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1.
Braz. arch. biol. technol ; 63: e20180637, 2020. tab, graf
Article in English | LILACS | ID: biblio-1132189

ABSTRACT

Abstract This study describes the use of bentonite in suspension for the caffeine adsorption (pollutant of emerging concern) by taking different conditions of the pH, adsorbent mass, adsorbent calcination temperature and interferents into account. The results were compared with those obtained using bentonite immobilized in alginate beads. The acid medium has a greater efficiency for the caffeine adsorption and the adsorbent calcination temperature exerts, due to structural changes. Caffeine removal higher than 90% was obtained at optimized conditions. The Langmuir model indicated a better fit of the data and the adsorption capacity of caffeine onto bentonite. The bentonite immobilized led to a slower adsorption process in relation to the suspended.


Subject(s)
Water Pollutants, Chemical/isolation & purification , Bentonite/chemistry , Caffeine/chemistry , Thermodynamics , Caffeine/adverse effects , Adsorption , Environmental Pollutants/isolation & purification , Hot Temperature , Hydrogen-Ion Concentration , Models, Theoretical
2.
Article in English | WPRIM | ID: wpr-827442

ABSTRACT

OBJECTIVE@#To seek potential Chinese herbal medicine (CHM) for the treatment of coronavirus disease 2019 (COVID-19) through the molecular docking of the medicine with SARS-CoV-2 3CL hydrolytic enzyme and the angiotensin converting enzyme II(ACE2) as receptors, using computer virtual screening technique, so as to provide a basis for combination forecasting.@*METHODS@#The molecular docking of CHM with the SARS-Cov-2 3CL hydrolase and the ACE2 converting enzyme, which were taken as the targets, was achieved by the Autodock Vina software. The CHM monomers acting on 3CLpro and ACE2 receptors were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform, the active ingredients were selected, and the key CHMs and compounds were speculated. Based on the perspective of network pharmacology, the chemical-target network was constructed, and the functional enrichment analysis of gene ontology and the pathway enrichment analysis of Kyoto encyclopedia of genes and genomes were carried out by DAVID to speculate about the mechanism of action of the core drug pairs.@*RESULTS@#There are 6 small molecule compounds that have the optimal binding energy with the two target proteins. Among 238 potential anti-COVID-19 herbs screened in total, 16 kinds of CHM containing the most active ingredients, and 5 candidate anti-COVID-19 herbs that had been used in high frequency, as well as a core drug pair, namely, Forsythiae Fructus-Lonicerae Japonicae Flos were selected.@*CONCLUSION@#The core drug pair of Forsythiae Fructus-Lonicerae Japonicae Flos containing multiple components and targets is easy to combine with 3CLpro and ACE2, and exerts an anti-COVID-19 pneumonia effect through multi-component and multi-target, and plays the role of anti-COVID-19 pneumonia in multi-pathway.


Subject(s)
Betacoronavirus , Metabolism , Computer Simulation , Coronavirus Infections , Drug Therapy , Drugs, Chinese Herbal , Therapeutic Uses , Gene Ontology , Humans , Molecular Docking Simulation , Pandemics , Peptidyl-Dipeptidase A , Metabolism , Pneumonia, Viral , Drug Therapy , Thermodynamics
3.
Rev. MVZ Córdoba ; 24(2): 7231-7238, mayo-ago. 2019. tab, graf
Article in Spanish | LILACS | ID: biblio-1115244

ABSTRACT

RESUMEN Objetivo. Establecer mediante experimentos por lote la capacidad de remoción, la cinética y termodinámica de adsorción del carbón activado preparado a partir de la cáscara de yuca (Manihot esculenta) en la remoción del colorante azul directo 86. Materiales y métodos. La metodología experimental consistió inicialmente en la preparación del carbón activado por activación química de la cáscara de yuca con H3PO4 y su posterior calcinación a 530°C. En la caracterización se determinaron las propiedades de textura mediante el índice de yodo e índice de azul de metileno, se cuantificaron los grupos funcionales orgánicos ácidos y básicos con el método Boehm, y se realizó el análisis próximo siguiendo las normas ASTM D-2867-70, ASTM D2866 y ASTM D2866-94. En el estudio por lote, el efecto de varios parámetros sobre la capacidad de adsorción fueron evaluados: el pH (2, 4, 8 y 10), la temperatura (25, 30 y 40°C) y la concentración inicial de colorante (20, 40, 60, 80 y 100 mg/L). Tanto las características fisicoquímicas como los ensayos de adsorción del carbón activado preparado a partir de la cáscara de yuca (CAY) fueron comparadas con otro de marca comercial (CAM). Resultados. Los resultados de la caracterización indican que ambos carbones tienen una química de superfície heterogénea, de naturaleza ácida para el CAY y básica para el CAM. La máxima capacidad obtenida fue 6.1 mg/g para el CAY y de 3.7 mg/g para el CAM. Los cálculos termodinámicos indican que la remoción es espontánea y para ambos carbones la cinética se ajusta al modelo de pseudo segundo orden. Conclusiones. El carbón activado obtenido a partir de la cáscara de yuca puede considerarse un adsorbente eficiente en la remoción de colorantes.


ABSTRACT Objective. To establish by means of experimenting by batch the capacity of removal, the kinetics and adsorption thermodynamics of activated carbon prepared from manioc husk (Manihot esculenta) in the removal of direct blue 86 dye. Materials and methods. Firstly, the experimental methodology worked on the preparation of activated carbon by chemical activation of manioc husk with H3PO4 calcined at 530°C. In the characterization the texture properties were determined by means of the blue methylene and iodine indices, the basic and acidic functional groups were quantified by the Boehm method, and the proximate analyses were done following the norms ASTM D2867-70, ASTM D2866 and ASTM D2866-94. During the batch studies, the effect of several parameters over the adsorption capacity was evaluated: pH (2, 4, 8 and 10), temperature (25, 30 and 40°C) and initial concentration of the dye (20, 40, 60, 80 and 100 mg/L). Both physicochemical and adsorption characteristics of the activated carbon from manioc husk (CAY) were compared against those of a commercial brand (CAM). Results. The results of characterization showed that both carbons have a chemistry heterogeneous surface, acidic for CAY and basic for CAM. The maximum capacity obtained was 6.1 mg/g for CAY and 3.7 mg/g for CAM. The thermodynamic calculations showed that the removal was spontaneous. The kinetics for both carbon samples fits a pseudo second-order model. Conclusions. The activated carbon obtained from the manioc husk can be considered an efficient adsorbent for the removal of dyes.


Subject(s)
Thermodynamics , Kinetics , Manihot , Charcoal
4.
Psicol. Estud. (Online) ; 24: e45522, 2019.
Article in Portuguese | LILACS, INDEXPSI | ID: biblio-1012797

ABSTRACT

RESUMO O presente artigo tem como objetivo abordar o conceito de máquina e a consequente apropriação do mesmo nas discussões sobre a subjetividade. É um estudo teórico na interface entre psicologia, filosofia, física e biologia. Nesse sentido, ele se inicia apresentando que, na modernidade, a analogia à máquina foi estendida à compreensão do universo como sendo um relógio preciso e geometricamente previsível em seu funcionar. E se, até o século XVIII, a vida, o corpo e o cosmos foram significados, pela ciência emergente, como uma máquina mecânica (de movimento calculável em sua previsibilidade), no século XIX estes passaram igualmente a serem compreendidos como uma máquina térmica, com seus desdobramentos na física da termodinâmica e na cibernética. No final do século XX, a partir dos trabalhos dos biólogos Francisco Varela e Humberto Maturana, o conceito de máquina autopoiética ganhou relevância nos estudos sobre a vida e sobre a cognição, sendo este apropriado por Félix Guattari no desenvolvimento de seu conceito de produção de subjetividade e suas problematizações em torno dos processos de subjetivação. Assim, o conceito de máquina saiu das limitações de uma leitura mecânica e termodinâmica da realidade, para uma abordagem existencial, processual e inventiva da subjetividade.


RESUMEN El presente artículo tiene como objetivo abordar el concepto de máquina y la consiguiente apropiación del mismo en las discusiones sobre la subjetividad. Es un estudio teórico en la interfaz entre Psicología, Filosofía, Física y Biología. En ese sentido, se inicia presentando que, en la modernidad, la analogía a la máquina se extendió a la comprensión del universo como un reloj preciso y geométricamente previsible en su funcionamiento. Y si, hasta el siglo XVIII, la vida, el cuerpo y el cosmos fueron significados, por la ciencia emergente, como una máquina mecánica (de movimiento calculable en su previsibilidad), en el siglo XIX estos pasaron igualmente a ser comprendidos como una máquina térmica, con sus desdoblamientos en la física de la termodinámica y en la cibernética. A finales del siglo XX, a partir de losestudios de los biólogos Francisco Varela y Humberto Maturana, el concepto de máquina autopoiética ganó relevancia en los estudios sobre la vida y sobre la cognición, siendo éste apropiado por Félix Guattari en el desarrollo de su concepto de producción de subjetividad y sus problemasen torno a los procesos de subjetivación. Así, el concepto de máquina salió de las limitaciones de una lectura mecánica y termodinámica de la realidad, hacia un enfoque existencial, procesal e inventivo de la subjetividad.


ABSTRACT This article aims to address the concept of machine and its consequent appropriation in the discussions on subjectivity. It is a theoretical study at the interface among Psychology, Philosophy, Physics and Biology. In this sense, it starts showing that in the modernity, the analogy to the machine was extended to the understanding of the universe as a precise and geometrically predictable functioning clock. And if, until the eighteenth century, life, body and cosmos were signified by the emerging science as a mechanical machine (of calculable motion in its predictability), in the nineteenth century they also came to be understood as a thermal machine, with its developments in the thermodynamics physics and cybernetics. In the late twentieth century, the concept of autopoietic machine gained relevance in the studies of life and cognition, based on the works of the biologists Francisco Varela and Humberto Maturana, which is appropriated by Félix Guattari for the development of his concept of the production of subjectivity and its problematizations around the subjectivation processes. Thus, the concept of machine has gone beyond the limitations of a mechanical and thermodynamics reading of reality, to an existential, procedural and inventive approach of the subjectivity.


Subject(s)
History, 18th Century , History, 19th Century , History, 20th Century , History, 21st Century , Artificial Intelligence , Cybernetics , Virtual Reality , Physics , Thermodynamics , Mechanics , Machine Learning , Motion
5.
Braz. arch. biol. technol ; 62(spe): e19190021, 2019. tab, graf
Article in English | LILACS | ID: biblio-1055443

ABSTRACT

Abstract Data Centers are growing steadily worldwide, and they are expected to continue growing up to 53% in 2020. Energy efficiency, in high power consumption, is a key venue. There are methodologies to measure this efficiency, one example is using the PUE (Power Usage Effectiveness) index. In this paper is proposed a new index for measuring efficiency at the design stage, the EUED (Energy Usage Effectiveness Design). This index allows to evaluate systems using "free cooling" and adiabatic system. A comparison is performed considering the equipment in the worst situation. The thermodynamics parameter enthalpy is used to calculate the results. This new methodology allows to determine differences between the cities of São Paulo and Curitiba (1.21%) and between Rio de Janeiro and Curitiba (10.61%). The values for the EUED index were 1.245 kW/kW for Curitiba, 1.260 kW/kW for São Paulo and 1.377 kW/kW for Rio de Janeiro, respectively, reaching a difference of 16.86% for Curitiba, 16.19% for São Paulo and 10.31% for Rio de Janeiro in relation to PUE COA (Power Usage Effectiveness Constant Outdoor Air). The advantage of the EUED is that it works with the 8,760 hours in the design phase, using psychometric elements to determine when to use free cooling and evaporative system, and more importantly varying the COP according to environmental characteristics.


Subject(s)
Efficiency , Energy Consumption , Hot Temperature , Thermodynamics
6.
Braz. arch. biol. technol ; 62: e19180470, 2019. tab, graf
Article in English | LILACS | ID: biblio-989423

ABSTRACT

Abstract The effects of gamma radiation (0, 1, 2 and 3 kGy) were used to evaluate the stability and thermal degradation kinetics of anthocyanins, as well as the stability of total phenolic compounds (TPC) and antioxidant activity at different temperatures (4, 25, 35 and 45 °C) during the storage (0, 30, 60, 90 and 120 days) of black rice flour. This flour can be used as ingredient for gluten-free cereal products with higher nutritional value. For this it is necessary to preserve the anthocyanin content during thermal processing and shelf-life periods. At time 0, the dose of 3 kGy provided all of the most available bioactive compounds, raising their antioxidant potential, except for TPC. During the storage at different temperatures up to 120 days, gradual losses occurred in all the analysed parameters. Regarding the total anthocyanin content and TPC, the sample irradiated with a 1 kGy dose remained most stable. The analysis of kinetic data indicated a first-order reaction for the degradation of anthocyanins. The combination of irradiation with different temperatures may improve the shelf-life of black rice flour.


Subject(s)
Radiation , Oryza , Thermodynamics , Phytochemicals , Anthocyanins/chemistry
7.
Article in Chinese | WPRIM | ID: wpr-773226

ABSTRACT

Small molecules with physiological or pharmacological activities need to interact with biological macromolecules in order to function in the body. As the protein with the highest proportion of plasma protein,serum albumin is the main protein binding to various endogenous or exogenous small molecules. Serum albumin interacts with small molecules in a reversible non-covalent manner and transports small molecules to target sites. Bovine serum albumin( BSA) is an ideal target protein for drug research because of its low cost and high homology with human serum albumin. The research on the interaction between drugs and BSA has become a hotspot in the fields of pharmacy,medicine,biology and chemistry. In this research,molecular docking method was used to study the interaction between three small ginsenosides with high pharmacological value( Rg_1,Rb_1,Ro) and bovine serum albumin( BSA),and the binding mode information of three ginsenosides interacting with BSA was obtained. The results of molecular docking showed that ginsenosides and amino acid residues in the active pocket of proteins could be combined by hydrophobic action,hydrogen bonding and electrostatic action. The interaction between small ginsenosides and bovine serum albumin is not the only form,and their interaction has many forms of force. The interaction between these molecules and various weak forces is the key factor for the stability of the complex. The results of this study can provide the structural information of computer simulation for the determination of the interaction patterns between active components and proteins of ginseng.


Subject(s)
Animals , Binding Sites , Cattle , Computer Simulation , Ginsenosides , Chemistry , Molecular Docking Simulation , Protein Binding , Serum Albumin, Bovine , Chemistry , Spectrometry, Fluorescence , Thermodynamics
8.
Chinese Journal of Biotechnology ; (12): 1914-1924, 2019.
Article in Chinese | WPRIM | ID: wpr-771743

ABSTRACT

Genome-scale metabolic network models have been successfully applied to guide metabolic engineering. However, the conventional flux balance analysis only considers stoichiometry and reaction direction constraints, and the simulation results cannot accurately describe certain phenomena such as overflow metabolism and diauxie growth on two substrates. Recently, researchers proposed new constraint-based methods to simulate the cellular behavior under different conditions more precisely by introducing new constraints such as limited enzyme content and thermodynamics feasibility. Here we review several enzyme-constrained models, giving a comprehensive introduction on the biological basis and mathematical representation for the enzyme constraint, the optimization function, the impact on the calculated flux distribution and their application in identification of metabolic engineering targets. The main problems in these existing methods and the perspectives on this emerging research field are also discussed. By introducing new constraints, metabolic network models can simulate and predict cellular behavior under various environmental and genetic perturbations more accurately, and thus can provide more reliable guidance to strain engineering.


Subject(s)
Enzymes , Metabolism , Genome , Genetics , Metabolic Engineering , Metabolic Networks and Pathways , Genetics , Models, Biological , Thermodynamics
9.
Biosci. j. (Online) ; 33(4): 905-913, july/aug. 2017. tab, graf
Article in English | LILACS | ID: biblio-966252

ABSTRACT

Dehydrated garlic is an importance spice in pre-cooked and processed food products, but studies of the drying variables of this product are scarce. Thus, this work aimed to model and study the kinetics of garlic drying at three different temperatures (40, 50 and 60 °C) and analyze the thermodynamic parameters during the process. The drying was analyzed using pre-established mathematical models for agricultural products and equations that describe the thermodynamics of product dehydration. The best mathematical adjustment for all drying temperatures was the Midilli equation. The moisture ratio was seen to decrease during the process for all situations. The effective diffusivity increased with increasing temperature. The enthalpy change was positive during the process and decreased with increasing temperature. The study entropy was negative, indicating a non-spontaneous reaction. In addition, the Gibbs free energy increased with increasing drying temperature. The thermodynamic standards were satisfactory and correctly described the moisture loss by the garlic samples.


O alho desidratado é um tempero de suma importância em produtos pré-cozidos e processados, no entanto, o estudo das variáveis de secagem desse produto é escasso. Diante disso, objetivou-se com esse trabalho estudar a cinética e a modelagem da secagem de alho, em três temperaturas distintas (40, 50 e 60 °C), e analisar os parâmetros termodinâmicos durante o processo. O estudo da secagem foi feito por meio de modelos matemáticos pré-estabelecidos para produtos agrícolas e por equações que descrevem a termodinâmica da desidratação de produtos. O melhor ajuste matemático foi à equação de Midilli para todas as temperaturas de secagem. A razão de umidade apresentou-se decrescente durante o processo para todas as situações. A difusividade efetiva aumentou com o aumento da temperatura. A variação de entalpia apresentou-se positiva durante o processo de secagem e diminui com o aumento da temperatura. A entropia estudada foi negativa, indicando uma reação não-espontânea. E a energia livre de Gibbs aumentou com a elevação da temperatura de secagem. Os padrões termodinâmicos foram satisfatórios e descreveram como ocorreu a perda de umidade pelas amostras de alho.


Subject(s)
Thermodynamics , Kinetics , Food Preservation , Garlic
11.
Protein & Cell ; (12): 590-600, 2017.
Article in English | WPRIM | ID: wpr-756983

ABSTRACT

Entero virus 71 (EV71) causes hand, foot, and mouth disease (HFMD) and occasionally leads to severe neurological complications and even death. Scavenger receptor class B member 2 (SCARB2) is a functional receptor for EV71, that mediates viral attachment, internalization, and uncoating. However, the exact binding site of EV71 on SCARB2 is unknown. In this study, we generated a monoclonal antibody (mAb) that binds to human but not mouse SCARB2. It is named JL2, and it can effectively inhibit EV71 infection of target cells. Using a set of chimeras of human and mouse SCARB2, we identified that the region containing residues 77-113 of human SCARB2 contributes significantly to JL2 binding. The structure of the SCARB2-JL2 complex revealed that JL2 binds to the apical region of SCARB2 involving α-helices 2, 5, and 14. Our results provide new insights into the potential binding sites for EV71 on SCARB2 and the molecular mechanism of EV71 entry.


Subject(s)
Amino Acid Sequence , Animals , Antibodies, Monoclonal , Chemistry , Genetics , Metabolism , Binding Sites , Cell Line , Crystallography, X-Ray , Enterovirus A, Human , Genetics , Allergy and Immunology , Fibroblasts , Virology , Gene Expression , HEK293 Cells , Humans , Immunoglobulin Fab Fragments , Chemistry , Genetics , Metabolism , Lysosome-Associated Membrane Glycoproteins , Chemistry , Genetics , Allergy and Immunology , Mice , Models, Molecular , Protein Binding , Protein Conformation, alpha-Helical , Protein Conformation, beta-Strand , Protein Interaction Domains and Motifs , Receptors, Scavenger , Chemistry , Genetics , Allergy and Immunology , Receptors, Virus , Chemistry , Genetics , Allergy and Immunology , Recombinant Fusion Proteins , Chemistry , Genetics , Allergy and Immunology , Sequence Alignment , Sequence Homology, Amino Acid , Sf9 Cells , Spodoptera , Thermodynamics
12.
Braz. j. pharm. sci ; 52(3): 443-446, July-Sept. 2016. tab, graf
Article in English | LILACS | ID: biblio-828257

ABSTRACT

ABSTRACT The interaction between 6-shogaol, a pharmacologically active ginger constituent, and human serum albumin (HSA), the main in vivo drug transporter, was investigated using isothermal titration calorimetry (ITC). The value of the binding constant, Ka (5.02 ± 1.37 × 104 M−1) obtained for the 6-shogaol-HSA system suggested intermediate affinity. Analysis of the ITC data revealed feasibility of the binding reaction due to favorable enthalpy and entropy changes. The values of the thermodynamic parameters suggested involvement of van der Waals forces, hydrogen bonds and hydrophobic interactions in the 6-shogaol-HSA complex formation.


Subject(s)
Ginger/anatomy & histology , Thermodynamics , Biological Products/pharmacokinetics , Calorimetry/statistics & numerical data , Serum Albumin/analysis
13.
Pakistan Journal of Pharmaceutical Sciences. 2016; 29 (2): 521-527
in English | IMEMR | ID: emr-176385

ABSTRACT

Micellization is the most important property of surface agents. It plays an important role in the manufacture of pharmaceutical products. The surfactants have many applications in industry, agriculture, mining and oil recovery with functional properties as wetting, foaming and emulsifier in pharmaceutical and cosmetic products. The micellization parameters of surfactants help the manufacture of pharmaceutical products to be appropriate and stable. Therefore, in this study, Polyoxyethylene lauryl ether [C[12]E[23]], Polyoxyethylene [10] cetyl ether [C[16]E[10]] and Polyoxyethylene [20] cetyl ether [C[16]E[20]] were chosen as the nonionic surfactants to examine the effect of temperature variation [10-80[degree]C] on the Critical Micelle Concentration [CMC]. The measurement of surface tension was done by a Du Nöuys ring method. The value of CMC was obtained from the surface tension vs. surfactant concentration curve. Since the temperature was increased, the CMC initially decreased and then increased for each surfactant because the formation of the hydrogen bond is harder in the high temperatures. The surface tension gamma CMC for all three surfactant solutions decreased monotonically as the temperature increased. delta G[degree]m, delta H[degree]m and delta S[degree]m as the thermodynamic parameters of micellization, were also estimated and analyzed. The delta G[degree]m was decreased [10-80[degree]C] if the temperature was increased. The entropy and enthalpy correlation of micellization showed a significant linearity. For C[12]E[23], C[16]E[20] and C[16]E[10], the compensation temperature [Tc] was obtained 309.5, 313.2 and 314.4 K, respectively. The calculated thermodynamic parameters showed that the entropy influenced on the micellization process at lower temperature, but it affected by enthalpy when temperature was increased


Subject(s)
Polyethylene Glycols , Thermodynamics , Temperature , Micelles , Surface Tension
14.
Article in Korean | WPRIM | ID: wpr-58328

ABSTRACT

PURPOSE: To evaluate the effect and prognostic factors of automated thermodynamic treatment (thermal compression therapy device [KCL 1100®]) for Meibomian gland dysfunction (MGD). METHODS: Patients (48 eyes of 24 subjects) with MGD were recruited for a prospective clinical trial. Patients received 15-minute treatments twice a day using the KCL 1100®. Severity of dry eye symptoms were evaluated using the Standard Patient Evaluation for Eye Dryness (SPEED) and Ocular Surface Disease Index (OSDI), and severity of Meibomian gland function was evaluated using the Meibomian gland expressibility (MGE), Meibomian gland secretion (MGS) score and lipid layer thickness measured by LipiView®. To evaluate ocular surface, we measured tear break-up time (BUT) and fluorescein corneal staining score (Oxford scale). Data were presented for baseline and at 2 weeks and 1 month post-treatment. RESULTS: Dry eye symptom (SPEED, OSDI), Meibomian gland function (MGE, MGS), and ocular surface index (BUT, Oxford scale) of patients were significantly improved from baseline to 2 weeks (p 0.05). There were no significant adverse events during the treatment. CONCLUSIONS: KCL 1100® automated thermodynamic treatment is an effective and safe treatment for MGD. Additionally, KCL 1100® is more effective in patients with moderate dry eye symptom and MGD.


Subject(s)
Fluorescein , Humans , Meibomian Glands , Prospective Studies , Tears , Thermodynamics
15.
Protein & Cell ; (12): 17-27, 2016.
Article in English | WPRIM | ID: wpr-757179

ABSTRACT

ABC transporters form the largest of all transporter families, and their structural study has made tremendous progress over recent years. However, despite such advances, the precise mechanisms that determine the energy-coupling between ATP hydrolysis and the conformational changes following substrate binding remain to be elucidated. Here, we present our thermodynamic analysis for both ABC importers and exporters, and introduce the two new concepts of differential-binding energy and elastic conformational energy into the discussion. We hope that the structural analysis of ABC transporters will henceforth take thermodynamic aspects of transport mechanisms into account as well.


Subject(s)
ATP-Binding Cassette Transporters , Physiology , Adenosine Triphosphate , Metabolism , Animals , Humans , Models, Theoretical , Thermodynamics
16.
Rev. chil. pediatr ; 86(4): 283-286, ago. 2015.
Article in English | LILACS | ID: lil-764086

ABSTRACT

Chromosome 22q11.2 deletion syndrome, or DiGeorge syndrome, or velocardiofacial syndrome, is one of the most common multiple anomaly syndromes in humans. This syndrome is commonly caused by a microdelection from chromosome 22 at band q11.2. Although this genetic disorder may reflect several clinical abnormalities and different degrees of organ commitment, the clinical features that have driven the greatest amount of attention are behavioral and developmental features, because individuals with 22q11.2 deletion syndrome have a 30-fold risk of developing schizophrenia. There are differing opinions about the cognitive development, and commonly a cognitive decline rather than an early onset intellectual disability has been observed. We report a case of 22q11.2 deletion syndrome with both early assessment of mild intellectual disabilities and tetralogy of Fallot as the only physic manifestation.


El síndrome del cromosoma 22q11.2, también conocido como supresión o síndrome de DiGeorge o síndrome velocardiofacial, es uno de los síndromes más comunes de anomalías múltiples en los seres humanos. Este síndrome es comúnmente causado por una microdeleción del cromosoma 22 en q11.2 banda. Aunque este trastorno genético muestra varias anomalías clínicas y diferentes grados de compromiso orgánico, las características clínicas que han atraído la mayor atención son el comportamiento y el desarrollo, porque las personas con síndrome de deleción 22q11.2 tienen un riesgo 30 veces mayor de desarrollar esquizofrenia. Hay diferentes opiniones sobre el desarrollo cognitivo, y comúnmente se se ha observado un deterioro cognitivo en lugar de un inicio temprano de discapacidad intelectual. Presentamos un caso de síndrome de deleción 22q11.2 tanto con la evaluación temprana de discapacidades intelectuales leves como con la tetralogía de Fallot como única manifestación física.


Subject(s)
Fibrinogen/chemistry , Nanostructures/chemistry , Crystallization/methods , Freeze Drying/methods , Humidity , Protein Conformation , Scattering, Small Angle , Solubility , Thermodynamics , Water/chemistry , X-Ray Diffraction/methods , X-Rays
17.
Clinics ; 70(5): 363-368, 05/2015. tab, graf
Article in English | LILACS | ID: lil-748276

ABSTRACT

OBJECTIVES: To evaluate the clinical outcomes and identify the predictors of mortality in elderly patients undergoing peritoneal dialysis. METHODS: We conducted a retrospective study including all incident peritoneal dialysis cases in patients ≥65 years of age treated from 2001 to 2014. Demographic and clinical data on the initiation of peritoneal dialysis and the clinical events during the study period were collected. Infectious complications were recorded. Overall and technique survival rates were analyzed. RESULTS: Fifty-eight patients who began peritoneal dialysis during the study period were considered for analysis, and 50 of these patients were included in the final analysis. Peritoneal dialysis exchanges were performed by another person for 65% of the patients, whereas 79.9% of patients preferred to perform the peritoneal dialysis themselves. Peritonitis and catheter exit site/tunnel infection incidences were 20.4±16.3 and 24.6±17.4 patient-months, respectively. During the follow-up period, 40 patients were withdrawn from peritoneal dialysis. Causes of death included peritonitis and/or sepsis (50%) and cardiovascular events (30%). The mean patient survival time was 38.9±4.3 months, and the survival rates were 78.8%, 66.8%, 50.9% and 19.5% at 1, 2, 3 and 4 years after peritoneal dialysis initiation, respectively. Advanced age, the presence of additional diseases, increased episodes of peritonitis, the use of continuous ambulatory peritoneal dialysis, and low albumin levels and daily urine volumes (<100 ml) at the initiation of peritoneal dialysis were predictors of mortality. The mean technique survival duration was 61.7±5.2 months. The technique survival rates were 97.9%, 90.6%, 81.5% and 71% at 1, 2, 3 and 4 years, respectively. None of the factors analyzed were predictors of technique survival. CONCLUSIONS: Mortality was higher in elderly patients. Factors affecting mortality in elderly patients included advanced age, ...


Subject(s)
Gene Expression Regulation , Bacterial Proteins/chemistry , Computational Biology , Kinetics , Ligands , Nucleic Acid Conformation , Nucleotides/chemistry , Protein Binding , Protein Conformation , Protein Structure, Secondary , Protein Structure, Tertiary , RNA , Thermodynamics
18.
Ciênc. saúde coletiva ; 20(1): 139-144, 01/2015.
Article in English | LILACS | ID: lil-733133

ABSTRACT

This paper analyses some aspects of the trajectory of the Argentinian physician and sociologist Juan César García (1932-1984) in the field of Latin American Social Medicine. Three dimensions constituting his basic orientations are highlighted: the elaboration of systematic and reflective social thought; a critical attitude in questioning teaching and professional practices; a commitment to the institutionalization and dissemination of health knowledge.


O artigo analisa aspectos da trajetória de Juan César García (1932-1984), médico e sociólogo argentino, no campo da medicina social latino-americana. Destaca três dimensões que constituem as suas orientações básicas no campo da saúde: a elaboração de um pensamento sobre o social, sistemático e reflexivo; uma atitude crítica na problematização do ensino e das práticas profissionais; um compromisso com a institucionalização e divulgação do saber sanitário.


Subject(s)
Animals , Anesthetics, General/pharmacology , Luciferases, Firefly/antagonists & inhibitors , Anisotropy , Binding Sites , Biophysical Phenomena , Biophysics , Crystallography, X-Ray , Fatty Alcohols/pharmacology , Halothane/pharmacology , In Vitro Techniques , Luciferases, Firefly/chemistry , Models, Molecular , Protein Conformation , Protein Structure, Tertiary , Thermodynamics
19.
Article in Chinese | WPRIM | ID: wpr-330215

ABSTRACT

To reveal the characterization of interaction between Chinese and western medicinal injections, isothermal titration calorimetry (ITC) was applied to evaluating the interaction of Yiqi Fumai injection (YQFM, as mode drug) with epinephrine hydrochloride injection (YS) and 5% glucose injection (5% GS). The diversification of Gibbs free energy (ΔG), enthalpy (ΔH), and entropy (ΔS) were determined to judge the reaction types of colliquefaction procedures of different injections. Meanwhile, the fingerprints of YQFM before and after combined with the various injections were compared to validate the results. This work demonstrated that during the titration procedure of YQFM and YS, [ΔH] > T [ΔS] , that was to say the reaction was enthalpy-driving. And the reactive profile indicated that a great deal of heat gave out during the procedure. Obviously, chemical reactions happened and the internal component changed. On the other side, the reaction of YQFM combined with 5% GS was entropy-driving, because [ΔH] < T [ΔS]. The reactive profile showed there was only a little heat released. So non-chemical reactions happened and the major ingredients did not change. ITC could be applied to the evaluation on compatibility of other kinds of Chinese and western medicinal injection combination.


Subject(s)
Calorimetry , Drug Interactions , Drugs, Chinese Herbal , Chemistry , Pharmacology , Entropy , Epinephrine , Chemistry , Pharmacology , Glucose , Chemistry , Pharmacology , Injections , Thermodynamics
20.
Article in Chinese | WPRIM | ID: wpr-815239

ABSTRACT

OBJECTIVE@#To explore the interaction between cefdinir (CE) and bovine serum albumin (BSA) by fluorescence and ultraviolet-visible absorption spectrometry.
@*METHODS@#Under the optimal conditions, the interaction between CE and BSA was investigated by fluorescence and ultraviolet-visible absorption spectrometry.
@*RESULTS@#CE could quench (static quenching) the intrinsic fluorescence of BSA by forming the CE-BSA complex. The main binding forces were considered as hydrogen bonds and Van der Waals forces based on the calculated values of the thermodynamic parameter. The process of binding was spontaneous because Gibbs free energy change was negative. The primary binding site for CE was located at sub-domain II of BSA. The values of Hill's coefficients were less than 1, indicating a negative cooperative effect. Synchronous fluorescence spectra showed that the conjugation reaction between CE and BSA did not affect the conformation of BSA, and the binding site was close to the tyrosine residue.
@*CONCLUSION@#This test provides a theoretical basis for revealing the pharmacokinetic issue and the development for new drugs.


Subject(s)
Binding Sites , Cefdinir , Cephalosporins , Chemistry , Serum Albumin, Bovine , Chemistry , Spectrophotometry, Ultraviolet , Thermodynamics
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