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1.
Int. braz. j. urol ; 47(1): 112-119, Jan.-Feb. 2021. tab, graf
Article in English | LILACS | ID: biblio-1134303

ABSTRACT

ABSTRACT Purpose: Understanding the effects of high oxidation reduction potential (ORP) levels on sperm parameters will help to identify patients with unexplained and male factor infertility who may have seminal oxidative stress and determine if ORP testing is needed. This study aimed to evaluate the association between seminal ORP and conventional sperm parameters. Materials and Methods: A total of 58 patients who provided a semen sample for simultaneous evaluation of sperm parameters and ORP between January and September 2019 were enrolled in this retrospective study. To identify normal and high ORP levels, a static ORP (sORP) cut-off value of 1.36mV/106sperm/mL was used. Sperm parameters were compared between infertile men with normal sORP (control group, n=23) and high sORP values (study group, n=35). Results: Men with sORP values >1.36mV/106sperm/mL had significantly lower total sperm count (TSC) (p <0.001), sperm concentration (p <0.001) and total motile sperm count (TMSC) (p <0.001). In addition, progressive motility (p=0.04) and fast forward progressive motility (p <0.001) were significantly lower in the study group. A negative correlation was found between sORP and TSC (r=-0.820, p <0.001), sperm concentration (r=-0.822, p <0.001), TMSC (r=-0.808, p <0.001) and progressive motility (r=-0.378, p=0.004). Non-progressive motility positively correlated with sORP (r=0.344, p=0.010). Conclusions: This study has shown that TSC, sperm concentration, progressive motility and TMSC are associated with seminal oxidative stress, indicated by a sORP cut-off of 1.36mV/106sperm/mL. Presence of oligozoospermia, reduced progressive motilty or low TMSC in sperm analysis should raise the suspicion of oxidative stress and warrants seminal ROS testing.


Subject(s)
Humans , Male , Sperm Motility , Infertility, Male , Oxidation-Reduction , Semen , Sperm Count , Spermatozoa , Retrospective Studies
2.
National Journal of Andrology ; (12): 917-926, 2021.
Article in Chinese | WPRIM | ID: wpr-922176

ABSTRACT

Objective@#To evaluate the effects of transcutaneous electrical acupoint stimulation (TEAS) on the pregnancy outcome and sperm parameters in patients with idiopathic oligoasthenospermia.@*METHODS@#We searched PubMed, EMBASE, Cochrane Library, CNKI, VIP and Wanfang from inception till January 2020 for randomized controlled trials (RCT) with the keywords male infertility, oligozoospermia, asthenozoospermia, acupuncture, transcutaneous electrical acupoint stimulation, etc. Using the Cochrane risk bias tool, we evaluated the quality of the identified RCTs, and analyzed the primary outcomes, including pregnancy and live birth, and secondary outcomes, such as sperm concentration, motility and morphology.@*RESULTS@#Four RCTs with 321 subjects were included, of which none reported live birth and only one reported a pregnancy rate of 15% after treatment of 2 Hz TEAS. Neither 2 Hz (WMD: -3.01, 95% CI: -22.28 to 16.26) nor 100 Hz TEAS (WMD: -0.02, 95% CI: -5.29 to 5.56) had any significant effect on sperm concentration, while 100 Hz TEAS markedly improved the percentage of grade a sperm (WMD: 6.83, 95% CI: 2.10 to 11.57) compared with 2Hz TEAS (WMD: 2.31, 95% CI: 1.01 to 3.61). In comparison with the blank control, neither 2 Hz (WMD: 4.07, 95% CI: -5.15 to 13.29) nor 100 Hz TEAS (WMD: 6.59, 95% CI: -5.36 to 18.55) significantly affected the percentage of grade a + b sperm or total sperm motility.@*CONCLUSIONS@#The effect of TEAS on the pregnancy outcome is not yet clear. 100 Hz TEAS significantly improved the percentage of grade a sperm in idiopathic oligoasthenospermia patients, which, however, is to be further verified with more high-quality clinical studies.


Subject(s)
Acupuncture Points , Female , Humans , Pregnancy , Pregnancy Rate , Sperm Count , Sperm Motility
3.
Asian Journal of Andrology ; (6): 479-483, 2021.
Article in English | WPRIM | ID: wpr-888459

ABSTRACT

The novel coronavirus disease (COVID-19) pandemic is emerging as a global health threat and shows a higher risk for men than women. Thus far, the studies on andrological consequences of COVID-19 are limited. To ascertain the consequences of COVID-19 on sperm parameters after recovery, we recruited 41 reproductive-aged male patients who had recovered from COVID-19, and analyzed their semen parameters and serum sex hormones at a median time of 56 days after hospital discharge. For longitudinal analysis, a second sampling was obtained from 22 of the 41 patients after a median time interval of 29 days from first sampling. Compared with controls who had not suffered from COVID-19, the total sperm count, sperm concentration, and percentages of motile and progressively motile spermatozoa in the patients were significantly lower at first sampling, while sperm vitality and morphology were not affected. The total sperm count, sperm concentration, and number of motile spermatozoa per ejaculate were significantly increased and the percentage of morphologically abnormal sperm was reduced at the second sampling compared with those at first in the 22 patients examined. Though there were higher prolactin and lower progesterone levels in patients at first sampling than those in controls, no significant alterations were detected for any sex hormones examined over time following COVID-19 recovery in the 22 patients. Although it should be interpreted carefully, these findings indicate an adverse but potentially reversible consequence of COVID-19 on sperm quality.


Subject(s)
Adult , Asthenozoospermia/virology , COVID-19/physiopathology , China , Gonadal Steroid Hormones/blood , Humans , Male , Progesterone/blood , Prolactin/blood , SARS-CoV-2 , Semen/physiology , Semen Analysis , Sperm Count , Sperm Motility , Spermatozoa/physiology , Time Factors
4.
Article in Chinese | WPRIM | ID: wpr-879553

ABSTRACT

DNA methylation as an important aspect of epigenetics plays an important role in spermatogenesis and embryonic development. In recent years, researchers have found that male infertility, in particular abnormal semen quality, is related to abnormal DNA methylation. To further delineate the pathogenesis of male infertility and inspire new ideas for the treatment of male infertility, a comprehensive review over the correlation between abnormal methylation of imprinted genes, repetitive DNA elements and non-imprinted genes, semen quality (including sperm count, morphology, and vitality) and male infertility is provided.


Subject(s)
DNA Methylation , Humans , Infertility, Male/genetics , Male , Semen Analysis , Sperm Count , Spermatogenesis , Spermatozoa/pathology
5.
Braz. J. Pharm. Sci. (Online) ; 57: e19144, 2021. tab, graf
Article in English | LILACS | ID: biblio-1350234

ABSTRACT

Vildagliptin is an oral hypoglycemic agent used in the management of diabetes. Some oral antidiabetic drugs have been implicated in reproductive toxicity.The objective of this study was to investigate the effects of daily administration of vildagliptin at different dosages (0.35 mg/kg B.W., 0.70 mg/kg B.W. and 1.40 mg/kg B.W.) to male Wistar rats for 8 weeks. Sperm parameters, serum concentrations of testosterone, follicle stimulating hormone and luteinizing hormone and the histology of the testis of the rats were assessed. Another set of rats were also treated for 8 weeks and allowed to recover and the same parameters were assessed in them. Fertility study was conducted by determining their litter size. The results showed that vildagliptin administration significantly reduced sperm count and motility of the treated rats. It also significantly increased the number of abnormal sperms. Serum level of testosterone was significantly decreased while luteinizing hormone and follicle stimulating hormone levels showed no significant change. The histoarchitecture of the testis of the treated rats appeared visibly normal. The litter size was also significantly reduced. Most of the changes observed were dose dependent. However, these parameters were restored towards normal in the recovery group. Our results suggest that vildagliptin adversely affected sperm parameters, affected litter size and disrupted the pituitary - gonadal axis. These changes were however reversed upon cessation of drug administration.


Subject(s)
Animals , Male , Rats , Sperm Count/classification , Testosterone/agonists , Vildagliptin/administration & dosage , Testis/abnormalities , Fertility/drug effects , Vildagliptin/adverse effects
6.
National Journal of Andrology ; (12): 922-925, 2020.
Article in Chinese | WPRIM | ID: wpr-880293

ABSTRACT

Objective@#To explore the distribution of Traditional Chinese Medicine (TCM) syndrome types and their relationship with semen parameters in infertility male patients with varicocele (VC).@*METHODS@#Using Questionnaire on Clinical Symptoms of Varicocele-Caused Male Infertility, we made an investigation among 147 infertility male patients with VC, determined the types of their TCM syndromes, obtained their semen parameters, and analyzed the distribution of the TCM syndrome types and their correlation with semen parameters.@*RESULTS@#Of the TCM syndrome types identified, kidney deficiency and stagnated heat constituted the largest proportion (34.7%), and the mixed type accounted for a significantly higher percentage than the simple type (P < 0.05). The patients with kidney deficiency and stagnated heat, compared with those with other syndrome types, had a dramatically lower sperm concentration ([21.62 ± 9.25] vs [28.88 ± 12.92] ×10⁶/ml, P < 0.01), but a higher percentage of morphologically abnormal sperm ([98.33 ± 0.15]% vs [96.27 ± 0.18]%, P < 0.05) and DNA fragmentation index ([19.72 ± 3.17]% vs [10.96 ± 3.82]%, P < 0.01). No statistically significant differences were observed in the percentage of progressively motile sperm among different TCM syndrome types.@*CONCLUSIONS@#Kidney deficiency and stagnated heat is a main TCM syndrome type in infertility male patients with varicocele and correlated with sperm concentration, the percentage of morphologically abnormal sperm and DNA fragmentation index.


Subject(s)
DNA Fragmentation , Humans , Infertility, Male/diagnosis , Male , Medicine, Chinese Traditional , Semen , Semen Analysis , Sperm Count , Sperm Motility , Spermatozoa , Syndrome , Varicocele/diagnosis
7.
National Journal of Andrology ; (12): 900-905, 2020.
Article in Chinese | WPRIM | ID: wpr-880289

ABSTRACT

Objective@#To analyze the relationship of Mycoplasma genitalium (MG) infection with routine semen parameters and sperm DNA integrity in male infertility patients.@*METHODS@#Totally, 114 semen samples, 34 MG-positive and 80 MG-negative, were collected from male infertility patients and subjected to routine semen analysis with the computer-assisted sperm analysis system, Papanicolaou staining for observation of sperm morphology, and sperm chromatin diffusion (SCD) test for detection of sperm DNA integrity. Semen parameters and DNA integrity were compared between the MG-positive and MG-negative groups with SPSS 21.0 statistical software and the relationship between the semen parameters and DNA integrity analyzed by Pearson correlation analysis.@*RESULTS@#The MG-positive samples, compared with the MG-negative ones, showed significantly decreased semen volume ([2.87 ± 0.37] vs [3.86 ± 0.43] ml, P 0.05).@*CONCLUSIONS@#MG infection may be an important factor affecting sperm quality in male infertility patients. Active prevention and treatment of MG infection can help prevent male infertility.


Subject(s)
DNA Fragmentation , Humans , Infertility, Male/microbiology , Male , Mycoplasma Infections/complications , Mycoplasma genitalium , Semen , Semen Analysis , Sperm Count , Sperm Motility , Spermatozoa
8.
Rev. bras. anal. clin ; 51(2): 120-126, 20191011. tab, graf
Article in Portuguese | LILACS | ID: biblio-1024900

ABSTRACT

Objetivo: Identificar os parâmetros com maior incidência de erros no programa de controle de qualidade externo em espermograma e descrever o panorama da participação dos laboratórios brasileiros em ensaios de proficiência para espermograma. Métodos: Trata-se de um estudo descritivo, retrospectivo de análise de dados com abordagem quantitativa que descreve e avalia o desempenho de laboratórios de análises clínicas participantes do Programa Nacional de Controle de Qualidade (PNCQ) e Controllab entre os anos de 2010 ­ 2017. Resultados: Os resultados obtidos revelaram que até o momento existe um total de 273 participantes de controle externo da qualidade na especialidade de espermograma e, quando comparado com o total de laboratórios de análises clínicas no Brasil (20.800), esse percentual é de 1,3%. Em ambos os programas, o maior percentual de erros foi na análise da concentração espermática com 12,6% (PNCQ) e 20,7% Controllab) e esses dados podem inferir as deficiências de padronização na contagem espermática. Conclusão: Esses resultados apontam para um déficit de participação, que, apesar de não poder ser estatisticamente comprovado, alerta para a nula participação de alguns estados. Os ensaios de proficiência associados ao treinamento constante do pessoal do laboratório são importantes medidas em atenção à qualidade da análise seminal.


Objective: To identify the parameters with higher incidence of errors in the program of external quality control in spermogram and to describe the panorama of the participation of the Brazilian laboratories in tests of proficiency for spermogram. Methods: This is a descriptive, retrospective study of data analysis with a quantitative approach that describes and evaluates the performance of clinical analysis laboratories participating in the National Quality Control Program (PNCQ) and Controllab between the years 2010 - 2017. Results: The results show that up to now there are 273 participants of external quality control in the specialty of sperm


Subject(s)
Humans , Male , Quality Control , Sperm Count , Semen Analysis , Quality Improvement , Laboratory Proficiency Testing
9.
Int. braz. j. urol ; 45(5): 1043-1054, Sept.-Dec. 2019. tab, graf
Article in English | LILACS | ID: biblio-1040070

ABSTRACT

ABSTRACT Objective Anacyclus Pyrethrum (AP) and Tribulus Terrestris (TT) have been reported as male infertility treatment in several studies; however, in Iranian traditional medicine these two plants are prescribed simultaneously. In this study, we aimed to determine the effects of AP and TT extracts both separately and simultaneously on the male Wistar rat fertility parameters. Materials and Methods 32 male Wistar rats were divided into 4 groups: Control, TT, AP, and AT treated groups. Treatment continued for 25 days and rats were weighed daily. Their testes were dissected for histological studies. Sperm analysis including sperm count, viability and motility were performed. Serum was obtained to evaluate testosterone, LH and FSH levels. Histological studies were conducted to study Leydig, and Sertoli cells, spermatogonia and spermatid cell numbers, and to measure seminiferous diameter and epithelium thickness. Results Sperm count increased in all the treatment groups. Sperm viability and motility in AT and AP groups were elevated. TT and AT groups showed significantly increased testosterone level compared to control group (P=004, P=0.000, respectively) and TT, AP and AT treatment groups showed increased LH level (P=0.002, P=0.03 and P=0.000, respectively) compared to control, while only AT group showed increased FSH (p=0.006) compared to control. Histological studies showed significant increase of spermatogonia, Leydig and Sertoli cell numbers and epithelial thickness in AT group compared to other groups. All the treatment groups had higher number of Leydig, spermatogonia and spermatid cells. Conclusion TT and AP improved sexual parameters; however, their simultaneous administration had higher improving effects on studied parameters.


Subject(s)
Humans , Animals , Male , Chrysanthemum cinerariifolium/chemistry , Plant Extracts/therapeutic use , Tribulus/chemistry , Infertility, Male/drug therapy , Organ Size , Reference Values , Sperm Count , Sperm Motility/drug effects , Spermatozoa/drug effects , Testis/drug effects , Testosterone/blood , Body Weight , Luteinizing Hormone/blood , Plant Extracts/pharmacology , Reproducibility of Results , Treatment Outcome , Rats, Wistar , Fertility/drug effects , Follicle Stimulating Hormone/blood
10.
Int. braz. j. urol ; 45(4): 815-824, July-Aug. 2019. tab, graf
Article in English | LILACS | ID: biblio-1019894

ABSTRACT

ABSTRACT Introduction Chronic hyperglycemia is caused by diabetes mellitus-committed genital morphophysiology, and oxidative stress is one of the main factors involved in this process. Alpha lipoic acid (ALA) can prevent metabolic and morphological changes in diabetic individuals. Objectives In present study, we evaluated the effects of regular ALA consumption on the spermatogenesis and histoarchitecture in the male genital system of diabetic rats. Materials and Methods Thirty-two Wistar rats were divided into groups: Control (CG); Diabetic Control (DCG), receiving commercial diet: ALA Group (ALAG) and Diabetic ALA Group (DALAG), fed diets with added ALA (300 mg/Kg bw). The diabetic groups received a single injection of streptozotocin (60 mg/kg). After sixty days of the diet, the animals were euthanized, and semen, testis and epididymis samples were collected. A histomorphometric analysis was performed to determine the epithelial height, tubular and luminal diameter, tubular and luminal area of seminiferous tubules and each epididymal region. Sertoli cells were evidenced using the antivimenti antibody and were quantified. The results were statistically analyzed by the ANOVA test. Results At the end of the experiment, the DALAG glycemia was significantly lower than DCG. The histomorphometric parameters of the seminiferous and epididymal tubules did not show improvement in the DALAG. However, there was an improvement in the DALAG in terms of the concentration, motility and percentage of spermatic pathologies, as well as in the number of Sertoli cells (p<0.001). Conclusions The results demonstrated that supplementation with the ALA antioxidant retards testicular lesions and preserve the process of spermatogenesis in diabetes.


Subject(s)
Animals , Male , Spermatozoa/drug effects , Testis/drug effects , Thioctic Acid/pharmacology , Diabetes Mellitus, Experimental/pathology , Epididymis/drug effects , Antioxidants/pharmacology , Sertoli Cells , Sperm Count , Sperm Motility/drug effects , Spermatogenesis/drug effects , Spermatogenesis/physiology , Spermatozoa/physiology , Testis/physiopathology , Testis/pathology , Immunohistochemistry , Random Allocation , Reproducibility of Results , Rats, Wistar , Diabetes Mellitus, Experimental/physiopathology , Epididymis/pathology
11.
Rev. cuba. obstet. ginecol ; 45(1): 164-168, ene.-mar. 2019.
Article in Spanish | LILACS, CUMED | ID: biblio-1093632

ABSTRACT

Es aceptado que la infertilidad se evalúa cuando una pareja no logra embarazarse después de 12 meses de coitos regulares sin protección, problema que afecta a un alto porcentaje de parejas en el mundo. Generalmente, el diagnóstico y tratamiento de la infertilidad se enfoca solo desde el punto de vista femenino, por la obvia relación con el embarazo; sin embargo, con la evidencia existente el problema debe ser abordado como una alteración de pareja(AU)


It is accepted that infertility is evaluated when a couple does not become pregnant after 12 months of regular unprotected intercourse. This problem affects a large percentage of couples worldwide. Generally, diagnosis and treatment of infertility approaches only the female point of view, for the obvious connection with the pregnancy. However, this problem must be addressed with the actual evidence as an alteration in the couple(AU)


Subject(s)
Humans , Male , Female , Sperm Count/methods , Infertility, Male/diagnosis
12.
Arq. bras. med. vet. zootec. (Online) ; 71(1): 211-218, jan.-fev. 2019. tab, graf
Article in Portuguese | LILACS, VETINDEX | ID: biblio-989349

ABSTRACT

Objetivou-se estudar o efeito do ômega 3 e da vitamina B12 no espermograma, na histomorfometria dos órgãos reprodutivos e na temperaturas do corpo com termografia infravermelha em ratos Wistar. Utilizaram-se 16 ratos, em quatro grupos (n=4), que receberam injeções diárias por 30 dias, sendo: grupo controle - solução salina; grupo ômega 3 - óleo de peixe 1g/kg; grupo B12 - vitamina B12 3µg; e grupo ômega 3 + B12 - óleo de peixe 1g/kg e vitamina B12 3µg. Imagens termográficas de áreas do corpo foram obtidas. No 30º dia, os ratos foram sacrificados e realizaram-se as análises de morfologia espermática e histomorfometria. Os dados foram submetidos à análise de variância e ao teste de Tukey a 5%. A temperatura da superfície do escroto foi superior no grupo B12 (P<0,05). Não houve diferenças entre grupos (P>0,05) para temperaturas do globo ocular. Houve correlação entre temperatura da superfície do escroto e porcentagem de gota citoplasmática distal (P=0,678). A elevação da temperatura do escroto resulta no aumento da porcentagem de gotas citoplasmáticas distais. A temperatura do globo ocular não sofre influência significativa do ômega 3 e da vitamina B12. O ômega 3 reduz o epitélio seminífero, e a vitamina B12 minimiza esse efeito.(AU)


The objective of this study was to study the effect of Omega 3 and vitamin B12 on spermogram, histomorphometry of reproductive organs and body temperature with infrared thermography in Wistar rats. Sixteen rats were used in four groups (n= 4) who received daily injections for 30 days. Control Group - saline solution; Group Omega 3 - fish oil 1g/kg; Group B12 - vitamin B12 3μg and Group Omega 3 + B12 - fish oil 1g/kg and vitamin B12 3μg. Thermographic images of body were obtained. On the 30th day the rats were sacrificed and analyzes of sperm morphology and histomorphometry were performed. Data were submitted to analysis of variance and Tukey's test at 5%. The surface temperature of the scrotum was higher in group B12 (P< 0.05). There were no differences between groups (P> 0.05) for eyeball temperatures. There was a correlation between scrotal temperature and distal cytoplasmic droplet (P= 0.678). Elevation of scrotum temperature results in an increase in the percentage of distal cytoplasmic droplets. The temperature of the eyeball is not significantly influenced by Omega 3 and vitamin B12. Omega 3 reduces the seminiferous epithelium and vitamin B12 minimizes this effect.(AU)


Subject(s)
Animals , Rats , Vitamin B 12/adverse effects , Fatty Acids, Omega-3/adverse effects , Rats, Wistar/metabolism , Sperm Count/veterinary , Thermography/veterinary
13.
Braz. j. biol ; 79(1): 1-5, Jan.-Mar 2019. tab, graf
Article in English | LILACS | ID: biblio-984001

ABSTRACT

Abstract The objective of the present work was to assess the qualitative and quantitative characteristics of semen from the surubim do Iguaçu (Steindachneridion melanodermatum). Induced spermiation was achieved in eleven males with mean weight of 1.76 ± 0.48 kg and average age of two years and semen was collected by stripping. The average volume was 1.34 ± 0.73 mL. The duration of sperm motility was 154.4 ± 72.6 and 149.0 ± 77.5 seconds after activation with hatchery water and distilled water, respectively. The sperm concentration estimated by hemocytometer was 5.423 ± 2.155 x 1010 spermatozoa/mL. The results indicate that S. melanodermatum semen is easily obtained during the spawning season and the seminal characteristics are adequate insemination and subsequent in vitro fertilization.


Resumo O objetivo do presente trabalho foi avaliar características qualitativas e quantitativas do sêmen de surubim do Iguaçu (Steindachneridion melanodermatum). Para as análises, utilizaram-se onze machos com peso médio de 1,76±0,48 kg e idade média de dois anos, submetidos ao tratamento de hipofisação. O volume médio de sêmen coletado foi de 1,34 ± 0,73 mL. O tempo médio de motilidade dos espermatozoides após a coleta foi de 154,4 ± 72,6 e 149,0 ± 77,5 segundos para ativação com água do tanque e água destilada, respectivamente. Para a concentração espermática, a média foi de 5,423 ± 2,155 x1010 espermatozoides/mL. Os resultados indicam que o sêmen de S. melanodermatum é facilmente obtido no período reprodutivo analisado, e que as características seminais avaliadas são adequadas para reprodução induzida.


Subject(s)
Animals , Male , Semen/physiology , Sperm Count/veterinary , Catfishes/physiology
14.
Article in Chinese | WPRIM | ID: wpr-776255

ABSTRACT

OBJECTIVE@#To observe the clinical effect of grain-moxibustion combined with medicine therapy for asthenospermia and oligospermia.@*METHODS@#A tatal of 60 patients were randomized into an observation group (30 cases) and a control group (30 cases) according to 1︰1 ratio. In the control group, vitamin E capsules were taken orally one capsule each time, twice a day, and pills 6 g each time, three times a day for a total of 3 months. In the observation group, grain-moxibustion was applied at Guanyuan (CV 4),Shenshu (BL 23) and Zusanli (ST 36) based on the control group, once a week for 3 months, with a total of 12 times. The sperm concentration and sperm progressive motility were measured by automatic sperm quality analysis system in the two groups, and the clinical effects were compared. Sperm DNA fragmentation index (DFI) in the observation group was measured by sperm nucleus chromosome structure assay (SCSA).@*RESULTS@#①The sperm concentrations and sperm progressive motilities after 1-month, 2-month and 3-month of treatment were increased compared with those before treatment in the two groups (<0.01), and they were increased with time. In the two groups, 2-month and 1-month of treatment, 3-month and 2-month of treatment were compared, the sperm concentrations and sperm progressive motilities were significantly increased (<0.01). The sperm concentrations after 1-month, 2-month and 3-month of treatment in the observation group were higher than those in the control group (<0.01), the sperm progressive motility after 3-month of treatment in the observation group was higher than that in the control group (<0.05). ②After 3-month of treatment,the DFI in the observation group was significantly reduced compared with that before treatment (<0.01). ③The total effective rate in the observation group after 3-month of treatment was 86.7% (26/30), which was superior to 63.3% (19/30) in the control group (<0.05).@*CONCLUSION@#Grain-moxibustion combined with medicine therapy can improve sperm concentration and sperm progressive motility, enhance the integrity of sperm DNA.


Subject(s)
Humans , Male , Medicine, Chinese Traditional , Moxibustion , Oligospermia , Therapeutics , Sperm Count , Sperm Motility , Spermatozoa
15.
Article in English | WPRIM | ID: wpr-742356

ABSTRACT

PURPOSE: Sperm cryopreservation before cancer treatment is the most effective method to preserve the fertility of male patients. We present our 21 years experience with sperm cryopreservation for cancer patients, including an examination of semen quality, the current status of cryopreserved sperm, and the rate of sperm use for assisted reproductive technology (ART). MATERIALS AND METHODS: A total of 721 cancer patients at Fertility Center of CHA Gangnam Medical Center successfully performed sperm cryopreservation for fertility preservation from January 1996 to December 2016. Medical chart review was used to analyze patient age, marital status, cancer type, semen volume, sperm counts and motility, length of storage, and current banking status. RESULTS: The major cancers of the 721 patients were leukemia (28.4%), lymphoma (18.3%), testis cancer (10.0%). The mean age at cryopreservation was 27.0 years, and 111 patients (15.4%) performed sperm cryopreservation during or after cancer treatment. The mean sperm concentration was 66.7±66.3 ×106/mL and the mean sperm motility was 33.8%±16.3%. During median follow-up duration of 75 months (range, 1–226 months), 44 patients (6.1%) used their banked sperm at our fertility center for ART and 9 patients (1.2%) transferred their banked sperm to another center. The median duration from cryopreservation to use was 51 months (range, 1–158 months). CONCLUSIONS: Sperm cryopreservation before gonadotoxic treatment is the most reliable method to preserve the fertility of male cancer patients. Sperm cryopreservation should be offered as a standard of care for all men planning cancer therapy.


Subject(s)
Cryopreservation , Fertility Preservation , Fertility , Follow-Up Studies , Humans , Leukemia , Lymphoma , Male , Marital Status , Methods , Reproductive Techniques, Assisted , Semen , Semen Analysis , Semen Preservation , Sperm Count , Sperm Motility , Spermatozoa , Standard of Care , Testicular Neoplasms
16.
Article in English | WPRIM | ID: wpr-742352

ABSTRACT

PURPOSE: To investigate the effects of all-trans retinoic acid (ATRA) in cisplatin (CP)-induced testicular damage in rats. MATERIALS AND METHODS: Twenty-eight male Wistar rats were divided into four groups: Control, ATRA alone, ATRA+CP, and CP alone. Body weight, testicular weight, sperm count, sperm motility, percentage of abnormal sperm, total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI) in testicular tissue, and testicular histopathology were compared among groups. RESULTS: The sperm count and motility significantly decreased and the percentage of abnormal sperm significantly increased in the CP group compared to the control and ATRA groups. CP+ATRA administration significantly increased the sperm count and motility, but reduced the abnormal sperm count. CP administration significantly increased TOS and OSI compared to the control group and the other groups. Administering CP+ATRA significantly decreased TOS and the OSI in testicular tissue and reduced spermatogenesis, but increased the Johnsen score. CONCLUSIONS: The destructive effects of CP treatment on testicular tissue and spermatogenesis were reduced by administering ATRA.


Subject(s)
Animals , Body Weight , Cisplatin , Humans , Male , Oxidative Stress , Rats , Rats, Wistar , Sperm Count , Sperm Motility , Spermatogenesis , Spermatozoa , Testis , Tretinoin , Vitamin A
17.
Article in English | WPRIM | ID: wpr-763352

ABSTRACT

Obstructive azoospermia caused by acute epididymitis is usually permanent, and microsurgical vasoepididymostomy is the only reconstructive treatment option. There have been no reports of delayed recovery of sperm count after over 1 year in a patient with obstructive azoospermia related to history of acute epididymitis. We present a young male patient who had azoospermia and a history of acute epididymitis who experienced delayed recovery, with complete restoration of sperm production and the ability to conceive naturally.


Subject(s)
Azoospermia , Epididymitis , Humans , Infertility , Male , Sperm Count , Spermatozoa
18.
Braz. arch. biol. technol ; 62: e19180374, 2019. tab, graf
Article in English | LILACS | ID: biblio-1055387

ABSTRACT

Abstract Cases of some grave side effects of some allopathic medicines used in treatment of infertility has caused a global need for alternatives with minimal or no side effect, hence the demand to evaluate the fertility enhancing potential of omega-3 fatty acids (O3FA) in male rats. This study evaluated the fertility enhancing potential of omega-3 fatty acids (O3FA) in male rats. Seventy-two sexually mature male albino rats 11-13 weeks of age, weighing between 171 - 180 g were assigned into six groups (I - VI) fed graded doses of O3FA. Administration of O3FA lasted twenty-eight days at 48 hour intervals. At the end of the treatment, organosomatic index of testes, testicular and epididymis sperm cells counts and testicular histology were assessed following standard methods. The actual and relative testicular weights, testicular and epididymis sperm counts of all O3FA treated rats were significantly increased (p < 0.05) when compared with the control group rats. The photomicrographs of testes in O3FA treated rats showed normal spermatogonial cell layers and active spermatogenesis with appearance of spermatids in the lumen of some tubules. The findings of this study depicts that O3FA possesses the potency of enhancing various fertility indices in male rats with regards to absolute and relative testicular weights as well as sperm counts.


Subject(s)
Animals , Sperm Count/instrumentation , Testis/anatomy & histology , Fatty Acids, Omega-3/therapeutic use , Infertility, Male/drug therapy , Rats, Wistar
19.
Article in English | WPRIM | ID: wpr-763489

ABSTRACT

The objective of this study was to assess the association between dietary antioxidant intake and semen quality parameters in infertile men. In this cross-sectional study, dietary antioxidant intake was evaluated in 175 infertile Iranian men by a validated dish-based 106-item semi-quantitative food frequency questionnaire. Men were asked to abstain from ejaculation for at least 72 hours before sample collection. Semen parameters were assessed by a sperm counting chamber and Terminal deoxynucleotidyl transferase dUTP nick end labeling assay methods. Linear quantile regression was used to determine the associations between antioxidant nutrient intake and semen quality parameters (including total sperm count, sperm density, total motility, DNA damage and DNA fragmentation). Mean age of study participants was 32.19 ± 2.34 years. Compared with the lowest quartile, men in the highest quartile of dietary β-carotene and vitamin C intake had lower sperm DNA fragmentation index (Ptrend = 0.042 and Ptrend = 0.03, respectively). Also, dietary intake of beta-cryptoxanthin had a positive association with sperm density (Ptrend = 0.02), and dietary lutein was associated with total sperm count (P(trend) = 0.045). Dietary intake of other antioxidants did not significantly correlate with the indicators related to the quantity and quality of sperm (p > 0.05). These data suggest that dietary intake of some of the antioxidants is associated with semen related parameters.


Subject(s)
Antioxidants , Ascorbic Acid , Cross-Sectional Studies , Cryptoxanthins , DNA , DNA Damage , DNA Fragmentation , DNA Nucleotidylexotransferase , Ejaculation , Humans , Infertility , Lutein , Male , Oxidative Stress , Semen Analysis , Semen , Sperm Count , Spermatozoa
20.
Int. braz. j. urol ; 44(5): 972-980, Sept.-Oct. 2018. tab, graf
Article in English | LILACS | ID: biblio-975624

ABSTRACT

ABSTRACT Purpose: To evaluate the impact of overactive bladder disorder on patients diagnosed with retrograde ejaculation. Materials and Methods: Retrospective analysis of prospective collected database made. Questionnaires conducted in urology polyclinics in five different centers. Main Outcome Measure(s): International Index of Erectile Function - 5 (IIEF - 5), Overactive Bladder 8 - Question Awareness Tool (OAB - V8), urodynamics, semen analysis. The participants of the study were n = 120 patients. There was retrograde ejaculation (RE) in only n = 47 patients (non / minimal symptomatic patients), n = 73 patients had RE and overactive (OAB) complaints (symptomatic patients) and received anticholinergic treatment (trospium), n = 37 control group patients who only had OAB and received an anticholinergic. Results: While no difference was observed in overactive bladder examination and urodynamic values between the non / minimal symptomatic group and the symptomatic group (p > 0.05), sperm was detected and identified as fructose positive in post - ejaculation urine in the symptomatic group. Thus, it was possible to demonstrate the differences between symptomatic patients and non - symptomatic patients. Consequently, following three - month daily treatment with trospium 30 mg 2 x 1 in the control group and the symptomatic group, it was observed that an evident increase was observed in the sperm count and ejaculate volume in the symptomatic group and that no change was observed in the control group (p < 0.05). Conclusion: This clinical study is the first of its kind in terms of revealing the coexistence of RE with OAB upon performing urodynamics and showing that treatment is possible in selected patients.


Subject(s)
Humans , Male , Adult , Oligospermia/complications , Cholinergic Antagonists/therapeutic use , Ejaculation/physiology , Urinary Bladder, Overactive/complications , Oligospermia/physiopathology , Oligospermia/drug therapy , Sperm Count , Case-Control Studies , Retrospective Studies , Urinary Bladder, Overactive/physiopathology , Urinary Bladder, Overactive/drug therapy
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