Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 542
Filter
1.
Braz. j. biol ; 83: e249211, 2023. tab, graf
Article in English | MEDLINE, LILACS, VETINDEX | ID: biblio-1345523

ABSTRACT

Abstract This study was conducted to estimate the diversity and the occurrence of commercially important finfish species collected by twenty fish sampling site of Sindh and Baluchistan coasts of the Arabian Sea in Pakistan from January to December 2019. Additionally, physicochemical characteristics of seawater were analyzed from these selected sites and found to be within suitable ranges required for fish growth and survive. A total of 81287 fish individuals were collected and identified as 49 species belonging to 26 families in our study. The most diversified family was Sparidae (13 species) followed by Carangidae and Lutjanidae (4 species), Mullidae, Serranidae, Ariidae (3 species), and Sciaenidae (2 species). The remaining 20 families were represented by only one species. The values of Shannon diversity index calculated for the four selected habitats revealed that high fish diversity was reported at Sonmiani Coast (H'=1.81), while less at Ormara Coast (H'=0.23). Likewise, Evenness index (E) was high at Sonmiani Coast (E=0.50) and less fish diversity was reported at Ormara Coast (E=0.06). Reducing risks to threatened marine species in coastal habitats also requires conservation actions at multiple scales. Thus, it was concluded that our study could be valuable in providing the more information's regarding to the diversity of finfish species and their occurrence along the Pakistan Coast. Further, to better understand the effects, regular monitoring and conservation measures should be taken to mitigate the influence of anthropogenic activities and protect finfish diversity from further decline


Resumo Este estudo foi conduzido para estimar a diversidade e a ocorrência de espécies de peixes comercialmente importantes coletadas por vinte locais de amostragem de peixes nas costas de Sindh e Baluchistão do mar da Arábia, no Paquistão, de janeiro a dezembro de 2019. Além disso, as características físico-químicas da água do mar foram analisadas a partir desses peixes locais selecionados e considerados dentro dos intervalos adequados necessários para o crescimento e sobrevivência dos peixes. Um total de 8.1287 indivíduos de peixes foi coletado e identificado como 49 espécies pertencentes a 26 famílias em nosso estudo. A família mais diversificada foi Sparidae (13 espécies), seguida por Carangidae e Lutjanidae (4 espécies), Mullidae, Serranidae, Ariidae (3 espécies) e Sciaenidae (2 espécies). As 20 famílias restantes foram representadas por apenas uma espécie. Os valores do índice de diversidade de Shannon calculados para os quatro habitats selecionados revelaram que uma alta diversidade de peixes foi relatada na costa Sonmiani (H' = 1,81), enquanto menos na costa Ormara (H' = 0,23). Da mesma forma, o índice de regularidade (E) foi alto na costa de Sonmiani (E = 0,50) e menos diversidade de peixes foi relatada na costa de Ormara (E = 0,06). A redução dos riscos para as espécies marinhas ameaçadas em habitats costeiros também requer ações de conservação em várias escalas. Assim, concluiu-se que nosso estudo pode ser valioso para fornecer mais informações sobre a diversidade de espécies de peixes finos e sua ocorrência ao longo da costa do Paquistão. Além disso, para compreender melhor os efeitos, medidas regulares de monitoramento e conservação devem ser tomadas para mitigar a influência das atividades antropogênicas e proteger a diversidade de peixes finos de um declínio maior.


Subject(s)
Humans , Animals , Water , Biodiversity , Seawater , Ecosystem , Fishes
2.
Braz. j. biol ; 81(4): 1036-1049, Oct.-Dec. 2021. tab, graf
Article in English | LILACS | ID: biblio-1153451

ABSTRACT

Abstract The primary objective of this study was to investigate the seasonal fish and crustacean variations concerning taxonomic composition, species richness, and diversity in sandy beach habitat. For this purpose, we investigated the Sonmiani Hor lagoon area during four distinct seasons, i.e., northeast (NE) monsoon, pre-monsoon, south-west (SW) monsoon, and post-monsoon for one year. During each haul, the net was pulled about 100m along the beach in 0.5m depth. The results showed a strong linear correlation between the diversity index and equitability in fishes (r = 0.978). The diversity index was strong negatively correlated with the abundance and biomass (r = -0.978, -0.972, respectively). The physical attributes like sea surface water temperature and salinity showed a strong negative effect on species assemblages (r = -0.981 and -0.943, respectively). The mean air and water temperature illustrated approximately 3°C difference during NE and pre-monsoon seasons. However, salinity, pH, and electrical conductivity did not show any significant seasonal variabilities. Under the ecological indices, the fish species displayed higher diversity (H' = 3.19) during SW monsoon, whereas the lowest diversity was observed during pre-monsoon (H' = 1.58). The equitability and species richness, however, remained more noticeable during SW monsoon (J' = 0.81). The total number of individuals of fish and crustaceans reached 4799 with 3813 fish individuals and 986 individuals of crustaceans. A total of 27 families of fish while five crustacean families comprising of 30 genera and 38 fish species while ten genera and 17 species of crustaceans were recorded. Liza subviridis displayed the highest abundance among the sampled fish species. In conclusion, fish species constituted a significant part of the coastal fauna in the study area. The seasonal variations displayed distinct variations in fish species composition and diversity.


Resumo O objetivo principal deste estudo foi investigar as variações sazonais de peixes e crustáceos em relação à composição taxonômica, riqueza de espécies e diversidade no habitat de praias arenosas. Para esse fim, investigamos a área da lagoa Sonmiani Hor durante quatro estações distintas, ou seja, monção nordeste (NE), pré-monção, monção sudoeste (SW) e pós-monção, por um ano. Durante cada transporte, a rede foi puxada cerca de 100 m ao longo da praia, a 0,5 m de profundidade. Os resultados mostraram uma forte correlação linear entre o índice de diversidade e a equitabilidade de peixes (r = 0,978). O índice de diversidade apresentou forte correlação negativa com a abundância e a biomassa (r = -0,978 e -0,972, respectivamente). Os atributos físicos, como temperatura da água da superfície do mar e salinidade, apresentaram forte efeito negativo sobre o conjunto de espécies (r = -0,981 e -0,943, respectivamente). A temperatura média do ar e da água teve uma diferença de aproximadamente 3 °C durante a monção NE e a pré-monção. No entanto, salinidade, pH e condutividade elétrica não apresentaram variabilidade sazonal significativa. A respeito dos índices ecológicos, as espécies de peixes apresentaram maior diversidade (H' = 3,19) durante a monção SW, enquanto a menor diversidade observada foi na pré-monção (H' = 1,58). A equitabilidade e a riqueza de espécies, no entanto, permaneceram mais perceptíveis durante a monção SW (J' = 0,81). O número total de peixes e crustáceos foi de 4.799, com 3.813 peixes e 986 crustáceos. Foram registradas 27 famílias de peixes e 5 famílias de crustáceos, com 30 gêneros e 38 espécies de peixes, além de 10 gêneros e 17 espécies de crustáceos. Liza subviridis apresentou a maior abundância entre as espécies de peixes amostradas. Em conclusão, as espécies de peixes constituíram uma parte significativa da fauna costeira na área de estudo. As variações sazonais apresentaram variações distintas na composição e diversidade das espécies de peixes.


Subject(s)
Humans , Animals , Ecosystem , Fishes , Seasons , Seawater , Biodiversity , Salinity
3.
Biol. Res ; 52: 5, 2019. tab, graf
Article in English | LILACS | ID: biblio-1011408

ABSTRACT

BACKGROUND: A moderately thermophilic, slightly halophilic, aerobic, Gram-stain negative, bacterial strain, SLM16, was isolated from a mixed of seawater-sand-sediment sample collected from a coastal fumarole located in Whalers Bay, Deception Island, Antarctica. The aim was to screen for thermophilic microorganisms able to degrade primary amines and search for amine transaminase activity for potential industrial application. RESULTS: Identification and partial characterization of the microorganism SLM16 were carried out by means of morphological, physiological and biochemical tests along with molecular methods. Cells of strain SLM16 were non-motile irregular rods of 1.5-2.5 µm long and 0.3-0.45 µm wide. Growth occurred in the presence of 0.5-5.5% NaCl within temperature range of 35-55 °C and pH range of 5.5-9.5, respectively. The DNA G+C composition, estimated from ftsY gene, was 66% mol. Phylogenetic analysis using de 16S rRNA gene sequence showed that strain SLM16 belongs to the marine bacterial genus Albidovulum. CONCLUSION: Strain SLM16 is a moderate thermophilic Gram negative microorganisms which belongs to the marine bacterial genus Albidovulum and is closely related to Albidovulum inexpectatum species based on phylogenetic analysis. Additionally, amine-transaminase activity towards the arylaliphatic amine α-methylbenzylamine was detected.


Subject(s)
Seawater/microbiology , DNA, Bacterial/genetics , Rhodobacteraceae/isolation & purification , Rhodobacteraceae/enzymology , Transaminases/metabolism , Phylogeny , RNA, Ribosomal, 16S/genetics , Bacterial Typing Techniques , Sequence Analysis, DNA , Rhodobacteraceae/classification , Antarctic Regions
4.
Article in English | WPRIM | ID: wpr-786513

ABSTRACT

OBJECTIVES: Pathogenic Vibrio species are widely distributed in warm estuarine and coastal environments, and can infect humans through the consumption of raw or mishandled contaminated seafood and seawater. For this reason, the distribution of these bacteria in South Korea was investigated.METHODS: Seawater samples were collected from 145 coastal area points in the aquatic environment in which Vibrio species live. Environmental data (i.e., water temperature, salinity, turbidity, and atmospheric temperature) was collected which may help predict the distribution of the species (data not shown). Seawater samples were filtered, and incubated overnight in alkaline peptone water, at 37°C. Using species-specific polymerase chain reaction methods, screening tests were performed for the hlyA, ctxA, vvhA, and tlh genes. Clones of pathogenic Vibrio species were isolated using 3 selective plating media.RESULTS: In 2017, total seawater isolation rates for Vibrio vulnificus, Vibrio cholerae (non-pathogenic, non-O1, non-O139 serogroups), and Vibrio parahaemolyticus were 15.82%, 13.18%, 65.80%, respectively. However, in 2018 isolation rates for each were 21.81%, 19.40%, and 70.05%, respectively.CONCLUSION: The isolation rates of pathogenic Vibrio species positively correlated with the temperature of seawater and atmosphere, but negatively correlated with salinity and turbidity. From 2017 to 2018, the most frequent seawater-isolated Vibrio species were V. parahaemolyticus (68.10 %), V. vulnificus (16.54%), and non-toxigenic V. cholerae (19.58%). Comprehensive monitoring, prevention, and control efforts are needed to protect the public from pathogenic Vibrio species.


Subject(s)
Atmosphere , Bacteria , Cholera , Clone Cells , Humans , Korea , Mass Screening , Peptones , Polymerase Chain Reaction , Salinity , Seafood , Seawater , Vibrio cholerae , Vibrio parahaemolyticus , Vibrio vulnificus , Vibrio , Water
5.
Article in Korean | WPRIM | ID: wpr-759877

ABSTRACT

There are several diagnostic findings required for confirming a postmortem diagnosis of drowning. However, postmortem diagnosis of drowning remains challenging for forensic pathologists. In previous reports, several biochemical tests using various body fluids have been studied for their potential use in the postmortem diagnosis of drowning. In this study, the concentration of sodium and chloride was tested in various postmortem body fluids (vitreous humor, sphenoid sinus fluid, pleural fluid, cerebrospinal fluid, etc.) and their results were interpreted for their potential use in postmortem diagnosis of drowning. We examined 67 autopsy cases (freshwater drowning, 12 cases; seawater drowning, 16 cases; control group, 39 cases). The sodium and chloride concentration in the vitreous humor, sphenoid sinus fluid, and pleural fluid significantly correlated with each other. Furthermore, the concentrations of sodium, chloride, and the sum of the concentrations of the two in the various postmortem body fluids were significantly different in the three groups, when compared with each other (generally the concentration being the highest in the seawater drowning group, followed by the control group and the freshwater drowning group). Biochemical tests using various postmortem body fluids may serve as useful indicators for the postmortem diagnosis of drowning and for the differential diagnosis between freshwater and seawater drowning.


Subject(s)
Autopsy , Biochemistry , Body Fluids , Cerebrospinal Fluid , Diagnosis , Diagnosis, Differential , Drowning , Fresh Water , Seawater , Sodium , Sphenoid Sinus , Vitreous Body
6.
Braz. j. microbiol ; 49(4): 749-756, Oct.-Dec. 2018. tab, graf
Article in English | LILACS | ID: biblio-974295

ABSTRACT

ABSTRACT Pyrene and benzo[a]pyrene (BaP) are high molecular weight polycyclic aromatic hydrocarbons (PAHs) recalcitrant to microbial attack. Although studies related to the microbial degradation of PAHs have been carried out in the last decades, little is known about degradation of these environmental pollutants by fungi from marine origin. Therefore, this study aimed to select one PAHs degrader among three marine-derived basidiomycete fungi and to study its pyrene detoxification/degradation. Marasmiellus sp. CBMAI 1062 showed higher levels of pyrene and BaP degradation and was subjected to studies related to pyrene degradation optimization using experimental design, acute toxicity, organic carbon removal (TOC), and metabolite evaluation. The experimental design resulted in an efficient pyrene degradation, reducing the experiment time while the PAH concentration applied in the assays was increased. The selected fungus was able to degrade almost 100% of pyrene (0.08 mg mL-1) after 48 h of incubation under saline condition, without generating toxic compounds and with a TOC reduction of 17%. Intermediate metabolites of pyrene degradation were identified, suggesting that the fungus degraded the compound via the cytochrome P450 system and epoxide hydrolases. These results highlight the relevance of marine-derived fungi in the field of PAH bioremediation, adding value to the blue biotechnology.


Subject(s)
Polycyclic Aromatic Hydrocarbons/metabolism , Seawater/microbiology , Basidiomycota/metabolism , Phylogeny , Polycyclic Aromatic Hydrocarbons/chemistry , Pyrenes/metabolism , Pyrenes/chemistry , Basidiomycota/isolation & purification , Basidiomycota/classification , Basidiomycota/genetics , Benzo(a)pyrene/metabolism , Benzo(a)pyrene/chemistry , Biodegradation, Environmental , Fungal Proteins/genetics , Fungal Proteins/metabolism , Cytochrome P-450 Enzyme System/genetics , Cytochrome P-450 Enzyme System/metabolism
7.
Electron. j. biotechnol ; 33: 17-28, May. 2018. ilus, tab
Article in English | LILACS | ID: biblio-1025039

ABSTRACT

Genetic manipulation of bacteria is a procedure necessary to obtain new strains that express peculiar and defined genetic determinants or to introduce genetic variants responsible for phenotypic modifications. This procedure can be applied to explore the biotechnological potential associated with environmental bacteria and to utilize the functional properties of specific genes when inserted into an appropriate host. In the past years, marine bacteria have received increasing attention because they represent a fascinating reservoir of genetic and functional diversity that can be utilized to fuel the bioeconomy sector. However, there is an urgent need for an in-depth investigation and improvement of the genetic manipulation tools applicable to marine strains because of the paucity of knowledge regarding this. This review aims to describe the genetic manipulation methods hitherto used in marine bacteria, thus highlighting the limiting factors of the different techniques available today to increase manipulation efficiency. In particular, we focus on methods of natural and artificial transformations (especially electroporation) and conjugation because they have been successfully applied to several marine strains. Finally, we emphasize that, to avoid failure, future work should be carried out to establish tailored methodologies for marine bacteria.


Subject(s)
Seawater/microbiology , Bacteria/genetics , Genetic Engineering , Transformation, Bacterial , Genome , Electroporation , Conjugation, Genetic , Metagenomics , Single-Cell Analysis , Genetic Vectors
8.
Braz. j. microbiol ; 49(1): 10-12, Jan.-Mar. 2018. tab
Article in English | LILACS | ID: biblio-889198

ABSTRACT

ABSTRACT Vitellibacter aquimaris D-24T (=KCTC 42708T = DSM 101732T), a halophilic marine bacterium, was isolated from seawater collected from Desaru beach, Malaysia. Here, we present the draft genome sequence of D-24T with a genome size of approximately 3.1 Mbp and G + C content of 39.93%. The genome of D-24T contains genes involved in reducing a potent greenhouse gas (N2O) in the environment and the degradation of proteinaceous compounds. Genome availability will provide insights into potential biotechnological and environmental applications of this bacterium.


Subject(s)
Seawater/microbiology , Genome, Bacterial , Flavobacteriaceae/genetics , Phylogeny , Base Composition , DNA, Bacterial/genetics , Molecular Sequence Data , Base Sequence , Flavobacteriaceae/isolation & purification , Flavobacteriaceae/classification , Malaysia
9.
Braz. j. otorhinolaryngol. (Impr.) ; 84(2): 185-190, Mar.-Apr. 2018. tab
Article in English | LILACS | ID: biblio-889377

ABSTRACT

Abstract Introduction Nasal irrigation solutions are widely used following endonasal surgery. These irrigation solutions remove infective debris and crusts, reducing the probability of synechia formation, and accelerate mucosal healing. Objective The aim of the present study was to compare the effects of nasal irrigation solutions with different contents following septoplasty and concha radiofrequency. Methods The present study was a prospective, randomized, controlled simple blind study of 120 patients who underwent septoplasty and bilateral concha radiofrequency. Patients were divided into four groups according to the nasal irrigation solution used: tap water, buffered isotonic saline, saline with xylitol, and hypertonic sea water. Patients were examined on the 7th and 15th postoperative days. A saccharine test was applied to determine mucociliary activity preoperatively and on the 7th and 15th postoperative days. Patients were asked about drying and obstruction using a 10 cm visual analog scale. In addition, patients were examined to determine the crusting score. Results There was no significant difference found in the preoperative and 7th and 15th postoperative days' mucociliary clearance times among the four groups. The crusting score was found to be significantly lower in the hypertonic sea water group (p < 0.001). Drying and obstruction on the 7th and 15th postoperative days were found to be significantly more comfortable in the hypertonic sea water group (p < 0.001). Conclusion Hypertonic sea water is the recommended irrigation solution, as it is associated with less crusting, drying, and obstruction in the nose for the postoperative period following septoplasty and concha radiofrequency.


Resumo Introdução Soluções para irrigação nasal são amplamente usadas após cirurgias endonasais. Essas soluções removem os resíduos e crostas, reduzem a probabilidade de formação de sinéquias e aceleram a cicatrização da mucosa. Objetivo O objetivo do presente estudo foi comparar os efeitos das soluçoes para irrigaçao nasal com diferentes conteudos apos septoplastia e turbinoplastia com radiofrequencia. Método O presente estudo foi um estudo cego simples, randomizado, controlado e prospectivo de 120 pacientes submetidos a septoplastia e turbinoplastia bilateral com radiofrequencia. Os pacientes foram divididos em quatro grupos de acordo com a soluçao nasal utilizada: agua da torneira, soluçao salina isotonica tamponada, soluçao salina com xilitol e agua do mar hipertonica. Os pacientes foram examinados no 7° e 15° dias do pos-operatorio. O teste de sacarina foi utilizado para determinar a atividade mucociliar pre-operatoria e no 7° e 15° dias do pos-operatorio. Os pacientes foram questionados sobre a sensaçao de secura e obstruçao nasais utilizando uma escala visual analógica de 10 cm. Alem disso, os pacientes foram examinados para determinar o escore em relaçao a crostas. Resultados Não houve diferença significativa entre o pré-operatório e o sétimo e 15° dias do pós-operatório dos tempos de clearance mucociliar entre os quatro grupos. Verificou-se que o escore em relação a crostas foi significativamente menor no grupo que usou água do mar hipertônica (p < 0,001). As sensações de secura e obstrução nasais no sétimo e 15° dias do pós-operatório mostraram-se significativamente mais confortáveis no grupo água do mar hipertônica (p < 0,001). Conclusão A água de mar hipertônica é a solução de irrigação recomendada, pois está associada a menor incidência de crostas, secura e obstrução nasais no pós-operatório de cirurgia de septoplastia e das conchas nasais com radiofrequência.


Subject(s)
Humans , Male , Female , Adolescent , Adult , Middle Aged , Young Adult , Rhinoplasty/adverse effects , Mucociliary Clearance/drug effects , Nasal Lavage , Fresh Water , Nasal Mucosa/drug effects , Nasal Septum/surgery , Postoperative Complications/prevention & control , Saline Solution, Hypertonic/administration & dosage , Seawater , Administration, Intranasal , Double-Blind Method , Prospective Studies , Catheter Ablation/adverse effects , Catheter Ablation/methods , Therapeutic Irrigation
10.
Article in English | WPRIM | ID: wpr-812410

ABSTRACT

Chemical examination of an EtOAc extract of cultured Aspergillus versicolor fungus from deep-sea sediments resulted in the isolation of four xanthones, eight anthraquinones and five alkaloids, including a new xanthone, oxisterigmatocystin D (1) and a new alkaloid, aspergillusine A (13). High resolution electron impact mass spectrometry (HR-EI-MS), FT-IR spectroscopy, and NMR techniques were used to elucidate the structures of these compounds, and the absolute configuration of compound 1 was established by its NMR features and coupling constant. Furthermore, the biosynthesis pathway of these xanthones and anthraquinones were deduced, and their antioxidant activity and cytotoxicity in human cancer cell lines (HTC-8, Bel-7420, BGC-823, A549, and A2780) were evaluated. The trolox equivalent antioxidant capacity (TEAC) assay indicated most of the xanthones and anthraquinones possessing moderate antioxidant activities. The Nrf2-dependent luciferase reporter gene assay revealed that compounds 6, 7, 9, and 12 potentially activated the expression of Nrf2-regulated gene. In addition, compounds 5 and 11 showed weak cytotoxicity on A with the IC values of 25.97 and 25.60 μmol·L, respectively.


Subject(s)
Anthraquinones , Antioxidants , Chemistry , Metabolism , Pharmacology , Aspergillus , Chemistry , Genetics , Metabolism , Cell Line, Tumor , Cell Survival , Gene Expression , Humans , Magnetic Resonance Spectroscopy , Molecular Structure , NF-E2-Related Factor 2 , Genetics , Metabolism , Seawater , Microbiology , Spectroscopy, Fourier Transform Infrared , Xanthones , Chemistry , Metabolism , Pharmacology
11.
Article in English | WPRIM | ID: wpr-773620

ABSTRACT

Chemical examination of an EtOAc extract of cultured Aspergillus versicolor fungus from deep-sea sediments resulted in the isolation of four xanthones, eight anthraquinones and five alkaloids, including a new xanthone, oxisterigmatocystin D (1) and a new alkaloid, aspergillusine A (13). High resolution electron impact mass spectrometry (HR-EI-MS), FT-IR spectroscopy, and NMR techniques were used to elucidate the structures of these compounds, and the absolute configuration of compound 1 was established by its NMR features and coupling constant. Furthermore, the biosynthesis pathway of these xanthones and anthraquinones were deduced, and their antioxidant activity and cytotoxicity in human cancer cell lines (HTC-8, Bel-7420, BGC-823, A549, and A2780) were evaluated. The trolox equivalent antioxidant capacity (TEAC) assay indicated most of the xanthones and anthraquinones possessing moderate antioxidant activities. The Nrf2-dependent luciferase reporter gene assay revealed that compounds 6, 7, 9, and 12 potentially activated the expression of Nrf2-regulated gene. In addition, compounds 5 and 11 showed weak cytotoxicity on A with the IC values of 25.97 and 25.60 μmol·L, respectively.


Subject(s)
Anthraquinones , Antioxidants , Chemistry , Metabolism , Pharmacology , Aspergillus , Chemistry , Genetics , Metabolism , Cell Line, Tumor , Cell Survival , Gene Expression , Humans , Magnetic Resonance Spectroscopy , Molecular Structure , NF-E2-Related Factor 2 , Genetics , Metabolism , Seawater , Microbiology , Spectroscopy, Fourier Transform Infrared , Xanthones , Chemistry , Metabolism , Pharmacology
12.
Singapore medical journal ; : 224-227, 2018.
Article in English | WPRIM | ID: wpr-687885

ABSTRACT

We present five patients with vibrio necrotising fasciitis, a lethal and disabling disease. Two of these patients had a history of exposure to either warm seawater or raw/live seafood, three had underlying chronic liver disease, and four presented with hypotension and fever. There were three deaths and four patients required intensive care unit stays. Among the two survivors, one had high morbidity. Only one patient met the criteria of Laboratory Risk Indicator for Necrotising Fasciitis score > 6. A clinician should suspect possible vibrio necrotising fasciitis if the following are present: contact with fresh seafood/warm seawater, a known history of chronic liver disease and pain that is out of proportion to cutaneous signs. All patients must be managed via intensive care in high dependency units. We recommend a two-step surgical protocol for patient management involving an initial local debridement, followed by a second-stage radical debridement and skin grafting.


Subject(s)
Aged , Aged, 80 and over , Debridement , End Stage Liver Disease , Fasciitis, Necrotizing , Diagnosis , Microbiology , General Surgery , Female , Fever , Hepatitis B , Humans , Hypotension , Male , Middle Aged , Retrospective Studies , Risk Factors , Seafood , Seawater , Severity of Illness Index , Singapore , Skin Transplantation , Vibrio , Vibrio Infections , Diagnosis , General Surgery
13.
Article in English | WPRIM | ID: wpr-718189

ABSTRACT

Vibrio vulnificus is a gram-negative bacterium that can cause serious, potentially fatal infections. V. vulnificus causes three distinct syndromes: an overwhelming primary septicemia caused by consuming contaminated seafood, wound infections acquired when an open wound is exposed to contaminated warm seawater, and gastrointestinal tract-limited infections. Case-fatality rates are higher than 50% for primary septicemia, and death typically occurs within 72 hours of hospitalization. Risk factors for V. vulnificus infection include chronic liver disease, alcoholism, and hematological disorders. When V. vulnificus infection is suspected, appropriate antibiotic treatment and surgical interventions should be performed immediately. Third-generation cephalosporin with doxycycline, or quinolone with or without third-generation cephalosporin, may be potential treatment options for patients with V. vulnificus infection.


Subject(s)
Alcoholism , Diagnosis , Doxycycline , Hospitalization , Humans , Liver Diseases , Prognosis , Public Health , Risk Factors , Seafood , Seawater , Sepsis , Vibrio vulnificus , Vibrio , Wound Infection , Wounds and Injuries
14.
Article in Korean | WPRIM | ID: wpr-716738

ABSTRACT

In order to investigate the occurrence of norovirus in rivers and beaches, a total of 81 samples were tested at seven sites of Oncheon stream, Suyeong river and Gwanganri beach in Busan from January to November, 2017. To improve the detection of norovirus from sea water, we applied the inorganic cation-coated filter method which showed 48.8% ± 12.2% (n=3) and 27.4% ± 6.0% (n=3) recovery yields from river water and sea water inoculated with Norovirus, respectively. Norovirus was detected in a total of four samples (4.9%), which all were GII genotype. Norovirus GII was detected in three samples at two waste water treatment plants (WWTP) outlet and one sample at about 500 meter downstream from WWTP in both the winter and spring seasons. We also monitored fecal indicator organisms, Escherichia coli (E. coli), Enterococcus and coliphages [somatic coliphages (SC), male-specific coliphages (MSC)] to analyze the potential transmission of enteritis causative agent in dry and wet days. Bacterial influences were found at the site of the WWTP effluents in the dry days and spread further to the costal beach in the wet days. But no viral influences were found in the river downstream in both dry and wet days.


Subject(s)
Coliphages , Enteritis , Enterococcus , Escherichia coli , Genotype , Korea , Methods , Norovirus , Rivers , Seasons , Seawater , Waste Water , Water
15.
Braz. j. microbiol ; 48(4): 671-679, Oct.-Dec. 2017. tab, graf
Article in English | LILACS | ID: biblio-889179

ABSTRACT

ABSTRACT Thraustochytrids are unicellular protists belonging to the Labyrinthulomycetes class, which are characterized by the presence of a high lipid content that could replace conventional fatty acids. They show a wide geographic distribution, however their diversity in the Antarctic Region is rather scarce. The analysis based on the complete sequence of 18S rRNA gene showed that strain 34-2 belongs to the species Thraustochytrium kinnei, with 99% identity. The total lipid profile shows a wide range of saturated fatty acids with abundance of palmitic acid (16:0), showing a range of 16.1-19.7%. On the other hand, long-chain polyunsaturated fatty acids, mainly docosahexaenoic acid and eicosapentaenoic acid are present in a range of 24-48% and 6.1-9.3%, respectively. All factors analyzed in cells (biomass, carbon consumption and lipid content) changed with variations of culture temperature (10 °C and 25 °C). The growth in glucose at a temperature of 10 °C presented the most favorable conditions to produce omega-3fatty acid. This research provides the identification and characterization of a Thraustochytrids strain, with a total lipid content that presents potential applications in the production of nutritional supplements and as well biofuels.


Subject(s)
Seawater/microbiology , Fatty Acids/metabolism , Stramenopiles/metabolism , Phylogeny , Biotechnology , Fatty Acids/chemistry , Stramenopiles/isolation & purification , Stramenopiles/classification , Stramenopiles/genetics , Antarctic Regions
16.
Electron. j. biotechnol ; 29: 13-21, sept. 2017. ilus, tab, graf
Article in English | LILACS | ID: biblio-1017057

ABSTRACT

Background: The past years have witnessed a growing number of researches in biofilm forming communities due to their environmental and maritime industrial implications. To gain a better understanding of the early bacterial biofilm community, microfiber nets were used as artificial substrates and incubated for a period of 24 h in Mauritian coastal waters. Next-generation sequencing technologies were employed as a tool for identification of early bacterial communities. Different genes associated with quorum sensing and cell motility were further investigated. Results: Proteobacteria were identified as the predominant bacterial microorganisms in the biofilm within the 24 h incubation, of which members affiliated to Gammaproteobacteria, Alphaproteobacteria and Betaproteobacteria were among the most abundant classes. The biofilm community patterns were also driven by phyla such as Firmicutes, Bacteroidetes, Chloroflexi, Actinobacteria and Verrucomicrobia. The functional analysis based on KEGG classification indicated high activities in carbohydrate, lipid and amino acids metabolism. Different genes encoding for luxI, lasI, agrC, flhA, cheA and cheB showed the involvement of microbial members in quorum sensing and cell motility. Conclusion: This study provides both an insight on the early bacterial biofilm forming community and the genes involved in quorum sensing and bacterial cell motility.


Subject(s)
Seawater/microbiology , Bacteria/growth & development , Bacteria/genetics , Bacterial Physiological Phenomena , Bacteria/isolation & purification , Bacteria/classification , Bacterial Adhesion , Cell Movement , Biofilms , Biodiversity , Quorum Sensing , Biofouling , Metagenomics , High-Throughput Nucleotide Sequencing , Mauritius
17.
Braz. j. microbiol ; 48(3): 393-394, July-Sept. 2017.
Article in English | LILACS | ID: biblio-889136

ABSTRACT

Abstract Dietzia sp. 111N12-1, isolated from the seawater of South China Sea, shows strong petroleum hydrocarbons degradation activity. Here, we report the draft sequence of approximately 3.7-Mbp genome of this strain. To the best of our knowledge, this is the first genome sequence of Dietzia strain isolated from the sea. The genome sequence may provide fundamental molecular information on elucidating the metabolic pathway of hydrocarbons degradation in this strain.


Subject(s)
Seawater/microbiology , Actinomycetales/isolation & purification , Actinomycetales/genetics , Genome, Bacterial , Hydrocarbons/metabolism , Phylogeny , Biodegradation, Environmental , Actinomycetales/classification , Actinomycetales/metabolism , Petroleum/metabolism , Base Sequence , China
18.
19.
Ciênc. saúde coletiva ; 22(6): 2037-2048, jun. 2017. tab, graf
Article in Portuguese | LILACS | ID: biblio-840011

ABSTRACT

Resumo Emissários submarinos são apresentados como uma eficiente alternativa para o destino final de efluentes sanitários em regiões costeiras densamente povoadas em virtude da elevada capacidade de dispersão e depuração da matéria orgânica no ambiente marinho, e por demandar pequenas áreas para sua implementação. A probabilidade de condições improprias de balneabilidade, em áreas costeiras adjacentes aos emissários submarinos de esgotos de Ipanema, Barra da Tijuca e Icaraí, é avaliada com base em metodologia computacional probabilística que contempla em conjunto a modelagem hidrodinâmica, de transporte e decaimento bacteriano. Os resultados mostram que as concentrações de coliformes fecais são influenciadas fortemente pela radiação solar e todos os fatores responsáveis por sua mitigação no ambiente marinho. Os referidos emissários não comprometem a balneabilidade na região costeira, visto que as condições improprias de balneabilidade são restringidas às regiões adjacentes aos pontos de lançamento do efluente. A poluição observada nas praias é indicativa da contaminação que ocorre pelos sistemas lagunares, rios e canais ambientalmente degradados.


Abstract Submarine outfalls are proposed as an efficient alternative for the final destination of wastewater in densely populated coastal areas, due to the high dispersal capacity and the clearance of organic matter in the marine environment, and because they require small areas for implementation. This paper evaluates the probability of unsuitable bathing conditions in coastal areas nearby to the Ipanema, Barra da Tijuca and Icaraí outfalls based on a computational methodology gathering hydrodynamic, pollutant transport, and bacterial decay modelling. The results show a strong influence of solar radiation and all factors that mitigate its levels in the marine environment on coliform concentration. The aforementioned outfalls do not pollute the coastal areas, and unsuitable bathing conditions are restricted to nearby effluent launching points. The pollution observed at the beaches indicates that the contamination occurs due to the polluted estuarine systems, rivers and canals that flow to the coast.


Subject(s)
Humans , Environmental Monitoring/methods , Waste Water/analysis , Water Pollutants/analysis , Water Pollution/analysis , Brazil , Environmental Health , Risk , Rivers , Seawater/analysis , Sewage/analysis , Ships
20.
Rev. biol. trop ; 65(1): 375-392, Jan.-Mar. 2017. ilus
Article in English | LILACS | ID: biblio-897549

ABSTRACT

Abstract:Previous work has highlighted the critical role of macroalgal productivity and dynamics in supporting and structuring marine food webs. Spatio-temporal variability in macroalgae can alter coastal ecosystems, a relationship particularly visible along upwelling-influenced coastlines. As a result of its equatorial location and nutrient rich, upwelling-influenced waters, the Galápagos Archipelago in the East Pacific, hosts a productive and biodiverse marine ecosystem. Reports and collections of macroalgae date back to the Beagle voyage, and since then, more than three hundred species have been reported. However, their ecology and functional role in the ecosystem is not well understood. According to various disparate and in part anecdotal sources of information, abundant and diverse communities exist in the Western regions of the archipelago, the North is essentially barren, and in the central/South abundance and distribution is variable and less well defined. Both oceanographic conditions and herbivore influence have been theorized to cause this pattern. Extensive changes in macroalgal productivity and community composition have occurred during strong ENSO events, and subsequent declines in marine iguana (an endemic and iconic grazer) populations have been linked to these changes. Iguanas are only one species of a diverse and abundant group of marine grazers in the system, highlighting the potentially important role of macroalgal productivity in the marine food web. This review represents a first compilation and discussion of the available literature and presents topics for future research. Rev. Biol. Trop. 65 (1): 375-392. Epub 2017 March 01.


ResumenTrabajos previos han destacado el papel fundamental de la productividad y dinámica de las macroalgas en el mantenimiento y estructuración de las cadenas alimentarias marinas. La variabilidad espacio temporal de las macroalgas puede alterar ecosistemas costeros, particularmente visibles a lo largo del perfil costero en zonas de proliferación. Como resultado de su ubicación ecuatorial y riqueza en nutrientes, además de eventos de proliferación, el Archipiélago de Galápagos en el Pacífico Oriental acoge un productivo y biodiverso ecosistema marino. Informes y recolección de macroalgas se remontan desde el viaje del Beagle, y desde entonces se han informado más de trescientas especies. No obstante, su función ecológica en el ecosistema no ha sido bien comprendida. Según diversas fuentes de información y en parte anecdóticas, existen abundantes y diversas comunidades de macroalgas en las regiones occidentales del archipiélago, el norte es sustancialmente estéril, y en el centro/sur la abundancia y distribución es variable y menos definida. Tanto las condiciones oceanográficas y la influencia de herbívoros han sido teorizadas para causar este patrón. Grandes cambios en la composición de la productividad y comunidad de macroalgas se han producido durante eventos ENOS fuertes y subsecuentemente han provocado la disminución de poblaciones de iguana marina (herbívoro endémico e icónico) y han sido vinculados a estos cambios. Las iguanas marinas son sólo una de las especies de un grupo diverso y abundante de herbívoros marinos en el sistema, destacando potencialmente el importante rol de la productividad de macroalgas en la cadena alimentaria marina. Esta revisión representa una primera recopilación y análisis de la literatura disponible y presenta temas para futuras investigaciones.


Subject(s)
Animals , Seaweed/physiology , Biodiversity , Seawater , Species Specificity , Time Factors , Food Chain , Ecuador , Herbivory/physiology
SELECTION OF CITATIONS
SEARCH DETAIL