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1.
Article in Chinese | WPRIM | ID: wpr-773143

ABSTRACT

In this study,mouse models of benign prostatic hyperplasia induced by subcutaneous injection of testosterone propionate was used to investigate the therapeutic effect and mechanism of Urtica hyperborean( UW) extracts on prostate hyperplasia in mice. The effects of UW extracts on prostate index,serum epidermal growth factor( EGF) and dihydrotestosterone( DHT) in model mice were observed,and the EGF and anti-apoptotic factor( Bcl-2) mRNA expression levels were detected as well as pathological changes in prostate tissue. The results showed that the ethyl acetate extraction and alcohol soluble fraction of the UW could significantly reduce the prostate index,reduce the serum DHT and EGF levels( P<0. 01),and significantly decrease the EGF and Bcl-2 mRNA expression( P<0. 01),significantly improved the morphological structure of prostate tissue. The above results confirmed that ethyl acetate extract and alcohol-soluble parts of UW have a good preventive effect on mice prostatic hyperplasia model,and its mechanism may be to reduce androgen levels by regulating polypeptide growth factors and/or inhibiting cell hyperproliferation and promoting apoptosis. This study laid the foundation for the further research on UW.


Subject(s)
Animals , Dihydrotestosterone , Blood , Epidermal Growth Factor , Blood , Male , Medicine, Tibetan Traditional , Mice , Plant Extracts , Pharmacology , Prostatic Hyperplasia , Drug Therapy , Proto-Oncogene Proteins c-bcl-2 , Metabolism , Testosterone Propionate , Urticaceae , Chemistry
2.
Article in Chinese | WPRIM | ID: wpr-776582

ABSTRACT

OBJECTIVE@#To study the effects of alcohol administration on benign prostate hyperplasia(BPH) and the reproductive toxicity during development of benign prostate hyperplasia.@*METHODS@#Seventy adult male Kunming mice were randomly divided into seven groups:control (group CON), negative control (group NC, injected subcutaneously with soybean oil, 25 mg/(kg·d), intragastric administration of distilled water, 7.5 ml/(kg·d)), alcohol for 7 and 21 days (group AL7 and AL21, intragastric administration with wine of 50% alcohol, 7.5 ml/(kg·d)), testosterone propionate for 7 and 21 days (group TP7 and TP21, injected subcutaneously with testosterone propionate, 25 mg/(kg·d)), testosterone propionate+alcohol for 7 days (group TP+AL7, injected subcutaneously with testosterone propionate, 25 mg/(kg·d), and intragastric administration with wine of 50% alcohol, 7.5 ml/(kg·d)),10 mice in each groups. Twenty-four hours after the last administration, mice were sacrificed. The indexes of prostate and testis and the parameters of sperm were determined in mice. The levels of free radicals, antioxidation and histopathological changes in testis and prostate were determined.@*RESULTS@#Compared with the control, TP7d group, AL7 and AL21d groups, the prostate coefficient of TP + AL7d group was increased significantly and the quantity and quality of sperm were decreased significantly (0.05).@*CONCLUSIONS@#The typical BPH state could be induced after 7-day treatment of testosterone propionate and alcohol. The testicular and sperm were damaged which enhanced the oxidative stress in reproductive system. The results indicated that alcohol could significantly promote the prostate hyperplasia induced by testosterone propionate in mice.


Subject(s)
Animals , Male , Mice , Plant Extracts , Prostatic Hyperplasia , Testosterone Propionate
3.
Rev. Salusvita (Online) ; 37(3): 645-652, 2018.
Article in Portuguese | LILACS | ID: biblio-1050746

ABSTRACT

Introdução: a redução dos hormônios sexuais está associada à condição como a depressão e, nesse cenário, busca-se cada vez mais por tratamentos de suplementação com testosterona sintética, que possui muitos efeitos colaterais. O Tribulus terrestris L. (TT) é uma planta que supostamente possui compostos químicos similares ou análogos a testosterona. Objetivo: analisar a histologia dos rins de camundongos suíços machos suplementados com TT e propionato de testosterona (PT) Métodos: o procedimento utilizou 30 camundongos suíços machos divididos em 3 grupos, em que o grupo controle recebeu um veículo aquoso durante o experimento. O grupo testosterona recebeu 20mg/kg do fármaco PT e o grupo Tribulus recebeu 10 mg/kg do extrato das flores da planta TT. Após eutanásia, os rins foram retirados e emblocados em parafina para confecção das lâminas. Resultados e Discussão: em comparação ao grupo controle não foram evidenciadas diferenças histológicas. Conclusão: este estudo corrobora com o de Ghanbari (2016), que apresenta um suposto efeito antioxidante nos rins, não causando dano ao mesmo, e sim redução de disfunções renais, danos tubulares, apoptose e estresse oxidativo devido á flavonoides (substâncias encontradas na composição química da planta).


Introduction: the reduction of sex hormones is associated with condition such as depression and, in this scenario, it is increasingly sought for treatments of synthetic testosterone supplementation, which has many side effects. Tribulus terrestris L. (TT) is a plant that supposedly has chemical compounds similar to or analogous to testosterone. Objective: to analyze the histology of the kidneys of male Swiss mice supplemented with TT and testosterone propionate (PT) Methods: the procedure used 30 male Swiss mice divided into 3 groups, where the control group received an aqueous vehicle during the experiment. The testosterone group received 20 mg / kg of the drug PT and the Tribulus group received 10 mg / kg of the extract of the flowers of the TT plant. After euthanasia the kidneys were removed and placed in paraffin for the preparation of the slides. Results and Discussion: in comparison to the control group, no histological differences were found. Conclusion: this study corroborates that of Ghanbari (2016) which presents a supposed antioxidant effect in the kidneys, not causing damage to it, but reduction of renal dysfunctions, tubular damage, apoptosis and oxidative stress due to flavonoids (substances found in the composition chemistry of the plant).


Subject(s)
Mice , Testosterone Propionate , Histology , Mice
4.
Article in English | WPRIM | ID: wpr-717727

ABSTRACT

BACKGROUND/OBJECTIVES: Benign prostatic hypertrophy (BPH) is a major cause of abnormal overgrowth of the prostate mainly in the elderly. Corni Fructus has been reported to be effective in the prevention and treatment of various diseases because of its strong antioxidant effect, but its efficacy against BPH is not yet known. This study was designed to evaluate the therapeutic efficacy of Corni Fructus water extract (CF) in testosterone-induced BPH rats. MATERIALS/METHODS: To induce BPH, rats were intraperitoneal injected with testosterone propionate (TP). Rats in the treatment group were orally administered with CF with TP injection, and finasteride, which is a selective inhibitor of 5α-reductase type 2, was used as a positive control. RESULTS: Our results showed that the increased prostate weight and histopathological changes in BPH model rats were suppressed by CF treatment. CF, similar to the finasteride-treated group, decreased the levels of testosterone and dihydrotestosterone by TP treatment in the serum, and it also reduced 5α-reductase expression and concentration in prostate tissue and serum, respectively. In addition, CF significantly blocked the expression of the androgen receptor (AR), AR co-activators, and proliferating cell nuclear antigen in BPH rats, and this blocking was associated with a decrease in prostate-specific antigen levels in serum and prostate tissue. CONCLUSIONS: These results suggest that CF may weaken the BPH status through the inactivation of at least 5α-reductase and AR activity and may be useful for the clinical treatment of BPH.


Subject(s)
Aged , Animals , Antioxidants , Cornus , Dihydrotestosterone , Finasteride , Humans , Proliferating Cell Nuclear Antigen , Prostate , Prostate-Specific Antigen , Prostatic Hyperplasia , Rats , Receptors, Androgen , Testosterone , Testosterone Propionate , Water
5.
Laboratory Animal Research ; : 111-117, 2018.
Article in English | WPRIM | ID: wpr-717160

ABSTRACT

Benign prostate hyperplasia (BPH) is a male reproductive disease that has gained increasing importance in recent years. The present study investigated whether Pycnogenol® (PYC), a standardized French maritime pine bark extract, could prevent BPH induced by testosterone propionate (TP) in rats. Male Sprague-Dawley rats were randomly divided into five groups of six rats. One group was used as a normal control rats and the other groups received subcutaneous injections of TP for 4 weeks to induce BPH. In the two treatment groups, PYC (20 or 40 mg/kg) was administered daily for 4 weeks by oral gavage concurrently with the induction of TP. All rats were sacrificed at the scheduled termination time, the prostates were weighed, and histopathologic examinations were conducted. Dihydrotestosterone (DHT) levels in serum and the prostate were measured, and the expression of proliferating cell nuclear antigen (PCNA) and Ki-67 proteins was investigated. BPH-treated animals showed increases in the relative weight of the prostate, higher concentrations of DHT in serum and the prostate, and higher expression of PCNA and Ki-67 in the prostate; in contrast, PYC-treated animals had significant reductions in these factors compared with the BPH animals. These findings indicated that PYC inhibited the development of BPH and that this was closely associated with a reduction in DHT concentration.


Subject(s)
Animals , Dihydrotestosterone , Humans , Hyperplasia , Injections, Subcutaneous , Male , Models, Animal , Proliferating Cell Nuclear Antigen , Prostate , Prostatic Hyperplasia , Rats , Rats, Sprague-Dawley , Testosterone Propionate , Testosterone
6.
National Journal of Andrology ; (12): 11-20, 2017.
Article in Chinese | WPRIM | ID: wpr-812817

ABSTRACT

Objective@#To investigate whether androgens can regulate the expression of eNOS in rat corpus cavernosum through AKT3, PIK3CA, CALM, and CAV1 and influence erectile function.@*METHODS@#Thirty-six 8-week-old male SD rats were randomly divided into groups A (4-week control), B (6-week control), C (4-week castration), D (6-week castration), E (4-week castration + testosterone replacement), and F (6-week castration + testosterone replacement). Both the testis and epididymis were removed from the rats in groups C, D, E and F, and on the second day after surgery, the animals of groups E and F were subcutaneously injected with testosterone propionate at 3 mg per kg of the body weight qd alt while all the others with isodose oil instead. At 4 weeks (for groups A, C and E) and 6 weeks (for groups B, D and F) after treatment, we detected the maximum intracavernous pressure (ICPmax), the mean carotid arterial pressure (MAP) and their ratio (ICPmax/MAP), measured the level of serum testosterone (T), and determined the expressions of eNOS, P-eNOS, AKT3, PIK3CA, CALM and CAV1 in the corpus cavernosum by Western blot and immunohistochemistry.@*RESULTS@#No statistically significant differences were observed in the body weight and MAP among different groups. The serum T level and ICPmax/MAP were remarkably lower in groups C and D than in the other four groups (P<0.01) as well as in groups E and F than in A and B (P<0.05) but exhibited no significant differences either between E and F or between A and B. Immunohistochemistry showed that eNOS and P-eNOS were mainly expressed in the vascular endothelial cell membrane and cavernous vascular lumen, while AKT3, PIK3CA, CALM and CAV1 chiefly in the vascular endothelial cell cytoplasm and membrane, with a few in the smooth muscle cells. Western blot analysis manifested that the expressions of eNOS, P-eNOS, AKT3, PIK3CA, CALM and CAV1 were markedly lower in groups C and D than in A, B, E and F (P<0.01) as well as in D than in C (P<0.05) but those in groups E and F did not showed any significant difference from those in A and B, nor E from F or A from B.@*CONCLUSIONS@#Androgens can improve erectile function by upregulating the expressions of AKT3, PIK3CA, CALM and CAV1 protein molecules and activating eNOS after its phosphorylation, though the exact molecular mechanisms are yet to be further studied.


Subject(s)
Animals , Blood Pressure , Blotting, Western , Caveolin 1 , Metabolism , Class I Phosphatidylinositol 3-Kinases , Metabolism , Erectile Dysfunction , Hormone Replacement Therapy , Male , Monomeric Clathrin Assembly Proteins , Metabolism , Myocytes, Smooth Muscle , Nitric Oxide Synthase Type III , Metabolism , Orchiectomy , Penile Erection , Physiology , Penis , Metabolism , Proto-Oncogene Proteins c-akt , Metabolism , Random Allocation , Rats , Rats, Sprague-Dawley , Testosterone Propionate
7.
National Journal of Andrology ; (12): 353-360, 2017.
Article in Chinese | WPRIM | ID: wpr-812760

ABSTRACT

Objective@#To explore the effects of Kudzu Root plus Cinnamon Granules (KR+C) on prostatic hyperplasia (PH) in mice.@*METHODS@#Sixty 4-week-old Kunming male mice were randomly divided into six groups: blank control, PH model, high-, medium- and low-dose KR+C, and finasteride control. All the mice except those in the blank control group were subcutaneously injected with testosterone propionate (5 mg / [kg·d]) at 7 days after surgical castration. The animals of different groups were treated intragastrically with different doses of KR+C, finasteride, and normal saline respectively for 3 weeks and then sacrificed for weighing of the prostate, calculation of the prostatic index, observation of the morphological changes in the prostate after HE staining, determination of the expressions of FGF2, Ki67 and TGF-β1 by immunohistochemistry, detection of 5α-reductase activity by ELISA, and measurement of the apoptosis index of the prostatic cells by TUNEL.@*RESULTS@#Compared with the model controls, the mice of the other groups showed significantly reduced prostatic volume (P 0.05).@*CONCLUSIONS@#KR+C can reduce the prostatic volume of PH mice by decreasing the activity of 5α- reductase, inhibiting the expressions of FGF2, Ki67 and TGF-β1, and promoting the apoptosis of prostatic cells.


Subject(s)
Animals , Apoptosis , Cholestenone 5 alpha-Reductase , Metabolism , Cinnamomum zeylanicum , Chemistry , Fibroblast Growth Factor 2 , Metabolism , Finasteride , Therapeutic Uses , In Situ Nick-End Labeling , Ki-67 Antigen , Metabolism , Male , Mice , Organ Size , Phytotherapy , Methods , Plant Roots , Chemistry , Prostate , Pathology , Prostatic Hyperplasia , Drug Therapy , Metabolism , Pathology , Pueraria , Chemistry , Random Allocation , Testosterone Propionate , Transforming Growth Factor beta1 , Metabolism , Urological Agents , Therapeutic Uses
8.
Article in English | WPRIM | ID: wpr-51186

ABSTRACT

BACKGROUND/OBJECTIVES: This study was conducted to investigate the effect of a corn silk extract on improving benign prostatic hyperplasia (BPH). MATERIALS/METHODS: The experimental animals, 6-week-old male Wistar rats, were divided into sham-operated control (Sham) and experimental groups. The experimental group, which underwent orchiectomy and received subcutaneous injection of 10 mg/kg of testosterone propionate to induce BPH, was divided into a Testo Only group that received only testosterone, a Testo+Fina group that received testosterone and 5 mg/kg finasteride, a Testo+CSE10 group that received testosterone and 10 mg/kg of corn silk extract, and a Testo+CSE100 group that received testosterone and 100 mg/kg of corn silk extract. Prostate weight and concentrations of dihydrotestosterone (DHT), 5α-reductase 2 (5α-R2), and prostate specific antigen (PSA) in serum or prostate tissue were determined. The mRNA expressions of 5α-R2 and proliferating cell nuclear antigen (PCNA) in prostate tissue were also measured. RESULTS: Compared to the Sham group, prostate weight was significantly higher in the Testo Only group and decreased significantly in the Testo+Fina, Testo+CSE10, and Testo+CSE100 groups (P < 0.05), results that were consistent with those for serum DHT concentrations. The concentrations of 5α-R2 in serum and prostate as well as the mRNA expression of 5α-R2 in prostate were significantly lower in the Testo+Fina, Testo+CSE10, and Testo+CSE100 groups than that in the Testo Only group (P < 0.05). Similarly, the concentrations of PSA in serum and prostate were significantly lower in the Testo+Fina, Testo+CSE10, and Testo+CSE100 groups (P < 0.05) than in the Testo Only group. The mRNA expression of PCNA in prostate dose-independently decreased in the Testo+CSE-treated groups (P < 0.05). CONCLUSIONS: BPH was induced through injection of testosterone, and corn silk extract treatment improved BPH symptoms by inhibiting the mRNA expression of 5α-R2 and decreasing the amount of 5α-R2, DHT, and PSA in serum and prostate tissue.


Subject(s)
Animals , Dihydrotestosterone , Finasteride , Humans , Injections, Subcutaneous , Male , Models, Animal , Orchiectomy , Proliferating Cell Nuclear Antigen , Prostate , Prostate-Specific Antigen , Prostatic Hyperplasia , Rats , Rats, Wistar , RNA, Messenger , Silk , Testosterone , Testosterone Propionate , Zea mays
9.
Int. j. morphol ; 32(3): 756-760, Sept. 2014. ilus
Article in English | LILACS | ID: lil-728262

ABSTRACT

The aim of this research was to investigate the effect of testosterone propionate administration in the liver of rats. The rats were divided in the following groups: Initial control (SC), Aged control (SE) and Anabolic group (SA). Testosterone propionate was administered three times per week during 16 weeks. Using morphoquantitative techniques, we quantified the volume densities of lobular and non-lobular parenchyma, area and number of nuclei of hepatocytes. The data were analyzed statistically using mean and standard deviation, ANOVA one-way and level of significance about p0.05. Our results showed an increase in capillaries, perisinusoidal spaces and biliary ducts in SE group compared to SC. SA group showed a decrease in hepatic cells, non-lobular volume density and hepatocytes nuclei area, but also an increase in capillaries, perisinusoidal spaces, biliary ducts, number of hepatocytes and non-hepatocyte nuclei compared to SC. We conclude that a direct toxicity may have occurred, with consequent loss of the cells.


El objetivo fue investigar el efecto de la administración de propionato de testosterona en el hígado de ratas. Las ratas se dividieron en los siguientes grupos: control inicial (CI), control de Edad (CE) y grupo anabólico (GA). El propionato de testosterona se administró tres veces por semana durante 16 semanas. Utilizando técnicas morfocuantitativas, determinamos las densidades de volumen del parénquima lobular y no lobular, área y número de núcleos de los hepatocitos. Los datos fueron analizados estadísticamente con la media y desviación estándar, la prueba de ANOVA de una vía y un nivel de significación p0,05. Nuestros resultados mostraron un aumento en los capilares, espacios perisinusoidales y conductos biliares en el grupo CE en comparación con CI. El GA mostró una disminución en las células hepáticas, la densidad de volumen no lobular y el área de los núcleos de hepatocitos, como también un aumento en los capilares, espacios perisinusoidales, conductos biliares, número de hepatocitos y núcleos no hepatocíticos en comparación AL CI. Concluimos que una toxicidad directa puede haber ocurrido, con la consiguiente pérdida de las células.


Subject(s)
Animals , Male , Rats , Testosterone Propionate/pharmacology , Liver/drug effects , Aging , Analysis of Variance , Rats, Wistar , Hepatocytes/drug effects , Testosterone Propionate/administration & dosage
10.
Singapore medical journal ; : 315-320, 2013.
Article in English | WPRIM | ID: wpr-359088

ABSTRACT

<p><b>INTRODUCTION</b>The hippocampus is an important region of the brain that regulates cognitive and emotional functions. In this study, we examined the impact of perinatal administration of testosterone propionate (TP) on the number of pyramidal neurons in the CA1 and CA3 regions of the hippocampi of female rats.</p><p><b>METHODS</b>Five groups of rats were used in this study. Three groups of female rats were administered TP in either both the prenatal and the postnatal periods (Group 1), only the prenatal period (Group 2) or only the postnatal period (Group 3). The other two groups of rats included control females (Group 4) and control males (Group 5). The rats were sacrificed on postnatal Day 120 and their brains were analysed for hippocampal pyramidal neuron number using stereological methods.</p><p><b>RESULTS</b>Control male rats (Group 5; p = 0.043) and TP-treated female rats in Groups 1 (p = 0.012) and 2 (p = 0.037), but not Group 3 (p > 0.05), had a significantly higher number of pyramidal neurons than control female rats (Group 4). The rats in Group 1 had the highest number of pyramidal neurons among the female rats.</p><p><b>CONCLUSION</b>Perinatal TP treatment has an augmenting effect on the number of pyramidal neurons in the hippocampi of female rats. We also found gender-based differences in the hippocampi of male and female rats, with a higher number of pyramidal neurons seen in male rats. Continuous TP administration during the prenatal and postnatal periods is more effective than administration only in the prenatal or postnatal period.</p>


Subject(s)
Animals , Body Weight , Female , Hippocampus , Cell Biology , Male , Maternal Exposure , Neurons , Pregnancy , Pregnancy, Animal , Prenatal Exposure Delayed Effects , Pyramidal Cells , Rats , Rats, Wistar , Testosterone Propionate , Pharmacology
11.
Braz. j. med. biol. res ; 43(9): 843-852, Sept. 2010. ilus, tab
Article in English | LILACS | ID: lil-556853

ABSTRACT

To determine if Butea superba Roxb., a traditional Thai male potency herb, has androgenic activity in 60-day-old male Wistar rats, we measured its effects on the pituitary-testicular axis and sex organs. Intact and orchidectomized adult male rats were subdivided into five groups (10 rats/group): distilled water, Butea superba (BS)-10, BS-50, BS-250, and testosterone propionate (TP). They received 0, 10, 50, and 250 mg·kg body weight-1·day-1 BS in distilled water by gavage and 6 mg·kg body weight-1·day-1 TP sc, respectively, during the 30-day treatment period. Blood was collected every 15 days and luteinizing hormone (LH), follicle-stimulating hormone (FSH) and testosterone were measured. Changes of weight and histological appearance of sex organs were determined at the end of the 30-day treatment and 15-day post-treatment periods. TP treatment reduced serum FSH and LH levels and significantly increased the weight of the seminal vesicles and epididymis, in accordance with histopathological changes, in both intact and orchidectomized rats. No changes in serum testosterone, LH, and FSH levels were observed in any of the intact rats treated with BS, but a significant increase in seminal vesicle weight was observed only in the BS-250 group. Although a significant reduction in serum LH was detected in the BS-50 and BS-250 groups of orchidectomized rats, no significant change in weight or histology of sex organs was observed. Thus, we conclude that B. superba needs endogenous testosterone to work synergistically to stimulate the accessory sex organ of intact animals and can potentially exhibit an LH reduction effect in orchidectomized animals.


Subject(s)
Animals , Male , Rats , Butea/chemistry , Follicle Stimulating Hormone/blood , Luteinizing Hormone/blood , Plant Extracts/pharmacology , Testosterone/blood , Luteinizing Hormone/drug effects , Orchiectomy , Organ Size/drug effects , Pituitary Gland/drug effects , Radioimmunoassay , Rats, Wistar , Seminal Vesicles/drug effects , Testis/drug effects , Testosterone Propionate/pharmacology
12.
Article in Korean | WPRIM | ID: wpr-48076

ABSTRACT

PURPOSE: Anthocyanin is known as a water soluble natural pigment and potent antioxidant. We extracted anthocyanin mediating antioxidant reaction from black soybeans, administered the extract to rats induced prostatic hyperplasia, and evaluate the effect of anthocyanin. MATERIALS AND METHODS: Twenty four male rats were divided into 4 experimental groups: the control, BPH-induced, BPHinduced, and oral anthocyanin (40 mg/kg, 80 mg/kg)-administered groups. For exclusion of intrinsic testosterone influence, a bilateral orchiectomy was done on all except the control group. An experimental prostate hyperplasia was induced by the subcutaneous administration of 3 mg/kg testosterone propionate for 4 weeks to all except the control group. Anthocyanin administration was done in the last 4 weeks in the anthocyanin-administered groups. After 8 weeks, the prostates were removed and analyzed for their prostatic weight and histological examination. Then TUNEL staining was done on each group's specimens, and they were analyzed for their apoptotic body counts. RESULTS: The mean prostate weight was found to be 674.17+/-28.24 mg, 1,098.33+/-131.31 mg, 323.00+/-22.41 mg, and 324.00+/-26.80 mg in the control, BPH-induced, and oral anthocyanin-administered (40 mg/kg, 80 mg/kg) groups, respectively. The BPH-induced group showed statistically significant increases in their prostate weights compared with the control group (p<0.05) and the anthocyanin administered groups showed statistically significant decreases compared to the control and BPH-induced groups (p<0.05). Histologically injected testosterone led to prostatic hyperplasia, but anthocyanin-administered groups experienced this change to a lesser extent. Apoptotic body counts in 5x400/HPF were found to be 3.67+/-0.86, 1+/-0.94, 15.67+/-2.36, and 28.33+/-1.71 in each group. The anthocyanin-administered groups showed statistically significant increases in apoptotic body counts compared with the control and BPH induced groups (p<0.05). CONCLUSIONS: In a prostatic hyperplasia-induced rat model, administration of anthocyanin showed the reduction of prostate weight and the increase of apoptosis. We thought that such results were caused by antioxidant reactions of anthocyanin, and administration of the anthocyanin may be effective in benign prostatic hyperplasia, which is the representative geriatric disease of the urological system.


Subject(s)
Animals , Anthocyanins , Apoptosis , Humans , Hyperplasia , In Situ Nick-End Labeling , Male , Negotiating , Orchiectomy , Prostate , Prostatic Hyperplasia , Rats , Soybeans , Testosterone , Testosterone Propionate , Weights and Measures
13.
Article in Chinese | WPRIM | ID: wpr-252079

ABSTRACT

<p><b>OBJECTIVE</b>To study the effects of androgen on the expression of phosphacan and NG2 proteoglycan (NG2) and neurite regeneration in neonatal rats with hypoxic-ischemic brain damage (HIBD) and the potential mechanism underlying the protective effect of androgen against HIBD.</p><p><b>METHODS</b>One hundred and twenty neonatal Sprague-Dawley rats were randomly divided into three groups: sham-operated, HIBD and androgen treatment. HIBD was induced by the ligation of left common carotid artery and hypoxia exposure. The androgen treatment group rats were injected with testosterone propionate (25 mg/kg) immediately after HIBD. Phosphacan and NG2 expression in the cortex and the hippocampus was detected with the immunohistochemical method 24 and 72 hrs and 7 and 10 days after hypoxia-ischemia (HI). The ultrastructure and neurite regeneration of neurons in the cortex and the hippocampus were observed under a transmission electron microscope.</p><p><b>RESULTS</b>The neurite regeneration was obvious in the sham-operated group, but seldom in the HIBD group. The androgen treatment group showed increased neurite regeneration compared with the HIBD group. There were fewer phosphacan and NG2 positive cells in the cortex and the hippocampus in the sham-operated group. Phosphacan and NG2 expression in the cortex and the hippocampus was observed at 24 hrs, increased at 72 hrs, and peaked at 7 days after HI in the HIBD group and remained at a higher expression 10 days after HI than in the sham-operated group. The levels of phosphacan and NG2 expression in the cortex and the hippocampus in the androgen treatment group were significantly reduced compared with those in the HIBD group 24 and 72 hrs and 7 and 10 days after HI (P<0.01).</p><p><b>CONCLUSIONS</b>Phosphacan and NG2 may be important inhibitory factors for neurite regeneration following HIBD in neonatal rats. The neuroprotection of androgen against neonatal HIBD is produced possibly through an inhibition of phosphacan and NG2 expression.</p>


Subject(s)
Animals , Animals, Newborn , Antigens , Brain Chemistry , Female , Hypoxia-Ischemia, Brain , Immunohistochemistry , Male , Microscopy, Electron, Transmission , Nerve Regeneration , Neurites , Physiology , Proteoglycans , Random Allocation , Rats , Rats, Sprague-Dawley , Receptor-Like Protein Tyrosine Phosphatases, Class 5 , Testosterone Propionate , Pharmacology
14.
Article in Chinese | WPRIM | ID: wpr-292958

ABSTRACT

<p><b>OBJECTIVE</b>To explore gene expression of estrogen receptor (ERalpha, ERbeta) and androgen receptor (AR) in genioglossal muscle (GG) of adult male rats, and to investigate the effects of sex hormones on GG activities, ERalpha, ERbeta and AR expression.</p><p><b>METHODS</b>GG samples were collected from 10 healthy adult male rats. Total RNA were extracted and subjected to fluorescent quantitative reverse transcription-polymerase chain reaction (FQ RT-PCR) for quantitative measurement of ERalpha, ERbeta and AR mRNAs. The other 24 male rats were randomly divided into 3 groups: control group, estrogen group (intramuscular injection of estrogen 0.1 mg/kg, twice a week) and androgen group (intramuscular injection of androgen 2.5 mg/kg, twice a week). The electromyographic activities (EMG) and contract tension of GG were investigated after 4-week treatment. The expression of ERalpha, ERbeta and AR was assessed by Western blot.</p><p><b>RESULTS</b>The mRNA expression ratios of AR/GAPDH, ERalpha/GAPDH, ERbeta/GAPDH and ERalpha/ERbeta were (295.80 +/- 127.20), (2042.00 +/- 921.57), (65.96 +/- 29.57) and (36.83 +/- 19.66), respectively. The mRNA level of ERalpha was significantly higher than that of ERbeta (P < 0.01). Compared with the control group, the EMG of GG was intensified in the estrogen group (P < 0.01). GG contractility did not change significantly (P > 0.05), and ERalpha expression in GG was up-regulated by estrogen (P < 0.05); while in the androgen group, the EMG of GG was weakened (P < 0.05). P(t) and P(0) were slightly increased (P > 0.05) and the decline rate of P(0) was markedly quickened (P < 0.05). AR and ERbeta expressions were down-regulated by androgen (P < 0.05).</p><p><b>CONCLUSIONS</b>Both AR and ER were expressed in GG of adult male rats, and ERalpha was expressed more abundantly than ERbeta. Estrogen could greatly improve activities of GG and stimulate the expression of ERalpha. Whereas, androgen could restrain activities of GG, impair its fatigue resistance capacity and inhibit the expression of AR and ERbeta.</p>


Subject(s)
Animals , Estradiol , Pharmacology , Gene Expression Regulation , Male , Pharyngeal Muscles , Metabolism , Physiology , RNA, Messenger , Genetics , Metabolism , Random Allocation , Rats , Rats, Sprague-Dawley , Receptors, Androgen , Genetics , Metabolism , Receptors, Estrogen , Genetics , Metabolism , Reverse Transcriptase Polymerase Chain Reaction , Testosterone Propionate , Pharmacology , Tongue , Metabolism , Physiology
15.
National Journal of Andrology ; (12): 1007-1009, 2006.
Article in Chinese | WPRIM | ID: wpr-289089

ABSTRACT

<p><b>OBJECTIVE</b>To detect the effects of vasectomy on the growth and apoptosis of prostatic tissue cells.</p><p><b>METHODS</b>Forty-five SD rats were divided at random into three groups of fifteen each. Vasectomy (Vsm) model was established by ligating the bilateral vas deferens of the rats in Group A and testosterone propionate and normal saline were subcutaneously injected in those of Groups B and C, respectively. The area percentage of each part in the prostatic tissue was measured with computer-assisted image analysis system. The apoptotic rate was examined with TUNEL.</p><p><b>RESULTS</b>The ratio of stromal area to epithelial plus lumen area in the prostatic tissues in Groups A, B and C were (29.20 +/- 6.85), (39.77 +/- 7.58) and (48.90 +/- 6.49), respectively, and the differences were significant statistically (P all < 0.05). The apoptosis rates in Groups A, B and C were (6.39 +/- 0.84)%, (2.62 +/- 0.57)% and (4.58 +/- 0.93)%, respectively, significantly higher in Group A than in Groups B and C (P all < 0.05).</p><p><b>CONCLUSION</b>Vasectomy may induce interstitium reduction and cell apoptosis in the prostatic tissues of rats, which may help prevent benign prostatic hyperplasia.</p>


Subject(s)
Animals , Apoptosis , Image Processing, Computer-Assisted , In Situ Nick-End Labeling , Male , Prostate , Cell Biology , Random Allocation , Rats , Rats, Sprague-Dawley , Testosterone Propionate , Vasectomy
16.
Article in English | WPRIM | ID: wpr-357792

ABSTRACT

<p><b>OBJECTIVE</b>Some research has shown that androgen has a neuroprotection against hypoxia-ischemia brain damage (HIBD). However, the relevant mechanism has not been fully elucidated. This study aimed to explore the neuroprotection of androgen against HIBD in neonatal rats and the possible mechanism.</p><p><b>METHODS</b>Sixty-four seven-day-old Sprague-Dawley (SD) rats were randomly assigned into three groups: Sham-operation, HIBD and Androgen. The HIBD model was induced by ligation of the left carotid common artery along with hypoxia exposure in neonatal rats from the latter two groups. The Sham-operation group was not subjected to hypoxia-ischemia (HI). The Androgen intervention group received an injection of testosterone propionate (25 mg/kg) immediately after HIBD. Bcl-2 and Bax protein expressions in the cortex and hippocampal CA region were detected by immunohistochemical method at 6, 24 and 72 hrs and at 7 days after HI. The contents of SOD and MDA in the brain tissue homogenate were measured by the thiobarbituric acid (TBA) method and the xanthine oxidase luminescence method respectively at 6, 24 and 48 hrs after HI.</p><p><b>RESULTS</b>There were few Bcl-2 and Bax immune positive cells in the cortex or hippocampus in the left hemisphere in the Sham-operation group at 6 hrs after operation. This was significantly different from the HIBD control and Androgen intervention groups (P < 0.01). The expression of Bcl-2 protein in the cortex and hippocampus of the Androgen intervention group was significantly higher than that of the HIBD control group at 6, 24 and 72 hrs after HI (P < 0.05 or 0.01). The expression of Bax protein in the cortex and hippocampus of the Androgen intervention group was significantly lower than that of the HIBD control group at 24 hrs after HI (P < 0.05). The SOD content in the brain tissue homogenate of the HIBD control group was significantly reduced, in contrast, the MDA content in the brain tissue homogenate of the HIBD control group increased significantly at 6 hrs after HI compared with the Sham-operation group (P < 0.05). The SOD content was reduced to a nadir and the MDA content increased to a peak at 24 hrs after HI in the HIBD control group. Androgen intervention increased significantly the SOD activity at 6,24 and 48 hrs after HI and decreased significantly the MDA content at 6 and 24 hrs after HI as compared with the HIBD control group (P < 0.05 or 0.01).</p><p><b>CONCLUSIONS</b>The neuroprotection of androgen against neonatal HIBD is produced possibly through an increase of Bcl-2 protein expression and a reduction in Bax protein expression, thus decreasing neuronal apoptosis after HI. There may also be a reduction in the consumption of antioxidant and an inhibition of the formation of oxidant free radicals to alleviate neuronal damage following HI.</p>


Subject(s)
Animals , Animals, Newborn , Brain Chemistry , Female , Hypoxia-Ischemia, Brain , Drug Therapy , Male , Malondialdehyde , Neuroprotective Agents , Therapeutic Uses , Proto-Oncogene Proteins c-bcl-2 , Rats , Rats, Sprague-Dawley , Superoxide Dismutase , Metabolism , Testosterone Propionate , Pharmacology , Therapeutic Uses , bcl-2-Associated X Protein
17.
Korean Journal of Urology ; : 1289-1293, 2006.
Article in Korean | WPRIM | ID: wpr-168038

ABSTRACT

PURPOSE: Benign prostatic hyperplasia (BPH) is one of the common diseases in elderly men. Recently, the old-aged population has increased, with the interest in the clinical importance of BPH ever growing. Catechin, an extract of green tea, has the effect of the 5-alpha reductase inhibitor. Typically, BPH has been shown to be influenced by 5-alpha reductase. Therefore, the relationship between BPH and catechin was evaluated. MATERIALS AND METHODS: An experimental prostatic hyperplasia was induced in male Wistar rats by the administration of testosterone propionate, 3mg/kg sc, for 4 weeks. The Wistar rats were divided into four experimental groups: the control, BPH-induced, oral finasteride ingestion and oral catechin ingestion groups. After 4 weeks, the prostates were removed, and analyzed for their prostatic weight and histological examination. RESULTS: The prostate weights were measured in each group, and found to be 330.0+/-40.7, 970.0+/-1.1, 358.0+/-39.9 and 415.0+/-45.3mg in the control, BPH-induced, oral finasteride ingestion and oral catechin ingestion groups, respectively. The oral finasteride and catechin ingestion groups showed statistically significant decreases in their prostatic weights compared with the BPH-induced group (p0.05). Histologically injected testosterone lead to prostatic hyperplasia in rats, but oral catechin ingestion decreased this change. CONCLUSIONS: These results suggest that catechin may be effective in BPH, and the consumption of green tea may be effective in preventing BPH.


Subject(s)
Aged , Animals , Catechin , Cholestenone 5 alpha-Reductase , Eating , Finasteride , Humans , Male , Models, Animal , Oxidoreductases , Prostate , Prostatic Hyperplasia , Rats , Rats, Wistar , Tea , Testosterone , Testosterone Propionate , Weights and Measures
18.
Article in Chinese | WPRIM | ID: wpr-318825

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of testosterone propionate (TP) on the distribution pattern of calcitonin gene-related peptide (CGRP) in two types of motoneuron (Mn) pools in rats.</p><p><b>METHOD</b>The double labeling of cholera toxin B subunit coupled with colloidal gold (CB-Au) retrograde identification combining with immunocytochemistry was mainly used to reveal the distribution pattern of CGRP-like immunoreactivity (CGRP-LI) and its changes in the motoneuron pools labeled by CB-Au.</p><p><b>RESULT</b>TP injected intramuscularly 28 days later significantly decreased CGRP expression in Mn pool innervating extensor digitorum longus (EDL, fast-twitch), comparing with corresponding control and castration group respectively (P < 0.001), while no significant effect on Mn pools innervating soleus (SOL, slow-twitch, P > 0.05) was observed.</p><p><b>CONCLUSION</b>EDL-Mn pool is more sensitive to testosterone propionate than SOL-Mn pool in regulating CGRP expression.</p>


Subject(s)
Animals , Calcitonin Gene-Related Peptide , Metabolism , Male , Motor Neurons , Metabolism , Muscle Fibers, Fast-Twitch , Cell Biology , Muscle Fibers, Slow-Twitch , Cell Biology , Rats , Rats, Wistar , Testosterone Propionate , Pharmacology
19.
Article in Korean | WPRIM | ID: wpr-114512

ABSTRACT

PURPOSE: This study examined the changes in intracavernous pressure, expression of nitric oxide synthase(NOS), and content of penile smooth muscle in castrated rats and testosterone-supplied castrated rats. MATERIALS AND METHODS: Sprague Dawley rats were used for this study and divided into control, castrated, and testosterone-supplied castrated groups. Castration was performed by bilateral orchietomy under general anesthesia, and testosterone propionate 3 mg/kg was injected subcutaneously daily for a week beginning 4 weeks after orchiectomy. Intracavernous pressure was measured by stimulating the cavernous nerve at 10 volts, 2.4 mA. Expression of NOS was measured by immunohistochemical staining for NADPH diaphorase, and content of penile smooth muscle was measured by H&E staining of the corpus cavernosum. The stained area-to-tissue ratio was calculated by computer scanning for each case. RESULTS: Compared with the control group(3.45+/-0.25 ng/ml), the serum testosterone level of the castrated group (0.78+/-0.34 ng/ml) was lower. Serum testosterone level was restored in the testosterone-supplied castrated group. Compared with the(67.2+/-14.3 cmH2O) was decreased (p <0.05). There was no significant difference between the testosterone-supplied group(94.7+/-11.4 cmH2O) and control group, so intracavernosal pressure was restored by testosterone treatment. Immunohistochemical staining for NOS showed that NADPH diaphorase was stained as brown nerve fiber. Compared with the control group(37.5+/-2.8%), the NOS activity of the castrated group(7.5+/-2.1%) was significantly decreased(p <0.05). NOS activity was slightly increased in the testosterone-supplied group(47.5+/-2.4%) compared with the control group, but the difference was not statistically significant. Thus, testosterone treatment restored NOS activity after castration. By H&E staining, the content of penile smooth muscle was 76.5+/-2.8% in the control group, but significantly lower in the castrated group(46.2+/-3.4% p <0.05). Smooth muscle content was slightly decreased in the testosterone-supplied group(63.8+/-4.7%) compared with control group, but the difference was not statistically significant. Thus, smooth muscle content was restored by testosterone treatment after castration. CONCLUSIONS: Decline of factors involved in erectile function can be restored by testosterone replacement after castration.


Subject(s)
Anesthesia, General , Animals , Castration , Male , Muscle, Smooth , NADPH Dehydrogenase , Nerve Fibers , Nitric Oxide , Orchiectomy , Penile Erection , Rats , Rats, Sprague-Dawley , Testosterone Propionate , Testosterone
20.
Acta Physiologica Sinica ; (6): 54-59, 2004.
Article in Chinese | WPRIM | ID: wpr-290889

ABSTRACT

Heme oxygenase (HO) is a rate-limiting enzyme for endogenous carbon monoxide (CO) production. Recently it has been suggested that endogenous CO plays an important role in regulating smooth muscle tone. The development of bladder outlet obstruction in men with benign prostates hyperplasia was shown to be related to the prostates smooth muscle tone, but it was not clear whether endogenous HO/CO system mediates prostates smooth muscle activity. To investigate the influence of sex hormone on the expression of heme oxygenase (HO) gene in rat ventral prostate, we created the model of castrated male rats to test the mRNA levels of HO-1 and HO-2 by RT-PCR, and used immunohistochemical staining procedures with image analytic technical system to confirm the effects of exogenous androgen and estrogen on the expression of HO-1 and HO-2 protein in rat ventral prostate. The results showed that two isoforms of HO were present in rat ventral prostate. The epithelial cells of acini and fibromuscular stroma of the rat prostate displayed HO-1 immunoreactivity, whereas HO-2 immunostaining was only examined to be in the acinar cells. Both at protein and transcript levels, HO-1 in castrated group was markedly decreased compared with the normal control group (p<0.01). In groups of exogenous administration of androgen and estrogen HO-1 was much higher than that in the control groups (p<0.01). However, estrogen increased HO-1 protein level in prostate stroma, while the levels of HO-2 did not give any evidence of change among all groups (p>0.05). These findings suggest that expression of HO-1 gene is induced by sex hormones, in contrast, there is no change in HO-2 expression. We speculate that CO-HO system is possibly involved in the pathologic processes of prostates abnormal proliferation induced by sex hormones and that CO derived from HO-1 may play an important role in the regulation of smooth muscle activity in rat prostate.


Subject(s)
Animals , Carbon Monoxide , Metabolism , Heme Oxygenase (Decyclizing) , Genetics , Heme Oxygenase-1 , Male , Orchiectomy , Prostate , Metabolism , RNA, Messenger , Genetics , Random Allocation , Rats , Rats, Sprague-Dawley , Testosterone Propionate , Pharmacology
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