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1.
Arq. bras. oftalmol ; 83(2): 160-167, Mar.-Apr. 2020. tab, graf
Article in English | LILACS | ID: biblio-1088965

ABSTRACT

ABSTRACT Degenerative retinal diseases such as retinitis pigmentosa, Stargardt's macular dystrophy, and age-related macular degeneration are characterized by irreversible loss of vision due to direct or indirect photoreceptor damage. No effective treatments exist, but stem cell studies have shown promising results. Our aim with this review was to describe the types of stem cells that are under study, their effects, and the main clinical trials involving them.


RESUMO As doenças degenerativas da retina, como retinose pigmentar, distrofia macular de Stargardt e degeneração macular relaciona à idade, são caracterizadas por perda irre versível da visão devido a danos diretos ou indiretos aos fotorreceptores. Não existem tratamentos eficazes, porém os estudos com células-tronco mostraram resultados promissores. Nosso objetivo com esta revisão foi descrever os tipos de células-tronco em estudo, seus efeitos e os principais ensaios clínicos que as envolvem.


Subject(s)
Humans , Retinal Degeneration/therapy , Pluripotent Stem Cells/transplantation , Stem Cell Transplantation/methods , Retina/cytology , Clinical Trials as Topic , Treatment Outcome
3.
Biol. Res ; 52: 16, 2019. tab, graf
Article in English | LILACS | ID: biblio-1011418

ABSTRACT

BACKGROUND: Sperm production is one of the most complex biological processes in the body. In vitro production of sperm is one of the most important goals of researches in the field of male infertility treatment, which is very important in male cancer patients treated with gonadotoxic methods and drugs. In this study, we examine the progression of spermatogenesis after transplantation of spermatogonial stem cells under conditions of testicular tissue culture. RESULTS: Testicular tissue samples from azoospermic patients were obtained and then these were freeze-thawed. Spermatogonial stem cells were isolated by two enzymatic digestion steps and the identification of these cells was confirmed by detecting the PLZF protein. These cells, after being labeled with DiI, were transplanted in azoospermia adult mice model. The host testes were placed on agarose gel as tissue culture system. After 8 weeks, histomorphometric, immunohistochemical and molecular studies were performed. The results of histomorphometric studies showed that the mean number of spermatogonial cells, spermatocytes and spermatids in the experimental group was significantly more than the control group (without transplantation) (P < 0.05) and most of the cells responded positively to the detection of DiI. Immunohistochemical studies in host testes fragments in the experimental group express the PLZF, SCP3 and ACRBP proteins in spermatogonial cells, spermatocyte and spermatozoa, respectively, which confirmed the human nature of these cells. Also, in molecular studies of PLZF, Tekt1 and TP1, the results indicated that the genes were positive in the test group, while not in the control group. CONCLUSION: These results suggest that the slow freezing of SSCs can support the induction of spermatogenesis to produce haploid cells under the 3-dimensional testicular tissue culture.


Subject(s)
Humans , Animals , Male , Mice , Spermatogenesis/physiology , Spermatogonia/transplantation , Testis/cytology , Cryopreservation/methods , Stem Cell Transplantation/methods
4.
Int. j. morphol ; 37(1): 349-357, 2019. graf
Article in English | LILACS | ID: biblio-990050

ABSTRACT

SUMMARY: The aim of this study was to determine the possible regenerative effect of neuroectodermal stem cells on the ultrastructural, and locomotor function resulting from compressed injury to the spinal cord in a rat model. Forty male rats were divided into control and sham groups (20 rats each). Compressed spinal cord injured (CSCI) were forty rats which subdivided equally into: untreated, treated by neuroectodermal stem cells (NESCs). After four weeks, all rats in different groups were scarified, samples were taken from central, cranial, and caudal to the site of spinal cord injury. Specimens were prepared for light and electron microscopic examination. The number of remyelinated axons in central, cranial and caudal regions to the injured spinal cord after transplantation of NESCs was counted. The open field test assessed the locomotor function. Results revealed that compressed spinal cord injury resulted in loss and degeneration of numerous nerve fibers, myelin splitting and degeneration of mitochondria. Four weeks after transplantation of NESCs regenerated axons were noticed in cranial and central sites, while degenerate axons were noticed caudal to the lesion. Number of remyelinated axons was significantly increased in both central and cranial to the site of spinal cord injury in comparison with caudal region which had the least number of remyelinated axons. Transplantation of NESCs improved significantly the locomotor functional activity In conclusion, neuroectodermal stem cells transplantation ameliorated the histopathological and ultrastructural changes, and improved the functional locomotor activity in CSCI rat.


RESUMEN: El objetivo de este estudio fue determinar el posible efecto regenerativo de las células madre neuroectodérmicas en la función ultraestructural y locomotora de una lesión comprimida en la médula espinal en un modelo de rata. Cuarenta ratas macho se dividieron en grupos control y sham (20 ratas en cada grupo). La médula espinal lesionada (CSCI) tenía cuarenta ratas que se subdividieron de igual forma en los siguientes grupos: no tratadas, tratadas con células madre neuroectodérmicas (NESCs). Al término de cuatro semanas, todas las ratas en los diferentes grupos fueron escarificadas, se tomaron muestras de las áreas central, craneal y caudal en relación al sitio de la lesión de la médula espinal. Las muestras fueron preparadas para examen microscópico de luz y electrónica. Se contó el número de axones remielinizados en las regiones central, craneal y caudal de la médula espinal lesionada después del trasplante de NESCs. La prueba de campo abierto evaluó la función locomotora. Los resultados revelaron que la lesión de la médula espinal comprimida provocó la pérdida y degeneración de numerosas fibras nerviosas, la división de la mielina y la degeneración de las mitocondrias. Cuatro semanas después del trasplante de NESCs, se notaron axones regenerados en los sitios craneales y centrales, mientras que los axones degenerados se notaron caudal a la lesión. El número de axones remielinizados aumentó significativamente tanto en el centro como en el cráneo hasta el sitio de la lesión de la médula espinal en comparación con la región caudal que tenía el menor número de axones remielinizados. El trasplante de NESCs mejoró significativamente la actividad funcional locomotora. En conclusión, el trasplante de células madre neuroectodérmicas mejoró los cambios histopatológicos y ultraestructurales, y mejoró la actividad locomotora funcional en la rata CSCI.


Subject(s)
Animals , Female , Rats , Spinal Cord Injuries/therapy , Stem Cell Transplantation/methods , Nerve Regeneration/physiology , Spinal Cord/ultrastructure , Axons , Motor Activity
5.
Rev. cuba. hematol. inmunol. hemoter ; 34(2): 143-152, abr.-jun. 2018. tab
Article in Spanish | LILACS, CUMED | ID: biblio-978420

ABSTRACT

Introducción: La lesión de la médula espinal es una condición devastadora que se produce por traumatismos raquimedulares con alta morbilidad y mortalidad, para la cual no existe un tratamiento efectivo disponible mediante las terapéuticas convencionales. En la actualidad, se han desarrollado algunas estrategias neurorregenerativas, entre las que se encuentra la implantación de células madre. Este proceder ha creado nuevas expectativas en la búsqueda de un tratamiento efectivo para este tipo de lesión. Objetivo: Evaluar la factibilidad y seguridad de la implantación en la médula espinal lesionada de células madre autólogas derivadas de la médula ósea. Métodos: Las células madre autólogas provenientes de la médula ósea se implantaron a cielo abierto en el sitio de la lesión; combinadas o no con citocinas neuroestimulantes (factor estimulador de colonias de granulocitos o eritropoyetina recombinante) administradas por vía sistémica. Resultados: Se incluyeron 25 pacientes adultos con lesión completa de la médula espinal secundaria a traumatismos raquimedulares. El mecanismo de producción más frecuente fue el accidente de tráfico. Después del tratamiento se observaron evolutivamente en los pacientes cambios sensitivos y motores, más significativos en los casos tratados con células madre asociadas con las citocinas neuroestimulantes. Conclusiones: Se demostró la factibilidad y seguridad del implante celular y su mayor efectividad en asociación con citocinas neuroestimulantes, sin complicaciones de importancia(AU)


Introduction: Spinal cord injury is a devastating condition caused by spinal cord injury with high morbidity and mortality, and for which there is no effective treatment available through conventional treatments. At present, some neuroregenerative strategies have been proposed, among them the implantation of stem cells. This procedure has created new expectations in the search for an effective treatment for this type of injury. Objective: To evaluate the feasibility and safety of implantation in the injured spinal cord of autologous stem cells derived from the bone marrow. Methods: Autologous stem cells from the bone marrow were implanted at open-air into the site of the lesion, combined or not with systemically administration of the neurostimulant cytokines (granulocyte colony-stimulating factor and recombinant erythropoietin). Results: Twenty-five adult patients with chronic complete spinal cord injury were included. The most frequent mechanism of production was the traffic accident. After treatment, many significant evolutionary changes in the sensory and motor evolution, were observed in the patients treated with stem cells combined with the neurostimulant cytokines. Conclusions: The feasibility and safety of the cellular implant and its association with neurostimulant cytokines were demonstrated, since there were no major complications(AU)


Subject(s)
Humans , Spinal Cord Injuries/therapy , Spinal Cord Injuries/epidemiology , Blood Specimen Collection/methods , Stem Cell Transplantation/methods
6.
Arch. cardiol. Méx ; 88(5): 483-495, dic. 2018. graf
Article in Spanish | LILACS | ID: biblio-1142160

ABSTRACT

Resumen Actualmente las enfermedades cardiovasculares se han convertido en un serio problema para los sistemas de salud de todo el mundo, ya que son la principal causa de muerte y representan una enorme carga económica. Este problema ha sido abordado con diferentes estrategias, entre ellas con la ayuda de terapia celular, aunque sin resultados contundentes. Durante más de 20 años, se ha utilizado una gran variedad de células madre en diferentes modelos de infarto del miocardio. El uso de células madre cardiacas (CSC) parece ser la mejor opción, pero la inaccesibilidad y la escasez de estas células hacen que su uso sea muy limitado. Además, existe un riesgo elevado pues tienen que obtenerse directamente del corazón del paciente. A diferencia de las CSC, las células madre adultas derivadas de médula ósea o tejido adiposo, entre otras, representan una opción atractiva debido a su fácil accesibilidad y abundancia, pero sobre todo a la probable existencia de progenitores cardiacos entre sus diferentes subpoblaciones. En esta revisión hacemos un análisis de los marcadores de superficie presentes en CSC en comparación con otras células madre adultas, y sugerimos la preexistencia de células que comparten marcadores de superficie específicos con CSC, la presencia de un inmunofenotipo predecible, aunque en proporciones bajas, pero con un potencial de diferenciación cardiaca similar a las CSC, lo cual podría aumentar su valor terapéutico. Este estudio revela las nuevas perspectivas con respecto a la presencia de dichos marcadores, los cuales comprometerían algunas de estas subpoblaciones a diferenciarse a tejido cardiaco.


Abstract It is well-known that cardiovascular diseases are the leading cause of death world- wide, and represent an important economic burden to health systems. In an attempt to solve this problem, stem cell therapy has emerged as a therapeutic option. Within the last 20 years, a great variety of stem cells have been used in different myocardial infarction models. Up until now, the use of cardiac stem cells (CSCs) has seemed to be the best option, but the inaccessibility and scarcity of these cells make their use unreliable. Additionally, there is a high risk as they have to be obtained directly from the heart of the patient. Unlike CSCs, adult stem cells originating from bone marrow or adipose tissue, among others, appear to be an attractive option due to their easier accessibility and abundance, but particularly due to the probable existence of cardiac progenitors among their different sub-populations. In this review an analysis is made of the surface markers present in CSCs compared with other adult stem cells. This suggested the pre-existence of cells sharing specific surface markers with CSCs, a predictable immunophenotype present in some cells, although in low proportions, and with a potential of cardiac differentiation that could be similar to CSCs, thus increasing their therapeutic value. This study highlights new perspectives regarding MSCs that would enable some of these sub-populations to be differentiated at cardiac tissue level.


Subject(s)
Humans , Animals , Stem Cells/cytology , Cardiovascular Diseases/therapy , Stem Cell Transplantation/methods , Cardiovascular Diseases/physiopathology , Cell Differentiation/physiology , Immunophenotyping , Myocardial Infarction/physiopathology , Myocardial Infarction/therapy
7.
Medwave ; 18(5): e7241, 2018.
Article in English, Spanish | LILACS | ID: biblio-915444

ABSTRACT

Resumen INTRODUCCIÓN: Muchos pacientes con enfermedad de Parkinson tienen una respuesta limitada con el tratamiento farmacológico convencional. Se ha postulado el uso de células madre como una alternativa, aunque su efectividad sigue siendo un tema de controversia. MÉTODOS: Para responder esta pregunta utilizamos Epistemonikos, la mayor base de datos de revisiones sistemáticas en salud, la cual es mantenida mediante búsquedas en múltiples fuentes de información, incluyendo MEDLINE, EMBASE, Cochrane, entre otras. Extrajimos los datos desde las revisiones identificadas, reanalizamos los datos de los estudios primarios, realizamos un metanálisis y preparamos una tabla de resumen de los resultados utilizando el método GRADE. RESULTADOS Y CONCLUSIONES: Identificamos dos revisiones sistemáticas que en conjunto incluyeron 21 estudios primarios, de los cuales tres corresponden a ensayos aleatorizados. Concluimos que no está claro si las células madre podrían tener algún efecto sobre la sintomatología de la enfermedad de Parkinson porque la certeza de la evidencia disponible es muy baja.


Abstract INTRODUCTION: There are many patients with Parkinson's disease who have a limited response to conventional pharmacological treatment. The use of stem cells has been postulated as an alternative, although its effectiveness remains a matter of controversy. METHODS: To answer this question we used Epistemonikos, the largest database of systematic reviews in health, which is maintained by screening multiple information sources, including MEDLINE, EMBASE, Cochrane, among others. We extracted data from the systematic reviews, reanalyzed data of primary studies, conducted a meta-analysis and generated a summary of findings table using the GRADE approach. RESULTS AND CONCLUSIONS: We identified two systematic reviews including 21 studies overall, of which three were randomized trials. We concluded it is not clear whether stem cells have any effect on the symptoms of Parkinson's disease because the certainty of the available evidence is very low.


Subject(s)
Humans , Parkinson Disease/therapy , Stem Cell Transplantation/methods , Parkinson Disease/physiopathology , Randomized Controlled Trials as Topic , Databases, Factual , Treatment Outcome
8.
Acta cir. bras ; 32(7): 540-549, July 2017. tab, graf
Article in English | LILACS | ID: biblio-886216

ABSTRACT

Abstract Purpose: To investigate the therapeutic potential of human immature dental pulp stem cells in the treatment of chronic spinal cord injury in dogs. Methods: Three dogs of different breeds with chronic SCI were presented as animal clinical cases. Human immature dental pulp stem cells were injected at three points into the spinal cord, and the animals were evaluated by limb function and magnetic resonance imaging (MRI) pre and post-operative. Results: There was significant improvement from the limb function evaluated by Olby Scale, though it was not supported by the imaging data provided by MRI and clinical sign and evaluation. Conclusion: Human dental pulp stem cell therapy presents promising clinical results in dogs with chronic spinal cord injuries, if used in association with physical therapy.


Subject(s)
Humans , Animals , Dogs , Spinal Cord Injuries/veterinary , Stem Cell Transplantation/veterinary , Dental Pulp/cytology , Dog Diseases/therapy , Spinal Cord Injuries/therapy , Magnetic Resonance Imaging , Chronic Disease , Treatment Outcome , Recovery of Function , Stem Cell Transplantation/methods
9.
J. appl. oral sci ; 25(1): 10-19, Jan.-Feb. 2017. tab, graf
Article in English | LILACS, BBO | ID: biblio-841166

ABSTRACT

Abstract Objectives This study aimed to evaluate the potential of adipose-derived stem cells (ASCs) combined with a modified α-tricalcium phosphate (α-TCP) or gelatin sponge (GS) scaffolds for bone healing in a rat model. Material and Methods Bone defects were surgically created in the femur of adult SHR rats and filled with the scaffolds, empty or combined with ASCs. The results were analyzed by histology and histomorphometry on days seven, 14, 30, and 60. Results Significantly increased bone repair was observed on days seven and 60 in animals treated with α-TCP/ASCs, and on day 14 in the group treated with GS/ASCs, when compared with the groups treated with the biomaterials alone. Intense fibroplasia was observed in the group treated with GS alone, on days 14 and 30. Conclusions Our results showed that the use of ASCs combined with α-TCP or GS scaffolds resulted in increased bone repair. The higher efficacy of the α-TCP scaffold suggests osteoconductive property that results in a biological support to the cells, whereas the GS scaffold functions just as a carrier. These results confirm the potential of ASCs in accelerating bone repair in in vivo experimental rat models. These results suggest a new alternative for treating bone defects.


Subject(s)
Animals , Male , Biocompatible Materials/pharmacology , Bone Regeneration/drug effects , Calcium Phosphates/pharmacology , Adipose Tissue/cytology , Stem Cell Transplantation/methods , Tissue Scaffolds , Gelatin Sponge, Absorbable/pharmacology , Osteogenesis/drug effects , Rats, Inbred SHR , Tetrazolium Salts , Time Factors , Wound Healing/drug effects , Biocompatible Materials/therapeutic use , Calcium Phosphates/therapeutic use , Cell Adhesion/drug effects , Cells, Cultured , Reproducibility of Results , Treatment Outcome , Models, Animal , Cell Proliferation/drug effects , Femur/surgery , Femur/pathology , Fibroblasts/drug effects , Formazans , Gelatin Sponge, Absorbable/therapeutic use
10.
Int. j. morphol ; 34(4): 1531-1538, Dec. 2016. ilus
Article in Spanish | LILACS | ID: biblio-840919

ABSTRACT

El propósito de este estudio fue evaluar la evidencia en relación al empleo de técnicas reconstructivas en el área cráneo-facial asociado el uso de células troncales en humanos. Se realizó una revisión sistemática de la literatura en las bases Medline, ScienceDirect, EMBASE, TripDatabase, LILACS entre marzo del 2004 a marzo del 2016 con criterios de elegibilidad y estrategia de búsqueda definida. La selección de artículos fue realizada por dos investigadores de forma independiente y cuando ellos presentaron discordancia, un tercer investigador realizó le selección. Se encontraron un total de 382 artículos, se realizó una selección de artículos, eliminado duplicados, estudios experimentales en animales y selección según análisis de resúmenes, se seleccionaros 14 artículos con procedimientos reconstructivos en el área cráneo-facial asociado a células troncales. Existe limitada evidencia de calidad en relación a la utilización de células troncales en reconstrucción cráneo facial en humanos. A excepción de un estudio (ensayo clínico de bajo nivel de evidencia), todos corresponden a series o reporte de casos, con lo cual no es posible recomendar su utilización en procedimientos reconstructivos. Es necesario realizar estudios de evidencia sustentable con el empleo de células troncales que permitan identificar su real rendimiento al compararlo con otras técnicas quirúrgicas.


The purpose of this study was to evaluate the evidence regarding the use of reconstructive techniques in the craniofacial area associated with the use of stem cells in humans. A systematic review of the literature was conducted in the databases Medline, ScienceDirect, EMBASE, TripDatabase, LILACS between March 2004 and March 2016 with eligibility criteria and defined search strategy. The selection of articles was done by two researchers independently and when they presented discordance, a third researcher made the selection. We found a total of 382 articles, made a selection of articles, removed duplicates, experimental studies in animals and selection according to analysis of abstracts, we selected 14 articles with reconstructive procedures in the craniofacial area associated with stem cells. There is limited quality evidence regarding the use of stem cells in the craniofacial reconstruction in humans. With the exception of one study (clinical trial of low level of evidence), all correspond to series or report of cases, with which it is not possible to recommend its use in reconstructive procedures. It is necessary to carry out studies of sustainable evidence with the use of stem cells that allow to identify its real performance when compared with other surgical techniques.


Subject(s)
Humans , Oral Surgical Procedures/methods , Stem Cell Transplantation/methods , Tissue Engineering , Adult Stem Cells , Reconstructive Surgical Procedures/methods
11.
Rev. bras. cir. cardiovasc ; 31(6): 465-467, Nov.-Dec. 2016. tab, graf
Article in English | LILACS | ID: biblio-1042026

ABSTRACT

Abstract Validation of transendocardial injection as a method for delivering therapeutic agents to the diseased heart is increasing. Puncture heart biopsies should re-emerge as a possible alternative method to allow access to the myocardium and implantable biomaterial for cell therapy. Therefore, this work aims to present a percutaneous puncture device for biopsy and intramyocardial biomaterial injection, standardize the technique and attest to the safety of the method. The adaptation consists of creating myocardial microlesions that allow for better fixation of stem cells. The objective of this technical note covers only the development of the needle and the histological quality of the biopsies. It has not been used in humans yet.


Subject(s)
Humans , Animals , Biopsy, Needle/methods , Stem Cell Transplantation/methods , Myocardium , Needles , Biopsy, Needle/instrumentation , Reproducibility of Results , Stem Cell Transplantation/instrumentation
12.
Rev. cuba. hematol. inmunol. hemoter ; 32(3): 375-387, jul.-set. 2016. ilus, tab
Article in Spanish | LILACS | ID: biblio-844885

ABSTRACT

Introducción : Existe un creciente interés científico en el potencial terapéutico de las células madre mesenquimales derivadas de tejido adiposo ( ADSCs, en inglés). Estas células son abundantes en el tejido adiposo, son de fácil obtención y con un alto potencial de diferenciación hacia linajes celulares especializados incluyendo adipocitos, osteocitos, condrocitos, miocitos, cardiomiocitos, tenocitos, vasos sanguíneos y neuronas. Este trabajo se desarrolló con el objetivo de implementar en el laboratorio un procedimiento para aislar y cultivar ADSCs, con características que corresponden a las informadas para este linaje celular. Método: los precursores de células adiposas humanas se obtuvieron de tejido subcutáneo abdominal. Las células se separaron enzimáticamente del tejido y se decantaron por centrifugación, luego de cultivadas, se caracterizaron en su capacidad de diferenciación y por su marcadores fenotípicos. Resultados: Las ADSCs aisladas se replicaron en estas condiciones de cultivo y mantuvieron un fenotipo estable durante todo el período de estudio. Se comprobó su potencial adipogénico y osteogénico in vitro, como corresponde a las células madre mesenquimales. El estudio por citometría de flujo mostró que estas células expresan CD73, CD90 y CD105 y son negativas para los marcadores de linaje hematopoyético CD34 y CD45.En los ensayos de inhibición in vitro, las ADSCs demostraron su capacidad para inhibir la proliferación de células T humanas. Conclusiones : La caracterización fenotípica y funcional de las ADSCs obtenidas a partir del tejido adiposo abdominal demuestra que es posible la obtención mediante cultivo in vitro de células mesenquimales humanas sin inducir diferenciación espontánea, manteniendo su integridad funcional y altos niveles de proliferación, lo que sienta las bases para el inicio de ensayos preclínicos y su uso futuro en la terapia celular en nuestro país(AU)


Introduction : There is growing scientific interest in the therapeutic potential of stem cells derived from adipose tissue (ADSCs). These cells are abundant in adipose tissue, are readily available and have a high potential fordifferentiation into specialized cell lineages including adipocytes, osteocytes, chondrocytes, myocytes, cardiomyocytes, tenocyte, endothelial cells and neurons. The aim of this work was to isolate, cultivate andcharacterize ADSCs. Methods : human adipose precursor cells were obtained from abdominal subcutaneous tissue. Cells were enzymatically separated from the tissue and decanted by centrifugation, cultured and finally analyzed. Results : The ADSCs were able to replicate in our culture conditions. The cells maintained their phenotype in different passages throughout the study period, confirming its feasibility for in vitro culture. Also the ADSCs were induced to adipogenic and osteogenic differentiation, verifying its potential as mesenchymal stem cells in vitro. The flow cytometric study showed that these cells expressed CD73, CD90 and CD105 (markers of mesenchymal cells) and they were negative for CD34 and CD45 (hematopoieticcell markers). The ADSCs were able to inhibit in vitro the proliferation of T cells. Conclusions : It is possible to obtain ADSCs by in vitro cultivation without adipogenic induction, maintaining its functional integrity and high proliferation; this cell could be an important tool for the cellular therapy in our country(AU)


Subject(s)
Humans , Female , Abdominal Fat/transplantation , Mesenchymal Stem Cell Transplantation/methods , Stem Cell Transplantation/methods , Flow Cytometry/methods
13.
Arq. bras. oftalmol ; 79(2): 116-118, Mar.-Apr. 2016. tab, graf
Article in English | LILACS | ID: lil-782805

ABSTRACT

ABSTRACT This study aimed to evaluate the effectiveness of the novel simple limbal epithelial transplantation (SLET) technique, which reduces the risk of iatrogenic limbal stem cell deficiency in the donor eye. Four patients with total unilateral limbal stem cell deficiency received a limbal graft, measuring 4 mm × 2 mm, from the contralateral healthy eye in a single surgical procedure. The graft was divided into 10-20 pieces and distributed on the corneal surface. At 6-month follow-up, a completely avascular corneal epithelial surface was obtained in two patients, and there was improvement in visual acuity in one patient. The limbal grafts did not adhere to the cornea in one patient. No serious complications related to the surgery were observed in this study.


RESUMO Este trabalho tem como objetivo avaliar a eficácia de uma nova técnica cirúrgica denominada SLET (simple limbal epithelial transplantation), um procedimento promissor que reduz os riscos de indução de deficiência límbica iatrogênica no olho doador. Quatro pacientes com deficiência límbica total unilateral, secundária a queimadura química, receberam um enxerto de células límbicas, medindo 4 mm x 2 mm, do olho contralateral sadio, em apenas um tempo cirúrgico. Este foi divido em 10 a 20 fragmentos e distribuído sobre a superfície da córnea. Após 6 meses de cirurgia, superfície corneana totalmente epitelizada e avascular foi obtida em dois pacientes. Houve melhora da acuidade visual em um paciente. Não houve aderência dos enxertos de limbo na córnea em um paciente. Nenhum paciente apresentou efeitos colaterais graves decorrentes do procedimento cirúrgico.


Subject(s)
Humans , Male , Female , Adolescent , Adult , Middle Aged , Aged , Eye Burns/surgery , Limbus Corneae/cytology , Epithelium, Corneal/transplantation , Corneal Diseases/surgery , Stem Cell Transplantation/methods , Postoperative Period , Transplantation, Autologous/methods , Visual Acuity , Treatment Outcome , Epithelium, Corneal/cytology
14.
Braz. j. med. biol. res ; 49(9): e5319, 2016. tab, graf
Article in English | LILACS | ID: biblio-951695

ABSTRACT

Spinal cord injury (SCI) is a disabling condition resulting in deficits of sensory and motor functions, and has no effective treatment. Considering that protocols with stem cell transplantation and treadmill training have shown promising results, the present study evaluated the effectiveness of stem cells from human exfoliated deciduous teeth (SHEDs) transplantation combined with treadmill training in rats with experimental spinal cord injury. Fifty-four Wistar rats were spinalized using NYU impactor. The rats were randomly distributed into 5 groups: Sham (laminectomy with no SCI, n=10); SCI (laminectomy followed by SCI, n=12); SHEDs (SCI treated with SHEDs, n=11); TT (SCI treated with treadmill training, n=11); SHEDs+TT (SCI treated with SHEDs and treadmill training; n=10). Treatment with SHEDs alone or in combination with treadmill training promoted functional recovery, reaching scores of 15 and 14, respectively, in the BBB scale, being different from the SCI group, which reached 11. SHEDs treatment was able to reduce the cystic cavity area and glial scar, increase neurofilament. Treadmill training alone had no functional effectiveness or tissue effects. In a second experiment, the SHEDs transplantation reduced the TNF-α levels in the cord tissue measured 6 h after the injury. Contrary to our hypothesis, treadmill training either alone or in combination, caused no functional improvement. However, SHEDs showed to be neuroprotective, by the reduction of TNF-α levels, the cystic cavity and the glial scar associated with the improvement of motor function after SCI. These results provide evidence that grafted SHEDs might be an effective therapy to spinal cord lesions, with possible anti-inflammatory action.


Subject(s)
Humans , Animals , Male , Physical Conditioning, Animal/methods , Spinal Cord Injuries/therapy , Stem Cell Transplantation/methods , Dental Pulp/cytology , Exercise Therapy/methods , Time Factors , Tooth Exfoliation , Enzyme-Linked Immunosorbent Assay , Random Allocation , Treatment Outcome , Rats, Wistar , Combined Modality Therapy , Recovery of Function , Flow Cytometry , Locomotion
15.
Braz. j. med. biol. res ; 49(4): e5064, 2016. tab, graf
Article in English | LILACS | ID: biblio-951670

ABSTRACT

In mammals, damage to sensory receptor cells (hair cells) of the inner ear results in permanent sensorineural hearing loss. Here, we investigated whether postnatal mouse inner ear progenitor/stem cells (mIESCs) are viable after transplantation into the basal turns of neomycin-injured guinea pig cochleas. We also examined the effects of mIESC transplantation on auditory functions. Eight adult female Cavia porcellus guinea pigs (250-350g) were deafened by intratympanic neomycin delivery. After 7 days, the animals were randomly divided in two groups. The study group (n=4) received transplantation of LacZ-positive mIESCs in culture medium into the scala tympani. The control group (n=4) received culture medium only. At 2 weeks after transplantation, functional analyses were performed by auditory brainstem response measurement, and the animals were sacrificed. The presence of mIESCs was evaluated by immunohistochemistry of sections of the cochlea from the study group. Non-parametric tests were used for statistical analysis of the data. Intratympanic neomycin delivery damaged hair cells and increased auditory thresholds prior to cell transplantation. There were no significant differences between auditory brainstem thresholds before and after transplantation in individual guinea pigs. Some mIESCs were observed in all scalae of the basal turns of the injured cochleas, and a proportion of these cells expressed the hair cell marker myosin VIIa. Some transplanted mIESCs engrafted in the cochlear basilar membrane. Our study demonstrates that transplanted cells survived and engrafted in the organ of Corti after cochleostomy.


Subject(s)
Animals , Female , Organ of Corti/surgery , Stem Cells , Stem Cell Transplantation/methods , Hair Cells, Auditory, Inner/transplantation , Hearing Loss, Sensorineural/surgery , Auditory Threshold , Immunohistochemistry , Protein Synthesis Inhibitors , Neomycin , Cell Survival , Cells, Cultured , Reproducibility of Results , Evoked Potentials, Auditory, Brain Stem , Treatment Outcome , Guinea Pigs , Mice, Inbred BALB C
16.
Braz. oral res. (Online) ; 30(1): e20, 2016. tab, graf
Article in English | LILACS | ID: biblio-951968

ABSTRACT

Abstract The aim of this study was to evaluate whether medium modification improves the odontogenic differentiation of human dental pulp stem cells (DPSC) in vitro and in vivo. DPSC isolated from human impacted third molar teeth were analysed for clusters of differentiation with flow cytometry. Odontogenic differentiation was stimulated by medium modification with the addition of bone morphogenetic protein 2 (BMP2). The expression of dentin sialophosphoprotein, dentin matrix protein 1, enamelysin/matrix metalloproteinase 20 and the phosphate-regulating gene with homologies to endopeptidases on the X chromosome of the cells were analysed with RT-PCR at 7, 14 and 21 days. Then, DPSC were transplanted on the back of immunocompromised mice via a hydroxyapatite tricalcium phosphate scaffold, and the structure of the formed tissue was investigated. The cells were identified as mesenchymal stem cells with a 98.3% CD73 and CD90 double-positive cell rate. The increase in mineralization capacity and expression of human enamel-dentin specific transcripts proportional to the culture period were determined after differentiation. Six weeks after transplantation, an osteo-dentin matrix was formed in the group in which odontogenic differentiation was stimulated, and the odontogenic characteristics of the matrix were confirmed by histological examination and RT-PCR analysis. Odontogenic differentiation of the isolated and characterized human DPSC was improved with medium modification by the addition of BMP2 in vitro and in vivo. The defined medium and applied technique have a potential use for forming reparative dentin in the future, but the effects of the method should be investigated in long-term studies.


Subject(s)
Humans , Animals , Adult , Mice , Young Adult , Stem Cells/cytology , Cell Differentiation/drug effects , Culture Media/chemistry , Dental Pulp/cytology , Bone Morphogenetic Protein 2/pharmacology , Phosphoproteins/analysis , Sialoglycoproteins/analysis , Time Factors , Cell Differentiation/physiology , Cells, Cultured , Reproducibility of Results , Extracellular Matrix Proteins/analysis , Actins/analysis , Reverse Transcriptase Polymerase Chain Reaction , Stem Cell Transplantation/methods , Cell Proliferation/drug effects , Cell Proliferation/physiology , Matrix Metalloproteinase 20/analysis , PHEX Phosphate Regulating Neutral Endopeptidase/analysis , Bone Morphogenetic Protein 2/chemistry , Flow Cytometry , Odontogenesis/drug effects , Odontogenesis/physiology
17.
Yonsei Medical Journal ; : 1152-1158, 2016.
Article in English | WPRIM | ID: wpr-34049

ABSTRACT

PURPOSE: We report our initial experience with transurethral injection of autologous adipose-derived regenerative cells (ADRCs) for the treatment of urinary incontinence after radical prostatectomy. MATERIALS AND METHODS: After providing written informed consent, six men with persistent urinary incontinence after radical prostatectomy were enrolled in the study. Under general anesthesia, about 50 mL of adipose tissue was obtained from the patients by liposuction. ADRCs were obtained by separation with centrifugation using the Celution cell-processing device. A mixture of ADRCs and adipose tissue were transurethrally injected into the submucosal space of the membranous urethra. Functional and anatomical improvement was assessed using a 24-h pad test, validated patient questionnaire, urethral pressure profile, and magnetic resonance imaging (MRI) during 12-week follow-up. RESULTS: Urine leakage volume was improved with time in all patients in the 24-h pad test, with the exemption of temporal deterioration at the first 2 weeks post-injection in 2 patients. Subjective symptoms and quality of life assessed on the basis of questionnaire results showed similar improvement. The mean maximum urethral closing pressure increased from 44.0 to 63.5 cm H2O at 12 weeks after injection. MRI showed an increase in functional urethral length (from 6.1 to 8.3 mm) between the lower rim of the pubic bone and the bladder neck. Adverse events, such as pelvic pain, inflammation, or de novo urgency, were not observed in any case during follow-up. CONCLUSION: This study demonstrated that transurethral injection of autologous ADRCs can be a safe and effective treatment modality for postprostatectomy incontinence.


Subject(s)
Adipose Tissue/cytology , Aged , Female , Humans , Injections/methods , Magnetic Resonance Imaging , Male , Middle Aged , Prostatectomy/adverse effects , Quality of Life , Stem Cell Transplantation/methods , Surveys and Questionnaires , Transplantation, Autologous , Treatment Outcome , Urethra/diagnostic imaging , Urinary Incontinence/etiology
19.
Acta cir. bras ; 30(2): 100-106, 02/2015. tab, graf
Article in English | LILACS | ID: lil-741021

ABSTRACT

PURPOSE: To evaluate which is the best route of administration for cell therapy in experimental rat model of small-for size syndrome. METHODS: A total of 40 rats underwent partial hepatectomy (70%) that induces the small-for-size syndrome and were divided into four groups of route administration: intravenous, intraperitoneal, enteral and tracheal. The small-for-size syndrome model was designed with extended partial hepatectomy (70%). The animals were divided into four groups of routes administration: intravenous (n=10) - intravenously through the dorsal vein of the penis; intraperitoneal (n=10) - intraperitoneally in the abdominal cavity; enteral (n=10) - oroenteral with the placement of a number 4 urethral probe and maintained at third duodenal portion; tracheal (n=10) - after tracheal intubation. We track the animals and monitor them for 21 days; during this follow-up we evaluated the result of cell therapy application tracking animals using ultrasound, radiography and PET-scan. Statistical analysis was performed using GraphPad Prism Software(r). Differences were considered significant with the p<0.05. Data are presented as the median and variation for continuous variables. Comparisons between groups were made using analysis of the imaging test by the researchers. RESULTS: All four groups underwent partial hepatectomy of 70% liver tissue targeting the same weight of resected liver. Initially the PET-scan tests showed similarity in administered cells by different routes. However, in few days the route of intravenous administration showed to be the most appropriated to lead cells to the liver followed by enteral. The tracheal and peritoneal routes were not as much successful for this goal. CONCLUSION: The intravenous route is the best one to cell therapy in experimental rat model of small-for size-syndrome. .


Subject(s)
Animals , Male , Disease Models, Animal , Drug Administration Routes , Liver Diseases/therapy , Liver Regeneration/physiology , Stem Cell Transplantation/methods , Cell- and Tissue-Based Therapy/methods , Hepatectomy , Liver Transplantation/adverse effects , Liver/chemistry , Organ Size , Rats, Sprague-Dawley , Reproducibility of Results , Syndrome , Time Factors
20.
Rev. Ateneo Argent. Odontol ; 54(2): 31-34, 2015. ilus, tab
Article in Spanish | LILACS | ID: lil-776103

ABSTRACT

En los días actuales, con los avances de la ingeniería de tejidos, el principal objetivo de los investigadores es desarrollar una tercera dentición, utilizando células madre. Nuestro trabajo tuvo como objetivo un levantamiento bibliográfico acerca de la utilización de células madre en odontología para la regeneración de los tejidos bucales. Para este trabajo, llevamos en consideración la información de artículos nacionales e internacionales de 2006 hasta 2014, construyendo una tabla. Sabemos que las células madre son muy especiales y prometen revolucionar la historia de la odontología mundialmente, solucionando grandes problemas clínicos.


Subject(s)
Humans , Stem Cells/physiology , Tissue Engineering/methods , Dental Pulp/physiology , Stem Cell Transplantation/methods , Tissue Scaffolds , Embryonic Stem Cells/physiology , Stem Cell Research/methods
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