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1.
Acta bioquím. clín. latinoam ; 54(4): 395-406, jul. 2020. tab, graf
Article in Spanish | LILACS | ID: biblio-1149029

ABSTRACT

Resumen El objetivo del presente trabajo fue el desarrollo de dos enzimoinmunoensayos competitivos (EIC) para la detección de trazas de soja y de leche en productos libres de gluten. Como anticuerpos primarios se utilizaron antisueros policlonales de conejo específicos contra proteínas de soja o de leche. Se determinaron las concentraciones óptimas de antígenos a inmovilizar en la placa y las concentraciones de anticuerpos primarios a utilizar en la competencia. Las curvas de calibración se ajustaron utilizando concentraciones crecientes de un extracto de producto de soja y de un extracto de leche descremada en polvo. El producto de soja y la leche descremada se extrajeron con buffer Tris-HCl 0,0625 M con dodecilsulfato de sodio al 3% y sulfito de sodio 0,1 M al 2%. Se evaluaron los parámetros de validación: linealidad, límites de detección y de cuantificación, recuperación y precisión en el día y entre días, los cuales resultaron adecuados. Se analizaron 9 productos libres de gluten con los EIC desarrollados y con kits de ELISA comerciales. Ambos EIC se comportaron de manera similar con respecto a los kits comerciales. Los EIC permitieron confirmar la presencia de leche en las muestras que la declaraban. En algunas muestras que no declaraban ni leche ni soja, ambos EIC detectaron su presencia (resultados confirmados con los kits comerciales). Los EIC desarrollados poseen menor costo que los kits y, por lo tanto, éstos podrían utilizarse como métodos de screening. Cuando esta metodología resulte negativa, debe confirmarse con un método más sensible (comercial) para garantizar la ausencia de proteínas de soja o de leche.


Abstract The aim of this study was to develop two competitive enzyme immunoassays (CEI) to detect the presence of traces of soy and milk in gluten-free products. Specific rabbit polyclonal antiserums against soy protein and other against elemilk protein were used as primary antibodies. Optimal antigen concentrations to be immobilized on the plate and primary antibody concentrations to be used in competition were determined. The calibration curves were fitted using increasing concentrations of an extract of soy product and of defatted milk powder. The soy product and the defatted milk were extracted with Tris-HCl buffer 0,0625 M with 3% sodium dodecyl sulfate and 2% sodium sulfite 0.1 M. The validation parameters were evaluated: linearity, limit of detection and quantification, recovery and precision on the day and in between days. They were appropriate. Nine commercial samples of gluten-free products were analyzed with these developed CEI and commercial ELISA kits. It was observed that both CEI behaved similarly with respect to the commercial kits. The enzyme immunoassays confirmed the presence of milk in samples that declared it. In some samples that did not declare the presence of milk or soy, both enzyme immunoassays detected their presence -these results were confirmed using commercial kits. The developed CEI have a lower cost than the commercial kits, so these could be used as screening methods. When this methodology is negative, it should be confirmed with a more sensitive (commercial) method to ensure the absence of soy or milk protein.


Resumo O objetivo do presente trabalho foi o desenvolvimento de dois enzimoimunoensaios competitivos (EIC), para a detecção de vestígios de soja e leite em produtos livres de glúten. Antissoros policlonais de coelho específicos contra proteínas de soja ou de leite foram utilizados como anticorpos primários. Foram determinadas as concentrações ótimas de antígenos a serem imobilizados na placa e as concentrações de anticorpos primários a serem utilizadas na competição. As curvas de calibração foram ajustadas usando concentrações crescentes de um extrato de produto de soja e de um extrato de leite em pó desnatado. O produto de soja e o leite desnatado foram extraídos com tampão Tris-HCl 0,0625 M com dodecil sulfato de sódio a 3% e sulfito de sódio 0,1 M a 2%. Os parâmetros de validação foram avaliados: linearidade, limite de detecção e quantificação, recuperação e precisão no dia e entre os dias, os quais resultaram adequados. Nove produtos livres de glúten foram analisados com os EIC desenvolvidos e com kits de ELISA comerciais. Os dois EICs se comportaram de maneira semelhante em relação aos kits comerciais. Os EIC permitiram confirmar a presença de leite nas amostras que o declararam. Em algumas amostras que declaravam nem leite nem soja, ambos os EIC detectaram sua presença (resultados confirmados usando kits comerciais). Os EIC desenvolvidos têm um custo menor que os kits, portanto, eles poderiam ser utilizados como métodos de triagem. Quando esta metodologia é negativa, deve ser confirmada com um método mais sensível (comercial) para garantir a ausência de proteínasda soja ou do leite.


Subject(s)
Enzyme-Linked Immunosorbent Assay/methods , Soybean Proteins/analysis , Diet, Gluten-Free , Food Analysis/methods , Milk Proteins/analysis , Sodium Dodecyl Sulfate , Enzyme-Linked Immunosorbent Assay/economics , Costs and Cost Analysis , Sodium Sulfite , Food Technology/methods
2.
Rev. chil. nutr ; 47(3): 463-469, jun. 2020. tab
Article in English | LILACS | ID: biblio-1126145

ABSTRACT

The addition of soy proteins, currently classified as a food allergen, into meat products is a commonly used practice due to its functional properties and low cost. Its addition to meat products can cause health problems for individuals allergic to these proteins. Allergic individuals can be affected by the ingestion of low amounts of the allergen. In Brazil, limits are set for the addition of soy proteins in meat products in order to avoide fraud. Starting in 2015 reporting the name of the added component became mandatory for all food labelling. Some studies have reported that food processing can reduce the allergenicity, either by irreversible removal of allergens or by modifying the allergen structure. However, the technological approach to decrease allergenicity has largely been empirical. This review describes the use of soy protein in meat products and the health risk for allergic individuals and consumers of these products. Finally, appropriate methodologies for the detection and quantification of these proteins must be further explored and established to avoid fraud and to preserve consumer health.


La adición de proteínas de soya, actualmente clasificadas como alergeno alimentario, en los productos cárnicos es una práctica comúnmente utilizada debido a sus propiedades funcionales y bajo costo. Su adición en productos cárnicos puede causar problemas de salud en personas alérgicas a estas proteínas. Las personas alérgicas pueden verse afectadas por la ingestión de cantidades diminutas de alérgeno. En Brasil, se establecen límites para la adición de proteínas de soya en los productos cárnicos con el objetivo de evitar el fraude. Solo en 2015 se hizo obligatoria la declaración en la etiqueta de todos los alimentos que indicaban la presencia de sustancias alérgicas, así como el nombre del componente. Algunos estudios se refieren al procesamiento de alimentos para reducir la alergenicidad, ya sea mediante la eliminación irreversible de alergenos o modificando la estructura del alergeno; sin embargo, el enfoque tecnológico hasta ahora para disminuir la alergenicidad ha sido en gran medida empírico. Esta revisión describe el uso de proteína de soya en los productos cárnicos y el riesgo que puede causar para la salud de las personas alérgicas y a los consumidores de estos productos. Finalmente, las metodologías apropiadas para la detección y cuantificación de estas proteínas deben explorarse en profundidad y establecerse para evitar el fraude y preservar la salud de los consumidores.


Subject(s)
Humans , Plant Proteins, Dietary/adverse effects , Soybean Proteins/adverse effects , Food Hypersensitivity/etiology , Meat Products , Allergens , Health Risk , Food Hypersensitivity/prevention & control , Epitopes
3.
Medisan ; 21(1)ene. 2017.
Article in Spanish | LILACS, CUMED | ID: biblio-841649

ABSTRACT

La lecitina de soya, producto natural empleado como suplemento nutricional, presenta múltiples acciones biológicas demostradas, por lo cual resulta muy beneficiosa para tratar a pacientes con distintas afecciones. Teniendo en cuenta lo anterior se realizó la presente investigación donde se exponen algunos aspectos de interés, con vistas a difundir aún más lo relacionado con esta temática


The soy phosphatidylcholine, natural product used as nutritional supplement, presents multiple demonstrated biological actions, reason why it is very beneficial to treat patients with different disorders. Taking into account the above-mentioned the present investigation was carried out where some aspects of interest are exposed, aimed at diffusing even more everything related to this thematic


Subject(s)
Humans , Male , Female , Soybeans , Soy Foods , Lecithins/therapeutic use , Lecithins/pharmacology , Phytohemagglutinins , Soybean Proteins/therapeutic use , Dietary Supplements , Nutritive Value
4.
Braz. j. microbiol ; 47(4): 941-948, Oct.-Dec. 2016. tab, graf
Article in English | LILACS | ID: biblio-828200

ABSTRACT

Abstract In this study, physiological aspects of Lactobacillus plantarum BL011 growing in a new, all-animal free medium in bioreactors were evaluated aiming at the production of this important lactic acid bacterium. Cultivations were performed in submerged batch bioreactors using the Plackett-Burman methodology to evaluate the influence of temperature, aeration rate and stirring speed as well as the concentrations of liquid acid protein residue of soybean, soy peptone, corn steep liquor, and raw yeast extract. The results showed that all variables, except for corn steep liquor, significantly influenced biomass production. The best condition was applied to bioreactor cultures, which produced a maximal biomass of 17.87 g L-1, whereas lactic acid, the most important lactic acid bacteria metabolite, peaked at 37.59 g L-1, corresponding to a productivity of 1.46 g L-1 h-1. This is the first report on the use of liquid acid protein residue of soybean medium for L. plantarum growth. These results support the industrial use of this system as an alternative to produce probiotics without animal-derived ingredients to obtain high biomass concentrations in batch bioreactors.


Subject(s)
Soybean Proteins , Culture Media , Lactobacillus plantarum/growth & development , Lactobacillus plantarum/metabolism , Biomass , Lactic Acid/biosynthesis , Soybean Proteins/metabolism , Soybean Proteins/chemistry , Bioreactors , Culture Media/chemistry , Carbohydrate Metabolism , Fermentation , Hydrolysis
5.
Chinese Journal of Biotechnology ; (12): 657-668, 2016.
Article in Chinese | WPRIM | ID: wpr-337433

ABSTRACT

Metabolism and deposition of exogenous gene and protein from transgenic glyphosate herbicide-tolerant soybean meal in SD rats were studied in the experiment. The transgenic soybean GTS40-3-2 meal and its non-transgenic counterpart (parent A5403) were fed to the generation and the second generation Sprague-Dawley (SD) rats (Rattus norvegicus). The study added the genetically modified (GM) soybean meal and its non-transgenic control soybean meal (parent A5403) in a ratio of 20% respectively to the feeds. By using qualitative, quantitative PCR and ELISA methods to detect transgenic soybean residues of metabolism ingredients in rats, the safety and influence of GM soybean were evaluated. The results showed that the intestinal fecal and cecum contents of rats were detected with residues of GM ingredients, intestinal flora and organs were not found related genes and protein. These results indicated that transgenic glyphosate herbicide-tolerant soybean GTS40-3-2 meal was as safe as its non-GM soybean meal in long-term feeding study.


Subject(s)
Animal Feed , Animal Nutritional Physiological Phenomena , Animals , Digestion , Glycine , Herbicide Resistance , Herbicides , Plants, Genetically Modified , Proteolysis , Rats , Rats, Sprague-Dawley , Soybean Proteins , Soybeans
6.
Yonsei Medical Journal ; : 681-689, 2016.
Article in English | WPRIM | ID: wpr-21845

ABSTRACT

PURPOSE: Clinical trials have studied the use of soy protein for treating type 2 diabetes (T2D) and metabolic syndrome (MS). The purpose of this study was to outline evidence on the effects of soy protein supplementation on clinical indices in T2D and MS subjects by performing a meta-analysis of randomized controlled trials (RCTs). MATERIALS AND METHODS: We searched PubMed, EMBASE, and Cochrane databases up to March 2015 for RCTs. Pooled estimates and 95% confidence intervals (CIs) were calculated by the fixed-and-random-effects model. A total of eleven studies with eleven clinical variables met the inclusion criteria. RESULTS: The meta-analysis showed that fasting plasma glucose (FPG) [weighted mean difference (WMD), -0.207; 95% CI, -0.374 to -0.040; p=0.015], fasting serum insulin (FSI) (WMD, -0.292; 95% CI, -0.496 to -0.088; p=0.005), homeostasis model of assessment for insulin resistance index (HOMA-IR) (WMD, -0.346; 95% CI, -0.570 to -0.123; p=0.002), diastolic blood pressure (DBP) (WMD, -0.230; 95% CI, -0.441 to -0.019; p=0.033), low-density lipoprotein cholesterol (LDL-C) (WMD, -0.304; 95% CI, -0.461 to -0.148; p=0.000), total cholesterol (TC) (WMD, -0.386; 95% CI, -0.548 to -0.225; p=0.000), and C-reactive protein (CRP) (WMD, -0.510; 95% CI, -0.722 to -0.299; p=0.000) are significant reduced with soy protein supplementation, compared with a placebo control group, in T2D and MS patients. Furthermore, soy protein supplementation for longer duration (≥6 mo) significantly reduced FPG, LDL-C, and CRP, while that for a shorter duration (<6 mo) significantly reduced FSI and HOMA-IR. CONCLUSION: Soy protein supplementation could be beneficial for FPG, FSI, HOMA-IR, DBP, LDL-C, TC, and CRP control in plasma.


Subject(s)
Aged , Blood Glucose/metabolism , Blood Pressure , C-Reactive Protein/metabolism , Cardiovascular Diseases/prevention & control , Cholesterol/blood , Diabetes Mellitus, Type 2/blood , Dietary Supplements , Humans , Lipids/blood , Metabolic Syndrome/blood , Randomized Controlled Trials as Topic , Soybean Proteins/administration & dosage , Soybeans
7.
Article in English | WPRIM | ID: wpr-264616

ABSTRACT

By now, the digestive stability experiments provided by most authoritative organizations are in vitro tests. Evaluating the protein digestive stability with in vivo models should be more objective. The present study aimed to verify the in vivo digestibility of soybean β-conglycinin β-subunit in Wuzhishan (WZS) minipigs. Three minipigs were surgically fitted with O-stomach and T-ileum cannulae and fed with soybean meals. According to SDS-PAGE, the 50 kD fraction of soybean β-conglycinin β-subunit persisted in the gastric fluid until 6 h after feeding, which was detected at 3 h and clearly visible at 4-6 h in the intestinal fluid. Western blot with anti-β-conglycinin β-subunit McAb confirmed it.


Subject(s)
Animals , Antigens, Plant , Chemistry , Metabolism , Digestion , Physiology , Globulins , Chemistry , Metabolism , Male , Protein Subunits , Chemistry , Metabolism , Seed Storage Proteins , Chemistry , Metabolism , Soybean Proteins , Chemistry , Metabolism , Swine , Swine, Miniature , Physiology
10.
Article in Chinese | WPRIM | ID: wpr-236363

ABSTRACT

<p><b>OBJECTIVE</b>To observe the effect of the postpone in skeletal muscle aging process of D-galactose rats by weight training and soy polypeptide supplement in 6 weeks, and discuss the initial mechanism.</p><p><b>METHOD</b>Sixty male SD rats (three month old)were randomly assigned: 6 week control (C6,) and 6 week model (M6) 6 for each group, 12 week model (M12), big load (B12), small load (S12), peptide (P12), peptide + big load (PB12) and peptide + small load group (PS12) 8 for each group, eight fourteen month rats were taken in the natural aging group. The rats were killed by the end of 6th week and 12th week, tested the indicators.</p><p><b>RESULT</b>Compare with group C6, the indicators in group M6 showed aging in different levels; Compare with group M12, weight training or soy polypeptide supplement in all intervention groups could increase the content of skeletal muscle superoxide dismutase (SOD), SOD/MDA, the serum growth hormone(GH), insulin-like growth factor-1 (IGF-I)and skeletal muscle IGF-I mRNA, decreased the malondialdehyde (MDA) content of skeletal muscle, and they had notable interaction.</p><p><b>CONCLUSION</b>Rat skeletal muscle aging model can be copied successfully by D-galactose hypodermic, and go on with 6-week weight training or soy polypeptide supplement, they can postpone the skeletal muscle aging process of D-galactose rats, and the two interference way united can have more obvious effect. Its preliminary mechanism may be related to the reduction of skeletal muscle oxidative stress and lipid peroxidation, the correction of hormones and related factors metabolic disorders, the elevation of skeletal muscle IGF-I mRNA expression and so on.</p>


Subject(s)
Aging , Physiology , Animals , Galactose , Growth Hormone , Blood , Insulin-Like Growth Factor I , Metabolism , Male , Malondialdehyde , Metabolism , Muscle, Skeletal , Physiology , Physical Conditioning, Animal , Physiology , Rats , Rats, Sprague-Dawley , Soybean Proteins , Pharmacology , Soybeans , Chemistry , Superoxide Dismutase , Metabolism
11.
Article in Chinese | WPRIM | ID: wpr-236311

ABSTRACT

<p><b>OBJECTIVE</b>To explore the effect of Se-riched soybean peptide (SSP) on antioxidant function in rats of fatty liver caused by high-fat diet.</p><p><b>METHODS</b>Forty Wistar rats were divided into 4 groups randomly and fed with standard diet and water (NC), high-fat diet and water (HC), high-fat diet and SSP (0.1 g/d) (SeH), standard diet and SSP (0.1 g/d) (SeN) respectively. After 10 weeks, the rats were killed to investigate the pimelosis level in liver tissues by Sudan III staining and the expression of hepatic GRP78 by immunohistochemical analysis. We also analyzed the changes of liver function, blood lipid, the glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in livers and serum.</p><p><b>RESULTS</b>The pimelosis level, total cholesterol (TC), triglyceride (TG), MDA contents and the expression of GRP78 in HC group were significantly higher than those in NC, SeN, SeH groups. The activities of GSH-Px and SOD in liver and serum were markedly up-regulated in SeH (P < 0.01). There was no significant difference between NC and SeN groups.</p><p><b>CONCLUSION</b>SSP can improve liver cell injury and the antioxidant functions in rats with fatty liver effectively and decrease the expression of GRP78 in liver.</p>


Subject(s)
Animals , Antioxidants , Metabolism , Diet, High-Fat , Disease Models, Animal , Fatty Liver , Metabolism , Heat-Shock Proteins , Metabolism , Liver , Metabolism , Male , Rats , Rats, Wistar , Selenium , Pharmacology , Soybean Proteins , Pharmacology , Soybeans , Chemistry
12.
Chinese Journal of Biotechnology ; (12): 1709-1719, 2014.
Article in Chinese | WPRIM | ID: wpr-345552

ABSTRACT

High temperature and humidity stress during seed growth and development of spring soybean can result in seed deterioration in South China. We isolated two genes (GmSBP and GmSBPL) encoding putative SBP proteins from soybean (Glycine max (L.) Merr.) to study their biological functions and response to abiotic stress,. The two SBP proteins are hydrophilic and incomplete membrane ones. Real-time quantitative (RT-PCR) analysis reveals that the expression of the two genes in the developing seeds of the seed deterioration resistant cultivar Xiangdou No. 3 and sensitive cultivar Ningzhen No. 1 was significantly affected by high temperature and humidity treatment. Meanwhile, the levels of sucrose and soluble sugar in the developing seeds of both cultivars were also affected under high temperature and humidity stress. During seed growth and development, the expression of the two genes as well as the levels of sucrose and soluble sugar reached the highest at 30 days after flower. GmSBP2 and GmSBPL were found to be differentially expressed in different soybean tissues. Sub-cellular localization indicated that two genes were located in cytoplasm and cell membrane. Our results indicate that GmSBP2 and GmSBPL might be involved in the response to abiotic stress, which will enrich our understanding of pre-harvest seed deterioration and resistance in soybean from one side.


Subject(s)
China , Gene Expression Regulation, Plant , Genes, Plant , Membrane Transport Proteins , Genetics , Plant Lectins , Genetics , Real-Time Polymerase Chain Reaction , Seeds , Soybean Proteins , Genetics , Soybeans , Genetics , Stress, Physiological
13.
Article in Korean | WPRIM | ID: wpr-147387

ABSTRACT

The vomeronasal organ (VNO) plays an important role in reproduction and social activities in ruminants including goats. A morphological study on the structure of VNO and its epithelial cells was carried out in Korean black goats. Grossly, the VNO of Korean goats opens into mouth through incisive ducts. Microscopically, the epithelium of VNO consisted of medial sensory epithelium and lateral non-sensory epithelium. Several blood vessels and nerve bundles were observed in the lamina propria encased by vomeronasal cartilage. Immunohistochemical staining showed that protein gene product (PGP) 9.5 was immunostained in the receptor cells of the sensory epithelium and in some cells of the non-sensory epithelium. Galectin-3 was mainly observed in the supporting cells of sensory and non-sensory epithelium. Lectins including wheat germ agglutinin, Ulex europaeus agglutinin, Bandeiraea simplicifolia lectin Isolectin B4, Dolichos biflorus agglutinin and soybean agglutinin used in this study were bound in VNO sensory, non-sensory epithelia as well as in the lamina propria with varying intensity. Collectively, this is a first descriptive morphological study of VNO of Korean black goat with special reference to lectin histochemistry.


Subject(s)
Blood Vessels , Cartilage , Dolichos , Epithelial Cells , Epithelium , Galectin 3 , Goats , Immunohistochemistry , Lectins , Mouth , Mucous Membrane , Plant Lectins , Reproduction , Ruminants , Soybean Proteins , Soybeans , Triticum , Ulex , Vomeronasal Organ
14.
Laboratory Animal Research ; : 113-126, 2013.
Article in English | WPRIM | ID: wpr-13110

ABSTRACT

In order to investigate the effects of a fermented soybean product (Chungkookjang, CKJ) on nerve growth factor (NGF) metabolism, NGF secretion ability and its related signaling pathway were analyzed in B35 neuronal cells and the Tg2576 mouse model of Alzheimer's disease (AD). In B35 cells, the concentration of NGF significantly increased upon treatment with Taegwang (TG)-CKJ and Shinhwa (SH)-CKJ extracts compared with vehicle. Further, a significant increase in PC12 cell length as well as the phsophorylation levels of TrkA and Akt, which are members of a high affinity NGF receptor signaling pathway, were observed after treatment with TG-CKJ and SH-CKJ conditional medium (CM). On the other hand, there was no difference in activation of the NGF receptor p75NTR signaling pathway between vehicle and all CKJ treated groups. In Tg2576 mice showing early stage of AD, the concentrations of NGF in the serum and brain were reduced compared with those in Non-Tg mice. Treatment of Tg2576 mice with SH-CKJ, which contains high concentrations of total flavonoids and phenolic compounds, for 8 weeks dramatically recovered the NGF level to that of Non-Tg mice. Furthermore, the low phosphorylation levels of TrkA and Erk in the NGF receptor TrkA signaling pathway were rapidly recovered to those of Non-Tg mice after SH-CKJ treatment in vehicle treated Tg2576 mice, whereas the phosphorylation level of Akt was maintained at a constant level. These results suggest that CKJ may stimulate NGF secretion ability as well as the NGF receptor TrkA signaling pathway in PC12 cells and Tg2576 mice.


Subject(s)
Alzheimer Disease , Animals , Brain , Flavonoids , Hand , Isoflavones , Mice , Nerve Growth Factor , Neurons , PC12 Cells , Phenol , Phosphorylation , Receptor, Nerve Growth Factor , Soybean Proteins , Soybeans
15.
Arq. bras. med. vet. zootec ; 64(5): 1085-1093, out. 2012. tab
Article in Portuguese | LILACS | ID: lil-655876

ABSTRACT

Quarenta e três ovelhas foram distribuídas em quatro tratamentos em um delineamento de blocos casualizados. Os tratamentos consistiram no fornecimento de dietas de flushing, formuladas com farelo de soja (FFS) ou com glúten de milho e farelo de algodão (FGM+FA) por 28 dias antes da ovulação, seguido pelo fornecimento de dietas de flushing ou de uma dieta de mantença (DM) durante 28 dias após a ovulação: T1 - FFS antes e após a ovulação (n=12); T2 - FFS antes da ovulação e DM após (n=11); T3 - FGM+FA antes e após a ovulação (n=10); T4 - FGM+FA antes da ovulação e DM após (n=10). O ganho de peso e o peso final não diferiram (P>0,05) entre os animais dos tratamentos. O ECC final foi maior (P<0,05) nas ovelhas do tratamento T3 do que nas do tratamento T2. A concentração de N-ureico foi maior (P<0,05) nas ovelhas dos tratamentos T1 e T3 do que naquelas dos tratamentos T2 e T4. A taxa de gestação e a prolificidade não diferiram (P>0,05) entre os animais. As dietas de flushing antes da ovulação, seguidas pelo fornecimento destas mesmas dietas ou de uma dieta de mantença após a ovulação, não alteraram a taxa de gestação e a prolificidade.


Forty-tree ewes were assigned to four treatments in a randomized block design. The treatments consisted of two flushing diets, composed of soybean meal (SMF) or corn gluten and cottonseed meal (CG+CMF) furnished for 28 days before ovulation, and feeding with flushing diets or a maintenance diet (MD) for 28 days after ovulation: T1 - (SMF) before and after ovulation (n=12); T2 - SMF before ovulation and a MD after (n=11); T3 - CG+CMF before and after ovulation (n=10); T4 - CG+CMF before ovulation and MD after (n=10). The gain in BW and the final BW did not differ (P>0,05) between treatments. The final BC was higher (P>0,05) in ewes in T3 treatment than in those in treatment T2. The urea N concentration was higher (P<0,05) in ewes in treatments T1 and T3 than those in treatments T2 and T4. The pregnancy rate and prolificacy did not differ (P>0,05) between treatments. The flushing diets before ovulation and feeding with this same diet or a maintenance diet after ovulation did not alter the pregnancy rate and prolificacy.


Subject(s)
Animals , Ovulation , Sheep/metabolism , Pregnancy Rate , Animal Feed/analysis , Rumen/metabolism , Animal Nutritional Physiological Phenomena , Glutens/analysis , Soybean Proteins/analysis
16.
Rev. chil. nutr ; 39(3): 52-57, set. 2012. graf, tab
Article in Spanish | LILACS | ID: lil-657708

ABSTRACT

The aim of this study was to evaluate the detection of soy proteins in raw and cooked meat products using SDS-PAGE and immunochemical methods. Ten raw model systems of bovine meat added with soy protein isolates, eight cooked model systems of boneless ham added with soy protein isolates and eight commercial meat products were analized. Model systems and commercial samples were analyzed by SDS-PAGE, Dot Blot, Immunoblotting and ELISA (Soy allergen kit from Neogen). Dot blot and Immunoblotting resulted to be the most sensitive analytical methods for the detection of soy proteins. The concentrations of the soy protein isolate obtained with the ELISA kit were much lower than real values. Soy proteins that were not declared in the label of several samples were detected using different methods. The heat treatment applied to the cooked meat products would affect the solubility of soy proteins and their ability to react with the antibodies of the ELISA kit used.


Se evaluó la detección de proteínas de soja en productos cárnicos crudos y cocidos utilizando SDS-PAGE y métodos inmunoquímicos. Se analizaron diez sistemas modelo de mezclas de carne vacuna con agregado de aislado de soja, ocho sistemas modelo de fiambres de cerdo con agregado de aislado de soja y ocho muestras comerciales. Los sistemas modelo y las muestras fueron analizadas por SDS-PAGE, Dot blot, Inmunoblotting y ELISA soja de Neogen. Dot Blot e Inmunoblotting resultaron los métodos más sensibles para la detección de proteínas de soja. Con el método de ELISA se obtuvieron valores de concentraciones de aislado de soja muy inferiores a los valores reales. En varias muestras comerciales se detectaron con los distintos métodos proteínas de soja no declaradas en los respectivos rótulos. El tratamiento térmico aplicado en los productos cocidos afectaría la solubilidad de las proteínas de soja y su capacidad para reaccionar con los anticuerpos presentes en el kit de ELISA utilizado.


Subject(s)
Enzyme-Linked Immunosorbent Assay , Determination , Soybean Proteins , Electrophoresis, Polyacrylamide Gel , Meat Products
17.
Iranian Journal of Public Health. 2012; 41 (4): 9-18
in English | IMEMR | ID: emr-128576

ABSTRACT

To examine the effects of soy [in the form of textured soy protein [TSP] and soy-nut] on body composition in elderly women with metabolic syndrome [MetS]. A 12-week randomized clinical trial was conducted on 75 women between 60-70 years of age with MetS in rural health clinics around Babol, Iran in 2009. The participants were randomly assigned to one of the three groups of soy-nut [35g/d], TSP [35g/d] and control. Body fat, lean mass and anthropometric indicators were measured before and after intervention, too. Participants were classified as overweight and showing android fat distribution. After 12 weeks of intervention, both soy-nut and TSP groups showed an increase of non-significant in lean mass [0.9 and 0.7 kg], hip circumference [0.45 and 0.28 cm], triceps skinfold [TSF] thickness [0.87 and 0.67mm] and reduction in BMI [-0.15 and -0.33], waist circumference [-0.83 and -1.2] and body fat [-1.5% and -1.7%]. Significant increase in the mean change of TSF and lean mass was observed in the users of soy-nut compared to the control group [P<0.01, P<0.05]. 12-week intervention of soy had a mild favorable effect on body composition in elderly women with MetS


Subject(s)
Humans , Female , Body Composition , Metabolic Syndrome , Aged , Soybean Proteins , Adipose Tissue , Anthropometry
18.
Article in English | WPRIM | ID: wpr-819790

ABSTRACT

OBJECTIVE@#To clarify the effect of soy isoflavones on prevention of osteoporosis, and the effective dosage of soy isoflavones and its duration.@*METHODS@#Random control trials that investigated the association of soy isoflavones and osteoporosis were included in the meta-analysis by researching MEDLINE, EMBASE and the Chinese Biomedical Database up to October 2011. The Rev Man software was used for all of the statistical analysis.@*RESULTS@#The present meta-analysis found that soy isoflavones significantly increased the bone mineral density by 54% and decreased the bone resorption marker urinary deoxypyridinoline (DPD) by 23% compared to baseline in women. Using random effects model, the effect of isoflavones on bone mineral density (BMD) regarding menopausal status and isoflavone dose revealed higher weighted mean difference changes were found in postmenopausal women and isoflavone dose above 75 mg/d. Subgroup analysis of trials with menopausal status, supplement type, isoflavone dose and intervention duration that used soy isoflavone extracts resulted in significant different overall effect of DPD using by random effects model. Sensitivity analysis indicated that the effect of soy isoflavones on BMD and DPD was robust.@*CONCLUSIONS@#The present meta-analysis reveals that soy isoflavone supplements significantly increase bone mineral density and decrease the bone resorption marker urinary DPD. It shows no significant effect on bone formation markers serum bone alkaline phosphatase. The significant effect of soy isoflavones on BMD and urinary DPD is relative to menopausal status, supplement type, isoflavone dose and intervention duration.


Subject(s)
Amino Acids , Metabolism , Bone Density , Physiology , Bone Resorption , Dietary Supplements , Female , Humans , Isoflavones , Middle Aged , Osteoporosis, Postmenopausal , Diet Therapy , Randomized Controlled Trials as Topic , Soybean Proteins
19.
Rev. Inst. Adolfo Lutz ; 70(3): 311-315, set. 2011. tab, graf
Article in English | LILACS, SES-SP, SESSP-CTDPROD, SES-SP, SESSP-ACVSES, SESSP-IALPROD, SES-SP, SESSP-IALACERVO | ID: lil-644378

ABSTRACT

Soy proteins are widely employed in meat products. However addition of non-meat proteins in calabresa sausages is not allowed according to the Brazilian legislation and in case of the non-declared addition of this foreign protein in consumed food, it may trigger allergic reactions in some consumers. Polyacrylamide gel electrophoresis (SDS-PAGE) was used for determining soy proteins in calabresa sausages. Fraud simulations were performed adding different concentrations (0%; 0.5%; 1%; 2%; 5%;10%; 20% and 100%) of soy proteins in sausages. The qualitative analysis was not sensitive to detect thelo west concentrations of soy proteins. On the other hand, by using semi-quantitative analysis by means of densitometry of selected protein fractions from soy and porcine meat, the presence of soy proteins could be determined in the all of analyzed concentrations. This methodology could be implemented, without large investments, for conducting quality control of sausages.


Subject(s)
Densitometry , Electrophoresis, Gel, Two-Dimensional , Meat Products , Soybean Proteins
20.
Rev. nutr ; 24(1): 161-172, jan.-fev. 2011. graf, tab
Article in English | LILACS | ID: lil-588217

ABSTRACT

This study evaluated the effects of soy protein containing isoflavones on the lipid profile of women. A meta-analysis including 13 eligible randomized controlled trials was carried out. The literature was systematically searched for randomized controlled trials on the effects of soy protein containing isoflavones on the serum lipids of adult women. The main searched databases were PubMed, Cochrane Library, MedLine, Lilacs and Web of Science. Randomized controlled trials were included if they met the following criteria: published from 1966 to 2005, the study population consisted of women only, had either a crossover or a parallel design and the amounts of soy protein and isoflavones consumed were provided. Weighted mean effect sizes were calculated for net changes in serum lipid concentrations using fixed-effects and random-effects models. Prespecified subgroup analyses were performed to explore the influence of covariates on net lipid change. Soy protein with isoflavones was associated with a significant decrease in total serum cholesterol (by 5.34mg/dL, or 2.4 percent, p=0.03). No significant associations were detected for low density lipoprotein-cholesterol, triacylglycerols and high density lipoprotein-cholesterol. Amounts of soy protein greater than 40g decreased total cholesterol by 6.56mg/dL (95 percent CI: -12.35 to -0.39, p=0.04). Soy protein supplementation had small statistically significant effects on the total serum cholesterol of women, but they were clinically insignificant. Furthermore, there were no statistically significant effects on serum low density lipoprotein-cholesterol, high density lipoprotein-cholesterol or triglycerides.


Avaliar o efeito da proteína de soja isolada contendo isoflavonas sobre os níveis de lipídeos séricos em mulheres adultas através de uma metanálise. Realizou-se uma busca sistemática de artigos nos bancos de dados Cochrane Library, MedLine e Web of Science databases. Foram selecionados somente ensaios clínicos randomizados que apresentavam os seguintes critérios: desenho do estudo paralelo ou crossover, publicados entre 1966 e 2005, sujeitos do sexo feminino, especificação da quantidade administrada de proteína de soja isolada contendo isoflavonas. Após criteriosa seleção e análise da qualidade dos estudos, conduziu-se uma metanálise incluindo 13 ensaios clínicos randomizados. O efeito do consumo de proteína de soja isolada sobre os níveis de lipídeos séricos foi calculado utilizando-se os modelos de efeito fixo e efeitos aleatórios. Realizou-se análise em subgrupos para avaliar a influência de covariáveis sobre alterações dos níveis de lipídeos sanguíneos. A metanálise mostrou que o consumo de proteína de soja isolada está associado à diminuição do colesterol-total (-5,34mg/dL, ou 2.4 por cento, p=0,03). Não se encontraram alterações significativas ao avaliar o lipoproteina de baixa densidade-colesterol, os triglicerídeos e o lipoproteina de alta densidade-colesterol. O consumo de proteína de soja isolada, em quantidades maiores ou iguais a 40g/dia, reduziu os níveis de colesterol-total em 6,56mg/dl (95 por cento IC: -12,35 a 0,39, p=0,04). Os achados sugerem efeitos estatisticamente significativos de pequena magnitude sobre os níveis de lipídeos séricos em mulheres, mas clinicamente insignificativos. Além disso, não foram encontrados efeitos estatisticamente significativos sobre a concentração sérica de lipoproteina de baixa densidade-colesterol, lipoproteina de alta densidade-colesterol ou triglicerídios.


Subject(s)
Humans , Female , Adult , Anticholesteremic Agents/analysis , Soybean Proteins/metabolism , Soybean Proteins/therapeutic use
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