Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 2.903
Filter
1.
Braz. dent. j ; 32(3): 65-74, May-June 2021. tab, graf
Article in English | LILACS, BBO | ID: biblio-1345502

ABSTRACT

Abstract This study investigated the effect of three commercial calcium silicate-based materials (CSBM) on cytotoxicity and pro-and anti-inflammatory cytokines production in cultured human periodontal ligament stem cells (hPDLSCs). Culture of hPDLSCs was established and characterized. Extracts of Bio-C Sealer (Angelus, Londrina, PR, Brazil), MTA Fillapex (Angelus, Londrina, PR, Brazil) and PBS Cimmo HP (Cimmo Soluções em Saúde, Pouso Alegre, MG, Brazil) were prepared by placing cement specimens (5 x 3 mm) in culture medium. Then, the extracts were serially two-fold diluted (1, 1:2, 1:4, 1:8, 1:16) and inserted into the cell-seeded wells for 24, 48 and 72 h for MTT assays. TNF-α and IL-10 cytokines were quantified by ELISA at 24h-cell supernatants. Data were analyzed by ANOVA and Tukey's test (α = 0.05). All CSBM exhibited some cytotoxicity that varied according to extract concentration and time of evaluation. MTA Fillapex presented the highest cytotoxic effects with significant reduction of metabolic activity/cell viability when compared to Bio-C Sealer and Cimmo HP®. TNF-α was significantly upregulated by the three tested cements (p < 0.05) while only MTA Fillapex significantly upregulated IL-10 in comparison to control. Taken collectively, the results showed that PBS Cimmo HP®, Bio-C Sealer and MTA Fillapex present mild and transient cytotoxicity and slightly induced TNF-α production. MTA Fillapex upregulated IL-10 release by hPDLSCs.


Resumo Este estudo investigou o efeito de três materiais comerciais à base de silicato de cálcio (CSBM) na citotoxicidade e na produção de citocinas pró e antiinflamatórias em células-tronco do ligamento periodontal humano (hPDLSCs). Cultura de hPDLSCs foi estabelecida e caracterizada. Extratos de Bio-C Sealer (Angelus, Londrina, PR, Brasil), MTA Fillapex (Angelus, Londrina, PR, Brasil) e PBS Cimmo HP® (Cimmo Soluções em Saúde, Pouso Alegre, MG, Brasil) foram preparados com a colocação de espécimes dos cimentos (5 x 3 mm) em meio de cultura. Em seguida, os extratos foram diluídos (1, 1: 2, 1: 4, 1: 8, 1:16) e inseridos nos poços semeados de células para ensaio de citotoxicidade por meio de MTT por 24, 48 e 72 h. As citocinas TNF-α e IL-10 foram quantificadas por ELISA em sobrenadantes de células de 24 h. Os dados foram analisados por ANOVA e teste de Tukey (α = 0,05). Todos os CSBM exibiram alguma citotoxicidade que variou de acordo com a concentração do extrato e o tempo de avaliação. O MTA Fillapex apresentou os maiores efeitos citotóxicos com redução significativa da atividade metabólica / viabilidade celular quando comparado ao Bio-C Sealer e Cimmo HP®. O TNF-α foi regulado positivamente pelos três cimentos testados (p <0,05), enquanto apenas o MTA Fillapex regulou positivamente a liberação de IL-10 em comparação com o controle. Tomados em conjunto, os resultados mostraram que PBS Cimmo HP®, Bio-C Sealer e MTA Fillapex apresentam citotoxicidade leve e transitória e induziram a produção de TNF-α. O MTA Fillapex regulou positivamente a liberação de IL-10 por hPDLSCs.


Subject(s)
Humans , Periodontal Ligament/cytology , Root Canal Filling Materials/adverse effects , Stem Cells/drug effects , Silicates/adverse effects , Calcium Compounds/adverse effects , Oxides , Materials Testing , Cytokines/metabolism , Aluminum Compounds
2.
Arq. bras. oftalmol ; 84(3): 282-296, May-June 2021. tab, graf
Article in English | LILACS | ID: biblio-1248965

ABSTRACT

ABSTRACT This review is intended to describe the therapeutic approaches for corneal blindness, detailing the steps and elements involved in corneal wound healing. It also presents the limitations of the actual surgical and pharmacological strategies used to restore and maintain corneal transparency in terms of long-term survival and geographic coverage. In addition, we critically review the perspectives of anabolic agents, including vitamin A, hormones, growth factors, and novel promitotic and anti-inflammatory modulators, to assist corneal wound healing. We discuss the studies involving nanotechnology, gene therapy, and tissue reengineering as potential future strategies to work solely or in combination with corneal surgery to prevent or revert corneal blindness.(AU)


RESUMO O presente trabalho traz uma revisão das abordagens terapêuticas para a cegueira da córnea. O estudo detalha as etapas e os elementos envolvidos na cicatrização da córnea. Ele mostra as limitações das estratégias cirúrgicas e farmacológicas usadas para restaurar e manter a transparência da córnea em termos de sobrevida a longo prazo e alcance geográfico. As perspectivas dos agentes anabólicos, incluindo vitamina A, hormônios, fatores de crescimento e novos moduladores pró-mitóticos e anti-inflamatórios para auxiliar a cicatrização da ferida na córnea, são revisadas criticamente. Aqui, apresentamos estudos envolvendo nanotecnologia, terapia gênica e reengenharia de tecidos como possíveis estratégias futuras para atuar de maneira isolada ou combinada com a cirurgia da córnea para prevenir ou reverter a cegueira corneana.(AU)


Subject(s)
Humans , Blindness/prevention & control , Blindness/therapy , Corneal Injuries/prevention & control , Corneal Injuries/therapy , Stem Cells , Vitamin A/therapeutic use , Genetic Therapy/instrumentation , Nanotechnology/instrumentation , Intercellular Signaling Peptides and Proteins/therapeutic use , Hormones/therapeutic use , Anti-Inflammatory Agents/therapeutic use
3.
Rev. cienc. salud (Bogotá) ; 19(1): 74-87, ene.-abr. 2021. tab, graf
Article in English | LILACS | ID: biblio-1289167

ABSTRACT

Abstract Introduction: Multipurpose solutions (MPS) for soft contact lenses (SCL) play an essential role in inhibiting potentially pathogenic agents. Their antimicrobial effectiveness is assessed in vitro and their safety in vivo, with clinical trials that include a combination of different solutions and lens materials. The objective is to assess the biocompatibility of a new SCL MPS produced in Colombia that contains polyhexamethylene biguanide (PHMB) and to determine its antimicrobial activity. Materials and Methods: This was a crossover study with 25 subjects who did not wear lens and who were fitted with different combinations of five SCL materials with either MPS or control physiological saline solution (CS). Corneal thickness, conjunctival hyperemia, corneal staining, and comfort were assessed after two hours of wearing SCL. Antimicrobial effectiveness was measured using ISO 14729 standard assays. Results: When considering SCL material, there was a statistically significant difference between the new MPS and the CS for Comfilcon A (p < 0.05). There was no statistical or clinically significant difference for corneal thickness or corneal staining between the combination of lens material and new MPS with the CS (p > 0.05). After two hours of lens insertion, comfort scores were higher than 7.8. The MPS reduced bacteria colony forming units (CFU) in over 3 log, and fungal CFU in over 1.0 log. Conclusions: The new MPS met the antimicrobial standards of ISO 14729, is considered safe and biocompatible with the ocular surface and retains high comfort levels.


Resumen Introducción: las soluciones multipropósito (SMP) para lentes de contacto blandos (LCB) desempeñan un papel esencial en la inhibición de agentes potencialmente patógenos. Su efectividad antimicrobiana se evalúa in vitro, y su seguridad, in vivo, con ensayos clínicos que incluyen una combinación de diferentes soluciones y materiales para lentes. El objetivo es evaluar la biocompatibilidad de una nueva SMP producida en Colombia que contiene polihexametileno biguanida (PHMB) y determinar su actividad antimicrobiana. Materiales y métodos: estudio cruzado con 25 sujetos no usuarios de lentes, que fueron adaptados con cinco combinaciones diferentes de materiales de LCB con una nueva SMP o solución salina fisiológica de control (CS). El grosor corneal, la hiperemia conjuntival, la tinción corneal y la comodidad se evaluaron después de dos horas de uso del LC. La efectividad antimicrobiana se midió utilizando ensayos estándar ISO 14729. Resultados: considerando el material del LCB, solo hubo una diferencia estadísticamente significativa entre la nueva SMP y el CS para el Comfilcon A (p < 0.05). Tampoco hubo diferencias estadísticamente o clínicamente significativas para el grosor corneal o la tinción corneal, entre la combinación del material del lente y la nueva SMP con el CS (p > 0.05). Después de dos horas de uso del lente, las puntuaciones de confort fueron superiores a 7.8. La SMP redujo las unidades formadoras de colonias (UFC) de bacterias en más de 3 log, y las UFC fúngicas en más de 1.0 log. Conclusiones: la nueva SMP cumplió con los estándares antimicrobianos de ISO 14729, y se considera segura y biocompatible con la superficie ocular, con altos niveles de confort.


Resumo Introdução: as soluções multipropósito (SMP) para lentes de contato macias (LCM) apresentam um papel essencial na inibição de agentes potencialmente patógenos. Sua eficácia como agente antimicrobiano se valia in vitro, e sua segurança, in vivo, como ensaios clínicos que incluem uma combinação de diferentes soluções e materiais para lentes. O objetivo é avaliar a biocompatibilidade de uma nova SMP produzida na Colômbia a base de polihexametileno biguanida (PHMB) e determinar seu potencial antimicrobiano. Materiais e métodos: estudo cruzado com 25 indivíduos não usuários de lentes, que foram adaptados com cinco combinações diferentes de LCM como uma nova SMP ou solução salina fisiológica como controle (CS). A espessura da córnea, a hiperemia conjuntival, a coloração da córnea e a comodidade, foram avaliadas após duas horas de uso da LCB. A eficácia antimicrobiana foi medida com ensaios padrão ISO 14729. Resultados: considerando o material da LCB, houve apenas uma diferença estatisticamente significativa entre a nova SMP e o CS, paro o Comfilcon A (p <0.05). Não houve diferença estatisticamente ou clinicamente significativa para a espessura da córnea ou a coloração da córnea, entre a combinação do material da lente e a nova SMP com o controle CS (p > 0.05). Após duas horas de uso, as pontuações de conforto foram superiores a 7,8. A SMP reduziu as unidades formadoras de colônias (UFC) de bactérias em mais de 3 log, e as UFC fúngicas em mais de 1.0 log. Conclusões: a nova SMP cumpriu com os padrões antimicrobianos ISO 14729, é considerada segura e biocompatível com a superfície ocular, com altos níveis de conforto.


Subject(s)
Humans , Contact Lenses, Hydrophilic , Hyperemia , Stem Cells
4.
Medisan ; 25(2)mar.-abr. 2021.
Article in Spanish | LILACS, CUMED | ID: biblio-1250353

ABSTRACT

La endodoncia regenerativa comprende un conjunto de procedimientos biológicos que se efectúan en los dientes permanentes con ápice no formado y necrosis pulpar, cuyo fin es sustituir los tejidos dañados, incluidas la dentina y la estructura radicular, así como las células del complejo pulpodentinario. En este artículo se describen las bases celulares y moleculares de esta terapia, sustentada en una compleja interacción entre las células madre dentales de la papila apical, los factores de crecimiento y los biomateriales con el microambiente donde se va a restablecer. Asimismo, se argumenta cómo las condiciones ambientales en las que se lleva a cabo el proceso terapéutico influyen en la regeneración, con una función esencial en la regulación de la diferenciación de los tejidos.


The regenerative endodontics comprises a group of biological procedures that are made in the permanent teeth with non-formed apex and pulpar necrosis whose end is to substitute the damaged tissues, including the dentine and radicular structure, as well as the cells of the dentin pulp complex. The cellular and molecular basis of this therapy are described in this work, sustained in a complex interaction between the dental stem cells of the apical papilla, the growth factors and biomaterials with the microenvironment where it will be recover. Likewise, it was argued how the environmental conditions in which the therapeutic process is carried out influence in the regeneration, with an essential function in the regulation of the differentiation of tissues.


Subject(s)
Stem Cells , Dentition, Permanent , Regenerative Endodontics/methods , Biocompatible Materials
5.
Rev. cuba. hematol. inmunol. hemoter ; 37(1): e1310, ene.-mar. 2021.
Article in Spanish | LILACS, CUMED | ID: biblio-1251720

ABSTRACT

Introducción: Los síndromes mielodisplásicos constituyen un grupo heterogéneo de desórdenes hematológicos clonales adquiridos, que afectan la célula madre. Se caracterizan morfológicamente por: hematopoyesis ineficaz, citopenias periféricas progresivas, displasia en uno o más linajes celulares y tendencia evolutiva a leucemia aguda. Los avances recientes en la comprensión de los mecanismos genéticos y moleculares de los síndromes mielodisplásicos, han revelado la asociación entre alteraciones inmunológicas y las mutaciones recurrentes. Las células de la respuesta inmune innata y adaptativa, así como diversos mediadores solubles liberados por ellas, pueden establecer una respuesta antitumoral protectora o, por el contrario, inducir eventos de inflamación crónica que favorezcan la promoción y progresión de esta enfermedad. Objetivos: Resumir los conocimientos actuales de la relación sistema inmune-síndromes mielodisplásicos, enfatizando en las células inmunes del microambiente de la médula ósea y su importancia en la clínica de la enfermedad. Métodos: Se realizó investigación bibliográfica-documental acerca del tema. Se consultaron las bases de datos Scielo y Pubmed. Conclusiones: La comprensión de la función dual que ejerce el sistema inmune en los síndromes mielodisplásicos, constituye un desafío y son necesarios estudios clínicos rigurosos para poder establecer el valor de la manipulación del sistema inmune como una forma posible de tratamiento de esta enfermedad(AU)


Introduction: Myelodysplastic syndromes (MDS) constitute a heterogeneous group of acquired clonal hematological disorders that affect the stem cell. These are characterized morphologically and clinically by: ineffective hematopoiesis, progressive peripheral cytopenia, dysplasia in one or more cell lineages, in most of cases and evolutionary tendency to acute leukemia. Recent advances in understanding the genetic and molecular mechanisms of MDS have revealed the association between immunological alterations and recurrent mutations. Cells of the innate and adaptive immune response, as well as various soluble mediators released by them, can establish a protective antitumor response or, on the contrary, induce events of chronic inflammation that favor the promotion and progression of this disease. Objective: To summarize the current knowledge of the immune system-MDS relationship, emphasizing the immune cells of the bone marrow microenvironment and their importance in the clinic of the disease. Methods: A bibliographic-documentary research was carried out on the subject. The Scielo and Pubmed databases were consulted. Conclusions: Understanding the dual role of the immune system in MDS constitutes a challenge and rigorous clinical studies are necessary to establish the value of manipulating the immune system as a possible form of treatment of this disease(AU)


Subject(s)
Humans , Stem Cells , Myelodysplastic Syndromes/complications , Leukemia , Adaptive Immunity , Hematopoiesis/genetics , Immune System/physiopathology , Inflammation/diagnosis
6.
Article in Chinese | WPRIM | ID: wpr-880087

ABSTRACT

OBJECTIVE@#To investigate the effect of etoposide (ETO) on elimination of chronic myeloid leukemia (CML) stem cells by imatinib mesylate(IM) in vivo.@*METHODS@#SCL-tTA/BCR-ABL mice were used as CML animal model. Flow cytometry was used to assess the effect of ETO alone or in combination with IM on the number of leukemia stem cell (LSC) in bone marrow and spleen, and peripheral blood neutrophils in CML mice and normal control FVB mice.@*RESULTS@#The results showed that in CML mice, the number and proportion of LSC in bone marrow and the proportion of neutrophils in peripheral blood decreased significantly after ETO and IM combined treatment, and the degree of decrease was more significant than that of both alone. While in wild type FVB mice, the combination of ETO and IM showed no significant effect on the number and proportion of LSK cells in bone marrow and the proportion of neutrophils in spleen.@*CONCLUSION@#ETO can selectively enhance elimination of CML LSC by IM in vivo.


Subject(s)
Animals , Drug Resistance, Neoplasm , Etoposide , Fusion Proteins, bcr-abl , Imatinib Mesylate , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/drug therapy , Mice , Stem Cells
7.
Article in English | WPRIM | ID: wpr-878418

ABSTRACT

Cartilage stem cells (CSCs) are cells that self-proliferate, have surface antigen expression, and have multidirectional differentiation potential in the articular cartilage. CSCs, as an ideal source of stem cells, has a good application prospect in stem cell therapy. This article reviews the CSCs markers, cartilage differentiation signaling pathway, and clinical treatment of osteoarthritis.


Subject(s)
Cartilage, Articular , Cell Differentiation , Chondrocytes , Humans , Osteoarthritis , Stem Cells
8.
Acta Physiologica Sinica ; (6): 181-196, 2021.
Article in English | WPRIM | ID: wpr-878247

ABSTRACT

Organoids are self-organized cellular clusters in three-dimensional culture, which can be derived from a single stem cell, progenitor or cell clusters of different lineages resembling in vivo tissue architecture of an organ. In the recent years, organoids technology has contributed to the revolutionary changes in stem cell and cancer fields. In this review, we have briefly overviewed the emerging landscape of prostate organoid technology (POT) in prostate research. In addition, we have also summarized the potential application of POT in the understanding of prostate stem cell and cancer biology and the discovery of novel therapeutic strategies for prostate cancer. Lastly, we have critically discussed key challenges that lie in the current state of POT and provided a future perspective on the second-generation of POT, which should better recapitulate cellular behaviors and drug responses of prostate cancer patients.


Subject(s)
Gold , Humans , Male , Neoplasms , Organoids , Prostate , Stem Cells , Technology
9.
J. appl. oral sci ; 29: e20210138, 2021. tab, graf
Article in English | LILACS | ID: biblio-1340112

ABSTRACT

Abstract Mesenchymal and epithelial stem cells were identified in dental tissues; however, knowledge about the odontogenic stem cells is limited, and there are some questions regarding their temporo-spatial dynamics in tooth development. Objective Our study aimed to analyze the expression of the stem cell markers CD146 and p75NTR during the different stages of odontogenesis. Methodology The groups consisted of 13.5, 15.5, 17.5 days old embryos, and 14 days postnatal BALB/c mice. The expression of CD146 and p75NTR was evaluated by immunohistochemistry. Results Our results showed that positive cells for both markers were present in all stages of tooth development, and the number of positive cells increased with the progression of this process. Cells of epithelial and ectomesenchymal origin were positive for CD146, and the expression of p75NTR was mainly detected in the dental papilla and dental follicle. In the postnatal group, dental pulp cells were positive for CD146, and the reduced enamel epithelium and the oral mucosa epithelium showed immunostaining for p75NTR. Conclusions These results suggest that the staining pattern of CD146 and p75NTR underwent temporal and spatial changes during odontogenesis and both markers were expressed by epithelial and mesenchymal cell types, which is relevant due to the significance of the epithelial-ectomesenchymal interactions in tooth development.


Subject(s)
Animals , Mice , Mesenchymal Stem Cells , Odontogenesis , Stem Cells , Cell Differentiation , Receptors, Nerve Growth Factor , CD146 Antigen , Mice, Inbred BALB C
10.
J. appl. oral sci ; 29: e20210296, 2021. graf
Article in English | LILACS | ID: biblio-1340101

ABSTRACT

Abstract Objectives Human dental pulp stem cells (DPSCs) have been used to regenerate damaged nervous tissues. However, the methods of committing DPSCs into neural stem/progenitor cells (NSPCs) or neurospheres are highly diverse, resulting in many neuronal differentiation outcomes. This study aims to validate an optimal protocol for inducing DPSCs into neurospheres and neurons. Methodology After isolation and characterization of mesenchymal stem cell identity, DPSCs were cultured in a NSPC induction medium and culture vessels. The durations of the culture, dissociation methods, and passage numbers of DPSCs were varied. Results Neurosphere formation requires a special surface that inhibits cell attachment. Five-days was the most appropriate duration for generating proliferative neurospheres and they strongly expressed Nestin, an NSPC marker. Neurosphere reformation after being dissociated by the Accutase enzyme was significantly higher than other methods. Passage number of DPSCs did not affect neurosphere formation, but did influence neuronal differentiation. We found that the cells expressing a neuronal marker, β-tubulin III, and exhibiting neuronal morphology were significantly higher in the early passage of the DPSCs. Conclusion These results suggest a guideline to obtain a high efficiency of neurospheres and neuronal differentiation from DPSCs for further study and neurodegeneration therapeutics.


Subject(s)
Humans , Stem Cells , Dental Pulp , Cell Differentiation
11.
Pesqui. vet. bras ; 40(12): 1018-1028, Dec. 2020. tab, graf, ilus
Article in English | ID: biblio-1155043

ABSTRACT

The present study aimed to evaluate the effects of mesenchymal stem cells derived from canine adipose tissue in the healing process of full-thickness mesh skin grafts in rabbits. The stem cells were collected from young dogs; and, after characterization, remained in cryopreservation, in independent doses containing 2 x 106 cells. The mesh distal limb graft technique was performed in 60 rabbits, divided into three groups, CG (Control Group), GT1 (Intralesional Stem Cell Treated Group), and GT2 (Intravenous Stem Cell Treated Group), containing 20 animals each. After grafting, each group was randomly divided into four subgroups according to euthanasia time 3, 7, 14, and 30 days, containing five animals in each group. Animals of GT1_14, GT1_30, and GT2_14, GT2_30 subgroups received a second dose of xenogeneic cells on the seventh day. Meanwhile, animals from GT1_30 and GT2_30 received the third dose of xenogeneic cells on day 14. The groups treated with xenogeneic stem cells positively affected type III collagen re-epithelialization and deposition, and possibly GT1 had a controlled inflammatory response. However, no effect on angiogenesis. Thus, it was possible to demonstrate tolerance and therapeutic action of mesenchymal stem cells from canine adipose tissue in skin grafts in rabbits.(AU)


O presente estudo teve como principal objetivo avaliar os efeitos das células-tronco mesenquimais derivadas do tecido adiposo de cães no processo de cicatrização de autoenxertos de pele de espessura total em malha em coelhos. As células-tronco foram coletadas de cães jovens, após a caracterização estas permaneceram em criopreservação, em doses individuais contendo 2 x 106 células. A técnica de enxerto em malha na região distal do membro foi realizada em 60 coelhos, divididos em três grupos, GC (Grupo Controle), GT1 (Grupo tratado com células-tronco intralesional) e GT2 (Grupo tratado com células-tronco via endovenosa), contendo 20 animais cada. Imediatamente após a enxertia, cada grupo foi dividido aleatoriamente em quatro subgrupos, de acordo com o tempo de eutanásia 3, 7, 14 e 30 dias contendo cinco animais cada. Animais dos subgrupos GT1_14, GT1_30 e GT2_14, GT2_30 receberam uma segunda dose de células xenógenas no sétimo dia. Ademais, animais do GT1_30 e do GT2_30 receberam a terceira dose de células xenógenas no dia 14. Os grupos tratados com células-tronco xenógenas tiveram um efeito positivo na reepitelização e deposição de colágeno tipo III, e possivelmente, o GT1 teve uma resposta inflamatória controlada, entretanto o efeito na angiogênese não foi observado. Dessa forma, foi possível demonstrar que houve tolerância e ação terapêutica das células-tronco mesenquimais derivadas do tecido adiposo de cães em enxertos de pele em coelhos.(AU)


Subject(s)
Animals , Dogs , Rabbits , Stem Cells , Adipose Tissue , Transplants , Mesenchymal Stem Cells , Autografts , Wound Healing , Neovascularization, Physiologic
12.
Int. j. morphol ; 38(6): 1742-1750, Dec. 2020. tab, graf
Article in English | LILACS | ID: biblio-1134507

ABSTRACT

SUMMARY: Mesenchymal stem cells are present in adult tissues such as the human dental pulp. They are pluripotent and can differentiate into various specialized cell types in vitro through appropriate stimuli. Ameloblasts produce human tooth enamel only during embryonic development before tooth eruption, so endogenous regeneration is not possible. Various efforts have been aimed at generating natural or artificial substitutes for dental enamel with properties similar to the specific components of said tissue. The purpose of this study was to induce human dental pulp stem cells to produce enamel proteins using extracellular matrix derived from the rat tail tendon and pigskin. Primary cultures of human dental pulp stem cells were established and characterized by RT-PCR and immunofluorescence, using mesenchymal cell markers such as CD14, CD40, CD44, CD105, and STRO-1. The cells were then incubated with the extracellular matrix for fourteen days and labeled with specific antibodies to detect the expression of dental enamel proteins such as amelogenin, ameloblastin, enamelisin, tuftelin, and parvalbumin, characteristics of the phenotype of ameloblasts. This work demonstrated a positive effect of the extracellular matrix to induce the expression of enamel proteins in the stem cells of the human dental pulp.


RESUMEN: Las células madre mesenquimales están presentes en los tejidos adultos como la pulpa dental humana. Son pluripotentes y pueden diferenciarse en varios tipos de células especializadas in vitro a través de estímulos adecuados. Los ameloblastos producen esmalte dental humano sólo durante el desarrollo embrionario antes de la erupción dental, por lo que no es posible su regeneración endógena. Varios esfuerzos se han orientado a generar sustitutos naturales o artificiales de esmalte dental con propiedades similares a los componentes específicos de este tejido. El propósito de este estudio fue inducir células madre de pulpa dental humana para producir proteínas del esmalte dental a través del estímulo de matriz extracelular derivada del tendón de la cola de rata y piel de cerdo. Se establecieron cultivos primarios de células madre de pulpa dental humana y se caracterizaron por RT-PCR e inmunofluorescencia utilizando marcadores de células mesenquimales como CD14, CD40, CD44, CD105 y STRO-1. Posteriormente, las células se incubaron con matriz extracelular durante un período de catorce días y se marcaron con anticuerpos específicos para detectar la expresión de proteínas de esmalte dental como amelogenina, ameloblastina, enamelisina, tuftelina y parvalbúmina, las cuales son características del fenotipo de ameloblastos. Este trabajo demostró el efecto positivo que tiene el empleo de la matriz extracelular para inducir la expresión de proteínas de esmalte en las células pluripotenciales de la pulpa dental humana.


Subject(s)
Humans , Stem Cells , Dental Enamel Proteins , Dental Pulp , Extracellular Matrix , Immunophenotyping , Fluorescent Antibody Technique , Cell Culture Techniques , Tissue Engineering
13.
J. oral res. (Impresa) ; 9(6): 522-531, dic. 31, 2020. ilus, tab
Article in English | LILACS | ID: biblio-1178951

ABSTRACT

Three-dimensional (3D) bioprinting of cells is an emerging area of research but has not been explored yet in the context of periodontal tissue engineering. Objetive: This study reports on the optimization of the 3D bioprinting scaffolds and tissues used that could be applied clinically to seniors for the regenerative purpose to meet individual patient treatment needs. Material and Methods: We methodically explored the printability of various tissues (dentin pulp stem/progenitor cells, periodontal ligament stem/progenitor cells, alveolar bone stem/progenitor cells, advanced platelet-rich fibrin and injected platelet-rich fibrin) and scaffolds using 3D printers pertaining only to periodontal defects. The influence of different printing parameters with the help of scaffold to promote periodontal regeneration and to replace the lost structure has been evaluated. Results: This systematic evaluation enabled the selection of the most suited printing conditions for achieving high printing resolution, dimensional stability, and cell viability for 3D bioprinting of periodontal ligament cells. Conclusion: The optimized bioprinting system is the first step towards the reproducible manufacturing of cell laden, space maintaining scaffolds for the treatment of periodontal lesions.


La bioimpresión tridimensional (3D) de células es un área emergente de investigación, pero aún no se ha explorado en el contexto de la ingeniería de tejidos periodontales. Objetivo: Este estudio informa sobre la optimización de los tejidos y andamios de bioimpresión 3D utilizados que podrían aplicarse a personas mayores en el entorno clínico con fines regenerativos para satisfacer las necesidades de tratamiento de cada paciente. Material y Métodos: Exploramos metódicamente la capacidad de impresión de varios tejidos (células madre / progenitoras de la pulpa de dentina, células madre / progenitoras del ligamento periodontal, células madre / progenitoras de hueso alveolar, fibrina rica en plaquetas avanzada y fibrina rica en plaquetas inyectada) y andamios utilizando impresoras 3D que pertenecen solo a defectos periodontales. Se ha evaluado la influencia de diferentes parámetros de impresión con la ayuda de andamios para promover la regeneración periodontal y reemplazar la estructura perdida. Resultados: Esta evaluación sistemática permitió la selección de las condiciones de impresión más adecuadas para lograr una alta resolución de impresión, estabilidad dimensional y viabilidad celular para la bioimpresión 3D de células del ligamento periodontal. Conclusión: El sistema de bioimpresión optimizado es el primer paso hacia la fabricación reproducible de andamios de mantenimiento de espacio cargados de células para el tratamiento de lesiones periodontales


Subject(s)
Humans , Tissue Engineering/methods , Bioprinting/methods , Printing, Three-Dimensional , Periodontal Diseases/therapy , Regeneration , Stem Cells
14.
Int. j. morphol ; 38(5): 1463-1472, oct. 2020. graf
Article in English | LILACS | ID: biblio-1134463

ABSTRACT

SUMMARY: The vomeronasal organ (VNO) is an accessory organ involved on the olfactory pathway, that detects pheromones and emits signals in order to modulate social and reproductive behavior. The VNO stem cells replace neurons throughout life. The aim of this study was to isolate and characterize cells derived from the vomeronasal organ from New Zealand rabbits. Five male rabbits with 120 days were used for cell isolation and culture. Results: VNO-derived cells presented labelling for proliferation (PCNA), undifferentiated profile (Nanog), neuronal (GFAP), mesenchymal stem cells (CD73, CD90 and CD105 and Stro-1). Also, presence of cytoskeletal (Vimentin, b-tubulin and CK-18) and absence of hematopoietic markers (CD34, CD117 and CD45) both by immunofluorescence and flow cytometry. By PCR it was possible to verify the expression of some undifferentiated profile (Oct-4), neuronal (Nestin) and mesenchymal (CD73, CD105 and Vimentin) genes. Functionally, VNO-derived cells differentiate in vitro into adipocytes, osteocytes and chondrocytes, and presented no tumorigenic potential when injected to Balb/c nu/nu mice. In conclusion, the rabbit VNO-derived cells have a profile that could be supportive to VNO olfactory/neuroreceptor epithelium by delivering factors to epithelial turnover or even by differentiation into epithelial cells to replacement of commissural epithelium.


RESUMEN: El órgano vomeronasal (OVN) es un órgano accesorio de la vía olfatoria, que detecta feromonas y emite señales que afectan la modulación del comportamiento social y reproductivo. Las células madre OVN reemplazan las neuronas durante toda la vida. El objetivo de este estudio fue aislar y caracterizar células derivadas del órgano vomeronasal de conejos raza Nueva Zelanda. Para el aislamiento y el cultivo celular se utilizaron cinco conejos machos con una edad de 120 días. Las células del OVN presentaron etiquetado para la proliferación (PCNA), un perfil indiferenciado (Nanog), neuronal (GFAP), células madre mesenquimales (CD73, CD90 y CD105 y Stro-1). Además, se ob- servó presencia de citoesqueleto (Vimentina, β-tubulina y CK-18) y ausencia de marcadores hematopoyéticos (CD34, CD117 y CD45) tanto por inmunofluorescencia como por citometría de flujo. Me- diante PCR fue posible verificar la expresión de algunos genes de perfil indiferenciado (Oct-4), neuronal (Nestin) y mesenquimatoso (CD73, CD105 y Vimentin). Las células derivadas del OVN se diferencian in vitro en adipocitos, osteocitos y condrocitos, y no presentan un potencial tumorigénico al ser infiltrados en ratones Balb / c nu / nu. En conclusión, las células derivadas de OVN de conejo tienen un perfil que podría ser compatible con el epitelio olfatorio / neurorreceptor de OVN transmitiendo factores al recambio epitelial o incluso mediante la diferenciación en células epiteliales para reemplazar el epitelio comisural.


Subject(s)
Animals , Rabbits/anatomy & histology , Vomeronasal Organ/cytology , Mesenchymal Stem Cells/physiology , Olfactory Bulb/cytology , Stem Cells/physiology , Olfactory Mucosa/cytology , Polymerase Chain Reaction , Fluorescent Antibody Technique , Flow Cytometry , Neurons/physiology
15.
Rev. colomb. cardiol ; 27(4): 294-302, jul.-ago. 2020. tab, graf
Article in Spanish | LILACS, COLNAL | ID: biblio-1289228

ABSTRACT

Resumen Objetivo: describir el estado del arte del marcapasos biológico y las perspectivas para crear tejido cardíaco de marcapasos utilizando modernas tecnologías genéticas y de ingeniería de tejidos. Métodos: revisión sistemática de la literatura. Resultados: los marcapasos se han convertido en el tratamiento primordial para cierto tipo de arritmias o bloqueos avanzados sintomáticos. Somos testigos de mejoras continuas en la tecnología del dispositivo, con avances en el diseño del cable, el tamaño del generador, la longevidad de la batería y los algoritmos de software que se han traducido en dispositivos más pequeños con funcionalidad mejorada. En la actualidad existen muchos sistemas implantables de cardioestimulación capaces de reemplazar la función de los marcapasos fisiológicos (seno y nódulos aurículo-ventriculares) que incluyen los recientemente desarrollados marcapasos secuenciales y autoprogramables. En la última década la investigación ha confirmado que el marcapasos biológico se puede crear mediante la terapia génica y la terapia celular. Hoy existen dos enfoques para construir marcapasos biológicos: uno es para introducir genes de marcapasos en células madre mesenquimales, y el otro es para inducir células madre pluripotentes en las células del nódulo sinoauricular. Conclusiones: los marcapasos biológicos, actualmente en la etapa preclínica, podrían ser una alternativa a los dispositivos electrónicos para pacientes seleccionados en el futuro.


Abstract Objective: To describe the state of the art of biological pacemakers and the perspectives for creating cardiac pacing tissue using modern genetic and tissue engineering technologies. Methods: A systematic review of the literature. Results: Pacemakers have become the first line treatment for certain types of arrhythmias and advanced symptomatic blocks. We are witnessing continuous improvements in the technology of the device, with advances in the design of the cable, the size of the generator, the longevity of the battery, as well as the software algorithms that have led to smaller devices with improved functions. There are currently many cardiac stimulation implantable systems capable of replacing the function of physiological pacemakers systems (sinus and atrial-ventricular nodes) that include the recently developed sequential and self-programmable pacemakers. In the last ten years or so, studies have confirmed that biological pacemakers can be created using gene therapy and cell therapy. There are currently to main efforts to construct biological pacemakers. One is to introduce pacemaker genes in mesenchymal stem cells, and the other is to introduce pluripotent stem cells in cells of the sinoatrial node. Conclusions: Biological pacemakers, currently in the pre-clinical stage, could be an alternative to the electronic devices for selected patients in the future.


Subject(s)
Humans , Pacemaker, Artificial , Stem Cells , Cell- and Tissue-Based Therapy , Genetic Therapy , Tissue Engineering
16.
Rev. cuba. ortop. traumatol ; 34(1): e271, ene.-jun. 2020.
Article in Spanish | LILACS, CUMED | ID: biblio-1139109

ABSTRACT

RESUMEN Objetivos: Describir el efecto terapéutico del uso de células madre en el manejo de la osteonecrosis de la cabeza femoral. Métodos: Se realizó una búsqueda en las distintas bases de datos como Pubmed/MEDLINE, BVS y Cochrane de los últimos 10 años, incluyéndose estudios relevantes de metaanálisis, ensayos clínicos y revisiones. Además, se revisó la página electrónica oficial de la Organización Mundial de la Salud para referencias adicionales. Las palabras de búsqueda fueron: "("Stem Cells" OR "Stem Cell Transplantation"[Mesh] OR "Mesenchymal Stem Cell Transplantation"[Mesh] OR Hematopoietic Stem Cell Transplantation"[Mesh] OR Cell-and Tissue-Based Therapy"[Mesh] "Multipotent Stem Cells"[Mesh] OR "Embryonic Stem Cells"[Mesh]) AND ("Osteonecrosis"[Mesh] OR "Femur Head Necrosis"[Mesh] "osteonecrosis of the femoral head" OR "Femoral head"). Incluimos un total de 7 estudios. 2 metaanálisis, 2 artículos de revisión, 2 ensayos clínicos controlados y aleatorizados y un estudio de evidencia. Resultados: Todos los estudios evidenciaron que la terapia con células madre en el manejo de la osteonecrosis de la cabeza femoral es segura y efectiva. El tratamiento combinado de descompresión central más implantación de células madre mostró mayor efectividad. Conclusiones: La terapia con células madre es una opción terapéutica para tratar la osteonecrosis de la cabeza femoral, sobre todo en etapas tempranas. La combinación de descompresión central más la infiltración de células madre en la zona necrótica produce mejoría de la sintomatología y contiene el progreso de la enfermedad(AU)


ABSTRACT Objectives: To describe the therapeutic effect of the stem cell use in managing osteonecrosis of the femoral head. Methods: A search was carried out in the different databases as Pubmed / MEDLINE, BVS and Cochrane for the last 10 years, including relevant meta-analysis studies, clinical trials and reviews. In addition, the official website of the World Health Organization was checked for additional references. The search words were: "(" Stem Cells "OR" Stem Cell Transplantation "[Mesh] OR" Mesenchymal Stem Cell Transplantation "[Mesh] OR Hematopoietic Stem Cell Transplantation" [Mesh] OR Cell-and Tissue-Based Therapy "[ Mesh] "Multipotent Stem Cells" [Mesh] OR "Embryonic Stem Cells" [Mesh]) AND ("Osteonecrosis" [Mesh] OR "Femur Head Necrosis" [Mesh] "osteonecrosis of the femoral head" OR "Femoral head"). We include a total of 7 studies, 2 meta-analyzes, 2 review articles, 2 controlled and randomized clinical trials, and one evidence study. Findings: All the studies showed that stem cell therapy in managing osteonecrosis of the femoral head is safe and effective. The combined treatment of central decompression plus stem cell implantation showed greater effectiveness. Conclusions: Stem cell therapy is a therapeutic option to treat osteonecrosis of the femoral head, especially in the early stages. The combination of central decompression plus the infiltration of stem cells in the necrotic area produces an improvement in the symptoms and contains the progress of the disease(AU)


Subject(s)
Humans , Stem Cells , Decompression, Surgical/methods , Femur Head Necrosis/therapy
17.
Dental press j. orthod. (Impr.) ; 25(3): 85-92, May-June 2020. graf
Article in English | LILACS, BBO | ID: biblio-1133663

ABSTRACT

ABSTRACT Introduction: Stem cells obtained from the pulp of human deciduous teeth are highly proliferative and plastic multipotent cells, which makes them a relevant model of stem cells, applied in several biomedical areas, with different purposes. Objective: Based on a brief review of the literature, the present work intends to present from conceptual aspects about stem cells, classifications, potential (in vitro and in vivo) applications in dental practice, cell culture, cryopreservation and its importance, ethical and regulatory aspects, as well as the role of the dental surgeon as the endorser responsible for the entire clinical stage that involves the process of collecting stem cells obtained from dental pulps for cryopreservation, with a view to using them under appropriate conditions, in accordance with scientifically proven and justified good laboratory and clinical practices.


RESUMO Introdução: As células-tronco obtidas a partir da polpa de dentes decíduos humanos são células multipotentes altamente proliferativas e plásticas, o que as torna um modelo relevante de células-tronco, aplicado em diversas áreas biomédicas, com diferentes propósitos. Objetivo: A partir de uma breve revisão da literatura, o presente trabalho pretende apresentar desde aspectos conceituais acerca das células-tronco, classificações, potenciais aplicações (in vitro e in vivo) na prática odontológica, cultivo celular, criopreservação e sua importância, aspectos éticos e regulatórios, bem como o papel do cirurgião-dentista como homologador responsável por toda a etapa clínica que envolve o processo de coleta das células-tronco obtidas a partir de polpas dentais para criopreservação, com vistas ao uso em condições adequadas, em acordo com as boas práticas laboratoriais e clínicas cientificamente comprovadas e justificadas.


Subject(s)
Humans , Adult , Mesenchymal Stem Cells , Stem Cells , Tooth, Deciduous , Dental Pulp , Dentistry
18.
Rev. cient. odontol ; 8(1): e008, ene.-abr. 2020.
Article in Spanish | LILACS, LIPECS | ID: biblio-1095501

ABSTRACT

Los restos epiteliales de Malassez son células que se encuentran alrededor de las raíces de las piezas dentarias y forman parte de los tejidos del ligamento periodontal, donde se disponen en forma de red. Las funciones que desempeñan no son muy específicas; sin embargo, se demostró su participación en el mantenimiento del espacio del ligamento periodontal, la regeneración del tejido periodontal, la regeneración del cemento, entre otros. Además, tienen capacidad de diferenciarse en otros linajes celulares, lo que demuestra su capacidad como células madres, y el papel más conocido que tienen es su participación y proliferación en la formación de diversas patologías, como quistes y tumores odontogénicos. (AU)


Epithelial rests of Malassez are cells that are arranged in a network located around the roots of the teeth forming part of the periodontal ligament tissues. The functions that these cells perform are not very specific, however, they have shown to participate in the maintenance of the periodontal ligament space, regeneration of periodontal tissue, including cement regeneration among others. In addition, they are able to differentiate into other cell lineages, thereby demonstrating their capacity as stem cells. These cells are best known for their role in the participation and proliferation of the formation of different pathologies, such as cysts and odontogenic tumors. (AU)


Subject(s)
Humans , Periodontal Ligament , Stem Cells , Odontogenic Cysts , Odontogenic Tumors , Malassezia
19.
Rev. Psicol., Divers. Saúde ; 9(1): 116-123, Março 2020.
Article in English, Portuguese | LILACS | ID: biblio-1254763

ABSTRACT

O artigo visa abordar uma análise, em formato de ensaio analítico a respeito das práticas de guarda de célulastronco. A chamada medicina personalizada e regenerativa emerge ligada ao capitalismo neoliberal e a uma modalidade de governo das condutas baseada na biomedicina e em uma política de gestão da vida focada no mercado neoliberal securitário. A saúde foi transformada em um comércio de células e sustentada nas táticas da bioeconomia, do biovalor e das biotecnologias, articuladas em um dispositivo de governamentalidade reguladora do ser saudável, a partir da compra e venda de materiais biomoleculares e genéticos. Concluindo, buscase interrogar o modo de organizar e funcionar dos biobancos públicos e privados de células-tronco do cordão umbilical e os efeitos destes mecanismos biopolíticos.


The article aims to approach an analysis, in the form of an analytical essay regarding the practices of guarding stem cells. The so-called personalized and regenerative medicine emerges linked to neoliberal capitalism and to a modality of conduct based on biomedicine and a life management policy focused on the neoliberal insurance market. Health was transformed into a trade in cells and sustained by the tactics of bioeconomics, biovalue and biotechnologies, articulated in a governmental device that regulates healthy being, based on the purchase and sale of biomolecular and genetic materials. In conclusion, we seek to question the way of organizing and functioning of public and private umbilical cord stem cell banks and the effects of these biopolitical mechanisms.


Subject(s)
Stem Cells , Regenerative Medicine , Government
20.
Infectio ; 24(1): 50-53, ene.-mar. 2020. graf
Article in Spanish | LILACS, COLNAL | ID: biblio-1090543

ABSTRACT

Resumen Se presenta el caso de un paciente de 20 días de nacido, procedente de Cartagena (Bolívar), hospitalizado por presentar fiebre de 6 días de evolución asociado a sintomatología respiratoria con evaluación neurológica normal. La ecografía obstétrica evidenció una microcefalia con un percentil de perímetro cefálico <2, con hipoplasia del cuerpo calloso y tomografía axial computarizada de cráneo que reportó diámetros cefálicos disminuidos, finas calcificaciones residuales en región frontal-parietal y cambios atróficos cerebrales subcorticales. Se le inició terapia antibiótica por presentar sepsis neonatal, las pruebas serológicas y la PCR para Zika resultaron positivas. Se decidió dar el alta médica al 6 día por mejoría clínica y no presentar déficit neurológico aparente. Aunque no existe un tratamiento específico, el pilar del manejo de un recién nacido con microcefalia es el seguimiento y la vigilancia futura de las posibles comorbilidades, como epilepsia, parálisis cerebral o retraso cognitivo y motor.


Abstract We present the case of a 20-day-old patient from Cartagena (Bolívar), hospitalized for presenting a 6-day fever associated with respiratory symptoms with normal neurological evaluation. The obstetric ultrasound showed a microcephaly with a percentile of cephalic perimeter <2, with hypoplasia of the corpus callosum and computed tomography of the skull that reported decreased cephalic diameters, fine residual calcifications in the frontal-parietal region and atrophic subcortical cerebral changes. Antibiotic therapy was initiated due to neonatal sepsis, the serological tests and the PCR for Zika were positive. It was decided to discharge the hospital after 6 days due to clinical improvement and for not presenting apparent neurological deficit. Although there is no specific treatment, the pillar of the management of a newborn with microcephaly is the monitoring and future surveillance of possible comorbidities, such as epilepsy, cerebral palsy or cognitive and motor retardation.


Subject(s)
Humans , Male , Infant, Newborn , Zika Virus , Microcephaly , Stem Cells , Pregnancy , Diagnostic Imaging , Fever , Anti-Bacterial Agents
SELECTION OF CITATIONS
SEARCH DETAIL