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1.
J. appl. oral sci ; 29: e20210138, 2021. tab, graf
Article in English | LILACS | ID: biblio-1340112

ABSTRACT

Abstract Mesenchymal and epithelial stem cells were identified in dental tissues; however, knowledge about the odontogenic stem cells is limited, and there are some questions regarding their temporo-spatial dynamics in tooth development. Objective Our study aimed to analyze the expression of the stem cell markers CD146 and p75NTR during the different stages of odontogenesis. Methodology The groups consisted of 13.5, 15.5, 17.5 days old embryos, and 14 days postnatal BALB/c mice. The expression of CD146 and p75NTR was evaluated by immunohistochemistry. Results Our results showed that positive cells for both markers were present in all stages of tooth development, and the number of positive cells increased with the progression of this process. Cells of epithelial and ectomesenchymal origin were positive for CD146, and the expression of p75NTR was mainly detected in the dental papilla and dental follicle. In the postnatal group, dental pulp cells were positive for CD146, and the reduced enamel epithelium and the oral mucosa epithelium showed immunostaining for p75NTR. Conclusions These results suggest that the staining pattern of CD146 and p75NTR underwent temporal and spatial changes during odontogenesis and both markers were expressed by epithelial and mesenchymal cell types, which is relevant due to the significance of the epithelial-ectomesenchymal interactions in tooth development.


Subject(s)
Animals , Mice , Mesenchymal Stem Cells , Odontogenesis , Stem Cells , Cell Differentiation , Receptors, Nerve Growth Factor , CD146 Antigen , Mice, Inbred BALB C
2.
Braz. oral res. (Online) ; 34: e033, 2020. graf
Article in English | LILACS | ID: biblio-1089391

ABSTRACT

Abstract The aim of our study was to isolate populations of keratinocyte stem cells based on the expression of cell surface markers and to investigate whether the culture could affect their phenotype. keratinocytes from human oral mucosa were sorted based on the expression of the epithelial stem cell markers p75NTR and CD71. We also examined the co-expression of other epithelial stem markers such as integrins β1 and α6 and their stem cell-like proprieties in in vitro assays. Three passages after being sorted by MACS, more than 93% of the p75NTR+ve cells lost the expression of p75NTR, while 5.46% of the p75NTR-ve gained it. Within the small population of the p75NTR+ve cells, 88% co-expressed other epithelial stem cell markers such as integrins β1 and α6, while only 28% of p75NTR-ve cells co-expressed these markers. These results were confirmed by sorting cells by FACS. Additionally, when double staining was used for sorting cells, 99% of the p75NTR+veCD71-ve and 33% of the p75NTR-veCD71+ve cells expressed both integrins, but just one week after culture, only 1.74% of the p75NTR+veCD71-ve cells still expressed p75NTR and only 0.32% still expressed CD71. Similar results were obtained when co-culturing p75NTR+ve and p75NTR-ve populations before analysis. Our results suggest that phenotype changes may be part of an intrinsic cellular mechanism to conserve levels of protein expression as they may found in the human body. In addition, in vitro culture may not offer ideal conditions for epithelial stem cell maintenance due to phenotype changes under standard culture conditions.


Subject(s)
Humans , Phenotype , Stem Cells/cytology , Keratinocytes/cytology , Cell Culture Techniques/methods , Epithelial Cells/cytology , Mouth Mucosa/cytology , Receptors, Transferrin/analysis , Biomarkers/analysis , Antigens, CD/analysis , Cell Separation/methods , Reproducibility of Results , Receptors, Nerve Growth Factor/analysis , Flow Cytometry/methods , Nerve Tissue Proteins/analysis
3.
Article in English | WPRIM | ID: wpr-765398

ABSTRACT

OBJECTIVE: Nerve growth factor (NGF) is a member of the neurotrophic factor family and plays a vital role in the physiological processes of organisms, especially in the nervous system. Many recent studies have reported that NGF is also involved in the regulation of tumourigenesis by either promoting or suppressing tumor growth, which depends on the location and type of tumor. However, little is known regarding the effect of NGF on interspinal schwannoma (IS). In the present study, we aimed to explored whether mouse nerve growth factor (mNGF), which is widely used in the clinic, can influence the growth of interspinal schwannoma cells (ISCs) isolated from IS in vitro. METHODS: ISCs were isolated, cultured and identified by S-100 with immunofluorescence analysis. S-100-positive cells were divided into five groups, and separately cultured with various concentrations of mNGF (0 [phosphate buffered saline, PBS], 40, 80, 160, and 320 ng/mL) for 24 hours. Western blot and quantantive real time polymerase chain reaction (PCR) were applied to detect tyrosine kinase A (TrkA) receptor and p75 neurotrophin receptor (p75(NTR)) in each group. Crystal violet staining was selected to assess the effect of mNGF (160 ng/mL) on ISCs growth. RESULTS: ISCs growth was enhanced by mNGF in a dose-dependent manner. The result of crystal violet staining revealed that it was significantly strengthened the cells growth kinetics when cultured with 160 ng/mL mNGF compared to PBS group. Western blot and quantantive real time PCR discovered that TrkA receptor and mRNA expression were both up-regualated under the condition of mNGF, expecially in 160 ng/mL, while the exoression of p75(NTR) demonstrated no difference among groups. CONCLUSION: From these data, we conclude that exogenous mNGF can facilitate ISC growth by activating both TrkA receptor and p75(NTR). In addition, patients who are suffering from IS should not be administered mNGF in the clinic.


Subject(s)
Animals , Blotting, Western , Fluorescent Antibody Technique , Gentian Violet , Humans , In Vitro Techniques , Kinetics , Mice , Nerve Growth Factor , Nervous System , Neurilemmoma , Physiological Phenomena , Protein-Tyrosine Kinases , Real-Time Polymerase Chain Reaction , Receptor, Nerve Growth Factor , Receptor, trkA , Receptors, Nerve Growth Factor , RNA, Messenger
4.
Article in English | WPRIM | ID: wpr-788824

ABSTRACT

OBJECTIVE: Nerve growth factor (NGF) is a member of the neurotrophic factor family and plays a vital role in the physiological processes of organisms, especially in the nervous system. Many recent studies have reported that NGF is also involved in the regulation of tumourigenesis by either promoting or suppressing tumor growth, which depends on the location and type of tumor. However, little is known regarding the effect of NGF on interspinal schwannoma (IS). In the present study, we aimed to explored whether mouse nerve growth factor (mNGF), which is widely used in the clinic, can influence the growth of interspinal schwannoma cells (ISCs) isolated from IS in vitro.METHODS: ISCs were isolated, cultured and identified by S-100 with immunofluorescence analysis. S-100-positive cells were divided into five groups, and separately cultured with various concentrations of mNGF (0 [phosphate buffered saline, PBS], 40, 80, 160, and 320 ng/mL) for 24 hours. Western blot and quantantive real time polymerase chain reaction (PCR) were applied to detect tyrosine kinase A (TrkA) receptor and p75 neurotrophin receptor (p75(NTR)) in each group. Crystal violet staining was selected to assess the effect of mNGF (160 ng/mL) on ISCs growth.RESULTS: ISCs growth was enhanced by mNGF in a dose-dependent manner. The result of crystal violet staining revealed that it was significantly strengthened the cells growth kinetics when cultured with 160 ng/mL mNGF compared to PBS group. Western blot and quantantive real time PCR discovered that TrkA receptor and mRNA expression were both up-regualated under the condition of mNGF, expecially in 160 ng/mL, while the exoression of p75(NTR) demonstrated no difference among groups.CONCLUSION: From these data, we conclude that exogenous mNGF can facilitate ISC growth by activating both TrkA receptor and p75(NTR). In addition, patients who are suffering from IS should not be administered mNGF in the clinic.


Subject(s)
Animals , Blotting, Western , Fluorescent Antibody Technique , Gentian Violet , Humans , In Vitro Techniques , Kinetics , Mice , Nerve Growth Factor , Nervous System , Neurilemmoma , Physiological Phenomena , Protein-Tyrosine Kinases , Real-Time Polymerase Chain Reaction , Receptor, Nerve Growth Factor , Receptor, trkA , Receptors, Nerve Growth Factor , RNA, Messenger
5.
Braz. oral res. (Online) ; 30(1): e109, 2016. tab, graf
Article in English | LILACS | ID: biblio-952054

ABSTRACT

Abstract Multicystic and unicystic ameloblastomas are benign odontogenic tumors that present distinct biological behavior. The investigation of stem cells has become an important branch of tumor biology, with several studies addressing the possible role of these cells in tumor growth, angiogenesis, progression, infiltration and invasiveness. This study evaluated the immunohistochemical expression of CD90(Thy-1) and P75NTR stem cell markers in multicystic and unicystic ameloblastomas. Seventeen (17) samples of ameloblastomas (multicystic, n = 10; unicystic, n = 7) were submitted to immunohistochemical reactions and graded semi-quantitatively. The Kolmogorov-Smirnov test was used to verify possible differences in CD90 and P75NTR expressions between multicystic and unicystic ameloblastomas (p < 0.05). CD90 immunostaining was observed in all multicystic ameloblastoma specimens (n = 10), in the cytoplasm of the fibroblasts and vascular endothelial cells of the tumor stroma, near the neoplastic odontogenic epithelia. The staining of stromal CD90 was significantly higher in multicystic than in unicystic ameloblastomas (p = 0.003). Nuclear P75NTR immunostaining was observed in all ameloblastoma specimens. A significant difference was seen in the epithelial staining of P75NTR between multicystic and unicystic types (p = 0.007). The increased expression of CD90 and P75NTR found in multicystic ameloblastomas suggests a behavioral biological difference between multicystic and unicystic ameloblastomas, as well as a difference in ameloblastoma development.


Subject(s)
Humans , Male , Female , Adolescent , Adult , Young Adult , Neoplastic Stem Cells/metabolism , Ameloblastoma/metabolism , Mandibular Neoplasms/metabolism , Biomarkers, Tumor/metabolism , Receptors, Nerve Growth Factor/metabolism , Thy-1 Antigens/metabolism , Nerve Tissue Proteins/metabolism , Neoplastic Stem Cells/pathology , Immunohistochemistry , Ameloblastoma/pathology , Mandibular Neoplasms/pathology , Paraffin Embedding , Stromal Cells , Statistics, Nonparametric , Endothelial Cells/metabolism , Fibroblasts/metabolism , Middle Aged
6.
Braz. j. phys. ther. (Impr.) ; 19(3): 167-176, May-Jun/2015. tab
Article in English | LILACS | ID: lil-751381

ABSTRACT

Introduction: Patellofemoral pain syndrome (PFPS) is characterized by anterior knee pain, which may limit the performance of functional activities. The influence of hip joint motion on the development of this syndrome has already been documented in the literature. In this regard, studies have investigated the effectiveness of hip muscle strengthening in patients with PFPS. Objectives: The aims of this systematic review were (1) to summarize the literature related to the effects of hip muscle strengthening on pain intensity, muscle strength, and function in individuals with PFPS and (2) to evaluate the methodological quality of the selected studies. Method: A search for randomized controlled clinical trials was conducted using the following databases: Google Scholar, MEDLINE, PEDro, LILACS, and SciELO. The selected studies had to distinguish the effects of hip muscle strengthening in a group of patients with PFPS, as compared to non-intervention or other kinds of intervention, and had to investigate the following outcomes: pain, muscle strength, and function. The methodological quality of the selected studies was analyzed by means of the PEDro scale. Results: Seven studies were selected. These studies demonstrated that hip muscle strengthening was effective in reducing pain. However, the studies disagreed regarding the treatments' ability to improve muscle strength. Improvement in functional capabilities after hip muscle strengthening was found in five studies. Conclusion: Hip muscle strengthening is effective in reducing the intensity of pain and improving functional capabilities in patients with PFPS, despite the lack of evidence for its ability to increase muscle strength. .


Subject(s)
Animals , Female , Rats , Afferent Pathways/physiology , Muscle, Skeletal/physiology , Neuronal Plasticity/physiology , Nociception/physiology , Reflex/physiology , Skin/innervation , Analgesics, Non-Narcotic/pharmacology , Bupivacaine/pharmacology , Dexmedetomidine/pharmacology , Evoked Potentials, Somatosensory/drug effects , Evoked Potentials, Somatosensory/physiology , Muscle, Skeletal/drug effects , Neural Conduction/drug effects , Neuronal Plasticity/drug effects , Nociception/drug effects , Physical Stimulation/adverse effects , Rats, Sprague-Dawley , Receptors, Nerve Growth Factor/metabolism , Reflex/drug effects , Somatostatin/metabolism , Spinal Cord/drug effects , Spinal Cord/metabolism , Ubiquitin Thiolesterase/metabolism
7.
Arq. neuropsiquiatr ; 73(5): 420-424, 05/2015. tab, graf
Article in English | LILACS | ID: lil-746489

ABSTRACT

Neurotrophic factors (NF) are involved in pain regulation and a few studies have suggested that they may play a pathophysiological role in primary headaches. The aim of this study was to investigate NF levels in patients with tension type headache (TTH). We carried out a cross sectional study including 48 TTH patients and 48 age and gender matched controls. Beck Depression and Anxiety Inventories, and Headache Impact Test were recorded. Serum levels of NF were determined by ELISA. There were not significant differences between NF levels between TTH patients and controls. Patients with chronic and episodic TTH had not significant differences in NF levels. The presence of headache at the time of evaluation did not significantly alter the levels of NF. Depression and anxiety scores as well as headache impact did not correlate with NF levels. Our study suggest that the serum levels of NF are not altered in TTH.


Os fatores neurotróficos (FN) participam da regulação da dor e podem ter um papel na fisiopatologia das cefaleias peimárias. O objetivo do presente estudo foi avaliar os níveis séricos de FN em pacientes com cefaleia do tipo tensional (CTT). Foi realizado corte transversal com 48 pacientes com CTT e 48 controles pareados por gênero e idade. Os inventários de Beck para depressão e ansiedade, bem como o inventário de impacto da cefaleia foram aplicados. Os níveis séricos de FN foram determinados por ELISA. Não houve diferenças significativas entre níveis de FN entre pacientes com TTH e controles, bem como entre pacientes com TTH episódica e crônica. Presença de cefaleia no momento da avaliação não alterou os níveis séricos de FN. Os escores de depressão, ansiedade e impacto da cefaleia não se correlacionaram com os níveis de FN. Nosso estudo sugere que não há alteração dos níveis de FN na TTH.


Subject(s)
Adult , Female , Humans , Male , Middle Aged , Young Adult , Nerve Growth Factors/blood , Tension-Type Headache/blood , Anxiety/blood , Anxiety/physiopathology , Case-Control Studies , Cross-Sectional Studies , Depression/blood , Depression/physiopathology , Enzyme-Linked Immunosorbent Assay , Psychometrics , Reference Values , Receptors, Nerve Growth Factor/blood , Severity of Illness Index , Statistics, Nonparametric , Tension-Type Headache/physiopathology
8.
Article in English | WPRIM | ID: wpr-290152

ABSTRACT

Mesenchymal stem cells (MSCs) are a promising tool in regenerative medicine due to their capacity to differentiate into multiple lineages. In addition to MSCs isolated from bone marrow (BMSCs), adult MSCs are isolated from craniofacial tissues including dental pulp tissues (DPs) using various stem cell surface markers. However, there has been a lack of consensus on a set of surface makers that are reproducibly effective at isolating putative multipotent dental mesenchymal stem cells (DMSCs). In this study, we used different combinations of surface markers (CD51/CD140α, CD271, and STRO-1/CD146) to isolate homogeneous populations of DMSCs from heterogeneous dental pulp cells (DPCs) obtained from DP and compared their capacity to undergo multilineage differentiation. Fluorescence-activated cell sorting revealed that 27.3% of DPCs were CD51(+)/CD140α(+), 10.6% were CD271(+), and 0.3% were STRO-1(+)/CD146(+). Under odontogenic conditions, all three subsets of isolated DMSCs exhibited differentiation capacity into odontogenic lineages. Among these isolated subsets of DMSCs, CD271(+) DMSCs demonstrated the greatest odontogenic potential. While all three combinations of surface markers in this study successfully isolated DMSCs from DPCs, the single CD271 marker presents the most effective stem cell surface marker for identification of DMSCs with high odontogenic potential. Isolated CD271(+) DMSCs could potentially be utilized for future clinical applications in dentistry and regenerative medicine.


Subject(s)
Adult , Adult Stem Cells , Cell Biology , Antigens, CD , Antigens, Surface , Biomarkers , CD146 Antigen , Cell Culture Techniques , Cell Differentiation , Physiology , Cell Lineage , Cell Separation , Methods , Cells, Cultured , Chondrogenesis , Physiology , Dental Pulp , Cell Biology , Flow Cytometry , Methods , Humans , Integrin alphaV , Mesenchymal Stem Cells , Cell Biology , Multipotent Stem Cells , Cell Biology , Nerve Tissue Proteins , Odontogenesis , Physiology , Receptor, Platelet-Derived Growth Factor alpha , Receptors, Nerve Growth Factor
9.
Article in English | WPRIM | ID: wpr-290151

ABSTRACT

Mesenchymal stem cell (MSC)-mediated therapy has been shown to be clinically effective in regenerating tissue defects. For improved regenerative therapy, it is critical to isolate homogenous populations of MSCs with high capacity to differentiate into appropriate tissues. The utilization of stem cell surface antigens provides a means to identify MSCs from various tissues. However, few surface markers that consistently isolate highly regenerative MSCs have been validated, making it challenging for routine clinical applications and making it all the more imperative to identify reliable surface markers. In this study, we used three surface marker combinations: CD51/CD140α, CD271, and STRO-1/CD146 for the isolation of homogenous populations of dental mesenchymal stem cells (DMSCs) from heterogeneous periodontal ligament cells (PDLCs). Fluorescence-activated cell sorting analysis revealed that 24% of PDLCs were CD51(+)/CD140α(+), 0.8% were CD271(+), and 2.4% were STRO-1(+)/CD146(+). Sorted cell populations were further assessed for their multipotent properties by inducing osteogenic and chondrogenic differentiation. All three subsets of isolated DMSCs exhibited differentiation capacity into osteogenic and chondrogenic lineages but with varying degrees. CD271(+) DMSCs demonstrated the greatest osteogenic potential with strong induction of osteogenic markers such as DLX5, RUNX2, and BGLAP. Our study provides evidence that surface marker combinations used in this study are sufficient markers for the isolation of DMSCs from PDLCs. These results provide important insight into using specific surface markers for identifying homogenous populations of DMSCs for their improved utilization in regenerative medicine.


Subject(s)
Adaptor Proteins, Signal Transducing , Adult , Aggrecans , Antigens, CD , Antigens, Surface , CD146 Antigen , Cell Differentiation , Physiology , Cell Lineage , Cell Separation , Methods , Cells, Cultured , Chondrogenesis , Physiology , Collagen Type II , Core Binding Factor Alpha 1 Subunit , Flow Cytometry , Methods , Homeodomain Proteins , Humans , Integrin alphaV , Mesenchymal Stem Cells , Cell Biology , Physiology , Multipotent Stem Cells , Cell Biology , Physiology , Nerve Tissue Proteins , Osteogenesis , Physiology , Periodontal Ligament , Cell Biology , Receptor, Platelet-Derived Growth Factor alpha , Receptors, Nerve Growth Factor , SOX9 Transcription Factor , Time Factors , Transcription Factors
10.
National Journal of Andrology ; (12): 982-987, 2015.
Article in Chinese | WPRIM | ID: wpr-304786

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the expressions and action mechanisms of nerve growth factor (NGF) receptors TrkA and p75NTR in the oncogenesis and progression of prostate cancer (PCa).</p><p><b>METHODS</b>Using immunohistochemistry, we detected the expressions of TrkA and p75NTR in 62 PCa and 35 benign prostatic hyperplasia (BPH) samples, and conducted statistical analysis on the basis of clinical data.</p><p><b>RESULTS</b>Independent-samples t-test showed that, along with poorer tissue differentiation or higher clinical stage of PCa, the expression of TrkA was significantly up-regulated, that of p75NTR remarkably down-regulated, and the expression ratio of TrkA to p75NTR markedly increased. The TrkA/p75NTR ratio was 0.32 in the BPH, 0.52 in the PCa tissue with Gleason score of 6, 1.65 in the PCa tissue with Gleason score of 7, 5.75 in the PCa tissue with Gleason score ≥ 8, 0.89 in the clinical stage of pT2, 1.5 in pT3 a, 3.75 in pT3b, and 7.00 in pTxN1.</p><p><b>CONCLUSION</b>The abnormally increased expression ratio of TrkA to p75NTR might be one of the essential features of malignant transformation of prostate cells. A higher TrkA/p75NTR expression ratio may be associated with a lower tissue differentiation, a higher clinical stage or Gleason score, and therefore a poorer prognosis.</p>


Subject(s)
Humans , Immunohistochemistry , Male , Neoplasm Grading , Neoplasm Staging , Nerve Tissue Proteins , Metabolism , Prognosis , Prostatic Hyperplasia , Pathology , Prostatic Neoplasms , Pathology , Receptor, trkA , Metabolism , Receptors, Nerve Growth Factor , Metabolism , Up-Regulation
11.
Article in Chinese | WPRIM | ID: wpr-315883

ABSTRACT

<p><b>OBJECTIVE</b>To study the biological characteristics of p75 neurotrophin receptor positive (p75(NTR+)) tongue squamous cell carcinoma cells which were separated by flow cytometry cell sorting.</p><p><b>METHODS</b>To determine the biological characteristics of p75(NTR+) cells which were separated from Tca-8113 and Cal-27 tongue squamous cell carcinoma cells by flow cytometry cell sorting, including study the capacity of cloning, 3-(4,5)-demethylthiazo(z-y1)-3,5-diphenytetrazoliumromide (MTT) assay, wound healing assay. p75(NTR+) cells with non-sorted cells were as control group.</p><p><b>RESULTS</b>In Tca-8113 and Cal-27 tongue squamous cell carcinoma cell lines, the percentage of p75(NTR+) cells were 3.1% and 1.9%. Compared with p75(NTR+) cells with non-sorted cells, p75(NTR+) cells possess higher capacity of cloning (Tca-8113, P=0.024; Cal-27, P=0.009). The percentage of p75(NTR+) cells of the progeny cells generated from monoclonal p75(NTR+) cells decreased to 14.5% (Tca-8113) and 5.8% (Cal-27) after cultured two weeks. p75(NTR+) cells possessed higher proliferation ability and higher metastasis ability than non-sorted cells.</p><p><b>CONCLUSION</b>p75(NTR+) cells isolated from tongue squamous cell carcinoma have the characteristics of cancer stem cells.</p>


Subject(s)
Carcinoma, Squamous Cell , Humans , Neoplasms , Nerve Tissue Proteins , Receptors, Nerve Growth Factor , Tongue Neoplasms
12.
Journal of Gorgan University of Medical Sciences. 2014; 16 (1): 35-41
in Persian | IMEMR | ID: emr-157571

ABSTRACT

Trophic factor family plays a key role for neuromuscular system healthy. This study was carried out to determine the effect of one session of resistance exercise on protein content and mRNA expression of NT4/5 in rat slow and fast muscles. In this experimental study, sixteen adult male rats randomly were allocated into resistance exercise [T] and control groups. The resistance training protocol consisted of climbing a 1-meter-long ladder, with a weight attached to a tail sleeve. Quantitative Real time RT-PCR for NT-4/5 expression and ELISA Kit for protein assay were used. Resistance training significantly decreased mRNA expression and increased protein of NT4/5 in soleus muscle [P<0.05]. Significant alteration was not detected in flexor hallucis longus muscle. One session of resistance training can alter protein and mRNA of NT-4/5 in skeletal muscle and this alteration was dependent on muscle type


Subject(s)
Animals, Laboratory , Male , Muscle, Skeletal/metabolism , RNA, Messenger/metabolism , Receptors, Nerve Growth Factor , Rats , Random Allocation , Enzyme-Linked Immunosorbent Assay , Reverse Transcriptase Polymerase Chain Reaction , Physical Conditioning, Animal
13.
Mem. Inst. Oswaldo Cruz ; 107(supl.1): 68-73, Dec. 2012. ilus, tab
Article in English | LILACS | ID: lil-659743

ABSTRACT

It is important to understand the mechanisms that enable peripheral neurons to regenerate after nerve injury in order to identify methods of improving this regeneration. Therefore, we studied nerve regeneration and sensory impairment recovery in the cutaneous lesions of leprosy patients (LPs) before and after treatment with multidrug therapy (MDT). The skin lesion sensory test results were compared to the histopathological and immunohistochemical protein gene product (PGP) 9.5 and the p75 nerve growth factor receptors (NGFr) findings. The cutaneous neural occupation ratio (CNOR) was evaluated for both neural markers. Thermal and pain sensations were the most frequently affected functions at the first visit and the most frequently recovered functions after MDT. The presence of a high cutaneous nerve damage index did not prevent the recovery of any type of sensory function. The CNOR was calculated for each biopsy, according to the presence of PGP and NGFr-immunostained fibres and it was not significantly different before or after the MDT. We observed a variable influence of MDT in the recovery from sensory impairment in the cutaneous lesions of LPs. Nociception and cold thermosensation were the most recovered sensations. The recovery of sensation in the skin lesions appeared to be associated with subsiding inflammation rather than with the regenerative activity of nerve fibres.


Subject(s)
Adolescent , Adult , Aged , Child , Female , Humans , Male , Middle Aged , Young Adult , Leprosy/physiopathology , Nerve Regeneration/physiology , Peripheral Nervous System Diseases/physiopathology , Receptors, Nerve Growth Factor/physiology , Immunohistochemistry , Leprostatic Agents/therapeutic use , Leprosy/drug therapy , Leprosy/pathology , Peripheral Nervous System Diseases/pathology , Sensory Thresholds , Thermosensing
14.
Article in Chinese | WPRIM | ID: wpr-746771

ABSTRACT

OBJECTIVE@#To study the expression of PCNA and LNGFr in olfactory epithelium of patients suffering from dysosmia caused by chronic sinusitis, and the function of LNGFr.@*METHOD@#Forty-six patients undergoing FESS were chosen. Before operation, their olfactory functions were examined with CCCRC. According to their CCCRC scores, they were divided into three groups. Group A: Patients with chronic sinusitis and dysosmia 25 cases; Group B: Patients with chronic sinusitis and a normal olfactory function 10 cases; Group C: Patients with deviation of nasal septum and a normal olfactory function 11 cases. The expressions of PCNA and LNGFr were measured in olfactory mucosas of the three groups by immunohistochemistry.@*RESULT@#In basal cells, the expression of PCNA and LNGFr in group A was higher than that in group B (P < 0.01). and in group C (P < 0.01). There was negative correlation between positive cells of PCNA and CCCRC score in basal cells of group A (r = -0.7441, P < 0.01); There was negative correlation between integral optical density of LNGFr and CCCRC score in basal cells of group A (r = -0.4407, P < 0.05). There was positive correlation between positive cells of PCNA and integral optical density of LNGFr in basal cells of group A (r = 0.5317, P < 0.01).@*CONCLUSION@#Basal cells proliferated dramatically in patients suffering from dysosmia caused by chronic sinusitis. The proliferating capacity of basal cells was related to up-regulation of LNGFr expression.


Subject(s)
Chronic Disease , Humans , Immunohistochemistry , Nerve Tissue Proteins , Metabolism , Olfaction Disorders , Metabolism , Olfactory Mucosa , Metabolism , Proliferating Cell Nuclear Antigen , Metabolism , Receptors, Nerve Growth Factor , Metabolism , Sinusitis
15.
Braz. j. med. biol. res ; 44(7): 682-687, July 2011. ilus, tab
Article in English | LILACS | ID: lil-595697

ABSTRACT

We investigated the reactivity and expression of basal lamina collagen by Schwann cells (SCs) cultivated on a supraorganized bovine-derived collagen substrate. SC cultures were obtained from sciatic nerves of neonatal Sprague-Dawley rats and seeded on 24-well culture plates containing collagen substrate. The homogeneity of the cultures was evaluated with an SC marker antibody (anti-S-100). After 1 week, the cultures were fixed and processed for immunocytochemistry by using antibodies against type IV collagen, S-100 and p75NTR (pan neurotrophin receptor) and for scanning electron microscopy (SEM). Positive labeling with antibodies to the cited molecules was observed, indicating that the collagen substrate stimulates SC alignment and adhesion (collagen IV labeling - organized collagen substrate: 706.33 ± 370.86, non-organized collagen substrate: 744.00 ± 262.09; S-100 labeling - organized collagen: 3809.00 ± 120.28, non-organized collagen: 3026.00 ± 144.63, P < 0.05) and reactivity (p75NTR labeling - organized collagen: 2156.33 ± 561.78, non-organized collagen: 1424.00 ± 405.90, P < 0.05; means ± standard error of the mean in absorbance units). Cell alignment and adhesion to the substrate were confirmed by SEM analysis. The present results indicate that the collagen substrate with an aligned suprastructure, as seen by polarized light microscopy, provides an adequate scaffold for SCs, which in turn may increase the efficiency of the nerve regenerative process after in vivo repair.


Subject(s)
Animals , Cattle , Rats , Collagen Type IV/metabolism , Extracellular Matrix/metabolism , Nerve Regeneration/physiology , Receptors, Nerve Growth Factor/analysis , /analysis , Schwann Cells/metabolism , Cell Polarity , Cell Shape , Cells, Cultured , Collagen Type IV/analysis , Immunohistochemistry , Materials Testing , Microscopy, Electron, Scanning , Polymers/chemistry , Rats, Sprague-Dawley , Receptors, Nerve Growth Factor/immunology , /immunology , Sciatic Nerve , Staining and Labeling , Schwann Cells/cytology
16.
Journal of Experimental Hematology ; (6): 1273-1276, 2011.
Article in Chinese | WPRIM | ID: wpr-261885

ABSTRACT

The aim of this study was to detect the nerve growth factor (NGF) level in serum and NGF low affinity acceptor CD271 expression on bone marrow leukemic cells in acute B lymphoid leukemia (B-ALL) patients and to analyze their clinical significance. The NGF level in serum and CD271 expression on leukemic cells in bone marrow were detected by enzyme linked immunosorbent assay and flow cytometry in B-ALL patients respectively. The results indicated that compared with control group, the NGF level in serum of patient group significantly increased (t = 4.191, p < 0.05), but CD271 expression on leukemic cells in bone marrow decreased significantly (t = 4.898, p < 0.05). The complete remission (CR) rate of 25 B-ALL patients was 64% (16/25) after one course of CVAD chemotherapy. There were statistically significant differences of NGF level and CD271 expression in non-remission (NR) group and control group (t = 3.976, p < 0.05 vs t = 5.052, p < 0.05), but there were no statistically difference of NGF level and CD271 expression in CR group (t = 1.102, p > 0.05 vs t = 1.150, p > 0.05) as compared with control group. The CD271 expression before and after chemotherapy between CR and NR groups showed statistically significant differences (t = 3.889, p < 0.05; t = 3.751, p < 0.05 and t = 4.678, p < 0.05 respectively), but NGF level before and after chemotherapy showed no statistical difference between these 2 groups (t = 0.476, p > 0.05). 50% (8/16) patients relapsed during following up, and of their NGF level [(168.00 ± 61.66) pg/ml] and CD271 expression [(52.29 ± 13.00)%] showed the significantly differences, compared with those in control group (t = 5.284, p < 0.05 vs. t = 6.073, p < 0.05), but the NGF level [(81.13 ± 25.32) pg/ml] and CD271 expression [(78.45 ± 7.12)%] of other 8 patients showed no statistical difference as compared with control group (t = 1.228, p > 0.05 vs t = 1.144, p > 0.05). Compared with low NGF level and CD271 low expression groups, the survival time of B-ALL patients with high NGF level and CD271 expression was not changed significantly (p = 0.750 vs p = 0.170). It is concluded that the increased NGF level in serum and decreased CD271 expression on bone marrow leukemic cells in B-ALL patients are related with leukemia development and may be the useful indexes to evaluate curative effect and prognosis.


Subject(s)
Adolescent , Adult , Aged , Bone Marrow Cells , Metabolism , Case-Control Studies , Child , Child, Preschool , Female , Humans , Male , Middle Aged , Nerve Growth Factor , Blood , Nerve Tissue Proteins , Metabolism , Precursor Cell Lymphoblastic Leukemia-Lymphoma , Blood , Metabolism , Receptors, Nerve Growth Factor , Metabolism , Young Adult
17.
J. epilepsy clin. neurophysiol ; 16(1): 7-12, 2010. graf, tab
Article in Portuguese | LILACS | ID: lil-548916

ABSTRACT

INTRODUÇÃO: A neurotrofinas NGF, BDNF, NT-3 e NT-4 são os principais representantes da família das neurotrofinas no sistema nervoso central de mamíferos. Estão presentes em estágios específicos do crescimento e sobrevivência neuronal como a divisão celular, diferenciação e axogênese e também nos processos naturais de morte celular neuronal. A atividade biológica das neurotrofinas é mediada pelos receptores de tropomiosina quinase Trk. NGF ativa principalmente os receptores TrkA, BDNF e NT-4 interagem com os receptores TrkB e NT-3 com TrkC. Todas as NTs também podem se ligar, com menor afinidade, ao receptor p75NTR. Nesta breve revisão serão levantadas as principais evidências sobre o papel e expressão das principais neurotrofinas no hipocampo, com ênfase nas alterações que ocorrem em modelos animais de epilepsia. RESULTADOS: As neurotrofinas parecem ter um papel chave na plasticidade sináptica relacionada à epilepsia, onde elas poderiam agir tanto como fatores promotores da epileptogênese quanto como substâncias anti-epiléptogênicas endógenas. Além disso a expressão dos genes que codificam os fatores neurotróficos e seus receptores pode ser alterada pela atividade de crises em diversos modelos de epilepsia. CONCLUSÃO: Vários estudos têm demonstrado a relação entre a expressão das neurotrofinas e as alterações na plasticidade dos circuitos neuronais que ocorrem após danos cerebrais, tais como a epilepsia. O conhecimento das alterações na expressão das neurotrofinas na plasticidade neuronal pode nos auxiliar a entender como estas moléculas participam dos mecanismos epileptogênicos e dessa forma, dar início ao estudo de novas terapias e ao desenvolvimento de novas drogas que auxiliem no tratamento da epilepsia.


INTRODUCTION: NGF, BDNF, NT-3 and NT-4 are the major neurotrophins in the mammal central nervous system. These proteins play key roles in development of the nervous system, but they are also responsible for important functions in the adult brain, such as trophic support of adult neurons, cell plasticity and death. The neurotrophins activate three different members of the tropomyosin-related kinase (Trk) family of receptor tyrosine kinases. These three receptors exhibit distinct affinities for different neurotrophins, with NGF activating TrkA, BDNF and NT-4 activating TrkB, and NT-3 predominantly activating TrkC. All NTs can also interact with the receptor p75NTR, a member of the tumor necrosis factor receptor superfamily. RESULTS: NTs have a key role also in the neuronal plasticity related to epilepsy, and they are able to act as epileptogenic factors and anti-epileptogenic endogenous factors. Besides that, several studies have shown that status epilepticus and chronic seizures may alter gene and protein expression of these factors. CONCLUSION: Here, we briefly give a short review of current knowledge of the roles and expression of the major neurotrophins in the hippocampus, with emphasis to the changes that occur in animal models of epilepsy. The knowledge on how the mechanisms underlying the multiplicity of biological functions in which the neurotrophins take part may provide us key insights into the cellular mechanisms of neuronal function in health and disease.


Subject(s)
Receptors, Nerve Growth Factor , Epilepsy, Temporal Lobe , Nerve Growth Factors
18.
Rev. arg. morfol ; 1(2): 6-6, 2010.
Article in Spanish | LILACS | ID: lil-708276

ABSTRACT

Las neurotrofinas (NTs) son factores de crecimiento que ejercen la totalidad o parte de sus funciones sobreel sistema nervioso central y periférico. Las neurotrofinas son una familia de péptidos con propiedades neurotróficas y neurotrópicas que incluyen los siguientes compuestos: factor de crecimiento nerviosos, (nerve growth factor, NGF), factor de crecimiento derivado del cerebro (Brain-derived neurotrophic factor, BDNF), y las neurotrofinas 3 (NT-3), 4/5 (NT/4/5), (NT-6). Se trata de un conjunto de péptidos relacionados estructural yfuncionalmente, que favorecen la supervivencia y diferenciación fenotípica de su-poblaciones neuronales del sistemanervioso periférico durante el desarrollo embrionario y su mantenimiento durante la vida adulta. Además actúansobre algunos tipos de neuronas del sistema nervioso central. Por otro lado, estudios llevados a cabo en los últimosaños sugieren que las neurotrofinas podrían controlar algunas funciones en tejidos no nerviosos, especialmente enlos órganos linfoides y en las células inmunocompetentes. En medicina Humana se hadeterminado que en el aparato de la visión la falta de BDNF lleva: 1) a la pérdida de las células ganglionares de laretina y también es responsable del glaucoma en el hombre; 2) En el Sistema nervios causa depresión, epilepsia, enla enfermedad de Huntington; .3) y en la parte metabólica causa la diabetes tipo. Está comprobado que las dosprimeras (NTs) están presentes en el carcinoma de colon, páncreas y glándulas prostáticas, para estas dos patologíasya están en venta la anti-neurotrofinas que neutralizan a los NGF, BDNF, NT-3, NT 4/5NT e incluyen anticuerposgenerados así como sus fragmentos.En Medicina veterinaria se han detectados a los receptores de alta afinidad(Proteínas Trks) en el timo de todos los vertebrados inferiores y superiores. También en los órganos linfáticossecundarios de Sus scrofa domesticus y Lama glama; en el aparato digestivo y reproductor de Rattus norvergicus.


Neurotrophins (NTs ) are growth factors that exert all or part of its functions onthe central and peripheral nervous system. Neurotrophins are a family of peptides with neurotrophic and neurotropic properties include the following compounds: nerve growth factor ( nerve growth factor , NGF) , growth factor , brain derived ( Brain- derived neurotrophic factor , BDNF) , and neurotrophin 3 (NT -3 ) , 4 /5 ( NT/4/5 ) , ( NT - 6) . This is a set of structural and related peptides functionally , favoring survival and phenotypic differentiation of neuronal populations system su-peripheral nervous during embryonic development and maintenance during adulthood . Besides actingon some types of neurons of the central nervous system . Moreover, studies conducted in recentyears suggest that neurotrophins may control some functions in non-nervous tissues, especially inand lymphoid organs in immunocompetent cells . In Human medicine has determined that in the apparatus of the absence of BDNF vision leads : 1) a loss of ganglion cellsretina and is also responsible for glaucoma in man; 2) In the nerve system causes depression, epilepsy,Huntington 's disease ; 3 ) and in metabolic diabetes causes type part . It is proved that the twofirst (NTs ) are present in the carcinoma of the colon, pancreas and prostate glands , for these two pathologies Information already in the anti - neurotrophins that neutralize NGF , BDNF , NT -3 , NT and include antibodies 4/5NTgenerated and their fragmentos.En Veterinary Medicine detected to have high affinity receptors (Proteins Trks ) in the thymus of all lower and higher vertebrates. Also in the lymphatic organsHis side scrofa domesticus and Lama glama ; in the digestive tract and Rattus norvegicus.


Subject(s)
Animals , Nerve Growth Factors/classification , Nerve Growth Factors , Receptors, Nerve Growth Factor
19.
Chinese Medical Journal ; (24): 2695-2700, 2010.
Article in English | WPRIM | ID: wpr-285762

ABSTRACT

<p><b>BACKGROUND</b>By unbiased stereological methods, we have observed preferential dorsal root ganglion (DRG) B-cell loss in rodents after nerve injury, and caspase-3 activation and cell loss were related to the present of p75 receptor (p75(NTR)). We hypothesized that DRG B-cells express higher levels of pro-apoptotic proteins as compared to A-cells and the expressions of pro-apoptotic proteins can be reduced by depletion of p75(NTR). This study aimed to identify the p75(NTR) involved apoptotic pathway in DRG neurons after nerve injury.</p><p><b>METHODS</b>The p75(NTR) knockout mice (p75-/-) and wildtype Balb/C mice (p75+/+) were used in this study. The expressions of pro-apoptotic proteins, c-Jun-N-terminal kinase (JNK), c-jun and p38 in DRG were evaluated with immunohistochemistry 2 and 7 days following unilateral sciatic nerve transection. In addition, extra-cellular related kinase (ERK), a transducer of survival signals, was also tested with immunohistochemistry and Western blotting methods in these animal models.</p><p><b>RESULTS</b>Phosphorylated JNK (P-JNK) and phosphorylated p38 (P-p38) were mainly located in small B-cells, whereas phosphorylated c-jun (P-c-jun) was located in both A- and B-cells. Phosphorylated ERK (P-ERK) was located in both B-cells and satellite cells. Axotomy dramatically increased the expressions of P-JNK and P-c-jun (paired t-test), with no influence on the expressions of P-p38 and P-ERK. Furthermore, the increase of P-JNK in p75+/+ mice 2 days after nerve axotomy was approximately 2.2-folds of that in p75-/- mice (P = 0.001, unpaired t-test).</p><p><b>CONCLUSION</b>p75(NTR)-dependent JNK-caspase-3 pathway is involved in DRG B-cell loss after nerve injury and JNK is not the unique upstream of c-jun activation.</p>


Subject(s)
Animals , Apoptosis , Genetics , Physiology , Axotomy , Blotting, Western , Ganglia, Spinal , Cell Biology , Immunohistochemistry , Male , Mice , Mice, Inbred BALB C , Mice, Knockout , Neurons , Cell Biology , Metabolism , Receptors, Nerve Growth Factor , Genetics , Metabolism , Sciatic Nerve , General Surgery
20.
Article in Chinese | WPRIM | ID: wpr-246649

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the role of p75 neurotrophin receptor (p75NTR) in the regeneration of facial nerve crush injury.</p><p><b>METHODS</b>In p75NTR knockout mice and wild type mice, the regenerating fibres in the facial nerve were also labelled by an anterograde tracer cholera toxin B (CTB). The next day after injury of facial nerve, CTB was injected into the trunk of the nerve in the proximal side of the crush, and then anterograde tracing and immunohistochemistry were used to examine the regeneration of axons after facial nerve crush injury. In p75NTR knockout mice and wild type mice, the facial nerves on one side were crushed and regenerating neurons in the facial nerve nucleus were labelled by Fast Blue. The facial nerve trunk was cut in the bifurcated region in the 4th day after injury and the stump was inserted into a small polymer tube containing Fast Blue. Retrograde tracing and labling motoneuron counting were used to examine the survival of motoneurons in the facial nerve nucleus after facial nerve crush injury.</p><p><b>RESULTS</b>The results showed that the axonal growth of injured axons in the facial nerve of p75NTR knockout mice was significantly retarded. The number of regenerated neurons in the facial nerve nucleus in p75NTR knockout mice was significantly reduced (P < 0.05). Immunohistochemical staining of regenerating axons also showed the reduction in nerve regeneration in p75NTR knockout mice (P < 0.01).</p><p><b>CONCLUSION</b>p75NTR plays an important role in the regeneration of injured peripheral nerves after injury.</p>


Subject(s)
Animals , Axons , Facial Nerve , Mice , Motor Neurons , Nerve Regeneration , Neurons , Receptor, Nerve Growth Factor , Receptors, Nerve Growth Factor
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