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1.
Braz. dent. sci ; 25(2): 1-9, 2022. tab
Article in English | LILACS, BBO | ID: biblio-1369265

ABSTRACT

Objetivo: existem evidências inconclusivas sobre a potencial carogenicidade das fórmulas lácteas em comparação com o leite materno. O estudo teve como objetivo comparar a detecção e contagem de Streptococcus mutans(S. mutans) e lactobacilos da saliva entre lactentes alimentados com leite materno (B), com fórmulas de leite puro (France Lait-FL) e suplementada com probióticos (Nan 1 optipro-N), e avaliar crescimento in vitro dessas bactérias em amostras de leite materno e fórmulas lácteas. Material e Métodos: amostras salivares foram obtidas com swabs de algodão estéreis de 60 lactentes que foram agrupados de acordo com o tipo de leite. As amostras foram cultivadas para obter a frequência de detecção e contagens bacterianas. Para a investigação in vitro, sete amostras de leite materno doado e sete amostras recém-preparadas de ambas as fórmulas lácteas foram inoculadas por ambas as bactérias e então cultivadas para avaliar o crescimento bacteriano. Resultados:lactobacilos foram detectados em todos os lactentes, enquanto não foram encontradas diferenças significativas na detecção de S. mutans entre os grupos. As contagens de ambos os microrganismos na saliva foram menores em (B), enquanto uma diferença insignificante foi encontrada entre (B) e (N). Diferenças significativas foram evidentes nas contagens bacterianas in vitro sendo mais baixas em (B) seguido por (N) e (FL). Conclusão: o leite materno e as fórmulas lácteas com suplementos probióticos podem ter um papel protetor contra a cárie dentária em lactentes. (AU)


Objective: inconclusive evidence exists regarding potential cariogenicity of milk formulas compared to breast milk. The study aimed to compare Salivary Streptococcus mutans (S. mutans) and lactobacilli detection and counts among breastfed (B), plain formula (France Lait 1) (FL) and probiotic supplemented formula (Nan 1 optipro) (N) infants and to assess in-vitro growth of these bacteria in breast milk and milk formula samples. Material and Methods:salivary samples were obtained using sterile cotton swabs from 60 infants that were grouped according to nursing milk type. Samples were cultured to obtain the detection frequency and bacterial counts. For the in-vitro investigation, seven donated breast milk samples and seven freshly prepared samples of both milk formulas were inoculated by both bacteria and then cultured to assess bacterial growth. Results:lactobacilli were detected in all infants, while no significant differences were found in S. mutans detection among groups. Counts of both microorganisms in saliva were lowest in (B) while, insignificant difference was found between (B) and (N). Significant differences were evident in in-vitro bacterial counts being lowest in (B) followed by (N) and (FL). Conclusion: breast milk and probiotic supplement infants' milk formulas may have a protective role against dental caries in infants.(AU)


Subject(s)
Humans , Infant , Streptococcus mutans , Probiotics , Dental Caries , Infant Formula , Lactobacillus , Milk, Human
2.
Rev. cuba. med. trop ; 73(2): e607, 2021. tab, graf
Article in Spanish | LILACS, CUMED | ID: biblio-1347484

ABSTRACT

Introducción: Streptococcus mutans participa en el origen y progreso de la caries dental, una de las enfermedades más prevalentes a nivel mundial. Su control requiere métodos seguros y accesibles para la población. Myrciaria dubia (Kunth) McVaugh (Myrtaceae) (camu camu) es un árbol nativo de la amazonía peruana. La capacidad antimicrobiana de los componentes de su fruto ya se ha comprobado. Objetivo: Evaluar la actividad antibacteriana in vitro del extracto hidroetanólico de M. dubia contra S. mutans ATCC 35658. Métodos: Investigación experimental con posprueba y grupos controles. El extracto de la pulpa del fruto de M. dubia se obtuvo mediante maceración hidroetanólica. Las concentraciones evaluadas fueron 25 mg/mL, 50 mg/mL y 75 mg/mL. La capacidad antibacteriana se determinó mediante el método de difusión en disco. Gluconato de clorhexidina 0,12 por ciento fue el control positivo y el dimetilsulfóxido al 1 por ciento el control negativo. Resultados: La actividad antibacteriana se incrementó de manera directamente proporcional a la concentración del extracto. La concentración de 75 mg/mL mostró una media de inhibición de 18,2 ± 0,774 mm, seguido de la concentración de 50 mg/mL con una media de inhibición de 14,6 ± 1,055 mm y la concentración de 25 mg/mL con un halo de inhibición promedio de 10,1± 0,833 mm. La zona de inhibición del control positivo fue de 16,5 ± 0,516 mm. Existe diferencia estadísticamente significativa entre la concentración de 75 mg/mL y el control positivo (p< 0,05). Conclusiones: El extracto hidroetanólico de M. dubia muestra actividad antibacteriana in vitro de tipo bactericida sobre S. mutans ATCC 35668(AU)


Introduction: Streptococcus mutans is involved in the genesis and progress of dental caries, one of the most prevalent diseases worldwide, whose control requires safe methods accessible to the population. Myrciaria dubia (Kunth) McVaugh (Myrtaceae) (camu camu) is a tree native to the Peruvian Amazon. The antimicrobial capacity of the components of its fruit has already been verified. Objective: Evaluate the in vitro antibacterial activity of M. dubia hydroethanolic extract against S. mutans ATCC 35658. Methods: An experimental study was conducted with post-test analysis and control groups. The extract from the pulp of the fruit of M. dubia was obtained by hydroethanolic maceration. The concentrations evaluated were 25 mg/mL, 50 mg/mL and 75 mg/mL. Antibacterial capacity was determined by the disc diffusion method. The positive control was 0.12 percent chlorhexidine gluconate, whereas the negative control was 1 percent dimethyl sulfoxide. Results: The antibacterial activity increased directly proportional to the concentration of the extract. The concentration of 75 mg/mL showed a mean inhibition of 18.2 ± 0.774 mm, followed by the concentration of 50 mg/mL with a mean inhibition of 14.6 ± 1.055 mm and the concentration of 25 mg/mL with an average inhibition halo of 10.1 ± 0.833 mm. The zone of inhibition of the positive control was 16.5 ± 0.516 mm. There is a statistically significant difference between the concentration of 75 mg / mL and the positive control (p< 0.05). Conclusions: The M. dubia hydroethanolic extract displays in vitro antibacterial bactericidal activity against S. mutans ATCC 35668(AU)


Subject(s)
Humans , Male , Female , Streptococcus mutans , Myrtaceae , Dental Caries , Herpes Zoster , Anti-Bacterial Agents , In Vitro Techniques
3.
Rev. cienc. salud (Bogotá) ; 19(1): 88-98, ene.-abr. 2021. tab
Article in Spanish | LILACS | ID: biblio-1289168

ABSTRACT

Resumen Objetivo: evaluar el efecto antibacteriano del peróxido de hidrógeno (H2O2) al 6 % comparado con hipoclorito de sodio (NaClO) al 1 % y al 2 %, sobre cepillos dentales inoculados con Streptococcus mutans ATCC® 25175™. Materiales y métodos: estudio experimental in vitro, transversal y comparativo. Se utilizaron 60 cepillos dentales, divididos en 4 grupos de 15 cepillos cada uno. El número de muestra lo determinó la fórmula de comparación de medias, después de realizar un estudio piloto, y se seleccionaron por muestreo aleatorio simple. Se aplicaron las pruebas de normalidad de Shapiro-Wilk, y para la prueba de hipótesis, la U de Mann-Whitney. Resultados: el H2O2 al 6 % presentó una media de crecimiento de 2 * 109 UFC/mL, lo que indica que su efectividad es mayor comparada con el NaClO al 1 %>, que presentó una media de crecimiento de 4 * 109 UFC/mL, y esta es menor al NaClO al 2 %, que tuvo 0 UFC /mL de Streptococcus mutans (p = 0,004). Conclusión: el H2O2 al 6 % y el NaClO al 1 % y al 2 % evidenciaron efectividad antibacteriana, aun cuando fue el NaClO al 2 % el más efectivo.


Abstract Objective: To assess the antibacterial effect of 6% hydrogen peroxide (H2O2) compared with 1% and 2% sodium hypochlorite, on toothbrushes inoculated with Streptococcus mutans ATCC® 25175™. Materials and methods: The study design was an experimental in vitro, cross-sectional prospective and comparative study. Sixty toothbrushes were used, which were divided into four groups of 15 brushes. After conducting a pilot study, the sample number was determined by the means comparison formula and these were selected by simple random sampling. These brushes were inoculated with strains of S. mutans ATCC® 25175™. The disinfectants included H2O2 at 6% and sodium hypochlorite (NaOCl) at 1% and 2%. The Shapiro-Wilk test was used to assess normality and the Mann-Whitney U test was applied for the hypothesis test. Results: The 6% H2O2 showed an average growth of 2 * 109 CFU/mL, which indicates that its effectiveness is greater compared to the 1% NaClO that showed a growth average of 4 * 109 CFU/mL which is less than the 2% NaClO that presented 0 CFU/mL of S. mutans (p = 0.004). Conclusion: Between 6% H2O2 and 1% and 2% NaClO, specifically antibacterial detection, 2% NaClO was concluded as being the most effective.


Resumo Objetivo: avaliar o efeito antibacteriano do peróxido de hidrogênio 6% em comparação ao hipoclorito de sódio 1% e 2%, em escovas de dente inoculadas com Streptococcus mutans ATCC® 25175™. Materiais e métodos: estudo experimental in vitro, transversal e comparativo. Foram utilizadas 60 escovas de dente, as quais foram divididas em 4 grupos de 15 escovas cada um. O número amostral foi determinado pela fórmula de comparação de médias, após realização de um estudo piloto, sendo selecionado por meio de amostragem aleatória simples. As escovas foram inoculadas com cepas de Streptococcus mutans ATCC® 25175™. Os desinfetantes utilizados foram peróxido de hidrogênio (H202) 6% em comparação ao hipo-clorito de sódio (NaClO) 1% e 2%. Aplicou-se a prova de normalidade de Shapiro-Wilk e como teste de hipótese utilizou-se o U de Mann-Whitney. Resultados: o H202 6% apresentou uma média de crescimento de 2*109 UFC/mL, indicando uma efetividade maior em comparação com o NaClO 1% que apresentou uma média de crescimento de 4*109 UFC/mL, que por sua vez foi menos efetivo que o NaClO 2% que apresentou uma contagem de 0 UFC/mL de Streptococcus mutans (p=0,004). Conclusão: o H2O2 6% e o NaClO 1% e 2%, apresentaram efetividade antibacteriana, sendo que o NaClO 2% foi totalmente efetivo.


Subject(s)
Humans , Sodium Hypochlorite , Streptococcus mutans , Hydrogen Peroxide , Disinfection
4.
Braz. dent. sci ; 24(1): 1-7, 2021. tab, ilus
Article in English | LILACS, BBO | ID: biblio-1145548

ABSTRACT

Though aloe vera extract, green tea extract and coriander oil are proven antimicrobial agents, very little information is available regarding its effects on oral bacteria, Streptococcus mutans, which is responsible for initiating caries and Enterococcus faecalis, responsible for failure of root canal treatment. Objective: To find the antimicrobial activity of aloe vera extract, black tea extract and coriander oil against S. mutans and E. faecalis. Materials and Methods: The agar well diffusion method was used to determine the antibacterial activity of Aloe vera extract, black tea extract and coriander oil. Different concentration of prepared plant extracts and coriander seed oil (50 & 100 µl) was incorporated into the wells and the plates containing S. mutans and E. faecalis were incubated at 37 °C for 24 h. The antibiotic (amoxicillin 30 µl) was used as positive control. Zone Of Inhibition (ZOI) was recorded in each plate. Results: For S. mutans, the maximum ZOI was created by coriander oil with a diameter of 25.00±0.58 mm at 50 µl and for E. faecalis, maximum ZOI was created by aloe vera extract 16.00±0.58 mm at 100 µl concentration which were far better than the control: amoxicillin 30 µl concentration. Conclusion: The extracts of Aloe vera, black tea and coriander oil, showed significant activity against the investigated microbial strains, Streptococcus mutans and Enterococcus faecalis which further helps in the development of new topical agents that help in reducing the numbers of these organisms present in the oral cavity. (AU)


Embora o extrato de aloe vera, extrato de chá verde e óleo de coentro sejam agentes antimicrobianos comprovados, há pouca informação disponível sobre seus efeitos nas bactérias orais, Streptococcus mutans, que é responsável por iniciar cáries e Enterococcus faecalis, responsável pela falha do tratamento de canal radicular. Objetivo: Avaliar a atividade antimicrobiana do extrato de aloe vera, extrato de chá preto e óleo de coentro contra S. mutans e E. faecalis. Materiais e Métodos: O método de difusão em agar foi usado para determinar a atividade antibacteriana do extrato de Aloe vera, extrato de chá preto e óleo de coentro. Diferentes concentrações dos extratos de plantas e óleo de semente de coentro (50 e 100 µl) foram preparados e colocados nos poços e nas placas contendo S. mutans e E. faecalis e foram incubadas a 37°C por 24 h. O antibiótico (amoxicilina 30 µl) foi utilizado como controle positivo. A zona de inibição (ZOI) foi registrada em cada placa. Resultados: Para S. mutans, a ZOI máxima foi obtida com o óleo de coentro com um diâmetro de 25,00 ± 0,58 mm a 50 µl e para E. faecalis, a ZOI máxima foi obtiada pelo extrato de aloe vera 16,00 ± 0,58 mm na concentração de 100 µl, as quais foram melhores do que o controle: concentração de 30 µl de amoxicilina. Conclusão: Os extratos de Aloe vera, chá preto e óleo de coentro apresentaram atividade significativa contra as cepas microbianas investigadas, Streptococcus mutans e Enterococcus faecalis auxiliando no desenvolvimento de novos agentes tópicos visando a redução do número desses organismos presentes no cavidade oral. (AU)


Subject(s)
Streptococcus mutans , Tea , Enterococcus faecalis , Aloe , Microbiota
5.
Article in English | WPRIM | ID: wpr-922687

ABSTRACT

Glucosyltransferases (Gtfs) play critical roles in the etiology and pathogenesis of Streptococcus mutans (S. mutans)- mediated dental caries including early childhood caries. Gtfs enhance the biofilm formation and promotes colonization of cariogenic bacteria by generating biofilm extracellular polysaccharides (EPSs), the key virulence property in the cariogenic process. Therefore, Gtfs have become an appealing target for effective therapeutic interventions that inhibit cariogenic biofilms. Importantly, targeting Gtfs selectively impairs the S. mutans virulence without affecting S. mutans existence or the existence of other species in the oral cavity. Over the past decade, numerous Gtfs inhibitory molecules have been identified, mainly including natural and synthetic compounds and their derivatives, antibodies, and metal ions. These therapeutic agents exert their inhibitory role in inhibiting the expression gtf genes and the activities and secretion of Gtfs enzymes with a wide range of sensitivity and effectiveness. Understanding molecular mechanisms of inhibiting Gtfs will contribute to instructing drug combination strategies, which is more effective for inhibiting Gtfs than one drug or class of drugs. This review highlights our current understanding of Gtfs activities and their potential utility, and discusses challenges and opportunities for future exploration of Gtfs as a therapeutic target.


Subject(s)
Biofilms , Dental Caries/prevention & control , Glucosyltransferases/antagonists & inhibitors , Humans , Streptococcus mutans/enzymology
6.
Article in English | WPRIM | ID: wpr-922472

ABSTRACT

Streptococcus mutans (S. mutans) is generally regarded as a major contributor to dental caries because of its ability to synthesize extracellular polysaccharides (EPS) that aid in the formation of plaque biofilm. The VicRKX system of S. mutans plays an important role in biofilm formation. The aim of this study was to investigate the effects of vicK gene on specific characteristics of EPS in S. mutans biofilm. We constructed single-species biofilms formed by different mutants of vicK gene. Production and distribution of EPS were detected through atomic force microscopy, scanning electron microscopy and confocal laser scanning microscopy. Microcosmic structures of EPS were analyzed by gel permeation chromatography and gas chromatography-mass spectrometry. Cariogenicity of the vicK mutant was assessed in a specific pathogen-free rat model. Transcriptional levels of cariogenicity-associated genes were confirmed by quantitative real-time polymerase chain reaction. The results showed that deletion of vicK gene suppressed biofilm formation as well as EPS production, and EPS were synthesized mostly around the cells. Molecular weight and monosaccharide components underwent evident alterations. Biofilms formed in vivo were sparse and contributed a decreased degree of caries. Moreover, expressional levels of genes related to EPS synthesis were down-regulated, except for gtfB. Our report demonstrates that vicK gene enhances biofilm formation and subsequent caries development. And this may due to its regulations on EPS metabolism, like synthesis or microcosmic features of EPS. This study suggests that vicK gene and EPS can be considered as promising targets to modulate dental caries.


Subject(s)
Animals , Biofilms , Dental Caries , Dental Plaque , Rats , Streptococcus mutans/genetics
7.
Article in Chinese | WPRIM | ID: wpr-878429

ABSTRACT

OBJECTIVES@#To evaluate the effects of antimicrobial peptide GH12 designed @*METHODS@#The cariogenic three-species biofilm consis-ted of the cariogenic @*RESULTS@#The biomass and density of the cariogenic three-species biofilm treated with GH12 decreased compared with those of the control. The number of @*CONCLUSIONS@#GH12 can reduce the number of


Subject(s)
Biofilms , Dental Caries , Humans , In Situ Hybridization, Fluorescence , Pore Forming Cytotoxic Proteins , Streptococcus mutans
8.
J. appl. oral sci ; 29: e20200778, 2021. tab, graf
Article in English | LILACS | ID: biblio-1340096

ABSTRACT

Abstract Objective this study evaluated the mineral and microbiological response of biofilms originating from different types of saliva inoculum with distinct levels of caries activity. Methodology the biofilms grown over enamel specimens originated from saliva collected from a single donor or five donors with two distinct levels of caries activity (caries-active and caries-free) or from pooling saliva from ten donors (five caries-active and five caries-free). The percentage surface hardness change (%SHC) and microbiological counts served as outcome variables. Results the caries activity of donors did not affect the %SHC values. Inoculum from five donors compared to a single donor showed higher %SHC values (p=0.019). Higher lactobacilli counts were observed when saliva from caries-active donors was used as the inoculum (p=0.017). Pooled saliva from both caries activity levels showed higher mutans streptococci counts (p<0.017). Conclusion Overall, pooled saliva increased the mineral response of the derived biofilms, but all the inoculum conditions formed cariogenic biofilms and caries lesions independently of caries activity.


Subject(s)
Humans , Saliva , Dental Caries , Streptococcus mutans , Biofilms , Dental Caries Susceptibility , Minerals
9.
J. appl. oral sci ; 29: e20201031, 2021. tab, graf
Article in English | LILACS | ID: biblio-1250189

ABSTRACT

Abstract Fixed orthodontic appliances may lead to biofilm accumulation around them that may increase caries risk. This study aimed to evaluate the influence of quaternary ammonium methacrylates (QAMs) on the physicochemical properties, cytotoxicity, and antibacterial activity of adhesive resins for orthodontic purposes. Methodology: A base resin was prepared with a comonomer blend and photoinitiator/co-initiator system. Two different QAMs were added to the base adhesive: dimethylaminododecyl methacrylate at 5 wt.% (DMADDM) or dimethylaminohexadecyl methacrylate (DMAHDM) at 10 wt.%. The base adhesive, without QAMs, (GC) and the commercial Transbond™ XT Primer 3M (GT) were used as control. The resins were tested immediately and after six months of aging in the water regarding the antibacterial activity and shear bond strength (SBS). The antibacterial activity was tested against Streptococcus mutans via metabolic activity assay (MTT test). The groups were also tested for the degree of conversion (DC) and cytotoxicity against keratinocytes. Results: The resins containing QAM showed antibacterial activity compared to the commercial material by immediately reducing the metabolic activity by about 60%. However, the antibacterial activity decreased after aging (p<0.05). None of the groups presented any differences for SBS (p>0.05) and DC (p>0.05). The incorporation of DMADDM and DMAHDM significantly reduced the keratinocyte viability compared to the GT and GC groups (p<0.05). Conclusion: Both adhesives with QAMs showed a significant reduction in bacterial metabolic activity, but this effect decreased after water aging. Lower cell viability was observed for the group with the longer alkyl chain-QAM, without significant differences for the bonding ability and degree of conversion. The addition of QAMs in adhesives may affect the keratinocytes viability, and the aging effects maybe decrease the bacterial activity of QAM-doped materials.


Subject(s)
Orthodontic Brackets , Streptococcus mutans , Materials Testing , Biofilms , Resin Cements , Dental Cements , Methacrylates , Anti-Bacterial Agents
10.
Article in English | LILACS, BBO | ID: biblio-1143394

ABSTRACT

ABSTRACT Objective: To analyze the ability of saliva in controlling the growth and the biofilm formation of Streptococcus mutans (S. mutans) as well as the effect of histatin-5 anti-biofilm relate to pH and saliva viscosity. Material and Methods: The S. mutans biofilm assayed by crystal violet 1% and its growth measured by spectrophotometer. The saliva viscosity was analyzed by viscometer, and pH of saliva was measured by pH meter. Results: Based on the optical density values, growth of S. mutans in saliva ranged <300 CFU/mL (0.1 nm) at concentrations of 25%, 12.5% and 6.25% for 24 hours. Whereas at the 48 h and 72 h period of incubation shown an increase in growth of S. mutans ranged 300-600 CFU/mL (0.2-0.36 nm). The inhibitory biofilm formation of S. mutans in saliva was significantly higher at concentrations of 12.5% and 6.25% at 24 h incubation times on a moderate scale, whereas the histatin-5 was effective to inhibit S. mutans biofilm on the 50 and 25 ppm. The saliva possessed a higher inhibitory of biofilm S. mutans than histatin-5 and good level viscosity (0.91-0.92 cP). Conclusion: The saliva was able to control the growth of S. mutans, and histatin-5 can inhibit the biofilm formation S. mutans. Furthermore, the saliva was also able to respond to the pH change with good viscosity of saliva.


Subject(s)
Humans , Male , Female , Child , Saliva/microbiology , Biofilms , Viridans Streptococci , Histatins , Hydrogen-Ion Concentration , Spectrophotometry/instrumentation , Streptococcus mutans , Viscosity , Analysis of Variance , Statistics, Nonparametric , Indonesia/epidemiology
11.
Article in English | LILACS, BBO | ID: biblio-1287491

ABSTRACT

Abstract Objective: To determine the level of biofilm formation of S. mutans after being exposed to 5% sucrose, 8% lactose, or 1% xylitol. Material and Methods: This research was a laboratory-based experimental study with post-test only control group design. S. mutans was grown in test tubes containing tryptose soy broth (TSB) medium supplemented with 1% glucose. They were incubated at 37° C for 24 hours to grow the biofilms. The culture was then exposed to 5% sucrose, 8% lactose or 1% xylitol, incubated for 24 hours at 37° C, and examined using ELISA at a wavelength of 625 nm. The statistical analysis was performed using a one-way analysis of variance followed by the least significant difference test (a=0.05). Results: There were some differences in the biofilm formation of S. mutans after exposure to 5% sucrose, 8% lactose, or 1% xylitol (p<0.05). An LSD test indicated significant differences among the biofilm formations after exposure to 5% sucrose and 8% lactose and between 5% sucrose and 1% xylitol. In comparison, there were no significant differences (p>0.05) between 8% lactose and 1% xylitol. Conclusion: Sucrose, lactose and xylitol can form biofilms and the formation of lactose biofilms is the same as xylitol.


Subject(s)
Streptococcus mutans/immunology , Sucrose/adverse effects , Xylitol , Disaccharides , Indonesia/epidemiology , Enzyme-Linked Immunosorbent Assay , Epidemiology, Experimental , Analysis of Variance , Biofilms , Dental Plaque
12.
Article in English | LILACS, BBO | ID: biblio-1287481

ABSTRACT

ABSTRACT Objective: To investigate the antibacterial, mechanical, physical properties and water sorption of flowable dental composites containing 3,4-dihydropyrimidin-2(1H)-ones. Material and Methods: 3,4-dihydropyrimidin-2(1H)-ones was synthesized and the antibacterial activity of flowable dental composites containing 0-5 wt% 3,4-dihydropyrimidin-2(1H)-ones and also of their mechanical and physical properties on flowable dental composites were investigated. Flexural strength was measured by a three-point bending test. Compressive strength (CS), Water sorption (WS) and depth of cure (DOC) were investigated. The data were analyzed by One-way ANOVA test. The level of significance was determined as p<0.01. Results: The direct contact test demonstrates that by increasing the 3,4-dihydropyrimidin-2(1H)-ones content, the bacterial growth is significantly diminished (p<0.001). The average flexural strength results show that with increasing 3,4-dihydropyrimidin-2(1H)-ones until 3% in the composite, no significant difference was observed in flexural strength (p>0.001) and the mean of compressive strength results show no significant difference between 0-4% groups (p>0.001). The mean of water sorption and depth of cure results shows no significant difference between groups (p>0.001). Conclusion: Incorporation of 3,4-dihydropyrimidin-2(1H)-ones into flowable resin composites in 3% wt can reduce the activity of Streptococcus mutans.


Subject(s)
Streptococcus mutans/immunology , Microbial Sensitivity Tests , Composite Resins , Compressive Strength , Anti-Bacterial Agents/immunology , Analysis of Variance , Sorption Detoxification , Physical and Chemical Properties , Flexural Strength , Iran
13.
Article in English | LILACS, BBO | ID: biblio-1250450

ABSTRACT

ABSTRACT Objective: To investigate the antimicrobial activity of colloidal selenium nanoparticles in chitosan solution (Cts-Se-NPs) against Streptococcus mutans, Lactobacillus acidophilus, and Candida albicans. Material and Methods: Cts-Se-NPs solution was prepared using a simple chemical reduction method. The MIC and MBC against S. mutans, L. acidophilus, and C. albicans were determined using the broth dilution assay. Results: The Cts-Se-NPs had remarkable antimicrobial activity against S. mutans, L. acidophilus, and C. albicans. The MIC values of the Cts-Se-NPs were lowest for S. mutans (0.068 mg/ml) compared to L. acidophilus (0.137 mg/ml), and C. albicans (0.274 mg/ml). The MBC values of the Cts-Se-NPs against the microorganisms after one, two, six, and 24 hours indicated that the concentration of 0.274 mg/ml of Cts-Se-NPs completely killed S. mutans, L. acidophilus, and C. albicans after one, two, and six hours, respectively. At the concentration of 0.137 mg/ml, S. mutans and L. acidophilus were killed after six and 24 hours, respectively. Conclusion: These findings encourage the potential use of Cts-Se-NPs in dentistry, while further clinical research is required in this area.


Subject(s)
Selenium , Streptococcus mutans , Dentistry , Chitosan , Nanoparticles , Candida albicans , Iran , Lactobacillus acidophilus
14.
Article in English | LILACS, BBO | ID: biblio-1180862

ABSTRACT

ABSTRACT Objective: To identify etiologic microbiota associated periodontal diseases among diabetes patients and the factors related to the most commonly identified bacteria species. Material and Methods: Periodontal plaque samples from 11 diabetic participants and 13 non-diabetic controls were collected to assess their aerobic and anaerobic bacterial growth. Different distinct colonies were identified by microscopic and 16srDNA sequencing. Pearson's chi-square tests were conducted to examine any association between categorical variables. Results: The diabetic subjects revealed a more intense plaque formation with a mean plaque index of 2.4 compared to 1.8 in non-diabetics. A total of 86 bacteria were isolated from 24 plaque samples, 44 were aerobic, and 42 were anaerobic. Only aerobic isolates, 22 from diabetic patients and 22 from non-diabetic patients, were evaluated in these analyses. Bacillus spp. (B. cereus mainly) and Klebsiella spp. (K. pneumoniae, K. aerogenes, K. oxytoca) were detected markedly higher in non-diabetic individuals than in diabetic subjects (p=0.026 and p=0.021, respectively). Some bacteria were only identified in the dental plaque of diabetic individuals, namely, Bacillus mojavensis, Enterobacter cloacae, Proteus mirabilis, Staphylococcus epidermidis, Staphylococcus hominis, Staphylococcus pasteuri, Streptococcus mutans, and Streptococcus pasteurianus. The presence of acid reflux and jaundice were significantly associated with the most common bacterial isolate, namely Bacillus spp., with the p-values of 0.007 and 0.001, respectively. Conclusion: Type-2 diabetes mellitus is associated with a higher amount of dental plaques. Periodontal plaque samples from diabetic and non-diabetic subjects possess differential microbial communities. Diabetic plaques contain more versatile microbes predominated by gram-positive streptococci and staphylococci.


Subject(s)
Humans , Male , Female , Child , Adolescent , Adult , Middle Aged , Aged , Periodontal Diseases/etiology , Periodontitis/pathology , Oral Health/education , Diabetes Mellitus, Type 2/microbiology , Microbiota/immunology , Streptococcus mutans/immunology , Bangladesh/epidemiology , Radiography, Dental/instrumentation , Chi-Square Distribution , Dental Care , Dental Plaque , Diabetes Mellitus/microbiology
15.
São José dos Campos; s.n; 2021. 116 p. ilus, tab, graf.
Thesis in Portuguese | LILACS, BBO | ID: biblio-1362020

ABSTRACT

Os objetivos deste estudo foram comparar três materiais bioativos a uma resina bulk fill convencional (controle), quanto à adesão bacteriana e alterações ópticas, quando expostos a solução corante e escovação simulada; como também, avaliar o esmalte adjacente a restaurações com esses materiais, quando submetidas ao envelhecimento térmico e a desafio cariogênico. Para determinação da adesão bacteriana (fase 1), foram confeccionadas 10 amostras de cada grupo: AB: Activa Bioactive Restorative (PulpdentTM Corporation, USA); BB: Beautifil Bulk (Shofu inc., Kyoto, Japan); EQ: Equia Forte (GC América Inc., Illinois, EUA) e FBC: Filtek Bulk Fill (3M ESPE, Saint Paul, Minessota, EUA 3M). Após a polimerização, as amostras foram armazenadas por 48h e então expostas a cepa padrão de Streptococcus mutans (UA 159) e determinado o número de unidades formadoras de colônia (UFC/ml). Foram então autoclavadas e submetidas diariamente a ciclos de pigmentação por café e escovação simulada, durante 30 dias, seguidos de uma segunda adesão bacteriana. Alterações de cor e translucidez foram avaliadas após esses ciclos. Os dados foram submetidos à ANOVA dois fatores e teste de Tukey (5%). Para avaliação do esmalte (fase 2), foram utilizadas as faces vestibulares e linguais de 50 terceiros molares humanos distribuídas aleatoriamente em 5 grupos (n=20): grupo EH: esmalte hígido (controle) e, 4 grupos que receberam preparos cavitários padronizados restaurados com os mesmos materiais utilizados na fase 1. Os espécimes foram expostos à termociclagem (10.000 ciclos) e desafio cariogênico. A microdureza do esmalte foi medida inicialmente, após termociclagem e após o desafio cariogênico. Os dados foram submetidos a ANOVA dois fatores de medidas repetidas e teste de Tukey (5%). Os resultados apresentaram diferenças estatisticamente significantes entre os grupos para as adesões bacterianas; sendo que na primeira, o grupo AB (6,2 log) apresentou o menor valor. Na segunda adesão, o grupo FBC manteve seu crescimento estável (7,5 log). Os grupos AB e EQ (9,4 e 9,2 log) apresentaram médias superiores as da primeira adesão (6,2 e 7,6 log respectivamente). O grupo BB (6,7 log) mostrou a menor adesão bacteriana. Diferenças na cor não foram estatisticamente significantes entre os grupos, no entanto, todos materiais mostraram alteração de cor perceptível (∆E> 2,7) após os ciclos de exposição ao café e escovação. A translucidez foi estatisticamente diferente entre os grupos, mas não foi influenciada pelos ciclos. A microdureza do esmalte diminuiu para todos os grupos após a termociclagem. Os grupos FBC e EH apresentaram as menores médias de microdureza (178,78/ 202,83 kgf); os grupos AB e BB (240,82/ 265,34 kgf), foram estatisticamente semelhantes; e o grupo EQ apresentou a maior média (274,18 kgf). Uma diminuição da microdureza aconteceu após o desafio cariogênico, exceto para o grupo EQ (244,73 kgf). Os grupos FBC e EH (117,18/ 112,97 kgf), não apresentaram diferenças estatísticas entre eles, com as menores médias de microdureza; e, os grupos BB e AB (211,32/ 171,14 kgf) apresentaram diferenças estatísticas em relação aos demais grupos. Podemos concluir que: os materiais bioativos interferiram na adesão bacteriana por Streptococcus mutans, e foram susceptíveis aos ciclos de pigmentação e escovação; a imersão no café e escovação simulada alteraram a cor, mas não a translucidez de todos os materiais; a termociclagem reduziu a dureza superficial do esmalte adjacente a todas as restaurações nesse estudo; e que, o desafio cariogêncio reduziu a dureza do esmalte exceto para o grupo EQ, que apresentou manutenção da sua dureza, mostrando seu potencial de influenciar a resistência do esmalte à desmineralização.


The aim of this study was to compare three bioactive materials to a conventional resin, regarding bacterial adhesion and optical changes, when exposed to dye solution and simulated brushing; as well as evaluate the enamel adjacent to restorations with these materials, when submitted to thermal aging and cariogenic challenge. To determine bacterial adhesion (phase 1), 10 samples of each material were prepared: AB: Activa Bioactive Restorative (Pulpdent™ Corporation, USA); BB: Beautifil Bulk (Shofu inc., Kyoto, Japan); EQ: Equia Forte (GC America Inc., Illinois, USA) and FBC: Filtek Bulk Fill (3M ESPE, Saint Paul, Minnesota, USA 3M). After 48 hours the samples were exposed to a standard strain of Streptococcus mutans (UA 159) and the number of colony forming units (CFU/ml) determined. They were then autoclaved and submitted daily to coffee pigmentation and simulated brushing cycles, for 30 days, followed by a second bacterial adhesion. Color and translucency changes were assessed after these cycles. Data were submitted to two-way ANOVA and Tukey test (5%). For enamel evaluation (phase 2), buccal and lingual surfaces of 50 human third molars were randomly distributed into 5 groups (n=20): EH group: sound enamel (control) and 4 groups that received standardized cavity preparations restored with the same materials in phase. Specimens were exposed to thermocycling (10,000 cycles) and cariogenic challenge. Enamel microhardness was measured initially, after thermocycling and after cariogenic challenge. Data were submitted to Two-way Repeated Measures ANOVA and Tukey test (5%). Bacterial adhesion results showed statistically significant differences among groups; in the first adhesion, AB group (6.2 log) presented the lowest bacterial growth. In the second one, group FBC maintained its growth (7.5 log). AB and EQ groups (9.3 log) presented higher growth than before the treatments (6.2 and 7.6 log respectively). BB group (6.7 log) showed the lowest bacterial adhesion. Color differences were not statistically significant among groups; however, noticeable color change (∆E> 2.7) was seen for all materials after the coffee and brushing cycles. Translucency was statistically different among the groups but was not influenced by the cycles. Enamel microhardness decreased for all groups after thermocycling. FBC group and EH group, showed the lowest microhardness averages (178.78/202.83 kgf); AB and BB groups (240.82/265.34 kgf) were statistically similar; and EQ group had the highest average (274.18 kgf). A decrease in microhardness occurred after the cariogenic challenge for all materials, except for the EQ group (244.73 kgf). FBC and EH groups (117.18/112.97 kgf) did not present statistical differences between them, showing the lowest microhardness averages; and, groups BB and AB (211.32/ 171.14 kgf), were statistically different to the other groups. We can conclude that: bioactive materials interfered with bacterial adhesion by Streptococcus mutans, and were susceptible to pigmentation and brushing cycles; immersion in coffee and simulated brushing changed color, but not translucency for all materials; thermocycling reduced surface hardness of the enamel adjacent to all restorations in this study; and that cariogenic challenge also reduced enamel hardness, except for the EQ group, which maintained its hardness, showing its potential to influence enamel resistance demineralization.


Subject(s)
Humans , Streptococcus mutans , Tooth Remineralization , Biocompatible Materials , Dental Caries , Resins, Synthetic , Pigmentation , Analysis of Variance , Color
16.
J. Health Biol. Sci. (Online) ; 9(1): 1-7, 2021. tab
Article in English | LILACS | ID: biblio-1362963

ABSTRACT

Objective: When provisional acrylic crowns are used for a long time, they become more susceptible to marginal leakage by cariogenic bacteria. The objectives of this pilot clinical study were to compare cement based on zinc oxide-eugenol and calcium hydroxide by contamination with Streptococcus mutans, and calculate the sample size for the continuation of this study. Methods: Individuals receiving provisional crowns and following inclusion/exclusion criteria, were randomly distributed into 2 groups: zinc oxide-eugenol (n=8); calcium hydroxide (n=9). The temporary crowns were made by a blind researcher and cemented by another. Patients were also blinded by the cement used inside their crowns. After 2 months, a cement sample from the crowns' peripheral inner face was collected, placed in a tube containing 1 mL of sterile saline, serially diluted, plated on Mitis Salivarius Bacitracin agar, and incubated for 48 hours. Colony-forming units (CFU/mL) were counted. A statistical power analysis was performed to calculate sample size (1-ß=80%) and the Mann Whitney test to compare both cements (α=0.05). Results: Both cements were contaminated with S. mutans, with an average of 166.6 x 102 CFU/mL for calcium hydroxide and 435.3 x 102 CFU/mL for zinc oxide-eugenol, with no significant difference (p=0.311). The sample size calculated for this study was 36 per group. Conclusion: This pilot study suggests that there is important contamination inside provisional crowns used for two months, independent of the cement. The continuation of this study is needed, with a bigger sample size, to enable a comparison between the cements.


Objetivo: Quando coroas dentais provisórias são utilizadas por um longo período, elas se tornam susceptíveis à infiltração marginal por bactérias cariogênicas. O objetivo deste estudo clínico piloto foi comparar os cimentos a base de óxido de zinco e eugenol e hidróxido de cálcio pela contaminação com Streptococcus mutans e calcular o tamanho amostral para continuação deste estudo. Métodos: Indivíduos recebendo coroas provisórias e seguindo critérios de inclusão/exclusão, foram distribuídos aleatoriamente em dois grupos: óxido de zinco e eugenol (n=8); hidróxido de cálcio (n=9). As coroas provisórias foram feitas por um pesquisador cego e cimentadas por outro. Os pacientes também foram cegos quanto ao cimento utilizado dentro de suas coroas. Depois de 2 meses, amostras de cimentos foram coletadas da face interna periférica das coroas, colocadas em um tubo contendo 1 mL de solução salina estéril, diluídas de forma seriada, plaqueadas em ágar Mitis Salivarius Bacitracina e incubadas por 48 horas. Unidades formadoras de colônias (UFC/mL) foram contadas. Um teste de poder estatístico foi realizado para calcular o tamanho amostral (1-ß=80%) e o teste de Mann Whitney para comparar os dois cimentos (α=0.05). Resultados: Os dois cimentos foram contaminados com S. mutans, com uma média de 166.6 x 102 UFC/mL para o hidróxido de cálcio e 435.3 x 102 UFC/mL para o óxido de zinco e eugenol, com nenhuma diferença significativa (p=0.311). O cálculo amostral para este estudo foi 36 indivíduos por grupo. Conclusão: Este estudo piloto sugere que existe importante contaminação dentro de coroas provisórias utilizadas por 2 meses, independente do cimento. A continuação deste estudo é necessária, com maior tamanho amostral, para possibilitar a comparação entre os cimentos.


Subject(s)
Streptococcus mutans , Dental Leakage , Zinc Oxide-Eugenol Cement , Calcium Hydroxide , Eugenol , Dental Prosthesis , Tooth Crown , Crowns , Dental Cements , Denture, Partial, Fixed , Clinical Study
17.
São José dos Campos; s.n; 2021. 59 p. ilus, graf, tab.
Thesis in Portuguese | LILACS, BBO | ID: biblio-1359953

ABSTRACT

Candida albicans possui capacidade de causar uma ampla variedade de manifestações clínicas devido a múltiplos fatores de virulência que agem simultaneamente para vencer o sistema imune e invadir os tecidos do hospedeiro. Estudos recentes demonstraram que o crescimento de C. albicans pode ser inibido por metabólitos produzidos por Streptococcus mutans, que estão presentes no sobrenadante da cultura bacteriana. Assim, o objetivo desse estudo foi investigar se o sobrenadante da cultura de S. mutans, além de inibir o crescimento de C. albicans, é capaz de atenuar os mecanismos de virulência desse fungo. Inicialmente, uma suspensão padronizada de S. mutans (107 células/mL) foi incubada em caldo BHI a 37ºC por 4 h em 5% de CO2. O crescimento em BHI foi filtrado em membrana de 0,22 µm, obtendo-se o sobrenadante da cultura de S. mutans livre de células. A seguir, uma suspensão padronizada de C. albicans (107 células/mL) foi adicionada ao sobrenadante filtrado da cultura de S. mutans em caldo BHI, sendo incubada a 37ºC por 24 h. Para os grupos controle, a suspensão de C. albicans foi colocada em caldo BHI ou YPD esterilizados e incubada nas mesmas condições. Após o período de incubação, o crescimento de C. albicans foi centrifugado e lavado para obtenção de uma suspensão padronizada de C. albicans contendo as células sobreviventes da exposição ao sobrenadante de S. mutans. Essa suspensão de C. albicans foi então utilizada nos testes in vitro e in vivo para determinação dos fatores de virulência desse micro-organismo. No estudo in vitro, foi investigada a atividade proteolítica extracelular de C. albicans, bem como sua capacidade de filamentação, adesão e formação de biofilmes (1:30, 6, 24 e 48 h). Para o estudo in vivo, foi utilizado o modelo de Galleria mellonella, analisando-se a curva de sobrevivência, o número de células fúngicas e hemócitos na hemolinfa de larvas infectadas por C. albicans. Para a análise estatística foi utilizado ANOVA, teste de Tukey, Kruskal-Wallis e Log-rank, com nível de significância de 5%. Verificou-se que as células de C. albicans expostas ao sobrenadante de S. mutans apresentaram redução na filamentação, formação de biofilmes e patogenicidade em G. mellonella em relação ao controle. A exposição ao sobrenadante de S. mutans também mudou o padrão de aderência de C. albicans. Entretanto, o sobrenadante de S. mutans não reduziu a atividade proteolítica de C. albicans. Concluiu-se que o sobrenadante de S. mutans apresentou capacidade de inibir importantes mecanismos de virulência de C. albicans, podendo ser uma fonte de novos agentes antifúngicos a ser explorada


Candida albicans has the ability to cause a wide variety of clinical manifestations due to multiple virulence factors that act simultaneously to overcome the immune system and invade host tissues. Recent studies have shown that the growth of C. albicans can be inhibited by metabolites produced by Streptococcus mutans. Thus, the objective of this study was to investigate whether the culture supernatant of S. mutans is able to attenuate the virulence mechanisms of this fungus. Initially, a standardized suspension of S. mutans (107 cells/mL) was incubated in BHI broth at 37ºC for 4 h in 5% CO2. The growth in BHI was filtered through a 0.22 µm membrane, obtaining the cell free culture supernatant of S. mutans. Then, a standardized suspension of C. albicans (107 cells/mL) was prepared and added to the supernatant of the culture of S. mutans in BHI broth, being incubated at 37ºC for 24 h. For the control groups, the suspension of C. albicans was placed in sterile BHI or YPD broth and incubated under the same conditions. After the incubation period, the growth of C. albicans was centrifuged and washed to obtain a standardized suspension of C. albicans containing the surviving cells from exposure to the S. mutans supernatant. This suspension of C. albicans was then used to perform in vitro and in vivo tests to determine the virulence factors of this microorganism. In the in vitro study, the extracellular proteolytic activity of C. albicans was investigated, as well as its capacity for filamentation, adhesion and biofilm formation (1:30, 6, 24 and 48 h). For the in vivo study, the Galleria mellonella model was used, analyzing the survival curve, the number of fungal cells and hemocytes in the hemolymph of larvae infected with C. albicans. For statistical analysis, ANOVA, Tukey's test, Kruskal-Wallis and Log-rank were used, with a significance level of 5%. It was found that the cells of C. albicans exposed to the supernatant of S. mutans showed a reduction in filament, formation of biofilms and pathogenicity in G. mellonella in relation to the control. Exposure to the S. mutans supernatant also changed the pattern of adherence of C. albicans. However, the S. mutans supernatant did not reduce the proteolytic activity of C. albicans. It was concluded that the supernatant of S. mutans had the capacity to inhibit important mechanisms of virulence of C. albicans, being able to be a source of new antifungal agents to be explored.


Subject(s)
Animals , Streptococcus mutans , Candida albicans , Virulence Factors , Virulence , Analysis of Variance , Statistics, Nonparametric , Biofilms
18.
Braz. oral res. (Online) ; 35: e062, 2021. tab, graf
Article in English | LILACS, BBO | ID: biblio-1278591

ABSTRACT

Abstract This in vitro study evaluated the impact of TiO2 nanotubes (n-TiO2) incorporated into glass ionomer cement (GIC) on Streptococcus mutans (S. mutans) characteristics at cellular and molecular levels. n-TiO2, synthesized by the alkaline method (20 nm in size), was added to Ketac Molar EasyMix® at 0%, 3%, 5%, and 7% by weight. S. mutans strains were cultured on GIC disks with addition or not of n-TiO2 for 1, 3, and 7 days and the following parameters were assessed: inhibition halo (mm) (n=3/group); cell viability (live/dead) (n=5/group); cell morphology (SEM) (n=3/group); and gene expression by real-time PCR (vicR, covR, gtfB, gtfC, and gtfD) (n=6/group). The data were analyzed by the Kruskal-Wallis test, repeated-measures ANOVA or two-way ANOVA, and Tukey's and Dunn's post-hoc tests (α=0.05). The agar diffusion test showed a higher antibacterial property for 5% n-TiO2 compared with 3% and 7% (p<0.05) with no effect of time (1, 3, and 7 days). The cell number was significantly affected by all n-TiO2 groups, while viability was mostly affected by 3% and 5% n-TiO2, which also affected cell morphology and organization. Real-time PCR demonstrated that n-TiO2 reduced the expression of covR when compared with GIC with no n-TiO2 (p<0.05), with no effect of time, except for 3% n-TiO2 on vicR expression. Within-group and between-group analyses revealed n-TiO2 did not affect mRNA levels of gtfB, gtfC, and gtfD (p>0.05). Incorporation of n-TiO2 at 3% and 5% potentially affected S. mutans viability and the expression of key genes for bacterial survival and growth, improving the anticariogenic properties of GIC.


Subject(s)
Streptococcus mutans , Nanotubes , Titanium , Virulence , Materials Testing , Glass Ionomer Cements/pharmacology
19.
Braz. oral res. (Online) ; 35: e030, 2021. tab
Article in English | LILACS, BBO | ID: biblio-1153618

ABSTRACT

Abstract The present study evaluated the ability of Bifidobacterium and Lactobacillus species associated with streptococci to increase insoluble extracellular polysaccharide (EPS) production and initial caries lesion progression. Bovine enamel blocks (n = 190; 4 mm x 4 mm) were prepared, selected according to initial surface hardness (SH), and divided into two groups: a) double combinations: S. mutans with Bifidobacterium or Lactobacillus, and b) triple combinations: S. mutans and S. sobrinus with Bifidobacterium or Lactobacillus species. The blocks were exposed to the bacterial associations for 7 days. Subsequently, quantity of EPS from biofilms and caries lesion depth were determined by means of colorimetric and cross-sectional enamel hardness (ΔKHN) analysis. The data were submitted to one-way analysis of variance, followed by the Bonferroni test (p < 0.05). S. mutans with B. animalis or B. dentium produced a higher quantity of EPS; S. mutans + B. animalis led to the highest ∆KHN. S. mutans + S. sobrinus + B. longum induced greater EPS and ∆KHN values. In conclusion, associations of B. animalis and B. longum with streptococci promoted EPS production and caries lesion progression.


Subject(s)
Animals , Cattle , Tooth Demineralization , Dental Caries , Streptococcus mutans , Cross-Sectional Studies , Biofilms , Dental Enamel
20.
Rev. cuba. invest. bioméd ; 39(4): e687, oct.-dic. 2020. tab, graf
Article in Spanish | LILACS, CUMED | ID: biblio-1156457

ABSTRACT

Objetivo: Determinar las propiedades antimicrobianas de la incorporación de nanopartículas de óxido de zinc y cobre en un adhesivo de grabado y lavado total sobre Streptococcus mutans en pacientes con restauraciones de resina compuesta confeccionadas con adhesivo cargado. Métodos: Este estudio clínico randomizado, participaron 25 pacientes, de ambos sexos, pertenecientes al posgrado de Ortodoncia de la Facultad de Odontología de la Universidad de Chile, en los cuales se confirmó presencia de Streptococcus mutans en saliva. Se confeccionaron restauraciones de resina compuesta oclusales, en premolares superiores con indicación de exodoncia por el tratamiento de ortodoncia, con adhesivo cargado (cuya composición es 5 / 0.2 por ciento ZnO y Cu respectivamente) y control (sin presencia de nanopartículas en su composición), según el listado de aleatorización. Se tomaron muestras microbiológicas en tres tiempos con la Técnica de la cubeta (antes, 1 semana y 4 semanas posterior a la confección de las restauraciones). Se obtuvieron, aislaron e identificaron colonias de Streptococcus mutans a partir de las muestras obtenidas. Los datos fueron analizados por el test de Mann Whittney. Resultados: El promedio del recuento de UFC (unidades formadoras de colonias) de Streptococcus mutans en el grupo experimental fue mayor posterior a la confección de las restauraciones de resina compuesta.Los resultados de la identificación molecular por PCR demuestran la presencia de Streptococcus mutans en 20 de 25 muestras. Conclusiones: No existen diferencias en el recuento de Streptococcus mutans antes y después de la aplicación del adhesivo sobre las restauraciones de resina compuesta(AU)


Objective: Determine the antimicrobial properties of the incorporation of copper and zinc oxide nanoparticles into a total rinse and etch adhesive against Streptococcus mutans in patients with composite resin restorations made with loaded adhesive. Methods: A randomized clinical study was conducted of 25 patients of both sexes from the orthodontics graduate course taught at the Dental School of the University of Chile, in whom the presence of Streptococcus mutans was confirmed in saliva. Occlusal composite resin restorations were performed in upper premolars with exodontia indicated as part of the orthodontic treatment, using loaded adhesive (composition 5 / 0.2 percent ZnO and Cu, respectively) and control (without nanoparticles in its composition), according to the randomization list. Microbiological samples were taken at three moments applying the tray technique (before, 1 week after and 4 weeks after the restorations). Streptococcus mutans were obtained, isolated and identified from the samples taken. Data analysis was based on the Mann-Whitney test. Results: Mean Streptococcus mutans CFU count in the experimental group was higher after the composite resin restorations were made. Results of PCR molecular identification show the presence of Streptococcus mutans in 20 of 25 samples. Conclusions: No differences were found in the Streptococcus mutans count before and after application of the adhesive over the composite resin restorations(AU)


Subject(s)
Humans , Male , Female , Streptococcus mutans/growth & development , Colony Count, Microbial , Dental Cements/therapeutic use , Metal Nanoparticles/therapeutic use , Surgery, Oral/methods , Epidemiology, Experimental
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