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Electron. j. biotechnol ; 46: 30-37, jul. 2020. tab, graf
Article in English | LILACS | ID: biblio-1223233


BACKGROUND: The effects of dietary nutrition on tail fat deposition and the correlation between production performance and the Hh signaling pathway and OXCT1 were investigated in fat-tailed sheep. Tan sheep were fed different nutritional diets and the variances in tail length, width, thickness and tail weight as well as the mRNA expression of fat-related genes (C/EBPα, FAS, LPL, and HSL) were determined in the tail fat of sheep at three different growth stages based on their body weight. Furthermore, the correlations between tail phenotypes and the Hedgehog (Hh) signaling pathway components (IHH, PTCH1, SMO, and GLI1) and OXCT1 were investigated. RESULTS: C/EBPα, FAS, LPL, and HSL were expressed with differences in tail fat of sheep fed different nutritional diets at three different growth stages. The results of the two-way ANOVA showed the significant effect of nutrition, stage, and interaction on gene expression, except the between C/EBPα and growth stage. C/EBPα, FAS, and LPL were considerably correlated with the tail phenotypes. Furthermore, the results of the correlation analysis demonstrated a close relationship between the tail phenotypes and Hh signaling pathway and OXCT1. CONCLUSIONS: The present study demonstrated the gene-level role of dietary nutrition in promoting tail fat deposition and related tail fat-related genes. It provides a molecular basis by which nutritional balance and tail fat formation can be investigated and additional genes can be identified. The findings of the present study may help improve the production efficiency of fat-tailed sheep and identify crucial genes associated with tail fat deposition.

Animals , Tail/metabolism , Sheep/genetics , Adipose Tissue , Diet , Phenotype , RNA, Messenger , Coenzyme A-Transferases , Gene Expression , Body Fat Distribution , Adipogenesis , Lipogenesis/genetics , Hedgehog Proteins/genetics , Real-Time Polymerase Chain Reaction
Pesqui. vet. bras ; 38(4): 624-628, abr. 2018. graf
Article in English | LILACS, VETINDEX | ID: biblio-955384


Scrapie is a transmissible spongiform encephalopathy (TSE) that affects sheep and goats and results from accumulation of the abnormal isoform of a prion protein in the central nervous system. Resistance or susceptibility to the disease is dependent on several factors, including the strain of infecting agent, the degree of exposure, and the presence of single nucleotide polymorphisms (SNPs) in the prion protein gene. The most important polymorphisms are present in codons 136, 154, and 171. SNPs have also been identified in other codons, such as 118, 127, 141, 142, and 143. The objective of this study was to investigate the genotypic profile of Santa Ines (n=94) and Dorset (n=69) sheep and identify polymorphisms in the prion protein gene using real-time PCR techniques and sequencing. We analyzed SNPs in 10 different codons (127, 136, 138, 140, 141, 142, 143, 154, 171, and 172) in Santa Ines sheep. Classification of the flock into risk groups associated with scrapie revealed that approximately 68% of the Santa Ines herd was considered at moderate risk (group 3), and the most frequent haplotype was ARQ/ARQ (47.8%). For Dorset sheep, 42% of the herd was considered at moderate risk (group 3), 40% at low risk (group 2), and 12% at very low risk (group 1). These findings improve our understanding of the genotype breed and further highlight the importance of genotyping and identification of polymorphisms in Brazilian herds to assess their effects on potential infections upon exposure to the sheep prion.(AU)

Scrapie é uma encefalopatia espongiforme transmissível que afeta ovinos e caprinos, resultante do acúmulo de uma isoforma anormal da proteína priônica no sistema nervoso central. A resistência ou susceptibilidade está relacionada a diversos fatores, tais como, a cepa do agente infectante, o grau de exposição e o polimorfismo de nucleotídeo único (SNPs) do gene da proteína priônica. Os principais polimorfismos estão presentes nos códons 136, 154 e 171. SNPs também são identificadas em outros códons, tais como, 118, 127, 141, 142, e 143. O objetivo do trabalho foi descrever o perfil genotípico de um rebanho da raça Santa Inês (n=94) e um rebanho da raça Dorset (n=89) para identificar potenciais polimorfismos através da técnica de PCR em tempo real e sequenciamento. Os achados no rebanho Santa Inês indicaram a presença de polimorfismos de nucleotídeos únicos em 10 códons diferentes (127, 136, 138, 140, 141, 142, 143, 154, 171 e 172). A classificação do rebanho, quanto aos grupos de risco associados ao scrapie, relevaram que aproximadamente 68% dos ovinos foram considerados do grupo de risco moderado (grupo 3), onde o haplótipo mais frequente foi ARQ/ARQ (47,8%). Para os ovinos da raça Dorset, 42% do rebanho foi considerado do grupo de risco moderado (grupo 3), 40% do grupo de risco baixo (grupo 2) e 12% do grupo de risco muito baixo. Os dados encontrados contribuem para o conhecimento do genótipo das raças, destacando a importância de trabalhos que relatam os polimorfismos genéticos para a identificação de rebanhos brasileiros, bem como o seu impacto a infecções com exposição ao príon ovino.(AU)

Animals , Scrapie , Sheep/genetics , Polymorphism, Single Nucleotide/genetics , Prion Proteins/analysis
Arq. bras. med. vet. zootec. (Online) ; 69(4): 1047-1053, jul.-ago. 2017. tab, ilus
Article in English | LILACS, VETINDEX | ID: biblio-876943


Polymorphisms in the BMP-15 gene related to Galway (FecXG) and Inverdale (FecXI) and in the BMPR-1B gene known as Booroola (FecB) mutations were investigated using the Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) method, on sheep from the breeds Santa Inês (n= 574) and Morada Nova (n=282). DNA was extracted and amplified through PCR with specific primers that introduced a restriction site in association with the mutation. The PCR products were submitted to endonucleases. The experiment found no FecXG and FecXI mutations. Six samples of animals with multiple offspring/birth history presented polymorphism for FecB similar to control samples, but this pattern was not confirmed by nucleotide sequencing. Although the absence of these mutations in the studied breeds, other factors related to prolificacy should be investigated to explain the inherent prolificity mechanisms.(AU)

Polimorfismos Galway (Fec XG ) e Inverdale (Fec XI), relacionados ao gene BMP-15, e Booroola (FecB), localizado no gene BMPR-1B, foram investigados usando-se a técnica de reação em cadeia da polimerase - polimorfismo de comprimento de fragmentos de restrição (PCR-RFLP), em ovelhas Santa Inês (n= 574) e Morada Nova (n=282). O DNA foi extraído e amplificado por PCR com iniciadores específicos, que introduziram um sítio de restrição associado à mutação, em seguida os amplicons foram submetidos à ação de endonucleases. Não foram observadas as mutações Fec XG e Fec XI nas amostras estudadas. Amostras de seis animais com histórico de partos gemelares apresentaram polimorfismo para FecB semelhantes às amostras controle, mas esse padrão não foi confirmado pelo sequenciamento de nucleotídeos. Apesar da ausência dessas mutações nos animais das raças estudadas, outros fatores relacionados à prolificidade devem ser pesquisados para explicar os mecanismos da alta prolificidade desses animais.(AU)

Animals , Polymorphism, Genetic , Sheep/genetics , Polymerase Chain Reaction/veterinary , Polymorphism, Restriction Fragment Length
Arq. bras. med. vet. zootec ; 69(2): 429-436, mar.-abr. 2017. tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-833949


Profitability of sheep production depends on the reproductive response of ewes and growth of their lambs, which can be improved through the ram genotype. The aim of this study was to evaluate the reproductive response of Blackbelly (Bb), Pelibuey (Pb), Katahdin (Kat) and Dorper (Dor) ewes crossed with Damara (Dam) and Dor rams and the growth of their offspring. To measure percentage of single and multiple lambings (MLP), 234 Bb, Pb, Kat and Dor ewes were used. To measure lamb growth, the offspring of 86 Bb x Pb (BbPb) ewes and 73 Kat x Dor (KatDOR) ewes were used. Four Dor x BbPb, six Dam x BbPb and six Dam x KatDor lambs were slaughtered for carcass assessment. MLP was analyzed with the CATMOD procedure considering the factors age and breed. Lamb growth data were analyzed with the MIXED procedure, and those of carcass characteristics with the GLM procedure of SAS, using weight at slaughter as covariable. MLP was higher (P<0.05) in Bb than in Dor. The Dor x BbPb lambs were heavier (P<0.05) at birth than Dam x BbPb. Of the lambs from single and multiple births, the KatDor lambs were heavier (P<0.05) at birth than BbPb lambs. The female lambs of Dor rams had higher (P<0.05) weight gain after weaning than those of Dam rams. Carcass dressing, subcutaneous fat, and primal cut yield were not different (P>0.05) by effect of ram breed. It is concluded that Bb ewes are more prolific than Dor ewes; male lambs of Dam rams had post-weaning growth response and carcass yield similar to those of Dor rams, although the female lambs of Dor rams were heavier than those of Dam rams.(AU)

Rentabilidade da produção de ovinos depende da resposta reprodutiva de ovelhas e do crescimento de seus cordeiros, o que pode ser melhorado por meio do genótipo masculino. O objetivo deste estudo foi avaliar o desempenho reprodutivo de ovinos Blackbelly (Bb), Pelibuey (Pb), Katadin (Kat) e Dorper (Dor) e o crescimento de cordeiros com carneiros Damara (Dam) e Dor. Para medir a porcentagem de partos simples e múltiplas (MLP), foram utilizados 234 ovelhas Bb, PB, Kat e Dor. Para medir o crescimento dos cordeiros, foram utilizadas 86 ovelhas Bb x Pb (BbPb) e 73 Kat x Dor (KatDOR). Quatro cordeiros Dor x BbPb, seis Dam x BbPb e seis Dam x KatDor foram sacrificados para avaliação de carcaça. MLP foi analisada com o procedimento CATMOD, considerando-se fatores como idade e raça. Dados de crescimento do cordeiro foram analisados com o procedimento MIXED, e as de características de carcaça com o procedimento GLM do SAS, utilizando-se peso ao sacrifício como covariável. MLP foi maior (P<0.05) em Bb do que em Dor. Os cordeiros Dor x BbPb foram mais pesados (P<0.05) no nascimento do que Dam x BbPb. Em partos simples e múltiplos, os cordeiros de ovelhas KatDor foram mais pesados (P<0.05) no momento do nascimento de cordeiros BbPb. As cordeiras de carneiros Dor apresentaram maior (P<0.05) ganho de peso após o desmame do que as de carneiros Dam. O rendimento de carcaça, a espessura da gordura subcutânea e o rendimento de corte primal não foram diferentes por efeito da raça de carneiro. Conclui-se que as ovelhas Bb são mais prolíficas do que as ovelhas Dor; cordeiros de carneiros Damara tiveram resposta de crescimento pós-desmame e rendimento de carcaça semelhantes aos dos carneiros Dor, embora Dorper cordeiras fossem mais pesadas do que as de Damara.(AU)

Animals , Litter Size/genetics , Reproduction/genetics , Sheep/genetics , Sheep/growth & development , Weaning , Weight Gain , Reproductive Physiological Phenomena/genetics
Braz. j. med. biol. res ; 50(11): e6177, 2017. tab, graf
Article in English | LILACS | ID: biblio-888945


The human calcium- and integrin-binding protein (CIB) family is composed of CIB1, CIB2, CIB3, and CIB4 proteins and the CIB4 gene affects fertility. Kermani sheep is one of the most important breeds of Iranian sheep breeds. The aim of this study was to analyze for the first time molecular characteristics of the CIB4 gene and protein in Kermani sheep. Different tissues were collected from the Kermani sheep and real time PCR was performed. The PCR products were sequenced, comparative analyses of the nucleotide sequences were performed, a phylogenetic tree was constructed, and different characteristics of CIB4 proteins were predicted. Real time PCR results showed that the CIB4 gene is expressed only in testis of Kermani sheep. The cDNA nucleotide sequence was identical with small tail Han sheep, cattle, goat, camel, horse, dog, mouse and human, respectively 100, 99, 99, 98, 98, 96, 96, and 96%. Hence, it can be suggested that the CIB4 gene plays a role in male fertility. Based on the phylogenetic analysis, sheep CIB4 gene has a close relationship with goat and cattle first, and then with camel and whale. Although we demonstrated that CIB4 is a testis-specific gene, expressed only in the testis and it interacts with other proteins, the mechanisms by which CIB4 expression is regulated need to be elucidated.

Animals , Male , Female , Calcium-Binding Proteins/analysis , Calcium-Binding Proteins/genetics , Sheep/genetics , Amino Acid Sequence , Base Sequence , Electrophoresis/veterinary , Phylogeny , Real-Time Polymerase Chain Reaction/veterinary , Reference Values
Pesqui. vet. bras ; 36(5): 383-388, tab, graf
Article in English | LILACS | ID: lil-787588


This study was designed to assess the influence of genetic resistance against brachiaria poisoning in sheep. Two groups of sheep, one identified as susceptible (formed by two ewes and one ram) and the other as resistant against brachiaria poisoning (formed by three ewes and one ram) were selected. Sheep considered susceptible were those that presented clinical signs of brachiaria poisoning at any time of their life; resistant sheep were those that even raised on Brachiaria spp. pastures, did not developed any sign of the poisoning during their life. The offspring of the two flocks (15 lambs from the sensitive flock and 9 lambs from the resistant flock) were placed into brachiaria pasture (initially Brachiaria decumbens and B. brizantha,and only B. decumbens after weaning) and followed up during two years (2013-2014). The determination of protodioscin levels in B. decumbens pasture was performed only in 2014 and revealed significant amounts of the toxic principle. Eleven lambs of the susceptible group were affected to some degree of brachiaria poisoning and six died; no lamb of the resistant group was affected. Clinical signs consisted of varying degrees of subcutaneous edema of the face and, erythema and loss of hair of the ears, crusts on the skin of ears, around the eyes and on planum nasale, scar deformation of the ears, and bilateral ocular discharge; affected lambs also sought for shadowy shelters and they were poor doers. Several sheep recovered from the condition and then relapsed. Necropsy findings in six lambs included pale mucous membranes, emaciation, dermatitis, scar deformation of the ears, large yellow livers with marked lobular pattern, and moderate infestation by Haemonchus contortus...

Este estudo avaliou a resistência genética na ocorrência de intoxicação por braquiária em ovinos. Foram selecionados dois grupos de ovinos, um identificado como suscetível (formado por duas ovelhas e um carneiro) e o outro como resistente (formado por três ovelhas e um carneiro). Foram considerados suscetíveis ovinos que apresentaram sinais de intoxicação por Brachiaria spp. em algum ponto de suas vidas e resistentes aqueles ovinos que, mesmo criados em pastagem de braquiária, nunca desenvolveram qualquer sinal da intoxicação. A progênie desses dois grupos (15 cordeiros do grupo suscetível e 9 no grupo resistente) foi colocada numa pastagem de braquiária (inicialmente Brachiaria decumbens e B. brizantha e, após o desmame, apenas B. decumbens) e acompanhada durante dois anos (2013-2014). A determinação dos níveis de protodioscina em B. decumbens foi realizada apenas em 2014 e foram encontradas quantidades significativas do princípio tóxico. Onze cordeiros do grupo suscetível foram afetados por algum grau de intoxicação por braquiária; nenhum cordeiro do grupo resistente foi afetado. Os sinais clínicos consistiam de graus variáveis de edema subcutâneo da face e eritema e alopecia da pele das orelhas, crostas na pele das orelhas e ao redor dos olhos e no plano nasal, retração cicatricial das orelhas, fotofobia e corrimento ocular bilateral. Três cordeiros apresentaram desenvolvimento retardado. Vários cordeiros se recuperaram da condição, mas posteriormente quando foram colocados na pastagem apresentaram recidivas. Achados de necropsia em seis cordeiros incluíam mucosas pálidas, pobre condição corporal, dermatite, deformação cicatricial das orelhas, fígado aumentado de volume, amarelo e com padrão lobular evidenciado e graus moderados de infestação por Haemonchus contortus...

Animals , Brachiaria/toxicity , Plant Poisoning/genetics , Plant Poisoning/immunology , Sheep/genetics , Sheep/immunology , Saponins/toxicity , Plants, Toxic
Braz. j. biol ; 76(1): 205-208, Feb. 2016. tab, graf
Article in English | LILACS | ID: lil-774521


Abstract The objective of this study was to evaluate the frequency of C242T polymorphism on the aromatase gene and the allelic and genotypic frequency of these variants in sheep belonging to four breed groups. Blood samples were collected from 187 animals of four breed groups: Dorper, Santa Inês, Texel and White Dorper, originated from herds in the region of Maringá/PR, Brazil. The genomic DNA was extracted using alkaline extraction, with subsequent amplification of the fragments via PCR with specific primer. The samples resulting from amplification were subjected to digestion process using the Dpn II restriction enzyme and to polyacrylamide gel electrophoresis 10.0% and stained with silver nitrate. Three distinct genotypes were observed: homozygous (CC), heterozygous (CT) and homozygous for no cut (TT). The resulting data were analyzed using the POPGENE software with 5% significance. Genotypic frequencies among the breed groups were: Texel (CC - 0.426; CT - 0.511; TT - 0.064), Dorper (CC - 0.073; CT - 0.732; TT - 0.439), White Dorper (CC - 0.021; CT - 0.255; TT - 0.723) and Santa Inês (CC - 0.115; CT - 0.462; TT - 0.423).

Resumo O objetivo deste trabalho foi avaliar as frequências alélicas e genotípicas do polimorfismo do C242T no gene da aromatase em ovinos pertencentes a quatro grupos raciais. Foram coletadas amostras de sangue de 187 animais de quatro grupos raciais: Dorper, Santa Inês, Texel e White Dorper, provenientes de rebanhos da região de Maringá, PR - BR. O DNA genômico foi extraído utilizando o método de extração alcalina, com posterior amplificação dos fragmentos via PCR com primer específico. As amostras resultantes da amplificação foram submetidas ao processo de digestão com auxilio da enzima restrição Dpn II e submetido à eletroforese em gel de poliacrilamida de 10,0% e corado nitrato de prata. Foram observados três genótipos distintos: Homozigoto (CC), heterozigoto (CT) e homozigoto para não corte (TT). Os dados resultantes foram analisados utilizando o software POPGENE com significância de 5%. As frequências genotípicas entre os grupos raciais foram: Texel (CC - 0,426; CT - 0,511; TT - 0,064), Dorper (CC - 0,073; CT - 0,732; TT - 0,439), White Dorper (CC - 0,021; CT - 0,255; TT - 0,723) e Santa Inês (CC - 0,115; CT - 0,462; TT - 0,423).

Animals , Aromatase/genetics , Gene Frequency , Polymorphism, Genetic , Sheep/genetics , Aromatase/metabolism , Genotype , Sheep/metabolism
Pesqui. vet. bras ; 36(2): 73-76, fev. 2016. tab, graf
Article in English | LILACS | ID: lil-777392


Dermatosparaxis is an autosomal recessive disorder of connective tissue; the disorder is clinically characterized by skin fragility and hyperextensibility. Dermatosparaxis in White Dorper sheep is caused by a single nucleotide polymorphism (SNP) (c.421G>T) in the ADAM metalloproteinase with thrombospondin type 1 motif, 2 (ADAMTS2) gene. The aim of this study was to investigate the prevalence of this SNP in a White Dorper herd in São Paulo state, Brazil. In this study, we collected blood DNA samples from 303 White Dorper sheep and performed polymerase chain reaction to amplify the SNP region. The samples were sequenced to determine the presence of the SNP in the ADAMTS2 gene. The SNP prevalence in the studied population was 15.5%; this finding indicates that more effective control measures should be used to prevent the inheritance of SNP c.421G>T in the ADAMTS2 gene in Brazilian White Dorper herds.

A dermatosparaxia é uma doença autossômica recessiva do tecido conjuntivo, clinicamente caracaterizada pela fragilidade e hiperextensibilidade da pele. A dermatosparaxia em ovinos White Dorper é causada pelo polimorfismo de base única (SNP) c.421G>T no gene ADAM metalopeptidase com trombospondina tipo 1 motif, 2 (ADAMTS2). O objetivo deste estudo foi investigar a prevalência deste SNP em ovinos White Dorper no estado de São Paulo, Brasil. Foram coletadas amostras de sangue de 303 ovinos White Dorper. O DNA foi purificado destas amostras sanguíneas e utilizado em uma reação em cadeia da polimerase (PCR) para amplificação da região do gene contendo SNP c.421G>T. Os produtos das PCR foram sequenciados para determinar o genótipo dos animais. A prevalência do SNP na população estudada foi de 15,5%, estes achados indicam que medidas de controle efetivas devem ser utilizadas para prevenir a disseminação deste SNP no rebanho brasileiro de White Dorper.

Animals , Skin Abnormalities/prevention & control , Asthenia/veterinary , Cutis Laxa/veterinary , Sheep/genetics , Polymorphism, Single Nucleotide/genetics , Skin Abnormalities/veterinary , DNA , Polymerase Chain Reaction/veterinary , Ehlers-Danlos Syndrome/veterinary
Arq. bras. med. vet. zootec ; 67(6): 1646-1652, nov.-dez. 2015. tab
Article in Portuguese | LILACS | ID: lil-768163


Foi estudado o desempenho reprodutivo de 482 ovelhas multíparas (3/4 Texel x 1/4 Corriedale: TXC, 1/2 Romney Marsh x 1/2 Corriedale: RMC, 3/4 Poll Dorset x 1/4 Corriedale: PDC, 1/2 Finnish Landrace x 1/2 Merino Australiano: FLMA e Merino Australiano puras: MA), as características do crescimento de 585 cordeiros provenientes de carneiros Dorper e ovelhas: TXC (n = 68); RMC (n = 64); PDC (n = 68); FLMA (n = 183) e MA (n = 202) e os quilogramas de carne de cordeiros produzidos/kg de ovelha colocadas em cobertura (como indicador de eficiência), pastoreando campo natural e pastagens cultivadas. As ovelhas FLMA e TXC apresentaram as maiores taxas de parição (167,89 e 174,36%, respectivamente) em relação às demais cruzas, as quais não diferiram entre si (121,43 e 125,49%, PDC e RMC, respectivamente; P>0,05), porém foram superiores as MA (88,99%), sendo esse resultado uma consequência da maior quantidade de cordeiros nascidos/ovelha parida, que influenciou mais do que sua maior taxa de fertilidade. O genótipo da ovelha também afetou (P<0,0001) o crescimento dos cordeiros até o momento da sua venda com 41,6 ±6,7kg aos 206±7,25 dias de idade. Os cordeiros filhos das ovelhas TXC, PDC e RMC apresentaram maiores ganhos de peso diário que os filhos de FLMA, e esses maiores do que os MA. No entanto, de todas as cruzas avaliadas, somente a FLMA foi mais eficiente que a MA.

The reproductive performance of 482 multiparous ewes, with growth characteristics of 585 lambs from Dorper rams and ewes: 3/4 Texel x 1/4 Corriedale (TXC, n = 68); 1/2 Romney Marsh x 1/2 Corriedale (RMC, n = 64); 3/4 Poll Dorset x 1/4 Corriedale (PDC, n = 68); 1/2 Finnish Landrace x Australian Merino (FLAM, n = 183) and Australian Merino pure ewes (AM, n = 202) and kg of lamb produced/kg of sheep used in the service (as an indicator of efficiency), grazing natural and sown pastures, were studied. The TXC and FLAM sheep had the highest lambing percentage (167.89 and 174.36%, respectively) compared to the rest of the other crosses, and did not differ from each other (121.43 and 125.49%, PDC and RMC respectively, P>0.05), but were higher than AM (88.99%), due to the greater number of lambs born / ewe calving rather than their higher fertility. The type of ewe also affected (P<0.0001) lamb growth until its sale at 41.6±6.7 kg to 206±7.25 days of age, showing mothers of TXC lambs, PDC and RMC had higher daily gains than the FLAM and these more than those of MA. However, in all crosses tested, only FLAM was more efficient than MA.

Animals , Crosses, Genetic , Hybrid Vigor , Sheep/genetics , Selective Breeding , Reproductive Techniques/veterinary
Arq. bras. med. vet. zootec ; 67(4): 1119-1124, July-Aug. 2015. tab
Article in Portuguese | LILACS | ID: lil-759243


O objetivo do trabalho foi estimar os parâmetros genéticos das características de crescimento da raça Suffolk, a fim de fornecer subsídio para a definição de estratégias de seleção para programas de melhoramento genético. Os dados analisados, coletados entre os anos de 2007 a 2009, são referentes a ovinos da raça Suffolk oriundos de uma propriedade localizada no Estado de São Paulo, Brasil, participante do programa de melhoramento genético Ovigol, conduzido pela empresa Aries Reprodução e Melhoramento Genético Ovino Ltda. em parceria com a empresa AbacusBio Limited, da Nova Zelândia. As características avaliadas foram: peso ao nascer (PN), ganho de peso pré-desmame (GPP) e peso ao desmame (PD), com número de registro de 1.039, 636 e 649 animais, respectivamente. Para análise estatística, utilizou-se o procedimento GLM do programa computacional SAS; para a consistência do pedigree e para as estimativas dos parâmetros genéticos, os programas computacionais utilizados foram Relax2: pedigree analysis program e o WOMBAT, respectivamente. O modelo linear geral incluiu sexo, grupo de contemporâneos (tipo de parto e ano de nascimento), covariável idade ao desmame (GPP e PD) com efeito quadrático, efeito genético aditivo direto, efeito de ambiente permanente materno e efeito residual. As herdabilidades para PN, GPP e PD foram 0,06, 0,42 e 0,37, respectivamente. As correlações genéticas entre PN e GPP, PN e PD e GPP e PD foram -0,10, -0,03 e 0,97, respectivamente. Na população estudada, as características GPP e PD, apresentam altas respostas à seleção, ao contrário do PN. As características PN e GPP são suficientes para compor um índice de seleção, sendo aconselhável o monitoramento do PN na fase inicial do programa.

The aim of this study was to estimate genetic parameters for growth of Suffolk, in order to provide a basis for the definition of selection strategies for breeding programs. The data analyzed are for Suffolk sheep, collected between years 2007 to 2009, from a property located in the State of São Paulo - Brazil, a participant in the Ovigol breeding program conducted by the company (Aries Reproduction and Breeding Sheep Ltd.) in partnership with (AbacusBio) New Zealand Limited. The evaluated characteristics were: birth weight (PN), weight gain pre-weaning (GPP) and weaning weight (PD), with registration number 1039, 636 and 649 respectively. For statistical analysis we used the GLM procedure of SAS software, for the consistency of pedigree and genetic parameter estimates software Relax2: pedigree analysis program and WOMBAT, respectively, were used. The general linear model included sex, contemporary group (type of birth and year of birth), covariate age at weaning (GPP and PD) with a quadratic effect, direct genetic effect, maternal permanent environmental effect and residual effect. Heritability estimates for PN, PD and GPP were 0.06, 0.42 and 0.37, respectively. Genetic correlations between PN and GPP, and PN and PD and GPP were -0.10, -0.03 and 0.97, respectively. In the study population characteristics GPP and PD have a high response to selection, unlike the PN. The GPP and PN characteristics are enough to make a selection index, it is advisable to monitor the PN in the initial phase of the program.

Animals , Genetic Enhancement , Genetic Fitness , Sheep/growth & development , Sheep/genetics , Models, Genetic , Chromosome Mapping/veterinary
Electron. j. biotechnol ; 18(2): 116-121, Mar. 2015. graf, tab
Article in English | LILACS | ID: lil-745579


Background In sheep breeding, there are situations where relationships recorded at the farm among pedigrees such as parent-offspring, full-sibs or half-sibs need to be tested. A panel of 28 microsatellite (MST) markers was tested to provide accurate pedigree information and resolve the common problem of significant error in pedigree records in Merino sheep. Three different flocks of Australian Merino sheep were investigated. A private farm flock represents a flock with no record availability. Two other flocks were maintained under good managements of full keeping records and being selected for high and low parasite resistances. Results In the studied panel, eight MSTs provided an average of Polymorphic Information content (PIC) equal to 0.65 or more in order to be sufficient to make an accurate and successful DNA-based parentage analysis. The panel of twenty-eight MST loci was obviously sufficient for providing 100% accurate pedigree and genotyping data. DNA-based pedigree records were constructed and all significant pedigree record errors were eliminated. Conclusions These results were used for further study of population genetic parameters such as recombination and haplotyping which heavily based on pedigree information. Nevertheless MST based parentage testing is still available and affordable in most countries and for each farmer with reasonable cost in comparison with fast growing SNP based parentage technologies.

Animals , Paternity , Sheep/genetics , Microsatellite Repeats , Pedigree , Polymorphism, Genetic , DNA , Genetic Markers , Alleles , Genotype
Electron. j. biotechnol ; 18(2): 122-127, Mar. 2015. ilus, graf, tab
Article in English | LILACS | ID: lil-745580


Background The objective of this study was to investigate proliferator-activated receptor (PPARγ), fatty acid synthase (FAS) and hormone-sensitive lipase (HSL) mRNA and protein expression in fat tails of Tan sheep. Rams from different developmental stages (aged 3, 6, 9, 12, 15 and 18 months) were selected, and their tail measurements including length (L), width (W) and girth (G) were recorded. The mRNA and protein expressions of PPARγ, FAS and HSL were examined by quantitative real-time polymerase chain reaction (PCR) and Western blot. Results The tail measurements increased with age. We observed no significant differences (P > 0.05) of PPARγ mRNA expression between ages 9 and 15 months, and between 12 and 15 months; FAS mRNA expression levels at each developmental stage were observed significantly in Tan sheep (P < 0.05); HSL mRNA expression with no significant differences were only observed between 6 and 15 months (P > 0.05). Significant differences (P < 0.05) of PPARγ, FAS and HSL protein expressions at each developmental stage were observed in Tan sheep. Conclusion We observed that the mRNA expression patterns of PPARγ and FAS decreased first before they increased again and then this process repeated. Conversely, the mRNA expression patterns of HSL increased first before they decreased and then this process repeated. The protein expression patterns of PPARγ and FAS decreased first before they increased again and then this process repeated. Conversely, the protein expression pattern of HSL increased first before it decreased again and then increased again.

Animals , Sheep/growth & development , Sheep/genetics , Proteins/metabolism , Sterol Esterase/metabolism , PPAR gamma/metabolism , Fatty Acid Synthases/metabolism , Transcription Factors , RNA, Messenger , Blotting, Western , Sterol Esterase/genetics , PPAR gamma/genetics , Fatty Acid Synthases/genetics , Real-Time Polymerase Chain Reaction
Electron. j. biotechnol ; 17(6): 317-321, Nov. 2014. ilus, graf, tab
Article in English | LILACS | ID: lil-730264


Background The aim of the present study was to assess the genetic diversity of Pantaneiro sheep, using microsatellite markers, in order to assist maintenance and management plans, enhance mating systems and reduce the inbreeding rate. A total of 127 animals were genotyped at eight microsatellite loci. They belonged to populations from the Experimental Farm of the Universidade Federal da Grande Dourados (UFGD) (Dourados/MS/Brazil) and Embrapa Pantanal (Corumbá/MS/Brazil). Results The population of Pantaneiro sheep from the UFGD exhibited a high mean number of alleles (11.13) and allelic richness (10.66). The polymorphic information content was highly informative in the locus studied, resulting in a mean value of 0.71. Observed heterozygosity was lower than expected for all molecular markers assessed. The analysis of molecular variance showed a differentiation rate of 5.2% between populations. Conclusions The results of the statistical parameters indicated that populations of Pantaneiro sheep require special attention on herd management, and it's further necessary to implement breeder exchange programs in order to preserve the genetic variability of these populations. Furthermore, the maintenance of those populations in their typical habitats is rather required to allow different responses from the herds to the interactions between genotype and environment.

Animals , Sheep/genetics , Microsatellite Repeats , Phylogeny , Polymorphism, Genetic , Genetic Variation , Brazil , Breeding , Adaptation, Physiological , Analysis of Variance , Heterozygote
Arq. bras. med. vet. zootec ; 66(4): 969-976, 08/2014. tab
Article in Portuguese | LILACS | ID: lil-722567


Avaliaram-se as características espermáticas de carneiros Dorper, Santa Inês e sem padrão racial definido, nos períodos chuvoso e seco. Após ser colhido por vagina artificial, o sêmen foi avaliado quanto ao volume, ao turbilhonamento, à motilidade, ao vigor, à morfologia e à concentração, congelado e armazenado em botijão criogênico. Depois de descongelado, foram avaliadas a cinemática espermática, a integridade da membrana plasmática, a integridade do acrossoma e a atividade mitocondrial. Vigor espermático, motilidade total, motilidade progressiva, velocidade em linha reta e defeitos maiores não diferiram entre os períodos chuvoso e seco, porém volume, turbilhonamento, linearidade, retilinearidade e frequência de batimentos de cauda foram mais baixos (P<0,05) no período seco; já concentração espermática e defeitos totais apresentaram valores mais baixos no período chuvoso. Valores de integridade do acrossoma e da membrana plasmática, bem como o potencial de membrana mitocondrial, foram mais baixos (P<0,05) no período seco. Conclui-se que os períodos chuvoso e seco influenciam na qualidade espermática de ovinos criados na região Meio-Norte do Brasil e que esses animais têm uma qualidade espermática superior no período chuvoso, quando, portanto, deve ocorrer a criopreservação. Também se observou que, em relação à qualidade espermática, o melhor grupo de carneiros foi o Santa Inês...

The sperm characteristics of Dorper, Santa Ines and undefined breed ram in the rainy and dry seasons was assessed. After collection with an artificial vagina, the semen was evaluated for volume, turbulence, motility, viability, morphology and concentration, frozen and stored in a cryogenic cylinder. After thawing kinematic sperm, plasma membrane integrity, acrosome integrity and mitochondrial activity were evaluated. Sperm vigor, total motility, progressive motility, straight line speed and larger line defects did not differ between the rainy and dry seasons, however, volume, turbulence, linearity, straightness and frequency of tail beats were lower (P<0.05 ) in the dry period, and sperm concentration and total defects showed lower values during the rainy season. Values for acrosome integrity and plasma membrane and mitochondrial membrane potential were lower (P<0.05) in the dry season. It is concluded that the wet and dry period influence sperm quality in ram raised in the Mid - North region of Brazil, with a higher sperm quality in the rainy season, thus suggesting cryopreservation for that period. Also, the better ram group regarding sperm quality, was the Santa Ines...

Animals , Sperm Count/veterinary , Dry Season , Sheep/genetics , Semen Preservation/veterinary , Rainy Season , Sperm Capacitation , Sperm Motility , Semen Analysis/veterinary , Biomechanical Phenomena , Cell Membrane , Mitochondria
Electron. j. biotechnol ; 17(4): 168-173, July 2014. ilus, graf, mapas, tab
Article in English | LILACS | ID: lil-719108


Background Genetic diversity of sheep in Jordan was investigated using microsatellite markers (MS). Six ovine and bovine MS located on chromosomes 2 and 6 of sheep genome were genotyped on 294 individual from ten geographical regions. Results The number of alleles per locus (A), the expected heterozygosity (He) and observed heterozygosity (Ho) were measured. Overall A, He and Ho were 12.67, 0.820 and 0.684, respectively. On the other hand, genetic distances undoubtedly revealed the expected degree of differentiation among the studied populations. The finding showed closeness of three populations from south (Maan, Showbak and Tafeilah) to each other. Populations from the middle regions of Jordan (Karak, Madaba, Amman, AzZarqa and Mafraq) were found to be in one cluster. Only two populations of the middle region were an exception: AlSalt and Dead Sea. Finally, sheep populations from Irbid were located in separated cluster. It was clear that the studied predefined populations were subdivided from four populations and would be most probably accounted as ancestral populations. These results indicate that number of population is less than the predefined population as ten based on geographical sampling areas. Conclusions The possible inference might be that geographical location, genetic migration, similar selection forces, and common ancestor account for population admixture and subdivision of Awassi sheep breed in Jordan. Finally, the present study sheds new light on the molecular and population genetics of Awassi sheep from different regions of Jordan and to utilize the possible findings for future management of genetic conservation under conditions of climate changes and crossbreeding policy.

Animals , Genetic Variation , Sheep/genetics , Minisatellite Repeats , Phylogeny , DNA/isolation & purification , Alleles , Genetics, Population , Genotype , Jordan
Pesqui. vet. bras ; 32(12): 1230-1238, Dec. 2012. ilus, tab
Article in Portuguese | LILACS | ID: lil-662553


Scrapie é uma doença infecciosa, neurodegenerativa fatal, causada pelo príon scrapie (PrPsc). Apresenta-se tanto na forma clássica em ovinos e caprinos geneticamente susceptíveis quanto na forma atípica em ovinos. A primeira notificação oficial do Brasil à Organização Mundial de Saúde Animal (OIE), um caso da forma clássica diagnosticado no Rio Grande do Sul ocorreu em 1985, mas a doença já havia sido diagnosticada no mesmo Estado em 1978. Este trabalho objetivou descrever dois surtos de Scrapie em ovinos em Mato Grosso do Sul (MS), Brasil e investigar, por meio de imuno-histoquímica (IHQ) a presença de PrPsc no Sistema Nervoso Central (SNC) de ovinos examinados entre 2003 e 2010. Na primeira parte observaram-se dois ovinos com sinais clínicos típicos de scrapie, detalhando-se os sinais neurológicos, dados epidemiológicos, histopatológicos e amostras teciduais em duplicata desses ovinos foram encaminhadas para realização de diagnóstico de Raiva e para diagnóstico IHQ para príon. Na segunda parte realizou-se levantamento de laudos de necropsia e diagnósticos histopatológicos de ovinos, no período de maio de 2003 a março de 2010. Amostras de sistema nervoso central de 51 casos foram selecionados, incluindo os dois já com diagnóstico de Scrapie mencionados acima; os tecido de todos esses ovinos foram submetidos à IHQ para detecção de proteína priônica. Os 49 ovinos avaliados apresentaram resultado negativo na IHQ para príon.

Scrapie is a fatal neurodegenerative infectious disease, caused by the scrapie prion (PrPsc), that can both in the as the classic form in genetically susceptible sheep and goats and in the atypical form in sheep. The first official notification of scrapie from Brazil was made to the World Organization for Animal Health (OIE) in 1985, in the state of Rio Grande do Sul, although the disease was first documented in this Brazilian state in 1978. The objective this paper was to describe two outbreaks of scrapie in sheep from Mato Grosso do Sul (MS), Brazil, and to investigate by immunohistochemistry (IHC) the presence of PrPsc in samples from the CNS of sheep examined during 2003 and 2010. The study was conducted in two stages; the first was the observation of two sheep with typical clinical signs of scrapie that underwent clinical examination with emphasis on neurological parameters, epidemiological data collection, necropsy and collection of samples in duplicate forwarded to the diagnosis of rabies, and for the IHC diagnosis of Transmissible Spongiform Encephalopathies. In the second part of the study, a survey was made the necropsy reviewing gross findings and histopathological diagnoses in sheep from May 2003 to March 2010. Samples of the central nervous system of fifty-one cases, including the two sheep mentioned above were subjected to IHC for detection of prion protein. The other 49 sheep, although displaying neurological-disease which should be included as scrapie differential diagnosis, had their tissues submitted to IHC resulting negative.

Animals , Prion Diseases/veterinary , Sheep/abnormalities , Sheep/genetics , Scrapie/diagnosis , Fluorescent Antibody Technique, Direct/veterinary , Diagnosis, Differential , Neurodegenerative Diseases/veterinary , Gait Disorders, Neurologic
Electron. j. biotechnol ; 15(2): 7-7, Mar. 2012. ilus, tab
Article in English | LILACS | ID: lil-640542


Arylalkylamine N-acetyltransferase (AA-NAT) is critical enzyme in Melatonin (MLT) biosynthesis for MLT regulating the animal seasonal breeding. In this study, DNA sequencing methods were applied to detect the polymorphisms of the AA-NAT gene in 179 Chinese sheep belonging to two non-seasonal reproduction breeds and two seasonal reproduction breeds. One mutation at exon 3 (NM_001009461:c.486A > G) was firstly described at the sheep AA-NAT locus. Hence, we described the SmaI PCR-RFLP method for detecting EX3 486A > G mutation, frequencies of the AA-NAT-G allele varied from 0.871 to 0.908 in two non-seasonal reproduction breeds and 0.517 to 0.578 in two seasonal reproduction breeds. The associations of SmaI polymorphism with estrus traits was analyzed in non-seasonal reproduction breeds sheep and seasonal reproduction breeds sheep, the significant statistical results were found between them, the GG genotype frequencies was higher in non-seasonal reproduction breeds (p < 0.001), while, the GA genotype frequencies was higher in seasonal reproduction breeds (p < 0.05). Hence, the EX3 486A > G mutation could facilitate association analysis and serve as a genetic marker for Chinese sheep breeding and genetics.

Animals , Arylalkylamine N-Acetyltransferase/genetics , Sheep/physiology , Sheep/genetics , Arylalkylamine N-Acetyltransferase/physiology , Polymerase Chain Reaction , Polymorphism, Genetic , Polymorphism, Restriction Fragment Length , Reproduction/physiology , Seasons , Statistical Analysis
Int. j. morphol ; 29(3): 954-959, Sept. 2011. ilus
Article in English | LILACS | ID: lil-608688


Most Chilean sheep stock comprises different degrees of crossbreeding over Corriedale breed. A common absorbent crossbreeding has been Australian Merino over Corriedale which, in many cases, has not been complete. The aim of this study was to evaluate the process of morphology differentiation and structural functionality of Corriedale ewes undergoing incomplete absorbent crossbreeding which was carried out in order to create an animal with a new morphology. A total of four hundred adult ewes were measured; two hundred belonging to the incomplete crossbreeding, and two hundred from the two original breeds (one hundred Corriedale and one hundred Australian Merino ewes). All measured ewes were randomly selected. Fourteen body measurements were recorded and nine body indexes were calculated for each ewe. Results show that a new biotype has been created from the absorbent crossbreeding of Corriedale by Australian Merino, which produced ewes with a clear morphological and structural functionality differentiation as compared to the two original breeds. The new body format shows morphostructural variability coefficients that are similar to those found on other formally recognized sheep breeds.

La mayor parte del stock ovino chileno comprende diferentes grados de cruzamiento con la raza Corriedale. Un cruzamiento absorbente común ha sido el de Merino Australiano sobre Corriedale, el cual, en muchos casos, no ha sido completo. En este estudio se evaluó el proceso de diferenciación morfológica y funcionalidad estructural de las ovejas Corriedale que fueron previamente sometidas a cruzamiento absorbente incompleto con el fin de crear un animal con una nueva morfología. Se midió un total de cuatrocientas ovejas adultas, doscientas provenientes del cruzamiento incompleto y doscientas de las dos razas originales (cien ejemplares de raza Corriedale y cien de Merino australiano). Todas las ovejas medidas fueron seleccionadas al azar. Para cada animal se registraron catorce mediciones corporales y se calcularon nueve índices. Los resultados de los análisis permiten concluir que el cruzamiento absorbente de Corriedale por Merino Australiano ha generado un nuevo biotipo ovino, con una diferenciación morfológica clara y diferente funcionalidad estructural al ser comparadas con las dos razas originales. Los coeficientes de variabilidad morfoestructural que presenta el nuevo formato corporal fueron similares a los de otras razas ovinas formalmente reconocidas.

Young Adult , Hybridization, Genetic , Sheep/anatomy & histology , Sheep/genetics , Crosses, Genetic , Morphogenesis
Int. j. morphol ; 29(1): 123-126, Mar. 2011. ilus
Article in English | LILACS | ID: lil-591962


To date, no ethnological study on the wool characteristics of the Spanish Aranese ovine breed has been published. Fifty three animals belonging to this breed are tested as fleece samples. Each sample is analyzed for fleece type and length, yield by isoalcohol scouring, fiber length for each kind of fiber, variation in fiber diameter, and proportions of non-medullated and medullated or kemp fibers. Fiber length appears shorter than that previously reported for the breed by other authors. Fleeces of the Aranese sheep breed can be described as square, simple, "semi-open" and short with a relatively high yield by isoalcohol scouring and low medullation levels, thus indicating good potential quality and greater suitability for potential processing than other similar meat breeds.

Hasta el momento, ningún estudio etnológico sobre las características de la lana de la variedad española ovina Aranesa ha sido publicado. Cincuenta y tres animales de esta raza han sido analizados a partir de muestras de su vellón. Cada muestra se analizó para el tipo de vellón y longitud, su rendimiento mediante isoalcohol desgrasado, longitud de las fibras para cada tipo de fibra, variación en el diámetro de la fibra, y las proporciones de fibras no meduladas y meduladas o fibras kemp. La longitud de las fibras parece más corta que la reportada para esta raza por otros autores. El vellón de la raza ovina Aranesa se puede describir como cuadrado, simple, "semi-abierto" y corto, con un rendimiento relativamente alto por desengrasado mediante isoalcohol y bajos niveles de medulación, lo que indica la buena calidad potencial y mayor oportunidad para el procesamiento potencial que otras razas similares de carne.

Animals , Male , Female , Adult , Wool/anatomy & histology , Wool/cytology , Wool/growth & development , Wool/pathology , Wool/ultrastructure , Sheep/anatomy & histology , Sheep/genetics
IJB-Iranian Journal of Biotechnology. 2011; 9 (2): 145-149
in English | IMEMR | ID: emr-108430


Calpastatin is an endogenous inhibitor of calpain [calcium-dependent cysteine protease]. Calpastatin activity is highly related to the rate of protein turnover and rate of meat tenderization. In order to characterize the structure of calpastatin in Iranian Afshari breed of sheep, intron 6 and partial exon 7 of the L domain were amplified and sequenced. A fragment of approximately 1.5 kb was identified. In this study, an Afshari calpastatin gene fragment that encoded L Domain amino acids was detected. Hence by detection of such conserved mutations, it is possible to use these polymorphisms in Marker-Assisted Selection [MAS]

Animals , Sheep/genetics , Sequence Analysis