Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 85
Filter
1.
Rev. Fac. Odontol. (B.Aires) ; 36(84): 21-26, 2021. tab
Article in Spanish | LILACS | ID: biblio-1363852

ABSTRACT

La terapia endodóntica tiene como uno de sus objetivos lograr la completa desinfección del sistema de conductos radiculares. Por esto, se deben seleccionar sustancias irrigantes que tengan la capacidad de eliminar todo el contenido de dicho sistema. La acción antimicrobiana es una de las características más importantes a tener en cuenta en la elección. El hipoclorito de sodio (NaOCl) tiene capacidad bactericida sobre muchos de los microorganismos de la flora endodóntica. El Enterococcus faecalis es una bacteria altamente resistente a antibacterianos que sobrevive en condiciones extremas. El ácido hipocloroso (HOCl) es una molécula derivada del NaOCl que ha demostrado tener alto poder bactericida sobre cepas patogénicas bucales. El objetivo de este trabajo fue evaluar y comparar la efectividad antimicrobiana in vitro del NaOCl 2.5% y el HOCl al 5% frente a Enterococcus faecalis. Una suspensión de Enterococcus faecalis (ATCC29212), de turbidez 0.5 en escala de McFarland, fue inoculada en varios tubos de ensayo, los cuales contenían cada antimicrobiano. Se dejaron actuar durante 1, 5 y 10 minutos para luego neutralizarlos e inclubarlos a 37º C en condiciones de capnofilia durante 48 hs. Todo el procedimiento se realizó por quintuplicado. Los resultados se midieron mediante recuento de UFC/ml. No se evidenció presencia de Enterococcus faecalis en las placas que contenían la solución de NaOCl al 2.5% como tampoco en aquellas que contenían HOCl al 5%. In vitro, el HOCl y el NaOCl en las concentraciones probadas, eliminaron completamente las cepas de Enterococcus faecalis (AU)


Subject(s)
Sodium Hypochlorite/therapeutic use , Enterococcus faecalis/drug effects , Hypochlorous Acid/therapeutic use , Anti-Bacterial Agents/therapeutic use , Root Canal Irrigants/therapeutic use , In Vitro Techniques , Colony Count, Microbial , Culture Media , Dental Pulp Cavity/microbiology
2.
Rev. cuba. estomatol ; 57(1): e2872, ene.-mar. 2020. tab
Article in Spanish | LILACS, CUMED | ID: biblio-1126486

ABSTRACT

RESUMEN Introducción: La microfiltración es uno de los problemas principales de todas las obturaciones a base de resina. Muchos estudios buscan optimizar un buen sellado marginal utilizando diversos acondicionadores, como el hipoclorito de sodio, que permite que el adhesivo penetre bien, al generar desproteinización, y el ácido fosfórico que permite remover el barro dentinario, y así de esta manera formar una capa híbrida al colocar el adhesivo. Objetivo: Evaluar el grado de sellado marginal de la resina Bulk Fill aplicando gel de hipoclorito de sodio 10 por ciento comparado con el gel de ácido fosfórico al 37 por ciento en el acondicionamiento dental in vitro. Métodos: El universo fue de 72 dientes con cavidades clase I; se separaron en 4 grupos por muestra de 18 dientes cada uno; después de obturar se realizó el termociclaje a 500 ciclos entre 5 °C a 55 °C; luego se sumergieron en azul de metileno 2 por ciento, durante 12 h y 24 h a temperatura de 37 °C. Finalmente se evaluó el sellado marginal en el estereomicroscopio, según el ISO / TS 11405: 2015, con los criterios siguientes: grado 0 (sin microfiltración), grado 1 (microfiltración hasta esmalte), grado 2 (microfiltración hasta dentina) y grado 3 (microfiltración hasta piso pulpar). Resultados: El acondicionamiento con ácido fosfórico 37 por ciento a las 12 h presentó grado 0 (55,6 por ciento) y grado 1 (33,3 por ciento), y a las 24 h, grado 1 (55,6 por ciento). Con respecto al hipoclorito de sodio 10 por ciento a las 12 h, grado 0 (44.4 por ciento) y a las 24 h predominó grado 1 (55,6 por ciento). Para la contrastación de hipótesis en muestras relacionadas se obtuvo en ácido fosfórico 37 por ciento e hipoclorito de sodio al 10 por ciento a las 12 h y 24 h un p= 0,052 y p= 0,584, respectivamente. Para comparaciones entre muestras independientes a las 12 h y 24 h se obtuvo p= 0,462 y p= 0,406, respectivamente. Conclusiones: El gel de hipoclorito de sodio al 10 por ciento presenta similar eficacia en el sellado marginal respecto al gel de ácido fosfórico al 37 por ciento, al utilizarlo como acondicionador dental(AU)


ABSTRACT Introduction: Microfiltration is one of the main problems of all resin sealings. Many studies aim to optimize marginal sealing using a variety of conditioners, such as sodium hypochlorite, which allows good penetration of the adhesive by generating deproteinization, and phosphoric acid, which allows removal of the dental smear layer, thus creating a hybrid layer when the adhesive is placed. Objective: Evaluate the marginal sealing degree of Bulk Fill resin applying 10 percent sodium hypochlorite gel versus 37 percent phosphoric acid gel in in vitro dental conditioning. Methods: The study universe was 72 teeth with class I cavities, divided into four groups of 18 teeth. Upon sealing, thermal cycling was performed at 500 cycles from 5 ºC to 55 ºC. Next the teeth were submerged in 2 percent methylene blue for 12 h and 24 h at a temperature of 37 ºC. Finally marginal sealing was evaluated in the stereo microscope according to ISO / TS 11405: 2015, using the following scale: grade 0 (no microfiltration), grade 1 (microfiltration as far as the enamel), grade 2 (microfiltration as far as the dentin), and grade 3 (microfiltration as far as the pulp floor). Results: Conditioning with 37 percent phosphoric acid was grade 0 (55.6 percent) and grade 1 (33.3 percent) at 12 h, and grade 1 (55.6 percent) at 24 h, whereas conditioning with 10 percent sodium hypochlorite was grade 0 (44.4%) at 12 h, and predominantly grade 1 (55.6 percent) at 24 h. Hypothesis contrast in related samples yielded 37 percent phosphoric acid and 10 percent sodium hypochlorite at 12 h and 24 h, p= 0.052 and p= 0.584, respectively. Comparison between independent samples at 12 h and 24 h yielded p= 0.462 and p= 0.406, respectively. Conclusions: The 10 percent sodium hypochlorite gel has similar marginal sealing effectiveness as the 37 percent phosphoric acid gel when used as dental conditioners(AU)


Subject(s)
Humans , Phosphoric Acids/adverse effects , Pit and Fissure Sealants/therapeutic use , Sodium Hypochlorite/therapeutic use , Microstraining/methods , Smear Layer/etiology
3.
J. appl. oral sci ; 28: e20190100, 2020. tab, graf
Article in English | LILACS, BBO | ID: biblio-1056594

ABSTRACT

Abstract Objective: This clinical study sought to evaluate the effectiveness of passive ultrasonic activation (PUA) in eliminating microorganisms in primary endodontic infection (PEI) after instrumentation of root canals using microbiological culture and checkerboard DNA-DNA hybridization. Methodology: Twenty root canals with PEI and apical periodontitis were selected. The root canals were instrumented and then randomly divided into 2 groups, according to the irrigation method: PUA and conventional needle irrigation (CNI). Microbiological samples were collected before instrumentation (S1), after instrumentation (S2) and after irrigation with 17% EDTA (S3). The samples were subjected to anaerobic culture technique and checkerboard DNA-DNA hybridization analysis. Results: A statistically significant difference was found between CNI (23.56%) and PUA (98.37%) regarding the median percentage values for culturable bacteria reduction (p<0.05). In the initial samples, the most frequently detected species was S. constellatus (50%), and after root canal treatment was E. faecalis (50%). Conclusion: Both treatments significantly decreased the number of bacterial species compared with the initial sample. However, no statistical difference in the total microbial load between PUA and CNI groups was detected. The number of cultivable anaerobic bacteria reduced significantly using PUA, and the bacterial composition and number of bacterial species after using either CNI or PUA was similar.


Subject(s)
Humans , Male , Female , Adolescent , Adult , Middle Aged , Periapical Periodontitis/therapy , Root Canal Therapy/instrumentation , Ultrasonic Therapy/instrumentation , Dental Pulp Cavity/microbiology , Root Canal Irrigants/therapeutic use , Root Canal Therapy/methods , Sodium Hypochlorite/therapeutic use , Bacteria/isolation & purification , Ultrasonic Therapy/methods , Colony Count, Microbial , DNA Probes , Linear Models , Analysis of Variance , Treatment Outcome , Cone-Beam Computed Tomography , Bacterial Load , Therapeutic Irrigation/instrumentation , Therapeutic Irrigation/methods
4.
Braz. oral res. (Online) ; 33: e021, 2019. tab, graf
Article in English | LILACS | ID: biblio-1001593

ABSTRACT

Abstract: This study investigated the effectiveness of XP-Endo Finisher (XPF) associated with XP-Endo Shaper (XPS) or Reciproc Blue (RB) files in reducing bacterial load in oval-shaped root canals (RC) during chemomechanical preparation (CMP) using 0.9% saline solution (NaCl) or 2.5% sodium hypochlorite (NaOCl). Eighty mandibular incisors with single oval-shaped RC were contaminated with Enterococcus faecalis. The teeth were randomly assigned to eight experimental groups (n = 10) according to the CMP, as follows: G1: XPS, G2: XPS + XPF, G3: RB, and G4: RB + XPF. CMP was performed with NaCl or NaOCl. The reduction of bacterial load was assessed by colony-forming unit count before (S1) and after (S2) CMP. Data normality was verified by using Shapiro-Wilk test. ANOVA, Tukey's test, and Bonferroni post-hoc test were used at a 5% significance level. Culturable bacteria were present in all S1 samples (p>0.05). All instrumentation techniques were effective in reducing bacterial load, irrespective of the irrigating solution (p < 0.05). With the use of NaCl, RB was more effective than XPS (p = 0.035). With the use of NaOCl, XPS and RB presented similar effectiveness (p = 0.779). XPF enhanced the bacterial reduction of both systems tested (p < 0.05). The use of NaOCl improved the CMP, irrespective of the instrumentation technique used (p < 0.05). In conclusion, XPS and RB files are effective in reducing bacterial levels in oval-shaped RC. The use of XPF as a method of agitation of the irrigating solution improved the cleaning efficiency of both file systems tested. Mechanical preparation performed with saline solution decreased culturable bacteria from the root canal, but antimicrobial substances such as NaOCl should be used to achieve a significantly better disinfection.


Subject(s)
Humans , Root Canal Preparation/instrumentation , Dental Instruments , Dental Pulp Cavity/anatomy & histology , Bacterial Load , Sodium Hypochlorite/therapeutic use , Materials Testing , Gram-Positive Bacterial Infections , Enterococcus faecalis/isolation & purification , Dental Pulp Cavity/microbiology , Disinfectants/therapeutic use , Saline Solution/therapeutic use , Incisor
5.
J. oral res. (Impresa) ; 7(7): 292-297, sept. 22, 2018. tab, graf
Article in English | LILACS | ID: biblio-1120999

ABSTRACT

Introduction: the aim of this study is to determine the current trends of irrigation during root canal therapy by specialists who are members of the Chilean Endodontic Society. materials and method: a survey (survey monkey -SurveyMonkey.com) was e-mailed to the 485 members of the Chilean Endodontic Society. the instrument was translated and adapted from the survey "irrigation trends among American Association of Endodontists members: a web-based survey" applied in the USA in 2012. participants answered a set of 16 questions that included irrigant selection, irrigant concentration, the adopted protocol, techniques or devices for irrigant activation. results: 99 percent of respondents use sodium hypochlorite as the main irrigant. data indicate that 74 percent of respondents use hypochlorite at a concentration of 5 percent. most respondents (94 percent) also include EDTA in their usual practice. In addition, 90 percent of respondents reported that they activate the irrigating agent, and 94 percent confirmed that they perform a final irrigation protocol. conclusion: the majority of respondents use sodium hypochlorite as the main irrigant at a concentration of 5 percent, use ethylenediaminetetraacetic acid (EDTA) as a smear removal agent, activate the irrigant, and perform a final irrigation protocol.


Subject(s)
Humans , Root Canal Irrigants/therapeutic use , Practice Patterns, Dentists'/statistics & numerical data , Endodontics/statistics & numerical data , Sodium Hypochlorite/administration & dosage , Sodium Hypochlorite/therapeutic use , Chile , Chlorhexidine/administration & dosage , Chlorhexidine/therapeutic use , Epidemiology, Descriptive , Surveys and Questionnaires , Smear Layer , Edetic Acid/administration & dosage , Edetic Acid/therapeutic use , Therapeutic Irrigation/methods
6.
Braz. oral res. (Online) ; 32: e16, 2018. tab, graf
Article in English | LILACS | ID: biblio-889502

ABSTRACT

Abstract The presence of residues within the root canal after post-space preparation can influence the bond strength between resin cement and root dentin when using fiberglass posts (FGPs). Currently, there is no consensus in the literature regarding what is the best solution for the removal of debris after post-space preparation. This systematic review involved "in vitro" studies to investigate if cleaning methods of the root canal after post-space preparation can increase the retention of FGPs evaluated by the push-out test. Searches were carried out in PubMed (MEDLINE) and Scopus databases up to July2017. English language studies published from 2007 to July 2017 were selected. 475 studies were found, and 9 were included in this review. Information from the 9 studies were collected regarding the number of samples, storage method after extraction, root canal preparation, method of post-space preparation, endodontic sealer, resin cement, cleaning methods after post-space and presence of irrigant activation. Five studies presented the best results for the association of sodium hypochlorite (NaOCl) and ethylenediamine tetra-acetic acid (EDTA), while in the other 4 studies, the solutions that showed improved retention of FGPs were photon-induced photoacoustic streaming (PIPS), Qmix, Sikko and EDTA. The results showed heterogeneity in all comparisons due to a high variety of information about cleaning methods, different concentrations, application time, type of adhesive system and resin cements used. In conclusion, this review suggests that the use of NaOCl/EDTA results in the retention of FGPs and may thus be recommended as a post-space cleaning method influencing the luting procedure.


Subject(s)
Humans , Dental Prosthesis Retention/methods , Glass , Post and Core Technique , Root Canal Irrigants/therapeutic use , Root Canal Preparation/methods , Dental Bonding/methods , Dental Pulp Cavity/drug effects , Dentin/drug effects , Edetic Acid/therapeutic use , Reproducibility of Results , Sodium Hypochlorite/therapeutic use , Treatment Outcome
7.
Postgrad. Med. J. Ghana ; 7(1): 1-7, 2018. ilus
Article in English | AIM | ID: biblio-1268719

ABSTRACT

Background: The majority of endodontic treatment in Ghana is provided by general dental practitioners due to the absence of specialists in endodontics. Sodium hypochlorite has been described as one of the commonly used irrigation solutions during endodontic treatment. However, there are no published reports on its use in Ghana. Aim: The study was to determine the proportion of Ghanaian dental practitioners who have used sodium hypochlorite for irrigation and the concentrations they usually use. Materials and methods: Self -administered questionnaires were mailed to dental practitioners in private clinics, government hospitals and clinics, teaching hospitals and training institutions across the country between December 2015 and March 2016. The collated data was analyzed using Microsoft Excel 2010 and SPSS 20.0.Results: The most commonly used root canal irrigant was 2.5% Sodium Hypochlorite (Milton®). This was routinely used by 31 (73.7%) of the respondents as root canal irrigant while normal saline solution was used regularly by only 6(15.8%) respondents. The various concentrations of sodium hypochlorite used were 0.5%, 2.5%,1%and 5.0%; with the following percentage-use respectively, 42.9%, 32.1%,21.4%and 6.1%. Three (10.7%) respondents had reported experiencing some complications with the use of sodium hypochlorite. Conclusion: Sodium hypochlorite is the most commonly used root canal irrigant by dental practitioners in Ghana. The concentrations usually used ranges between 0.5% and 5.0%


Subject(s)
Economics , Endodontics , Ghana , Root Canal Irrigants , Root Canal Irrigants/therapeutic use , Sodium Hypochlorite/chemistry , Sodium Hypochlorite/therapeutic use
8.
Rio de janeiro; s.n; 2016. 43 p. ilus.
Thesis in Portuguese | LILACS, BBO | ID: biblio-1017190

ABSTRACT

Diferentes manobras para promover agitação do hipoclorito de sódio têm sido propostas para incrementar a desinfecção do sistema de canais radiculares (SCR). O objetivo deste trabalho foi comparar, ex vivo, diferentes formas de agitação do NaOCl usado na irrigação final do SCR, avaliando a mais eficaz na redução de UFC/ml, a partir de biofilme de Enterococcus faecalis. Oitenta e cinco incisivos inferiores humanos extraídos, com canais ovais e obtidos do banco de dentes da UERJ foram acessados, contaminados com uma suspensão de E. faecalis (ATCC 29212) e incubados por 30 dias possibilitando crescimento bacteriano em biofilme. Os espécimes foram preparados com o instrumento Primary (25/08) do sistema Wave One TM. Foram formados cinco grupos contendo 15 dentes cada um, que foram irrigados com hipoclorito de sódio a 2,5% e, posteriormente, submetidos à agitação do irrigante como protocolo de irrigação final com o auxílio de diferentes instrumentos/ equipamentos, como a seguir: o EndoActivator ® (EA), o Easy Clean® (EC), a espiral de Lentulo (EL), a lima manual tipo K número 10 (LM) e o inserto ultrassônico Irrisonic - E1 (US). Foi formado o grupo controle positivo (n=5) o qual foi contaminado, sem a realização de nenhum procedimento. Foi formado também o grupo controle negativo (n=5) que não foi contaminado, mas seus espécimes foram irrigados com o NaOCl e submetidos a cada um dos protocolos de agitação da substância química auxiliar. As amostras microbiológicas foram coletadas usando cone de papel estéril antes da instrumentação (S1), após a instrumentação (S2) e após o protocolo final de irrigação (S3). Tais cones foram transferidos para tubos contendo meio BHI e incubados durante 48 horas. O crescimento bacteriano foi contado em unidades formadoras de colônia (UFC/ml). Os resultados foram submetidos aos testes Shapiro-Wilks, Wilcoxon e Kruskall-Wallis, para análise estatística ( P< 0.05) e demostraram que não houve diferença estatística significativa entre os grupos EA e EC, o grupo US quando comparado aos grupos EL e LM, foi significantemente mais eficiente na redução bacteriana. De acordo com os resultados do presente estudo, é possível afirmar que a agitação do NaOCl tem influência na atividade antibacteriana no SCR, e que o uso de ultrassom é mais eficiente quando comparado ao uso da espiral de lentulo e da lima manual.


Different maneuvers to promote agitation of sodium hypochlorite have been proposed to increase the disinfection of the root canal system. The objective of this study was to compare, ex vivo, different forms of agitation of chemical substance used in irrigation of the root canal system, evaluating the most effective in reducing or eliminating biofilms of Enterococcus faecalis. Eighty-five human mandibular incisors extracted with oval shape and obtained from UERJ teeth bank were accessed, contaminated with a suspension of E. faecalis (ATCC 29212) and incubated for 30 days allowing bacterial growth in biofilms. The specimens were prepared with the instrument Primary (25 / 0.08) Wave One TM system. Five groups were formed containing 15 teeth each, which were irrigated with sodium hypochlorite to 2.5% and then subjected to agitation of the irrigator as the final irrigation protocol with the help of different instruments / equipment, as follows: the EndoActivator ® (EA), Easy Clean® (EC), the Lentulo spiral (EL), the manual K file number 10 (LM) and the ultrasonic insert Irrisonic -E1 (US). The positive control group was formed (n=5), which was contaminated, without performing any procedure. It has also formed the negative control group containing (n=5), which was not infected but was irrigated with NaOCl and subjected to each of the agitation protocols of the irrigator. Microbiological samples were collected using sterile paper points before instrumentation (S1) after instrumentation (S2) and after the end of the irrigation protocol (S3). These points were transferred to Eppendorf containing BHI and incubated for 48 hours. Bacterial growth was counted in colony forming units (CFU / ml). The results were submitted to the Shapiro-Wilks test, Wilcoxon and Kruskal-Wallis test for statistical analysis and demonstrated a significant difference between the groups. US group compared to EL and LM groups was significantly more efficient in bacterial reduction. According to the results of this study it is clear that the agitation of NaOCl influences the antibacterial activity in the root canal system and that the use of ultrasonic is more efficient when compared to the use of lentullo and manual file.


Subject(s)
Humans , Root Canal Irrigants/therapeutic use , Sodium Hypochlorite/therapeutic use , Ultrasonics/methods , Root Canal Preparation/instrumentation , Enterococcus faecalis/drug effects , Statistics, Nonparametric , Root Canal Preparation/methods , Incisor
9.
Rev. ciênc. farm. básica apl ; 37(1)2016. ilus, tab, graf
Article in English | LILACS | ID: biblio-963612

ABSTRACT

The production and preparation of vegetables for consumption involve procedures that pose health risks to consumers. In this context the water plays an important role in the quality of minimally processed vegetables. We assessed the hygienic-sanitary quality of leafy vegetables and obtained data of interest to health surveillance agencies and public health. They were tested for the presence of total and faecal coliforms, and also parasitic protozoa and helminths. The vegetable samples were purchased in supermarkets of the Midwest of the State of Santa Catarina, Brazil. We performed bacteriological analyses of wash water samples using chromogenic substrates to obtain the most probable number of the coliform group at 35 °C and Escherichia coli. We used Hoffman's spontaneous sedimentation technique for the parasitological analysis in order to check the occurrence of heavy and light helminths eggs and protozoan cysts. The analyses indicated the presence of coliforms, nematodes and freeliving protozoa, except for the last wash. We concluded that the washing process using running water and no active chlorine was not efficient in reducing the load of microorganisms in the vegetables. Therefore, intensive educational programmes should be implemented by health authorities to encourage hygienic-sanitary practices and risk reduction of food-borne diseases.(AU)


Subject(s)
Vegetables/parasitology , Water/physiology , Coliforms/analysis , Sodium Hypochlorite/therapeutic use , Vegetables/microbiology
10.
Acta odontol. latinoam ; 29(3): 225-229, 2016. ilus, tab
Article in English | LILACS | ID: biblio-868695

ABSTRACT

The aim of this study was to analyze fungal contamination on dental chairs at the clinic of a Higher Education Institution in TeresinaPI, Brazil, and to evaluate the effectiveness of different disinfectants: 70% alcohol and 1% sodium hypochlorite. We selected the five sites with most contact between patient and chair: headrest, backrest, armrests, seat and foot rest. Samples were collected from these sites on 14 chairs and inoculated in agar Sabouraud culture medium containing chloramphenicol. Pathogenic fungi were isolated from all sampling sites on the chairs. Highest frequencies were found on footrest, followed in decreasing order by seat, backrest, armrests and headrest. Fourteen species of filamentous fungi were identified, belonging to the genera Alternaria, Aspergillus, Cladosporium, Curvularia, Drechslera, Fusarium, Penicillium and Paecillomyces. After sampling, seven chairs were disinfected with 70% alcohol and seven with 1% sodium hypochlorite, and samples were taken again using the same procedure. No fungal growth was detected following disinfection with sodium hypochlorite, which was clearly more effective than alcohol, after which there was still fungal growth. This study highlights the need for the biosafety protocol to include cleaning and disinfection of dental chairs with 1% sodium hypochlorite after each attendance in order to prevent crossinfection.


O objetivo deste estudo foi analisar contaminação fúngica em cadeiras odontológicas na clínica de uma Instituição de Educação Superior em TeresinaPI, Brasil, e avaliar a efetividade de diferentes desinfetantes: álcool 70% e hipoclorito de sódio 1%. Nós selecionamos os cinco locais com maior contato entre o paciente e a cadeira: encosto da cabeça, das costas, dos braços, assento e encosto dos pés. As amostras foram coletadas destes locais das 14 cadeiras e inoculadas em meio de cultura agarSabouraud contendo cloranfenicol. Fungos patogênicos foram isolados de todos os locais de amostragem das cadeiras. As frequências mais altas foram encontradas no encosto dos pés, seguido em ordem decrescente pelo assento, encosto das costas, dos braços e encosto da cabeça. Quatorze espécies de fungos filamentoso foram identificados, pertencente aos gêneros Alternaria, Aspergillus, Cladosporium, Curvularia, Drechslera, Fusarium, Penicillium e Paecillomyces. Após a coleta, sete cadeiras foram desinfe tadas com álcool 70% e sete com hipoclorito de sódio 1%, e as amostras foram colhidas novamente usando o mesmo procedimento. Não foi detectado crescimento fúngico após desinfecção com hipoclorito de sódio, que foi claramente mais efetivo que o álcool, do qual ainda houve crescimento fúngico.Este estudo destaca a necessidade da inclusão no protocolo de biossegurança a limpeza e desinfecção das cadeiras odontológicas com o hipoclorito 1% após cada atendimento, a fim de prevenir infecções cruzadas.


Subject(s)
Humans , Equipment Contamination/prevention & control , Infection Control, Dental/methods , Disinfection/methods , Mycoses/prevention & control , Brazil , Culture Media , Epidemiology, Descriptive , Ethanol/therapeutic use , Sodium Hypochlorite/therapeutic use , Evaluation Studies as Topic , Colony Count, Microbial/methods , Data Interpretation, Statistical
11.
Int. j. odontostomatol. (Print) ; 9(3): 399-404, dic. 2015. ilus
Article in English | LILACS | ID: lil-775463

ABSTRACT

This study determined if p-chloroaniline (PCA) can be minimized by using distilled water and physiological saline solution following sodium hypochlorite but before chlorhexidine. Hypochlorite 5%, 0.5%, 0.05%, 0.005% and 0.0005% dissolved in 0.9% NaCl and in distilled water were mixed with 2% chlorhexidine for the formation of PCA. The PCA was determined using UV-VISIBLE spectrometry, with spectral curve was determined the wavelength of maximum absorption of PCA. Formed PCA absorbance was measured between 0.025%, 0.02%, 0.015%, 0.01%, 0.005% and 0.0025% hypochlorite and 2% chlorhexidine. 2% chlorhexidine and hypochlorite with physiological saline form a white precipitate which prevents the measurement of PCA. Colored PCA is formed with 0.05%, 0.005% hypochlorite aqueous dilutions and 2% chlorhexidine. The lwavelength of maximum absorption obtained was 470 nm and absorbance of PCA showed a linear decrease. 0.005% NaClO produces the least amount of PCA. The best solvent to prevent the formation of PCA during the interaction of sodium hypochlorite with chlorhexidine is distilled water.


Este estudio determinó si la p-cloroanilina (PCA) puede ser minimizada mediante el uso de agua destilada y solución salina fisiológica seguido de la aplicación de hipoclorito de sodio, previo a la aplicación de clorhexidina. Hipoclorito al 5%, 0,5%, 0,05%, 0,005% y 0,0005% fue disuelto en 0,9% de NaCl y en agua destilada se mezcló con 2% de clorhexidina para la formación de PCA. El PCA se determinó mediante espectrometría UV-Visible, y con curva espectral se determinó la longitud de onda máxima del PCA. La absorbancia del PCA formado se midió con 0,025%, 0,02%, 0,015%, 0,01%, 0,005% y 0,0025% de hipoclorito y 2% de clorhexidina. La combinación de 2% de clorhexidina e hipoclorito en solución salina fisiológica forman un precipitado blanco que impide la medición del PCA. El PCA coloreado es formado con 0,05%, 0,005% diluciones acuosas de hipoclorito y 2% de clorhexidina. La longitud de onda máxima obtenida fue de 470 nm y la absorbancia del PCA mostró una disminución lineal. NaClO al 0,005% produce menor cantidad de PCA. El mejor disolvente para evitar la formación de PCA durante la interacción de hipoclorito de sodio con clorhexidina es agua destilada.


Subject(s)
Acrylates/toxicity , Aniline Compounds/toxicity , Sodium Hypochlorite/therapeutic use , Distilled Water , Saline Solution/therapeutic use
12.
Arq. odontol ; 51(1): 32-38, 2015. ilus, graf
Article in Portuguese | LILACS, BBO | ID: biblio-850194

ABSTRACT

Objetivo: O objetivo deste estudo foi avaliar e comparar a efetividade das soluções de hipoclorito de sódio (NaOCl) 1% e 5%, clorexidina (CHX) 2%, suspensões de nanopartículas de prata (Np Ag) 1% e nanopartículas de óxido de zinco (Np ZnO) 26% contra o biofilme de E. faecalis. Material e Métodos: Setenta e seis dentes humanos unirradiculados foram modelados, montados em um aparato específico e esterilizados. Após, 100 μL de uma suspensão de E. faecalis foi inserida nos canais, sendo renovada diariamente por 7 dias. Quatro segmentos radiculares foram analisados por meio de microscopia eletrônica de varredura (MEV) para confirmar a presença do biofilme. Os segmentos radiculares remanescentes foram divididos aleatoriamente em 6 grupos (n = 12), de acordo com a solução irrigadora empregada: G1) solução salina 0,85% (controle); G2) NaOCl 1%; G3) NaOCl 5%; G4) CHX 2%; G5) suspensão de Np Ag 1%; e G6) suspensão de Np ZnO 26%. Concluída a irrigação, a susceptibilidade do biofilme às soluções irrigadoras (n = 10) foi determinada pelo método de plaqueamento e contagem de unidades formadoras de colônias (UFC). Uma análise por meio de MEV foi conduzida em 2 segmentos de cada grupo para visualização da estrutura do biofilme. O conjunto de dados, representados pelos valores médios de UFC para cada grupo, foi analisado estatisticamente pelos testes Kruskal-Wallis e Mann-Whitney (p < 0,05). Resultados: A efetividade das soluções de NaOCl 5% e Np Ag 1% contra o biofilme intracanal de E. faecalis foi superior comparada à solução salina 0,85% (p < 0,05). NaOCl 5% reduziu 100% das UFC comparado ao grupo controle, seguido pela suspensão de Np Ag 1% (97,6%), Np ZnO 26% (96,1%), NaOCl 1% (94,1%) e CHX 2% (93,1%). Conclusão: Com base na metodologia aplicada, as soluções de NaOCl 5% e Np Ag 1% apresentaram excelente efetividade contra o biofilme de E. faecalis estabelecido no canal radicular...


Subject(s)
Disinfection , Enterococcus faecalis , Dental Plaque , Chlorhexidine/therapeutic use , Colony Count, Microbial , Sodium Hypochlorite/therapeutic use , Metal Nanoparticles
13.
Braz. oral res. (Online) ; 29(1): 1-9, 2015. tab, ilus
Article in English | LILACS | ID: lil-777212

ABSTRACT

This study aimed to evaluate, by means of scanning electron microscopy (SEM), the cleaning of flattened root canals, varying irrigation/aspiration protocols during biomechanical preparation. Thirdy human mandibular incisors were distributed into three groups (n = 10) according to the aspiration/irrigation protocols: conventional, conventional + brush, and apical negative pressure irrigation. Irrigation procedure was performed with 5 mL of 1% NaOCl at each change of instrument; final irrigation was conducted with 17% EDTA for 5 min. After biomechanical preparation, the roots were sectioned and prepared for SEM analysis. The images obtained were evaluated under 35× and 1,000× magnification by three calibrated examiners, following a double-blind design. All data were analyzed using Kruskal–Wallis and Dunn’s post hoctests (α = 0.05). Canals wherein the conventional method and apical negative pressure irrigation were employed revealed less debris, statistically different from the conventional + brush group (p < 0.05). Regarding the presence of smears, apical negative pressure irrigation was more effective in cleaning, showing lowest scores (p < 0.05), compared with the other tested protocols. Comparing each root canal third revealed that the apical portion was difficult to clean as all the tested protocols showed similar high scores (p > 0.05), both for the presence of debris and smear layer. In conclusion, although none of the studied irrigation/aspiration protocols have completely cleaned flattened root canals, apical negative pressure irrigation was more effective in smear layer removal, whereas the conventional + brush protocol was the least effective in removing the debris and smear layer.


Subject(s)
Humans , Dental Pulp Cavity/drug effects , Root Canal Irrigants/therapeutic use , Root Canal Preparation/instrumentation , Root Canal Preparation/methods , Debridement/instrumentation , Debridement/methods , Dental Pulp Cavity/anatomy & histology , Dentin/drug effects , Disinfectants/therapeutic use , Edetic Acid/therapeutic use , Microscopy, Electron, Scanning , Reproducibility of Results , Statistics, Nonparametric , Surface Properties , Smear Layer/therapy , Sodium Hypochlorite/therapeutic use , Time Factors , Treatment Outcome
14.
Rev. Assoc. Paul. Cir. Dent ; 68(4): 357-363, out.-dez. 2014. ilus, tab
Article in Portuguese | LILACS, BBO | ID: lil-745437

ABSTRACT

O objetivo deste estudo clínico prospectivo randomizado foi analisar a influência de duas substâncias quimicas auxiliares na identificação de canais acessórios e no selamento do forame apical pelo cimento endodôntico e a prevalência desses canais na faixa etária de 13 a 79 anos, nos diferentes grupos dentários, em dentes vitais, não vitais e nos retratamentos endodônticos. Trezentos e um tratamentos endodônticos foram concluídos em 240 pacientes. f';s substâncias químicas auxiliares utilizadas foram a clorexidina gel 2% (n= 145) e o hipoclorito de sódio 5.25% (n= 156). Os dentes foram preparados pela técnica coroa-ápice com patência e ampliação do fora me apical e obturados pela técnica de termoplastificação da guta-percha. Três observadores independentes (triplo cego) avaliaram, radiograficamente, o preenchimento de canais acessórios e do forame apical que foi registrado e expresso em dados percentuais. Os dados foram analisados, estatisticamente, e os resultados mostraram que o forame apical foi selado pelo cimento endodôntico em 98.34% (296/301) e os canais acessórios foram preenchidos e visíveis radiograficamente em 47.84% (144/301) dos dentes tratados. Canais acessórios foram encontrados em 55% (99/180) dos dentes multirradiculares e em 37.20% (45/121) dos dentes unirradiculares. De acordo com o estado pulpar, canais acessórios foram encontrados em 55,20% (69/125) dos dentes vitais, 49.00% (49/100) dos dentes não vitais e 34.21% (26/76) dos retratamentos endodônticos. Concluiu-se que, entre os fatores analisados, não houve diferença estatisticamente significativa entre os dois grupos das substâncias químicas auxiliares analisadas.


The aim of this prospective randomized study, conducted in vivo, was to analyze the influence of two auxiliary chemicals in filling accessory canais and apical foramen by sealer and the prevalence of these channels in the age group 13 to 79 years, in various dental groups, in vital and non-vital teeth and root canal retreatment. Two hundred forty patients were treated and 301 endodontic treatments were completed. The auxiliary chemicals used were 2% chlorhexidine gel [n = 145) and 5.25% sodium hypochlorite (n = 156). The teeth were instrumented by crown-down technique with patency and enlargement of the apical foramen and obturated by the thermoplastic gutta-percha technique. Three independent observers (triple blind) evaluated radiographically obturation of accessory canais and apical foramen by the sealer that was recorded and expressed as a percentage. Data were statistically analyzed using the Binominal and Qui Quadrado tests. The results showed that the apical foramen was filled in 98.34% (296/301) and the accessories channels were filled and radiographically visible in 47.84% (144/301) of teeth treated. Accessory canais were found in 55% (99/180) of the teeth multirooted and 37.20% (45/121) of single-rooted teeth. According to the state of pulp, accessory canais were found in 55.20% (69/125) of vital teeth, 49.000/0 (49/100) of non-vital teeth and 34.21% (26/76) of endodontic retreatment. It was concluded that among the factors examined, there was no statistically significant difference between the two groups.


Subject(s)
Humans , Male , Female , Chlorhexidine/therapeutic use , Extravasation of Diagnostic and Therapeutic Materials/diagnosis , Sodium Hypochlorite/therapeutic use , Root Canal Therapy/methods
15.
J. appl. oral sci ; 22(6): 490-495, Nov-Dec/2014. tab
Article in English | LILACS, BBO | ID: lil-732592

ABSTRACT

Objective: The aim of this study was to monitor the effectiveness of root canal procedures by using different irrigants and intracanal medication on endotoxin levels found in root canals of teeth with chronic apical periodontitis. Material and Methods: Thirty root canals of teeth with pulpal necrosis associated with periapical lesions were selected and randomly divided into groups according to the irrigants used: GI - 2.5% NaOCl, GII - 2% chlorhexidine (CHX) gel, and GIII - saline solution (SS) (all, n=10). Samples were collected with sterile/apyrogenic paper points before (S1) and after root canal instrumentation (S2), after use of 17% ethylenediaminetetraacetic acid (EDTA) (S3), and after 30 days of intracanal medication (Ca(OH)2+SS) (S4). A turbidimetric kinetic Limulus Amebocyte Lysate assay was used for endotoxin measurement. Results: Endotoxins were detected in 100% of the root canals investigated (30/30), with a median value of 18.70 EU/mL. After S2, significant median percentage reduction was observed in all groups, irrespective of the irrigant tested: 2.5% NaOCl (99.65%) (GI), 2% CHX (94.27%) (GII), and SS (96.79%) (GIII) (all p<0.05). Root canal rinse with 17% EDTA (S3) for a 3-minute period failed to decrease endotoxin levels in GI and a slight decrease was observed in GII (59%) and GIII (61.1%) (all p>0.05). Intracanal medication for 30 days was able to significantly reduce residual endotoxins: 2.5% NaOCl (90%) (GI), 2% CHX (88.8%) (GII), and SS (85.7%) (GIII, p<0.05). No differences were found in the endotoxin reduction when comparing s2 and s4 treatment groups. Conclusion: Our findings demonstrated the effectiveness of the mechanical action of the instruments along with the flow and backflow of irrigant enduring root canal instrumentation for the endotoxin removal from root canals of teeth with chronic apical periodontitis. Moreover, the use of intracanal medication for 30 days contributed for an improvement ...


Subject(s)
Humans , Lipopolysaccharides/analysis , Periapical Periodontitis/therapy , Root Canal Irrigants/pharmacology , Root Canal Preparation/methods , Chlorhexidine/pharmacology , Chlorhexidine/therapeutic use , Chronic Disease , Dental Pulp Necrosis , Edetic Acid/pharmacology , Edetic Acid/therapeutic use , Enzyme-Linked Immunosorbent Assay , Limulus Test , Periapical Periodontitis/microbiology , Random Allocation , Root Canal Irrigants/therapeutic use , Sodium Hypochlorite/pharmacology , Sodium Hypochlorite/therapeutic use , Statistics, Nonparametric , Time Factors , Treatment Outcome
16.
Braz. j. oral sci ; 13(3): 242-245, Jul-Sep/2014. tab
Article in English | LILACS | ID: lil-725349

ABSTRACT

AIM: To evaluate antimicrobial ability of neem leaf extract, 3% sodium hypochlorite (NaOCl) and 2% chlorhexidine (CHX) against Candida albicans. METHODS: Neem leaf extract was prepared by using absolute ethanol with fresh neem leaves, filtering the extract through muslin cloth, coarse residue and filter paper. Cultures of C. albicans were maintained on Brain Heart Infusion broth and Agar. The antimicrobial efficacy was checked using the Agar Diffusion and the zones of inhibition were measured. The results were statistically analysed using ANOVA test. Inter-group comparison was checked using Kruskal Wallis ANOVA and Mann Whitney tests (a=0.05). RESULTS: There was statistically significant difference between the zones of inhibition seen with 3% NaOCl and Neem extract with 2% CHX (p<0.05). There was no significant difference observed between Neem extract and 3% NaOCl. CONCLUSIONS: Efficacy of Neem extract is comparable to 3% NaOCl against C. albicans and it is significantly better than 2% CHX...


Subject(s)
Mouthwashes/therapeutic use , Azadirachta/therapeutic use , Candida albicans , Chlorhexidine/therapeutic use , Dental Pulp Cavity , Endodontics , Sodium Hypochlorite/therapeutic use , In Vitro Techniques , Periapical Periodontitis
17.
Article in English | IMSEAR | ID: sea-154668

ABSTRACT

Aims: Comparative evaluation of cleaning efficacy of smear layer removal by different irrigating solutions such as 2.5% sodium hypochlorite (NaOCl), 17% ethylenediaminetetraacetic acid (EDTA) with 2.5% NaOCl, 10% citric acid with 2.5% NaOCl and 1% tetracycline Hydrochloride (HCl) with 2.5% NaOCl for smear layer removal in the apical third of root canal. Settings and Design: In vitro material science study. Materials and Methods: Seventy-five single rooted permanent maxillary central incisor teeth were subjected to standardized root canal instrumentation (crown down technique). The teeth were randomly divided into five groups with 15 teeth in each groups: (1) Normal saline (n = 15) (2) 2.5% NaOCl (n = 15) (3) 17% EDTA + 2.5% NaOCl (n = 15) (4) 10% citric acid + 2.5% NaOCl (n = 15) (5) 1.0% tetracycline HCL + 2.5% NaOCl (n = 15). After final irrigation, the teeth were prepared for scanning electron microscope analysis to evaluate the cleaning of apical third of radicular dentine to determine the presence or absence of smear layer. Statistical Analysis Used: The results were analyzed by nonparametric statistical analysis techniques. Kruskal-Wallis test, Mann-Whitney test and Chi-square tests were carried out. Results: There was no significant statistical difference in the efficacy of smear layer removal when 2.5% NaOCl was compared with 17% EDTA with 2.5% NaOCl, 10% citric acid with 2.5% NaOCl and 1% tetracycline HCl with 2.5% NaOCl in apical third of root canals. Conclusions: The present study suggests that irrigating agents, citric acid and tetracycline HCl can be used as an alternative to EDTA for the removal of smear layer in endodontics.


Subject(s)
Citric Acid/therapeutic use , Edetic Acid/therapeutic use , Microscopy, Electron, Scanning/statistics & numerical data , Root Canal Preparation/methods , Smear Layer , Sodium Hypochlorite/therapeutic use , Tetracycline/therapeutic use
18.
Pesqui. vet. bras ; 34(2): 109-113, fev. 2014. ilus, tab
Article in English | LILACS | ID: lil-709851

ABSTRACT

Biofilms constitute a physical barrier, protecting the encased bacteria from detergents and sanitizers. The objective of this work was to analyze the effectiveness of sodium hypochlorite (NaOCl) against strains of Staphylococcus aureus isolated from raw milk of cows with subclinical mastitis and Staphylococcus aureus isolated from the milking environment (blowers and milk conducting tubes). The results revealed that, in the presence of NaOCl (150ppm), the number of adhered cells of the twelve S. aureus strains was significantly reduced. When the same strains were evaluated in biofilm condition, different results were obtained. It was found that, after a contact period of five minutes with NaOCl (150ppm), four strains (two strains from milk , one from the blowers and one from a conductive rubber) were still able to grow. Although with the increasing contact time between the bacteria and the NaOCl (150ppm), no growth was detected for any of the strains. Concerning the efficiency of NaOCl on total biofilm biomass formation by each S. aureus strain, a decrease was observed when these strains were in contact with 150 ppm NaOCl for a total period of 10 minutes. This study highlights the importance of a correct sanitation protocol of all the milk processing units which can indeed significantly reduce the presence of microorganisms, leading to a decrease of cow´s mastitis and milk contamination.


Biofilmes são constituídos de bactérias aderidas a uma superfície e aderidas entre si envolvidas por um polissacarídeo de constituição proteica, lipídica e glicídica que conferem uma barreira física às bactérias dentro deste microambiente. O objetivo deste trabalho foi analisar a eficácia do hipoclorito de sódio (NaOCl) contra estirpes de Staphylococcus aureus isoladas de leite cru de vacas com mastite subclínica e Staphylococcus aureus isolados do ambiente de ordenha (borrachas de ordenhadeiras e mangueiras condutoras de leite). Os resultados revelaram que, na presença de hipoclorito de sódio (150ppm), o número de células aderidas das 12 estirpes de S. aureus analisadas foi significativamente reduzido. Quando as mesmas estirpes foram avaliadas em condições de biofilme, diferentes resultados foram obtidos. Verificou-se que, após um período de contato de cinco minutos com NaOCl (150ppm), quatro estirpes (duas estirpes de leite, uma estirpe das borrachas das ordenhadeiras e uma estirpe de uma mangueira condutora de leite) ainda eram capazes de crescer. Com o aumento do tempo de contato do hipoclorito e as bactérias, cada vez maior, na concentração de 150ppm, não foi detectado o crescimento das estirpes. Em relação à eficácia do NaOCl na formação total da biomassa do biofilme por cada uma das estirpes de S. aureus, observou-se decréscimo da biomassa dos biofilmes quando estas estirpes estavam em contato com o NaOCl na concentração de 150ppm durante um tempo total de 10 minutos. O estudo demonstra a importância de um protocolo de saneamento correto de todas as unidades de processamento de leite, que pode, efetivamente, reduzir a presença de microrganismos de forma significativa, conduzindo a uma diminuição da mastite e da contaminação do leite.


Subject(s)
Animals , Female , Biofilms/growth & development , Cattle , Sodium Hypochlorite/therapeutic use , Mastitis, Bovine/prevention & control , Staphylococcus aureus/growth & development , Milk/microbiology , Microscopy, Fluorescence/veterinary
19.
Braz. dent. j ; 24(6): 580-584, Nov-Dec/2013. tab, graf
Article in English | LILACS | ID: lil-697653

ABSTRACT

This study evaluated the effectiveness of a highly flexible endodontic brush made of polypropylene canal brush (CanalBrush; Coltène) on smear layer removal from the canal walls when used according to the manufacturer's recommendations. Forty-four single-rooted mandibular incisors were prepared to apical size 30/0.06 and randomly divided into three groups A, B and C, where the final irrigation regimen was 10 mL 17% EDTA and 10 mL 2.5% NaOCl for group A, 10 mL EDTA, 5 mL NaOCl, CanalBrush for 20 s at 450 rpm and 5 mL NaOCl for group B, 10 mL NaOCl, CanalBrush and 10 mL NaOCl for group C. One medium-sized CanalBrush was used for each root canal and all brushes were examined under the optical microscope after application to evaluate bristle deformation. Afterwards, roots were split longitudinally and the presence of smear layer was evaluated under a scanning electron microscope. Used brushes invariably exhibited bristle deformation. Group C exhibited the highest means of smear layer in all thirds. Comparing the apical thirds in all groups, there was no statistical difference between groups A and B (3.64±0.48 and 3.68±0.62 respectively), while group C exhibited significantly higher scores (3.9±0.28) than the other two groups. In conclusion, the CanalBrush proved unable to remove smear layer from the instrumented root canals, when used according to the manufacturers' instructions.


O objetivo deste estudo foi avaliar a eficácia de um escova intra-canal (CanalBrush, Coltène) na remoção da smear layer das paredes do canal radicular, quando usada de acordo com as instruções do fabricante. Quarenta e quatro incisivos mandibulares unirradiculares foram instrumentados até o ápice com instrumentos 30/0.06 e aleatoriamente distribuidos em três grupos A, B e C, com regime de irrigação final de 10 mL a 17% EDTA e 10 mL NaOCl a 2,5% para o Grupo A; irrigação final de 10 mL EDTA, 5 mL NaOCl, uso da escovinha CanalBrush por 20 s a 450 rpm e 5 mL NaOCl para o Grupo B e 10 mL NaOCl, uso da escovinha CanalBrush e 10 mL NaOCl para o Grupo C. Foi utilizada escovinha CanalBrush de tamanho médio para cada canal e todas elas foram examinadas em microscópio para avaliação da deformação de cerdas. Depois as raízes foram cortadas longitudinalmente e a presença da smear layer foi avaliada por microscópio eletrônico de varredura. As escovinhas usadas apresentaram todas alguma deformação das cerdas. No Grupo C foi observada a maior média de área de smear layer em todos os terços dentais. Na comparação dos terços apicais de todos os grupos não houve diferença significativa entre os grupos A e B (3,64±0.48 e 3,68±0,62, respectivamente), enquanto que o Grupo C mostrou escores significativamente mais altos(3,9±0.28) que os outros dois grupos. Concluindo, a escovinha CanalBrush mostrou ser incapaz de remover a smear layer dos canais instrumentados, quando usada de acordo com as instruções do fabricante.


Subject(s)
Humans , Root Canal Irrigants/therapeutic use , Root Canal Preparation/instrumentation , Smear Layer , Edetic Acid/therapeutic use , In Vitro Techniques , Incisor , Microscopy, Electron, Scanning , Polypropylenes , Sodium Hypochlorite/therapeutic use
20.
Braz. dent. j ; 24(3): 188-193, May-Jun/2013. tab, graf
Article in English | LILACS | ID: lil-681867

ABSTRACT

This study investigated the expression of extracellular matrix glycoproteins tenascin (TN) and fibronectin (FN) in pulp repair after capping with calcium hydroxide (CH), following different hemostasis protocols. Class I cavities with a pulp exposure were prepared in 42 human third molars scheduled for extraction. Different hemostatic agents (0.9% saline solution, 5.25% sodium hypochlorite and 2% chlorhexidine digluconate) were used and pulps were capped with CH cement. After 7, 30 or 90 days, teeth were extracted, formalin-fixed, and prepared for immunohistochemical technique. Hemostatic agents did not influence the expression of TN and FN. Both glycoproteins were found in the entire the pulp tissue and around collagen fibers, but were absent in the mineralized tissues. In the predentin, TN showed positive immunostaining and FN had a variable expression. Within 7 days post-treatment, a slightly more pronounced immunostaining on the pulp exposure site was observed. Within 30 days, TN and FN demonstrated a positive expression around the dentin barrier and at 90 days, a thin and linear expression of TN and FN was delimitating the reparative dentin. In conclusion, hemostatic agents did not influence TN and FN expression. Immunostaining for TN and FN was seen in different regions and periods, demonstrating their role in pulp repair.


Este estudo investigou a expressão das glicoproteínas Tenascina (TN) e Fibronectina (FN) da matriz extracelular no reparo pulpar após capeamento com hidróxido de cálcio (HC), seguindo diferentes protocolos de hemostasia. Cavidades de classe I com exposição pulpar foram preparadas em 42 terceiros molares humanos indicados para extração. Diferentes agentes hemostáticos (solução salina a 0,9%, hipoclorito de sódio a 5,25% e clorexidina a 2%) foram usados e as polpas foram capeadas com cimento de HC. Após 7, 30 ou 90 dias, os dentes foram extraídos, fixados em formalina e preparados para análise imunoistoquímica. Os agentes hemostáticos não influenciaram a expressão de TN e FN. Ambas glicoproteínas foram encontradas em todo tecido pulpar, ao redor das fibras colágenas e estiveram ausentes nos tecidos mineralizados. Na pré-dentina, a TN mostrou forte imunoexpressão e a FN teve uma expressão variável. Após 7 dias, foi observada uma expressão levemente mais pronunciada no lugar da exposição pulpar. Aos 30 dias, a TN e a FN demonstraram uma expressão mais forte sob a barreira dentinária e aos 90 dias, uma expressão fina e linear da TN e FN apresentava-se delimitando a dentina reparativa. Em conclusão, os agentes hemostáticos não influenciaram e expressão da TN e da FN. A imunoexpressão da TN e FN foi observada em diferentes regiões e períodos, demonstrando o seu papel no reparo pulpar.


Subject(s)
Adult , Humans , Young Adult , Dental Pulp Capping , Fibronectins/analysis , Hemostatics/therapeutic use , Pulp Capping and Pulpectomy Agents/therapeutic use , Tenascin/analysis , Bisphenol A-Glycidyl Methacrylate/chemistry , Calcium Hydroxide/therapeutic use , Chlorhexidine/analogs & derivatives , Chlorhexidine/therapeutic use , Collagen/analysis , Composite Resins/chemistry , Dental Pulp Exposure/therapy , Dental Pulp/chemistry , Dental Restoration, Permanent/methods , Dentin, Secondary/chemistry , Dentin/chemistry , Follow-Up Studies , Sodium Chloride/therapeutic use , Sodium Hypochlorite/therapeutic use , Tooth Extraction
SELECTION OF CITATIONS
SEARCH DETAIL