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1.
Braz. j. med. biol. res ; 47(12): 1050-1056, 12/2014. graf
Article in English | LILACS | ID: lil-727667

ABSTRACT

People who suffer from traumatic brain injury (TBI) often experience cognitive deficits in spatial reference and working memory. The possible roles of cyclooxygenase-1 (COX-1) in learning and memory impairment in mice with TBI are far from well known. Adult mice subjected to TBI were treated with the COX-1 selective inhibitor SC560. Performance in the open field and on the beam walk was then used to assess motor and behavioral function 1, 3, 7, 14, and 21 days following injury. Acquisition of spatial learning and memory retention was assessed using the Morris water maze on day 15 post-TBI. The expressions of COX-1, prostaglandin E2 (PGE2), interleukin (IL)-6, brain-derived neurotrophic factor (BDNF), platelet-derived growth factor BB (PDGF-BB), synapsin-I, and synaptophysin were detected in TBI mice. Administration of SC560 improved performance of beam walk tasks as well as spatial learning and memory after TBI. SC560 also reduced expressions of inflammatory markers IL-6 and PGE2, and reversed the expressions of COX-1, BDNF, PDGF-BB, synapsin-I, and synaptophysin in TBI mice. The present findings demonstrated that COX-1 might play an important role in cognitive deficits after TBI and that selective COX-1 inhibition should be further investigated as a potential therapeutic approach for TBI.


Subject(s)
Animals , Brain Injuries/complications , Cerebral Cortex/injuries , Cyclooxygenase 1/physiology , Cyclooxygenase Inhibitors/therapeutic use , Learning/drug effects , Memory Disorders/drug therapy , Pyrazoles/therapeutic use , Blotting, Western , Brain-Derived Neurotrophic Factor/metabolism , Cerebral Decortication , Cyclooxygenase 1/metabolism , Disease Models, Animal , Dinoprostone/analysis , Dinoprostone/metabolism , Enzyme-Linked Immunosorbent Assay , Hippocampus/metabolism , /blood , Maze Learning/drug effects , Memory Disorders/etiology , Memory Disorders/metabolism , Proto-Oncogene Proteins c-sis/metabolism , Recovery of Function/drug effects , Synaptophysin/analysis , Synaptophysin/metabolism
2.
Article in English | WPRIM | ID: wpr-62194

ABSTRACT

Gangliocytic paraganglioma (GP) is a rare, benign tumor which is usually found in the duodenum. We here report four recent cases of GP, with successful endoscopic resection in three cases, including a lesion on the ampulla of Vater. In all cases, each lesion had a stalk that facilitated removal using an endoscopic approach. Endoscopic mucosal resection is a feasible and safe treatment if the location, depth, and lymph node status are all favorable and is also helpful for definite diagnosis of unknown duodenal mass. To avoid morbidity resulting from open surgical resection, careful inspection for the peduncle of the GP will help determine the feasibility of endoscopic resection.


Subject(s)
Aged , Ampulla of Vater/pathology , Chromogranin A/metabolism , Colonoscopy , Duodenal Neoplasms/pathology , Endoscopy, Gastrointestinal , Female , Humans , Immunohistochemistry , Intestinal Mucosa/pathology , Male , Middle Aged , Neuroendocrine Tumors/pathology , Paraganglioma/pathology , S100 Proteins/metabolism , Synaptophysin/metabolism , Tomography, X-Ray Computed
3.
Article in Korean | WPRIM | ID: wpr-169070

ABSTRACT

Basaloid squamous cell carcinoma is a rare and aggressive variant of squamous cell carcinoma, which mostly occurs in the upper aerodigestive tracts. Basaloid squamous cell carcinoma also typically arises in the anal canal, but is extremely rare in the lower gastrointestinal tract. A 70-year-old man presented with loose stool and intermittent hematochezia 2 months ago. Colonoscopy showed an ulceroinfiltrative mass on the rectosigmoid colon from 16 cm to 18 cm above the anal verge. Conventional colonoscope could not pass through the lesion but it was possible with pediatric colonoscope. Abdominal CT scan showed 1.6 cm sized wall thickening with circumferential luminal narrowing in the rectosigmoid colon and multiple ill-defined low density masses in both lobes of the liver. Therefore, colon cancer with liver metastasis was suspected. However, basaloid cells were noted on histologic examination, and they were weakly positive for synaptophysin on immunohistochemical study. After palliative lower anterior resection, histologic examination of the resected specimen revealed basaloid differentiation with keratin pearls, and tumor cells were positively stained with high molecular weighted cytokeratin (34BE12) and CK 5/6. Thus, the patient was finally diagnosed with basaloid squamous cell carcinoma of rectosigmoid colon with distant metastases.


Subject(s)
Aged , Carcinoma, Squamous Cell/diagnosis , Colonoscopy , Colorectal Neoplasms/diagnosis , Humans , Immunohistochemistry , Keratins/metabolism , Liver Neoplasms/diagnostic imaging , Lung Neoplasms/diagnostic imaging , Male , Positron-Emission Tomography , Synaptophysin/metabolism , Tomography, X-Ray Computed
4.
Article in Korean | WPRIM | ID: wpr-143721

ABSTRACT

Gastrointestinal neuroendocrine tumors arise from cells of the diffuse neuroendocrine system and can take place almost anywhere within the gastrointestinal tract. A 40-year-old man admitted to evaluate a duodenal subepithelial lesion which was incidentally found at health check-up. The polypoid duodenal subepithelial lesion, measuring about 7 mm, was removed by the endoscopic mucosal resection and the pathology confirmed a neuroendocrine tumor. Abdominopelvic computed tomography, done for staging work up, revealed a mass in the pancreatic head and the patient received pylorus preserving pancreaticoduodenectomy. Mass at the pancreas also found out to be neuroendocrine tumor but showed different histopathologic traits under immunohistochemical staining. The patient was also diagnosed as hyperparathyroidism and pituitary microadenoma. Finally, multiple endocrine neoplasia type 1 was confirmed, which was accompanied by duodenal neuroendocrine tumor.


Subject(s)
Adult , CD56 Antigen/metabolism , Duodenum/pathology , Endoscopy, Digestive System , Humans , Immunohistochemistry , Magnetic Resonance Imaging , Male , Neoplasms, Multiple Primary , Neuroendocrine Tumors/diagnosis , Pancreas/pathology , Synaptophysin/metabolism , Tomography, X-Ray Computed
5.
Article in Korean | WPRIM | ID: wpr-143712

ABSTRACT

Gastrointestinal neuroendocrine tumors arise from cells of the diffuse neuroendocrine system and can take place almost anywhere within the gastrointestinal tract. A 40-year-old man admitted to evaluate a duodenal subepithelial lesion which was incidentally found at health check-up. The polypoid duodenal subepithelial lesion, measuring about 7 mm, was removed by the endoscopic mucosal resection and the pathology confirmed a neuroendocrine tumor. Abdominopelvic computed tomography, done for staging work up, revealed a mass in the pancreatic head and the patient received pylorus preserving pancreaticoduodenectomy. Mass at the pancreas also found out to be neuroendocrine tumor but showed different histopathologic traits under immunohistochemical staining. The patient was also diagnosed as hyperparathyroidism and pituitary microadenoma. Finally, multiple endocrine neoplasia type 1 was confirmed, which was accompanied by duodenal neuroendocrine tumor.


Subject(s)
Adult , CD56 Antigen/metabolism , Duodenum/pathology , Endoscopy, Digestive System , Humans , Immunohistochemistry , Magnetic Resonance Imaging , Male , Neoplasms, Multiple Primary , Neuroendocrine Tumors/diagnosis , Pancreas/pathology , Synaptophysin/metabolism , Tomography, X-Ray Computed
6.
Article in Korean | WPRIM | ID: wpr-11954

ABSTRACT

Collision tumors of the colon are rare. A 54-year-old man was referred to our hospital for the evaluation of hematochezia. Colonoscopy demonstrated the presence of about 3 cm sized mass in the rectosigmoid junction. After surgical resection, the colonic lesion was histologically composed of two discrete lesions: adenocarcinoma in the superficial layer and poorly differentiated neuroendocrine carcinoma in the deeper layer. We report this case of colonic collision tumor (adenocarcinoma and neuroendocrine carcinoma) with a review of the literature.


Subject(s)
Adenocarcinoma/diagnosis , Antigens, CD/metabolism , Antigens, Differentiation, T-Lymphocyte/metabolism , Carcinoma, Neuroendocrine/diagnosis , Colonic Neoplasms/diagnosis , Colonoscopy , Humans , Male , Middle Aged , Positron Emission Tomography Computed Tomography , Synaptophysin/metabolism , Tomography, X-Ray Computed
8.
Article in English | WPRIM | ID: wpr-162255

ABSTRACT

During membrane depolarization associated with skeletal excitation-contraction (EC) coupling, dihydropyridine receptor [DHPR, a L-type Ca2+ channel in the transverse (t)-tubule membrane] undergoes conformational changes that are transmitted to ryanodine receptor 1 [RyR1, an internal Ca2+-release channel in the sarcoplasmic reticulum (SR) membrane] causing Ca2+ release from the SR. Canonical-type transient receptor potential cation channel 3 (TRPC3), an extracellular Ca2+-entry channel in the t-tubule and plasma membrane, is required for full-gain of skeletal EC coupling. To examine additional role(s) for TRPC3 in skeletal muscle other than mediation of EC coupling, in the present study, we created a stable myoblast line with reduced TRPC3 expression and without alpha1SDHPR (MDG/TRPC3 KD myoblast) by knock-down of TRPC3 in alpha1SDHPR-null muscular dysgenic (MDG) myoblasts using retrovirus-delivered small interference RNAs in order to eliminate any DHPR-associated EC coupling-related events. Unlike wild-type or alpha1SDHPR-null MDG myoblasts, MDG/TRPC3 KD myoblasts exhibited dramatic changes in cellular morphology (e.g., unusual expansion of both cell volume and the plasma membrane, and multi-nuclei) and failed to differentiate into myotubes possibly due to increased Ca2+ content in the SR. These results suggest that TRPC3 plays an important role in the maintenance of skeletal muscle myoblasts and myotubes.


Subject(s)
Animals , Calcium/metabolism , Calcium Channels/metabolism , Calcium Channels, L-Type/genetics , Cations/metabolism , Cell Differentiation , Cell Proliferation , Cells, Cultured , Excitation Contraction Coupling , Gene Knockdown Techniques , Membrane Potentials , Mice , Muscle Fibers, Skeletal/metabolism , Muscle Proteins/metabolism , Myoblasts, Skeletal/metabolism , Ryanodine Receptor Calcium Release Channel/metabolism , Sarcoplasmic Reticulum/physiology , Synaptophysin/metabolism , TRPC Cation Channels/genetics , Transient Receptor Potential Channels/metabolism
9.
Article in Korean | WPRIM | ID: wpr-37306

ABSTRACT

Pancreas acinar cell carcinoma (ACC) accounts for only 1-2% of pancreatic exocrine malignant tumor. The symptoms of patients with ACC are usually non-specific, for example the anorexia and weight loss. Patients may develop Schmid's triad including subcutaneous fat necrosis, polyarthritis, and eosinophilia. We reported a case of ACC which was manifested by subcutaneous nodule as initial clinical symptom. To our knowledge, this is the first reported case of ACC presenting as subcutaneous fat necrosis in Korea.


Subject(s)
Carcinoma, Acinar Cell/diagnosis , Fat Necrosis/pathology , Humans , Keratins/metabolism , Male , Middle Aged , Pancreatic Neoplasms/diagnosis , Subcutaneous Fat/pathology , Synaptophysin/metabolism , Tomography, X-Ray Computed
10.
Article in English | WPRIM | ID: wpr-44277

ABSTRACT

The aim of this study was to evaluate the effect of early motor balance and coordination training on functional recovery and brain plasticity in an ischemic rat stroke model, compared with simple locomotor exercise. Adult male Sprague-Dawley rats with cortical infarcts were trained under one of four conditions: nontrained control, treadmill training, motor training on the Rota-rod, or both Rota-rod and treadmill training. All types of training were performed from post-operation day 1 to 14. Neurological and behavioral performance was evaluated by Menzies' scale, the prehensile test, and the limb placement test, at post-operation day 1, 7, and 14. Both Rota-rod and treadmill training increased the expression of synaptophysin in subcortical regions of the ischemic hemisphere including the hippocampus, dentate gyrus, and thalamus, but did not affect levels of brain-derived neurotrophic factor or tyrosin kinase receptor B. The Rota-rod training also improved Menzies' scale and limb placement test scores, whereas the simple treadmill training did neither. The control group showed significant change only in Menzies' scale score. This study suggests that early motor balance and coordination training may induce plastic changes in subcortical regions of the ischemic hemisphere after stroke accompanied with the recovery of sensorimotor performance.


Subject(s)
Animals , Brain Ischemia/metabolism , Brain-Derived Neurotrophic Factor/metabolism , Dentate Gyrus/metabolism , Disease Models, Animal , Hippocampus/metabolism , Immunohistochemistry , Male , Motor Activity , Neuronal Plasticity/physiology , Physical Conditioning, Animal , Physical Therapy Modalities , Rats , Rats, Sprague-Dawley , Receptor, trkB/metabolism , Stroke/metabolism , Synaptophysin/metabolism , Thalamus/metabolism , Time Factors
11.
Article in Korean | WPRIM | ID: wpr-60794

ABSTRACT

Paraganglioma is a rare neuroendocrine tumor arising from the neural crest, which includes tissues such as the adrenal medulla, carotid and aortic body, organs of Zuckerkandl, and other unnamed paraganglia. The head, neck, and retroperitoneum are the most common sites for paraganglioma. However, paraganglioma of the pancreas is extremely rare. We report our experience of this rare disease. A 70-year old female patient admitted for a pancreas tail mass detected by computed tomography (CT) scan checked for vague left flank pain. CT with contrast enhancement showed a 4.2-cm heterogeneously enhanced lesion in the tail of the pancreas. A well defined ovoid shape mass in left adrenal gland was suggested adenoma. Distal pancreatectomy with left adrenalectomy was performed. Any lymph node enlargement was not found. Pancreas mass did not invade adjacent organ. Microscopic examination with pancreas and adrenal gland revealed that the cells were arranged in a characteristic Zellballen pattern. Immunohistochemical staining revealed positivity for neuron-specific enolase, chromogranin A, synaptophysin, and S-100 protein. On the basis of these findings, we diagnosed the tumor as a paraganglioma of the pancreas and adrenal gland. We report the first case of pancreas paraganglioma in Korea.


Subject(s)
Adenoma/diagnosis , Adrenal Gland Neoplasms/diagnosis , Aged , Chromogranin A/metabolism , Female , Humans , Pancreatic Neoplasms/diagnosis , Paraganglioma/diagnosis , Phosphopyruvate Hydratase/metabolism , S100 Proteins/metabolism , Synaptophysin/metabolism , Tomography, X-Ray Computed
12.
Article in English | WPRIM | ID: wpr-19813

ABSTRACT

We report a case of small cell carcinoma of extrahepatic bile duct presenting with jaundice and hemobilia. A 59-year-old woman was admitted due to right upper quadrant pain and jaundice. An abdominal computed tomography revealed a 2 cm sized mass in the extrahepatic bile duct. Endoscopic retrograde cholangiopancreatography revealed bloody discharge coming out of the papillary orifice in endoscopic view and a dilated extrahepatic bile duct with multiple irregular filling defects in cholangiogram. A coronal T2-weighted image revealed a hyperintense mass at extrahepatic bile duct. Laparotomy was performed, and pathologic examination of resected specimen showed tumor cells having round to oval nuclei with coarsely granular chromatin and scanty cytoplasm, which were immunoreactive for synaptophysin and chromogranin A, compatible with the diagnosis of small cell carcinoma. The small cell carcinoma of bile duct, despite its rarity, should be considered in differential diagnosis of the causes for obstructive jaundice and hemobilia.


Subject(s)
Bile Duct Neoplasms/diagnosis , Bile Ducts, Extrahepatic/pathology , Carcinoma, Small Cell/diagnosis , Cholangiopancreatography, Endoscopic Retrograde , Chromogranin A/metabolism , Female , Hemobilia/complications , Humans , Magnetic Resonance Imaging , Middle Aged , Synaptophysin/metabolism , Tomography, X-Ray Computed
13.
Arq. neuropsiquiatr ; 66(2b): 385-390, jun. 2008. graf, tab
Article in English | LILACS | ID: lil-486196

ABSTRACT

Medulloblastoma (MB) is the most common malignant brain tumor in childhood. The alterations found include: presence of oncoproteins p53 and HER2, elevated mitotic index, and presence of neuronal differentiation. The aim of this study was to determine the immunohistochemical expression of markers Ki-67, NeuN, synaptophysin, HER2 and p53 in 40 MB samples and their correlation with clinicopathologic parameters and survival. In 29 patients (72.5 percent), >20 percent of cells were positive for Ki-67. Males showed greater ki-67 expression (p=0.02) and smaller survival rates (p=0.002). NeuN and synaptophysin were negative in 16 (40 percent) and 8 (20 percent) cases, respectively. P53 was positive in 18 (45 percent) cases, with 11 (61 percent) weakly positive and 7 (39 percent) strongly positive. HER2 was positive in 23 (57.5 percent) of the samples and did not show statistical association with survival (p=0.07).


Meduloblastoma (MB) é o tumor maligno encefálico mais freqüente na infância. dentre as alterações encontradas estão: a presença das oncoproteínas p53 e HER2, elevado índice mitótico e presença de diferenciação neuronal. o objetivo deste estudo foi determinar a expressão imunoistoquímica (IMQ) dos marcadores Ki-67, NeuN, sinaptofisina, HER2 e p53 em 40 amostras de MB, correlacionando-as com parâmetros clinicopatológicos e com a sobrevida. Vinte e nove pacientes (72,5 por cento) apresentaram 20 por cento ou mais das células positivas para Ki-67. os pacientes do sexo masculino apresentaram maior expressão do Ki-67 (p=0,02) e também menor sobrevida (p=0,002). NeuN e sinaptofisina foram negativos em 16 (40 por cento) e 8 (20 por cento) casos, respectivamente. P53 foi positivo em 18 (45 por cento) casos, sendo 11 (61 por cento) fracamente positivos e 7 (39 por cento) fortemente positivos. HER2 foi positivo em 23 (57,5 por cento) das amostras e não demonstrou associação estatística com a sobrevida (p=0.07).


Subject(s)
Adolescent , Child , Child, Preschool , Female , Humans , Infant , Male , Cerebellar Neoplasms/pathology , Medulloblastoma/pathology , Biomarkers, Tumor/metabolism , Antigens, Nuclear/metabolism , Brazil/epidemiology , Cerebellar Neoplasms/metabolism , Cerebellar Neoplasms/mortality , Epidemiologic Methods , /metabolism , Medulloblastoma/metabolism , Medulloblastoma/mortality , Neoplasm, Residual , Nerve Tissue Proteins/metabolism , /metabolism , Synaptophysin/metabolism , /metabolism
14.
Indian J Pathol Microbiol ; 2008 Apr-Jun; 51(2): 237-9
Article in English | IMSEAR | ID: sea-73814

ABSTRACT

Adrenocortical oncocytoma is very rare. Less than five functioning types of them are reported and most of the reported cases are incidentally found. We herein report a case of functioning adrenocortical oncocytoma of the left adrenal cortex in a young woman.


Subject(s)
Adenoma, Oxyphilic/complications , Adrenal Cortex Neoplasms/complications , Adult , Cushing Syndrome/etiology , Female , Humans , Immunohistochemistry , Synaptophysin/metabolism , Vimentin/metabolism
15.
Indian J Pathol Microbiol ; 2006 Oct; 49(4): 543-5
Article in English | IMSEAR | ID: sea-74326

ABSTRACT

Central neurocytomas are benign neuronal tumours generally found in the lateral or third ventricles. They are rare, comprising < 1% of all brain tumours. It is frequently confused with other tumours of the central nervous system particularly oligodendroglioma. The present study was done to analyse the histopathological features including immunohistochemical profile of these rare tumours. Eight cases were taken up for the study. Seven of the cases had an intraventricular location and one was located outside the ventricles. Increased intracranial pressure was the most common presenting symptom. Microscopically all tumours were composed of small uniform cells with perinuclear halos and regular round nuclei. The tumour in extraventricular location showed atypical features. Immunohistochemistry showed positivity for neuronal markers. The present series highlights the characteristic clinical and pathological findings of this rare brain tumour. Immunostaining for neuronal markers are essential for distinguishing them from other small round cell tumours of the brain.


Subject(s)
Adolescent , Adult , Cerebral Ventricle Neoplasms/pathology , Child, Preschool , Female , Humans , Immunohistochemistry , Intracranial Hypertension , Male , Nerve Tissue Proteins/metabolism , Neurocytoma/pathology , Neurons/metabolism , Synaptophysin/metabolism
16.
Indian J Pathol Microbiol ; 2005 Jan; 48(1): 29-31
Article in English | IMSEAR | ID: sea-72838

ABSTRACT

Cerebellar liponeurocytomas have been included in the 2000 classification of tumours of the central nervous system, under the heading of glioneuronal tumours. The tumour has two populations of cells- one composed of cells with morphology of neurocytes and the other are lipidised cells which look like mature fat cells. The tumour occurs in adults and has a good prognosis. Less than fifteen cases have been reported in world literature. We report a case of this rare tumour entity in a 32 years old female patient.


Subject(s)
Adult , Cerebellar Neoplasms/diagnosis , Female , Glial Fibrillary Acidic Protein/metabolism , Humans , Immunohistochemistry , Lipoma/diagnosis , Neurocytoma/diagnosis , Synaptophysin/metabolism
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