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1.
Int. j. morphol ; 34(2): 763-769, June 2016. ilus
Article in English | LILACS | ID: lil-787066

ABSTRACT

The aim of this study was to evaluate the effects of melatonin healing in a tibial bone defect model in rats by means of histopathological and immunohistochemistry analysis. Twenty one male Wistar albino rats were used in this study. In each animal, bone defects (6 mm length ) were created in the tibias. The animals were divided into three groups. In group 1 control group (rats which tibial defects). Group 2 melatonin (10 mg/kg) + 14 days in the tibial defect group) was administered intraperitoneally to rats. Group 3 melatonin (10 mg/kg) + 28 days in the tibial defect group) was administered intraperitoneally to rats. Histopathological analysis of samples was performed to evaluate the process of osteoblastic activity, matrix formation, trabecular bone formation and myeloid tissue in bone defects. Immunohistochemical and immunoblot analysis demonstrated non-collagenous proteins (osteopontin and osteonectin) differences in tibial bone defects. The expression of osteopontin on tibia was increased by 14 days melatonin treatment. The expression of osteonectin on tibia was dramatically increased by 14 days melatonin treatment.


El objetivo fue evaluar por medio de análisis histopatológico e inmunohistoquímico los efectos cicatrizantes de la melatonina en un modelo de defecto óseo tibial en ratas. Se utilizaron 21 ratas albinas Wistar macho. En cada animal, se crearon defectos óseos en las tibias de 6 mm de longitud. Los animales se dividieron en tres grupos. El Grupo 1 correspondió al grupo control (defectos tibiales sin tratamiento). Al Grupo 2 se administró melatonina por vía intraperitoneal (10 mg/kg) 14 días posteriores al defecto tibial. Al Grupo 3 se administró melatonina por vía intraperitoneal (10 mg/kg) 28 días posteriores al defecto tibial. Se realizó un análisis histopatológico para evaluar los procesos de actividad osteoblástica, formación de matriz, formación de hueso trabecular y tejido mieloide en los defectos óseos. Los análisis inmunohistoquímicos y de inmunotransferencia mostraron diferencias de proteínas no colágenas (osteopontina y osteonectina). La expresión de osteopontina en defectos óseos tibiales se incrementó en el Grupo 2. La expresión de osteonectina en la tibia se incrementó fuertemente bajo el tratamiento con melatonina por 14 días.


Subject(s)
Animals , Rats , Melatonin/pharmacology , Tibial Fractures/drug therapy , Tibia/drug effects , Disease Models, Animal , Melatonin/administration & dosage , Osteonectin/drug effects , Osteonectin/metabolism , Osteopontin/drug effects , Osteopontin/metabolism , Rats, Sprague-Dawley , Tibial Fractures/pathology , Tibia/pathology , Wound Healing/drug effects
2.
Clinics ; 70(6): 419-422, 06/2015. tab
Article in English | LILACS | ID: lil-749786

ABSTRACT

OBJECTIVES: Pilon fracture is a complex injury that is often associated with severe soft tissue damage and high rates of surgical site infection. The goal of this study was to analyze and identify independent risk factors for surgical site infection among patients undergoing surgical fixation of a pilon fracture. METHODS: The medical records of all pilon fracture patients who underwent surgical fixation from January 2010 to October 2012 were reviewed to identify those who developed a surgical site infection. Then, we constructed univariate and multivariate logistic regressions to evaluate the independent associations of potential risk factors with surgical site infection in patients undergoing surgical fixation of a pilon fracture. RESULTS: A total of 519 patients were enrolled in the study from January 2010 to October 2012. A total of 12 of the 519 patients developed a surgical site infection, for an incidence of 2.3%. These patients were followed for 12 to 29 months, with an average follow-up period of 19.1 months. In the final regression model, open fracture, elevated postoperative glucose levels (≥125 mg/dL), and a surgery duration of more than 150 minutes were significant risk factors for surgical site infection following surgical fixation of a pilon fracture. CONCLUSIONS: Open fractures, elevated postoperative glucose levels (≥125 mg/dL), and a surgery duration of more than 150 minutes were related to an increased risk for surgical site infection following surgical fixation of a pilon fracture. Patients exhibiting the risk factors identified in this study should be counseled regarding the possible surgical site infection that may develop after surgical fixation. .


Subject(s)
Humans , Young Adult , Fracture Fixation, Internal/adverse effects , Fractures, Open/surgery , Surgical Wound Infection/etiology , Tibial Fractures/surgery , Antibiotic Prophylaxis , Cefazolin/therapeutic use , Follow-Up Studies , Fractures, Open/complications , Hyperglycemia/complications , Medical Records , Multivariate Analysis , Operative Time , Postoperative Care , Retrospective Studies , Risk Factors , Smoking/adverse effects , Tibial Fractures/complications , Tibial Fractures/drug therapy
3.
Acta cir. bras ; 29(12): 794-800, 12/2014. graf
Article in English | LILACS | ID: lil-731029

ABSTRACT

PURPOSE: To evaluate the performance of composites consisting of fragmented cortical bone heteroimplant in association with methylmethacrylate preserved in 98% glycerin, in segmental bone defect of rabbit tibia medial metaphysis. METHODS: In this study were used twelve adult New Zealand rabbits, divided into three groups of four animals each: G30 (30 days), G60 (60 days) and G90 (90 days). The bone defects previously created in the tibia were filled with composites and both were evaluated by cone-beam computed tomography, immediately after surgery and after 30, 60, and 90 days. RESULTS: The composites fulfilled and remained in the sites of bone defects in all cases and were not registered signals of infection, migration or rejection. CONCLUSIONS: The implanted composites promoted the bone defects repair without signals of infection and/or rejection. The composites are one more option for bone defects repair. .


Subject(s)
Animals , Rabbits , Biocompatible Materials/therapeutic use , Bone Substitutes/therapeutic use , Fracture Healing/physiology , Methylmethacrylates , Tibia/surgery , Models, Animal , Postoperative Period , Tomography, X-Ray Computed , Tibia , Tibial Fractures/drug therapy
4.
Rev. cuba. med. mil ; 37(4)oct.-dic. 2008.
Article in Spanish | LILACS | ID: lil-547126

ABSTRACT

Se realizó una revisión bibliográfica y actualización sobre los tratamientos de los pacientes que sufren facturas abiertas de la diáfisis tibial, con el objetivo de facilitar el estudio en este polémico tema a los residentes y especialistas en Traumatología para su manejo. Se profundizó en los conocimientos de aspectos importantes como las clasificaciones y específicamente los factores que las modifican, importancia de la irrigación sanguínea y el desbridamiento quirúrgico. Especial hincapié se realizó en el uso de los antibióticos tanto sistémicos como locales, y estos últimos en combinación con el cemento óseo. Se analizaron los criterios de amputaciones, por ser tan frecuentes en esta entidad, y los métodos de estabilización ósea (fijación externa o interna), por la controversia en sus indicaciones precisas. Se exponen aspectos importantes en el manejo definitivo de las heridas, según el tiempo y tipos de cierres aplicados en las fracturas abiertas por ser muy contaminadas. Todo esto debe incidir en un mejor tratamiento en los pacientes con estas lesiones traumáticas.


A bibliographic review and updating of the treatments of patients with open fractures of the tibial diaphysis was made aimed at making easier the study of this polemic topic for residents and specialists in Traumatology.Wçe went deep into the knowledge of important aspects such as the classifications and, specifically, the factors modifying them, the significance of blood irrigation, and surgical debridement. Special emphasis was given to the use of systemic and local antibiotics and to the combination of the latter with bone cement. The amputation criteria, which are very frequent in this entity were analyzed, as well as the methods of bone stabilization (external or internal fixation), due the controversy on its precise indications. Important aspects on the definite management of wounds are exposed, according to time and to the types of closing applied to open fractures, taking into account their high level of contamination. All this should lead to a better treatment of patients with traumatic lesions.


Subject(s)
Humans , Anti-Bacterial Agents/therapeutic use , Fractures, Open/classification , Fractures, Open/drug therapy , Tibial Fractures/diagnosis , Tibial Fractures/drug therapy
5.
Acta ortop. bras ; 12(4): 197-204, out.-dez. 2004. ilus, tab, graf
Article in English, Portuguese | LILACS | ID: lil-393512

ABSTRACT

Os AINH (Antiinflamatórios não hormonais) são agentes utilizados na prática clínica que interferem no processo inflamatório pela inibicão da síntese de prostaglandinas e tromboxanos. Alguns trabalhos experimentais investigaram sua acão no processo de consolidacão de fraturas, por meio de estudos clínicos e histológicos, sendo escassas as análises biomecânicas. Nesse estudo foram utilizados 20 ratos da linhagem Wistar, divididos aleatoriamente em dois grupos iguais: grupo A (controle) e grupo B (tratado com diclofenaco sódico). Em ambos os grupos foram realizadas fraturas abertas, após perfuracão, na tíbia direita. A administracão da droga foi via intramuscular, dose única diária, por 28 dias. Os animais foram pesados semanalmente. Após o sacrifício as tíbias foram dissecadas, pesadas e submetidas a ensaio biomecânico de flexão analisando-se carga máxima, deformacão e coeficiente de rigidez. Observou-se que no grupo tratado com AINH não houve aumento do peso corpóreo a partir da segunda semana e as tíbias fraturadas foram mais pesadas. Neste grupo o calo ósseo suportou menor carga máxima, apresentando maior deformacão e menor coeficiente de rigidez. Nos animais tratados, o osso não fraturado também se mostrou menos rígido. Concluiu-se, nas condicões estudadas, que o DS alterou o processo de consolidacão e o metabolismo ósseo, levando a retardo na maturacão do calo e menor rigidez do osso intacto, respectivamente.


Subject(s)
Animals , Rats , Diclofenac/adverse effects , Diclofenac/therapeutic use , Tibial Fractures , Anti-Inflammatory Agents , Tibial Fractures/drug therapy
6.
Rev. bras. ortop ; 34(6): 395-400, jun. 1999. ilus
Article in Portuguese | LILACS | ID: lil-360959

ABSTRACT

O objetivo desta pesquisa foi estudar o efeito do tenoxicam, uma droga antiinflamatória não-esteróide (AINE), no processo de consolidação de fraturas em tíbia de ratos. Os animais foram divididos aleatoriamente em três grupos (I, II, III), um dos quais utilizado como controle. No grupo controle (n = 12 animais) foi administrado soro fisiológico a 0,9 por cento por via intramuscular, enquanto no grupo 11, com 12 animais, administraram-se 10 miligramas/quilograma/dia do AINE por via intramuscular; no grupo III (n = 4 animais) foram administrados 20 miligramas/quilograma/dia da droga. O início da administração das substâncias se deu aproximadamente duas horas após a produção da fratura. A formação do calo ósseo foi avaliada histologicamente por microscopia de luz, corando-se as peças com hematoxilina-eosina, picromallory e alcian blue pH 2,5-safranina. O calo ósseo foi estudado histomorfologicamente ao término da pesquisa, segundo escore qualitativo de maturidade. Os resultados indicaram que o tenoxicam induziu alterações histológicas qualitativas, caracterizadas pelo atraso no processo de consolidação óssea. No grupo III ocorreu acentuado retarde da osteogênese, sugerindo ação de dose-dependência da droga. Nas condições estudadas, observou-se que o tenoxicam administrado por via intramuscular retarda o processo de consolidação de fratura de tíbia de ratos em relação direta com a dose empregada.


Subject(s)
Animals , Rats , Anti-Inflammatory Agents, Non-Steroidal , Fracture Healing , Tibial Fractures/drug therapy , Bony Callus , Histological Techniques , Treatment Outcome
7.
Folha méd ; 107(2): 79-82, ago. 1993. tab
Article in Portuguese | LILACS | ID: lil-170340

ABSTRACT

Foram avaliados 30 pacientes com patologias inflamatórias agudas nåo infecciosas do aparelho locomotor. Houve melhora total ou parcial de todas as principais manifestaçöes da inflamaçåo em todos os pacientes estudados. A tolerabilidade foi excelente, e a maioria dos pacientes obteve resposta clínica em até 1 hora após a ingeståo do nimesulide


Subject(s)
Humans , Anti-Inflammatory Agents, Non-Steroidal/therapeutic use , Inflammation/drug therapy , Low Back Pain/drug therapy , Sulfonamides/therapeutic use , Ankle Injuries/drug therapy , Bursitis/drug therapy , Calcaneus/injuries , Finger Injuries/drug therapy , Tibial Fractures/drug therapy , Fractures, Bone/drug therapy , Leg Injuries/drug therapy , Shoulder Fractures/drug therapy , Synovitis/drug therapy , Tennis Elbow/drug therapy , Arm Injuries/drug therapy
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