ABSTRACT
OBJECTIVE@#To explore the feasibility and effectiveness of plantar medial thin skin flaps preserving plantar fascia with its superficial fascia tissue to repair skin defects in hands and feet.@*METHODS@#Between July 2017 and January 2023, 35 cases of hand and foot defects were repaired with plantar medial thin skin flaps preserving plantar fascia with its superficial fascia tissue (13 pedicled flaps and 22 free flaps). There were 18 males and 17 females, with an average age of 38.8 years (range, 8-56 years). Thirty cases of defects were caused by trauma, and the interval between injury and admission ranged from 2 to 6 hours (mean, 3.3 hours). Three cases were ulcer wounds with a course of 3.0, 3.8, and 7.0 months, respectively. Two cases were malignant melanoma. Eight cases of wounds located in the fingers, 13 cases in the palm, 12 cases in the heel, and 2 cases in the distal foot. The size of skin defects ranged from 4.0 cm×3.5 cm to 12.0 cm×10.0 cm, and the size of flap ranged from 5.0 cm×4.5 cm to 13.0 cm×11.0 cm. The donor sites were repaired with skin grafts.@*RESULTS@#All flaps were survived and the wounds healed by first intention after operation. The partial necrosis at the edge of the skin graft occurred in 1 case, which healed after dressing change; the other skin grafts survived successfully. All patients were followed up 6-24 months (mean, 18 months). The flaps exhibited similar color and thickness to the surrounding hand and foot skin. Two-point discrimination ranged from 7 to 10 mm in the flaps with an average of 8 mm. The donor sites had no painful scars or sensory abnormalities. Foot and ankle functions were good and gaits were normal.@*CONCLUSION@#Application of plantar medial thin skin flaps preserving plantar fascia with its superficial fascia tissue to repair skin defects in hands and feet had good flap shape, high survival rate of skin graft at the donor site, and no obvious complications.
Subject(s)
Male , Female , Humans , Adult , Plastic Surgery Procedures , Subcutaneous Tissue/surgery , Soft Tissue Injuries/surgery , Skin Transplantation , Fascia , Free Tissue Flaps , Treatment Outcome , Perforator FlapABSTRACT
Realizamos uma análise de quais são os elementos responsáveis pelo sustento e formato abdominal, determinando assim, que é devido a uma excessiva flacidez musculoaponeurótica de origem primária, à qual promove uma incapacidade do suporte da parede abdominal e pode estar relacionada a fatores predisponentes. Para esses casos específicos, desenvolvemos um tratamento propondo a colocação da tela e apresentando nossa experiência. Apresentamos esta série de casos de experiência em 26 anos. Onde 15 pacientes foram tratados com abdominoplastia primária e secundária. O reforço da parede abdominal foi realizado através da colocação de tela de polipropileno no plano submuscular com pontos em U na fáscia transversalis, buscando-se fortalecer o músculo e a fáscia transversa. Os resultados foram satisfatórios a longo prazo. Obtendo resolução das protuberâncias abdominais e restaurando a harmonia dos músculos. Apenas duas complicações ocorreram, que foram a presença de dor crônica localizada no abdome tratada com infiltrações de esteroides e fístula umbilical precoce de resolução rápida espontânea, independente da proposta.
We investigated the causative factors of abdominal support and shape and found that excessive musculoskeletal flaccidity of primary origin causes an inability to support the abdominal wall and may be associated with the predisposing factors. For such cases, we developed a treatment consisting of the placement of a subcutaneous mesh. Here, we present our experience with this treatment. We present a case series of 15 patients in our 26 years of experience who were treated with primary and secondary abdominoplasties. The abdominal wall was reinforced by placing a polypropylene mesh in the submuscular plane with U-stitches in the transversalis fascia, aiming at strengthening the muscle and transverse fascia. The results were satisfactory in the long term. Abdominal bulges were repaired, and muscle harmony was restored. Only two complications occurred: chronic pain localized in the abdomen, which was treated with steroid infiltrations, and an early umbilical fistula with spontaneous and rapid resolution, regardless of the proposal.
Subject(s)
Humans , Female , Adult , Middle Aged , History, 21st Century , Muscular Atrophy , Secondary Treatment , Plastic Surgery Procedures , Abdominal Wall , Subcutaneous Tissue , Abdomen , Superficial Musculoaponeurotic System , Diastasis, Muscle , Muscular Atrophy/surgery , Secondary Treatment/analysis , Secondary Treatment/methods , Plastic Surgery Procedures/methods , Abdominal Wall/anatomy & histology , Subcutaneous Tissue/surgery , Abdominoplasty/methods , Superficial Musculoaponeurotic System/surgery , Diastasis, Muscle/surgery , Abdomen/surgerySubject(s)
Humans , Female , Adult , Carbamazepine/adverse effects , Stevens-Johnson Syndrome/etiology , Subcutaneous Absorption/drug effects , Anticonvulsants/adverse effects , Arm , Biopsy , Carbamazepine/administration & dosage , Stevens-Johnson Syndrome/surgery , Subcutaneous Tissue/surgery , Subcutaneous Tissue/pathology , Anticonvulsants/administration & dosageABSTRACT
ABSTRACT Background: Diastasis of the rectus abdominis muscles (DMRA) is frequent and may be associated with abdominal wall hernias. For patients with redudant skin, dermolipectomy and plication of the diastasis is the most commonly used procedure. However, there is a significant group of patients who do not require skin resection or do not want large incisions. Aim: To describe a "new" technique (subcutaneous onlay laparoscopic approach - SCOLA) for the correction of ventral hernias combined with the DMRA plication and to report the initial results of a case series. Method: SCOLA was applied in 48 patients to correct ventral hernia concomitant to plication of DMRA by pre-aponeurotic endoscopic technique. Results: The mean operative time was 93.5 min. There were no intra-operative complications and no conversion. Seroma was the most frequent complication (n=13, 27%). Only one (2%) had surgical wound infection. After a median follow-up of eight months (2-19), only one (2%) patient presented recurrence of DMRA and one (2%) subcutaneous tissue retraction/fibrosis. Forty-five (93.7%) patients reported being satisfied with outcome. Conclusion: The SCOLA technique is a safe, reproducible and effective alternative for patients with abdominal wall hernia associated with DMRA.
RESUMO Racional: A diástase dos músculos retos abdominais (DMRA) é frequente e pode estar associada à presença de hérnias da parede abdominal. Para pacientes com excesso de pele, a dermolipectomia e plicatura da diástase é o procedimento mais comumente utilizado. Entretanto, há um grupo significativo de pacientes que não necessitam ressecção de pele ou não desejam grandes incisões. Objetivo: Descrever uma "nova" técnica (Subcutaneous Onlay Laparoscopic Approach - SCOLA) para a correção das hérnias ventrais combinada à plicatura da DMRA e relatar os resultados iniciais. Métodos: A técnica SCOLA de correção de hérnia ventral concomitante com a plicatura da DMRA por técnica endoscópica pré-aponeurótica foi aplicada em quarenta e oito pacientes. Resultados: O tempo operatório médio foi de 93,5 min. Não houve nenhuma complicação intra-operatória e nenhuma conversão. Seroma foi a complicação mais frequente (n=13, 27%). Apenas um (2%) apresentou infecção de ferida operatória. Após seguimento médio de oito meses (2-19) apenas um (2%) paciente apresentou recidiva da DMRA e um (2%) retração/fibrose do tecido subcutâneo. Quarenta e cinco (93,7%) relataram estarem satisfeitos com resultado. Conclusão: A técnica SCOLA é alternativa segura, reprodutível e efetiva para pacientes com hérnia da parede abdominal associada à DMRA.
Subject(s)
Humans , Male , Female , Adult , Middle Aged , Laparoscopy/methods , Rectus Abdominis/surgery , Subcutaneous Tissue/surgery , Diastasis, Muscle/surgery , Hernia, Ventral/surgery , Postoperative Complications , Reproducibility of Results , Treatment Outcome , Herniorrhaphy/methods , Operative Time , Diastasis, Muscle/complications , Hernia, Ventral/complications , Intraoperative ComplicationsABSTRACT
ABSTRACT Background: The best site for splenic implant was not defined, mainly evaluating the functionality of the implant. Aim: To evaluate the effects of autogenous splenic implantation on the subcutaneous tissue in the survival of splenectomized rats. Method: Twenty-one randomly assigned rats were studied in three groups (n=7): group 1 - manipulation of the abdominal cavity and preservation of the spleen; group 2 - total splenectomy; group 3 - splenectomy and implant of the tissue removed in the subcutaneous. The animals were followed for 90 days postoperatively. Results: There was a higher mortality in groups 2 (p=0.0072) and 3 (p=0.0172) in relation to group 1. There was no difference between groups 2 and 3 (p=0.9817). Conclusion: The splenic implant in the subcutaneous is ineffective in the survival of rats submitted to splenectomy.
RESUMO Racional: O melhor sítio para implante esplênico não foi definido, principalmente avaliando a funcionalidade do implante. Objetivo: Avaliar os efeitos do implante esplênico autógeno subcutâneo na sobrevida de ratos esplenectomizados. Métodos: Foram estudados 21 ratos alocados aleatoriamente em três grupos (n=7): grupo 1 - manipulação da cavidade abdominal e preservação do baço; grupo 2 - esplenectomia total; grupo 3 - esplenectomia e implante do tecido retirado no subcutâneo. Os animais foram acompanhados por 90 dias pós-operatórios. Resultados: Houve mortalidade maior nos grupos 2 (p=0,0072) e 3 (p=0,0172) em relação ao grupo 1. Não houve diferença entre os grupos 2 e 3 (p=0,9817). Conclusão: O implante esplênico no subcutâneo é ineficaz na sobrevida de ratos submetidos à esplenectomia.
Subject(s)
Animals , Male , Spleen/transplantation , Subcutaneous Tissue/surgery , Splenectomy , Random Allocation , Survival Rate , Organ Transplantation/mortality , Rats, WistarABSTRACT
ABSTRACT Objective: to analyze the chemical components of the smoke from electrocautery from coagulating muscle and liver tissues of pigs. Methods: we collected smoke produced by electrocautery applied to porcine tissue in previously evacuated bottles, with qualitative and quantitative analysis of the compounds present through the hyphenated technique gas chromatography / mass spectrometry. Results: there was a majority of decanal aldehyde in the fumes from the subcutaneous, muscle and liver tissues. Fumes of subcutaneous and muscular tissues also showed the presence of hexanal and phenol. In the fumes of subcutaneous and liver tissues we also found toluene and limonene and, finally, nonanal smoke was present in the muscle and liver tissues. Conclusion: there is increasing evidence showing that smoke from electrocautery used in subcutaneous, muscle and liver tissue is harmful to human health. Thus, there is need to reduce exposure to it or wear masks with filters capable of retaining these particles.
RESUMO Objetivo: analisar quimicamente os componentes da fumaça do eletrocautério, provenientes da coagulação de tecidos, muscular e hepático de suino. Métodos: coleta de fumaça produzida por eletrocauterização de tecido porcino em frascos previamente evacuados com análise qualitativa e quantitativa dos compostos presentes, através de técnica hifenada, cromatografia a gás/espectrometria de massas. Resultados: houve presença majoritária do aldeído decanal nas fumaças provenientes dos tecidos subcutâneo, muscular e hepático. Fumaças dos tecidos subcutâneo e muscular mostraram também a presença de hexanal e fenol. Nas fumaças dos tecidos subcutâneo e hepático foram encontrados ainda tolueno e limoneno e, por fim, nonanal estava presente nas fumaças dos tecidos muscular e hepático. Conclusão: há número crescente de evidências mostrando que fumaça proveniente de eletrocauterização de tecidos subcutâneo, muscular e hepático é nociva à saúde de seres humanos. Portanto, há necessidade de reduzir a exposição a ela ou usar máscara com filtro capaz de reter essas partículas.
Subject(s)
Smoke/analysis , Electrocoagulation , Gas Chromatography-Mass Spectrometry , Swine , Muscle, Skeletal/surgery , Subcutaneous Tissue/surgery , Liver/surgeryABSTRACT
INTRODUÇÃO: Até os anos 70, a reparação de perdas de substância na perna representava, quase sempre, um problema de solução muito difícil ou, até, insolúvel. Atualmente, embora ainda constitua um campo para os mais experientes, as áreas cruentas na perna já contam com várias técnicas confiáveis e algumas relativamente simples para sua reparação. Este trabalho visa equacionar condutas reparadoras de feridas de perna, utilizando tecidos locais. MÉTODO: Estudo retrospectivo pela análise de casos de reconstrução de perna com retalhos locais realizados pelos autores. Foram incluídos os retalhos dermoadiposos, fasciocutâneos, fasciossubcutâneos e musculares. RESULTADOS: Foram operados 70 pacientes que possuíam áreas cruentas na perna, em consequência de fratura de tíbia, osteomielite, perda tecidual isquêmica, úlcera crônica e tumoração de pele. Os resultados foram avaliados segundo etiologia, tipo de procedimento cirúrgico e complicações. CONCLUSÕES: A opção do tratamento de áreas cruentas de membros inferiores com retalhos locais é bastante válida. A escolha do retalho vai depender de condições locais da perna e da região anatômica afetada. No terço superior da perna, utilizamos retalhos fasciocutâneos baseados na rede vascular do joelho ou retalho de gastrocnêmio. Já no médio, os principais retalhos foram o solear e o fasciossubcutâneo de panturrilha. E, por fim, no inferior, o principal retalho usado foi o fasciossubcutâneo de panturrilha.
INTRODUCTION: Until the 70s, repairing loss of tissue in the leg was almost always difficult, or even impossible. Currently, only the most experienced surgeons are able to repair open wounds of the leg. Nevertheless, several reliable and simple techniques are currently available. This work aimed to evaluate repair techniques for leg wounds by using local tissues. METHOD: The authors performed a retrospective study of cases of leg reconstruction using local flaps. Dermoadipose, fasciocutaneous, fasciosubcutaneous, and muscle flaps were used. RESULTS: Seventy patients who had open areas in the leg due to tibial fractures, osteomyelitis, ischemic tissue loss, chronic ulcer, or skin tumor underwent surgery. Results were evaluated according to etiology, type of surgical procedure, and complications. CONCLUSIONS: The option of treating open wounds of the lower limbs by using local flaps is very valid. Selection of the flap type depended on local conditions in the leg, and the anatomical region affected. In the upper third of the leg, we used fasciocutaneous flaps, based on the vascular network of the knee, or gastrocnemius flaps. In the middle third of the leg, we used the soleus muscle as the primary flap, and fasciosubcutaneous flaps in the calf region. In the lower third of the leg, a fasciosubcutaneous flap of the calf was primarily used.
Subject(s)
Humans , Female , Adult , History, 21st Century , Wounds and Injuries , Medical Records , Retrospective Studies , Muscle, Skeletal , Plastic Surgery Procedures , Evaluation Study , Lower Extremity , Subcutaneous Tissue , Perforator Flap , Leg , Wounds and Injuries/surgery , Wounds and Injuries/therapy , Medical Records/standards , Muscle, Skeletal/surgery , Muscle, Skeletal/pathology , Plastic Surgery Procedures/methods , Lower Extremity/surgery , Subcutaneous Tissue/anatomy & histology , Subcutaneous Tissue/surgery , Subcutaneous Tissue/injuries , Perforator Flap/surgery , Leg/surgery , Leg/pathologyABSTRACT
A 25-year-old woman was seen for a painless subcutaneous mass of 2 years duration. On excisional biopsy, a collapsed cystic structure lined by stratified, ciliated, columnar epithelium was noted. These linning cells did not produce mucin. Immunohistochemical staining for progesterone receptor, estrogen receptor and epithelial membrane antigen was positive, whereas it was negative for carcinoembryonic antigen. Findings were consistent with cutaneous ciliated cyst (CCC). CCCs are rare, predominantly occurring on the lower extremities of young women. Most of them have been regarded as Mullerian remnants. A case of a CCC in the subcutaneous area is reported.
Subject(s)
Adult , Biopsy , Cysts/diagnosis , Cysts/pathology , Cysts/surgery , Female , Histocytochemistry , Humans , Immunohistochemistry , Microscopy , Mucin-1/analysis , Receptors, Estrogen/analysis , Receptors, Progesterone/analysis , Subcutaneous Tissue/pathology , Subcutaneous Tissue/surgeryABSTRACT
The aim of this study was to morphometrically analyze the tissue response to a customized pin obtained from devitalized bovine cortical bone (DBCB-pin) implanted in the subcutaneous tissue of rats, as well as to assess its microstructural aspect by scanning electron microscopy (SEM). The pins were implanted in the subcutaneous tissue of 20 rats, which were killed at 7, 14, 28 and 60 days (5 rats/period) after implantation. In the subcutaneous tissue, DBCB-pin promoted the formation of a fibrous capsule. At 7 days, capsule showed thickness of 70 ± 3.2 µm with higher density of newly formed capillaries and smaller density of collagen fibers. Between 14 and 60 days, more organized fibrous capsule exhibited smaller thickness (53 ± 5.5 µm) with higher density of fibroblasts and collagen fibers. In this period, a small and slow bioresorption of the DBCB-pin by macrophages and rare multinucleated giant cells without tissue damage was observed. The thickness of DBCB-pin resorbed was in mean only of 9.3 µm. During all experimental periods not occurred presence of immune reaction cells as lymphocytes and plasma cells. It was concluded that the pin derived from cortical bovine bone was well tolerated by subcutaneous tissue of rats and slowly resorbed could be an alternative material for membrane fixation in the guided tissue regeneration procedures.
O objetivo deste estudo foi analisar morfometricamente a resposta tecidual a um pino obtido a partir de osso bovino desvitalizado cortical (DBCB pinos) implantado no tecido subcutâneo de ratos, bem como para avaliar o seu aspecto microestrutural por microscopia eletrônica de varredura (MEV). Os pinos foram implantados no tecido subcutâneo de 20 ratos, que foram sacrificados aos 7, 14, 28 e 60 dias (5 animais / período) após a implantação. No tecido subcutâneo, o pino DBCB promoveu a formação de uma cápsula fibrosa. Aos 7 dias, a cápsula apresentou espessura de 70 ± 3,2 μm com maior densidade de capilares neoformados e menor densidade de fibras colágenas. Entre 14 e 60 dias, a cápsula fibrosa apresentava-se mais organizada e exibiram menor espessura (53 ± 5,5 μm) com maior densidade de fibroblastos e fibras colágenas. Nesse período, foi observada uma bioreabsorção pequena e lenta dos pinos DBCB por macrófagos e raras células gigantes multinucleadas, sem dano tecidual. A espessura dos pinos DBCB reabsorvidos foi em média de apenas 9,3 µm. Durante todos os períodos experimentais não ocorreu presença de células como linfócitos e células plasmáticas. Concluiu-se que o pino derivado de osso bovino cortical foi bem tolerado pelo tecido subcutâneo de ratos e reabsorvido lentamente, sendo um potencial material alternativo para fixação da membrana nos procedimentos de regeneração tecidual guiada.
Subject(s)
Animals , Cattle , Male , Rats , Absorbable Implants , Biocompatible Materials , Dental Pins , Guided Tissue Regeneration, Periodontal/instrumentation , Bone and Bones , Implants, Experimental , Membranes, Artificial , Rats, Wistar , Subcutaneous Tissue/surgeryABSTRACT
FUNDAMENTOS: A quitosana é polímero derivado da quitina, com vários tipos de aplicação na área médica. OBJETIVO: Avaliar a biocompatibilidade de membranas de quitosana no subcutâneo de ratos. MÉTODOS: Foram utilizados 20 ratos "Wistar" machos, nos quais foram implantadas membranas de quitosana, na região mediana dorsal. Os animais foram sacrificados: sete, 15, 30 e 60 dias após a cirurgia, tendo sido avaliados clinicamente durante o período experimental e com fotodocumentação no momento do sacrifício. Após o sacrifício, as membranas e tecidos adjacentes foram removidos e preparados para exame histológico e morfométrico. RESULTADOS: Nenhum animal apresentou efeitos adversos que pudessem ser atribuídos à implantação das membranas. O exame histológico mostrou que as inclusões são lisas e homogêneas e não são colonizadas por células do hospedeiro, sendo circundadas por pseudocápsula composta por fibroblastos e células inflamatórias. A morfometria da pseudocápsula revelou espessura semelhante durante todo o período experimental (P>0,05). CONCLUSÃO: A quitosana pode ser opção para uso como implante não integrado. Novos estudos devem ser realizados para comprovar a biocompatibilidade a longo prazo.
Background: Chitosan is a polymer derivative from chitin applied in many medical specialties. Objective: To evaluate the biocompatibility of chitosan membranes used as inclusion material into subcutaneous of rats. Methods: Twenty male Wistar rats received chitosan membranes into the subcutaneous area of the dorsal medial region. The animals were randomly divided in four groups with 5 animals each, sacrificed on 7th (G1), 15th (G2), 30th (G3) and 60th (G4) postoperative day (PO). All animals were clinically evaluated daily and by photo-documentation at the sacrifice moment. The animals and the material were assessed for evidence of host response by histological and morphometric evaluation of the implants and the surrounding soft tissues. The material was fixed in 10% formalin and then the sections were stained with hematoxylin and eosin. Results: None of animals presented side effects attributable to the implants. The histological evaluation showed smooth and homogeneous inclusions with no host cells inside and were encircled by a pseudocapsule of fibroblasts and inflammatory cells. The morphometric evaluation showed no statistical difference between different groups (P>0.05). Conclusion: Chitosan membrane might be suggested as an option of non-integrated implant. Otherstudies have to be carried out to evaluate longstanding biocompatibility.
Subject(s)
Animals , Male , Rats , Chitosan , Materials Testing , Prostheses and Implants , Subcutaneous Tissue , Rats, Wistar , Subcutaneous Tissue/anatomy & histology , Subcutaneous Tissue/surgeryABSTRACT
Introdução: Os autores propõem uma nova técnica de suspensão de nádegas e coxas em pacientes pós-grandes emagrecimentos, sobrepondo o volume das nádegas, elevando-as e projetando-as. Método: A região trocantérica e a face interna das coxas, quando necessário, são lipoaspiradas.Retira-se o excesso de pele por incisão que parte do sulco interglúteo, contorna a crista ilíaca posterior e anterior, desce pela prega inguinal até a face interna da coxa e, em L, desce até acima dos joelhos. As ressecções de pele são feitas sempre acima da fascia superficialis. Inicia-se a cirurgia em decúbitoventral para a suspensão das nádegas e conclui-se em decúbito dorsal com a suspensão das coxas.
Introduction: The authors propose a new technique of buttocks and thighs lift in massive weigh loss patients, overlapping the volume of the buttocks, elevating and projecting them. Methods:The trocantheric area and the internal aspect of the thighs, when necessary, are liposuctioned. They remove the skin excess through an incision that initiates in the intergluteal furrow and outlines the posterior and anterior iliac crest. It goes down for the pleat of the groins until the internal face of the thigh and, in L, it goes down near the knees. The skin resections are always done above the fascia superficialis. The surgery begins in ventral decubitus for the suspension of the buttocks and it is completed in dorsal decubitus with the suspension of the thighs.
Subject(s)
Humans , Adult , Female , Cicatrix, Hypertrophic , Surgery, Plastic/methods , Lower Extremity/surgery , Intraoperative Complications , Surgical Flaps , Subcutaneous Tissue/surgery , Hip/surgery , Methods , Buttocks/surgery , Surgical Procedures, Operative , Diagnostic Techniques and ProceduresABSTRACT
Wound disruption after cesarean section is a common complication; and obesity has been identified as strong independent risk factor for wound complications. The aim of this study was to test the hypothesis that closure of the subcutaneous fat decreases the incidence of wound infection and disruption after cesarean delivery. In a clinical trial study conducted in Yazd, a hundred women with at least 2 cm of subcutaneous fat and cesarean delivery were included in the study. In 50 patients, subcutaneous tissue was closed using synthetic suture [group A], while in 50 control patients subcutaneous tissue was not closed [group B]. Two groups were compare in relation to wound infection and disruption. Data were analyzed using student and X[2] tests. Complications leading to wound infections [positive culture] were 2 women in group A and 5 women in group B [P<0.05] and disruption or opening of the incision were in 7 women in group A and in 17 women in group B [P<0.01]. Closure of the subcutaneous tissue can significantly reduce the rate of postoperative wound disruption in women with at least 2 cm of subcutaneous adipose tissue
Subject(s)
Humans , Female , Cesarean Section , Subcutaneous Tissue/surgery , Risk Factors , Treatment OutcomeABSTRACT
The ideal bone graft must present biocompatibility, osteoconductive and osteoinductive properties, resistance and plasticity. Xenogenic grafts of bovine cancellous bone origin are particularly interesting due to their biologically designed porous structure that enhance both cellular and vascular invasion. The purpose of this study was to evaluate the tissue response induced by bovine macrogranular porous anorganic bone implanted in rat subcutaneous tissue. Forty rats were assigned to 2 groups, as follows: the control group received empty collagen capsules and the test group received subcutaneous implants of the test material. Samples were collected after 10, 20, 30 and 60 days and processed histologically. Histological analysis showed at 10 days a granulomatous inflammatory infiltrate, rich in multinucleated giant cells and free of lymphocytes or plasma cells, similarly to mineralized allograft implanted in rat subcutaneous. In later periods, there was a significant decrease in the inflammatory infiltrate and an increase in fibrosis around graft particles. In conclusion, the test material induced a foreign body-type granuloma with subsequent fibrosis around the graft particles implanted in rat subcutaneous and did not elicit any immune response, thus being considered biocompatible.
O enxerto ósseo ideal deve possuir características como biocompatibilidade, capacidade osteocondutora, osteoindutora, resistência e plasticidade. Dentre os implantes xenogênicos de origem bovina, os produzidos com o osso esponjoso revestem-se de particular interesse devido a sua arquitetura constituída de poros biologicamente desenhados que favorecem a invasão celular e vascular até o centro do defeito. O objetivo deste estudo foi avaliar a resposta tecidual ao material de osso inorgânico medular bovino macrogranular. Quarenta ratos foram divididos em 2 grupos (n=20): o grupo controle recebeu cápsulas de colágeno vazias, e o grupo experimental recebeu implante subcutâneo do material teste. As amostras foram coletadas após 10, 20, 30 e 60 dias de implantação e processadas histotecnicamente. A análise histológica mostrou aos 10 dias pós-cirúrgicos que o infiltrado inflamatório era do tipo granulomatoso rico em células gigantes multinucleadas, mas livre de linfócitos ou plasmócitos, quadro similar ao observado para aloenxertos mineralizados implantados em subcutâneo de ratos. Com o avançar do tempo experimental houve significante diminuição do infiltrado inflamatório inicial concomitantemente ao aumento no grau de fibrosamento ao redor das partículas implantadas. Concluiu-se que o material de enxerto testado em tecido conjuntivo subcutâneo de ratos induziu um granuloma tipo corpo estranho e fibrose ao redor das partículas implantadas, resposta semelhante do mesmo tecido aos aloenxertos mineralizados, e não desencadeou nenhuma resposta imune, sendo portanto biocompatível.