Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 51
Filter
1.
Braz. j. med. biol. res ; 54(7): e10240, 2021. tab, graf
Article in English | LILACS | ID: biblio-1249316

ABSTRACT

Dengue is the most important arthropod-borne viral disease worldwide. Infection with any of the four dengue virus (DENV) serotypes can be asymptomatic or lead to disease with clinical symptoms ranging from undifferentiated and self-limiting fever to severe dengue disease, which can be fatal in some cases. Currently, no specific antiviral compound is available for treating DENV. The aim of this study was to identify compounds in plants from Paraguayan folk medicine with inhibitory effects against DENV. We found high virucidal activity (50% maximal effective concentration (EC50) value of 24.97 µg/mL) against DENV-2 in the ethanolic extract of the roots of Solanum sisymbriifolium Lam. (Solanaceae) without an evident cytotoxic effect on Vero E6 cells. Three saponins isolated from the root extract showed virucidal effects (EC50 values ranging from 24.9 to 35.1 µg/mL) against DENV-2. Additionally, the saponins showed inhibitory activity against yellow fever virus (EC50 values ranging from 126 to 302.6 µg/mL), the prototype virus of the Flavivirus genus, suggesting that they may also be effective against other members of this genus. Consequently, these saponins may be lead compounds for the development of antiviral agents.


Subject(s)
Saponins/pharmacology , Solanum , Dengue Virus , Antiviral Agents/pharmacology , Virus Replication , Yellow fever virus
2.
Article in Chinese | WPRIM | ID: wpr-888171

ABSTRACT

Astragali Radix is a traditional Chinese herbal medicine with a long history, which has the functions of tonifying Qi and promoting urination and granulation. Astragalosides are the main effective components of Astragali Radix, and more than 40 triterpenoid saponins have been obtained from Astragalus membranaceus and its related plants, mainly including astragalosides Ⅰ-Ⅷ, isoastragalosides Ⅰ, Ⅱ, and Ⅳ, acetylastragalosides, and soyasaponins. Astragalosides have a wide range of biological activities, such as immunomodulation, antioxidation, and neuroprotection. Nervous system diseases seriously affect people's quality of life, threaten human physical and mental health, and impose a burden on families and society. As natural drugs, astragalosides have good preventive and therapeutic effects on central nervous system diseases. This paper reviews the main pharmacological effects and mechanisms of astragalosides in the treatment of multiple sclerosis, Parkinson's disease, Alzheimer's disease, and cerebral ischemic stroke and proposes the research prospects and potential problems, aiming to provide reference for the clinical application and basic research of astragalosides.


Subject(s)
Astragalus Plant , Astragalus propinquus , Drugs, Chinese Herbal , Humans , Nervous System Diseases , Quality of Life , Saponins/pharmacology
3.
Article in Chinese | WPRIM | ID: wpr-888020

ABSTRACT

To explore the effect of ophiopogonin D on main fatty acid metabolic enzymes in human cardiomyocyte AC-16,so as to provide reference for cardiovascular protection mechanism and safe clinical application of Ophiopogon japonicus.CCK-8 (cell counting kit-8) was used to detect the effect of different concentrations of ophiopogonin D on the viability of cardiomyocytes.Meanwhile,the effect of different concentrations of ophiopogonin D on the morphology and quantity of cardiomyocytes was observed under microscope.The effect of ophiopogonin D on the mRNA expression of CYP2J2,CYP4F3,CYP4A11,CYP4A22 and CYP4F2 in cardiomyocytes was detected by RT-PCR.Western blot was used to detect the protein expression of CYP4F3 in different concentrations of ophiopogonin D.Compared with the control group,low-concentration ophiopogonin D had no effect on the viability of cardiomyocytes.However,ophiopogonin D with a concentration of higher than 20μmol·L~(-1)could promote the viability.Under the microscope,ophiopogonin D with a concentration of below 100μmol·L~(-1)had no significant effect on the morphology and number of cardiomyocytes.RT-PCR results showed that compared with the control group,5μmol·L~(-1)ophiopogonin D could slightly up-regulate mRNA expressions of CYP2J2 and CYP4F3,while high-concentration ophiopogonin D (10 and 20μmol·L~(-1)) could significantly induce mRNA expressions of CYP2J2and CYP4F3 in a dose-dependent manner (P<0.05).The same concentration of ophiopogonin D had a little effect on the mRNA expressions of CYP4A11,CYP4A22 and CYP4F2.Western blot results showed that 20μmol·L~(-1)ophiopogonin D could significantly induce the protein expression of CYP4F3 in a dose-dependent manner (P<0.05).Based on the above results,ophiopogonin D (less than100μmol·L~(-1)) has no effect on the viability of AC-16 cardiomyocytes.Ophiopogonin D (less than 100μmol·L~(-1)) can selectively induce the expressions of CYP2J2 and CYP4F3,regulate the metabolic pathway of fatty acid signaling molecules,and thus protecting the cardiovascular system.


Subject(s)
Fatty Acids , Humans , Myocytes, Cardiac , Saponins/pharmacology , Spirostans/pharmacology
4.
Article in English | WPRIM | ID: wpr-922104

ABSTRACT

OBJECTIVE@#To elucidate the underlying mechanism of Panax notoginseng saponin (PNS) on gastric epithelial cell injury and barrier dysfunction induced by dual antiplatelet (DA).@*METHODS@#Human gastric mucosal epithelial cell (GES-1) was cultured and divided into 4 groups: a control, a DA, a PNS+DA and a LY294002+PNS+DA group. GES-1 apoptosis was detected by flow cytometry, cell permeability were detected using Transwell, level of prostaglandins E2 (PGE2), 6-keto-prostaglandin F1α (6-keto-PGF1α) and vascular endothelial growth factor (VEGF) in supernatant were measured by enzyme linked immunosorbent assay (ELISA), expression of phosphatidylinositide 3-kinase (PI3K), phosphorylated-PI3K (p-PI3K), Akt, phosphorylated-Akt (p-Akt), cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), glycogen synthase kinase-3β (GSK-3β) and Ras homolog gene family member A (RhoA) were measured by Western-blot.@*RESULTS@#DA induced apoptosis and hyper-permeability in GES-1, reduced supernatant level of PGE2, 6-keto-PGF1α and VEGF (P<0.05). Addition of PNS reduced the apoptosis of GES-1 caused by DA, restored the concentration of PGE2, 6-keto-PGF1α and VEGF (P<0.05). In addition, PNS attenuated the alteration of COX-1 and COX-2 expression induced by DA, up-regulated p-PI3K/p-Akt, down-regulated RhoA and GSK-3β. LY294002 mitigated the effects of PNS on cell apoptosis, cell permeability, VEGF concentration, and expression of RhoA and GSK-3β significantly.@*CONCLUSIONS@#PNS attenuates the suppression on COX/PG pathway from DA, alleviates DA-induced GES-1 apoptosis and barrier dysfunction through PI3K/Akt/ VEGF-GSK-3β-RhoA network pathway.


Subject(s)
Cyclooxygenase 1 , Epithelial Cells/metabolism , Glycogen Synthase Kinase 3 beta , Humans , Panax notoginseng , Phosphatidylinositol 3-Kinases/metabolism , Platelet Aggregation Inhibitors , Proto-Oncogene Proteins c-akt/metabolism , Saponins/pharmacology , Vascular Endothelial Growth Factor A , rhoA GTP-Binding Protein
5.
Article in Chinese | WPRIM | ID: wpr-921645

ABSTRACT

The present study investigated the effects of chikusetsu saponin Ⅳa(CHS Ⅳa) on isoproterenol(ISO)-induced myocardial hypertrophy in rats and explored the underlying molecular mechanism. ISO was applied to establish a rat model of myocardial hypertrophy, and CHS Ⅳa(5 and 15 mg·kg~(-1)·d~(-1)) was used for intervention. The tail artery blood pressure was measured. Cardiac ultrasound examination was performed. The ratio of heart weight to body weight(HW/BW) was calculated. Morphological changes in the myocardial tissue were observed by HE staining. Collagen deposition in the myocardial tissue was observed by Masson staining. The mRNA expression of myocardial hypertrophy indicators(ANP and BNP), autophagy-related genes(Atg5, P62 and beclin1), and miR199 a-5 p was detected by qRT-PCR. Atg5 protein expression was detected by Western blot. The results showed that the model group exhibited increased tail artery blood pressure and HW/BW ratio, thickened left ventricular myocardium, enlarged myocardial cells, disordered myocardial fibers with widened interstitium, and a large amount of collagen aggregating around the extracellular matrix and blood vessels. ANP and BNP were largely expressed. Moreover, P62 expression was up-regulated, while beclin1 expression was down-regulated. After intervention by CHS Ⅳa at different doses, myocardial hypertrophy was ameliorated and autophagy activity in the myocardial tissue was enhanced. Meanwhile, miR199 a-5 p expression declined and Atg5 expression increased. As predicted by bioinformatics, Atg5 was a target gene of miR199 a-5 p. CHS Ⅳa was capable of preventing myocardial hypertrophy by regulating autophagy of myocardial cells through the miR-199 a-5 p/Atg5 signaling pathway.


Subject(s)
Animals , Cardiomegaly/genetics , Isoproterenol , Myocardium , Myocytes, Cardiac , Oleanolic Acid/analogs & derivatives , Rats , Saponins/pharmacology
6.
Article in Chinese | WPRIM | ID: wpr-879186

ABSTRACT

Non-alcoholic steatohepatitis(NASH) was induced by high-sugar and high-fat diet in mice to investigate the intervention effect of total saponins from Panax japonicus(TSPJ) and explore its possible mechanism. Mice were fed with high-sugar and high-fat diet to establish NASH model, and intervened with different doses of TSPJ(15, 45 mg·kg~(-1)). The animals were fed for 26 weeks. The histomorphology and pathological changes of liver tissues were observed by HE staining. The transcriptional expression levels of miR-199 a-5 p, autophagy related gene 5(ATG5) and inflammatory cytokines interleukin-6(IL-6), interleukin-1β(IL-1β) and tumor necrosis factor α(TNF-α) in mouse liver were measured by quantitative Real-time polymerase chain reaction(qRT-PCR). Western blot was used to detect the expression of autophagy-related proteins ATG5, P62/SQSTM1(P62), and microtubule-associated protein light chain 3(LC3)-I/Ⅱ proteins in mouse liver. The expression of P62 protein was detected by immunofluorescence staining. In order to verify the targeting regulation relationship between miR-199 a-5 p and ATG5, miR mimic/inhibitor NC and miR-199 a-5 p mimic/inhibitor were transfected into Hepa 1-6 cells, and the expression of ATG5 mRNA and protein was detected. pMIR-reportor ATG5-3'UTR luciferase reporter gene plasmid was constructed and co-transfected with miR mimic/inhibitor NC and miR-199 a-5 p mimic/inhibitor into Hepa 1-6 cells to detect luciferase activity. In vivo, HE staining in the model group showed typical fatty degeneration and inflammatory infiltration, with increased expression of miR-199 a-5 p and decreased expression of ATG5 mRNA and protein. The expression of autophagy-associated protein P62 increased significantly, the ratio of LC3Ⅱ/Ⅰ decreased, and the transcriptional expression of inflammatory factors increased significantly. After the intervention by TSPJ, the pathological performance of liver tissue was significantly improved, the expression of miR-199 a-5 p decreased and the expression of ATG5 mRNA and protein increased, the expression of autophagy-associated protein P62 decreased significantly, the ratio of LC3Ⅱ/Ⅰ increased, and the transcriptional expression of inflammatory cytokines IL-6, IL-1β and TNF-α decreased significantly. In vitro, it was found that the expression of ATG5 mRNA and protein and luciferase activity decreased significantly in miR-199 a-5 p overexpression cells, while after inhibition of miR-199 a-5 p expression, the expression level of ATG5 mRNA and protein and luciferase activity increased. The results showed that TSPJ can improve NASH in mice fed with high-sugar and high-fat diet, and its mechanism may be related to the regulation of miR-199 a-5 p/ATG5 signal pathway, the regulation of autophagy activity and the improvement of inflammatory response of NASH.


Subject(s)
Animals , Autophagy , Autophagy-Related Protein 5 , Mice , MicroRNAs/genetics , Non-alcoholic Fatty Liver Disease/genetics , Panax , Saponins/pharmacology
7.
Article in Chinese | WPRIM | ID: wpr-878984

ABSTRACT

One new and two known dammarane-type saponins were isolated from the leaves of Gynostemma pentaphyllum using various chromatographic methods. Their structures were identified by HR-ESI-MS,~( 1)H-NMR, ~(13)C-NMR, 2 D-NMR spectra as 2α,3β,12β,20,24(S)-tetrahdroxydammar-25-en-3-O-[β-D-glucopyranosyl(1→2)-β-D-glucopyranosyl]-20-O-β-D-xylopyranosyl(1→6)-β-D-glucopyranoside(1, a new compound, namely gypenoside J5) and 2α,3β,12β,20,24(R)-tetrahdroxydammar-25-en-3-O-[β-D-glucopyranosyl(1→2)-β-D-glucopyranosyl]-20-O-β-D-xylopyranosyl(1→6)-β-D-glucopyranoside(2) and 2α,3β,12β,20-tetrahydroxy-25-hydroperoxy-dammar-23-en-3-O-[β-D-glucopyranosyl(1→2)][β-D-glucopyranosyl]-20-O-[β-D-xylopyranosyl(1→6)]-β-D-glucopy-ranoside(3), respectively. Compounds 1 and 2 were a pair of C-24 epimers. All compounds showed weak cytotoxicity agxinst H1299, HepG2, PC-3, SH-SY5 Y cancer cell lines. However, they exerted protective effect against SH-SY5 Y cellular damage induced by H_2O_2 dose-dependently, of which compound 1 displayed the strongest antioxidant effect. The present study suggested that G. pentaphyllum has antioxidative potential and the saponins from G. pentaphyllum are considered as the active compounds with neuroprotecitve effect.


Subject(s)
Gynostemma , Molecular Structure , Neuroprotective Agents/pharmacology , Saponins/pharmacology , Triterpenes/pharmacology
8.
Frontiers of Medicine ; (4): 79-90, 2021.
Article in English | WPRIM | ID: wpr-880969

ABSTRACT

Natural killer (NK) cells, a type of cytotoxic lymphocytes, can infiltrate into ischemic brain and exacerbate neuronal cell death. Astragaloside IV (ASIV) is the major bioactive ingredient of Astragalus membranaceus, a Chinese herbal medicine, and possesses potent immunomodulatory and neuroprotective properties. This study investigated the effects of ASIV on post-ischemic brain infiltration and activation of NK cells. ASIV reduced brain infarction and alleviated functional deficits in MCAO rats, and these beneficial effects persisted for at least 7 days. Abundant NK cells infiltrated into the ischemic hemisphere on day 1 after brain ischemia, and this infiltration was suppressed by ASIV. Strikingly, ASIV reversed NK cell deficiency in the spleen and blood after brain ischemia. ASIV inhibited astrocyte-derived CCL2 upregulation and reduced CCR2


Subject(s)
Animals , Brain , Histone Deacetylases , Killer Cells, Natural , Rats , Saponins/pharmacology , Triterpenes/pharmacology
9.
Braz. j. med. biol. res ; 53(1): e9085, Jan. 2020. graf
Article in English | LILACS | ID: biblio-1055483

ABSTRACT

Total Panax notoginseng saponin (TPNS) is the main bioactivity compound derived from the roots and rhizomes of Panax notoginseng (Burk.) F.H. Chen. The aim of this study was to investigate the effectiveness of TPNS in treating vascular neointimal hyperplasia in rats and its mechanisms. Male Sprague-Dawley rats were randomly divided into five groups, sham (control), injury, and low, medium, and high dose TPNS (5, 10, and 20 mg/kg). An in vivo 2F Fogarty balloon-induced carotid artery injury model was established in rats. TPNS significantly and dose-dependently reduced balloon injury-induced neointimal area (NIA) (P<0.001, for all doses) and NIA/media area (MA) (P<0.030, for all doses) in the carotid artery of rats, and PCNA expression (P<0.001, all). The mRNA expression of smooth muscle (SM) α-actin was significantly increased in all TPNS groups (P<0.005, for all doses) and the protein expression was significantly increased in the medium (P=0.006) and high dose TPNS (P=0.002) groups compared to the injury group. All the TPNS doses significantly decreased the mRNA expression of c-fos (P<0.001). The medium and high dose TPNS groups significantly suppressed the upregulation of pERK1/2 protein in the NIA (P<0.025) and MA (P<0.004). TPNS dose-dependently inhibited balloon injury-induced activation of pERK/p38MAPK signaling in the carotid artery. TPNS could be a promising agent in inhibiting cell proliferation following vascular injuries.


Subject(s)
Animals , Male , Rats , Saponins/pharmacology , Carotid Artery Injuries/prevention & control , p38 Mitogen-Activated Protein Kinases/metabolism , Panax notoginseng/drug effects , Neointima/pathology , Immunohistochemistry , Signal Transduction , Up-Regulation , Rats, Sprague-Dawley , Carotid Artery Injuries/etiology , Real-Time Polymerase Chain Reaction , Hyperplasia
10.
Electron. j. biotechnol ; 43: 41-47, Jan. 2020. graf, ilus
Article in English | LILACS | ID: biblio-1087517

ABSTRACT

Background: The harmful effects of type 2 diabetes mellitus and its complications have become a major global public health problem. In this study, the effects of Momordica charantia saponins (MCS) on lipid metabolism, oxidative stress, and insulin signaling pathway in type 2 diabetic rats were investigated. Results: MCS could attenuate the tendency of weight loss of the model rats. It could also improve glucose tolerance; reduce fasting blood glucose, nonesterified fatty acid, triglyceride, and total cholesterol; and increase the insulin content and insulin sensitivity index of the rats. The activity of superoxide dismutase and catalase increased, and the content of malondialdehyde decreased in the liver and pancreas tissues of rats in MCS-treated groups significantly. In addition, the expression of p-IRS-1 (Y612) and p-Akt (S473) increased, and the expression of p-IRS-1 (S307) decreased in the liver tissues and pancreas tissues of rats in MCS-treated groups significantly. Conclusion: MCS has an antidiabetic effect, which may be related to its improving the lipid metabolism disorder, reducing oxidative stress level, and regulating the insulin signaling pathway.


Subject(s)
Animals , Male , Rats , Saponins/therapeutic use , Momordica charantia/chemistry , Diabetes Mellitus, Experimental/drug therapy , Hypoglycemic Agents/therapeutic use , Pancreas/drug effects , Saponins/pharmacology , Blood Glucose/drug effects , Body Weight , Insulin Resistance , Rats, Wistar , Oxidative Stress/drug effects , Hypoglycemic Agents/pharmacology , Lipids , Liver/drug effects
11.
Bol. latinoam. Caribe plantas med. aromát ; 18(4): 347-358, jul. 2019. ilus
Article in English | LILACS | ID: biblio-1008172

ABSTRACT

Manilkara zapota (Sapotaceae), commonly known as Sapodilla, is widely known for its delicious fruit. Various parts of this plant are also used in folk medicine to treat a number of conditions including fever, pain, diarrhoea, dysentery, haemorrhage and ulcers. Scientific studies have demonstrated analgesic, anti-inflammatory, antioxidant, cytotoxic, antimicrobial, antidiarrheal, anti-hypercholesteremic, antihyperglycemic and hepatoprotective activities in several parts of the plant. Phytochemical studies have revealed the presence of phenolic compounds including protocatechuic acid quercitrin, myricitrin, catechin, gallic acid, vanillic acid, caffeic acid, syringic acid, coumaric acid, ferulic acid, etc. as main constituents of the plant. Several fatty acids, carotenoids, triterpenes, sterols, hydrocarbons and phenylethanoid compounds have also been isolated from M. zapota. The present review is a comprehensive description focused on pharmacological activities and phytochemical constituents of M. zapota.


Manilkara zapota (Sapotaceae), comúnmente conocida como Sapodilla, es ampliamente conocida por su delicioso fruto. Variadas partes de esta planta se usan en medicina popular para tratar una serie de afecciones, como fiebre, dolor, diarrea, disentería, hemorragia y úlceras. Estudios científicos han demostrado actividad analgésica, antiinflamatoria, antioxidante, citotóxica, antimicrobiana, antidiarreica, antihipercolesterolémica, antihiperglucémica y hepatoprotectora en diferentes partes de la planta. Los estudios fitoquímicos han revelado la presencia de compuestos fenólicos que incluyen ácido protocatechúico, quercitrina, miricitrina, catequina, ácido galico, ácido vanílico, ácido cafeico, ácido sirínico, ácido cumárico, ácido fúnico y ácido ferúlico como componentes principales de la planta. Varios ácidos grasos, carotenoides, triterpenos, esteroles, hidrocarburos y compuestos feniletanoides también han sido aislados de M. zapota. La presente revisión es una descripción exhaustiva centrada en las actividades farmacológicas y los constituyentes fitoquímicos de M. zapota.


Subject(s)
Plant Extracts/pharmacology , Manilkara/chemistry , Phenols/isolation & purification , Phenols/pharmacology , Saponins/isolation & purification , Saponins/pharmacology , Sterols/isolation & purification , Sterols/pharmacology , Triterpenes/isolation & purification , Triterpenes/pharmacology , Carotenoids/isolation & purification , Carotenoids/pharmacology , Sapotaceae/chemistry , Phenolic Compounds/analysis , Medicine, Traditional
12.
Electron. j. biotechnol ; 38: 27-31, Mar. 2019. graf, ilus
Article in English | LILACS | ID: biblio-1051305

ABSTRACT

BACKGROUND: Oral cancer is one of the common malignant tumors of the head and neck. However, current treatments have numerous side effects, and drugs from natural sources may have better therapeutic potential. This research investigated the induction of apoptosis by α-hederin (α-HN), a constituent of Pulsatilla chinensis (Bunge) Regel, in the oral cancer cell line SCC-25 and its underlying mechanism. RESULTS: SCC-25 cells were treated with 50, 100, and 200 µmol/L α-HN. Cell proliferation; extent of apoptosis; activities of caspases-3, 8, and 9; and the expression of Bcl-2, Bax, phosphorylated (p)-phosphoinositide 3-kinase (PI3K), p-Akt, and p-mammalian target of rapamycin (mTOR) proteins were determined using the 3-(4,5)-2-thiazole-(2,5)-diphenyl tetrazolium bromide, flow cytometry, caspase activity detection kits, and western blot assays, respectively. The results showed that the proliferation of SCC-25 cells in the α-HN-treated groups decreased significantly, and the inhibitory effect was time and concentration dependent. Compared with cells in the control group, the extent of apoptosis increased significantly, caspase-3 and -9 activities were significantly enhanced, and the Bcl-2 level was lowered and the Bax level was elevated significantly in SCC-25 cells treated with α-HN for 48 h (P b 0.05). The expression of p-PI3K, p-Akt, and p-mTOR was also significantly lower in SCC-25 cells treated with α-HN than that in the control group (P b 0.05). CONCLUSION: These results indicate that α-HN can inhibit proliferation and induce apoptosis of SCC-25 cells and may exert these effects by inhibiting the PI3K/Akt/mTOR signaling pathway.


Subject(s)
Oleanolic Acid/analogs & derivatives , Saponins/pharmacology , Mouth Neoplasms/metabolism , Apoptosis/drug effects , Oleanolic Acid/metabolism , Oleanolic Acid/pharmacology , Saponins/metabolism , Signal Transduction/drug effects , Cell Survival , Blotting, Western , Phosphatidylinositol 3-Kinases/metabolism , Caspases , Pulsatilla , Cell Proliferation/drug effects , Proto-Oncogene Proteins c-akt/metabolism , TOR Serine-Threonine Kinases/metabolism , Flow Cytometry , Head and Neck Neoplasms/metabolism
13.
Braz. oral res. (Online) ; 33: e032, 2019. tab, graf
Article in English | LILACS | ID: biblio-1001608

ABSTRACT

Abstract: This study aimed to investigate the effects of astragaloside IV (AsIV) on inflammation and immunity in rats with experimental periodontitis. Periodontitis was established in 48 Wistar rats, which were then randomly divided into model and 10, 20 and 40 mg/kg AsIV groups, with 12 rats in each group. The latter 3 groups were treated with AsIV at doses of 10, 20 and 40 mg/kg, respectively. The control group (12 rats, without periodontitis) and model group were given the same amount of 5% sodium carboxymethyl cellulose. The treatment was performed once per day for 8 weeks. Before and after treatment, the tooth mobility scores of the rats were determined. After treatment, the salivary occult blood index (SOBI), plaque index (PLI), peripheral blood T lymphocyte subsets, and serum inflammatory factor and immunoglobulin levels were determined. The results showed that, after treatment, compared with that in model group, in 40 mg/kg AsIV group, the general state of rats was improved, while the tooth mobility score, SOBI and PLI were significantly decreased (p < 0.05); the peripheral blood CD4+ T cell percentage and CD4+/CD8+ ratio were significantly increased (p < 0.05), while the CD8+ T cell percentage was significantly decreased (p < 0.05); the serum tumor necrosis factor-α, interleukin-1β and interleukin-2 levels were significantly decreased (p < 0.05); the serum immunoglobulin A and immunoglobulin G levels were significantly decreased (p < 0.05). In conclusion, AsIV can alleviate inflammation and enhance immunity in rats with experimental periodontitis.


Subject(s)
Animals , Male , Female , Periodontitis/drug therapy , Saponins/pharmacology , Triterpenes/pharmacology , Immune System/drug effects , Periodontitis/immunology , Periodontitis/pathology , Reference Values , Tooth Mobility , Immunoglobulins/blood , Random Allocation , Reproducibility of Results , T-Lymphocyte Subsets , Interleukin-2/blood , Tumor Necrosis Factor-alpha/blood , Treatment Outcome , Rats, Wistar , Interleukin-1beta/blood
14.
Acta cir. bras ; 34(7): e201900708, 2019. tab, graf
Article in English | LILACS | ID: biblio-1038121

ABSTRACT

Abstract Purpose: To investigate the effect of astragaloside IV (As-IV) on myocardial ischemia-reperfusion (I/R) injury in rats and reltaed mechanisms. Methods: Sixty rats were randomly divided into sham-operated, control I/R and 2.5, 5 and 10 mg/kg As-IV groups, 12 rats in each group. The later three groups were intragastrically administered with As-IV for 7 days, with a dose of 2.5, 5 and 10 mg/kg, respectively. The myocardial I/R injury model was constructed in later four groups. At the end of reperfusion, the cardiac function indexes, serum lactate dehydrogenase (LDH) and creatine kinase (CK) levels, heart weight (HW)/body weight (BW) ratio and infarct size, and expressions of phosphatidylinositol-3 kinase/serine-threonine protein kinase (PI3K/AKT) and glycogen synthase kinase-3β (GSK-3β) proteins and the phosphorylated forms (p-AKT, p-GSK-3β) were determined. Results: Compared with control I/R group, in 5 and 10 mg/kg As-IV groups the left ventricular systolic pressure, fractional shortening and ejection fraction were increased, the left ventricular end-diastolic pressure was decreased, the serum LDH and CK levels were decreased, the HW/BW ratio and myocardial infarct size were decreased, and the p-Akt/Akt ratio and p-GSK-3β/GSK-3β ratio were increased (all P < 0.05). Conclusion: As-IV can alleviate the myocardial I/R injury in rats through regulating PI3K/AKT/GSK-3β signaling pathways.


Subject(s)
Animals , Male , Rats , Saponins/pharmacology , Triterpenes/pharmacology , Myocardial Reperfusion Injury/prevention & control , Signal Transduction/drug effects , Phosphatidylinositol 3-Kinases/metabolism , Proto-Oncogene Proteins c-akt/metabolism , Glycogen Synthase Kinase 3 beta/metabolism , Phosphorylation , Myocardial Reperfusion Injury/drug therapy , Rats, Sprague-Dawley
15.
Biol. Res ; 49: 1-9, 2016. ilus, graf, tab
Article in English | LILACS | ID: biblio-950845

ABSTRACT

BACKGROUND: Cancer, being the foremost challenge of the modern era and the focus of world-class investigators, gargantuan research is in progress worldwide to explore novel therapeutic for its management. The exploitation of natural sources has been proven to be an excellent approach to treat or minify the excessive angiogenesis and proliferation of cells. Similarly, based the ethnomedicinal uses and literature survey, the current study is designed to explore the anti-tumor and anti-angiogenic potentials of Rumex hastatus. Anti-tumor and anti-angiogenic activities were carried out using potato-disc model and chorioallantoic membrane (CAM) assay respectively. Moreover, R. hastatus was also assessed for antibacterial activity against Agrobacterium tumefaciens (tumor causing bacterial strain). The positive controls used in anti-tumor, anti-angiogenic and antibacterial activities were vincristine sulphate, dexamethasone and cefotaxime respectively. RESULTS: The crude saponins (Rh.Sp), methanolic extract (Rh.Cr) and other solvent extracts like n-hexane (Rh.Hex), chloroform (Rh.Chf), ethylacetate (Rh.EtAc) and aqueous fraction (Rh.Aq) exhibited notable anti-tumor and anti-angiogenic activities. In potato tumor assay, the chloroform and saponin fractions were observed to be the most effective showing 86.7 and 93.3 % tumor inhibition at 1000 µg/ml with IC50 values 31.6 and 18.1 µg/ml respectively. Similarly, these two samples i.e., chloroform and saponins also excelled among the entire test samples in anti-angiogenic evaluation exhibiting 81.6 % (IC50 = 17.9 µg/ml) and 78.9 % (IC50 = 64.9 µg/ml) at 1000 µg/ml respectively. In contrast, the antibacterial investigations revealed a negligible potential against A. tumefaciens. CONCLUSION: Based on our results we can claim that R. hastatus possesses both anti-tumor and anti-angiogenic potentials. In all of the solvent fractions, Rh.Chf and Rh.Sp were most effective against tumor and angiogenesis while having negligible activity against A. tumefaciens. It can be concluded that Rh.Chf and Rh.Sp might be potential targets in the isolation of natural product having anti-neoplastic action.


Subject(s)
Saponins/pharmacology , Plant Extracts/pharmacology , Agrobacterium tumefaciens/drug effects , Angiogenesis Inhibitors/pharmacology , Rumex/chemistry , Antineoplastic Agents/pharmacology , Plant Tumors , Saponins/isolation & purification , Solvents/chemistry , Time Factors , Microbial Sensitivity Tests , Reproducibility of Results , Analysis of Variance
16.
European J Med Plants ; 2014 Dec; 4(12): 1400-1407
Article in English | IMSEAR | ID: sea-164206

ABSTRACT

Albizia lebbeck is a tree widely distributed in India and is also found in South Africa, South America and Australia. As in Indian traditional system as folk medicine, this plant is used to treat several inflammatory pathologies such as asthma, arthritis and burns. Study of other species this same genus has demonstrated an anti-inflammatory activity of crude extract, which, in some work has been attributed to the presence of saponins. In order to confirm these findings a study of phytochemical profile was realized and a rich extract in saponins, butanolic extract, was obtained and its anti-inflammatory activity was evaluated through measured by inhibition of carrageenan-induced mouse paw oedema, using dexamethasone as reference compound. The extract exhibited a moderate control of the both phase of inflammation, provoking an inhibition of edema formation. However, the butanolic extract exhibited lesser activity than reference compound dexamethasone. The results obtained suggest a significant antiinflamatory property of the butanolic extract of Albizia lebbeck, justifying the use of this plant in the traditional medicine for the treatment of inflammatory conditions and confirm their saponins as bioactive product.


Subject(s)
Albizzia/classification , Albizzia/pharmacology , Albizzia/therapeutic use , Butanols/pharmacology , Butanols/therapeutic use , Humans , Anti-Inflammatory Agents , India , Medicine, Traditional , Plant Extracts , Saponins/pharmacology , Saponins/therapeutic use
17.
Braz. j. med. biol. res ; 46(8): 670-675, ago. 2013. tab, graf
Article in English | LILACS | ID: lil-684531

ABSTRACT

Ziyuglycoside II is an active compound of Sanguisorba officinalis L. that has anti-inflammation, antioxidation, antibiosis, and homeostasis properties. We report here on the anticancer effect of ziyuglycoside II on human gastric carcinoma BGC-823 cells. We investigated the effects of ziyuglycoside II on cell growth, cell cycle, and cell apoptosis of this cell line. Our results revealed that ziyuglycoside II could inhibit the proliferation of BGC-823 cells by inducing apoptosis but not cell cycle arrest, which was associated with regulation of Bax/Bcl-2 expression, and activation of the caspase-3 pathway. Our study is the first to report the antitumor potential of ziyuglycoside II in BGC-823 gastric cancer cells. Ziyuglycoside II may become a potential therapeutic agent against gastric cancer in the future.


Subject(s)
Humans , Apoptosis/drug effects , /metabolism , /metabolism , Saponins/pharmacology , Signal Transduction/drug effects , /metabolism , Antineoplastic Agents/pharmacology , Cell Line, Tumor , Carcinoma/drug therapy , /drug effects , Caspase Inhibitors/metabolism , Cell Cycle/drug effects , Cell Proliferation/drug effects , Fluorometry , Fluorouracil/pharmacology , /drug effects , Sanguisorba/chemistry , Stomach Neoplasms/drug therapy , /drug effects
18.
Bol. latinoam. Caribe plantas med. aromát ; 10(6): 491-499, ene. 2011. ilus
Article in Portuguese | LILACS | ID: lil-618845

ABSTRACT

To focus on the current evidence on the anxiolytic activity of Panax ginseng C.A Meyer. Recent studies showed the anxiolytic effects of the constituents of the roots of this species. Triterpenoid saponins of ginseng known as ginsenosides, are the active chemical components of the roots of this plant likely related to its anxiolytic activity. The interaction of these components with ligands of GABA receptor, increasing its affinity for the receptor, decreased production of mRNA catabolic enzyme (Abat) and this inhibitory neurotransmitter GABA transporter (GAT1) are related events so far to the anxiolytic effect of ginseng.Both the white ginseng and red ginseng have anxiolytic properties.


Estudo enfoca as atuais evidências sobre a atividade ansiolítica do Panax ginseng C.A Meyer. Recentes pesquisas evidenciaram os efeitos ansiolíticos dos constituintes das raízes desta espécie. Saponinas triterpenóides de ginseng, conhecidos como ginsenosídeos, são os componentes químicos ativos das raízes desta planta relacionados à sua provável atividade ansiolítica. A interação destes constituintes com ligantes do receptor GABA, aumentando a sua afinidade pelo receptor, a diminuição da produção de RNAm da enzima catabólica (Abat) deste neurotransmissor inibitório e do transportador GABA (GAT1) são eventos relacionados até o momento ao efeito ansiolítico do ginseng. Tanto o ginseng branco como o ginseng vermelho apresentam propriedades ansiolíticas.


Subject(s)
Anxiety/drug therapy , Anti-Anxiety Agents/pharmacology , Ginsenosides/pharmacology , Panax/chemistry , gamma-Aminobutyric Acid , Plant Roots/chemistry , Saponins/pharmacology
19.
Article in English | WPRIM | ID: wpr-127694

ABSTRACT

Early diabetic nephropathy is characterized by glomerular hyperpermeability as a result of impaired glomerular filtration structure caused by hyperglycemia, glycated proteins or irreversible advanced glycosylation endproducts (AGE). To investigate the effect of ginseng total saponin (GTS) on the pathologic changes of podocyte ZO (zonula occludens)-1 protein and podocyte permeability induced by diabetic conditions, we cultured mouse podocytes under: 1) normal glucose (5 mM, = control); 2) high glucose (HG, 30 mM); 3) AGE-added; or 4) HG plus AGE-added conditions and treated with GTS. HG and AGE increased the dextran filtration of monolayered podocytes at early stage (2-8 hr) in permeability assay. In confocal imaging, ZO-1 colocalized with actin filaments and beta-catenin at cell contact areas, forming intercellular filtration gaps. However, these diabetic conditions suppressed ZO-1 immunostainings and disrupted the linearity of ZO-1. In Western blotting, diabetic conditions also decreased cellular ZO-1 protein levels at 6 hr and 24 hr. GTS improved such quantitative and qualitative changes. These findings imply that HG and AGE have an influence on the redistribution and amount of ZO-1 protein of podocytes thereby causing hyperpermeability at early stage, which can be reversed by GTS.


Subject(s)
Actin Cytoskeleton/metabolism , Animals , Cell Line , Diabetic Nephropathies/physiopathology , Glomerular Filtration Rate , Glucose/pharmacology , Glycation End Products, Advanced/pharmacology , Hyperglycemia/physiopathology , Membrane Proteins/metabolism , Mice , Panax , Permeability/drug effects , Phosphoproteins/metabolism , Plant Preparations/pharmacology , Podocytes/drug effects , Saponins/pharmacology , beta Catenin/metabolism
20.
Rev. biol. trop ; 56(4): 1645-1652, Dec. 2008. ilus, tab
Article in Spanish | LILACS | ID: lil-637769

ABSTRACT

Steroidal saponins from the plant Agave brittoniana with activity against the parasite Trichomona vaginalis. The genus Agave (Agavaceae), includes more than 300 species; around 16 of them show an homogeneous distribution throughout Cuba. Agave brittoniana (ssp. brachypus), is an endemic subspecies that grows in the central region of the country and its leaves are traditionally used in the treatment of parasitic diseases. The parasite Trichomonas vaginalis causes the disease known as trichomoniasis, that infects the genital tract. To test in vitro the plant against Trichomona vaginalis, the dried and powdered leaves were extracted three times with ethanol-water (7 : 3) by maceration at room temperature. The solvent was removed under reduced pressure and the extract was suspended in distilled water, defatted with n-hexane, and extracted with water-saturated n-butanol. After solvent removal, a portion of the n-butanol extract was hydrolyzed. After extraction with ethyl acetate the hydrolysis products were compared with authentic sapogenins samples using thin layer chromatography (TLC). Most of the sapogenins (yuccagenin and diosgenin) were isolated and their structures were confirmed. using nuclear magnetic resonance (NMR) experiments. The n-butanol extract was subjected to a separation process through column chromatography to obtain five fractions. After multiple separation processes by reversed phase high performance liquid chromatography (HPLC), the most active one produced one refined fraction that contained two saponins with the same aglycone (diosgenin) and one yuccagenin based saponin. Best results of the activity were obtained with the yuccagenin derived glycoside. Rev. Biol. Trop. 56 (4): 16451652. Epub 2008 December 12.


El género Agave, familia Agavaceae, tiene más de 300 especies, con aproximadamente 16 distribuidas en toda Cuba. Una de ellas, el Agave brittoniana Trel. (ssp. brachypus), es una subespecie endémica y sus hojas son tradicionalmente utilizadas en el tratamiento de enfermedades parasitarias. Se realizaron estudios "in vitro" de la actividad de productos de esta planta frente a Trichomona vaginalis. Las hojas secas y pulverizadas fueron extraídas tres veces con una mezcla de etanol-agua (7: 3) mediante maceración a temperatura ambiente. El disolvente fue evaporado a presión reducida y el extracto fue suspendido en agua destilada, desengrasado con n-hexano, y extraído con n-butanol saturado con agua. Luego de una extracción con acetato de etilo, los productos de la hidrólisis fueron comparados con patrones de sapogeninas mediante la cromatografía de capa fina (CCD). Aislamos las sapogeninas mayoritarias (yuccagenina y diosgenina) y confirmamos sus estructuras utilizando técnicas de resonancia magnética nuclear. Por otra parte, el extracto n-butanólico fue sometido a un proceso de separación biodirigido mediante cromatografía de columna, obteniéndose cinco fracciones. Después de múltiples separaciones, la más activa rindió una fracción purificada con dos sapogeninas con el mismo aglicón (diosgenina) y un glicósido de yucagenina. Los mejores resultados de esta actividad fueron obtenidos con el glicósido derivado de la yucagenina.


Subject(s)
Animals , Agave/chemistry , Antiprotozoal Agents/pharmacology , Plant Extracts/pharmacology , Saponins/pharmacology , Trichomonas vaginalis/drug effects , Antiprotozoal Agents/chemistry , Antiprotozoal Agents/isolation & purification , Parasitic Sensitivity Tests , Saponins/chemistry , Saponins/isolation & purification
SELECTION OF CITATIONS
SEARCH DETAIL