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Rev. chil. obstet. ginecol. (En línea) ; 85(4): 312-323, ago. 2020. tab, graf
Article in Spanish | LILACS | ID: biblio-1138627


OBJETIVO: El objetivo de este trabajo fue establecer el efecto de la borra de café sobre la movilidad y los parámetros funcionales de los espermatozoides humanos in vitro. MATERIALES Y MÉTODOS: La borra de café, un subproducto obtenido en establecimientos especializados en la preparación de café soluble a base de grano, se diluyo en tampón fosfato salino y se mezcló en proporciones iguales con las muestras de semen de 16 voluntarios aparentemente sanos. A cada muestra se le determinó el efecto sobre la movilidad espermática en función del tiempo (30, 60, 90 y 120 minutos, n=16) y sobre los parámetros funcionales (n=6) por medio de citometría de flujo: potencial de membrana mitocondrial, producción de especies reactivas de oxígeno y lipoperoxidación de la membrana espermática. RESULTADOS: La incubación de los espermatozoides con la borra de café evidencio un cambio positivo en la movilidad espermática. Adicionalmente, la incubación con la borra de café incremento significativamente el potencial de membrana mitocondrial en los espermatozoides. CONCLUSIÓN: La borra de café, seguramente debido a los compuestos antioxidantes, afecta positivamente la movilidad espermática aumentando el potencial de membrana mitocondrial. Por lo tanto, esto es un paso inicial en la búsqueda de un suplemento de origen natural que aumente la calidad seminal.

OBJECTIVE: The objective of this work is to establish the effect of spent coffee grounds on the motility and functional parameters of human spermatozoa, in vitro. MATERIALS AND METHODS: Spent coffee grounds, a by-product obtained in specialized establishments in the preparation of soluble coffee based on grain, was diluted in saline phosphate buffer and mixed in equal proportions with semen samples from 16 apparently healthy volunteers. Each sample was determined the effect on sperm motility as a function of time (30, 60, 90 and 120 minutes, n=16) and on functional parameters (n=6) by means of flow cytometry: mitochondrial membrane potential, reactive oxygen species production and membrane lipoperoxidation. RESULTS: The incubation of the spermatozoa with the spent coffee grounds showed a positive change in sperm motility. Additionally, incubation with spent coffee grounds significantly increased the mitochondrial membrane potential in human sperm cells. CONCLUSION: Spent coffee grounds, probably due to antioxidant compounds, positively affects sperm motility by increasing mitochondrial membrane potential. Therefore, this is an initial step in the search for a supplement of natural origin that increases seminal quality.

Humans , Male , Adult , Young Adult , Semen/drug effects , Sperm Motility/drug effects , Spermatozoa/drug effects , Plant Extracts/pharmacology , Coffee/chemistry , Semen/physiology , Sperm Motility/physiology , Spermatozoa/physiology , Time Factors , In Vitro Techniques
Int. braz. j. urol ; 45(5): 1043-1054, Sept.-Dec. 2019. tab, graf
Article in English | LILACS | ID: biblio-1040070


ABSTRACT Objective Anacyclus Pyrethrum (AP) and Tribulus Terrestris (TT) have been reported as male infertility treatment in several studies; however, in Iranian traditional medicine these two plants are prescribed simultaneously. In this study, we aimed to determine the effects of AP and TT extracts both separately and simultaneously on the male Wistar rat fertility parameters. Materials and Methods 32 male Wistar rats were divided into 4 groups: Control, TT, AP, and AT treated groups. Treatment continued for 25 days and rats were weighed daily. Their testes were dissected for histological studies. Sperm analysis including sperm count, viability and motility were performed. Serum was obtained to evaluate testosterone, LH and FSH levels. Histological studies were conducted to study Leydig, and Sertoli cells, spermatogonia and spermatid cell numbers, and to measure seminiferous diameter and epithelium thickness. Results Sperm count increased in all the treatment groups. Sperm viability and motility in AT and AP groups were elevated. TT and AT groups showed significantly increased testosterone level compared to control group (P=004, P=0.000, respectively) and TT, AP and AT treatment groups showed increased LH level (P=0.002, P=0.03 and P=0.000, respectively) compared to control, while only AT group showed increased FSH (p=0.006) compared to control. Histological studies showed significant increase of spermatogonia, Leydig and Sertoli cell numbers and epithelial thickness in AT group compared to other groups. All the treatment groups had higher number of Leydig, spermatogonia and spermatid cells. Conclusion TT and AP improved sexual parameters; however, their simultaneous administration had higher improving effects on studied parameters.

Humans , Animals , Male , Chrysanthemum cinerariifolium/chemistry , Plant Extracts/therapeutic use , Tribulus/chemistry , Infertility, Male/drug therapy , Organ Size , Reference Values , Sperm Count , Sperm Motility/drug effects , Spermatozoa/drug effects , Testis/drug effects , Testosterone/blood , Body Weight , Luteinizing Hormone/blood , Plant Extracts/pharmacology , Reproducibility of Results , Treatment Outcome , Rats, Wistar , Fertility/drug effects , Follicle Stimulating Hormone/blood
Int. braz. j. urol ; 45(4): 815-824, July-Aug. 2019. tab, graf
Article in English | LILACS | ID: biblio-1019894


ABSTRACT Introduction Chronic hyperglycemia is caused by diabetes mellitus-committed genital morphophysiology, and oxidative stress is one of the main factors involved in this process. Alpha lipoic acid (ALA) can prevent metabolic and morphological changes in diabetic individuals. Objectives In present study, we evaluated the effects of regular ALA consumption on the spermatogenesis and histoarchitecture in the male genital system of diabetic rats. Materials and Methods Thirty-two Wistar rats were divided into groups: Control (CG); Diabetic Control (DCG), receiving commercial diet: ALA Group (ALAG) and Diabetic ALA Group (DALAG), fed diets with added ALA (300 mg/Kg bw). The diabetic groups received a single injection of streptozotocin (60 mg/kg). After sixty days of the diet, the animals were euthanized, and semen, testis and epididymis samples were collected. A histomorphometric analysis was performed to determine the epithelial height, tubular and luminal diameter, tubular and luminal area of seminiferous tubules and each epididymal region. Sertoli cells were evidenced using the antivimenti antibody and were quantified. The results were statistically analyzed by the ANOVA test. Results At the end of the experiment, the DALAG glycemia was significantly lower than DCG. The histomorphometric parameters of the seminiferous and epididymal tubules did not show improvement in the DALAG. However, there was an improvement in the DALAG in terms of the concentration, motility and percentage of spermatic pathologies, as well as in the number of Sertoli cells (p<0.001). Conclusions The results demonstrated that supplementation with the ALA antioxidant retards testicular lesions and preserve the process of spermatogenesis in diabetes.

Animals , Male , Spermatozoa/drug effects , Testis/drug effects , Thioctic Acid/pharmacology , Diabetes Mellitus, Experimental/pathology , Epididymis/drug effects , Antioxidants/pharmacology , Sertoli Cells , Sperm Count , Sperm Motility/drug effects , Spermatogenesis/drug effects , Spermatogenesis/physiology , Spermatozoa/physiology , Testis/physiopathology , Testis/pathology , Immunohistochemistry , Random Allocation , Reproducibility of Results , Rats, Wistar , Diabetes Mellitus, Experimental/physiopathology , Epididymis/pathology
Journal of Veterinary Research. 2017; 72 (1): 87-101
in Persian | IMEMR | ID: emr-187514


Background: Wheat sprout contains a high amount of antioxidants, vitamins [especially vitamin E], minerals and phytoestrogen compounds. Use of medicinal herbs in reducing heavy metal toxicities has increased worldwide. In recent years, negative effects of lead on the male reproductive system and sperm fertility parameters have been shown broadly

Objectives: This study investigated the effects of wheat sprout extract [WSE] and vitamin E on sperm parameters and testicular oxidative stress in rats exposed to lead acetate

Methods: Thirty-five rats were divided randomly into seven groups: Gl [control group] received 1 ml/kg/day of normal saline, G2 received 20 mg/kg/day of lead acetate, G3 and G4 received 100 mg/kg/day and 200 mg/kg/day of WSE respectively, G5 and G6 received 100 mg/kg/day and 200 mg/kg/day of WSE respectively with 20 mg/kg/day of lead acetate, and G7 received 100 mg/kg/day of vitamin E with 20 mg/kg/day of lead acetate. After 35 days, rats were sacrificed and blood, sperm, liver and testicle tissue samples were collected for histomorphological and histochemical studies

Results: Results showed that count, motility and viability of sperms increased following the administration of 200 mg/kg/day of WSE [p<0.01]. Histomorphological studies showed a significant increase in tubular differentiation index [TDI], Repopulation index [RI], number of Sertoli cells, and epithelium of seminiferous tubules in groups receiving 200 mg/kg/day of WSE [p<0.00l]

Conclusions: Results of the current study show that dose dependent WSE significantly prevents testicular toxicity and oxidative stress effects of lead acetate

Animals, Laboratory , Male , Oxidative Stress , Testis/drug effects , Spermatozoa/drug effects , Sperm Motility/drug effects , Sperm Count , Organometallic Compounds/adverse effects , Rats
Int. j. morphol ; 34(2): 796-803, June 2016. ilus
Article in English | LILACS | ID: lil-787071


Usage of medicinal plants for the treatment of diseases is a remarkable strategy considered in both clinical and experimental studies. This study was conducted to evaluate the effect of Tribulus terrestris hydroalcoholic extract administration on the serum level of glucose and reproductive parameters in diabetic male rats. In this experimental study, 30 adult male Wistar rats (200­250 g) were divided into 5 groups (n= 6). Diabetes was induced by a single intraperitoneal injection of 50 mg/kg streptozotocin. Experimental groups received daily Intraperitoneal injection of the extract (100, 250 and 500 mg/kg) since one week after inducing diabetes for two weeks. At the end of the experiment, the serum levels of testosterone and glucose were examined by ELISA and Glucodr devices respectively. Sperm parameters including morphology, count and motility were evaluated from the cauda epididymis. The diameters of seminiferous tubules were assessed based on 5 µm paraffin section of H&E stained section of testes. The data were evaluated by One Way ANOVA followed by post-hoc test using SPSS software. In T. terrestris extract groups, there were significant reductions in the levels of blood glucose while increased sperm motility, sperm count and seminiferous tubules diameter, percentage of sperms with normal morphology, level of testosterone hormone and final body weight compared with diabetic group (p <0.001). In conclusion, T. terrestris hydroalcoholic extract decreases serum level of glucose and diminishes the side effects of diabetes on male reproductive system showing by increasing in the serum level of testosterone, improving sperm parameters and returning the seminiferous tubules to normal diameter by a dose dependent manner.

El uso de plantas medicinales para el tratamiento de enfermedades es una importante estrategia considerada en estudios clínicos y experimentales. El objetivo fue evaluar el efecto de la administración de extracto de Tribulus terrestris hidroalcohólico en el nivel sérico de glucosa y los parámetros reproductivos en ratas macho diabéticas. En este estudio experimental, se dividieron 30 ratas Wistar (200­250 g) macho adultas, en 5 grupos (n= 6). Se indujo diabetes por una sola inyección intraperitoneal (i.p.) de 50 mg/kg de estreptozotocina. Los grupos experimentales recibieron inyección i.p diaria del extracto (100, 250 y 500 mg/kg) una semana después de la inducción de la diabetes durante dos semanas. Al final del experimento, los niveles séricos de testosterona y de glucosa se examinaron por ELISA y el dispositivos Glucodr, respectivamente. Además, fueron evaluados los parámetros de morfología, conteo y motilidad espermática. Los diámetros de los túbulos seminíferos de los testículos se evaluaron sobre la base de secciones de parafina de 5 mm teñidos con H&E. Los datos fueron evaluados por ANOVA de una vía seguido de prueba post-hoc utilizando el programa SPSS (versión 20). En los grupos tratados con extracto de T. terrestris hubo una reducción significativa en los niveles de glucosa en la sangre, mientras que se observó un aumento en el recuento y motilidad espermática, el diámetro de los túbulos seminíferos, el porcentaje de espermatozoides con morfología normal, el nivel de testosterona y el peso corporal final de las ratas en comparación con el grupo de ratas diabéticas (p <0,001). En conclusión, el extracto de T. terrestris hidroalcohólico disminuye los niveles séricos de glucosa y los efectos secundarios de la diabetes en el sistema reproductivo mediante el aumento de los niveles séricos de testosterona, mejorando los parámetros de los espermatozoides y la restitución a diámetro normal de los túbulos seminíferos dependiendo de la dosis.

Animals , Male , Rats , Blood Glucose/drug effects , Diabetes Mellitus, Experimental , Hydroalcoholic Solution , Plant Extracts/pharmacology , Reproduction/drug effects , Tribulus , Rats, Wistar , Sperm Count , Sperm Motility/drug effects , Testosterone/blood
Int. j. morphol ; 34(1): 102-109, Mar. 2016. ilus
Article in English | LILACS | ID: lil-780482


Testicular toxicity has been implicated in highly active anti-retroviral therapy (HAART) treatment. Hence there is need to identify an effective antioxidant product that can alleviate testicular necrosis due to HAART administration. Forty eight adult male Sprague-Dawley rats were used in this study. The animals were divided into eight (8) groups: A-H (n= 6). Group A animals received normal saline as the control; Group B was given Nevirapine (Nv); Group C was given Kolaviron (Kv); Group D was given vitamin C; Group E was given Nv and Kv; Group F was given Nv and Vitamin C; Group G was given Nv for 56 d and Kv for 28 d serving as a withdrawal group; Group H was given corn oil. Nv, Kv and Vit. C were given at 1.54, 200 and 250 (mg·kg)/bw respectively while all administrations were through oral gavage. The body weights were taken every other day. Thereafter, they were anaesthetized with halothane. The testes were excised, weighed, fixed in Bouin's fluid and stained with H&E while the epididymes removed for semen fluid analyses. The results showed a significant (P<0.05) decrease in sperm motility in group E (Nevirapine + kolaviron) when compared with group F (Nevirapine + Vitamin C) while Sperm count was not significantly different (P>0.05) across the groups. The testicular histoarchitectural studies revealed indistinct spermatogonia, necrotic interstititial endocrine cells in the altered interstitial space, fragmented spermatids, atrophy of mature spermatocytes, degenerated germ cells, obliterated seminiferous tubules lumen, undifferentiated spermatogonia and cellular debris in the somniferous tubules lumen of nevirapine administered group but normal across the other groups. In the testis, there were no significant reduction in SOD, Catalase and GPx activities but a significant decrease in GST activity (P<0.001) when group E was compared with group F. In conclusion, vitamin C presents a better remediation in nevirapine induced spermiotoxicity compared to kolaviron in Sprague-Dawley rats.

La toxicidad testicular ha sido implicada en la terapia antirretroviral altamente activa (TARAA). Por lo tanto existe la necesidad de identificar un producto antioxidante eficaz que pueda aliviar la necrosis testicular en la administración de la TARAA. Cuarenta y ocho ratas macho Sprague-Dawley adultas fueron utilizadas. Los animales se dividieron en ocho (8) grupos: AH (n= 6). Grupo A, animales recibieron solución salina normal como el control; Grupo B, recibió Nevirapina (Nv); Grupo C, recibió Kolaviron (Kv); Grupo D, recibió vitamina C; Grupo E, recibió Nv y Kv; Grupo F, recibió Nv y vitamina C; Grupo G, recibió Nv durante 56 d y Kv por 28 d como un grupo de retirada; Grupo H, recibió aceite de maíz. Nv, Kv y Vit. C se administraron en dosis de 1, 54, 200 y 250 (mg · kg) de peso corporal respectivamente; todas las administraciones fueron por sonda oral. Los pesos corporales se tomaron cada dos días. A partir de ese momento los animales fueron anestesiados con halotano. Los testículos fueron extirpados, pesados y fijados en solución de Bouin y teñidos con H&E, mientras que el epidídimo se retiró para analizar el semen. Los resultados mostraron un descenso (p<0,05) en la motilidad de los espermatozoides en el grupo E (Nevirapina + Kolaviron) en comparación con el grupo F (Nevirapina + vitamina C), mientras que el recuento espermático no mostró diferencias significativas (P>0,05) entre los grupos. El estudio de la histoarquitectura testicular reveló espermatogonias indiferenciadas, con células intersticiales necróticas en el espacio intersticial y espermátidas fragmentadas. Además, en el grupo que recibió Nevirapina mostró espermatocitos maduros atrofiados, degeneración de células germinales, lumen de los túbulos seminíferos obliterados, espermatogonias indiferenciadas y restos celulares en el lumen de los tubulos seminíferos. En el resto de los grupos los resultados fueron normales. En el testículo hubo una reducción significativa en las actividades de la superóxido dismutasa, catalasa y glutatión peroxidasa, pero una disminución significativa en la actividad glutatión S-transferasa (P <0,001) al comparar los grupo E y F.

Animals , Male , Rats , Antioxidants/pharmacology , Garcinia kola/chemistry , Nevirapine/toxicity , Plant Extracts/pharmacology , Superoxide Dismutase/antagonists & inhibitors , Testis/drug effects , Anti-HIV Agents/toxicity , Ascorbic Acid/pharmacology , Biflavonoids/pharmacology , Body Weight , Catalase/antagonists & inhibitors , Glutathione Peroxidase/antagonists & inhibitors , Rats, Sprague-Dawley , Seeds , Sperm Count , Sperm Motility/drug effects , Testis/enzymology , Testis/pathology
Int. braz. j. urol ; 41(4): 773-790, July-Aug. 2015. tab, graf
Article in English | LILACS | ID: lil-763046


ABSTRACTPurpose:This study aimed to investigate the protective effects of isolated and co-administration of vitamin E (VitE) and dexamethasone (DEX) on varicocele (VCL)-induced damages in testicular tissue.Materials and Methods:Wistar rats were divided into five groups (n=6), including; control-sham, non-treated VCL-induced, VitE-treated VCL-induced (VitE, 150 mg/kg, orally), DEX-administrated VCL-induced (DEX, 0.125 mg/kg, i.p.), VitE+DEX-received VCL-induced animals. The antioxidant status analyses, histopathological examinations, hormonal assay and tissue levels of alkaline phosphatase (ALP) were analyzed. The germinal epithelium RNA damage and Leydig cells steroidogenesis were analyzed. Moreover, the Hsp70-2 protein expression was examined based on immunohistochemical and western blot analyses. The sperm parameters, DNA integrity and chromatin condensation were investigated.Results:VitE and DEX in simultaneous form of administration significantly (P<0.05) down-regulated the tissue ALP level and attenuated the VCL-decreased GSH-px, SOD and TAC levels and remarkably (P<0.05) down-regulated the testicular malondialdehyde (MDA) and nitric oxide (NO) contents. The VCL-induced histopathological alterations significantly (P<0.05) improved in VitE and DEX-administrated animals. The VitE and DEX co-administration reduced the VCL-increased RNA damage and elevated the Leydig cells steroidogenic activity. The Hsp70-2 protein level completely (P<0.05) increased in VitE and DEX alone–and-simultaneous-administrated animals. Finally, the VitE and DEX could significantly (P<0.05) improve the VCL-decreased semen quality and improved the sperm DNA integrity and chromatin condensation.Conclusion:Our data suggest that Vit E by up-regulating the antioxidant status and DEX by reducing inflammation-dependent oxidative and nitrosative stresses could improve the VCL-reduced Hsp70-2 chaperone expression and ultimately protected the testicular endocrine activities and promoted the spermatogenesis process.

Animals , Male , Anti-Inflammatory Agents/administration & dosage , Antioxidants/administration & dosage , Dexamethasone/administration & dosage , /metabolism , Varicocele/drug therapy , Vitamin E/administration & dosage , Blotting, Western , Chromatin/physiology , Disease Models, Animal , DNA Damage , Drug Interactions , Glutathione Peroxidase/analysis , Immunohistochemistry , Malondialdehyde/analysis , Oxidative Stress/drug effects , Protective Agents , Rats, Wistar , Sperm Count , Sperm Motility/drug effects , Spermatozoa/drug effects , Superoxide Dismutase/analysis , Testis/drug effects , Testis/enzymology , Testis/pathology , Testosterone/blood , Varicocele/physiopathology
Braz. j. biol ; 75(3): 662-669, Aug. 2015. tab, ilus
Article in English | LILACS | ID: lil-761585


AbstractThis study aims developing and evaluate a protocol of semen cryopreservation of the lane snapper Lutjanus synagris. Firstly, sperm motility rate, motility time, density and spermatocrit were appraised to characterize the sperm quality of the lane snapper. The effect of three extenders with distinct ionic compositions and pH values combined with seven concentrations of cryoprotector dimethylsulfoxide (0; 2.5; 5.0; 7.5; 10.0; 12.5 e 15.0%), five cooling rates (110, 90, 60, 45 e 30°C –min), nine equilibration time (1; 2,5; 5; 10; 15; 20; 25; 30 e 60 minutes) e five dilutions ratio (1:1; 1:3; 1:6; 1:10 e 1:20) on the sperm motility rate and motility time were analyzed. Fertilization test was accomplished to evaluate the viability of the cryopreserved sperm. The higher sperm motility rate and motility time (P<0.05) was achieved by combining extender with pH 8.2 with 10% concentration of dimethylsulfoxide and cooling rate 60°C –min, 1 minute of equilibration time and 1:3 (v/v) dilution ratio. The use of cryopreserved sperm presented fertilization rates >60% validating the present protocol for lane snapper. The cryoconserved sperm of lane snapper is a viable alternative, being possible to maintain appropriate sperm viability.

ResumoEste estudo teve a finalidade de desenvolver e avaliar um protocolo de crioconservação do sêmen do ariocó Lutjanus synagris. Para caracterizar o sêmen foram avaliados a taxa de motilidade, a duração da motilidade, a concentração espermática e o espermatócrito. Em seis experimentos foram analisados os efeitos de três diluentes, com distintas composições iônicas e valores de pH distintos, combinados com sete concentrações de dimetilsulfóxido (0; 2,5; 5,0; 7,5; 10,0; 12,5 e 15,0%), cinco velocidades de congelamento (–110, –90, –60, –45 e –30°C/min), nove tempos de equilíbrio (1; 2,5; 5; 10; 15; 20; 25; 30 e 60 minutos) e cinco proporções de sêmen:diluente (1:1; 1:3; 1:6; 1:10 e 1:20) sobre a taxa de motilidade e a duração da motilidade espermáticas. Posteriormente um teste de fertilização foi realizado para avaliar a viabilidade do sêmen crioconservado. O tratamento que propiciou maior taxa de motilidade e duração da motilidade espermáticas (P<0,05) foi aquele proporcionado pelo emprego do diluente com pH 8,2 com dimetilsulfóxido a 10%, em uma velocidade de congelamento de –60°C/min, com tempo de equilíbrio de 1 minuto e na proporção de 1:3 (v/v). O sêmen crioconservado apresentou taxa de fertilização superior a 69% validando o presente protocolo para o ariocó. A crioconservação do sêmen do ariocó é uma alternativa viável, sendo possível manter uma apropriada qualidade espermática.

Animals , Male , Cryopreservation/veterinary , Cryoprotective Agents/pharmacology , Perciformes/physiology , Semen Preservation/veterinary , Sperm Motility/drug effects , Aquaculture/methods , Cryopreservation/methods , Dose-Response Relationship, Drug , Semen Preservation/methods , Time Factors
Int. j. morphol ; 32(3): 844-849, Sept. 2014. ilus
Article in English | LILACS | ID: lil-728277


Nicotine consumption can decrease fertility drive in males through inducing oxidative stress and DNA damage. The color of turmeric is because of a substance called curcumin for which some anti-oxidative and anti-inflammatory properties have been identified. In this study, various doses of curcumin (10, 30 and 60 mg/kg) and curcumin plus nicotine (10, 30 and 60 mg/kg) were administered intraperitoneally to male mice for 28 consequent days and reproductive parameters were determined. The results indicated that nicotine administration (0.5 mg/kg) significantly decreased testosterone level, count and motility of sperms, and testis weight compared to control group. However, increasing the dose of curcumin significantly increased reproductive indices in most of the groups. Thus, it seems that curcumin inhibits nicotine-induced adverse effects on reproductive parameters.

El consumo de nicotina puede disminuir la fertilidad en los hombres mediante la inducción de estrés oxidativo y daño del ADN. El color de la cúrcuma se debe a una sustancia llamada curcumina en la cual se han identificado algunas propiedades anti-oxidantes y anti-inflamatorias. En este estudio se administraron diferentes dosis de curcumina (10, 30 y 60 mg/kg) y de curcumina más nicotina (10, 30 y 60 mg/kg) por vía intraperitoneal a ratones machos durante 28 días consecutivos y se determinaron los parámetros reproductivos. La administración de nicotina (0,5 mg/kg) disminuyó significativamente el nivel de testosterona, el número y motilidad de los espermatozoides, y peso de los testículos en comparación con el grupo control. Sin embargo, el incremento de la dosis de curcumina aumentó significativamente los índices reproductivos en la mayoría de los grupos. Este estudio sugiere que la curcumina inhibe los efectos adversos inducidos por la nicotina sobre los parámetros reproductivos.

Animals , Male , Mice , Reproduction/drug effects , Testis/drug effects , Curcumin/administration & dosage , Nicotine/toxicity , Antioxidants/administration & dosage , Organ Size/drug effects , Seminiferous Tubules/drug effects , Sperm Count , Sperm Motility/drug effects , Testosterone/analysis , Curcumin/pharmacology , Antioxidants/pharmacology
Biol. Res ; 47: 1-8, 2014. graf
Article in English | LILACS | ID: biblio-950740


BACKGROUND: Nitric oxide (NO) has been shown to be important in sperm function, and the concentration of NO appears to determine these effects. Studies have demonstrated both positive and negative effects of NO on sperm function, but have not been able to provide a clear link between NO concentration and the extent of exposure to NO. To study the relationship between nitric oxide and sperm capacitationin vitro, and to provide a theoretical basis for the use of NO-related preparations in improving sperm motility for in vitro fertilization, we investigated the effects of NO concentration and time duration at these concentrations on in vitro sperm capacitation in both normal and abnormal sperm groups. We manipulated NO concentrations and the time duration of these concentrations using sodium nitroprusside (an NO donor) and NG-monomethyl-L-argenine (an NO synthase inhibitor). RESULTS: Compared to the normal sperm group, the abnormal sperm group had a longer basal time to reach the appropriate concentration of NO (p < 0.001), and the duration of time at this concentration was longer for the abnormal sperm group (p < 0.001). Both the basal time and the duration of time were significantly correlated with sperm viability and percentage of progressive sperm (p < 0.001). The experimental group had a significantly higher percentage of progressive sperm than the control group (p < 0.001). CONCLUSIONS: We hypothesize that there is a certain regularity to both NO concentration and its duration of time in regards to sperm capacitation, and that an adequate duration of time at the appropriate NO concentration is beneficial to sperm motility.

Humans , Male , Adult , Sperm Capacitation/drug effects , Sperm Motility/drug effects , Nitric Oxide/pharmacology , Time Factors , In Vitro Techniques , Nitroprusside/pharmacology , Fertilization in Vitro/methods , Cell Survival , Nitric Oxide Synthase/antagonists & inhibitors , omega-N-Methylarginine/pharmacology , Nitric Oxide/analysis
Int. j. morphol ; 31(1): 82-86, mar. 2013. ilus
Article in English | LILACS | ID: lil-676137


Cannabis Sativa is a multiuse herb in traditional medicine, its hydroalcoholic extract (10, 50, and 100 mg/kg) administered interaperitoneally for 14 consequent days to Wistar male rats resulted in significant decrease in progressive motility of sperm. Sperm count and seminiferous tubules diameter decreased significantly in comparison with control group. Also decrease in animal body weight in doses of 50 and 100 mg/kg was observed. Changes in testes weight and serum testosterone were not significant. Cannabis sativa extract has negative effect on sperm parameters such as motility, sperm count, and seminiferous tubules diameter.

La Cannabis Sativa es una hierba de múltiples usos en la medicina tradicional. Su extracto hidroalcohólico (10, 50, y 100 mg/kg) administrado intraperito-nealmente durante 14 días consecutivos a ratas Wistar macho produjo una disminución significativa en la motilidad progresiva de los espermatozoides. El recuento de espermatozoides y el diámetro de los túbulos seminíferos se redujo significativamente en comparación con el grupo control. También se observó disminución del peso corporal de los animales en dosis de 50 y 100 mg/kg. Cambios en el peso de los testículos y la testosterona sérica no fueron significativos. El extracto de Cannabis sativa tiene un efecto negativo sobre los parámetros seminales tales como la motilidad, conteo espermático, y el diámetro de los túbulos seminíferos.

Animals , Male , Rats , Spermatozoa/drug effects , Testis/drug effects , Cannabis , Plant Extracts/administration & dosage , Organ Size , Seminiferous Tubules/drug effects , Sperm Count , Sperm Motility/drug effects , Plant Extracts/pharmacology , Hydroalcoholic Solution , Rats, Wistar
IJPR-Iranian Journal of Pharmaceutical Research. 2013; 12 (2): 445-451
in English | IMEMR | ID: emr-142666


Rosmarinus officinalis has been used in traditional medicine extensively. This study evaluated the hormonal and cellular effects of Rosmarinus officinalis extract on testes of adult rats. Thirty male Wistar rats [in three groups] received 50 or 100 mg/Kg b.w of Rosmarinus officinalis extract [made from the plant's leaves, flower and stem] [treatment groups] and 10 mL/Kg b.w normal saline [control group] respectively, on a daily bases by gavage route for 60 days. Then, spermatological properties, histometric parameters and sperm dynamics, testis and body weight, testicular cell population and serum testosterone level were analyzed by an acceptable method. Results showed that the mean serum testosterone level was decreased significantly in both treatment groups [50 and 100 mg/Kg b.w] during the experiment time, compared with control group [p < 0.05]. However, Rosmarinus officinalis did not change the total count, motility and viability of sperm. In addition, Rosmarinus officinalis at both doses did not change body and testes weight and their ratio. Furthermore, Rosmarinus officinalis increased the number of Spermatogonia at both doses, Spermatocyte at doses of 50 mg/Kg b.w, Leydig cell and Spermatid at dose of 100 mg/Kg b.w significantly [p < 0.05]. Rosmarinus officinalis did not significantly affect the number of Spermatozoid and Sertoli cells. In conclusion, it seems that Rosmarinus officinalis may have some hormonal and cellular effects on the testes which can contribute the spermatogenesis process in rat. Rosmarinus officinalis may have antiandrogenic effect potentially indicating the possibility of developing herbal male contraceptive

Male , Animals, Laboratory , Sperm Motility/drug effects , Spermatogenesis/drug effects , Plant Extracts/pharmacology , Fertility/drug effects , Rats, Wistar , Infertility, Male
IJRM-Iranian Journal of Reproductive Medicine. 2013; 11 (9): 741-746
in English | IMEMR | ID: emr-130778


Caffeine increases the CAMP production that stimulates spermatozoa movement. Caffeine is also used for induction of in vitro acrosome reaction in mammalian spermatozoa, an important step in achieving fertilization. The aim of this study was to assess the effect of caffeine on sperm's motility, vitality and laboratory fertilization rates in mouse in two T6 and M16 media. Epididymal mouse sperms were collected and treated by caffine in T6 and M16 media and their motility and vitality rates were evaluated. The pretreated sperms were added to oocytes in T6 and M16 media with and without caffeine and fertilization rates were recorded after 24 hours incubation. Sperm's motility [81.7 +/- 1.67%] and vitality [88.7 +/- 1.33%] rates and percentage of fertilized oocytes [67.52 +/- 8.16%] in T6 medium plus caffeine compare to control group have increased and shown significant differences at p

Male , Female , Animals, Laboratory , Sperm Motility/drug effects , Fertilization in Vitro , Mice , Spermatozoa
Article in English | IMSEAR | ID: sea-135763


Background & objectives: Hyperprolactinaemia affects testicular functions by influencing hypothalamo-pituitary-testicular (HPT) axis at various levels. Available literature on the level of defect, time course of improvement of gonadal functions and its relation with decline in prolactin levels is scanty. We carried out this study to evaluate the HPT axis in patients with macroprolactinomas, before and six months after cabergoline therapy. Methods: Fifteen men with macroprolactinomas underwent gonadotropin and testosterone response to their respective stimuli before and after six months of cabergoline therapy. Results: Serum prolactin levels decreased after six months of therapy. Pretreatment, mean lutenizing and follicle stimulating hormones (LH and FSH) levels were 2.0 ± 0.4 and 1.4 ± 0.2 IU/l, respectively. However, LH and FSH responses to GnRH were preserved in majority of the patients and LH peaked to 12.1 ± 2.3 IU/l (P<0.01), while FSH to 2.9 ± 0.4 IU/l suggesting the influence of hyperprolactinaemia at the level of hypothalamus with preserved gonadotrope reserve. After cabergoline therapy, there was an increase in basal as well as stimulated LH and FSH levels, though these were not statistically significant when compared to respective pretherapy levels. Basal testosterone (T) levels significantly improved after therapy, but peak T response to hCG was similar at both pre- and post treatment. A significant correlation was observed between peak LH and peak T at baseline (r=0.53, P<0.01) and it further strengthened after therapy (r=0.70, P<0.01). After cabergoline therapy, there was significant improvement in seminal volume, sperm count and motility and sperm count correlated with peak FSH response (r=0.53, P<0.05). Interpretation & conclusions: Hyperprolactinaemia affects testicular functions probably by influencing at the level of hypothalamus resulting in subnormal basal secretion of gonadotropins required for optimal testicular functions.

Analysis of Variance , Enzyme-Linked Immunosorbent Assay , Ergolines/pharmacology , Ergolines/therapeutic use , Follicle Stimulating Hormone/blood , Gonadotropin-Releasing Hormone/pharmacology , Humans , Hypothalamo-Hypophyseal System/metabolism , Luteinizing Hormone/blood , Magnetic Resonance Imaging , Male , Pituitary Neoplasms/drug therapy , Pituitary Neoplasms/pathology , Prolactin/blood , Prolactinoma/drug therapy , Prolactinoma/pathology , Radioimmunoassay , Sperm Count , Sperm Motility/drug effects , Testis/metabolism , Testosterone/blood , Time Factors
Indian J Med Sci ; 2011 Feb; 65(2) 43-49
Article in English | IMSEAR | ID: sea-145589


Background : Cefpodoxime is a semisynthetic third generation cephalosporin analogue with a relatively broader spectrum of antimicrobial activity against gram negative and gram positive organisms. This is attributed to their somewhat increased resistance to degradation by the betalactamase. Cefpodoxime shows good activity against Klebsiella pneumonia, many members of enterobactericeae and almost all strains of Escherichia coli. It is extensively used in human beings against infections caused by susceptible organisms for a prolonged period and even without its judicious indication. Though various researchers have worked on the pharmacokinetic aspects of the drug, its effects on biochemical parameters and spermatozoa activity are scarcely available in literature. Aim : To determine the oral kinetic ( blood and tissue) after single therapeutic dose of cefpodoxime proxetil (20mg/kg oral bid 7 days) in rats of either sex on tissue half life and certain biochemical parameters such as glucose, hemoglobin, protein, ALT, AST and other parameters like tissue residue, sperm count and spermatozoa motility in male rats. Materials and Methods : For kinetic studies,24 Wister rats of either sex, 3 months of age, (180-210 gm) were used.(Group I-IV; n=6) Blood samples collected from each animal of Group IV through heart puncture at 0 hour to serve as predrug control. All the group (I-IV) received cefpodoxime proxetil 20 mg/kg once orally as a single dose. At the end of 1,4,12 and 24 hour post oral administration, GroupI,II,III and IVwere utilized for kinetic studies. Blood samples were collected from each animal and vital organs viz brain, lung, liver, spleen, kidney and heart were dissected out for drug analysis and determination of weight. For biochemical parameters, tissue residue and spermatozoa motility, twelve male rats were randomly divided into Groups A and B (n=6) Group B received cefpodoxime (20mg/kg orally bid 7 days) while Group A served as control. Biochemical parameters [Blood glucose, protein, Aspartate transaminase(AST), Alanine transaminase(ALT)and hemoglobin] were measured at 0 and 7 th day while sperm count (total,live and dead)and mean organ weight (study and control group) and tissue residue of drug were evaluated at the end of treatment. Absorption of cefpodoxime was observed at 2 hour and reached a maximum at 4 hour and persisted in blood till 24 hour. Elimination half life in lung was highest followed by heart, liver, kidney and spleen while t½ k in plasma was very low suggesting more affinity of cefpodoxime for tissues than blood. Results and Conclusion : Blood glucose, protein, AST and ALT activities were not significantly altered but the hemoglobin level and total and live sperm count decreased significantly in the study group compared to the control group. Residual level of cefpodoxime was highest in liver followed by kidney and other study organs. Therefore, the drug should be used in human beings judiciously and further study on human subjects is warranted.

Animal Experimentation , Animals , Animals, Newborn , Ceftizoxime/analogs & derivatives , Ceftizoxime/pharmacokinetics , Pharmacokinetics , Rats, Wistar , Sperm Motility/drug effects , Tissue Distribution/drug effects
Medical Sciences Journal of Islamic Azad University. 2011; 21 (1): 1-6
in Persian | IMEMR | ID: emr-109660


Nicotine is an important part of cigarette smoke with strong physical and psychological effects on smokers. Methylphenidate [mph] is a medicine that has been prescribed widely for treatment of attention deficit hyperactivity disorder [ADHD]. In the present study, effects of both mph and nicotine on sperm motility which is one of the most effective factors in male fertility genital tract have been evaluated. In this experimental study, one hundred and twenty male mice divided in 11 treated and one control groups. In treated groups, mph with doses of 2 and 10 mg/kg and nicotine with doses of 100, 200 and 400 micro gr/kg were prescribed separately and in combinations orally for 40 days. At the final day, mice were killed and percent of sperm motility was calculated by counting gametes present in vasodeferan. Data were analyzed using Kruskal-wallis, Post-Hoc [Scheffe] and student's t-test. P<0.05 was considered significant. The sperm motility has been reduced significantly in groups that received both mph and nicotine. it can be concluded that even in light smokers, mph administration at high amount can reduce sperm motility

Male , Animals, Laboratory , Sperm Motility/drug effects , Mice , Methylphenidate
Journal of Reproduction and Infertility. 2011; 12 (3): 201-207
in English | IMEMR | ID: emr-130084


Infertility is common among couples of child-bearing age and approximately half of known causes of primary infertility are attributable to male factor. It is still unclear whether the injurious effects of cigarette smoking on sperm characteristics and infertility are due to nicotine. Therefore, the present study investtigated the effects of orally administered of nicotine on sperm characteristics and libido in adult male albino rats. The study also sought nicotine effects on fertility rate, litter size and weight in female animals cohabited with nicotine treated male rats. Forty male and twenty-five female rats were used for the study. The male rats were divided into five groups and were treated for a period of 30 days with nicotine 0.5 mg/kg [low dose] and 1.0 mg/kg [high dose] per body weight while the control rats received 0.2 ml/kg normal saline. The fourth and fifth groups were gavaged with 0.5 mg/kg and 1.0 mg/kg body weight of nicotine but were left untreated for another 30 days. These groups served as the recovery groups. At the end of each experimental period, sperm analysis, fertility study, litter weight and size were determined. Sperm motility and count significantly decreased [P<0.05] while the percentage of abnormality significantly increased [P<0.05] in both treatment groups. However, there was an insignificant decrease [P>0.05] in the viability and semen volume of the treated groups. Fertility studies revealed that nicotine reduced libido in male rats, litter weight and number delivered by the untreated female during the experiments. The present study showed that nicotine has a dose-dependent deleterious effect on the sperm characteristics and that fertility is ameliorated by nicotine cessation in male rats

Animals, Laboratory , Male , Female , Fertility/drug effects , Nicotine/adverse effects , Rats , Sperm Motility/drug effects , Sperm Count
Journal of Reproduction and Infertility. 2011; 12 (1): 3-7
in English | IMEMR | ID: emr-131149


Active immunization against GnRH decreases the secretion of gonadotropins and causes cessation of gonadal function, thereby, inducing infertility. Based on the immunoenhancing activity of Kamdhenu ark [distilled cow urine], this study was performed to evaluate its effects on the gonadosomatic indices [GSI] and sperm parameters in male mice receiving a GnRH contraceptive vaccine. Sixty adult male mice of Parke's strain were divided into three groups of twenty. Group I served as the controls, while group II was immunized by GnRH-BSA conjugate [50/0.2/35 micro g/ml/g BW] by four intraperitoneal injections at different intervals on days 1, 30, 60 and 90. however, group III was supplemented daily by oral Kamdhenu ark [100 ppm] along with GnRH-BSA immunizations. The animals were sacrificed after 30, 60, 90 and 120 days and their testis and epididymis were dissected out weighed and semen analysis was performed. GSI values, sperm motility, sperm count and sperm morphology in male Mus musculus were decreased significantly in all the experimental groups as compared to the control group [p< 0.01]. Kamdhenu ark significantly enhanced the effect of GnRH vaccine on the aforesaid parameters especially in 90 and 120 days treated groups [p<0.05]. The changes witnessed in sperm parameters suggested that the GnRH-BSA immunization suppressed the activities of gonadotropins and testosterone directly through hypthalamo- hypophysial-gonadal axis and indirectly by acting on the testes which may modulate the sperm morphology, sperm count and motility. However, Kamdhenu ark seems to have enhanced these effects because of its immune-modulatory properties too

Male , Animals, Laboratory , Spermatozoa/drug effects , Immunization , Vaccination , Mice , Sperm Motility/drug effects , Sperm Count
Rev. méd. Chile ; 138(12): 1510-1516, dic. 2010. tab
Article in Spanish | LILACS | ID: lil-583047


Background: Semen analysis is one of the parameters used to predict male fertility. Semen can be altered by environmental pollutants; therefore it could be used as a biological marker of exposure in contaminated areas. Aim: To analyze the spermogram values in a sample of healthy young males, residing in Arica, Chile. Material and methods: One hundred and two healthy university students volunteers aged 18 to 30 years answered a questionnaire about fertility, habits and andrologic diseases and provided a semen sample. Within three hours after ejaculation, semen volume, pH, sperm concentration, motility and morphology were analyzed. Results: Six percent of volunteers had offspring, 1 percent declared to be infertile, 32 percent smoked and 78 percent con-sumed alcohol. Semen pH was 7.6 ± 0.5, volume, 2.9 ± 1.6 ml, sperm concentration, 62.8 ± 62.3 x 10(6)/ml, normal morphology, 15.0 ± 7.9 percent, overall motility, 42.2 ± 23.2 percent and grade A motility, 19.2 ± 18.6 percent. The percentage of subjects that had normal semen values was 82 percent for total sperm count, 76 percent for sperm concentration, 72 percent for volume, 64 percent for vitality, 63 percent for pH, 57 percent for morphology, 38 percent for overall motility and 26 percent for grade A motility. Conclusions: This sample of healthy young males had a normal sperm count in comparison with international reports. However it is necessary to characterize the spermogram in uncontaminated areas of Chile to ensure that our results are within the expected values for the country.

Adolescent , Adult , Humans , Male , Young Adult , Environmental Pollutants/toxicity , Fertility/drug effects , Semen Analysis , Semen/drug effects , Biomarkers/analysis , Boron/toxicity , Chile , Reproducibility of Results , Sperm Count , Semen/physiology , Sperm Motility/drug effects , Sperm Motility/physiology
Rev. Inst. Med. Trop. Säo Paulo ; 52(1): 43-46, Jan.-Feb. 2010. ilus, tab
Article in English | LILACS | ID: lil-540316


The World Health Organization (WHO) has shown concern about the burden of tuberculosis in the developing countries. Even though rifampicin is an effective drug in the management of tuberculosis, it has been documented to have some toxic effects in humans. Therefore, this study intends to investigate the modulatory effect of vitamins C and E on the hepatotoxicity, sperm quality and brain toxicity of Rifampicin. Forty Wistar albino rats were used, 10 animals per group. Group 1 animals received 0.3 mL of distilled water, the Group 2 animals received the therapeutic dose of rifampicin, Group 3 animals received therapeutic doses of rifampicin plus vitamin E, while Group 4 received therapeutic doses of rifampicin and vitamin C. The administration was performed orally during three months; the animals were sacrificed by cervical dislocation at the end of that period. Blood samples were collected and liver function and lipid profile was analyzed using fully automated clinical chemistry device. The liver, brain and reproductive organs underwent histopathological examination. Sperm samples were collected from the epididymis to achieve count and motility and morphological analysis. Results showed rifampicin alone to raise (p < 0.05) liver function enzymes (Aspartate amino transferase [AST], Serum alanine amino transferase [ALT] and Total Bilirubin) when compared with controls. While the vitamin E treated group showed remarkable protection, the vitamin C treated group showed questionable protection against the rifampicin induced liver damage. Sperm count results showed an important (p < 0.05) increase in the sperm quality in vitamin E and C treated groups. However, the vitamin E plus Rifampicin treated group showed increased lipid peroxidation. The histopathological findings revealed structural damages by rifampicin in liver, brain and epididymis while some remarkable architectural integrity was observed in the antioxidant-treated groups. It can be ...

A Organização Mundial da Saúde tem mostrado preocupação acerca da eclosão da tuberculose nos países em desenvolvimento. Embora a rifampicina seja droga efetiva para o controle da tuberculose têm sido documentados seus efeitos tóxicos em pacientes. Portanto este estudo tem a intenção de investigar o efeito modulador das vitaminas C e E na hepatotoxicidade, qualidade de esperma e a toxicidade cerebral da rifampicina. Quarenta ratos albinos da raça Wistar foram usados, 10 animais por grupo. O grupo 1 de animais recebeu 0,3 mL de água destilada. O grupo 2 recebeu a dose terapêutica de rifampicina. O grupo 3 recebeu doses terapêuticas de rifampicina mais vitamina E, enquanto o grupo 4 recebeu doses terapêuticas de rifampicina e vitamina C. A administração foi feita por via oral durante três meses; os animais foram sacrificados por deslocação cervical após este período. Amostras sanguíneas foram coletadas e função hepática e o perfil lipídico foram analisados usando aparelho automático de química clínica. O fígado, o cérebro e os órgãos reprodutivos foram submetidos a análise histopatológica. As amostras de esperma foram coletadas do epidídimo para contagem, motilidade e análise morfológica. Resultados revelaram que a rifampicina isoladamente aumenta (p < 0,05) os enzimas de função hepática (aspartato amino transferase {AST], alanino amino transferase sérica [ALT] e bilirrubina total) quando comparados com os controles. Embora o grupo tratado com vitamina E mostrasse marcada proteção, o grupo tratado com vitamina C mostrou proteção questionável contra a lesão hepática induzida pela rifampicina. Resultados da contagem espermática mostraram importante (p < 0,05) aumento na qualidade do esperma no grupo tratado com vitamina E e C. Entretanto, o grupo tratado com vitamina E e rifampicina mostrou aumento da peroxidação lipídica. Os achados histopatológicos revelaram danos estruturais pela rifampicina ao fígado, cérebro e epidídimo enquanto uma notável ...

Animals , Male , Rats , Antibiotics, Antitubercular/toxicity , Antioxidants/pharmacology , Brain/drug effects , Rifampin/toxicity , Spermatozoa/drug effects , Alanine Transaminase/metabolism , Antibiotics, Antitubercular/antagonists & inhibitors , Ascorbic Acid/pharmacology , Aspartate Aminotransferases/metabolism , Liver/drug effects , Liver/enzymology , Rats, Wistar , Rifampin/antagonists & inhibitors , Sperm Count , Sperm Motility/drug effects , Vitamin E/pharmacology