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1.
Arq. bras. med. vet. zootec. (Online) ; 73(4): 763-770, Jul.-Aug. 2021. tab, ilus
Article in English | ID: biblio-1285283

ABSTRACT

The objective of this study was to determine the scrotal thermographic profile and to verify the influence of temperature and humidity of the humid tropical climate on testicular temperature and seminal quality of Mangalarga Marchador stallions. The thermal profiles of the proximal, middle, and distal zones of the testicles and total surface temperature (TSTT) were recorded using an FLIR E60bx thermal imager. The average air temperature (°C) and relative humidity (%) were obtained 1, 5, 9, 33, and 66 days before semen collection and showed a mean value of 26.5±2.4 and 80.4±6.0 respectively. The scrotal surface temperature was close to 34°C and there was no variation with the age of the stallion, reproductive activity, and characteristics of the ejaculate (P>0.05). The only significant correlations obtained were between TSTT and minor defects (R = 0.41; P<0.05), between TSTT and total defects (R = 0.46; P<0.01), and between TSTT and percentage of morphologically normal sperm (R = -0.46; P<0.05). It was concluded that the Mangalarga Marchador stallions maintained the testicular temperature within favorable conditions for spermatogenesis, demonstrating the efficiency of testicular thermoregulation mechanisms in the Atlantic Forest biome.


O objetivo deste estudo foi determinar o perfil termográfico escrotal e verificar a influência da temperatura e da umidade do clima tropical úmido na temperatura testicular e na qualidade seminal de garanhões Mangalarga Marchador. Os perfis térmicos das zonas proximal, média e distal dos testículos e a temperatura da superfície total (TSTT) foram registrados usando-se um termovisor FLIR E60bx. A temperatura média do ar (° C) e a umidade relativa (%) foram obtidas um, cinco, nove, 33 e 66 dias antes da coleta de sêmen e apresentaram valor médio de 26,5 ± 2,4 e 80,4 ± 6,0, respectivamente. A temperatura da superfície escrotal foi próxima a 34°C, e não houve variação com a idade do garanhão, a atividade reprodutiva e as características do ejaculado (P>0,05). As únicas correlações significativas obtidas foram entre TSTT e defeitos menores (R=0,41; P<0,05), entre TSTT e defeitos totais (R=0,46; P<0,01), e entre TSTT e porcentagem de espermatozoides morfologicamente normais (R=-0,46; P<0,05). Concluiu-se que os garanhões Mangalarga Marchador mantiveram a temperatura testicular dentro de condições favoráveis para a espermatogênese, demonstrando a eficiência dos mecanismos de termorregulação testicular no bioma Mata Atlântica.


Subject(s)
Animals , Male , Spermatogenesis/radiation effects , Temperature , Testis , Body Temperature Regulation , Semen Analysis/veterinary , Horses/physiology , Humidity/adverse effects , Semen , Tropical Climate/adverse effects , Thermography/veterinary
2.
Electron. j. biotechnol ; 52: 85-92, July. 2021. graf, tab
Article in English | LILACS | ID: biblio-1283600

ABSTRACT

BACKGROUND: Nonribosomal peptide synthases (NRPS) can synthesize functionally diverse bioactive peptides by incorporating nonproteinogenic amino acids, offering a rich source of new drug leads. The bacterium Escherichia coli is a well-characterized production host and a promising candidate for the synthesis of nonribosomal peptides, but only limited bioprocess engineering has been reported for such molecules. We therefore developed a medium and optimized process parameters using the design of experiments (DoE) approach. RESULTS: We found that glycerol is not suitable as a carbon source for rhabdopeptide production, at least for the NRPS used for this study. Alternative carbon sources from the tricarboxylic acid cycle achieved much higher yields. DoE was used to optimize the pH and temperature in a stirred-tank reactor, revealing that optimal growth and optimal production required substantially different conditions. CONCLUSIONS: We developed a chemically defined adapted M9 medium matching the performance of complex medium (lysogeny broth) in terms of product concentration. The maximum yield in the reactor under optimized conditions was 126 mg L-1, representing a 31-fold increase compared to the first shaking-flask experiments with M9 medium and glycerol as the carbon source. Conditions that promoted cell growth tended to inhibit NRPS productivity. The challenge was therefore to find a compromise between these factors as the basis for further process development.


Subject(s)
Peptide Synthases/metabolism , Bioreactors/microbiology , Escherichia coli , Temperature , Biotechnology , Carbon/metabolism , Models, Statistical , Electrophoresis, Polyacrylamide Gel , Bioengineering , Hydrogen-Ion Concentration
3.
Electron. j. biotechnol ; 51: 88-94, May. 2021. ilus, tab, graf
Article in English | LILACS | ID: biblio-1343452

ABSTRACT

BACKGROUND: Large amounts of b-alanine are required in fine chemical and pharmaceutical synthesis and other fields. Profitable and green methods are required for the industrial production of b-alanine. RESULTS: Replacing endogenous panD of Escherichia coli with heterologous CgpanD from Corynebacterium glutamicum enabled b-alanine synthesis of 0.67 g/L by strain B0016-082BB. Overexpressing CgpanD on both plasmids and chromosomes to enhance the rate-limiting step improved the b-alanine titer to 4.25 g/L in strain B0016-083BB/pPL451-panD with a slighter metabolic burden. Growth factors were introduced by addition of yeast extract, and 6.65 g/L of b-alanine was synthesized by strain B0016- 083BB/pPL451-panD in the M9-3Y medium. CONCLUSIONS: Enhancement of the rate-limiting steps in the b-alanine biosynthetic pathway, recruitment of the temperature-sensitive inducible pL promoter, and optimization of the fermentation process could efficiently increase b-alanine production in E. coli.


Subject(s)
beta-Alanine/biosynthesis , Temperature , Escherichia coli , Fermentation
4.
Electron. j. biotechnol ; 50: 1-9, Mar. 2021. ilus, tab, graf
Article in English | LILACS | ID: biblio-1292302

ABSTRACT

BACKGROUND: Phospholipase D (PLD) is used as the biocatalyst for phosphatidylserine (PS) production. In general, PLD was expressed in insoluble form in Escherichia coli. High-level soluble expression of PLD with high activity in E. coli is very important for industrial production of PLD. RESULTS: Streptomyces chromofuscus PLD coding gene was codon-optimized, cloned without signal peptide, and expressed in E. coli. The optimal recombinant E. coli pET-28a+PLD/BL21(DE3) was constructed with pET-28a without His-tag. The highest PLD activity reached 104.28 ± 2.67 U/mL in a 250-mL shake flask after systematical optimization. The highest PLD activity elevated to 122.94 ± 1.49 U/mL by feeding lactose and inducing at 20 C after scaling up to a 5.0-L fermenter. Substituting the mixed carbon source with 1.0 % (w/v) of cheap dextrin and adding a feeding medium could still attain a PLD activity of 105. 81 ± 2.72 U/mL in a 5.0-L fermenter. Fish peptone from the waste of fish processing and dextrin from the starch are both very cheap, which were found to benefit the soluble PLD expression. CONCLUSIONS: After combinatorial optimization, the high-level soluble expression of PLD was fulfilled in E. coli. The high PLD activity along with cheap medium obtained at the fermenter level can completely meet the requirements of industrial production of PLD.


Subject(s)
Phospholipases/metabolism , Streptomyces/enzymology , Solubility , Streptomyces/genetics , Temperature , Codon , Combinatorial Chemistry Techniques , Escherichia coli
6.
Rev. argent. salud publica ; 13: 1-4, 5/02/2021.
Article in Spanish | LILACS, BINACIS, ARGMSAL | ID: biblio-1291275

ABSTRACT

La publicación de este artículo es una iniciativa organizada por la Alianza Global por el Clima y la Salud (GCHA, por sus siglas en inglés) a través de la Alianza de Salud del Reino Unido sobre el Cambio Climático (UKHACC, por sus siglas en inglés) y los miembros del Grupo de Trabajo Latinoamericano sobre Cambio Climático y Salud. La convocatoria tiene un doble objetivo: que los líderes mundiales acuerden los cambios necesarios para mantener el aumento de la temperatura global por debajo de 1,5°C y que los profesionales de la salud adquieran una mayor relevancia en el debate global sobre la crisis climática y la pérdida de la biodiversidad. La convocatoria se desarrolla en el contexto de la próxima celebración de la Asamblea General de Naciones Unidas que se iniciará el 14 de septiembre de 2021, así como también de la celebración de la conferencia climática COP26 que este año se realizará en Glasgow. Este artículo, cuya autoría pertenece principalmente a editores de 18 prestigiosas revistas científicas ampliamente representativas de los continentes y disciplinas de la salud del mundo, ya ha sido publicada simultáneamente en más de 200 revistas científicas internacionales (https://www.bmj.com/content/full-list-authors-and-signatories-climate-emergency-editorial-september-2021). En esta ocasión, la Revista Argentina de Salud Pública se suma a esta iniciativa conjunta a través de la publicación del artículo en español, con el fin de promover su difusión en los países de la Región. El editorial expresa también la necesidad de restablecer la diversidad biológica y proteger la salud, y de que los países de altos ingresos hagan mayores recortes en sus emisiones de carbono y transfieran fondos a los países de ingresos bajos y medianos. Además, exhorta a la intervención de los gobiernos en el rediseño de los sistemas de transporte, las ciudades, la producción y distribución de alimentos, los mercados para las inversiones financieras, los sistemas de salud, entre otros aspectos, lo que redundaría en amplios beneficios para la salud.


Subject(s)
Temperature , Environmental Health , Biodiversity , Emergencies , Global Warming , COVID-19
7.
Rev. bras. promoç. saúde (Impr.) ; 34(1): 1-9, 17/02/2021.
Article in Spanish | LILACS | ID: biblio-1344653

ABSTRACT

Objetivo: Este artículo de investigación busca conocer la influencia de la propagación del virus COVID-19 a través de la temperatura y de la humedad en España y Brasil. Métodos: Para el cálculo de la variación mensual del índice de propagación del virus COVID-19 por provincias en España se han utilizado, en primer lugar, las series climáticas de la AEMET de España e INMETRO de Brasil. Se han extraído las medias correspondientes y después se han sometido los datos a un proceso de homogenización, para posteriormente poder calcular el incremento mensual de temperatura y de humedad por provincias y estados. Este proceso metodológico establece una relación directamente proporcional entre el aumento de la temperatura y de la humedad con el índice de propagación del virus COVID-19. Resultados: En España, las condiciones climáticas favorecerán la disminución o aumento del índice reproductivo del virus. En Brasil las condiciones climáticas no favorecerán la disminución del índice reproductivo del virus y, climatológicamente, no existe un periodo óptimo para una desescalada y vuelta a la normalidad. Las variaciones de las condiciones climáticas en Brasil no son significativas, por lo que el clima de Brasil no influye en la disminución de propagación del virus. Conclusión: El clima influye en la propagación del virus. Descriptores: COVID-19; Transmisión de Enfermedad Infecciosa; Clima; Temperatura; Humedad.


Objetivo: Este artigo de pesquisa busca conhecer a influência da propagação do vírus COVID-19 através da temperatura e umidade na Espanha e no Brasil. Métodos: Para calcular a variação mensal do índice de propagação do vírus COVID-19 por províncias da Espanha, primeiramente, utilzaram-se as séries climáticas da AEMET da Espanha e do INMETRO do Brasil. Extraíram-se as médias correspondentes, para posterior submissão dos dados a um processo de homogeneização, com o intuito de calcular o aumento mensal de temperatura e umidade por províncias e estados. Esse processo metodológico estabeleceu uma relação diretamente proporcional entre o aumento da temperatura e da umidade com a taxa de disseminação do vírus COVID-19. Resultados: Na Espanha, as condições climáticas favoreceram a diminuição ou aumento do índice reprodutivo do vírus. No Brasil, entretanto, as condições climáticas não favorecem a diminuição do índice reprodutivo do virus, comprovando que climatologicamente não existe um período ideal para uma desaceleração e retorno à normalidade. As variações nas condições climáticas no Brasil não são significativas, portanto o clima não influencia na diminuição da propagação do vírus neste país. Conclusão: O clima influencia a disseminação do vírus. Descritores: COVID-19; Transmissão de Doença Infecciosa; Clima; Temperatura; Umidade.


Objective: This research article seeks to know the influence of the spread of the COVID-19 virus through temperature and humidity in Spain and Brazil. Methods: In order to calculate the monthly variation in the COVID-19 virus spread index by provinces in Spain, at first, the climatic series of the AEMET of Spain and INMETRO of Brazil were used. The corresponding means have been extracted and then the data have been subjected to a homogenization process, to later be able to calculate the monthly increase in temperature and humidity by provinces and states. This methodological process establishes a directly proportional the climatic conditions favored the decrease or increase of the reproductive index of the virus. In Brazil, however, the climatic conditions do not favor the decrease in the reproductive index of the virus, proving that climatologically there is no optimal period for de-escalation and return to normality. The variations in climatic conditions in Brazil are not significant, so the climate does not influence the decrease in the spread of the virus. Conclusion: Climate influences the spread of the virus. Descriptors: COVID-19; Disease Transmission, Infectious; Climate; Temperature; Humidity. relationship between the increase in temperature and humidity with the spread rate of the COVID-19 virus. Results: In Spain the climatic conditions favored the decrease or increase of the reproductive index of the virus. In Brazil, however, the climatic conditions do not favor the decrease in the reproductive index of the virus, proving that climatologically there is no optimal period for de-escalation and return to normality. The variations in climatic conditions in Brazil are not significant, so the climate does not influence the decrease in the spread of the virus. Conclusion: Climate influences the spread of the virus.


Subject(s)
Temperature , Disease Transmission, Infectious , Basic Reproduction Number , COVID-19 , Humidity
8.
Electron J Biotechnol ; 49: 56-63, Jan. 2021. tab, ilus, graf
Article in English | LILACS | ID: biblio-1291900

ABSTRACT

BACKGROUND: 2R,3R-butanediol dehydrogenase (R-BDH) and other BDHs contribute to metabolism of 3R/3S-Acetoin (3R/3S-AC) and 2,3-butanediol (2,3-BD), which are important bulk chemicals used in different industries. R-BDH is responsible for oxidizing the hydroxyl group at their (R) configuration. Bacillus species is a promising producer of 3R/3S-AC and 2,3-BD. In this study, R-bdh gene encoding R-BDH from Bacillus sp. DL01 was isolated, expressed and identified. RESULTS: R-BDH exerted reducing activities towards Diacetyl (DA) and 3R/3S-AC using NADH, and oxidizing activities towards 2R,3R-BD and Meso-BD using NAD+ , while no activity was detected with 2S,3S-BD. The R-BDH showed its activity at a wide range of temperature (25 C to 65 C) and pH (5.0­8.0). The R-BDH activity was increased significantly by Cd2+ when DA, 3R/3S-AC, and Meso-BD were used as substrates, while Fe2+ enhanced the activity remarkably at 2R,3R-BD oxidation. Kinetic parameters of the R-BDH from Bacillus sp. DL01 showed the lowest Km, the highest Vmax, and the highest Kcat towards the racemic 3R/3S-AC substrate, also displayed low Km towards 2R,3R-BD and Meso-BD when compared with other reported R-BDHs. CONCLUSIONS: The R-BDH from Bacillus sp. DL01 was characterized as a novel R-BDH with high enantioselectivity for R-configuration. It considered NAD+ and Zn2+ dependant enzyme, with a significant affinity towards 3R/3S-AC, 2R,3R-BD, and Meso-BD substrates. Thus, R-BDH is providing an approach to regulate the production of 3R/3S-AC or 2,3-BD from Bacillus sp. DL01.


Subject(s)
Bacillus subtilis/enzymology , Alcohol Oxidoreductases/isolation & purification , Alcohol Oxidoreductases/metabolism , Temperature , Kinetics , Hydrogen-Ion Concentration , Acetoin
9.
Article in Chinese | WPRIM | ID: wpr-879261

ABSTRACT

The cold chain safety of vaccines is a global issue. The electronic vaccine vial monitor (eVVM) label can monitor the temperature of vaccines in real time and provide "early warning" prompts. In order to comprehensively evaluate the monitoring efficiency of eVVM, this study selected 75 eVVM labels and distributed them with a total of 600 vaccine vial monitor (VVM) labels of four different types in different experimental environment (2-8℃, -20℃ and 40℃), and used a temperature recorder as "gold standard". The results showed that the accuracy of the eVVM labels and VVM labels in high temperature environment was as same as that of the temperature recorder (


Subject(s)
Drug Storage , Electronics , Refrigeration , Temperature , Vaccines
10.
Article in Chinese | WPRIM | ID: wpr-879153

ABSTRACT

In this paper, the extraction rate of crude polysaccharides and the yield of polysaccharides from Hippocampus served as test indicators. The comprehensive evaluation indicators were assigned by the R language combined with the entropy weight method. The Box-Behnken design-response surface methodology(BBD-RSM) and the deep neural network(DNN) were employed to screen the optimal parameters for the polysaccharide extraction from Hippocampus. These two modeling methods were compared and verified experimentally for the process optimization. This study provides a reference for the industrialization of effective component extraction from Chinese medicinals and achieves the effective combination of modern technology and traditional Chinese medicine.


Subject(s)
Dietary Carbohydrates , Hippocampus , Neural Networks, Computer , Polysaccharides , Temperature
11.
Article in Chinese | WPRIM | ID: wpr-878917

ABSTRACT

With the dropping process of Xuesaitong Dropping Pills(XDP) as the study object, critical factors affecting the quality indicators of pill pass rate, average weight of drop pills and roundness were screened out, so as to deepen the understanding of the dropping process. The critical process units, critical quality attributes and potential critical process influencing factors of XDP were determined by risk analysis and prior knowledge, and then the critical influencing factors were screened out by Plackett-Burman design. First, according to the risk assessment, the critical technique of XDP preparation process was dropping, and then the critical quality attributes of dropping process were pill pass rate, average weight of drop pills and roundness. Then, according to fishbone diagram and failure mode and effects analysis(FMEA), potential critical influencing factors were determined as flow rate, matrix ratio, solid-liquid ratio, feed-liquid temperature, top temperature of condensate, bottom temperature of condensate and dropping distance. Finally, among these seven potential factors, the critical influencing factors were determined as material liquid ratio, dropping distance, top temperature of condensate, bottom temperature of condensate. This study revealed the potential of Plackett-Burman design in screening and understanding the influence of selected factors on XDP dropping process, which could provide a reference for studying the dropping process.


Subject(s)
Drugs, Chinese Herbal , Saponins , Temperature
12.
Chinese Journal of Biotechnology ; (12): 580-592, 2021.
Article in Chinese | WPRIM | ID: wpr-878583

ABSTRACT

A novel β-glucosidase BglD2 with glucose and ethanol tolerant properties was screened and cloned from the deep-sea bacterium Bacillus sp. D1. The application potential of BglD2 toward polydatin-hydrolyzing was also evaluated. BglD2 exhibited the maximal β-glucosidase activity at 45 °C and pH 6.5. BglD2 maintained approximately 50% of its origin activity after incubation at 30 °C and pH 6.5 for 20 h. BglD2 could hydrolyze a variety of substrates containing β (1→3), β (1→4), and β (1→6) bonds. The activity of β-glucosidase was enhanced to 2.0 fold and 2.3 fold by 100 mmol/L glucose and 150 mmol/L xylose, respectively. BglD2 possessed ethanol-stimulated and -tolerant properties. At 30 °C, the activity of BglD2 enhanced to 1.2 fold in the presence of 10% ethanol and even remained 60% in 25% ethanol. BglD2 could hydrolyze polydatin to produce resveratrol. At 35 °C, BglD2 hydrolyzed 86% polydatin after incubation for 2 h. Thus, BglD2 possessed glucose and ethanol tolerant properties and can be used as the potential candidate of catalyst for the production of resveratrol from polydatin.


Subject(s)
Enzyme Stability , Glucose , Glucosides/pharmacology , Hydrogen-Ion Concentration , Stilbenes/pharmacology , Substrate Specificity , Temperature , Xylose , beta-Glucosidase/genetics
13.
Article in English | WPRIM | ID: wpr-878325

ABSTRACT

Objective@#The relationship between outdoor temperature and blood pressure (BP) has been inconclusive. We analyzed data from a prospective cohort study in northwestern China to investigate the effect of outdoor temperature on BP and effect modification by season.@*Methods@#A total of 32,710 individuals who participated in both the baseline survey and the first follow-up in 2011-2015 were included in the study. A linear mixed-effect model and generalized additive mixed model (GAMM) were applied to estimate the association between outdoor temperature and BP after adjusting for confounding variables.@*Results@#The mean differences in systolic blood pressure (SBP) and diastolic blood pressure (DBP) between summer and winter were 3.5 mmHg and 2.75 mmHg, respectively. After adjusting for individual characteristics, meteorological factors and air pollutants, a significant increase in SBP and DBP was observed for lag 06 day and lag 04 day, a 0.28 mmHg (95% @*Conclusions@#This study demonstrated a significant negative association between outdoor temperature and BP in a high-altitude environment of northwest China. Moreover, BP showed a significant seasonal variation. The association between BP and temperature differed by season and individuals' demographic characteristics (age, gender, BMI), unhealthy behaviors (smoking and alcohol consumption), and chronic disease status (CVDs, hypertension, and diabetes).


Subject(s)
Adult , Blood Pressure/physiology , China/epidemiology , Environmental Exposure/statistics & numerical data , Female , Humans , Male , Middle Aged , Prospective Studies , Risk Factors , Seasons , Temperature
14.
Article in Chinese | WPRIM | ID: wpr-877679

ABSTRACT

On the base of the principle of penetrating moxibustion and in combination with free adjustment devices such as movable U-shaped moxa stick holder and movable clamp, a new type of moxibustion box exerted on the head is designed, with precise positioning and sufficient heat intensity.


Subject(s)
Hot Temperature , Moxibustion , Temperature , Workforce
15.
Article in Chinese | WPRIM | ID: wpr-877650

ABSTRACT

Acupoint is the integration of structure and function. In this paper, the structure and function of acupoint are studied based on energetics. It is viewed that acupoint is the aggregation and release place of energy, acupuncture is the process of applying energy to acupoint, and the variations of speed, direction, time and local temperature are the key factors of acupoint function.


Subject(s)
Acupuncture Points , Acupuncture Therapy , Meridians , Temperature
16.
Article in Chinese | WPRIM | ID: wpr-877573

ABSTRACT

In view of the limitations of the existing moxibustion instruments, i.e. possible accidental injury when using moxibustion instruments, the negative effects of products from moxibustion instruments on treatment efficacy and health of medical staff and patients, a moxibustion instrument with multi-jointed manipulator is designed. This moxibustion instrument could accurately control the temperature, maintain a safe moxibustion distance, automatically process the burning ashes of moxa and selectively handle moxa smoke. The experimental results shows that this instrument could maintain the constant temperature of target acupoint, reduce the risk of empyrosis, and reasonably deal with the products of moxibustion. The purification rate of moxa smoke is 44.9%, which not only ensures the therapeutic effect of moxa smoke, but also reduces the negative effects of high-concentration moxa smoke on the health of medical staff and patients.


Subject(s)
Acupuncture Points , Humans , Moxibustion , Smoke/analysis , Temperature
17.
Article in Chinese | WPRIM | ID: wpr-880436

ABSTRACT

Effects of heat treatment conditions (including temperature and time) on the shape memory recovery and corrosion resistance of NiTi self-expanding vascular stents were studied based on working mechanism and clinical use. The


Subject(s)
Alloys , Corrosion , Hot Temperature , Materials Testing , Stents , Surface Properties , Temperature , Titanium
18.
Cienc. tecnol. salud ; 8(1): 67-81, 2021. il 27 c
Article in Spanish | LILACS, LIGCSA, DIGIUSAC | ID: biblio-1352959

ABSTRACT

Este documento presenta un análisis comparativo de los resultados de un modelo de simulación de clima, datos de reanálisis regionales y datos locales de precipitación y temperatura estacional de veintitrés estaciones me-teorológicas de Guatemala, para detectar señales de la habilidad del modelo a reproducir el clima estacional en un periodo de 3 años (1998-2000). La simulación se realizó con un modelo climático regional (MCR), para su reducción de escala dinámica, las condiciones de frontera se obtuvieron de los datos de reanálisis ERA-Interim. El modelo utilizado fue RegCM, versión 4, y se comparó con los datos de precipitación y temperatura de la Base de datos CRU a nivel regional centroamericano y a nivel nacional con tres instituciones que generan datos globales (CRU, TRMM y GPCP) y los datos locales. Los esquemas convectivos utilizados fueron el esquema de Grell sobre tierra y Emanuel sobre el océano, con 50 km de resolución espacial. Los ajustes realizados a las parametrizaciones generaron buen desempeño a nivel regional Centroamericano y a nivel Guatemala a pesar de perder habilidad en algunas regiones y meses. El modelo reproduce adecuadamente el comportamiento de la precipitación estacional en la mayor parte de la temporada lluviosa. Subestima la temperatura a nivel regional, pero a nivel Guatemala muestra buen ajuste. La comparación con los datos locales observados muestra que el modelo se ajusta para el periodo en estudio; pero, es necesario realizar más experimentos con distintas resoluciones espaciales y temporales y evaluar la persistencia del modelo.


This document presents the results of an analysis on the comparison of the results of a climate simulation model, regional reanalysis data and local data on precipitation and seasonal temperature from twenty-three meteoro-logical stations in Guatemala, to detect signs of the ability of the model to reproduce the seasonal climate over a period of 3 years (1998-2000). The simulation was performed with a regional climate model (RCM), for its dynamic scale reduction, the boundary conditions were obtained from the ERA-Interim reanalysis data. The model used was RegCM, version 4, and it was compared with the precipitation and temperature data from the CRU Database at the Central American regional level and at the national level with three institutions that generate global data (CRU, TRMM and GPCP) and local data. The convective schemes used were the scheme of Grell on land and Emanuel on the ocean, with 50 km of spatial resolution. The adjustments made to the settings generated good performance at the Central American regional level and at the Guatemala level, despite losing skill in some regions and months. The model adequately reproduces the behavior of seasonal precipitation in most of the rainy season. It underestimates the temperature at the regional level but at the Guatemala level it shows a good fit. The comparison with the observed local data shows that the model fits for the period under study, but it is necessary to carry out more experiments with different spatial and temporal resolutions and to evaluate the persistence of the model.


Subject(s)
Seasons , Temperature , Simulation Technique , Rain , Climate , Rainy Season
20.
Einstein (Säo Paulo) ; 19: eAO5484, 2021. tab, graf
Article in English | LILACS | ID: biblio-1286302

ABSTRACT

ABSTRACT Objective Humidity and temperature are fundamental for the balance in the life cycle of living beings and, consequently, for maintaining the well-being of the human population and reducing the prevalence of infectious diseases. Thus, in order to mitigate the impact of climate change, especially in the period when humidity is not the ideal, it is necessary to adopt some assistance measures. The present experimental study aims to elucidate what would be the recommended option to improve the quality of life of the human being and to clarify which resources (air humidifier, bucket of water or wet towel) will be effective to improve the humidity of the air in times of drought and low moisture. Methods The experimental study was carried out with INKBIRD hygrometers allowing the analysis of the variation of air humidity throughout the day. Three forms of treatment were established: humidifier, wet towel and bucket of water. In each room, two hygrometers were placed equidistant from the occupant of the room and their respective treatment that varied between 1m and 2m away from the headboard indoor each room. In addition, two environments were used as controls, one being an external environment and the other an internal closed environment, totaling five rooms for the study. The rooms were monitored between the end of July and the end of August 2019 in Goiania (GO). Results Although assistance measures are used to significantly improve air pollution in times of extreme drought, there was a significant difference between them. The humidifier and a wet towel had 7.50% and 5.71% more humidity in the external relation (external control), respectively, more efficient. The volume of water, however, did not show significant difference (p>0.05) and, therefore, there was no variation. Conclusion The humidifier and the towel are treatments considered more efficient, and that there was a significant effect of distance on humidity. Therefore, 1m of distance is more efficient in increasing and/or maintaining air humidity, inducing improvements in the populations' health.


RESUMO Objetivo A umidade e a temperatura são fundamentais para o equilíbrio no ciclo da vida dos seres vivos e, consequentemente, para manter o bem-estar da população humana e diminuir a prevalência de doenças infecciosas. Visando mitigar o impacto das alterações climáticas, principalmente no período em que a umidade não é a ideal, é preciso adotar algumas medidas assistencialistas. O presente estudo visa elucidar qual seria a opção mais indicada para melhorar a qualidade de vida do ser humano e esclarecer qual melhor recurso (umidificador de ar, balde com água ou toalha molhada) é eficaz para melhorar a umidade do ar em épocas de seca e baixa umidade. Métodos Estudo experimental realizado com higrômetros INKBIRD que permitiram a análise da variação da umidade do ar ao longo do dia. Foram estabelecidas três formas de tratamento: umidificador, toalha molhada e balde com água. Em cada quarto, foram colocados dois higrômetros equidistantes do ocupante do quarto e seu respectivo tratamento, que variava entre 1m e 2m de distância da cabeceira da cama dentro de cada cômodo. Além disso, dois ambientes foram utilizados como controle, sendo um externo e outro fechado interno, totalizando cinco cômodos para o estudo. Os cômodos foram monitorados entre o final do mês de julho até final do mês de agosto de 2019 em Goiânia (GO). Resultados Apesar de as medidas assistencialistas serem utilizadas para melhora significativa da umidade do ar em épocas de extrema seca, há uma diferença significativa entre elas. O umidificador e a toalha molhada possuíram 7,50% e 5,71% a mais de umidade em relação à área externa (controle externo), respectivamente, sendo considerados mais eficientes. Já o balde de água não se diferenciou significativamente (p>0,05), não havendo variação. Conclusão O umidificador e a toalha foram os tratamentos considerados mais eficientes, com efeito significativo da distância sobre a umidade. Portanto, 1m de distância é mais eficiente no aumento e/ou na manutenção da umidade do ar, induzindo melhorias na saúde da população.


Subject(s)
Humans , Quality of Life , Water , Temperature , Humidity
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