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1.
Article in English | WPRIM | ID: wpr-878444

ABSTRACT

OBJECTIVES@#This study investigated the effects of different implant surface properties on the biological behavior of Schwann cells.@*METHODS@#Schwann cells (SCs) were cultured on three types of implant surfaces including smooth polished (SMO), sand-blasted, large grit, acid-etched (SLA), and chemically-modified SLA (modSLA). At different time points, the morphology and adhesion of SCs on the implant surfaces were observed by scanning electron microscope. Cell proliferation activity was detected by MTT method. The expression levels of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) were detected by enzyme-linked immunosorbent assay. Changes in the mRNA levels of NGF and BDNF were detected by real-time fluorescent quantitative polymerase chain reaction (PCR).@*RESULTS@#SCs adhered, stretched, and proliferated well on the three types of implant surfaces. On the 3rd, 5th, and 7th days, the OD values of the SMO group were higher than those of the SLA group and the modSLA group, and the difference was statistically significant (@*CONCLUSIONS@#Different implant surface properties have different effects on the biological behavior of SCs. Proliferation of SCs is significantly promoted by smooth surface, while secretion and gene expression of neurotrophic factors are significantly promoted by modSLA surface at early stage.


Subject(s)
Dental Implants , Schwann Cells , Surface Properties , Titanium
2.
Rev. argent. mastología ; 39(144): 12-19, sept. 2020. ilus
Article in Spanish | LILACS, BINACIS | ID: biblio-1150803

ABSTRACT

Introducción: El schwannoma (neurinoma o neurilemoma) es un tumor benigno originado en la vaina de mielina de los nervios periféricos a partir de la células de Schwann. En su variedad benigna es el tumor más frecuente dependiente de esta estructuras. Se manifiesta entre la tercera y quinta década de vida, sin distinción de género. La localización axilar es extremadamente infrecuente, constituyendo el 5% de todos los casos reportados. Los schwannomas son tumores bien delimitados y de lento crecimiento. La presentación más frecuente es como masa palpable o por la sintomatología clínica correspondiente al territorio de inervación del nervio afectado. Es importante tener en cuenta que estas lesiones puedes formar parte de cuadros clínicos de base genética más complejos como la neurofibromatosis, entre otros. El método diagnóstico de elección es la resonancia magnética nuclear. El tratamiento consiste en la extirpación de la lesión tratando de preservar la función de la estructura nerviosa afectada. Objetivo: El objetivo del presente trabajo es realizar el reporte de un caso de lesión compatible con schwannoma axilar y realizar un revisión de la literatura.


Introduction: Schwannoma (neurinoma or neurilemoma) is a benign tumor originated in myelin sheath of peripheral nerves from schwann cells. In its benign variety, it is the most frequent tumor dependent of these structures. It appears between the third and fifth decade of life without distinction of geder. Axillary location is extremely rare, accounting for 5% of all reported cases. Schwqnnomas are well-defide, slow-growing tumors. The most frequent presentation is as palpabel mass or due to the clinical symptoms corresponding to the innervation territory of the affected nerve. It's important to know that these lesions can be part of more complex genetic-based clinical cases such as neurofibromatosis. The diagnostic method of choice is magnetic resonance imaging. Treatment cosists of excising the lesion, trying to preserve the function of the affected nerve structure. Objetive: The aim of this report is to describe our experience with one case of axillary schwannoma diagnosed in our institution and to perform a review of the literature.


Subject(s)
Schwann Cells , Peripheral Nerves , Therapeutics , Magnetic Resonance Imaging , Neurofibromatoses , Neoplasms , Neurilemmoma
3.
Arch. argent. pediatr ; 118(4): e410-e413, agosto 2020. ilus
Article in Spanish | LILACS, BINACIS | ID: biblio-1118594

ABSTRACT

El schwannoma es un tumor primario, habitualmente, benigno, procedente de las células de Schwann, productoras de la vaina de mielina que recubre los nervios periféricos. Constituye menos del 10 % de los tumores intracraneales y es infrecuente en la edad pediátrica.Se presenta a un paciente de 6 años y 11 meses de edad, previamente sano, con antecedente de cefalea holocraneana intermitente asociado a proptosis y disminución de la agudeza visual del ojo izquierdo, epífora y estrabismo, con evidencia tomográfica de una masa retroocular. Se realizó la exéresis macroscópicamente completa, con diagnóstico anatomopatológico de schwannoma orbitario


Schwannoma is a usually benign primary tumor. It develops from the Schwann cells, which produce the myelin sheath that surrounds the peripheral nerves. It represents less than 10 % of the intracranial tumors, and it is infrequent in the pediatric age.We hereby present a 6-year-and-11-month-old previously healthy patient, with a history of intermittent generalized cephalea associated with proptosis and a diminished visual acuity of the left eye, epiphora and strabismus, with radiological evidence of retro-ocular mass. A macroscopically complete exeresis was performed, with an anatomopathological diagnosis of orbital schwannoma


Subject(s)
Humans , Male , Child , Schwann Cells , Neurilemmoma/diagnostic imaging , Orbit/injuries , Exophthalmos , Neoplasms , Neurilemmoma/surgery
4.
Mem. Inst. Oswaldo Cruz ; 115: e200075, 2020. graf
Article in English | LILACS, SES-SP | ID: biblio-1135240

ABSTRACT

BACKGROUND Although Mycobacterium leprae (ML) is well characterised as the causative agent of leprosy, the pathophysiological mechanisms underlying peripheral nerve damage still need further understanding. In vitro and in vivo studies have yielded insights into molecular mechanisms of ML interaction with Schwann cells (SC), indicating the regulation of genes and proteins crucial to neural plasticity. OBJECTIVES We aimed to investigate the effect of ML on neurotrophins expression in human SC (hSC) and mice sciatic nerves to better understand their role in leprosy neuropathy, and aiming to contribute to future therapeutic approaches. METHODS We evaluated mRNA and protein expression of BDNF, NGF, NT-3, NT-4 in hSC from amputation nerve fragments, as well as in athymic nude mice, infected by ML for eight months. FINDINGS and MAIN CONCLUSIONS Our in vitro results showed a trend to decline in NGF and BDNF mRNA in ML-treated hSC, compared to controls. The immunodetection of BDNF and NT-4 was significantly downregulated in ML-treated hSC. Conversely, ML-infected mice demonstrated upregulation of NT-3, compared to non-infected animals. Our findings indicate that ML may be involved in neurotrophins regulation, suggesting that a pathogen-related imbalance of these growth factors may have a role in the neural impairment of leprosy.


Subject(s)
Humans , Animals , Mice , Schwann Cells/metabolism , Sciatic Nerve/metabolism , Mycobacterium leprae , Nerve Growth Factors/metabolism , Mice, Nude
5.
Rio de Janeiro; s.n; 2020. xii, 79 p. ilus.
Thesis in Portuguese | LILACS | ID: biblio-1129385

ABSTRACT

A hanseníase é uma doença infecciosa, desmielinizante, que pode levar à incapacidades e deformidades físicas permanentes. Dados anteriores demonstraram que Mycobacterium leprae, o agente etiológico da doença, consegue modular a biologia das células de Schwann. Um ponto ainda não elucidado é se o dano neural na hanseníase é causado diretamente pelo bacilo ou se é dependente do infiltrado inflamatório. Sabe-se que durante os episódios reacionais, o aumento de citocinas pró-inflamatórias, como IFN-γ, pode contribuir para o incremento do dano neural por um mecanismo ainda desconhecido e, uma correlação positiva entre concentrações séricas de TNF-α e desmielinização em pacientes com hanseníase já foi demonstrada. IFN-γ e TNF-α são citocinas capazes de induzir a expressão de Indoleamina 2,3 dioxigenase (IDO1), enzima chave na regulação da via das quinureninas, em diversos tipos celulares como monócitos, macrófagos e células dendríticas. Diversos estudos envolvendo patologias do sistema nervoso central apontam que as quinureninas produzidas pela degradação do triptofano podem ter uma função neurotóxica, mas pouco se sabe sobre o envolvimento de IDO1 e seus metabólitos na patogênese de doenças do sistema nervoso periférico. O presente estudo teve como objetivo avaliar a possível contribuição de IDO1 e seus metabólitos na neuropatia hanseniana. Inicialmente foi demonstrado que M. leprae aumenta a expressão de IDO1 em células de Schwann primárias e da linhagem ST88-14. No entanto, o bacilo não é capaz de aumentar a atividade de IDO1 em células de Schwann da linhagem ST88-14.


Foi observado que as citocinas pró-inflamatórias IFN-γ e TNF-α isoladamente não são capazes de induzir a expressão proteica de IDO1, mas aumentam significativamente sua atividade em células ST88-14. Em associação com M. leprae, IFN-γ exerce um forte estímulo na indução da atividade enzimática de IDO1. A infecção com M. leprae diminui a expressão gênica de Quinurenina aminotransferase II (AADAT), sugerindo que o mesmo possa estar direcionando a via para a produção de metabólitos neurotóxicos como o ácido quinolínico (QUINA), 3-hidroxiquinurenina (3-HK) e ácido 3-hidroxiantranílico (3HAA). A análise de viabilidade celular demonstrou que 3-HAA é capaz de induzir apoptose em células ST88-14 e, a análise por ELISA, dos sobrenadantes de 24h de cultura, demonstrou que M. leprae viável induz aumento na concentração do metabólito ácido quinurênico (KYNA) que é significativamente reduzida na presença de IFN-γ, enquanto IFN-γ aumenta significativamente a concentração de QUINA, principalmente quando associado à M. leprae morto. Foi observado um aumento na atividade de IDO1 em soro de pacientes com neuropatia hanseniana em relação a pacientes com outras neuropatias periféricas. Uma correlação positiva foi observada entre a atividade de IDO1 e a gravidade do dano neural, conforme avaliado pelo exame de eletroneuromiografia. Em conjunto, os dados apresentados sugerem o envolvimento da via das quinureninas no dano neural na hanseníase. (AU)


Subject(s)
Humans , Schwann Cells , Indoleamine-Pyrrole 2,3,-Dioxygenase , Kynurenine , Leprosy , Mycobacterium leprae
6.
Article in Chinese | WPRIM | ID: wpr-826528

ABSTRACT

Charcot-Marie-Tooth disease (CMT) is the commonest form of inherited neuropathy and has an incidence of 1/2500. CMT1A is the commonest subtype of CMT, which is caused by duplication of peripheral myelin protein 22 (PMP22) gene and accounts for approximately 50% of CMT diagnosed by genetic testing. Duplication of PMP22 may influence the production of PMP22 mRNA and protein, and interfere with the proliferation, differentiation and apoptosis of Schwann cells. In addition, deregulation of NRG1/ErbB pathway and lipid metabolism can also lead to dysfunction of Schwann cells. Such factors may disturb the myelination process, leading to axon degeneration, muscle weakness, and atrophy subsequently. Accordingly, drug therapies for CMT1A are developed by targeting such factors. PXT3003, antisense oligonucleotides (ASOs) and small interfering RNA (siRNA) are supposed to down-regulate the level of PMP22 mRNA, while recombinant human NRG-1 (rhNRG1) and neurotrophin-3 (NT-3) may enhance Schwann cells survival and differentiation. In addition, lipid-supplemented diet may remedy the defect of lipid metabolism and maintain the proper structure of myelin. Other targeting drugs include ascorbic acid, progesterone antagonists, IFB-088, ADX71441, and ACE-083. This review is to sum up the pathogenesis of CMT1A and promising targeting drug therapies for further research.


Subject(s)
Cell Differentiation , Charcot-Marie-Tooth Disease , Genetics , Pathology , Therapeutics , Genetic Testing , Humans , Schwann Cells , Cell Biology
7.
Autops. Case Rep ; 9(3): e2019099, July-Sept. 2019. ilus, tab
Article in English | LILACS | ID: biblio-1020994

ABSTRACT

Granular cell tumor (GCT) is a rare soft tissue neoplasm of Schwann cell origin. Most cases occur in adults; however, the precise incidence is unknown in children. GCT is usually a slow-growing, painless tumor involving the skin and soft tissues that is mostly located in the head and neck region, especially the tongue. The breast is one of the least common sites involved by GCT. This paper presents a 3-year-old girl who presented with a soft to firm, ill-defined swelling on the right breast with painful ulceration of the overlying skin. Fine needle aspiration rendered an initial diagnosis of fibrocystic change accompanied by apocrine metaplasia. Histologic evaluation of the excised breast mass revealed a benign granular cell tumor. Although rare, GCT of the breast should be included in the differential diagnosis for breast masses in pediatric patients. Proper diagnosis and timely management of this tumor are essential because of its malignant potential (<2% of cases) and high rate of local recurrence if not properly excised.


Subject(s)
Humans , Female , Child, Preschool , Breast Neoplasms/pathology , Granular Cell Tumor/pathology , Schwann Cells/pathology , S100 Proteins
8.
Article in English | WPRIM | ID: wpr-785875

ABSTRACT

Schwannoma or neurilemmoma is a benign peripheral nerve sheath tumor that arises from Schwann cells. Approximately 25–45% of all schwannomas occur in the head and neck regions, and the intraoral presentation of these is only 1%. We report a rare case of a patient presenting tongue base schwannoma with characteristic imaging features on computed tomography and magnetic resonance imaging.


Subject(s)
Diagnosis, Differential , Head , Humans , Magnetic Resonance Imaging , Neck , Nerve Sheath Neoplasms , Neurilemmoma , Peripheral Nerves , Schwann Cells , Tongue
10.
Article in Chinese | WPRIM | ID: wpr-773548

ABSTRACT

OBJECTIVE@#To investigate the formation of gap junctions between Schwann cells derived from differentiated adipose stem cells implanted in a rat model of dyskinesia induced by brain injury and its positive effect in promoting functional recovery of the rats.@*METHODS@#In a rat model of hemiplegia induced by motor cortex injury, adipose stem cells or Schwann cells differentiated from adipose stem cells, either with or without RNAi-mediated silencing of Cx43, were transplanted orthotopically in the lesion. The recovery of the motor function of the rats was observed and scored after the transplantation. Rat brain tissues were sampled to detect the expressions of nerve growth factor (NGF) using Western blotting and RT-PCR.@*RESULTS@#All the 3 cell transplantation therapies obviously improved the motor function scores of the rats as compared with the control rats. The expression of NGF in the brain tissue was significantly lower in the control group than in the cell transplantation groups. NGF expression in the brain tissues of rats receiving transplantation of Schwann cells with Cx43 gene silencing was lower than that in rats receiving Schwann cells without Cx43 silencing, and was similar with that in rats transplanted with adipose stem cells. The results of RT-PCR showed that NGF mRNA level in the control group was significantly lower than that in the other 3 groups. NGF mRNA expression was the highest in Schwann cell group without Cx43 silencing, followed by adipose stem cell group, and then by Schwann cell group with Cx43 silencing.@*CONCLUSIONS@#In the rat model of dyskinesia induced by brain injury, transplantations of adipose stem cells and adipose stem cells-derived Schwann cells both promote the functional recovery of brain damage, in which gap junction protein Cx43 plays an important role to promote functional gap junction formation possibly by enhancing NGF expression.


Subject(s)
Animals , Brain Injuries , Dyskinesias , Gap Junctions , Rats , Rats, Sprague-Dawley , Schwann Cells , Stem Cells
11.
Article in Chinese | WPRIM | ID: wpr-776547

ABSTRACT

OBJECTIVE@#To investigate the mechanism of high glucose affecting the apoptosis of schwann cells through Nox4 NADPH oxidase.@*METHODS@#The schwann cells of newborn Wistar rats were cultured in vitro. The cultured cells were divided into four groups: control group, high-glucose group, NOX4 siRNA group and control siRNA group (n=10). The WST-1 method was used to detect the cell vitality, and the DCFH-DA method was used to detect the contents of intracellular reactive oxygen free radicals (ROS). Nox4 and Caspase3 mRNA expressions were detected by real-time fluorescence quantitative RT-PCR. Nox4 and Caspase3 protein expressions were determined by Western blot.@*RESULTS@#High glucose culture up-regulated Nox4 mRNA and protein expressions of schwann cells, decreased activity of schwann cells, increased intracellular ROS content, and promoted apoptosis by increasing Caspase3 mRNA and protein expressions. NOX4 siRNA blocked the accumulation of ROS in the high glucose cultured schwann cells, and reduced the damage of glucose on cell viability, by inhibiting NOX4 gene expression. NOX4 siRNA also reduced cell apoptosis by down-regulating Caspase3 mRNA and protein expressions.@*CONCLUSION@#Nox4 was involved in the hyperglycemic-induced apoptosis of schwann cells through ROS. The regulation of Nox4 expression or function might be a new way to treat diabetic peripheral neuropathy.


Subject(s)
Animals , Apoptosis , Caspase 3 , Metabolism , Cells, Cultured , Culture Media , Glucose , NADPH Oxidase 4 , Metabolism , Rats , Rats, Wistar , Reactive Oxygen Species , Metabolism , Schwann Cells , Cell Biology , Metabolism
12.
São Paulo; HSPM; 2019.
Non-conventional in Portuguese | LILACS, ColecionaSUS, SMS-SP, HSPM-Producao, SMS-SP | ID: biblio-1280776

ABSTRACT

RESUMO O schwannoma trata-se de um raro tumor benigno originado nas células de Schwann. As células de Schwann são responsáveis pela criação de uma membrana isolante elétrica ao redor do axônio dos neurônios periféricos, a bainha de mielina. Geralmente, causam sintomas devido à compressão de estruturas vizinhas. No presente estudo, um homem de 42 anos de idade se apresentou ao ambulatório cirúrgico devido a uma tumoração bem localizada no flanco direito. O crescimento teve início três anos antes, dolorosa, sem causa aparente. A lesão foi biopsiada e a histopatologia revelou que o tumor era schwannoma benigno. Realizado a exérese do tumor sem intercorrências. Paciente evoluiu sem complicações no pós-operatório. Até a conclusão deste presente estudo, o acompanhamento no ambulatório não demonstrou recidiva, seis meses após a cirurgia. Dessa maneira, tumorações de partes moles apresentam-se como uma queixa comum. A maioria das lesões é benigna e não requer exérese, a menos que interfira com a qualidade da vida do paciente ou se o diagnóstico é incerto. É importante estar ciente de schwannomas no diagnóstico diferencial. O diagnóstico pode ser alcançado através da combinação de imagens e biópsia, mas o tratamento definitivo é feito na exérese da massa tumoral. Enfim, percebe-se a importância desse relato de caso que elucida a clínica, diagnóstico e tratamento de uma patologia rara, sendo o sétimo caso encontrado na literatura inglesa, o terceiro sintomático e o primeiro associado ao lipoma. Palavras-chave: Schwannoma. Parede abdominal. Relato de caso.


Subject(s)
Humans , Male , Female , Schwann Cells , Case Reports , Abdominal Wall
13.
Article in English | WPRIM | ID: wpr-739201

ABSTRACT

Schwannoma is a benign tumor rarely found in the head and neck and much less commonly found in the intraparotid facial nerve. It is a slow-growing encapsulated tumor originating from the Schwann cells or axonal nerve sheath. It can occur anywhere along the course of the facial nerve. Patients may present with symptoms of facial palsy, but the most common presenting symptom is an asymptomatic swelling. Diagnosis is usually difficult before surgical removal and histopathological examination. We report a rare case of intraparotid facial nerve schwannoma in a 57-year-old female who had sustained a mass of the right preauricular area for 3 years. She reported no pain or facial muscle weakness. Enhanced computed tomography findings revealed the impression of pleomorphic adenoma. However, intraoperative gross findings were not characteristic of pleomorphic adenoma, and a frozen biopsy was performed resulting in the impression of a nerve sheath tumor. We performed an extracapsular surgical excision without parotidectomy. Permanent histopathology and immunohistochemistry reports diagnosed the mass as schwannoma. There were no complications including facial palsy after surgery. No recurrence was found at 6 months after surgery


Subject(s)
Adenoma, Pleomorphic , Axons , Biopsy , Diagnosis , Facial Muscles , Facial Nerve , Facial Paralysis , Female , Head , Humans , Immunohistochemistry , Middle Aged , Neck , Neurilemmoma , Parotid Gland , Recurrence , Schwann Cells
14.
Clinical Endoscopy ; : 382-386, 2019.
Article in English | WPRIM | ID: wpr-763447

ABSTRACT

A gangliocytic paraganglioma is a benign tumor of the digestive system with a very low incidence. The tumor is histopathologically characterized by a triphasic pattern consisting of epithelioid, ganglion, and spindle-shaped Schwann cells. In most cases, it occurs in the second portion of the duodenum near the ampulla of Vater. We report a case of a gangliocytic paraganglioma occurring at the minor duodenal papilla (a rare location) with a concurrent adenoma of the ampulla of Vater. Both lesions were treated simultaneously using endoscopic resection. Additionally, we have presented a literature review.


Subject(s)
Adenoma , Ampulla of Vater , Digestive System , Duodenum , Ganglion Cysts , Incidence , Pancreatic Ducts , Paraganglioma , Schwann Cells
15.
Article in English | WPRIM | ID: wpr-741740

ABSTRACT

Schwannomas are neurogenic tumors, which are among the most varied tumors with respect to morphology, clinical associations, and presentations; they occur in a wide variety of sites. The uterine cervix is a rare site of occurrence and only 15 cases of schwannomas of the uterine cervix, including 5 benign and 10 malignant cases, have been reported to date. Thus, schwannomas of the uterine cervix may pose diagnostic difficulty. Here, we report a case of benign schwannoma of the uterine cervix in a 37-year-old female, who presented with vaginal spotting.


Subject(s)
Adult , Cervix Uteri , Female , Humans , Metrorrhagia , Nerve Sheath Neoplasms , Neurilemmoma , Polyps , Schwann Cells
16.
Article in Korean | WPRIM | ID: wpr-760102

ABSTRACT

Schwannoma is a benign nerve sheath tumor composed of schwann cells. Most schwannoma arising in the middle ear are facial nerve schwannoma. In very rare occasions, schwannoma of the middle ear can arise from chorda tympani nerve. Hearing loss and tinnitus are the most common symptoms of patients with schwannoma of chorda tympani nerve and it can be treated by surgical excision. Recently, we treated a male patient with schwannoma of the chorda tympani nerve. This is the first case of schwannoma of the chorda tympani nerve reported in Korea. Herein, we present the case in detail with a review of the related literature.


Subject(s)
Chorda Tympani Nerve , Ear, Middle , Facial Nerve , Hearing Loss , Humans , Korea , Male , Neurilemmoma , Schwann Cells , Tinnitus
17.
Article in Korean | WPRIM | ID: wpr-786618

ABSTRACT

Gastric schwannoma, a rare mesenchymal tumor originating from the schwann cells of peripheral nerves, rarely occurs in the gastrointestinal tract. It accounts for only 0.2% of all gastric tumors and 2~6% of gastric mesenchymal tumors. Gastric schwannoma is observed as a subepithelial tumor on endoscopy; it is covered with normal mucosa, rendering its preoperative differential diagnosis difficult. An asymptomatic 43-year-old woman visited our hospital after a 7-cm ulcerofungating mass was detected in the lesser curvature of the gastric body on gastroscopy. Abdominal CT revealed multiple enlarged lymph nodes, and ¹⁸F-fluorodeoxyglucose (FDG) PET demonstrated a subtle uptake of FDG, suggestive of advanced gastric cancer. After three failed attempts of endoscopic biopsy, the patient underwent total gastrectomy with lymphadenectomy and was subsequently diagnosed with gastric schwannoma. Herein, we report this case with a literature review.


Subject(s)
Adult , Biopsy , Diagnosis, Differential , Endoscopy , Female , Gastrectomy , Gastrointestinal Tract , Gastroscopy , Humans , Lymph Node Excision , Lymph Nodes , Mucous Membrane , Neurilemmoma , Peripheral Nerves , Schwann Cells , Stomach Neoplasms , Tomography, X-Ray Computed
18.
Article in English | WPRIM | ID: wpr-758833

ABSTRACT

Previous studies have shown that the sciatic nerve has neurotrophic activity, and nerve regeneration, differentiation, and axon outgrowth can be modulated by different sciatic nerve preparations. However, numerous animals may have to be sacrificed to obtain enough sciatic nerves to make a sciatic nerve preparation. Some studies have demonstrated that the role of sciatic nerve preparations in neural differentiation depends on the neurotrophins that Schwann cells secrete, and these factors are highly conserved among different species. To reduce the use of experimental animals, in this study, we made a leachate by using the sciatic nerve of cattle and explored its effect on neuronal differentiation of rat PC12 cells (a useful model for studying neuronal differentiation). Results showed the neurite outgrowth of PC12 cells treated with the cattle sciatic nerve leachate for 3, 6, and 9 days was significantly improved, and the expressions of β3-tubulin and microtubule-associated protein 2 (two neuron-specific proteins) were increased. Moreover, the ERK1/2 signaling pathway was activated after PC12 cells were incubated with cattle sciatic nerve leachate for 9 days. Thus, a sciatic nerve leachate obtained from cattle can effectively induce neuronal differentiation of rat PC12 cells via ERK1/2 signaling pathway.


Subject(s)
Animals , Axons , Cattle , Microtubule-Associated Proteins , Nerve Growth Factors , Nerve Regeneration , Neurites , Neurons , PC12 Cells , Rats , Schwann Cells , Sciatic Nerve
19.
Clinical Endoscopy ; : 587-590, 2018.
Article in English | WPRIM | ID: wpr-717967

ABSTRACT

Schwannomas are uncommon neoplasms that arise from Schwann cells of the neural sheath. Gastrointestinal schwannomas are rare among mesenchymal tumors of the gastrointestinal tract, and only a few cases have been reported to date. Duodenal schwannomas are usually discovered incidentally and achieving a preoperative diagnosis is difficult. Schwannomas can be distinguished from other subepithelial tumors on endoscopic ultrasonography; however, any typical endosonographic features of duodenal schwannomas have not been reported due to the rarity of these tumors. Immunohistochemistry is essential to distinguish schwannomas from gastrointestinal stromal tumors and leiomyomas. We report a case of duodenal schwannoma found incidentally during a health check-up endoscopy. On endoscopic ultrasonography, this tumor was suspected as a gastrointestinal stromal tumor; therefore, the patient underwent laparoscopic wedge resection of the tumor. Histopathology and immunohistochemistry confirmed that the duodenal lesion was a benign schwannoma.


Subject(s)
Diagnosis , Duodenum , Endoscopy , Endosonography , Gastrointestinal Stromal Tumors , Gastrointestinal Tract , Humans , Immunohistochemistry , Leiomyoma , Neurilemmoma , Schwann Cells
20.
Article in English | WPRIM | ID: wpr-714995

ABSTRACT

Nerve regeneration after injury requires proper axon alignment to bridge the lesion site and myelination to achieve functional recovery. Transplanted scaffolds with aligned channels, have been shown to induce axon growth to some extent. However, the penetration of axons into the microchannels remain a challenge, influencing the functional recovery of regenerated nerves. We previously demonstrated that the size of microchannels exerts significant impact on Schwann cells (SCs) migration. Here we demonstrate that migration of SCs promotes, significantly, the dorsal root ganglion (DRG) neurons to extend axons into three-dimensional channels and form aligned fascicular-like axon tracts. Moreover, the migrating SCs attach and wrap around the aligned axons of DRG neurons in the microchannels and initiate myelination. The SCs release growth factors that provide chemotactic signals to the regenerating axons, similar to the response achieved with nerve growth factor (NGF), but with the additional capability of promoting myelination, thereby demonstrating the beneficial effects of including SCs over NGF alone in enhancing axon penetration and myelination in three-dimensional microchannels.


Subject(s)
Axons , Diagnosis-Related Groups , Ganglia, Spinal , Intercellular Signaling Peptides and Proteins , Myelin Sheath , Nerve Growth Factor , Nerve Regeneration , Neurons , Schwann Cells
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