ABSTRACT
La contaminación por arsénico del agua de consumo humano, es un problema de salud pública, porque produce diversas enfermedades cancerígenas y de piel. Objetivo. Determinar niveles de arsénico en el agua de pozos, y evaluar el grado de conocimiento y actitud sobre el consumo del agua familiar. Materiales y métodos. Se utilizaron 96 muestras del agua de pozos del distrito de Juliaca. Las cuales se analizaron en laboratorio de Unidad de Servicios de Análisis Químicos de la Facultad de Química - Universidad Nacional Mayor de San Marcos-Lima, mediante la técnica Espectrofotometría de Absorción Atómica con Horno de Grafito. Los datos de arsénico fueron procesados estadísticamente mediante el diseño completamente al azar. Asimismo, la descripción del conocimiento y actitud del consumo de agua familiar se realizó aplicando la técnica de la encuesta y el instrumento fue un cuestionario de 11 ítems para la variable conocimiento y 7 para la variable actitud, con respuestas de alto, medio y bajo para conocimiento y buena, regular y mala calificadas con escala de Likert. Resultados. La concentración promedio fue 0.031 mg de As/L de agua y entre zonas hubo semejanza (p>0.05). En el grado de conocimiento sobre contaminación con arsénico en el agua de consumo humano respondieron el 40.81 % con calificación alta y el 59.19% están entre medio y bajo; en actitud, la calificación buena obtuvo menos del 50 % de encuestados y el resto están entre regular y mala. Conclusiones. El contenido arsenical en el agua de pozos supera los límites máximos permisibles según Organización Mundial de Salud y más del 50% se exponen al agua contaminada.
Arsenic contamination of drinking water is a public health problem, because it causes various carcinogenic and skin diseases. Objective. To determine arsenic levels in well water, and to evaluate the degree of knowledge and attitude about family water consumption. Materials and methods. Ninety-six samples of well water from the district of Juliaca were used. These were analyzed in the laboratory of the Chemical Analysis Services Unit of the Faculty of Chemistry - Universidad Nacional Mayor de San Marcos-Lima, using the technique Atomic Absorption Spectrophotometry with Graphite Furnace. The arsenic data were statistically processed using a completely randomized design. Likewise, the description of the knowledge and attitude of family water consumption was carried out by applying the survey technique and the instrument was a questionnaire of 11 items for the knowledge variable and 7 for the attitude variable, with answers of high, medium and low for knowledge and good, regular and bad rated with a Likert scale. Results. The average concentration was 0.031 mg As/L water and there was similarity between zones (p>0.05). In the degree of knowledge about arsenic contamination in drinking water, 40.81% responded with high qualification and 59.19% were between medium and low; in attitude, the good qualification obtained less than 50% of respondents and the rest were between regular and bad. Conclusions. The arsenic content in well water exceeds the maximum permissible limits according to the World Health Organization and more than 50% are exposed to contaminated water.
A contaminação da água potável por arsênico é um problema de saúde pública, pois causa várias doenças de pele e carcinogênicas. Objetivo. Determinar os níveis de arsênico na água de poço e avaliar o grau de conhecimento e atitude em relação ao consumo doméstico de água. Materiais e métodos. Foram utilizadas 96 amostras de água de poço do distrito de Juliaca. Elas foram analisadas no laboratório da Unidade de Serviços de Análises Químicas da Faculdade de Química da Universidade Nacional Mayor de San Marcos-Lima, usando a técnica de Espectrofotometria de Absorção Atômica com Forno de Grafite. Os dados sobre arsênico foram processados estatisticamente usando um desenho completamente aleatório. Da mesma forma, a descrição do conhecimento e da atitude do consumo familiar de água foi realizada aplicando a técnica de pesquisa e o instrumento foi um questionário de 11 itens para a variável conhecimento e 7 para a variável atitude, com respostas de alto, médio e baixo para o conhecimento e classificado como bom, regular e ruim em uma escala Likert. Resultados. A concentração média foi de 0,031 mg As/L de água e houve similaridade entre as zonas (p>0,05). Quanto ao grau de conhecimento sobre a contaminação por arsênico na água potável, 40,81% responderam com uma pontuação alta e 59,19% ficaram entre médio e baixo; quanto à atitude, a pontuação boa foi obtida por menos de 50% dos entrevistados e o restante ficou entre regular e ruim. Conclusões. O teor de arsênico na água de poço excede os limites máximos permitidos de acordo com a Organização Mundial da Saúde e mais de 50% estão expostos à água contaminada.
Subject(s)
Spectrophotometry, Atomic , Spectrum Analysis/methodsABSTRACT
El objetivo fue caracterizar el contenido de sodio en preparaciones de consumo frecuente fuera del hogar en tres zonas de Lima Metropolitana. Se realizó un estudio transversal para el cual se identificaron veinte preparaciones de consumo frecuente según el lugar de expendio. La determinación del contenido de sodio se obtuvo mediante espectroscopía de absorción atómica en preparaciones recolectadas en el 2019. La mediana del contenido de sodio en productos de venta ambulatoria fue 492,36 mg/100 g (RIQ: 83,93 - 918,78); 471,37 mg/100 g, en preparaciones tradicionales y típicas (RIQ: 76,04 - 765,39); y 471,06 mg/100 g (RIQ: 115,31 - 695,18), en comidas rápidas. El 65% de las preparaciones fueron consideradas altas en sodio según los parámetros peruanos, mientras que el 30% de las preparaciones también presentaron un alto contenido, de acuerdo con los parámetros del Reino Unido. La mayoría de las preparaciones expendidas y consumidas fuera del hogar presentan un elevado contenido de sodio. Es esencial involucrar a todos los actores que participan en la preparación de alimentos para el consumo fuera del hogar, con el fin de sensibilizarlos e incluirlos en la promoción de políticas enfocadas en la reducción del consumo de sodio.
The aim of this study was to characterize the sodium content in commonly consumed away-from-home food in three areas of Metropolitan Lima. We conducted a cross-sectional study, in which twenty frequently consumed foods were identified according to the place of sale. Sodium content was determined through atomic absorption spectroscopy in preparations collected in 2019. The median sodium content in street food products was 492.36 mg/100g (IQR: 83.93 - 918.78), 471.37 mg/100 g in artisanal food (IQR: 76.04 - 765.39) and 471.06 mg/100 g in fast food (IQR: 115.31 - 695.18). Sixty-five percent of the foods were classified as having high sodium content according to Peruvian regulations, while 30% of the preparations had high sodium content, according to UK parameters. Most food sold and consumed away from home have high sodium content. It is essential to engage all stakeholders involved in food preparation for away-from-home consumption in order to raise awareness and involve them in the promotion of policies aimed at reducing sodium intake.
Subject(s)
Spectrophotometry, AtomicABSTRACT
Objective: Compare the pH values and calcium ion release of calcium hydroxide-based liner materials before and after light-curing. Material and Methods: The materials evaluated were: hydrox-cal white (HW), hydrox-cal dentin (HD), Biocal (BC) and UltraBlend Plus (UB). 120 samples of the liner materials were inserted into a PVC tube (n=15). The samples from HW+A, HD+A, BC+A and UB+A were subjected to photoactivation. The other groups HW+N, HD+N, BC+N and UB+N were only inserted in a glass tube with deionized water. The pH was measured 24 hours and 14 days after the inclusion of the samples with the aid of a pH meter. The calcium release was analyzed with the aid of an atomic absorption spectophotometer at 24h and 14 days. The results were submitted to the Shapiro-Wilk test, followed by ANOVA and Tukey test (p=0.05). Results: In 24h, the groups that were not light cured showed the highest pH values (p<0.05). In 14 days, BC+N and BC+A demonstrated the lowest pH values. The groups that were not light cured also showed higher calcium release values in 24h and 14 days (p<0.05). Conclusion: Photoactivation of calcium hydroxide-based liner materials negatively interferes with calcium ion release, as well as with pH.(AU)
Objetivo: Comparar os valores de pH e liberação de íons cálcio de materiais forradores à base de hidróxido de cálcio antes e depois da fotopolimerização. Material e métodos: Os materiais avaliados foram: Hidrox-cal branco (HW), Hidrox-cal dentina (HD), Biocal (BC) e UltraBlend Plus (UB). 120 amostras dos materiais de revestimento foram inseridas em um tubo de PVC (n=15). As amostras de HW +A, HD+A, BC+A e UB+A foram submetidas à fotoativação. Os demais grupos HW +N, HD+N, BC+N e UB+N foram inseridos apenas em um tubo de vidro com água deionizada. O pH foi medido 24 horas e 14 dias após a inclusão das amostras com o auxílio de um medidor de pH. A liberação de cálcio foi analisada com o auxílio de um espectrofotômetro de absorção atômica em 24h e 14 dias. Os resultados foram submetidos ao teste de Shapiro-Wilk, seguido de ANOVA e teste de Tukey (p=0,05). Resultados: Em 24h, os grupos não fotopolimerizados apresentaram os maiores valores de pH (p<0,05). Em 14 dias, BC+N e BC+A apresentaram os menores valores de pH. Os grupos não fotopolimerizados também apresentaram maiores valores de liberação de cálcio em 24h e 14 dias (p<0,05). Conclusão: A fotoativação de materiais de revestimento à base de hidróxido de cálcio interfere negativamente na liberação de íons cálcio e no pH (AU)
Subject(s)
Humans , Calcium Hydroxide/chemistry , Light-Curing of Dental Adhesives , Hydrogen-Ion Concentration , Spectrophotometry, Atomic , Materials Testing , Dental Restoration, PermanentABSTRACT
Abstract Objective: To compare the high-cycle fatigue behavior of four commercially available NiTi orthodontic wires. Material and Methods: Twelve NiTi orthodontic wires, round, 0.016-in, three per brand, were selected and divided into four groups: G1 - Heat-activated NiTi, G2 - Superelastic NiTi, G3 - Therma-Ti, and G4 - CopperNiTi. The atomic absorption spectrometry method was used to determine the chemical composition of investigated NiTi wires. We also performed a fatigue test at three-point bending using a universal testing machine for 1000 cycles in a 35 °C water bath. For the first and thousandth cycle, the average plateau load and the plateau length were determined in the unloading area of the force versus displacement diagram. In addition, we calculated the difference between the average plateau load of the first and thousandth cycle (∆F), as well as the difference between the plateau length of both cases (∆L). Results: According to our results, there were no significant differences between the average plateau load of the first and thousandth cycles of each group (p>0.05) and in the plateau length of the first and thousandth cycles of the groups (p>0.05). Conclusion: There were no significant differences between the groups changing the superelasticity property after high-cycle fatigue.
Subject(s)
Orthodontic Wires , Orthodontics , Stress, Mechanical , Dental Instruments , Spectrophotometry, Atomic/instrumentation , Spectrum Analysis/methods , In Vitro Techniques/methods , Materials Testing , Analysis of VarianceABSTRACT
Abstract Recent studies have demonstrated that 30% of the world's population suffers of anemia, half of the cases are related to iron deficiency, and the most common treatment is the use of iron supplementation. In this framework, the iron and zinc determination from different dietary supplements was performed by flame atomic absorption spectrophotometry. Concerning the dissolution of supplements, direct acid dissolution, wet digestion, and microwave digestion (MW) techniques were used for sample preparation. The iron and zinc recovery results demonstrated that the MW technique was the most appropriate for all of the supplements with the highest metal recovery yields. Moreover, the method validation parameters referred to a linear range for iron of 0.1-4 mg L-1 with a regression coefficient (R2) of 0.9998 ± 0.002, while for zinc it was 0.01-1 mg L-1 (R2 = 0.9997 ± 0.003). The limit of detection and quantification values were calculated as 0.03 and 0.09 mg L-1 for iron and 0.01 and 0.02 mg L-1 for zinc, respectively. The accuracy of the method was evaluated from the % recovery yield for iron and zinc, which, respectively, resulted in an oscillate of 99.2% to 102%, and 99.4% to 100.4% for the dietary investigated supplements. The precision of the method was determined by intra-day and inter-day precision with a relative standard deviation that was <2.0%
Subject(s)
Spectrophotometry, Atomic/methods , Zinc/analysis , Dietary Supplements/classification , Iron/analysis , Validation Study , Anemia/pathologyABSTRACT
RESUMEN La contaminación por metales pesados en los ecosistemas acuáticos en el planeta es una de las más severas problemáticas que comprometen la seguridad alimentaria a nivel local, regional y global. En este estudio, se aborda el problema específico de la bioconcentración de mercurio (Hg), plomo (Pb) y Zinc (Zn) en los tejidos orgánicos de 2 de las especies de peces (Ariopsis felis y Diplodus annularis) de mayor importancia alimenticia para los pobladores rivereños del río Ranchería, al norte de Colombia. Las muestras de tejido orgánico se sometieron a una digestión ácida (HNO3 y HClO 4 en relación 3:1) y la cuantificación de los metales se realizó por espectrofotometría de absorción atómica. Adicionalmente, se midieron los niveles de los metales en el sedimento, con el objeto de determinar índices de calidad medioambientales, lo que permitió determinar que los metales pesados no presentan riesgo para los organismos acuáticos que habitan en esta zona. Se encontraron diferentes valores de concentración de metales pesados: en orden decreciente, Zn > Hg > Pb, en sedimento, y Hg > Zn > Pb, en el tejido biológico. Los resultados muestran que las concentraciones de los metales pesados presentes en el tejido de los peces y en el sedimento guardan una correlación positiva y una regresión lineal estadísticamente significativa (p = 0,001, p < 0,05). La presencia de metales pesados en el sedimento del río y de bioconcentrados en los peces podría derivarse de fuentes puntuales y difusas relacionadas con actividades agrícolas, pecuarias, mineras y aguas residuales.
ABSTRACT Heavy metal pollution in aquatic ecosystems in the area is one of the most severe problems that compromise food security at the local, regional and global levels. In this study, the specific problem of the bioconcentration of mercury (Hg), lead (Pb), and Zinc (Zn) in 2 of the higher nutritional importance fish species (Ariopsis felis and Diplodus annularis) for the riverside inhabitants of the Ranchería river, in northern Colombia, is addressed. The organic tissue samples were subjected to acid digestion (HNO3 and HClO4 in a 3:1 ratio), and the quantification of metals was carried out by atomic absorption spectrophotometry. Additionally, the levels of metals in the sediment were measured, in order to determine environmental quality indices. This allow to determine that heavy metals do not present a risk to the aquatic organisms that inhabit this area. Different heavy metal concentration values were found: in decreasing order, Zn> Hg> Pb in sediment, and Hg> Zn> Pb in biological tissue. The results reveal that the concentrations of heavy metals present in fish tissue and sediment show a positive correlation, and a statistically significant linear regression (p = 0,001, p < 0,05). The presence of heavy metals in river sediment and bioconcentrates in fish could derive from point and diffuse sources related to agricultural, livestock, mining and wastewater activities.
Subject(s)
Animals , Spectrophotometry, Atomic , Metals, Heavy , Environmental Pollution , Fishes , Bioaccumulation , Mining , Zinc , Risk , Ethics Committees , Lead , MercuryABSTRACT
Objetivo: Avaliar a Resistência de União (RU), Grau de Conversão (GC), Liberação de Cálcio (LC), Resistência Flexural (RF), Módulo de Elasticidade (ME) e Viscosidade (VS) de cimentos experimentais à base de cálcio autocondicionantes e autoadesivos. Três cimentos experimentais: A (CONTROLE) sem fonte de cálcio, B (HIDROCAL) com hidróxido de cálcio, C (CLORECAL) com cloreto de cálcio foram sintetizados e avaliados juntamente a um cimento comercial D (ULTRAPLUS) (Ultra-Blend Plus®). Metodologia: Para a RU, foi utilizado o teste de cisalhamento, cujas amostras (1,3mm de diâmetro com 2mm de altura) foram aderidas à dentina bovina (n=10) e tensionadas em máquina de ensaios (1 mm/min) atéÌ ocorrer o deslocamento. Para o GC (n=3), foi utilizada a Espectroscopia Infravermelha com transformada de Fourier (FTIR). Na determinação da LC (n=3) foram utilizadas amostras circulares (1,0 mm x 0,4 mm) e a leitura foi realizada através de um espectrofotômetro de absorção atômica nos tempos de 3h, 24h e 72h. Para RF/ME (n=10), amostras em formato de barra (7mm x 2mm x 1mm) foram confeccionadas e avaliadas por meio de uma Máquina de Ensaios Universal no teste de flexão de três pontos. Para a VS (n=30), a variação da taxa de deformação foi determinada por um reômetro rotacional de cilindros concêntricos. Os dados foram analisados por ANOVA 1 e 2 fatores seguido pelo pós teste de tukey (p<0,05). Resultados: Para RU todos os materiais experimentais apresentaram valores estatisticamente maiores que o comercial (p<0,01). Para o GC os cimentos CONTROLE e HIDROCAL apresentaram valores estatisticamente maiores que os demais (p<0,01). Frente à LC o CLORECAL e ULTRAPLUS apresentaram valores estatisticamente superiores após 72h (p<0,01). O ULTRAPLUS apresentou estatisticamente a maior RF e o menor ME (p<0,05). Os cimentos HIDROCAL e CLORECAL apresentaram valores de VS estatisticamente superiores aos demais (p<0,01). Conclusão: De maneira geral, o cimento experimental à base de cloreto de cálcio mostrou-se mais promissor (AU).
Objective: To evaluate the Bond Strength (BS), Degree of Conversion (DC), Calcium Release (CR), Flexural Strength (FS), Elastic Modulus (EM) and Viscosity (VS) of experimentally calcium based cements self-etching and self-adhesive. Three experimental cements: A (CONTROL), B (HYDROCAL), C (CLORECAL) were synthesized and evaluated with a commercial cement D (ULTRAPLUS) (Ultra-Blend Plus®- South Jordan, Utah, USA). Methodology: For the BS, the shear test was used, whose samples 1.3mm in diameter and 2mm in height) were adhered to the bovine dentin (n=10) and tensioned in a testing machine (1mm/min) until occurring the displacement. For the DC (n=3), Infrared Spectroscopy with Fourier transform (FTIR) was used. In determining the CR (n = 3) circular samples (1.0mmx0.4 mm) were used and the reading was performed through an atomic absorption spectrophotometer at 3h, 24h and 72h. For FS/ ME (n= 0), bar-shaped samples (7mmx 2mmx1mm) were made and evaluated using a Universal Testing Machine in the three-point flexion test. For VS (n = 30), the variation in the strain rate was determined by a rotational concentric cylinder rheometer. Results: For BS, all experimental materials showed statistically higher values than the commercial material (p <0.01). For the DC, the CONTROL and HIDROCAL cements showed statistically higher values than the others (p <0.01). In relation to CR, CLORECAL and ULTRAPLUS showed statistically higher values after 72 hours (p <0.01). ULTRAPLUS showed statistically the highest FS and the lowest EM (p <0.05). The HIDROCAL and CLORECAL cements showed VS values statistically higher than the others (p <0.01) Conclusion: In general, the experimental cement based on calcium chloride proved to be more promising (AU).
Subject(s)
Calcium Chloride , Calcium Hydroxide , Dental Cements , Dental Pulp Capping , Flexural Strength , Spectrophotometry, Atomic , In Vitro Techniques , Analysis of Variance , Spectroscopy, Fourier Transform Infrared , Fourier AnalysisABSTRACT
This study focuses on the heavy metals concentrations (Fe, Cu, Zn, Pb, and Cd) in certain common milk species are collected from Iraqi markets using Flame Atomic Absorption Spectrophotometer-6300 AA, Shimadzu, Japan, respectively. This study shows the pollution in the environment obtained by heavy metals. The results showed that Cr, Cd, Cu, Zn, and Fe were varying according to the order: Zn>Fe>Cr>Cd>Cu. levels of heavy metals were 0.610(Cr), 0.125(Cd), 0.052(Cu), and 6.902(Zn), and 0.759(Fe). All the heavy metals were observed within maximum limit in milk. Overall, the number of analyzed heavy metals and sample size were limited in present study. Keywords: Heavy metals; Milk; Najaf; Flame atomic absorption; Spectrophotometer This study focuses on the heavy metals concentrations (Fe, Cu, Zn, Pb, and Cd) in certain common milk species are collected from Iraqi markets using Flame Atomic Absorption Spectrophotometer-6300 AA, Shimadzu, Japan, respectively. This study shows the pollution in the environment obtained by heavy metals. The results showed that Cr, Cd, Cu, Zn, and Fe were varying according to the order: Zn>Fe>Cr>Cd>Cu. levels of heavy metals were 0.610(Cr), 0.125(Cd), 0.052(Cu), and 6.902(Zn), and 0.759(Fe). All the heavy metals were observed within maximum limit in milk. Overall, the number of analyzed heavy metals and sample size were limited in present study
Subject(s)
Spectrophotometry, Atomic , Flame Spectrophotometers , Metals, Heavy/toxicity , MilkABSTRACT
RESUMEN La leche humana materna tiene una composición única para proporcionar a los recién nacidos todos los nutrientes esenciales como proteínas, grasas, hidratos de carbono, minerales, vitaminas y otras substancias fisiológicamente activas, con el fin de suplir en cantidad y calidad las necesidades calóricas y nutricionales del lactante. El objetivo fue establecer las concentraciones totales de cobre y zinc en la leche materna en sus distintas etapas: calostro, de transición y madura, determinadas espectrométricamente y la influencia del estado nutricional de madres lactantes; estudio descriptivo transversal en madres entre 18 y 35 años, sanas y no fumadoras; los resultados evidencian una disminución en las concentraciones de cobre y zinc a medida que la lactancia va evolucionando, además, el promedio de índice de masa corporal de las madres en período de lactancia reflejaron sobrepeso. Concluimos que el contenido calórico de los oligoelementos de la leche materna es esencial para el crecimiento de los niños por los beneficios nutricionales e inmunológicos que proporciona, por lo que es importante que la madre se encuentre en un estado nutricional adecuado que permita suministrar los requerimientos adecuados al niño para su crecimiento y desarrollo.
ABSTRACT Human milk has a unique composition and provides newborns with all essential nutrients such as proteins, fats, carbohydrates, minerals, vitamins and other physiologically active substances, in order to supply in quantity and quality the caloric and nutritional needs of the infant. The objective of this study was to establish the total concentration of copper and zinc in breast milk in its different stages: colostrum, transitional and mature milk, determined spectrometrically and the influence of the nutritional status of nursing mothers. We conducted a descriptive cross-sectional study in healthy non-smoking mothers between 18 and 35 years of age. Lower concentrations of copper and zinc as lactation progressed were observed. The average of body mass index of nursing mothers reflected overweight. We concludes that the caloric content of the trace elements of breast milk is essential for the growth of children because of the nutritional and immunological benefits that it provides, so it is important that the mother has an adequate nutritional state that allows to supply the adequate requirements for the child to grow and develop.
Subject(s)
Humans , Female , Infant, Newborn , Adolescent , Adult , Young Adult , Zinc/analysis , Nutritional Status , Copper/analysis , Milk, Human/chemistry , Spectrophotometry, Atomic , Cross-Sectional StudiesABSTRACT
This study aimed to measure the concentrations of minerals in different regions and pigmentation of horses' hooves, mules and donkeys. A total of 20 Crioulo horses, 20 mules and 20 Pega donkeys were used. They formed two groups: pigmented (dark) or non-pigmented (bright) hooves. All animals were unshod and with adequate foot conformation. Females were non-pregnant and without foal and no animals were in training. Samples were collected from the hooves of all limbs in the regions of wall, sole and frog. The concentrations of calcium, magnesium, copper, iron and zinc were determined by atomic absorption spectrophotometry; sodium and potassium concentrations were determined by atomic emission spectrophotometry. Among the groups it was observed that the wall, sole and frog of donkeys' hooves presented higher concentrations of sodium and calcium and with the exception of the frog that also obtained higher levels of copper in this species. Mules had lower concentrations of zinc in the wall and the horses had higher concentrations of potassium and zinc in the sole and higher magnesium in the frog and wall. There was no difference (P>0.05) in concentrations regarding the pigmentation of the hooves. The different regions of the hooves in each group presented similar concentrations of minerals and it was regardless of the pigmentation. The mineral concentrations may be related to desirable functional properties of the donkeys' hooves. Pigmented and non-pigmented hooves have similar concentrations of minerals.
O objetivo desse trabalho foi mensurar os teores de minerais nas diferentes regiões e pigmentações dos cascos de equinos, muares e asininos. Utilizaram-se 20 equinos da raça Crioula, 20 muares e 20 asininos da raça Pêga. Formaram-se dois grupos conforme os cascos pigmentados (escuros) e não pigmentados (claros). Todos os animais estavam desferrados e com adequada conformação podal e as fêmeas estavam não gestantes e sem potro ao pé e nenhum animal estava em treinamento. Amostras foram coletadas dos cascos de todos os membros nas regiões de parede, sola e ranilha. As concentrações de cálcio, magnésio, cobre, ferro e zinco foram determinadas por espectrofotometria de absorção atômica e as concentrações de sódio e potássio foram determinadas por espectrofotometria de emissão atômica. Entre os grupos observou-se que a parede, sola e ranilha nos asininos apresentaram maiores valores de sódio e cálcio e, exceto para a ranilha, também obteve-se maiores teores de cobre nesta espécie. Os muares apresentaram menores teores de zinco na parede e, os equinos, maiores teores de potássio e zinco na sola e ranilha e de magnésio na ranilha e parede. Não houve diferença (P>0,05) nos teores dos minerais quanto à pigmentação dos cascos. As diferentes regiões dos cascos em cada grupo apresentaram teores similares de minerais e este independeu da pigmentação. As variações dos teores de minerais entre os grupos podem estar relacionados a propriedades funcionais desejáveis dos cascos dos asininos. Cascos pigmentados e não pigmentados apresentam teores similares dos minerais.
Subject(s)
Animals , Spectrophotometry, Atomic/veterinary , Equidae/anatomy & histology , Hoof and Claw/anatomy & histology , Horses , Minerals/analysis , PodiatryABSTRACT
RESUMEN Objetivos. Cuantificar la cantidad de titanio (Ti) en tejido respiratorio obtenidas por autopsia en personas que residieron en la Ciudad de México. Materiales y métodos. Se realizó un estudio exploratorio en el cual se obtuvieron 216 muestras de tejido respiratorio de lóbulos pulmonares y nódulos linfáticos peribronquiales, procedentes de autopsias médico legales de 36 cadáveres con antecedente de haber residido un mínimo de dos años en la Ciudad de México. Se realizaron análisis histopatológicos de las muestras y se cuantificó el Ti existente en ellas mediante plasma de inducción acoplado a la espectrofotometría de absorción atómica. Resultados. La cantidad de Ti en tejido respiratorio se distribuyó entre 0 microgramos de Ti en tejido seco (µg Ti/g ts) y 39,7 µg Ti/g ts, se identificó la presencia de Ti distribuido hasta en seis veces mayor cantidad en los nódulos linfáticos que en lóbulos pulmonares. El análisis de conglomerados mostró que la muestra estaba conformada por cuatro grupos de individuos agrupados de acuerdo a la cantidad y distribución del Ti en el tracto respiratorio bajo, a la edad, índice de masa corporal, lugar y tiempo de residencia en la ciudad de México. Conclusiones. Debido al patrón de distribución observado el Ti encontrado en las muestras de tejido respiratorio podría provenir del aire urbano, la cantidad de Ti en conjunto con otros elementos podría ser causante de patologías respiratorias.
ABSTRACT Objetivos. To quantify the levels of titanium (Ti) in respiratory tissue obtained by autopsy in people who resided in Mexico City. Materials and Methods. An exploratory study was conducted in which 216 samples of respiratory tissue from pulmonary lobes and peribronchial lymph nodes were obtained from legal medical autopsies of 36 corpses with a minimum of two years of residence in Mexico City. Histopathological analyses of the samples were performed and the Ti existing in them was quantified by induction plasma coupled to atomic absorption spectrophotometry. Results. The amount of Ti in respiratory tissue was distributed between 0 micrograms of Ti in dry tissue (µg Ti/g ts) and 39.7 µg Ti/g ts; the presence of Ti was identified as distributed in up to six times a higher quantity in the lymph nodes than in pulmonary lobes. Cluster analysis showed that the sample consisted of four groups of individuals grouped according to the amount and distribution of Ti in the lower respiratory tract, age, body mass index, place and time of residence in Mexico City. Conclusions . Due to the distribution pattern observed, Ti found in respiratory tissue samples may come from urban air; the amount of Ti in conjunction with other elements may be the cause of respiratory pathologies.
Subject(s)
Adolescent , Adult , Aged , Aged, 80 and over , Child , Child, Preschool , Female , Humans , Male , Middle Aged , Young Adult , Titanium/analysis , Lung/chemistry , Lymph Nodes/chemistry , Spectrophotometry, Atomic , Autopsy , Cluster Analysis , MexicoABSTRACT
Objective: To identify the exposure of lead through saliva and the presence of gingival pigmentation in the risk group. Material and Methods: The type of this research is analytic observational with cross-sectional study design. The sample consisted of 40 subjects, who were divided into two groups of 20 each: G1: Gasoline Fuel Station and G2: Officer in Dental Hospital. To determine the level of leads in saliva is measured by atomic absorption spectrophotometry and assessment of the severity of lead-gingival lead line in this study based on the area of the gingival surface based on the number of dental areas. The data were analyzed with the t-test and Chi-square test. The level of significance was set at 5%. Results: The average lead content in saliva group exposed was 6.66 µg / ml and control group 4.72 µg / ml (p≤0.05). There is a correlation between gingival lead line incidence with exposure to lead (OR = 3.33; p = 0.001). Conclusion: The identification of exposure to lead poisoning can be determined by examination in saliva and ginggiva state, it is proven that the risk of gingival lead (Ginggival Lead Line) in the worker group at the gas station station is 3.3 times more risk than the control group. Occupational safety factors are important for workers at high risk of exposure to the element of lead for additional education on the importance of using masks to prevent the severity of the occurrence of lead effects on overall health.
Subject(s)
Humans , Male , Female , Adult , Middle Aged , Saliva , Pigmentation , Diagnosis, Oral/methods , Filling Station/analysis , Indonesia , Lead/analysis , Spectrophotometry, Atomic/methods , Cross-Sectional Studies/methods , Risk FactorsABSTRACT
ABSTRACT Objective: It is proposed that in some conditions such as pregnancy and osteoporosis where the bone turnover rate is high, there is mobilization of various minerals including lead (Pb) from bone to blood. This study aimed to determine if there were any differences in serum Pb levels among elderly osteopaenic patients, elderly osteoporotic persons and healthy controls. Methods: Fifty-four elderly persons (26 men and 28 women) from the Amirkola Health and Ageing Project, Iran, were included in this study. The diagnosis of osteopaenia and osteoporosis was based on spine and femur bone mineral density (BMD) measurements. After blood sampling, serum Pb levels were analysed by the method of atomic absorption spectrophotometry. Results: According to the BMD measurements, 19 persons had normal BMD, while 16 had osteopaenia and 19 suffered from osteoporosis. The differences in body mass index in these three groups were statistically significant (p < 0.001). The patients with osteoporosis had the highest levels of alkaline phosphatase and the highest rate of bone turnover. The mean ± standard deviation of the serum Pb levels in these groups were 236.8 ± 98.0, 270.0 ± 81.5 and 258.3 ± 57.5 μg/L, respectively, and the differences were not statistically significant (p = 0.467). Conclusion: No statistically significant differences in serum Pb levels were observed in healthy controls compared with osteopaenic persons and osteoporotic persons. This suggests that mobilization of Pb from bone to blood in this population of elderly osteopaenic patients and elderly osteoporotic patients was similar to that in the healthy controls.
RESUMEN Objetivo: Se postula que en algunas condiciones como el embarazo y la osteoporosis donde el índice de recambio óseo es alto, hay movilización de varios minerales - incluyendo plomo (Pb) - de los huesos a la sangre. Este estudio tuvo como objetivo determinar si hubo diferencias en los niveles de plomo sérico entre los pacientes osteopénicos mayores de edad, los pacientes osteoporóticos mayores de edad, y los controles sanos. Métodos: Se incluyeron en este estudio 54 personas de edad avanzada (26 hombres y 28 mujeres) del Proyecto Amirkola de Salud y Envejecimiento, Irán. La diagnosis de la osteopenia y la osteoporosis se basó en mediciones de la densidad mineral ósea (DMO) de la espina dorsal y del fémur (DMO). Después del muestreo de sangre, los niveles séricos de Pb fueron analizados por el método de espectrofotometría de absorción atómica. Resultados: Según las mediciones de la DMO, 19 personas tenían DMO normal, mientras que 16 tenían osteopenia y 19 padecían osteoporosis. Las diferencias en el índice de masa corporal en estos tres grupos fueron estadísticamente significativas (p < 0.001). Los pacientes con osteoporosis tenían los niveles más altos de fosfatasa alcalina y el índice más alto de recambio óseo. La media ± desviación estándar de los niveles séricos de Pb en estos grupos fue de 236.8 ± 98.0, 270.0 ± 81.5 y 258.3 ± 57.5 μg/L, respectivamente, y las diferencias no fueron estadísticamente significativas (p = 0.467). Conclusión: No se observaron diferencias estadísticamente significativas en los niveles séricos de Pb en los controles sanos en comparación con las personas osteopénicas y las osteoporóticas. Esto sugiere que la movilización de Pb del hueso a la sangre en esta población de pacientes osteopénicos mayores de edad y pacientes osteoporóticos mayores de edad, era similar a la encontrada en los controles sanos.
Subject(s)
Humans , Male , Female , Middle Aged , Aged , Osteoporosis/blood , Bone Diseases, Metabolic/blood , Lead/blood , Spectrophotometry, Atomic , Biomarkers/blood , Bone DensityABSTRACT
BACKGROUND: Lead (Pb), mercury (Hg), and cadmium (Cd) are well-known environmental pollutants. They are unnecessary in the biological processes of humans. This study was performed to estimate the representative background exposure levels to the metals by measuring concentrations in whole blood of the Korean general population. METHODS: This population-based cross-sectional study included 4,000 subjects (1,886 males and 2,114 females) 0–83 years of age in 2010 and 2011. Adult subjects (≥ 19 years of age) were collected by sex- and age-stratified probability method, and preschool- and school-aged subjects were recruited by a cluster sampling method. Written consent was provided prior to blood sampling. Pb and Cd blood concentrations were determined by a flameless atomic absorption spectrophotometry, and blood Hg was analyzed by a direct Hg analyzer. RESULTS: The geometric mean, median and 95th percentile of blood Pb was 1.82 µg/dL, 1.83 µg/dL, and 3.78 µg/dL, respectively. The respective values were 2.92 µg/L, 2.87 µg/L, 9.12 µg/L for Hg, and 0.56 µg/L, 0.59 µg/L, 2.20 µg/L for Cd. Blood Pb and Hg were higher in males than in females, but no sex difference was observed, respectively, in subjects 0–4 years of age for Pb and in subjects less than 20 years for Hg. However, blood Cd was higher in females than in males and no sex difference was observed in subjects < 30 years of age. CONCLUSION: This study provides representative data of human exposure to Pb, Hg, and Cd covering whole age groups of the general population in Korea.
Subject(s)
Adult , Female , Humans , Male , Biological Phenomena , Cadmium , Cross-Sectional Studies , Environmental Pollutants , Korea , Metals , Methods , Sex Characteristics , Spectrophotometry, AtomicABSTRACT
Glues medicines is a special kind of traditional Chinese medicine.As the market demand is large, the raw materials are in short supply and lacks proper quality evaluation technology, which causes inconsistent quality of products on the market. Its authentic identification and evaluation stay a problem to be solved. In this paper, the research progress of the methods and techniques of the evaluation of the identification and quality of glues medicines were reviewed. The researches of medicinal glue type identification and quality evaluation mainly concentrated in four aspects of medicinal materials of physical and chemical properties, trace elements, organic chemicals and biological genetic methods and techniques. The methods of physicochemical properties include thermal analysis, gel electrophoresis, isoelectric focusing electrophoresis, infrared spectroscopy, gel exclusion chromatography, and circular dichroism. The methods including atomic absorption spectrometry, X-ray fluorescence spectrometry, plasma emission spectrometry and visible spectrophotometry were used for the study of the trace elements of glues medicines. The organic chemical composition was studied by methods of composition of amino acids, content detection, odor detection, lipid soluble component, organic acid detection. Methods based on the characteristics of biogenetics include DNA, polypeptide and amino acid sequence difference analysis. Overall, because of relative components similarity of the glues medicines (such as amino acids, proteins and peptides), its authenticity and quality evaluation index is difficult to judge objectively, all sorts of identification evaluation methods have different characteristics, but also their limitations. It indicates that further study should focus on identification of evaluation index and various technology integrated application combining with the characteristics of the production process.
Subject(s)
Adhesives , Drugs, Chinese Herbal , Medicine, Chinese Traditional , Quality Control , Spectrophotometry, Atomic , Trace ElementsABSTRACT
O objetivo foi avaliar o nível de exposição ao chumbo e mercúrio em população do Pantanal Mato-grossense, Brasil. Chumbo no sangue (Pb-S) (n = 119) e mercúrio na urina (Hg-U) (n = 109) de moradores da região foram determinados por espectrometria de absorção atômica. A comparação de médias e correlação entre as variáveis utilizaram o teste ANOVA e a regressão linear, respectivamente, com 95% de confiança. Pb-S médio foi 2,82 ± 1,53µg dL-1. A comparação de Pb-S estratificado por local de coleta (p ≤ 0,01), atividade laboral (p ≤ 0,01) e consumo de leite produzido na região (p ≤ 0,05) mostrou diferenças estatisticamente significativas. Também houve associações positivas entre Pb-S e local de coleta (p ≤ 0,01), profissão dos participantes (p ≤ 0,05), consumo de leite da região (p ≤ 0,01) e origem da água de consumo (p ≤ 0,01). A média de Hg-U foi 1,41 ± 0,98µg L-1. Os teores mostraram diferenças significativas apenas quanto à profissão dos participantes (p ≤ 0,01), e associações positivas surgiram entre Hg-U e atividade profissional (p ≤ 0,01) assim como índice de massa corporal dos sujeitos de estudo (p ≤ 0,01). As amostras apresentaram baixos níveis de chumbo e mercúrio, semelhantes àqueles encontrados em populações também expostas ambientalmente. Apesar dessas baixas concentrações, o conhecimento atual sobre toxicidade desses metais mostra que efeitos à saúde já podem ser sentidos em níveis antes considerados seguros, o que caracteriza o perigo.
El objetivo fue evaluar el nivel de exposición al plomo y mercurio en una población del Pantanal Mato-grossense, Brasil. El plomo en sangre (Pb-S) (n = 119) y mercurio en la orina (Hg-U) (n = 109) de los habitantes de esa región se determinó por espectrometría de absorción atómica. La comparación de medias y la correlación entre las variables utilizaron el test ANOVA y la regresión lineal, respectivamente, con un 95% de confianza. Pb-S medio fue 2,82 ± 1,53µg dL-1. La comparación de Pb-S estratificado por lugar de recogida (p ≤ 0,01), actividad laboral (p ≤ 0,01) y consumo de leche que se produjo en la región (p ≤ 0,05) mostró diferencias estadísticamente significativas. También hubo asociaciones positivas entre Pb-S y el lugar de recogida (p ≤ 0,01), profesión de los participantes (p ≤ 0,05), consumo de leche de la región (p ≤ 0,01) y origen del agua de consumo (p ≤ 0,01). La media de Hg-U fue 1,41 ± 0,98µg L-1. Los porcentajes mostraron diferencias significativas sólo respecto a la profesión de los participantes (p ≤ 0,01), y las asociaciones positivas surgieron entre Hg-U y la actividad profesional (p ≤ 0,01), así como índice de masa corporal de los sujetos de estudio (p ≤ 0,01). Las muestras presentaron bajos niveles de plomo y mercurio, semejantes a aquellos encontrados en poblaciones también expuestas ambientalmente. A pesar de esas bajas concentraciones, el conocimiento actual sobre toxicidad de estos metales muestra que los efectos sobre la salud, ya pueden ser apreciados en niveles antes considerados seguros, lo que caracteriza el peligro.
The objective was to assess the level of exposure to lead and mercury in a population in the Pantanal region in Mato Grosso State, Brazil. Blood lead (PbB) (n = 119) and urinary mercury (HgU) (n = 109) in local residents were measured by atomic absorption spectrometry. Comparison of means and correlations between variables used analysis of variance (ANOVA) and linear regression, respectively, with 95% confidence intervals. Mean PbB was 2.82 ± 1.53µg dL-1. The comparison of PbB stratified by collection site (p ≤ 0.01), work activity (p ≤ 0.01), and consumption of locally produced cow's milk (p ≤ 0.05) showed statistically significant differences. There were also positive associations between PbB and collection site (p ≤ 0.01), participants' profession (p ≤ 0.05), local milk (p ≤ 0.01), and source of drinking water (p ≤ 0.01). Mean HgU was 1.41 ± 0.98µg L-1. The levels only showed significant differences for participants' profession (p ≤ 0.01), and positive associations emerged between HgU and work activity (p ≤ 0.01) and body mass index (p ≤ 0.01). The samples showed low lead and mercury levels, similar to those found in other environmentally exposed populations. Despite these low concentrations, current knowledge on the toxicity of these metals shows that health effects can already be felt at levels that were previously considered safe, thus characterizing a health hazard.
Subject(s)
Humans , Male , Female , Adolescent , Adult , Middle Aged , Young Adult , Occupational Exposure/analysis , Environmental Exposure/analysis , Environmental Pollutants/analysis , Lead/blood , Mercury/urine , Spectrophotometry, Atomic , Brazil , Biomarkers/analysis , Cross-Sectional StudiesABSTRACT
Abstract Objectives To determine the concentration of calcium, iron, manganese and zinc ions after the application of chelator to Enterococcus faecalis biofilms. Material and Methods Fifty bovine maxillary central incisors were prepared and inoculated with E. faecalis for 60 days. The following were used as irrigation solutions: 17% EDTA (pH 3, 7 and 10), 2.5% sodium hypochlorite (NaOCl) combined with 17% EDTA (pH 3, 7 and 10), distilled water (pH 3, 7 and 10), and 2.5% NaOCl. Each solution was kept in the root canal for five minutes. Fifteen uncontaminated root canals were irrigated with 17% EDTA (pH 3, 7 and 10). Six teeth were used as bacterial control. The number of calcium, iron, manganese and zinc ions was determined using flame atomic absorption spectrometry. Mean ± standard deviation (SD) values were used for descriptive statistics. Results Calcium chelation using 17% EDTA at pH 7 was higher than at pH 3 and 10, regardless of whether bacterial biofilm was present. The highest concentration of iron occurred at pH 3 in the presence of bacterial biofilm. The highest concentration of manganese found was 2.5% NaOCl and 17% EDTA at pH 7 in the presence of bacterial biofilm. Zinc levels were not detectable. Conclusions The pH of chelating agents affected the removal of calcium, iron, and manganese ions. The concentration of iron ions in root canals with bacterial biofilm was higher after the use of 17% EDTA at pH 3 than after the use of the other solutions at all pH levels.
Subject(s)
Animals , Cattle , Chelating Agents/pharmacology , Enterococcus faecalis/drug effects , Biofilms/drug effects , Dental Pulp Cavity/drug effects , Dental Pulp Cavity/microbiology , Root Canal Irrigants/pharmacology , Root Canal Irrigants/chemistry , Sodium Hypochlorite/pharmacology , Sodium Hypochlorite/chemistry , Spectrophotometry, Atomic , Materials Testing , Water/chemistry , Chelating Agents/chemistry , Calcium/analysis , Edetic Acid/pharmacology , Edetic Acid/chemistry , Enterococcus faecalis/chemistry , Dental Pulp Cavity/chemistry , Hydrogen-Ion Concentration , Ions , Iron/analysis , Manganese/analysisABSTRACT
ABSTRACT Objective: Selenium (Se) supplementation has been used to help prevent the progression of Graves' ophthalmopathy (GO) and autoimmune thyroid diseases (AITD) patients. We investigated Se serum and selenoprotein P (SePP) levels in Graves' disease (GD) with and without GO, Hashimoto's thyroiditis (HT) patients and in 27 control individuals (C). Subjects and methods: We studied 54 female and 19 male patients: 19 with GD without GO, 21 GD with GO, 14 with HT and 19 with HT+LT4. Se values were measured using graphite furnace atomic absorption spectrophotometry. Serum SePP levels were measured by ELISA. Results: Median Se levels were similar among all groups; GD patients: 54.2 (46.5-61.1 μg/L), GO: 53.6 (43.5-60.0 μg/L), HT: 51.9 (44.6-58.5 μg/L), HT+LT4 54.4 (44-63.4) and C group patients: 56.0 (52.4-61.5 μg/L); P = 0.48. However, serum SePP was lower in GO patients: 0.30 (0.15-1.05 μg/mL) and in HT patients: 0.35 (0.2-1.17 μg/mL) compared to C group patients: 1.00 (0.564.21 μg/mL) as well as to GD patients: 1.19 (0.62-2.5 μg/mL) and HT+LT4 patients: 0.7 (0,25-1.95); P = 0.002. Linear regression analysis showed a significant relationship between SePP and TPOAb values (r = 0.445, R2 = 0.293; P < 0.0001). Multiple regression analysis found no independent variables related to Se or SePP. Conclusion: A serum Se concentration was lower than in some other countries, but not significantly among AITD patients. The low serum SePP levels in GO and HT patients seems to express inflammatory reactions with a subsequent increase in Se-dependent protein consumption remains unclear.
Subject(s)
Humans , Male , Female , Adult , Middle Aged , Aged , Selenium/blood , Graves Disease/blood , Hashimoto Disease/blood , Selenoprotein P/blood , Spectrophotometry, Atomic , Biomarkers/blood , Case-Control Studies , Cross-Sectional Studies , Disease Progression , Graves Ophthalmopathy/bloodABSTRACT
Abstract: Objective: This study determined the main dietary sources of urinary molybdenum (Mo) concentrations in a sample of 124 pregnant women in Mexico. Materials and methods: Dietary data was collected during pregnancy, through a semi-qualitative food frequency questionnaire, with information of 84 foods. Urine Mo levels were determined by atomic absorption spectrometry, for at least two trimesters of pregnancy. The associations with Mo levels were estimated by generalized mixed effect regression models. Results: Between 5.8 to 12.7% of the samples were above the 95th percentile of urinary Mo distribution reported by National Health and Nutrition Examination Survey (NHANES) 2009-2010 for women (151 μg/L and 148 μg/g creatinine). After bootstrap resampling was conducted, women with high-consumption of hot peppers (β=1.34μg/g; 95% CI: 1.00-1.80; p= 0.05) had marginally higher urinary Mo concentration levels, creatinine adjusted, compared to women with low-consumption. Conclusion. Hot chili pepper consumption may contribute to body burden Mo levels in this population.
Resumen: Objetivo: Determinar las fuentes dietéticas de molibdeno (Mo) urinario en 124 mujeres embarazadas residentes en el estado de Morelos, México. Material y métodos: Mediante un cuestionario de frecuencia de consumo de 84 alimentos, se obtuvo información dietética durante el embarazo. Las concentraciones urinarias de Mo se determinaron por espectrometría de absorción atómica, en al menos dos trimestres del embarazo. La asociación se estimó mediante modelos de efectos mixtos generalizados. Resultados: Entre 5.8 y 12.7% de las muestras superaron el P95 (151 µg/L y 148 µg/g creatinina) de la distribución de Mo urinario reportado para mujeres por la Encuesta Nacional de Nutrición y Salud de Estados Unidos (NHANES) 2009-2010. El mayor consumo de chile (β=1.34μg/g; IC95%: 1.00-1.80; p=0.05) se asoció con concentraciones marginalmente mayores de Mo. Conclusión: Probablemente debido a los fertilizantes o el sistema de riego utilizado en su cultivo, el consumo de chile es una posible fuente de exposición a Mo, en esta población.
Subject(s)
Humans , Female , Pregnancy , Adult , Young Adult , Diet , Molybdenum/urine , Pregnancy Trimesters/urine , Prenatal Exposure Delayed Effects , Socioeconomic Factors , Spectrophotometry, Atomic , Capsicum/chemistry , Pilot Projects , Feeding Behavior , Mexico , Molybdenum/toxicity , Molybdenum/pharmacokineticsABSTRACT
Abstract Objectives This study analyzed the capacity of Candida spp. from dental biofilm of HIV infected (HIV+) children to demineralize primary molar enamel in vitro by Transversal Microhardness (TMH), Polarized Light Microscopy (PLM) and the quantity of calcium ions (Ca2+) released from the enamel. Material and Methods Candida spp. samples were isolated from the supragingival biofilm of HIV+ children. A hundred and forty (140) enamel blocks were randomly assigned to six groups: biofilm formed by C. albicans (Group 1); mixed biofilm formed by C. albicans and C. tropicalis (Group 2); mixed biofilm formed by C. albicans and C. parapsilosis (Group 3); mixed biofilm formed by C. albicans, C. parapsilosis and C. glabrata (Group 4); biofilm formed by C. albicans ATCC (Group 5) and medium without Candida (Group 6). Enamel blocks from each group were removed on days 3, 5, 8 and 15 after biofilm formation to evaluate the TMH and images of enamel were analyzed by PLM. The quantity of Ca2+ released, from Groups 1 and 6, was determined using an Atomic Absorption Spectrophotometer. The SPSS program was used for statistical analysis and the significance level was 5%. Results TMH showed a gradual reduction in enamel hardness (p<0.05) from the 1st to 15th day, but mainly five days after biofilm formation in all groups. The PLM showed superficial lesions indicating an increase in porosity. C. albicans caused the release of Ca2+ into suspension during biofilm formation. Conclusion Candida species from dental biofilm of HIV+ children can cause demineralization of primary enamel in vitro.