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Article in Chinese | WPRIM | ID: wpr-878435


Secretory carcinoma, a low-grade malignant tumor, occurs mainly in parotid, submandibular gland, and small salivary glands in the mouth. It has not yet been reported in the mandible. Now we report a case occurred in the right mandibular angle of secretory carcinoma, accompanying with its diagnosis, treatment and prognosis.

Carcinoma , Humans , Mandible , Parotid Gland , Salivary Gland Neoplasms , Salivary Glands
Article in English | WPRIM | ID: wpr-878416


Oral squamous cell carcinoma (OSCC) is the most frequent tumour in head and neck malignant. The current treatment is mainly based on surgery therapy, radiation therapy and chemical therapy. Meanwhile, there are many a defect in the treatment. For example, there are many defects in radiotherapy. Radioactive salivatitis is the most common. In addition, there are a series of changes such as dry mouth, oral mucositis, rampant dental caries, and radioactive osteomyelitis of jaw, which cause swallowing, chewing problems, and taste dysfunction. Currently, the research on radioactive salivatitis is progressing rapidly, but its mechanism is more complication. This paper review aims to summarize the research progress in this field.

Carcinoma, Squamous Cell , Dental Caries , Head and Neck Neoplasms/radiotherapy , Humans , Mouth Neoplasms , Radiation Injuries , Salivary Glands , Xerostomia/etiology
Braz. dent. sci ; 24(4, suppl 1): 1-13, 2021. tab, ilus
Article in English | LILACS, BBO | ID: biblio-1349352


Objective: Head and neck cancer is the sixth leading cancer by incidence worldwide and eighth by death. Recent reports revealed that, not only radiotherapy but also chemotherapy may induce xerostomia. The aim of this study was to compare the possible regenerative effect of BMSCs through systemic and local injections. Material and Methods: 52 male Albino rats were randomly divided into 4 groups: Group 1: 10 rats received 0.5 ml of PBS by injection. Group 2: 14 rats received an intraperitoneal injection of 5-FU drug. Group 3: 14 rats were injected the same dose of 5-FU then received an intraglandular transplantation of BMSCs suspended in 0.5 ml of PBS at day 1 after 5-FU administration. Group 4: 14 rats were injected the same dose of 5-FU then received an intravenous injection of BMSCs suspended in 0.5 ml of PBS via the tail vein at day 1 after 5-FU administration. Results: Histological examination showed that group 2 showed features of severe degenerative changes which increased over time. Group 3 showed increasing amelioration in the ductal structure overtime. Group 4 also showed regenerated ductal elements however concerning apoptotic changes, immunohistochemistry results revealed improvement in both group 3 and 4 over group 2 with no statistical difference between groups 3 and 4. Conclusion: Histological and immunohistochemical features in group 3 and group 4 revealed similar amelioration in regenerative potentials. On the other hand, regenerative features of both experimental groups were statistically significant as compared independently to group 1 (AU)

Objetivo: O carcinoma de cabeça e pescoço é o sexto câncer de maior incidência no mundo sendo a oitava causa de morte por cancer. Relatos recentes revelaram que não apenas a radioterapia, mas também a quimioterapia podem induzir xerostomia. O objetivo desse estudo foi comparar a possivel ação regenerative de BMSCs através de injeção local e sistêmica. Material e Métodos: 52 ratos Albino foram aleatoriamente alocados em 4 grupos: Grupo 1: 10 ratos que receberam 0.5 ml de injeção de PBS. Grupo 2: 14 ratos que receberam injeção intraperitoneal da droga 5-FU. Grupo 3: 14 ratos que foram injetados com a mesma dose de 5-FU e receberam transplante intraglandular de BMSCs ressuspendidas em 0.5mL de PBS no dia 1 após a administração do 5-FU. Grupo 4: 14 ratos que foram injetados com a mesma dose de 5-FU e receberam injeção intravenosa de BMSCs ressuspendidas em 0.5mL de PBS via veia caudal 1 dia após a administração de 5-FU. Resultados: O exame histológico demonstrou que o grupo 2 apresentou alterações degenerativas severas que se agravaram com o tempo. O Grupo 3 mostrou melhora da estrutura ductal ao longo do experimento. Group 4 também mostrou elementos ductais regenerados. Referente a alterações apoptóticas,análise imunohistoquimica mostrou melhora nos grupos 3 e 4 comparados ao grupo 2, sendo que os grupos 3 e 4 foram estatisticamente semelhantes. Conclusão: Análises histológicas e imunohistoquímicas mostram que os grupos 3 e 4 apresentam melhora no potencial regenerativo Por outro lado, os resultados observados para os dois grupos foi estatisticamente semlhante quando comparados independentemente ao grupo 1 (AU)

Animals , Rats , Salivary Glands , Mesenchymal Stem Cells , Squamous Cell Carcinoma of Head and Neck
Adv Rheumatol ; 61: 8, 2021. tab, graf
Article in English | LILACS | ID: biblio-1152738


Abstract Background: Sjögren's Syndrome compromises the exocrine function, producing xerostomia and xerophthalmia. It can appear as an isolated condition or associated with other autoimmune diseases (polyautoimmunity). The Unstimulated Salivary Flow rate (UWSF) is used to quantify saliva production. There is no objective evidence to differentiate the values in patients with Sjögren's versus healthy people or patients with non-Sjögren's sicca. The objective of the present review was to evaluate the UWSF in patients with Sjögren's syndrome in comparison to controls (healthy and non-Sjögren's sicca patients). Methods: A systematic literature review was carried out (PRISMA guidelines). Analytical observational studies of cases and controls, cross-sectional studies, cohort studies and randomized clinical trials (including healthy controls) were considered. The Medline/OVID, Lilacs, Embase, and Cochrane/OVID databases were consulted. MeSH, DeCS, keywords, and Boolean operators were used. The meta-analysis (RevMan 5.2) was done through the random-effects model [mean difference (MD)]. Level and quality of evidence were evaluated by the Oxford Center Levels of Evidence and Joanna Brigs list respectively. Results: Thirty-two articles were included (20 were case-control studies,6 were cross-sectional,2 prospective cohort,2 retrospective cohort, and2 studies were abstracts) and 28 were meta-analyzed. The unstimulated whole salivary flow rate in the Sjögren's group was lower than in controls (healthy and patients with non-Sjögren Sicca syndrome) (MD-0.18 ml/min; 95% CI, −0.24 to −0.13; chi2-P-value <0.00001). Heterogeneity was 97% and there was publication bias (funnel plot). The level of evidence was mostly3 or 4. The quality of evidence was met (97% of items valued). Conclusion: For the first time, the unstimulated whole salivary flow rate is found to be lower in patients with Sjögren's syndrome compared to controls (healthy and non-SS sicca) through a meta-analysis. (AU)

Humans , Salivary Glands/metabolism , Xerostomia/metabolism , Sjogren's Syndrome/physiopathology , Autoimmunity
J. appl. oral sci ; 29: e20201080, 2021. tab, graf
Article in English | LILACS | ID: biblio-1340115


Abstract Acute and chronic stresses affect the salivary glands, representing the source of plasma BDNF during stressful conditions. Pumpkin is a medicinal plant with an evident antioxidant, anti-inflammatory and potential antidepressant effects. Objective To assess the structural and biochemical effects induced by exposure to chronic unpredictable mild stress (CUMS) on salivary glands of albino rats, and to evaluate the role of pumpkin extract (Pump) in ameliorating this effect. Methodology Four groups (n=10 each) of male albino rats were included in this study: the control, CUMS, Fluoxetine-treated and Pump-treated. The corticosterone, the pro-inflammatory cytokines, tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6), and the oxidant/antioxidant profile were all assessed in the serum. The level of BDNF mRNA was measured in the salivary glands using qRT-PCR. Histopathological changes of the salivary glands were also assessed. Results The depressive-like status was confirmed behaviorally and biochemically. Exposure to CUMS significantly up-regulated (p<0.001) the level of serum corticosterone. CUMS induced degenerative changes in the secretory and ductal elements of the salivary glands evident by increased apoptosis. Both Fluoxetine and Pumpkin significantly up-regulated (p<0.001) BDNF expression in the salivary glands and ameliorated the CUMS-induced histopathological and biochemical alterations in the salivary glands. Pumpkin significantly (p<0.001) increased the serum levels of antioxidant enzymes SOD, GPX and CAT, and reduced the serum levels of the pro-inflammatory cytokines TNF-α, IL-6. Conclusion Pumpkin ameliorates the depressive-like status induced in rats following exposure to chronic stress through exerting a promising anti-inflammatory, antioxidant and anti-depressant-like effects. The pumpkin, subsequently, improved stress-induced structural changes in the salivary glands that might be due to up-regulation of BDNF expression in the glands.

Animals , Rats , Brain , Salivary Glands , Cucurbita
Int. j. odontostomatol. (Print) ; 14(4): 495-500, dic. 2020. tab, graf
Article in Spanish | LILACS | ID: biblio-1134526


RESUMEN: El virus SARS-CoV-2 ingresa al organismo de un individuo susceptible a través de la cavidad oral, nasal o de la mucosa conjuntival; busca ensamblarse por medio de su glicoproteína de superficie o espiga con los receptores de la enzima convertidora de angiotensina 2 que en boca los encontramos con mayor expresión en las células escamosas que recubren el epitelio lingual y las glándulas salivales, una vez que ingresa por medio de la activación de proteasas ingresa a la célula huésped para denudar su RNA viral, a diferencia de otros virus no necesita ir hasta el núcleo de tal forma que en el citoplasma inicia su replicación y utiliza los ribosomas del huésped para formar una gran cantidad de proteínas virales tanto estructurales como accesorias que le permita formar nuevos viriones potencialmente infecciosos; los estomatólogos deben tomar en cuenta esta vía de infección y extremar las medidas para disminuir su carga viral local en la cavidad oral y las barreras físicas de protección para el operador, el paciente y la ergonomía del consultorio.

ABSTRACT: SARS-CoV-2 virus enters the body of a susceptible individual through oral, nasal or conjunctival mucosa, seeking to bind to the spike glycoprotein surface through angiotensin-converting enzyme 2 receptors. These are found in the mouth with a higher expression in oral squamous cells that cover the lingual epithelium and salivary glands. Once proteolytic activation begins, it enters the host cell to denudate its viral RNA. In contrast with other viruses, it does not require nucleus access, and therefore replicates in the cytoplasm using the host's ribosomes to produce great amounts of both structural and accessory viral proteins. Since this generates new and potentially infectious virions, dentists must consider this route of infection and take extreme measures to decrease their viral load in the oral cavity. Physical protection barriers for the operator, the patient and the health and safety of the work place are critical in these cases.

Humans , Pneumonia, Viral , Coronavirus Infections , Pandemics , Betacoronavirus , Salivary Glands/virology , Virology/methods , Peptidyl-Dipeptidase A , Host-Pathogen Interactions/genetics , Mouth
Int. j. morphol ; 38(3): 570-577, June 2020. tab, graf
Article in Spanish | LILACS | ID: biblio-1098289


El ratón (Mus musculus) es un buen modelo animal de experimentación, donde sus glándulas salivales han sido utilizadas en estudios, especialmente de tipo preclínico. Sin embargo, las diferencias en la morfología de estas glándulas y, en particular de las submandibulares, entre humanos y roedores no son de conocimiento común. Por lo anterior, el objetivo de este estudio fue conocer las características morfocuantitativos de la glándula submandibular del ratón macho C57BL/6. Se utilizaron cinco ratones macho, cepa C57BL/ 6, de 11 a 13 semanas de nacidos, con un peso promedio de 30 g. Luego de su ambientación, los ratones fueron eutanasiados. Para el análisis histológico, las glándulas fueron extraídas y fijadas en formalina tamponada por 48 horas, procesada para su inclusión en paraplast y teñidas con hematoxilina y eosina, azul alcián pH 2,5, ácido peryódico de Schiff y Picrosirius Red. Se determinaron las características estereológicas de densidad de longitud (LVcond), de volumen (VVcond) y de superficie (SVcond) de los conductos. En las células acinares se evaluó la densidad de número (NVcel) y la densidad de volumen (VVcel). Para cada variable se obtuvo la media ± desviación estándar. La glándula submandibular se encontraba organizada de manera lobulada, cuyo parénquima era de tipo túbuloacinar mixto con predominio seroso, que se conectaba a través de conductos. Las células acinares presentaban reacción positiva para azul alcián y ácido peryódico de Schiff. El estroma de la glándula contenía principalmente colágeno tipo 1. Los resultados de las mediciones estereológicas fueron: LVcond = 220,02 ± 50,51 mm/mm3; VVcond = 50,48 ± 9,55 %; SVcond = 26,39 ± 5,45 mm2/mm3; NVcel = 16,85 x 104 ± 9,13 × 104/mm3; VVcel = 24,98 ± 6,22 %. Con los análisis presentados es posible realizar investigaciones experimentales, considerando las similitudes y diferencias morfocuantitativas que presenta la glándula submandibular de ratón macho, cepa C57BL/6 con la del humano.

The mouse (Mus musculus) is a good experimental animal model, where murine salivary glands have been widely used in research by their similarities with human glands. Stereology is a tool that has to demonstrated to be valuable in biomedical researc h. Therefore, the objective was to realize an analogy between morphology, function and morpho-quantitative characteristics of the C57BL/6 mouse submandibular gland. Five male mice, C57BL/6, from 11 to 13 weeks old were used, with an average weight of 30 g. After seven days, the mice were euthanized and the samples were extracted. Then, these were processed following the conventional histological processing protocol and stained with H&E, Picrosirius Red, AA and PAS. Finally, the histological and stereological analysis of the samples was performed. In the glandular ducts the length density (LVcond), volume density (VVcond) and surface density (SVcond) were determined; whereas, in the acinar cells the density of number (NVcel) and the density of volume (VVcel) were evaluated. For each stereological variable the mean ± standard deviation was obtained. The histological study revealed no discrepancies as previously described. Descriptive statistical analysis revealed the following: LVcond = 220.02 ± 50.51 mm/mm3; VVcond = 50.48 ± 9.55 %; SVcond = 26.39 ± 5.45 mm2/mm3; NVcel = 168467.38 ± 91288.67 /mm3 and VVcel = 24.98 ± 6.22 %. From this study, it will be possible to conduct comparative experimental research analyzing quantitative results from those presented in this research. From this study, it will be possible to carry out comparative experimental research, analyzing quantitative results from those presented in this investigation.

Animals , Male , Mice , Submandibular Gland/anatomy & histology , Mice, Inbred C57BL/anatomy & histology , Salivary Glands/anatomy & histology , Models, Animal
Int. j. morphol ; 38(3): 755-760, June 2020. tab, graf
Article in English | LILACS | ID: biblio-1098316


SUMMARY: The objective of this study was to describe the effects of monosodium glutamate on the collagen of the parotid gland in an obesity model. 18 newborn male Sprague Dawley rats were used (first control group; second group of MSG1: 4 mg/g of monosodium glutamate weight, 5 doses, and third group of MSG2: 4 mg/g of monosodium glutamate, 5 doses, maintained for 8 and 16 weeks respectively). The content and type of collagen were analyzed, in addition to the levels of cholesterol, glucose, triglycerides and uric acid. Monosodium glutamate produced an increase in the obesity rates of the MSG2 group, in addition to an increase in blood cholesterol, glucose and uric acid levels compared to the control group. Type III collagen in the MSG2 group showed a statistically significant increase. Monosodium glutamate induced obesity, in addition to an increase in type III collagen fibers.

RESUMEN: El objetivo de este estudio fue describir los efectos del glutamato monosódico sobre el colágeno de la glándula parótida en un modelo de obesidad. Se utilizaron 18 ratas Sprague Dawley machos recién nacidas (primer grupo control; segundo grupo MSG1: 4 mg/g de peso de glutamato monosódico, 5 dosis, y tercer grupo MSG2: 4 mg/g de glutamato monosódico, 5 dosis, mantenidas durante 8 y 16 semanas respectivamente). Se analizó el contenido y el tipo de colágeno, además de los niveles de colesterol, glucosa, triglicéridos y ácido úrico. El glutamato monosódico produjo un aumento en las tasas de obesidad del grupo MSG2, además de un aumento en los niveles de colesterol en sangre, glucosa y ácido úrico en comparación con el grupo control. El colágeno tipo III en el grupo MSG2 mostró un aumento estadísticamente significativo. La obesidad inducida por glutamato monosódico, además de un aumento en las fibras de colágeno tipo III.

Animals , Male , Rats , Parotid Gland , Sodium Glutamate/toxicity , Collagen/drug effects , Obesity/chemically induced , Salivary Glands/drug effects , Triglycerides/blood , Uric Acid/blood , Blood Glucose/analysis , Body Weight/drug effects , Cholesterol/blood , Collagen/analysis , Rats, Sprague-Dawley , Disease Models, Animal , Animals, Newborn
Rev. cub. inf. cienc. salud ; 31(2): e1525, abr.-jun. 2020. tab, fig
Article in Spanish | LILACS, CUMED | ID: biblio-1138854


El objetivo del presente trabajo fue analizar el panorama científico internacional relacionado con la investigación de la saliva entre los años 2000 y 2018. Se realizó una investigación observacional, descriptiva, retrospectiva, con descripción cuantitativa de los datos, a través del análisis bibliométrico de documentos. PubMed y Dentistry & Oral Sciences Source han sido seleccionadas como importantes bases de datos bibliométricas en línea para buscar y recuperar documentos. Todos los datos presentados en este estudio se analizaron utilizando los siguientes programas de software: VOSviewer, Publish or Perish, Mendeley Desktop y SPSS versión 19 para Windows. Se utilizaron métodos estadísticos descriptivos e inferenciales. En ambas bases de datos se observaron diferencias en la cantidad de documentos entre los períodos 2000-2009 y 2010-2018 (p < 0,05). En PubMed se registraron 38 969 documentos. Granger, de EE.UU., fue el autor más productivo (0,45 por ciento). Los temas más discutidos estuvieron relacionados con el metabolismo (44,70 por ciento) y la química (37,71 por ciento), en correlación con el estrés (9,51 por ciento), las glándulas salivales (4,65 por ciento) y el cáncer (3,50 por ciento). En Dentistry & Oral Sciences Source se registraron 6 621 documentos. Attin, Suizo-Alemán, fue el autor con más trabajos (0,86 por ciento). La caries dental (11,20 por ciento), el análisis de la saliva (9,41 por ciento) y la enfermedad períodontal (5,89 por ciento) fueron los temas de diagnóstico más estudiados. Durante el período de estudio se incrementó la investigación internacional vinculada con la saliva. Sin embargo, el alcance de los estudios multicéntricos fue menor de lo esperado, dada la enorme carga de los problemas de salud mundiales relacionados con varios componentes químicos de la saliva(AU)

The purpose of the study was to analyze the international scientific panorama concerning research about saliva between the years 2000 and 2018. An observational descriptive retrospective quantitative study was conducted of the data based on bibliometric document analysis. PubMed and Dentistry & Oral Sciences Source were selected as important online bibliometric databases to search for and retrieve documents. All the data presented in the study were analyzed with the following software: VOSviewer, Publish or Perish, Mendeley Desktop and SPSS version 19 for Windows. Descriptive and inferential statistical methods were used. The two databases exhibited differences in the number of documents between the periods 2000-2009 and 2010-2018 (p < 0.05). A total 38 969 documents were recorded in PubMed. Granger, from the USA, was the most productive author (0.45 percent). The topics most commonly discussed had to do with metabolism (44.70 percent) and chemistry (37.71 percent) in relation to stress (9.51 percent), salivary glands (4.65 percent) and cancer (3.50 percent). A total 6 621 documents were recorded in Dentistry & Oral Sciences Source. Attin, a Swiss-German, was the most productive author (0.86 percent). Dental caries (11.20 percent), saliva analysis (9.41 percent) and periodontal disease (5.89 percent) were the diagnostic topics most commonly dealt with. During the study period an increase occurred in international research about saliva. However, the scope of multicenter studies was lesser than expected, given the huge load of world health problems related to several chemical components of saliva(AU)

Humans , Periodontal Diseases , Saliva , Salivary Glands , Dental Caries
Medisan ; 24(1)ene.-feb. 2020. tab
Article in Spanish | LILACS-Express | LILACS, CUMED | ID: biblio-1091165


Introducción: Las inflamaciones agudas y crónicas pueden afectar las glándulas salivales mayores. Objetivo: Evaluar la evolución de los pacientes con procesos inflamatorios de las glándulas salivales mayores tratados con succión desembrozante. Métodos: Se realizó un estudio descriptivo de 49 pacientes con procesos inflamatorios de las glándulas salivales mayores, atendidos en la consulta de Cirugía Maxilofacial del Hospital General Docente Dr. Juan Bruno Zayas Alfonso de Santiago de Cuba, desde septiembre del 2012 hasta junio del 2014. Se utilizó la aspiración quirúrgica para succionar los conductos de las glándulas salivales afectadas. Se estudiaron variables, tales como edad, sexo, glándula salival afectada, agente causal, sintomatología, sesiones de tratamiento y evolución ultrasonográfica. Resultados: Predominaron el grupo etario de 65 años y más (36,7 %) y el sexo femenino (59,2 %). La litiasis fue el factor causal de mayor incidencia y la glándula parótida la más afectada. Los pacientes evolucionaron satisfactoriamente luego de la segunda succión, lo cual demostró la existencia de cambios ultrasonográficos favorables. Conclusiones: La succión desembrozante es un procedimiento sencillo e inocuo que evita el proceder quirúrgico.

Introduction: The acute and chronic inflammations can affect the major salivary glands. Objective: To evaluate the clinical course of patients with inflammatory processes of the major salivary glands treated with depleting suction. Methods: A descriptive study of 49 patients with inflammatory processes of the mayor salivary glands was carried out, they were assisted in the Maxillofacial Surgery service of Dr. Juan Bruno Zayas Alfonso Teaching General Hospital in Santiago de Cuba, from September, 2012 to June, 2014. The surgical aspiration was used to suck the tubes of the affected salivary glands. Some variables were studied, such as age, sex, affected salivary gland, causal agent, symptomatology, treatment sessions and ultrasonographic course. Results: There was a prevalence of the 65 and over age group (36.7 %) and the female sex (59.2 %). Lithiasis was the causal factor of more incidence and the parotid gland the most affected one. Patients had a favorable clinical course after the second suction, which demonstrated the existence of favorable ultrasonographic changes. Conclusions: Depleting suction is a simple and innocuous procedure that avoids operation.

Salivary Gland Diseases , Salivary Glands , Suction , Salivary Gland Calculi
J. Health Biol. Sci. (Online) ; 8(1): 1-6, 20200101. ilus
Article in English | LILACS | ID: biblio-1129911


Objective: To evaluate the radioprotective effect of a homeopathic solution in salivary function and parotid glands morphology of irradiated rats. Materials and Methods: The sample consisted of 150 rats randomly divided into 6 groups. The groups were named based on the substance administered: Control- saline solution; Irradiated Control- saline solution and 15 Gy of X radiation; Alcohol-hydroalcoholic solution dynamized at 15 CH; Irradiated Alcohol- hydroalcoholic solution dynamized at 15 CH and 15 Gy of X radiation; Homeopathy- 0.25 ml (1mL/kg) of the irradiated hydroalcoholic solution and dynamized at 15 CH; Irradiated homeopathy- homeopathic solution and 15 Gy of X radiation. Each group was subdivided into 5 different subgroups, based on the time point of euthanasia: 12 hours, 3, 10, 17, and 24 days. The medication was administered for 7 days before and 7 days after the radiation treatment. On the day of euthanasia, salivation was induced with pilocarpine and collected. The animals were then sacrificed and the parotid glands were removed. Results: Salivary function analysis showed that only group irradiated homeopathy euthanized on day 17 had a statistically significant difference when compared to other irradiated groups, presenting a higher salivation flow rate. The only group that showed a statistically significant difference in the number of acini over time was the irradiated alcohol group, which presented a tendency of reduction. Conclusion: The homeopathic solution presented a late radioprotective effect based on salivary function and morphological analysis of the parotid gland.

Objetivo: Avaliar o efeito radioprotetor de uma solução homeopática na função salivar e na morfologia das glândulas parótidas de ratos irradiados. Materiais e Métodos: A amostra foi composta por 150 ratos divididos aleatoriamente em 6 grupos. Os grupos foram nomeados com base na substância administrada: solução salina controle; Solução salina controle irradiada e 15 Gy de radiação X; Solução álcool-hidroalcoólica dinamizada a 15 CH; Solução hidroalcoólica de álcool irradiado dinamizada a 15 CH e 15 Gy de radiação X; Homeopatia - 0,25 ml (1mL / kg) da solução hidroalcoólica irradiada e dinamizada a 15 CH; Homeopatia irradiada - solução homeopática e 15 Gy de radiação X. Cada grupo foi subdividido em 5 subgrupos diferentes, com base no tempo da eutanásia: 12 horas, 3, 10, 17 e 24 dias. O medicamento foi administrado por 7 dias antes e 7 dias após o tratamento com radiação. No dia da eutanásia, a salivação foi induzida com pilocarpina e coletada. Os animais foram então sacrificados e as glândulas parótidas foram removidas. Resultados: A análise da função salivar mostrou que apenas a homeopatia irradiada por grupo sacrificada no dia 17 apresentou diferença estatisticamente significante quando comparada a outros grupos irradiados, apresentando maior taxa de fluxo de salivação. O único grupo que apresentou diferença estatisticamente significante no número de ácinos ao longo do tempo foi o grupo álcool irradiado, o qual apontou uma tendência de redução. Conclusão: A solução homeopática apresentou efeito radioprotetor tardio baseado na função salivar e na análise morfológica da glândula parótida.

Parotid Gland , Homeopathy , Radiotherapy , Salivary Glands , Salivation , Therapeutics , X-Rays
Article in English | LILACS, BBO | ID: biblio-1135579


Abstract The world is under the threat of the novel coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Despite several efforts to contain the disease spread, it still constitutes a public health emergency of international concern. Several published reports in the scientific literature called attention of the oral cavity as the potential route of infection, the implications for dental practice and the use of saliva in the diagnose of the COVID-19. The aim of this article is to provide an overview of the literature on the salivary glands and saliva in the context of SARS-CoV-2 infection. A brief discussion of taste disturbances and oral findings in COVID-19 patients is also presented. The literature shows that SARS-CoV-2 could infect the salivary glands. It is not possible, however, to make speculations regarding them as reservoirs for the SARS-CoV-2. In addition, patients with COVID-19 presented several oral repercussions, including hyposalivation and taste disturbances. A few reports showed oral ulcers and blisters associated with SARS-CoV-2 infection. However, it remains not fully understood and might lead to erroneous assumptions. Overall, further studies are necessary to understand the real role of salivary glands and saliva in the context of SARS-CoV-2 infection.

Saliva , Salivary Glands , Public Health , Coronavirus , Severe Acute Respiratory Syndrome/pathology , Xerostomia , Brazil/epidemiology , Oral Ulcer
São Paulo; s.n; 20200000. 73 p.
Thesis in Portuguese | LILACS, BBO | ID: biblio-1119549


Na classificação da Organização Mundial de Saúde (OMS) de 2017 foram relatados 11 tipos de neoplasias benignas e 20 tipos de neoplasias malignas das glândulas salivares. Dentre estes últimos está o carcinoma de células claras de glândula salivar, uma neoplasia rara que corresponde a menos de 1% dos carcinomas de glândulas salivares. Tem no seu aspecto histológico semelhança com outras neoplasias que também apresentam células claras, tendo a imuno-histoquímica e os testes moleculares importante papel para seu diagnóstico. O objetivo do presente trabalho foi avaliar os aspectos histopatológicos, imuno-histoquímicos e biomoleculares, correlacionando com aspectos clínicos, dos casos carcinomas de células claras de glândula salivar, diagnosticados no serviço de Patologia Oral e Maxilofacial da Faculdade de Odontologia da Universidade de São Paulo (USP) diagnosticados no período entre 1997 e 2018. Em todos os casos foram avaliados os cortes histológicos, reações de imuno-histoquímica para citoqueratinas 7 e 14 (CK7 e CK14), proteína p63, proteína S-100 e actina de musculo liso (SMA), além de teste molecular da reação em cadeia da polimerase (PCR) em tempo real para pesquisa da expressão do gene de fusão EWSR1-ATF1. No levantamento dos casos do serviço de Patologia Oral e Maxilofacial da Faculdade de odontologia da USP no período de 1997 até 2018 observamos 11 lesões com as características histológicas e imuno-histoquímicas para se caracterizar como carcinoma de células claras de glândulas salivares, sendo 81,81% em mulheres, 77,77% de cor da pele branca, média de idade 56,42 anos e as regiões mais acometidas foram o palato e a mucosa jugal com 36,36% dos casos cada. Histologicamente nove casos apresentavam hialinização; e invasão neural e necrose estavam presentes em 6 e 2 dos casos respectivamente. Todos os casos foram positivos para ácido periódico-Schiff (PAS), e na imuno-histoquímica CK7 e CK14 estavam presentes em todos os casos, assim como p63. Já S-100 e SMA foram negativos em todos. No teste molecular de PCR apenas duas lesões mostraram o gene de fusão EWSR1-ATF1. Concluímos que o carcinoma de células claras é mais prevalente em mulheres com idade entre 50 e 60 anos, com predileção pelo palato. A histologia associada à imuno-histoquímica muitas vezes é suficiente para se fechar esse diagnóstico, por se tratar de uma lesão com um só tipo celular. A hialinização nem sempre está presente na lesão e a invasão neural é muito comum. Os testes moleculares devem ser utilizados como auxilio no diagnóstico, e o resultado da reação em cadeia da polimerase (PCR) para o gene EWSR1-ATF1 é fiel apenas em lesões recentes.

Salivary Glands , Carcinoma , Adenocarcinoma, Clear Cell
São Paulo; s.n; 20200000. 158 p.
Thesis in Portuguese | LILACS, BBO | ID: biblio-1119592


As neoplasias de glândulas salivares são pouco frequentes e, em apenas 9 a 23% dos casos, as glândulas menores são acometidas. É muito comum, em estudos retrospectivos, o agrupamento das neoplasias de glândulas salivares maiores e menores, dificultando a identificação da real incidência das últimas. Realizamos um levantamento das neoplasias de glândulas salivares menores nos últimos 21 anos para identificar o perfil dos pacientes acometidos. Durante o período foram diagnosticados 72.286 casos no serviço, desses 687 (0,95%) eram neoplasias de glândulas salivares menores: 345 malignas (50,2%) e 342 benignas (49,8%). A região mais acometida foi o palato (53%). Os pacientes foram na sua maioria mulheres (65,5%) entre 5ª e 6ª década de vida (16,5% cada), a idade média dos pacientes foi de 48,44 anos variando de 8 a 93 anos. Os diagnósticos mais comuns foram: Adenoma pleomórfico (36,4%) e Carcinoma mucoepidermoide (16,9%). Nossos resultados mostram que, 41,5% das neoplasias malignas foram submetidas a biópsias excisionais, o que demonstra uma necessidade da melhor caracterização desse tipo de lesão para auxiliar na conduta clínica e no tratamento dos pacientes. Levantamos algumas características que podem indicar maiores chances de um diagnostico histológico maligno ou benigno: entre a quarta e a sexta década não é possível notar uma maior frequência entre neoplasias benignas e malignas; na primeira, sétima, oitava e nona décadas nota-se uma maior acometimento por neoplasias malignas; na segunda, terceira e décima há uma predominância das neoplasias benignas. As pacientes do sexo feminino apresentam uma tendência a neoplasias malignas, enquanto os pacientes do sexo masculino apresentam uma tendência inversa. Algumas regiões são mais propensas às neoplasias malignas: língua, região retromolar, assoalho bucal e mucosa jugal; outras apresentam uma frequência muito próxima: lábio inferior, palato e fundo de sulco; e regiões como lábio e lábio superior tem tendência aà neoplasias benignas. As lesões ulceradas e manchas tem uma maior chance de serem malignas; as bolhas de serem benignas e os nódulos tem chances semelhantes. As lesões enegrecidas foram exclusivamente malignas e há uma tendência maior para o diagnóstico de malignidade em leões brancas, vermelhas e roxas. Nas lesões amarelas há uma distribuição semelhante entre benignas e malignas e as lesões com cor de mucosa normal tendem a ser benignas. Os pacientes tabagistas apresentaram mais neoplasias benignas, os não tabagistas apresentaram relação semelhante entre neoplasias benignas e malignas e os pacientes ex tabagistas tiveram somente neoplasia maligna. Os pacientes etilistas apresentaram mais neoplasias benignas que malignas, enquanto os pacientes não etilistas apresentaram uma frequência bastante similar. As lesões sintomáticas tiveram maior tendência à malignidade. Lesões de até 6 cm tem tendência semelhante a neoplasias benignas e malignas, enquanto as lesões com mais de 7 cm apresentam uma maior tendência à malignidade. As lesões com evolução de até 6 meses, e entre 25 a 36 meses, tem mais tendência à malignidade; há uma similaridade muito grande entre os diagnósticos benignos e malignos de 7 a 24 meses, enquanto, as lesões com mais de 36 meses têm menor tendência a malignidade.

Salivary Glands , Salivary Glands, Minor , Salivary Gland Neoplasms
Niger. J. Dent. Res ; 5(2): 131-135, 2020. ilus
Article in English | AIM | ID: biblio-1266997


Objective: To determine relative saliva viscosity (RSV) among carious and non-carious young adult Nigerians. Methods: Participants included in this study were young adults of both sexes aged 11-40 years recruited and categorized as non-carious individuals as those with Decayed, Missing and Filled Teeth (DMFT) of 0 and carious individual as those with DMFT of ≥1. The collection of unstimulated saliva samples was done using disposable cups and aspirated into 10 mls syringe. RSV was determined by allowing saliva to flow through a 10 mls syringe and measuring the rate of flow from 2 mls to 0 mls. The timing was done by means of a stopwatch and recorded in seconds. The plunger of the disposable syringe is carefully removed to allow the saliva to flow through the adaptor without the needle. The start off for the flow is usually above 2 mls but timing start off timing is at 2 mls as a precautionary measure. This is a simple and accurate model for measuring the viscousity of liquid (Figure 1). Statistical tests used in this study were independent t-test and one-way ANOVA. Statistical significance was at P <0.05. Results: The participants in this study were between 17 and 40 years with a mean age of 27.81±6.56 years. The overall mean relative saliva viscosity among the participants was 1.93±0.26. The mean relative saliva viscosity was 2.39±0.65 among participants aged 31-40years old. Female participants had mean relative saliva viscosity of 2.09±0.40. Participants with dental caries had mean relative saliva viscosity of 2.41±0.44. Conclusion: Relative saliva viscosity measured with a 10 disposable syringe was found to be higher in participants with dental caries than those without. Strategies to prevent dental caries should include efforts to increase the viscosity of saliva. Salivary viscosity of 1.16-1.66 P oise (P) (which is the CGS unit of viscosity) signifies oral cavity without dental caries. The SI Unit of viscosity is pascal second (Pa s). Ten poise equal one pascal second making the centipoise (cP) and millipascal second (mPa s) identical. Further studies on salivary viscosity and severity of dental caries is recommended

Dental Caries , Nigeria , Salivary Glands , Viscosity , Young Adult
J. venom. anim. toxins incl. trop. dis ; 26: e20190095, 2020. tab, graf, ilus
Article in English | ID: biblio-1135162


Conopeptides are neuropharmacological peptides derived from the venomous salivary glands of cone snails. Among 29 superfamilies based on conserved signal sequences, T-superfamily conotoxins, which belong to the smallest group, include four different frameworks that contain four cysteines denominated I, V, X and XVI. In this work, the primary structure and the cysteine connectivity of novel conotoxin of Conus bandanus were determined by tandem mass spectrometry using collision-induced dissociation. Methods: The venom glands of C. bandanus snails were dissected, pooled, and extracted with 0.1% trifluoroacetic acid in three steps and lyophilized. The venom was fractionated and purified in an HPLC system with an analytical reversed-phase C18 column. The primary peptide structure was analyzed by MALDI TOF MS/MS using collision-induced dissociation and confirmed by Edman's degradation. The peptide's cysteine connectivity was determined by rapid partial reduction-alkylation technique. Results: The novel conotoxin, NGC1C2(I/L)VREC3C4, was firstly derived from de novo sequencing by MS/MS. The presence of isoleucine residues in this conotoxin was confirmed by the Edman degradation method. The conotoxin, denominated Bn5a, belongs to the T1-subfamily of conotoxins. However, the disulfide bonds (C1-C4/C2-C3) of Bn5a were not the same as found in other T1-subfamily conopeptides but shared common connectivities with T2-subfamily conotoxins. The T1-conotoxin of C. bandanus proved the complexity of the disulfide bond pattern of conopeptides. The homological analysis revealed that the novel conotoxin could serve as a valuable probe compound for the human-nervous-system norepinephrine transporter. Conclusion: We identified the first T1-conotoxin, denominated Bn5a, isolated from C. bandanus venom. However, Bn5a conotoxin exhibited unique C1-C4/C2-C3 disulfide connectivity, unlike other T1-conotoxins (C1-C3/C2-C4). The structural and homological analyses herein have evidenced novel conotoxin Bn5a that may require further investigation.(AU)

Animals , Peptides , Conotoxins , Disulfides , Conus Snail , Salivary Glands
Article in Korean | WPRIM | ID: wpr-785347


Immunoglobulin (Ig) G4-related disease (IgG4-RD) is newly recognized immune-mediated and fibroinflammatory conditions with various organ involvements. Any organs can be involved, but the pancreas, salivary gland, lymph nodes, and orbit are known to be commonly involved organs. A 54-year-old man presented with complaint of psoriasis like skin rash developed 4 years prior to admission. Although he had been treated for skin rash, the extent of skin lesions increased as well as hypereosinophilia, and multiple lymphadenopathies were newly developed. The patient was diagnosed with IgG4-RD by serum IgG4 levels and histologic examination of the inguinal lymph node. One month after treatment with steroid and azathioprine, his skin rash and lymphadenopathies resolved with improvement and eosinophil count was within the normal range. We herein report a case of a IgG4-RD patient associated with psoriasis-like skin rash and hypereosinophilic syndrome.

Azathioprine , Eosinophils , Exanthema , Humans , Hypereosinophilic Syndrome , Immunoglobulin G , Immunoglobulins , Lymph Nodes , Middle Aged , Orbit , Pancreas , Psoriasis , Reference Values , Salivary Glands , Skin
Int. j. morphol ; 37(4): 1564-1571, Dec. 2019. tab
Article in Spanish | LILACS | ID: biblio-1040170


Las glándulas salivales humanas pueden ser gravemente lesionadas por la radioterapia utilizada contra neoplasias de cabeza y cuello, produciendo hiposialia y xerostomía, las cuales afectan la salud oral y sistémica, mermando la calidad de vida de la persona. Los tratamientos convencionales actuales están diseñados para disminuir los síntomas, sin actuar sobre los cambios fisiopatológicos que se dan a nivel glandular. Esta revisión intenta analizar aquellas terapias preventivas y/o curativas que están desarrollándose en el campo biomolecular y que tienen un futuro prometedor por sus características innovadoras: terapia génica, terapia con células madre y terapia con factores de crecimiento. Se evidencia un aporte adicional de la nanotecnología, la cual está mejorando las vías de aplicación de los tratamientos.

Human salivary glands can be seriously injured by the radiotherapy used against head and neck neoplasms, producing hyposialia and xerostomy, which affect oral and systemic health, diminishing the person's quality of life. Current conventional treatments are designed to reduce symptoms, without acting on the pathophysiological changes that occur at the glandular level. This review attempts to analyze those preventive and /or curative therapies that are developing in the biomolecular field and that have a promising future due to their innovative features: Gene therapy, stem cell therapy and growth factor therapy. An additional contribution of nanotechnology is evident, which is improving the routes of treatment application.

Humans , Radiotherapy/adverse effects , Salivary Gland Diseases/prevention & control , Stem Cells/physiology , Genetic Therapy/methods , Intercellular Signaling Peptides and Proteins/therapeutic use , Radiation Injuries/prevention & control , Radiation-Protective Agents/therapeutic use , Salivary Gland Diseases/therapy , Salivary Glands/radiation effects , Xerostomia/prevention & control , Nanotechnology