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Article in Chinese | WPRIM | ID: wpr-921706


This study adopted headspace-gas chromatography-mass spectrometry(HS-GC-MS) and electronic nose to detect volatile components from Myristicae Semen samples with varying degrees of mildew, aiming at rapidly identifying odor changes and substance basis of Myristicae Semen mildew. The experimental data were analyzed by electronic nose and principal component analysis(PCA). The results showed that Myristicae Semen samples were divided into the following three categories by electronic nose and PCA: mildew-free samples, slightly mildewy samples, and mildewy samples. Myristicae Semen samples with different degrees of mildew greatly varied in volatile components. The volatile components in the samples were qualitatively and quantitatively detected by HS-GC-MS, and 59 compounds were obtained. There were significant differences in the composition and content in Myristicae Semen samples with different degrees of mildew. The PCA results were the same as those by electronic nose. Among them, 3-crene, D-limonene, and other terpenes were important indicators for the identification of mildew. Bicyclo[3.1.0]hexane, 4-methylene-1-(1-methylethyl)-, terpinen-4-ol, and other alcohols were key substances to distinguish the degree of mildew. In the later stage of mildew, Myristicae Semen produced a small amount of hydroxyl and aldehyde compounds such as acetaldehyde, 2-methyl-propionaldehyde, 2-methyl-butyraldehyde, and formic acid, which were deduced as the material basis of the mildew. The results are expected to provide a basis for the rapid identification of Myristicae Semen with different degrees of mildew, odor changes, and the substance basis of mildew.

Electronic Nose , Gas Chromatography-Mass Spectrometry , Odorants/analysis , Semen/chemistry , Solid Phase Microextraction , Volatile Organic Compounds/analysis
Article in Chinese | WPRIM | ID: wpr-879169


Based on the systematic retrieval and the reported components of Sojae Semen Nigrum and Sojae Semen Praeparatum, this study conducted in-depth analysis of conversion of components in the fermentation process, and discussed types and possible mec-hanisms of conversion of chemical components, so as to provide the basis for studying technology, medicinal ingredients and quality standards. According to the analysis, there is a certain degree of conversion of nutrients(like protein, sugar, lipid), bioactive substances(like isoflavones, saponins, γ-aminobutyric acid) and other substances(like nucleosides, melanoids, biamines, etc) in the process of fermentation.

Chromatography, High Pressure Liquid , Fermentation , Isoflavones/analysis , Semen/chemistry , Soybeans
Article in Chinese | WPRIM | ID: wpr-878913


In the process of harvesting, production and processing, storage, and transportation, the traditional Chinese medicine Platycladi Semen is prone to mildew due to its own and environmental factors, which can nourish the production of toxic or pathogenic fungi, and even produce mycotoxins, which affects the safety of clinical medication. The 2020 edition of Chinese Pharmacopoeia limits the highest standard of aflatoxin content in Platycladi Semen. However, there are few studies on the fungal contamination of Platycladi Semen, and it is difficult to prevent and control it in a targeted manner. Therefore, based on the Illumina NovaSeq6000 platform, this article uses ITS sequence amplicon technology to analyze the distribution and diversity of fungi in 27 batches of commercially available Platycladi Semen in the Chinese market. A total of 10 phyla, 35 classes, 93 orders, 193 families, 336 genera, and 372 species of fungi were identified in China. Among them, Aspergillus, Alternaria spp. were dominant, 20 batches of samples were detected for A. flavus, 10 batches of samples were detected for A. nidulans, and all samples were detected for potential pathogenic fungi such as A. fumigatus and A. niger. According to diversity analysis, the diversity of the fungal communities in the samples from Gansu province was high, the samples in Shandong province contain the largest number of fungal species, and the samples in Guangxi province had the lo-west diversity and the least number of species. In most samples, pathogenic fungi such as A. fumigatus, A. niger, A. flavus, A. parasiticus were detected in varying degrees. This study systematically investigated the fungal contamination of Platycladi Semen from the markets in the last link of the its industrial chain, and clarified the distribution of Platycladi Semen fungi, especially toxin-producing fungi, and provided theoretical basis for the targeted prevention and control of fungal contamination in Platycladi Semen.

Aflatoxins , China , Fungi/genetics , Humans , Mycobiome , Mycotoxins/analysis , Semen/chemistry
Arq. bras. med. vet. zootec. (Online) ; 72(5): 1727-1730, Sept.-Oct. 2020. tab
Article in English | LILACS, VETINDEX | ID: biblio-1131576


There is a need for various anesthetic agents to obtain sperm in the field of human and veterinary medicine. Propofol and midazolam are among the most preferred among these agents. The aim of this study was to determine how sperm paramaters are affected according to the anesthetic agent used. Propofol (2mg/kg) and midazolam (3,5-7,5mg/kg) were administered twice a day (morning-evening) for one week. As a result of this study, there was no statistical difference in sperm density and abnormal sperm rates (respectively P=0,673, P=0,479). Sperm motility rates are similar in the control and propofol groups, while the motility rate in the midazolam group is statistically lower. (Control group %85 - Midazolam group %68.75 - Propofol group %83.75), (P<0.05). As a result of this study, the confidence interval of propofol was higher than the other anesthetic agents used for sperm retrieval.(AU)

São necessários vários agentes anestésicos para obter espermatozoides no campo da medicina humana e veterinária. Propofol e midazolam estão entre os agentes preferidos. O objetivo deste estudo foi determinar como os parâmetros de esperma são afetados de acordo com o agente anestésico utilizado. Propofol (2mg / kg) e midazolam (3,5-7,5mg / kg) foram administrados duas vezes ao dia (manhã e noite) durante uma semana. Neste estudo, não houve diferença estatística na densidade espermática e nas taxas anormais de espermatozoides (respectivamente P = 0,673, P = 0,479). As taxas de motilidade espermática são semelhantes nos grupos controle e propofol, enquanto a taxa de motilidade no grupo midazolam é estatisticamente menor. (Grupo controle % 85 - grupo midazolam % 68,75 - grupo propofol % 83,75), (P <0,05). Neste estudo, o intervalo de confiança do propofol foi maior do que os outros agentes anestésicos utilizados na recuperação espermática.(AU)

Animals , Male , Rats , Semen/chemistry , Spermatozoa/physiology , Midazolam/administration & dosage , Propofol/administration & dosage
Int. braz. j. urol ; 45(6): 1113-1121, Nov.-Dec. 2019. tab, graf
Article in English | LILACS | ID: biblio-1056346


ABSTRACT Purpose: To establish whether the citrate concentration in the seminal fluid ([CITRATE]) measured by means of high-resolution nuclear magnetic resonance spectroscopy (1HNMRS) is superior to the serum prostate-specific antigen (PSA) concentration in detecting of clinically significant prostate cancer (csPCa) in men with persistently elevated PSA. Materials and Methods: The group of patients consisted of 31 consecutively seen men with histological diagnosis of clinically localized csPCa. The control group consisted of 28 men under long-term follow-up (mean of 8.7 ± 3.0 years) for benign prostate hyperplasia (BPH), with persistently elevated PSA (above 4 ng/mL) and several prostate biopsies negative for cancer (mean of 2.7 ± 1.3 biopsies per control). Samples of blood and seminal fluid (by masturbation) for measurement of PSA and citrate concentration, respectively, were collected from patients and controls. Citrate concentration in the seminal fluid ([CITRATE]) was determined by means of 1HNMRS. The capacities of PSA and [CITRATE] to predict csPCa were compared by means of univariate analysis and receiver operating characteristic (ROC) curves. Results: Median [CITRATE] was significantly lower among patients with csPCa compared to controls (3.93 mM/l vs. 15.53 mM/l). There was no significant difference in mean PSA between patients and controls (9.42 ng/mL vs. 8.57 ng/mL). The accuracy of [CITRATE] for detecting csPCa was significantly superior compared to PSA (74.8% vs. 54.8%). Conclusion: Measurement of [CITRATE] by means of 1HNMRS is superior to PSA for early detection of csPCa in men with elevated PSA.

Humans , Male , Aged , Prostatic Neoplasms/diagnosis , Semen/chemistry , Prostate-Specific Antigen/blood , Citric Acid/analysis , Prostatic Hyperplasia/diagnosis , Prostatic Hyperplasia/blood , Prostatic Neoplasms/pathology , Prostatic Neoplasms/blood , Biopsy , Biomarkers, Tumor/analysis , Reproducibility of Results , Sensitivity and Specificity , Statistics, Nonparametric , Risk Assessment , Middle Aged
Int. j. morphol ; 35(1): 99-104, Mar. 2017. ilus
Article in English | LILACS | ID: biblio-840939


Our aim was to describe sperm parameters in residents from Northern Chile. We evaluated in 101 volunteers (18 and 30 years old) urinary and drinking water Boron levels using Inductively Coupled Plasma Mass Spectrometry; semen parameters were measured with standardized methods. Each individual was categorized in 3 levels of exposure: low (B levels in urine 2.94 mgL-1 or tap water 3.0 mgL-1), medium (urinary B between 2.95-7.4 mgL-1 and B in tap water with 3.0-7.0 mgL-1) and high (urinary B > 7.4 mgL-1 or tap water > 7.0 mgL-1). We found no significant differences among groups by pH, sperm concentration (45.1; 48.2 and 38 million/mL), motility 1th hour (38.1; 40.0 and 45.5 %) and vitality 1th hour (88.6; 88.0 and 76.9 %) respectively. Abnormal morphology was significant different (83.3; 90 and 83 %). Young men exposed to B in drinking water present sperm variations associated with the level of exposure. Most of these changes are positive at intermediate levels of B. For the highest exposures were observed negative changes in sperm morphology, concentration, motility and vitality, all relevant parameters of fertility. Beneficial effect is observed at medium exposure, like a "U curve".

El objetivo de este trabajo fue describir los parámetros espermáticos en residentes del norte de Chile. Se evaluaron en 101 voluntarios (18 y 30 años), los niveles urinarios y de agua potable de boro, usando "Inductively Coupled Plasma Mass Spectrometry". Los parámetros del semen se midieron con métodos estandarizados. Cada individuo se clasificó en 3 niveles de exposición: bajo (niveles B en la orina 2,94 mgL-1 o agua potable 3,0 mgL-1), medio (B urinario entre 2,95-7,4 mgL-1 y B en agua de beber con 3,0- 7,0 mgL-1) y alto (B urinario >7,4 mgL-1 o agua potable > 7,0 mgL-1). No se encontraron diferencias significativas entre los grupos por pH, concentración de espermatozoides (45,1; 48,2 y 38 millones/mL), motilidad a 1 hora (38,1; 40,0 y 45,5%) y vitalidad 1 hora (88,6; 88,0 y 76,9%) respectivamente. La morfología anormal fue significativamente diferente (83,3; 90 y 83%). Los hombres jóvenes expuestos a B en el agua potable presentan variaciones espermáticas asociadas con el nivel de exposición. La mayoría de estos cambios son positivos en niveles intermedios de B. Para las exposiciones más altas se observaron cambios negativos en la morfología, concentración, motilidad y vitalidad del esperma, parámetros relevantes de la fertilidad. Un efecto beneficioso se observa en la exposición media, como una "curva U".

Humans , Male , Female , Adolescent , Adult , Boron/toxicity , Spermatozoa/drug effects , Water Pollutants, Chemical/toxicity , Boron/urine , Chemical Compound Exposure , Chile , Fertility/drug effects , Mass Spectrometry/methods , Semen/chemistry , Semen/drug effects , Spermatozoa/pathology , Water Pollutants, Chemical/urine
Arq. ciênc. vet. zool. UNIPAR ; 17(4): 227-231, out.-dez.2014. tab, graf
Article in English | LILACS | ID: lil-758592


This experiment was carried out with the objective of evaluating different levels of powder propolis in rabbit diets and their effect on semen characteristics. A total of 36 New Zealand White male rabbits were used, randomly distributed into six groups, corresponding to six propolis levels (0, 0.25, 0.50, 0.75, 1.0 and 1.25 g propolis/kg of ration). Semen was collected twice a week, using an artificial vagina. Semen volume, progressive spermatic motility, spermatic vigor, spermatic concentration and spermatic morphology were analyzed. General linear models were used for statistical analysis. The inclusion of powder propolis in the diet increased normal spermatozoa percentage and reduced spermatozoa abnormalities. The powder propolis did not affect the progressive spermatic motility, spermatic vigor or spermatic concentration. The values were considered normal for rabbits. However, a small reduction in semen volume was observed, without any negative effect on the other semen characteristics evaluated. Thus, it is possible to observe better semen quality with the inclusion of 1.25 g powder propolis/kg in the diet for reproducer rabbits...

Este experimento foi realizado com o objetivo de avaliar a influência de diferentes níveis de própolis em pó na ração de coelhos sobre as características do sêmen. Utilizaram-se 36 coelhos machos, adultos, Nova Zelândia Brancos, divididos aleatoriamente em seis grupos, consumindo cinco níveis de própolis (0; 0,25; 0,50; 0,75; 1,0 e 1,25 g de própolis/kg de ração). Coletou-se sêmen duas vezes por semana, utilizando vagina artificial. Verificou-se o volume, a motilidade espermática progressiva, o vigor espermático, a concentração espermática e a morfologia espermática. As análises estatísticas foram realizadas utilizando os modelos lineares generalizados. A adição da própolis na ração elevou a porcentagem de espermatozóides normais e reduziu os anormais. Todavia, foi observada uma pequena redução no volume do sêmen com o aumento do nível de própolis na dieta, sem afetar as demais características do sêmen. A motilidade progressiva, vigor espermático e concentração espermática não foram influenciados pelos diferentes níveis de própolis, valores considerados normais para coelhos. Conclui-se que a melhor qualidade do sêmen de coelhos reprodutores ocorreu com a adição de 1,25 g de própolis/kg de ração...

Este experimento se llevó a cabo para evaluar la influencia de diferentes niveles de polvo de propóleos en la dieta de conejos, bajo las características del semen. Se utilizó 36 conejos machos, adultos, Nueva Zelanda Blancos, divididos al azar en seis grupos, consumiendo cinco niveles de propóleos (0; 0,25; 0,50; 0,75; 1,0 y 1,25g de propóleos/kg en el alimento). Se recogió semen dos veces a la semana, utilizando vagina artificial. Se encontró el volumen, motilidad espermática progresiva, el vigor de espermático, la concentración espermática y la morfología espermática. Los análisis estadísticos se realizaron utilizando modelos lineales generales. La adición de propóleos en la dieta aumentó el porcentaje de espermatozoides normales y redujo los anormales. Sin embargo, se ha observado una pequeña reducción en el volumen del semen con el aumento de propóleos en la dieta, sin afectar las demás características del semen. La motilidad progresiva, vigor espermático y concentración de espermatozoides no se vieron afectados por los diferentes niveles de propóleos, valores considerados normales para conejos. Se concluye que la mejor calidad del semen de conejos reproductores ocurrió con la adición de 1,25g de propóleos / kg en el alimento...

Animals , Male , Propolis/administration & dosage , Propolis/metabolism , Propolis , Animal Feed/analysis , Animal Feed , Semen/chemistry
Arq. bras. med. vet. zootec ; 65(1): 6-12, fev. 2013. tab
Article in English | LILACS | ID: lil-667529


The present study aimed to verify the caprine semen characteristics during dry and rainy seasons in the Brazilian Northeast, and the influence of these seasons on cooled semen. Seminal volume, concentration, percentage of motile cells, vigor and spermatic morphology, as well as biochemical profile (fructose, citric acid, P, Ca2+, Mg, total proteins and phospholipase A2 activity) were analyzed. It was observed a reduction (P<0.05) in normal sperm morphology, fructose, citric acid, P, Mg and total protein concentration during the dry season, which did not affect the motility, vigor, volume and sperm concentration. Phospholipase A2 activity was increased during the dry season (P<0.05). The analysis of the semen cooled at 4ºC during 48 hours showed reduction in total motility and vigor sperm during the dry season (P<0.05). Based on these results, we conclude that the best period of year for caprine semen cooling is the rainy season.

Verificou-se as características seminais de caprinos durante a época seca e a chuvosa no Nordeste brasileiro e a influência da época no resfriamento do sêmen. Foram mensurados volume, concentração espermática, porcentagem de espermatozoides móveis, vigor, morfologia espermática e características bioquímicas (frutose, ácido cítrico, fósforo, magnésio, proteínas totais e atividade da fosfolipase A2). Observou-se redução (P<0,05) no número de espermatozóides morfologicamente normais, frutose, ácido cítrico, fósforo, magnésio e proteínas totais durante a época seca que não influenciaram na motilidade, vigor, volume e concentração do sêmen. Entretanto, a atividade da fosfolipase A2 foi maior na época seca. Quando o sêmen foi submetido ao resfriamento a 4ºC durante 48 horas, houve redução (P<0,05) na motilidade total e no vigor espermático durante a época seca. Com base nesses resultados, conclui-se que o período chuvoso é melhor para resfriar sêmen de caprinos no Nordeste brasileiro.

Animals , Male , Semen Preservation/statistics & numerical data , Semen Preservation/veterinary , Semen/chemistry
Int. braz. j. urol ; 37(4): 495-506, July-Aug. 2011. tab
Article in English | LILACS | ID: lil-600815


OBJECTIVES: (i) To examine the role of apoptosis in the pathogenesis of DNA damage in semen from infertile men. (ii) To assess the effects of smoking on apoptotic markers and seminal parameters among infertile men. (iii) To assess the correlation of apoptosis with conventional semen parameters. MATERIALS AND METHODS: The study was carried out on 70 men with idiopathic infertility, divided into two groups: thirty infertile non smokers and forty infertile smokers. In addition to 60 fertile men (30 non smokers and 30 smokers) as control group. Each subject provided semen for analysis of parameters, determination of percent of DNA fragmentation, s-Fas, caspase-3 activity levels and cotinine levels. RESULTS: The results revealed that infertile men, particularly smokers have significantly lower semen variables and significantly higher levels of apoptotic variables ( percent of DNA fragmentation, s-Fas and caspase-3 activity) in addition to cotinine. CONCLUSIONS: The present findings provide additional evidence supporting the importance of the evaluation of apoptotic markers to test male infertility particularly among smokers.

Adult , Humans , Male , Apoptosis/physiology , DNA Fragmentation , Infertility, Male/genetics , Semen/chemistry , Smoking/adverse effects , Analysis of Variance , Biomarkers/analysis , Case-Control Studies , Sperm Motility , Statistics, Nonparametric , Time Factors
Zanco Journal of Medical Sciences. 2011; 14 (2): 34-39
in English | IMEMR | ID: emr-122792


Male Infertility is often caused by problems with sperm production or motility. Zinc in human semen seems to play an important role in the physiology of spermatozoa This study was designed to demonstrate the relationships between concentrations of zinc and testosterone in serum and seminal plasma and sperm quality among infertile men. One hundred four infertile males, aged [19-44] years, were selected from Infertile Clinic-Azadi Teaching Hospital- Kirkuk Province. Forty known fertile males were selected as normospermic control group. Semen samples were analyzed according to WHO criteria. Serum and seminal plasma zinc concentrations were estimated by atomic absorption technique. Serum testosterone was measured by MiniVIDAS apparatus. The mean value of serum testosterone was significantly lower in infertile males [4.87 +/- 0.15 ng/ml] as compared to control group [6.41 +/- 0.16 ng/ml]; [P< 0.01], significant correlations were observed between serum testosterone with seminal plasma zinc level in oligospermic subjects [r=0.44] and with serum zinc level in azoospermic subjects [r=0.37], [P< 0.01]; [P< 0.05] respectively. Serum and seminal plasma zinc levels were lower in infertile men [7.75 +/- 0.18 micromol/L]; [0.83 +/- 0.02 mmol/L] when compared with normospermic control group [14.09 +/- 0.27 Mmol/L]; [1.41 +/- 0.01 mmol/L] respectively [p<0.01], Zinc may contribute to fertility through its positive effect on spermatogenesis. Also there was significant decrease in serum and seminal plasma zinc levels in oligospermic and azoospermic infertile males with significantly low androgen. It indicates that the zinc may have a role for steroidogenesis

Humans , Male , Young Adult , Adult , Infertility, Male/blood , /blood , Zinc/blood , Zinc/analysis , Semen/chemistry , Spermatogenesis
Veterinary Medical Journal. 2010; 58 (2): 137-150
in English | IMEMR | ID: emr-117304


The goal of the present study is to evaluate the effect of feed restriction on chromosomal pictures, serum biochemical parameters and some hormones in addition to spermiogram with their reflection on the reproductive efficiency of rams. Five mature Crossbred [Barki x Rahmani] rams were used, their age ranged from 15 to 17 months and weighting 45 to 60 kg. Rams received a basal diet according to the management of Animal Reproduction Research Institute [ARRI]. The animals were exposed to 21 days of feed restriction [1/3 amount of basal diet]. Restricted rams refed again on basal diet for 14 days. Blood and semen samples were collected from rams [before feed restriction, 21 days after feed restriction and 14 days post refeeding]. The results showed that there were significant differences between first period and others in the percentage of some chromosomal abnormalities which mainly were polydiploidy, in addition to gaps, breaks, deletions and fragments which increased significantly only after feed restricted periods in addition to the significant increase in the percent of total number of aberrated cells. Concerning the biochemical parameters, there was a significant decrease in serum glucose and cholesterol levels after feed restriction and they were elevated after refeeding to their levels before the restriction. Malondialdehyde showed a significant decrease after refeeding than the other two periods of the experiment. On the contrary, there was a significant increase in serum insulin levels after feed restriction compared with the other two periods. The effect of feed restriction was clear on serum testosterone hormone level which showed significant decrease after feed restriction and after refeeding. There was significant decrease in semen volume after restriction and refeeding rams. There was significant decrease in the motility and live/dead sperm after feed restriction and return to their levels post refeeding. Concentration of semen showed significant decrease after both feed restriction and refeeding. Total sperm abnormalities showed significant increase after restriction and back to first level before restriction. It was concluded that, feed restriction [l/3 amount of basal ration] for 21 days in mature rams has adverse effect on the chromosomal pictures, some biochemical parameters and decreased testosterone level in addition to decrease in all semen parameters. This effect was not overcome by supplemental feeding again that confirming the status of these rams did not improve completely even after the tested refeeding period

Animals , Semen Analysis/methods , Semen/chemistry , Food Supply/methods , Chromosome Disorders , Cytogenetic Analysis/methods
Pakistan Journal of Physiology. 2010; 6 (1): 36-38
in English | IMEMR | ID: emr-123394


A great number of substances have been found in sperm plasma but so far it has not been possible to provide evidence of clinical significance for all of them. Fructose occupies the most important place on biochemical investigations. Fructose acts as a donor of energy to the spermatozoa. Fructose is secreted from the seminal vesicles and the accessory sex glands. It is the major carbohydrate found in seminal plasma, and appears essential for normal sperm motility. We present results of a prospective study of seminal fructose in patients referred for routine semen analysis prior to infertility treatment. Qualitative measurement of fructose in seminal fluid was carried out by Resorcinol method. Fructose level in various groups of male infertility, and sperm concentration in various groups was estimated. They were classified as azoospermic, oligozoospermic, polyzoospermic, normozoospermic, asthenozoospermic, and teratozoospermic on the basis of sperm concentrations, motility and morphology respectively. It is indicated that the true corrected fructose level is a simple method for assessment of the seminal vesicular function

Humans , Male , Seminal Vesicles , Fructose/analysis , Prospective Studies , Semen/chemistry , Sperm Motility
Rev. cient. (Maracaibo) ; 19(5): 500-505, sept.-oct. 2009. tab
Article in Spanish | LILACS | ID: lil-551221


El objetivo del presente estudio fue evaluar la integridad acrosomal, funcionalidad bioquímica de la membrana espermática y las relaciones entre éstas en machos con y sin persistencia de gotas citoplásmicas (PGC). Se usaron 254 eyaculados de 48 cerdos reproductores entre ocho y 48 meses de edad clasificados como jóvenes (<1 año), maduros (entre uno y tres años) y viejos (>3 años). Los datos fueron evaluados mediante análisis de varianza para medidas repetidas en un mismo individuo (efecto aleatorio). Se evaluó el efecto de procedencia (efecto bloque) y grupo etáreo sobre: PGC, integridad acrosomal, funcionalidad de membrana, proporción de espermatozoides vivos y con gotas citoplásmicas (GC). Además se evaluó el efecto de PGC sobre la integridad acrosomal y la funcionalidad de membrana. La presentación de PGC fue menor en cerdos reproductores jóvenes (37 ± 6 por ciento) que en los maduros (52 ± 5 por ciento; P=0,05) y los viejos (85 ± 13 por ciento; P<0,01). La diferencia en PGC entre machos maduros y viejos fue significativa (P=0,05). Los machos viejos presentaron mayor proporción de células con GC que los jóvenes (34,6 ± 4,8 vs. 19,7 ± 2.3 por ciento, respectivamente; P=0,01), mientras que los maduros no fueron diferentes de ninguno de los dos (23,5 ± 2,1 por ciento). Los cerdos reproductores que no presentaron PGC obtuvieron mayores valores de acrosoma intacto que los que presentaron PGC (86,5 ± 1,2 vs. 80,2 ± 1,1 por ciento, respectivamente; P<0,01). Los defectos de la integridad acrosomal presentaron correlaciones significativas con defectos espermáticos asociados a GC (entre -0,20 y -0,36; P<0,05). Este estudio indica que cerdos reproductores mayores de tres años presentan con mayor frecuencia eyaculados de baja calidad espermática. Los resultados además sugieren que los defectos de membrana y morfológicos tienden a presentarse conjuntamente en un eyaculado, señalando la necesidad de construir un índice de calidad seminal multifactorial...

The goal of this study was to evaluate the acrosomal integrity, biochemical functionality of the sperm membrane, and the relationship between them in boars with and without persistence of cytoplasmic droplets (PGC). It were used 254 ejaculates from 48 boars between eight and 48 months of age that were classified as young (<1 year), mature (1 to 3 years), and old (>3 years). Data were analyzed using analysis of variance for repeated measurements in the same individual (random effect). The effect of source (block effect) and age group on PGC, acrosomal integrity, membrane function, and proportion of live and with cytoplasmic droplets sperm cells were evaluated. The effect of PGC on acrosomal integrity and membrane function was also tested. Presence of PGC was lower in young (37 ± 6 percent) than mature (52 ± 5 percent; P=0.05) and old (85 ± 13 percent; P<0.01) boars. The difference in PGC between mature and old boars was significant (P=0.05). Old boars showed a higher proportion of cells with GC than young ones (34.6 ± 4.8 vs. 19.7 ± 2.3 percent, respectively; P=0.01), while mature boars were no different from the other two groups (23.5 ± 2.1 percent). Boars that did not show PGC had a higher proportion of intact acrosomes than those with PGC (86.5 ± 1.2 vs. 80.2 ± 1.1 percent, respectively; P<0.01). Defects in acrosomal integrity were significantly correlated with sperm defects associated to GC (between -0.20 y -0.36; P<0.05). These results suggest that membrane and morphologic defects tend to occur together in the same ejaculate, pointing out the need to build a multifactor seminal quality index that can be used as decision criterion in the swine industry.

Animals , Acrosome , Inclusion Bodies , Swine/genetics , Sperm Capacitation , Semen/chemistry , Veterinary Medicine
Int. braz. j. urol ; 35(3): 299-309, May-June 2009. graf, tab
Article in English | LILACS | ID: lil-523155


INTRODUCTION: Studies that compare the impact of different infectious entities of the male reproductive tract (MRT) on the male accessory gland function are controversial. MATERIAL AND METHODS: Semen analyses of 71 patients with proven infections of the MRT were compared with the results of 40 healthy non-infected volunteers. Patients were divided into 3 groups according to their diagnosis: chronic prostatitis NIH type II (n = 38), chronic epididymitis (n = 12), and chronic urethritis (n = 21). RESULTS: The bacteriological analysis revealed 9 different types of microorganisms, considered to be the etiological agents, isolated in different secretions, including: urine, expressed prostatic secretions, semen and urethral smears: E. Coli (n = 20), Klebsiella (n = 2), Proteus spp. (n = 1), Enterococcus (n = 20), Staphylococcus spp. (n = 1), M. tuberculosis (n = 2), N. gonorrhea (n = 8), Chlamydia tr. (n = 16) and, Ureaplasma urealyticum (n = 1). The infection group had significantly (p < 0.05) lower: semen volume, alpha-glucosidase, fructose, and zinc in seminal plasma and, higher pH than the control group. None of these parameters was sufficiently accurate in the ROC analysis to discriminate between infected and non-infected men. CONCLUSION: Proven bacterial infections of the MRT impact negatively on all the accessory gland function parameters evaluated in semen, suggesting impairment of the secretory capacity of the epididymis, seminal vesicles and prostate. These findings were associated with an infectious related significant increase of semen pH. None of the semen parameters evaluated can be suggested as a diagnostic tool for infection.

Adult , Humans , Male , Middle Aged , Young Adult , Bacterial Infections/complications , Genital Diseases, Male/diagnosis , Genitalia, Male , Semen , Biomarkers/analysis , Case-Control Studies , Chronic Disease , Citric Acid/analysis , Ejaculation/physiology , Epididymitis/diagnosis , Epididymitis/physiopathology , Fructose/analysis , Genital Diseases, Male/microbiology , Genital Diseases, Male/physiopathology , Infertility, Male/microbiology , Prostate/physiopathology , Prostate , Prostatitis/diagnosis , Prostatitis/physiopathology , Semen/chemistry , Semen/microbiology , Seminal Vesicles , Urethritis/diagnosis , Young Adult , alpha-Glucosidases/analysis
Braz. j. vet. res. anim. sci ; 46(1): 54-61, 2009. tab, graf
Article in Portuguese | LILACS | ID: lil-536956


Doze carneiros machos adultos mestiços Santa Inês de mesma idade e porte semelhante foram empregados em um delineamento inteiramente casualizado, por um período experimental de 60 dias. Os animais foram distribuídos para três tratamentos: A. 100% das exigências em proteína degradável no rúmen (controle); B. 100% das exigências em proteína degradável no rúmen + 3% de uréia + enxofre(99% S) e C. 100% das exigências em proteína degradável no rúmen +3% de uréia + enxofre quelatado (21,5% S). Semanalmente foram colhidas amostras de sêmen obtidas com emprego de vagina artificial e de sangue para determinação da concentração de nitrogênio uréico plasmático, assim como realizadas pesagens dos animais e aferições de circunferência escrotal. No sêmen foram analisados: volume e turbilhonamento; vigor, motilidade e concentração espermática; totalde espermatozóides e total de espermatozóides viáveis no ejaculado; integridade de membrana e de acrossoma; morfologia espermática e concentração de nitrogênio uréico no plasma seminal. Os animais suplementados com uréia apresentaram níveis de N-uréico no plasma sanguíneo e seminal significativamente maiores que os encontrados nos do tratamentos controle (p<0,05). Houve diferença significativa entre as fontes de enxofre utilizadas (p<0,05) quanto às características do sêmen estudadas, o tratamento C apresentando valores maiores para turbilhonamento (4,57), motilidade espermática (85,69%), vigor espermático (4,66) e total de espermatozóides por ejaculado (9,02 x109), além de uma porcentagem inferior de defeitos menores (5,37%)quando comparado ao tratamento B

Twelve adult Santa Inês crossbred rams with similar weight and age employed in a randomized design for 60 days period to evaluate three treatments: A. 100% of degradable protein requirement (control); B.100% of degradable protein requirement + 3% urea + inorganic sulphur (99%S) and C. 100% of degradable protein requirement+3% urea + organic sulphur (21,5% S). Every week seminal collections were made through artificial vagina; blood collections were made toanalyze plasma N-ureic levels and measured live weight and scrotal circumference. In semen samples were studied volume, microscopic waves, vigor, motility, concentration, total sperm per ejaculate, total feasible sperm per ejaculate, membrane sperm integrity, acrosomal integrity, percent of abnormal spermatozoa and N-ureic level in seminal plasma. Treatments experimental animals receiving presented blood and seminal plasma N-ureic levels higher than the ones of control treatment (p < 0,05). There was significant difference between organicand inorganic sources of sulphur in the following semen characteristics (p < 0,05): treatment C presented microscopic waves (4,57), motility(85,69%), vigor (4,66) and total sperm per ejaculate (9,02 x 109) higher than treatment B; and the percentage of secondary sperm abnormality (5,37%) was lower than treatment B

Animals , Rumen/physiology , Sheep , Semen/chemistry , Urea/adverse effects
Assiut Medical Journal. 2009; 33 (3): 1-10
in English | IMEMR | ID: emr-135409


Diabetes affects an increasingly large number of young men of reproductive age To determine fertility status and to evaluate semen parameters and sexual dysfunction in men having juvenile onset diabetes comparing them to fertile controls. 73 male patients having juvenile onset diabetes mellitus. The study included clinical evaluation, erectile capacity scoring with IIEF-5 score, urine analysis after masturbation, conventional semen analysis and sperm hypoosmotic swelling test of 73 diabetic men and 33 fertile controls. Comparison between diabetic patients and fertile controls in conventional sperm parameters and hypoosmotic sperm swelling percentage [HOS%]. Additionally, assessment of prevalence of infertility, erectile and ejaculatory dysfunction in diabetic patients. There was a significant decrease in percentage of normal sperm morphology among diabetic patients [41.37 +/- 12. 38] than controls [57.27 +/- 8.11] [P < 0.001]. Percentage of progressive sperm motility was significantly lower in diabetic patients [33.42 +/- 13.38] than controls [54.84 +/- 5.92] [P < 0.001]. There was a significant decrease in sperm HOS% among diabetic patients [62.55 +/- 11.69] in comparison to controls [77.36 +/- 8.23] [P < 0.001]. There was no significant difference in sperm concentration [in million sperm/ml] between diabetic patients [69.75 +/- 62.11] and controls [74.55 +/- 50.78]. Similar results were obtained on comparing between fertile and infertile diabetics. Prevalence of infertility was 40%, erectile dysfunction [ED] was 75%, premature ejaculation was 31% and partial retrograde ejaculation was 5%. Diabetic patients had significantly lower normal sperm morphology, progressive sperm motility and hypoosmotic sperm swelling percentages. They had higher round cell number. They also had increased prevalence of infertility, erectile and ejaculatory dysfunctions

Humans , Male , Semen/chemistry , Sperm Count , Sperm Motility , Erectile Dysfunction , Infertility, Male
Iranian Journal of Veterinary Research. 2008; 63 (2): 41-46
in English | IMEMR | ID: emr-87274


Leptin, known as a potential satiety factor, plays an important role in both metabolism and reproduction. The presence of leptin in human seminal plasma and human spermatozoa has been shown; recently, leptin receptors [Ob-R] have been localized in human spermatozoa, thus suggesting a possible action of this hormone even on these cells. Our aim was to detect leptin receptor mRNA in bull ejaculated spermatozoa by reverse transcriptase-polymerase chain reaction [RT-PCR]. Total RNA was isolated from bull ejaculated spermatozoa and purified by different methods. Our results have revealed that sodium dodecylsulphate [SDS] and SDS/citric acid extraction methods are superior to guanidinium isothiocyanate in terms of yield and reproducibility of RNA recovery. The mRNA for Ob-Rb was detected in all samples examined. We conclude that Ob-R mRNA is present in bull spermatozoa where seminal plasma leptin can exert its effects

Animals , Semen/chemistry , Spermatozoa , Receptors, Leptin/analysis , RNA/isolation & purification , Reverse Transcriptase Polymerase Chain Reaction , RNA, Messenger , Ejaculation
Article in English | IMSEAR | ID: sea-45273


BACKGROUND: Rape is a crime found in Thailand nowadays. The crime is often lacking of eyewitnesses. Therefore, examination for forensic biological evidence becomes quite important, especially investigating sperm and semen in vaginal specimens of the victim. Acid phosphatase test for semen is commonly used in Thailand but is just a presumptive test. Recently, confirmatory kit tests became available in Thailand for detecting the prostate specific antigen (PSA) from semen. This test is simpler and cheaper than ELISA. OBJECTIVE: To compare the rapid one-step immunochromatographic assay with ELISA for the detection of prostate specific antigen in vaginal specimens of raped women. MATERIAL AND METHOD: A diagnostic test was conducted on the vaginal specimens of raped women that were sent to the laboratory of the Pathology Department, Faculty of Medicine, Ramathibodi Hospital, Mahidol University during April-August 2006. One hundred vaginal specimens were examined for prostate specific antigen by rapid one step immunochromatographic assay and compared with ELISA. RESULTS: There were 85% and 83% of sensitivity, 85% and 85% of specificity, 85% and 85% of accuracy, 89% and 89% of positive predictive value, and 79% and 77% of negative predictive value from rapid one-step test kit and ELISA respectively CONCLUSION: The result showed that there was no difference on specificity, accuracy and positive predictive value between the two methods but sensitivity and negative predictive value of rapid one-step test kit was better than ELISA. The research team recommends that rapid one-step test kit for prostate specific antigen should be routine service in vaginal specimens of raped women.

Biological Assay , Enzyme-Linked Immunosorbent Assay/instrumentation , Female , Forensic Pathology , Humans , Male , Predictive Value of Tests , Rape/diagnosis , Semen/chemistry , Thailand , Vagina/chemistry
Indian J Biochem Biophys ; 2007 Dec; 44(6): 437-42
Article in English | IMSEAR | ID: sea-28365


Assisted reproductive technique (ART) has revolutionized the management of severe male factor infertility and in some countries 5% babies are conceived through ART/intra cytoplasmic sperm injection (ICSI). However, the carry-home live birth rate after several ART cycles is low (18-25%) and this is financially, physically and emotionally crippling for the couples. Genetic factors could lead to pre or post-implantation failure and thus explain for low ART success rate. Thus, this study was planned to understand, if infertile men harbour genetic abnormalities which may be iatrogenically transmitted by ART and adversely affect growth potential of embryo. Ninety infertile men underwent semen, cytogenetic, Yq microdeletion and mitochondrial mutation analysis. Of these, 14.4% cases harboured cytogenetic abnormality, and 8.89% Yq microdeletions. A high frequency of mitochondrial mutations was found in 23 men with asthenospermia. It is important to understand that through ART genetic abnormalities are transmitted to offspring, resulting in impaired growth and development potential of embryo and poor take-home live birth rate. Thus, genetic analysis is strongly recommend in all men with idiopathic infertility who opt for ART to counsel couples and provide them with most adapted therapeutics.

Adult , Cell Nucleus/genetics , Chromosome Deletion , Chromosomes, Human, Y/genetics , Gene Amplification , Genome , Humans , Infertility, Male/diagnosis , Male , Mitochondria/genetics , Mutation , Reproductive Techniques, Assisted , Semen/chemistry , Sperm Injections, Intracytoplasmic
Article in English | IMSEAR | ID: sea-43014


OBJECTIVE: The present study was designed to determine the effect of the freeze-thawing procedure, computer controlled rate freezing and duration for six months, on human sperm chromatin (assessed by acridine orange test), vitality, motility, and morphology. DESIGN: Experimental study MATERIAL AND METHOD: Twenty semen samples were obtained from patients attending the infertility unit. The semen analysis was measured according to WHO criteria. Sperm morphology was evaluated by strict Kruger criteria and sperm chromatin were detected by acridine orange test. After semen analysis, each sample was mixed with cryoprotectant and divided into straw. The straw was frozen with computer controlled rate freezing method After 6 months of cryostorage, semen samples were thawed and then the semen was analyed, and sperm chromatin and morphology were determined. RESULTS: After six months of cryostorage, the mean percentage of normal sperm chromatin decreased significantly (87.3 +/- 9.0 vs. 51.9 +/- 27.4, p < 0.001). Vitality, motility, and normal morphology of sperm decreased significantly (78.7 +/- 1.9 vs. 32.8 + 10.8, 52.6 + 1.9 vs. 24.1 +/- 10.9 and 21.4 +/- 4.3 vs. 18.0 +/- 4.4 respectively, p < 0.001). CONCLUSIONS: The computer controlled rate freezing of sperm for six months and thawing process significantly decreased normal sperm chromatin, vitality, motility, and normal morphology.

Chromatin , Cryopreservation , Cryoprotective Agents , DNA Fragmentation , Humans , Male , Pilot Projects , Semen/chemistry , Semen Preservation , Sperm Motility , Spermatozoa , Tissue Preservation