but acupuncture was not given in the model group. After the model of MI was made, electroacupuncture (EA) was applied at "Neiguan" (PC 6) in the ASAM group, at "Hegu" (LI 4) in the ASAOM group, and at the area between the third and fourth metatarsal bone in the non-acupoint group. EA was given 20 min per treatment, once a day for 5 days. After treatment, the TTC staining was used to detect myocardial infarction, the Tunel method was used to detect cardiomyocyte apoptosis, and the immunohistochemistry was used to detect the expression of adenosine receptors A1, A2a and A2b.@*RESULTS@#Compared with the blank group and the sham operation group, the percentage of myocardial infarction and apoptotic rate of myocardial cells in the model group were increased significantly (<0.01). After EA treatment, compared with the model group, the percentage of myocardial infarction and apoptotic rate of myocardial cells in the ASAM group were decreased significantly (<0.01), and the expression levels of adenosine receptors A1, A2a and A2b were increased significantly (<0.01). The percentage of myocardial infarction and apoptotic rate of myocardial cells in the ASAM group were significantly lower than those in the ASAOM group and the non-acupoint group (<0.01), and the expression levels of adenosine receptors A1, A2a and A2b in the ASAM group were significantly higher than those in the ASAOM group and non-acupoint group (<0.01).@*CONCLUSION@#Compared with acupoint selection at other meridians or non-acupoints, acupoint selection along meridians can effectively regulate the expression of adenosine receptors A1, A2a and A2b, improve the condition of myocardial infarction, inhibit myocardial cell apoptosis, and consequently protect ischemic myocardium.


Subject(s)
Acupuncture Points , Animals , Electroacupuncture , Humans , Meridians , Myocardial Ischemia , Therapeutics , Random Allocation , Rats , Rats, Sprague-Dawley , Receptors, Purinergic P1
18.
Article in Chinese | WPRIM (Western Pacific) | ID: wprim-776252

ABSTRACT

OBJECTIVE@#To explore the protective mechanism of electroacupuncture preconditioning on myocardial ischemia-reperfusion injury in rats.@*METHODS@#A total of 88 SPF-grade Wistar male rats were randomized into a normal group, a sham-operation group, a model group and an electroacupuncture (EA) group, 22 rats in each one. The rats in the normal group received normal diet and no intervention was given for 7 d. The rats in the sham-operation and the model group were bound for 20 min, once a day for 7 d. In the EA group, EA, continuous wave, 2 Hz and 1 mA, was applied at "Neiguan" (PC 6), "Zusanli" (ST 36) and "Guanyuan" (CV 4) for 20 min, once a day for 7 d. In the 8th day, the blood was collected by abdominal aortic method 60 min after intraperitoneal injection of 10% urethane anesthesia by 10 mL/kg in the normal group, and then the tissue of heart was collected. In the model group and the EA group, ligating the left anterior descending coronary artery (LAD) was adopted for 20 min and reperfusion was applied for 40 min, then the samples were collected. The rats in the sham-operation group recevied open-chest operation and no other intervention, the samples were collected in 60 min. Nitro Blue Tetrazolium Chloride monohydrate (NBT) stain was used to detected myocardial infarct size and weight, ELISA was used to measured myocardial injury markers and inflammatory factors (LDH、CK、cTnI), and the fluorescent quantitative PCR method was used to determine the expressions of FXR and SHP gene.@*RESULTS@#①There was no significant difference between the normal group and the sham-operation group in myocardial infarct size and weight (>0.05) .Compared with the normal group, myocardial infarct size and weight were higher in the model group (0.05). Compared with the normal group, the serum contents of LDH、CK、cTnI were higher in the model group (<0.05). Compared with the model group, the serum contents of LDH、CK、cTnI were lower in the EA group (<0.05). ③The expressions of FXR and SHP gene in the model group were higher than those in the normal group (<0.05). The expressions of FXR and SHP gene in the EA group were lower than those in the model group (<0.05).@*CONCLUSION@#Electroacupuncture preconditioning could significantly improve cardiac function of rats with myocardial ischemia-reperfusion, reduce infarct size and inflammatory factors, and down-regulate the expressions of FXR and SHP gene. The protective effect may generate based on regulation of FXR/SHP apoptosis signal pathway.


Subject(s)
Animals , Electroacupuncture , Humans , Male , Myocardial Ischemia , Myocardial Reperfusion Injury , Therapeutics , Random Allocation , Rats , Rats, Sprague-Dawley , Rats, Wistar
19.
Article in Chinese | WPRIM (Western Pacific) | ID: wprim-776233

ABSTRACT

OBJECTIVE@#To explore the protective effect and apoptosis-related mechanism of electroacupuncture (EA) preconditioning in the rats with cerebral ischemia-reperfusion injury.@*METHODS@#Sixty male SD rats, 3 months old, at SPF grade were randomized into a sham-operation group, an ischemia-reperfusion group and an EA preconditioning group, 20 rats in each one. In the ischemia-reperfusion group and EA preconditioning group, the modified MCAO suture-occlusion method was adopted to exert ischemia for 2 h and reperfusion for 3 h, and thus, the models of focal cerebral ischemia-reperfusion injury were prepared on the right side. In the sham-operation group, the right common carotid artery was separated and no more management was given. In the EA preconditioning group, EA at "Baihui" (GV 20), "Shenshu" (BL 23) and "Sanyinjiao" (SP 6) was provided before modeling, with disperse-dense wave, at 2 Hz/100 Hz, 1 mA in intensity. The stimulation for 15 min was taken as one unit (meaning electric stimulation for 10 min and needle retaining for 5 min without electric stimulation). Such preconditioning was repeated continuously for 4 times, totally for 1 h. The neuroethologic condition was assessed in 3 h of reperfusion in each group. TTC staining method was used to determine the percentage of cerebral infarction zone, TUNEL method was to determine the apoptosis index (AI) in hippocampal neuron and the immunohistochemical method (IHC) was to determine the protein expression of p53 and caspase-3.@*RESULTS@#Compared with the sham-operation group, the neuroethologic score, the percentage of cerebral infarction zone and neuronal AI were all increased obviously in the ischemia-reperfusion group (all <0.01). Compared with the ischemia-reperfusion group, the neuroethologic score, the percentage of cerebral infarction zone and neuronal AI were all reduced obviously in the EA preconditioning group (all <0.01). p53's nuclei and caspase-3's cytoplasms were stained. The positive cells of both of them were brown-yellow in color. In the sham-operation group, the structure of the right hippocampal CA3 neurons of rats was clear, with few positive cells. In the ischemia-perfusion group, the positive expressions of p53 and caspase-3 in the right hippocampal CA3 were increased obviously (<0.01). Compared with the ischemia-reperfusion group, the positive expressions of caspase-3 and p53 in the right hippocampal CA3 were significantly reduced in the EA preconditioning group (<0.01).@*CONCLUSION@#Electroacupuncture preconditioning relieves ischemic injury in brain tissue of rats probably through inhibiting the expressions of p53 and caspase-3 to resisting neuronal apoptosis.


Subject(s)
Acupuncture Points , Animals , Brain Ischemia , Caspase 3 , Physiology , Electroacupuncture , Hippocampus , Humans , Male , Neurons , Random Allocation , Rats , Rats, Sprague-Dawley , Reperfusion Injury , Tumor Suppressor Protein p53 , Physiology
20.
Article in Chinese | WPRIM (Western Pacific) | ID: wprim-776232

ABSTRACT

OBJECTIVE@#To observe the repairing effects of estrogen and wheat-grain moxibustion on thin-type endometrium in rats and to explore its possible mechanism.@*METHODS@#Forty healthy SPF-grade adult female SD rats were randomly divided into a normal group, a model group, an estrogen group and a moxibustion group according to random number table method, 10 rats in each group. The model of thin-type endometrium was established during estrous period in all the groups except for the normal group. No intervention was given in the normal group. The intragastric administration of 2 mL of 0.9% sodium chloride solution was applied the next day after modeling in the model group. The intragastric administration of 2 mL of estradiol was given the next day after modeling in the estrogen group. The wheat-grain moxibustion was given at "Guanyuan" (CV 4) and "Shenshu" (BL 23) the next day after modeling in the moxibustion group, 7 moxa cones for each acupoint. The treatment in 3 groups was given once a day. After three estrous cycles, the samples were collected during estrous period; the thickness and morphology of endometrium were observed by HE staining; the expressions of vimentin, keratin and vascular endothelial growth factor (VEGF) in endometrium tissue were detected by immunohistochemistry; the expressions of HOXA10 and LIF in endometrium tissue were detected by Western blot.@*RESULTS@#The endometrial thickness in the model group was significantly thinner than that in the normal group (0.05). The expressions of keratin, vimentin and VEGF in endometrium in the model group were significantly lower than those in the normal group (0.05). The expressions of HOXA10 and LIF in endometrium in the model group were significantly lower than those in the normal group (<0.01); compared with the model group, the expressions of HOXA10 and LIF in endometrium in the estrogen group and moxibustion group were significantly increased (<0.05).@*CONCLUSION@#The wheat-grain moxibustion could up-regulate the expressions of keratin, vimentin and VEGF in endometrium to improve the endometrial thickness; in addition, it could increase the levels of factors related to endometrial receptivity including HOXA10, LIF, which improves endometrial receptivity and play a repair role.


Subject(s)
Animals , Endometrium , Physiology , Female , Moxibustion , Random Allocation , Rats , Rats, Sprague-Dawley , Triticum , Vascular Endothelial Growth Factor A
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