Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 164
Filter
1.
Neuroscience Bulletin ; (6): 1289-1302, 2021.
Article in English | WPRIM | ID: wpr-922623

ABSTRACT

Growth differentiation factor 15 (GDF-15) is a member of the transforming growth factor-β superfamily. It is widely distributed in the central and peripheral nervous systems. Whether and how GDF-15 modulates nociceptive signaling remains unclear. Behaviorally, we found that peripheral GDF-15 significantly elevated nociceptive response thresholds to mechanical and thermal stimuli in naïve and arthritic rats. Electrophysiologically, we demonstrated that GDF-15 decreased the excitability of small-diameter dorsal root ganglia (DRG) neurons. Furthermore, GDF-15 concentration-dependently suppressed tetrodotoxin-resistant sodium channel Nav1.8 currents, and shifted the steady-state inactivation curves of Nav1.8 in a hyperpolarizing direction. GDF-15 also reduced window currents and slowed down the recovery rate of Nav1.8 channels, suggesting that GDF-15 accelerated inactivation and slowed recovery of the channel. Immunohistochemistry results showed that activin receptor-like kinase-2 (ALK2) was widely expressed in DRG medium- and small-diameter neurons, and some of them were Nav1.8-positive. Blockade of ALK2 prevented the GDF-15-induced inhibition of Nav1.8 currents and nociceptive behaviors. Inhibition of PKA and ERK, but not PKC, blocked the inhibitory effect of GDF-15 on Nav1.8 currents. These results suggest a functional link between GDF-15 and Nav1.8 in DRG neurons via ALK2 receptors and PKA associated with MEK/ERK, which mediate the peripheral analgesia of GDF-15.


Subject(s)
Analgesia , Animals , Ganglia, Spinal , Growth Differentiation Factor 15 , Rats , Sensory Receptor Cells , Sodium Channels , Tetrodotoxin/pharmacology
2.
Arq. bras. neurocir ; 37(4): 317-325, 15/12/2018.
Article in English | LILACS | ID: biblio-1362634

ABSTRACT

Background and Objective Various irradiances have been reported to be beneficial for the treatment of neuropathic pain with near infrared light. However, the mechanistic basis for the beneficial outcomes may vary based on the level of irradiance or fluence rate used. Using in vivo and in vitro experimentalmodels, this study determined the mechanistic basis of photobiomodulation therapy (PBMT) for the treatment of neuropathic pain using a high irradiance. Study Design/Materials and Methods ln vitro experiments: Cultured, rat DRG were randomly assigned to control or laser treatment (L T) groups with different irradiation times (2, 5, 30, 60 or 120s). The laser parameters were: output power » 960 mW, irradiance » 300mW/cm2, 808 nm wavelength and spot size » 3cm diameter/ area » 7.07cm2, with different fluences according to irradiation times. Mitochondrial metabolic activity was measured with the MTS assay. The DRG neurons were immunostained using a primary antibody to ß-Tubulin III. ln vivo experiments: spared nerve injury surgery (SNI), an animal model of persistent peripheral neuropathic pain, was used. The injured rats were randomly divided into three groups (n » 5). 1) Control: SNI without LT, 2) Short term: SNI with LT on day 7 and euthanized on day 7, 3) Long term: SNI with LT on day 7 and euthanized on day 22. An 808 nm wavelength laser was used for all treatment groups. Treatment was performed once on Day 7 post-surgery. The transcutaneous treatment parameters were: output power: 10 W, fluence rate: 270 mW/cm2, treatment time: 120s. The laser probe was moved along the course of the sciatic/sural nerve during the treatment. Within 1 hour of irradiation, behavior tests were performed to assess its immediate effect on sensory allodynia and hyperalgesia caused by SNI. Results ln vitro experiments: Mitochondrial metabolism was significantly lower compared with controls for all LT groups. Varicosities and undulations formed in neurites of DRG neurons with a cell body diameter 30µm or less. ln neurites of DRG neurons with a cell body diameter of greater than 30µm, varicosities formed only in the 120s group. ln vivo experiments: For heat hyperalgesia, there was a statistically significant reduction in sensitivity to the heat stimulus compared with the measurements done on day 7 prior to LT. A decrease in the sensitivity to the heat stimulus was found in the LT groups compared with the control group on day 15 and 21. For cold allodynia and mechanical hyperalgesia, a significant decrease in sensitivity to cold and pin prick was found within 1 hour after L T. Sensitivity to these stimuli returned to the control levels after 5 days post-L T. No significant difference was found in mechanical allodynia between control and L T groups for all time points examined. Conclusion These in vitro and in vivo studies indicate that treatment with an irradiance/fluence rate at 270 m W/cm2 or higher at the level of the nerve can rapidly block pain transmission. A combination therapy is proposed to treat neuropathic pain with initial high irradiance/fluence rates for fast pain relief, followed by low irradiance/ fluence rates for prolonged pain relief by altering chronic inflammation.


Subject(s)
Animals , Rats , Sensory Receptor Cells/metabolism , Low-Level Light Therapy/statistics & numerical data , Ganglia, Spinal , Hyperalgesia/therapy , Neuralgia/therapy , In Vitro Techniques/methods , Immunohistochemistry/methods , Analysis of Variance , Nerve Regeneration
3.
Salud pública Méx ; 60(4): 472-478, Jul.-Aug. 2018. graf
Article in Spanish | LILACS | ID: biblio-979161

ABSTRACT

Resumen Objetivo Describir el papel de la percepción del gusto como factor de riesgo para el desarrollo de obesidad en niños. Material y métodos Se realizó una búsqueda inicial de artículos científicos publicados en PubMed entre el 1 de enero de 2011 y el 20 de marzo de 2016 para el tema sobrepeso y obesidad en niños de entre 0 y 12 años. Los algoritmos utilizados fueron (Obesity OR Overweight) AND Taste perception, Satiation, Satiety response, Appetite, Appetite regulation, Habituation, Taste receptors [MeSH] y PROP phenotype. En búsquedas subsecuentes se incluyeron artículos previos y posteriores a la fecha de la búsqueda general (hasta mayo 2018). Resultados Las preferencias por los sabores inician desde la gestación, por lo que los niños que son expuestos a sabores dulces en etapas tempranas de la infancia aumentan su riesgo de habituación a éstos. Asimismo, las experiencias hedónicas dadas por la ingestión de alimentos y bebidas dulces refuerzan el consumo de estos alimentos, lo que propicia la selección de productos o bebidas de sabor dulce en etapas posteriores. Estas preferencias se han asociado con el desarrollo de obesidad en los niños. Las variantes genéticas relacionadas con la percepción del gusto también pueden contribuir a la selección de cierto tipo de alimentos. Sin embargo, su relación con una mayor ingestión de energía, así como con un mayor peso corporal, ha sido poco explorada y ha mostrado resultados inconsistentes. Conclusiones Se requiere más evidencia para entender las interacciones ambientales y genéticas de la percepción del gusto, a fin de considerarlo un factor más en las intervenciones de política pública.


Abstract Objective To describe the role of taste perception in the development of sweet taste habituation as well as its relationship to the development of obesity in children. Materials and methods An initial search of scientific articles published in PubMed between January 1st, 2011 and March 20th, 2016 was performed in children between 0 and 12 years old. The algorithms used were (Obesity OR Overweight) AND (Taste perception, Satiation, Satiety response, Appetite, Appetite regulation Habituation, Taste receptors [MeSH]) and PROP phenotype. Subsequent searches included papers published before and after date of initial search (until May 2018). Results Flavor preferences start as early as taste system development during pregnancy. Therefore, children who are exposed to sweet flavors in early childhood, increase their risk of habituation to them. Likewise, the hedonic experiences given by the ingestion of sweet foods and beverages, reinforce the consumption of these foods, perpetuating their selection in later stages. Preference for sweet taste has been associated with the development of obesity in children. Functional genetic variants related to taste perception can also contribute to the selection of certain types of foods and there is enough evidence that supports this idea. However, its contribution to a higher energy intake as well as a higher body weight has been poorly explored with inconsistent results. Conclusions More evidence is required to understand the environmental and genetic interactions of taste perception, so in turn, it can be consider as a key factor for preventing child obesity.


Subject(s)
Humans , Male , Pregnancy , Infant, Newborn , Infant , Child, Preschool , Child , Taste Perception , Pediatric Obesity/epidemiology , Food Preferences , Prenatal Exposure Delayed Effects , Sensory Receptor Cells/physiology , Satiety Response , Energy Intake , Risk Factors , Feeding Behavior , Pediatric Obesity/etiology , Pediatric Obesity/psychology , Habituation, Psychophysiologic
4.
Prensa méd. argent ; 104(1): 3-18, 20180000. fig
Article in Spanish | LILACS, BINACIS | ID: biblio-1370807

ABSTRACT

El sistema nervioso autónomo (SNA) es un componente fundamental del sistema nervioso cuya función es mantener la homeostasis y reaccionar de forma adaptativa a los cambios en el medio externo e interno. Participa en la regulación de la respiración, la circulación, la digestión, el metabolismo y el medio interno, la secreción exocrina y endocrina, las respuestas inmunes, la temperatura corporal y la reproducción. En este trabajo se analizará cómo la organización del SNA se construye en 4 niveles jerárquicos, a partir de un periodo de diferenciación crítico neonatal en el cual el medio ambiente y el vínculo afectivo con la madre juega un rol predominante. A continuación, se discutirá cómo la función del SNA cambia en las tres configuraciones corporales (vigilia, sueño de ondas lentas, sueño de movimientos oculares rápidos, REM) que se suceden durante un ciclo de 24 horas. Por último, se discutirá la aplicación de estos conceptos en la Unidad Neurofisiología del Curso de Fisiología para alumnos de 2º año de la Facultad de Ciencias Médicas, UCA, enfatizando los aspectos instrumentales destinados a aumentar la participación de los alumnos en el proceso de enseñanza


The autonomic nervous system (ANS) is a fundamental component of the nervous system whose function is to maintain homeostasis and react adaptively to changes in the external and internal environment. It participates in the regulation of respiration, circulation, digestion, metabolism and internal environment, exocrine and endocrine secretion, immune response, body temperature and reproduction. In this review article I will analyze how the organization of the ANS is built on 4 hierarchical levels, starting from a period of critical neonatal differentiation in which the environment and the affective bond with the mother plays a predominant role. Next, I will discuss how the ANS function changes in the three body configurations (wakefulness, slow wave sleep, fast eye movement, REM) that occur during a 24 hour cycle. Finally, the application of these concepts to teaching Neurophysiology at the Physiology Course for 2nd year medical students of the Faculty of Medical Sciences, Pontificia Universidad Católica Argentina, emphasizing the instrumental aspects intended to increase the participation of students in the teaching process is discussed.


Subject(s)
Humans , Sensory Receptor Cells/physiology , Autonomic Nervous System/physiology , Models, Biopsychosocial , Limbic System/physiology
5.
Neuroscience Bulletin ; (6): 156-164, 2018.
Article in English | WPRIM | ID: wpr-777082

ABSTRACT

Peripheral itch stimuli are transmitted by sensory neurons to the spinal cord dorsal horn, which then transmits the information to the brain. The molecular and cellular mechanisms within the dorsal horn for itch transmission have only been investigated and identified during the past ten years. This review covers the progress that has been made in identifying the peptide families in sensory neurons and the receptor families in dorsal horn neurons as putative itch transmitters, with a focus on gastrin-releasing peptide (GRP)-GRP receptor signaling. Also discussed are the signaling mechanisms, including opioids, by which various types of itch are transmitted and modulated, as well as the many conflicting results arising from recent studies.


Subject(s)
Action Potentials , Analgesics, Opioid , Pharmacology , Animals , Humans , Pruritus , Metabolism , Pathology , Sensory Receptor Cells , Metabolism , Spinal Cord , Pathology , Synaptic Transmission , Physiology
6.
Neuroscience Bulletin ; (6): 178-185, 2018.
Article in English | WPRIM | ID: wpr-777081

ABSTRACT

Chronic pain and itch are a pathological operation of the somatosensory system at the levels of primary sensory neurons, spinal cord and brain. Pain and itch are clearly distinct sensations, and recent studies have revealed the separate neuronal pathways that are involved in each sensation. However, the mechanisms by which these sensations turn into a pathological chronic state are poorly understood. A proposed mechanism underlying chronic pain and itch involves abnormal excitability in dorsal horn neurons in the spinal cord. Furthermore, an increasing body of evidence from models of chronic pain and itch has indicated that synaptic hyperexcitability in the spinal dorsal horn might not be a consequence simply of changes in neurons, but rather of multiple alterations in glial cells. Thus, understanding the key roles of glial cells may provide us with exciting insights into the mechanisms of chronicity of pain and itch, and lead to new targets for treating chronic pain and itch.


Subject(s)
Animals , Chronic Pain , Pathology , Humans , Neuralgia , Metabolism , Pruritus , Pathology , Sensory Receptor Cells , Physiology , Spinal Cord , Pathology
7.
Neuroscience Bulletin ; (6): 200-207, 2018.
Article in English | WPRIM | ID: wpr-777071

ABSTRACT

Different physical and chemical stimuli are detected by the peripheral sensory receptors of dorsal root ganglion (DRG) neurons, and the generated inputs are transmitted via afferent fibers into the central nervous system. The gene expression profiles of DRG neurons contribute to the generation, transmission, and regulation of various somatosensory signals. Recently, the single-cell transcriptomes, cell types, and functional annotations of somatosensory neurons have been studied. In this review, we introduce our classification of DRG neurons based on single-cell RNA-sequencing and functional analyses, and discuss the technical approaches. Moreover, studies on the molecular and cellular mechanisms underlying somatic sensations are discussed.


Subject(s)
Animals , Ganglia, Spinal , Cell Biology , Gene Regulatory Networks , Humans , Pain , Genetics , Metabolism , Pathology , Sensory Receptor Cells , Metabolism , Sequence Analysis, RNA , Transcriptome
8.
Neuroscience Bulletin ; (6): 22-41, 2018.
Article in English | WPRIM | ID: wpr-777048

ABSTRACT

The voltage-gated Na channel subtype Nav1.7 is important for pain and itch in rodents and humans. We previously showed that a Nav1.7-targeting monoclonal antibody (SVmab) reduces Na currents and pain and itch responses in mice. Here, we investigated whether recombinant SVmab (rSVmab) binds to and blocks Nav1.7 similar to SVmab. ELISA tests revealed that SVmab was capable of binding to Nav1.7-expressing HEK293 cells, mouse DRG neurons, human nerve tissue, and the voltage-sensor domain II of Nav1.7. In contrast, rSVmab showed no or weak binding to Nav1.7 in these tests. Patch-clamp recordings showed that SVmab, but not rSVmab, markedly inhibited Na currents in Nav1.7-expressing HEK293 cells. Notably, electrical field stimulation increased the blocking activity of SVmab and rSVmab in Nav1.7-expressing HEK293 cells. SVmab was more effective than rSVmab in inhibiting paclitaxel-induced mechanical allodynia. SVmab also bound to human DRG neurons and inhibited their Na currents. Finally, potential reasons for the differential efficacy of SVmab and rSVmab and future directions are discussed.


Subject(s)
Animals , Antibodies, Monoclonal , Therapeutic Uses , Biotin , Metabolism , Cells, Cultured , Disease Models, Animal , Female , Ganglia, Spinal , Cell Biology , HEK293 Cells , Humans , Hybridomas , Chemistry , Hyperalgesia , Drug Therapy , Male , Mice , Mice, Inbred C57BL , Metabolism , Chemistry , Allergy and Immunology , Metabolism , Neuralgia , Drug Therapy , Metabolism , Protein Binding , Recombinant Proteins , Therapeutic Uses , Sensory Receptor Cells , Physiology
9.
Article in English | WPRIM | ID: wpr-718135

ABSTRACT

Chronic cough is common in the community and causes significant morbidity. Several factors may underlie this problem, but comorbid conditions located at sensory nerve endings that regulate the cough reflex, including rhinitis, rhinosinusitis, asthma, eosinophilic bronchitis, and gastroesophageal reflux disease, are considered important. However, chronic cough is frequently non-specific and accompanied by not easily identifiable causes during the initial evaluation. Therefore, there are unmet needs for developing empirical treatment and practical diagnostic approaches that can be applied in primary clinics. Meanwhile, in referral clinics, a considerable proportion of adult patients with chronic cough are unexplained or refractory to conventional treatment. The present clinical practice guidelines aim to address major clinical questions regarding empirical treatment, practical diagnostic tools for non-specific chronic cough, and available therapeutic options for chronic wet cough in children and unexplained chronic cough in adults in Korea.


Subject(s)
Adult , Asthma , Bronchitis , Child , Cough , Eosinophils , Evidence-Based Medicine , Gastroesophageal Reflux , Humans , Korea , Referral and Consultation , Reflex , Rhinitis , Sensory Receptor Cells
10.
Protein & Cell ; (12): 644-661, 2017.
Article in English | WPRIM | ID: wpr-756997

ABSTRACT

Cutaneous neurogenic inflammation (CNI) is inflammation that is induced (or enhanced) in the skin by the release of neuropeptides from sensory nerve endings. Clinical manifestations are mainly sensory and vascular disorders such as pruritus and erythema. Transient receptor potential vanilloid 1 and ankyrin 1 (TRPV1 and TRPA1, respectively) are non-selective cation channels known to specifically participate in pain and CNI. Both TRPV1 and TRPA1 are co-expressed in a large subset of sensory nerves, where they integrate numerous noxious stimuli. It is now clear that the expression of both channels also extends far beyond the sensory nerves in the skin, occuring also in keratinocytes, mast cells, dendritic cells, and endothelial cells. In these non-neuronal cells, TRPV1 and TRPA1 also act as nociceptive sensors and potentiate the inflammatory process. This review discusses the role of TRPV1 and TRPA1 in the modulation of inflammatory genes that leads to or maintains CNI in sensory neurons and non-neuronal skin cells. In addition, this review provides a summary of current research on the intracellular sensitization pathways of both TRP channels by other endogenous inflammatory mediators that promote the self-maintenance of CNI.


Subject(s)
Animals , Chronic Disease , Dendritic Cells , Metabolism , Pathology , Dermatitis , Metabolism , Pathology , Gene Expression Regulation , Humans , Inflammation , Metabolism , Pathology , Keratinocytes , Metabolism , Pathology , Mast Cells , Metabolism , Pathology , Sensory Receptor Cells , Metabolism , Pathology , TRPA1 Cation Channel , TRPV Cation Channels
11.
Article in English | WPRIM | ID: wpr-728585

ABSTRACT

Remote ischemic preconditioning (RIPC) is an intrinsic phenomenon whereby 3~4 consecutive ischemia-reperfusion cycles to a remote tissue (noncardiac) increases the tolerance of the myocardium to sustained ischemiareperfusion induced injury. Remote ischemic preconditioning induces the local release of chemical mediators which activate the sensory nerve endings to convey signals to the brain. The latter consequently stimulates the efferent nerve endings innervating the myocardium to induce cardioprotection. Indeed, RIPC-induced cardioprotective effects are reliant on the presence of intact neuronal pathways, which has been confirmed using nerve resection of nerves including femoral nerve, vagus nerve, and sciatic nerve. The involvement of neurogenic signaling has been further substantiated using various pharmacological modulators including hexamethonium and trimetaphan. The present review focuses on the potential involvement of neurogenic pathways in mediating remote ischemic preconditioning-induced cardioprotection.


Subject(s)
Brain , Femoral Nerve , Hexamethonium , Ischemic Preconditioning , Myocardium , Negotiating , Nerve Endings , Neurons , Sciatic Nerve , Sensory Receptor Cells , Trimethaphan , Vagus Nerve
12.
Article in English | WPRIM | ID: wpr-162044

ABSTRACT

BACKGROUND/AIMS: Patients with long-standing diabetes often demonstrate intestinal dysfunction and abdominal pain. However, the pathophysiology of abdominal pain in diabetic patients remains elusive. The purpose of study was to determine roles of voltage-gated sodium channels in dorsal root ganglion (DRG) in colonic hypersensitivity of rats with diabetes. METHODS: Diabetic models were induced by a single intraperitoneal injection of streptozotocin (STZ; 65 mg/kg) in adult female rats, while the control rats received citrate buffer only. Behavioral responses to colorectal distention were used to determine colonic sensitivity in rats. Colon projection DRG neurons labeled with DiI were acutely dissociated for measuring excitability and sodium channel currents by whole-cell patch clamp recordings. Western blot analysis was employed to measure the expression of NaV1.7 and NaV1.8 of colon DRGs. RESULTS: STZ injection produced a significantly lower distention threshold than control rats in responding to colorectal distention. STZ injection also depolarized the resting membrane potentials, hyperpolarized action potential threshold, decreased rheobase and increased frequency of action potentials evoked by 2 and 3 times rheobase and ramp current stimulation. Furthermore, STZ injection enhanced neuronal sodium current densities of DRG neurons innervating the colon. STZ injection also led to a significant upregulation of NaV1.7 and NaV1.8 expression in colon DRGs compared with age and sex-matched control rats. CONCLUSIONS: Our results suggest that enhanced neuronal excitability following STZ injection, which may be mediated by upregulation of NaV1.7 and NaV1.8 expression in DRGs, may play an important role in colonic hypersensitivity in rats with diabetes.


Subject(s)
Abdominal Pain , Action Potentials , Adult , Animals , Architectural Accessibility , Blotting, Western , Citric Acid , Colon , Diagnosis-Related Groups , Female , Ganglia, Spinal , Humans , Hypersensitivity , Injections, Intraperitoneal , Membrane Potentials , Neurons , Rats , Sensory Receptor Cells , Sodium , Sodium Channels , Streptozocin , Up-Regulation , Voltage-Gated Sodium Channels
14.
Immune Network ; : 233-241, 2016.
Article in English | WPRIM | ID: wpr-97831

ABSTRACT

DCs, like the sensory neurons, express vanilloid receptor 1 (VR1). Here we demonstrate that the VR1 agonists, capsaicin (CP) and resiniferatoxin (RTX), enhance antiviral CTL responses by increasing MHC class I-restricted viral antigen presentation in dendritic cells (DCs). Bone marrow-derived DCs (BM-DCs) were infected with a recombinant vaccinia virus (VV) expressing OVA (VV-OVA), and then treated with CP or RTX. Both CP and RTX increased MHC class I-restricted presentation of virus-encoded endogenous OVA in BM-DCs. Oral administration of CP or RTX significantly increased MHC class I-restricted OVA presentation by splenic and lymph node DCs in VV-OVA-infected mice, as assessed by directly measuring OVA peptide SIINFEKL-Kb complexes on the cell surface and by performing functional assays using OVA-specific CD8 T cells. Accordingly, oral administration of CP or RTX elicited potent OVA-specific CTL activity in VV-OVA-infected mice. The results from this study demonstrate that VR1 agonists enhance anti-viral CTL responses, as well as a neuro-immune connection in anti-viral immune responses.


Subject(s)
Administration, Oral , Animals , Antigen Presentation , Capsaicin , Dendritic Cells , Lymph Nodes , Mice , Ovum , Sensory Receptor Cells , T-Lymphocytes , Vaccinia virus
15.
Arq. ciênc. vet. zool. UNIPAR ; 19(1): 41-45, jan.-mar. 2016. tab
Article in Portuguese | LILACS, VETINDEX | ID: biblio-833787

ABSTRACT

O processo de deterioração do pescado é facilitado por suas características intrínsecas, como a proximidade da neutralidade que se encontra o pH; ação das enzimas autolíticas; elevada atividade de água e presença de nutrientes; além da elevada quantidade de gorduras insaturadas que auxiliam agilizando o processo de formação do ranço. Por isso, o emprego dos métodos de conservação deve ser realizado o mais prontamente possível, retardando a instalação da deterioração e mantendo o produto fresco por um período maior. Entre as tecnologias disponíveis para a conservação estão as radiações ionizantes. A irradiação de alimentos já é utilizada em vários países, sendo eficaz na extensão da validade comercial e até mesmo na melhora de alguns atributos sensoriais. Objetivou-se no presente trabalho contribuir para a avaliação da interferência da irradiação por feixe de elétrons nas características sensoriais de filés de corvina (M. furnieri) refrigerados, desembarcados no município de Niterói - RJ, Brasil. As amostras foram divididas em três grupos: controle; irradiado a 0,7 kGy; e irradiado a 1,0 kGy, os quais foram comparados sensorialmente entre si através de testes triangulares realizados com 30 provadores cada. Foram avaliados aroma, sabor, odor, textura e "outros". Os peixes inteiros foram adquiridos no cais de Itaipú, filetados no mercado, embalados a vácuo e mantidos à ±4°C. Foi encontrada diferença estatisticamente significativa (p<0,05) entre as amostras, quando confrontado o grupo irradiado a 1,0 kGy com os outros dois grupos. Concluiu-se portanto, que a dose de 1,0 kGy alterou as características sensoriais dos filés de corvina irradiados.(AU)


The process of spoilage of fish is facilitated by its intrinsic features, such as the proximity of neutrality of its pH; action of autolytic enzymes; high presence of water and nutrients; as well as a high amount of unsaturated fats that helps accelerate the process of rancidity. Therefore, preservation methods should be used as promptly as possible, delaying the installation of deterioration and keeping the product fresh for a longer period. Ionizing radiation is one of the technologies available for conservation. Food irradiation is already used in several countries, being effective for the improvement of some sensory attributes and even to extent the commercial validity. The objective of the present work is to contribute to the evaluation of the interference of electron beam irradiation on the sensory characteristics of chilled croaker (M. furnieri) fillets, unloaded in Niterói - RJ, Brazil. The samples were divided into three groups: control; irradiated at 0.7 KGy; and irradiated at 1.0 kGy, which were compared with each other through sensory tests with 30 triangular panelists each. They evaluated aroma, flavor, odor, texture and "other". Whole fish were purchased at Itaipú pier, were fillet in the market, vacuum packed and kept at ± 4 °C. Statistically significant differences were found (p<0,05) between samples when comparing the 1.0-kGy irradiated group with the other groups. It is therefore concluded that a 1.0-kGy dose alters the sensory characteristics of irradiated croaker fillets.(AU)


El proceso de deterioro de peces se facilita por sus características intrínsecas, tales como la proximidad de la neutralidad que se encuentra el PH; acción de las enzimas autolíticas; alta actividad de agua y presencia de nutrientes; además de la alta cantidad de grasas insaturadas que ayudan a acelerar el proceso de formación de rancidez. El uso de métodos de conservación debe realizarse lo más temprano posible, retardando la instalación de deterioro y manteniendo el producto fresco durante un largo espacio de tiempo. Entre las tecnologías disponibles para la conservación están las radiaciones ionizantes. Esta tecnología ya se utiliza en varios países y es muy eficaz en la ampliación de la validad comercial e incluso la mejora de algunos atributos sensoriales. El objetivo del presente trabajo ha sido evaluar el impacto de la irradiación por rayos de electrones en las características sensoriales de filetes de corvina (M. furnieri) refrigerados, desembarcados en el municipio de Niterói - RJ, Brasil. Las muestras se dividieron en tres grupos: control; irradiados a 0.7 kGy; e irradiados a 1,0 kGy, los cuales fueron comparados sensorialmente entre sí a través de pruebas triangulares realizadas con 30 panelistas. También, se evaluaron el aroma, sabor, olor, textura y "otros". Los pescados enteros fueron comprados en el muelle de Itaipú, fileteados en el mercado, envasados al vacío y mantenidos a ± 4°C. Se encontraron diferencias estadísticamente significativas (p<0,05) entre las muestras, cuando se compara el grupo irradiado a 1,0 kGy con los demás. Se concluye que una dosis de 1,0 kGy altera las características sensoriales de filetes de corvina irradiados.(AU)


Subject(s)
Animals , Fishes/anatomy & histology , Fishes/classification , Radiation , Sensory Receptor Cells/classification
16.
Article in English | WPRIM | ID: wpr-728434

ABSTRACT

TWIK-related K+ channel-2 (TREK-2) and TWIK-related spinal cord K+ (TRESK) channel are members of two-pore domain K+ channel family. They are well expressed and help to set the resting membrane potential in sensory neurons. Modulation of TREK-2 and TRESK channels are involved in the pathogenesis of pain, and specifi c activators of TREK-2 and TRESK may be benefi cial for the treatment of pain symptoms. However, the effect of commonly used analgesics on TREK-2 and TRESK channels are not known. Here, we investigated the effect of analgesics on TREK-2 and TRESK channels. The effects of analgesics were examined in HEK cells transfected with TREK-2 or TRESK. Amitriptyline, citalopram, escitalopram, and fluoxetine significantly inhibited TREK-2 and TRESK currents in HEK cells (p<0.05, n=10). Acetaminophen, ibuprofen, nabumetone, and bupropion inhibited TRESK, but had no effect on TREK-2. These results show that all analgesics tested in this study inhibit TRESK activity. Further study is needed to identify the mechanisms by which the analgesics modulate TREK-2 and TRESK differently.


Subject(s)
Acetaminophen , Amitriptyline , Analgesics , Antidepressive Agents , Bupropion , Citalopram , Fluoxetine , Humans , Ibuprofen , Membrane Potentials , Potassium Channels, Tandem Pore Domain , Sensory Receptor Cells , Spinal Cord
17.
Article in English | WPRIM | ID: wpr-38875

ABSTRACT

Herpes zoster most commonly occurs in elderly patients, and usually affects sensory neurons. Therefore, its characteristic symptoms are segmental pain, itching, and sensory changes in the affected areas. A 71-yr-old woman experienced painful herpetic rash on the right cervical 2-4 dermatomes for 16 days. Two days after the onset of the rash, she was diagnosed with herpes zoster, and prescribed 250 mg famciclovir three times a day for 7 days, pregabalin 150 mg twice a day, and tramadol 150 mg once a day for 14 days, by a dermatologist. Despite medication, her pain was rated at an intensity of 6/10 on the numeric rating scale. In addition, she complained of severe itching sensation on the affected dermatomes. Superficial cervical plexus block (SCPB) was performed at the right C4 level with 15 ml 0.5% lidocaine plus triamcinolone 30 mg. Five days after the procedure, pain and itching completely disappeared. SCPB may be an effective option for the treatment of acute pain and itching arising from herpes zoster, and for the prevention of postherpetic neuralgia.


Subject(s)
Acute Pain , Aged , Cervical Plexus Block , Cervical Plexus , Exanthema , Female , Herpes Zoster , Humans , Lidocaine , Neuralgia, Postherpetic , Pregabalin , Pruritus , Sensation , Sensory Receptor Cells , Tramadol , Triamcinolone
18.
Article in English | WPRIM | ID: wpr-34093

ABSTRACT

OBJECTIVES: In mammals, cochlear hair cell loss is irreversible and may result in a permanent sensorineural hearing loss. Secondary to this hair cell loss, a progressive loss of spiral ganglion neurons (SGNs) is presented. In this study, we have investigated the effects of neural-induced human mesenchymal stem cells (NI-hMSCs) from human bone marrow on sensory neuronal regeneration from neomycin treated deafened guinea pig cochleae. METHODS: HMSCs were isolated from the bone marrow which was obtained from the mastoid process during mastoidectomy for ear surgery. Following neural induction with basic fibroblast growth factor and forskolin, we studied the several neural marker and performed electrophysiological analysis. NI-hMSCs were transplanted into the neomycin treated deafened guinea pig cochlea. Engraftment of NI-hMSCs was evaluated immunohistologically at 8 weeks after transplantation. RESULTS: Following neural differentiation, hMSCs expressed high levels of neural markers, ionic channel markers, which are important in neural function, and tetrodotoxin-sensitive voltage-dependent sodium currents. After transplantation into the scala tympani of damaged cochlea, NI-hMSCs-injected animals exhibited a significant increase in the number of SGNs compared to Hanks balanced salt solution-injected animals. Transplanted NI-hMSCs were found within the perilymphatic space, the organ of Corti, along the cochlear nerve fibers, and in the spiral ganglion. Furthermore, the grafted NI-hMSCs migrated into the spiral ganglion where they expressed the neuron-specific marker, NeuN. CONCLUSION: The results show the potential of NI-hMSCs to give rise to replace the lost cochlear cells in hearing loss mammals.


Subject(s)
Animals , Bone Marrow , Cell Differentiation , Cochlea , Cochlear Nerve , Colforsin , Ear , Fibroblast Growth Factor 2 , Guinea Pigs , Hair , Hearing Loss , Hearing Loss, Sensorineural , Humans , Ion Channels , Mammals , Mastoid , Mesenchymal Stem Cells , Neomycin , Neurons , Organ of Corti , Regeneration , Scala Tympani , Sensory Receptor Cells , Sodium , Spiral Ganglion , Transplantation , Transplants
19.
Belo Horizonte; s.n; 2015. 163 p. tab, ilus.
Thesis in Portuguese | LILACS, BDENF | ID: biblio-831455

ABSTRACT

Pacientes com câncer estão sujeitos a diversos efeitos tóxicos decorrentes do tratamento quimioterápico, incluindo a toxicidade neuro sensorial. A percepção sensorial tátil perturbada (PSTP) é considerada uma resposta do paciente ao tratamento antineoplásico o que torna este fenômeno relevante para a praticado enfermeiro. Apesar do impacto que esta questão traz às atividades de vida diária e qualidade de vida do paciente oncológico, a sua representatividade na NANDA-I ainda é inexistente, dificultando a assistência de enfermagem para esta população. Trata-se de estudo realizado em duas etapas: revisão integrativa da literatura e coorte não concorrente para atender o objetivo de validar o diagnóstico de enfermagem PSTP em pacientes adultos oncológicos sob quimioterapia. Dos artigos analisados 46 foram selecionados, a maioria(34,8%) estudos de coorte (Nível de Evidência III), realizados nos EUA (32,6%)e publicados na língua inglesa (98%). Os fatores relacionados (uso de quimioterapia neurotóxica, acúmulo de dose ao longo do tratamento) e as características definidoras (formigamento e dormência, especialmente em extremidades como mãos e pés) mais relevantes foram identificados e compuseram o instrumento utilizado na coorte. Foram incluídos no estudo 127pacientes entre junho de 2006 até dezembro de 2013, que revelou incidência global de PSTP de 57%. O modelo de predição para o desenvolvimento da PSTP foi obtido pela regressão de Cox encontrando-se ao final as variáveis que se mostraram significativas como alcoolismo, presença de metástases,radioterapia prévia, uso de anti-helmínticos, quimioterapia paliativa e desconforto nos membros inferiores. Os resultados revelam que a PSTP é um achado comum em pacientes oncológicos durante a quimioterapia antineoplásica.


Cancer patients may develop many chemotherapy toxic effects, including neurosensory toxicity. The disturbed tactile perception (PSTP) is considered apatient's response to anticancer treatment making this phenomenon relevant for the nursing practice. Despite the influence of this problem in patient's daily activities and quality of life, their representativeness in NANDA-I is still lacking,hampering for nursing care in this population. This is an integrative literature review studyand non-concurrent cohort that aimed validate the diagnosis of nursing diagnoses PSTP in adult cancer patients undergoing chemotherapy. Articles were analyzed and 46 were selected, most (34.8%) cohort studies (LoEIII), conducted in the US (32.6%) and published in English (98%). The most relevant related factors (use of neurotoxic chemotherapy, dose accumulation during treatment) and defining characteristics (tingling and numbness, especially on extremities like hands and feet) were identified and composed the cohort instrument. A total of 127 patients were included in the study from June2006 until December 2013, which revealed 57% overall incidence of PSTP. The prediction model of PSTP development was obtained by Cox regression, andthe significant variables identified were alcoholism, presence of metastases, radiotherapy, anthelmintic use, palliative chemotherapy and discomfort in the lower limbs. The results show that PSTP is a common finding in cancer patients during cancer chemotherapy.


Subject(s)
Humans , Male , Female , Antineoplastic Agents/adverse effects , Sensory Receptor Cells , Drug-Related Side Effects and Adverse Reactions , Perception , Touch , Nursing Diagnosis/classification , Validation Studies as Topic
20.
Protein & Cell ; (12): 443-452, 2015.
Article in English | WPRIM | ID: wpr-757581

ABSTRACT

Voltage-gated sodium channels (VGSCs) in primary sensory neurons play a key role in transmitting pain signals to the central nervous system. BmK I, a site-3 sodium channel-specific toxin from scorpion Buthus martensi Karsch, induces pain behaviors in rats. However, the subtypes of VGSCs targeted by BmK I were not entirely clear. We therefore investigated the effects of BmK I on the current amplitude, gating and kinetic properties of Nav1.8, which is associated with neuronal hyperexcitability in DRG neurons. It was found that BmK I dose-dependently increased Nav1.8 current in small-sized (<25 μm) acutely dissociated DRG neurons, which correlated with its inhibition on both fast and slow inactivation. Moreover, voltage-dependent activation and steady-state inactivation curves of Nav1.8 were shifted in a hyperpolarized direction. Thus, BmK I reduced the threshold of neuronal excitability and increased action potential firing in DRG neurons. In conclusion, our data clearly demonstrated that BmK I modulated Nav1.8 remarkably, suggesting BmK I as a valuable probe for studying Nav1.8. And Nav1.8 is an important target related to BmK I-evoked pain.


Subject(s)
Aniline Compounds , Pharmacology , Animals , Cell Size , Cells, Cultured , Electrophysiological Phenomena , Furans , Pharmacology , Ganglia, Spinal , Cell Biology , Kinetics , Male , Metabolism , Rats , Rats, Sprague-Dawley , Scorpion Venoms , Pharmacology , Scorpions , Sensory Receptor Cells , Metabolism , Physiology , Sodium Channel Blockers , Pharmacology , Voltage-Gated Sodium Channel Agonists , Pharmacology
SELECTION OF CITATIONS
SEARCH DETAIL