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1.
Braz. j. med. biol. res ; 54(2): e10099, 2021. tab
Article in English | ColecionaSUS, LILACS, ColecionaSUS | ID: biblio-1142582

ABSTRACT

The objective of this study was to analyze the infection rate and drug resistance of Ureaplasma urealyticum (UU) and Mycoplasma hominis (MH) in the genitourinary tract of Chinese patients. From December 2018 to June 2019, vaginal secretion or urinary secretion of outpatients in our hospital were selected for culture and drug sensitivity analysis of Ureaplasma urealyticum and Mycoplasma hominis. In 4082 Chinese samples, 1567 Mycoplasma were detected, a detection rate of 38.39%, among which 1366 cases were UU single positive, accounting for 33.47%, 15 cases were MH single positive, accounting for 0.36%, 186 cases were UU and MH mixed positive, accounting for 4.56%. The most affected age groups were 21-30 years and 31-40 years, accounting for 19.09 and 15.05%, respectively. The results of drug sensitivity showed that doxycycline, minocycline, josamycin, clarithromycin, and roxithromycin were more sensitive to mycoplasma infection. The distribution of Ureaplasma urealyticum and Mycoplasma hominis in the human genitourinary system and their sensitivity to antibiotics is different for sex and age groups.


Subject(s)
Humans , Male , Female , Adult , Young Adult , Ureaplasma urealyticum/drug effects , Ureaplasma Infections/microbiology , Mycoplasma hominis/drug effects , Microbial Sensitivity Tests , China , Ureaplasma urealyticum/isolation & purification , Mycoplasma hominis/isolation & purification , Asian Continental Ancestry Group , Anti-Bacterial Agents/pharmacology
2.
Rev. chil. obstet. ginecol. (En línea) ; 84(1): 49-54, feb. 2019. tab, graf
Article in Spanish | LILACS | ID: biblio-1003722

ABSTRACT

RESUMEN OBJETIVO: Ureaplasma urealyticum es el agente más frecuentemente aislado en infección intraamniótica. Los macrólidos son los antimicrobianos de primera elección en embarazadas. Se ha descrito el aumento de resistencia, pudiendo limitar las opciones terapéuticas durante la gestación. El propósito del estudio es evaluar susceptibilidad antimicrobiana de Ureaplasma urealyticum aislado en mujeres en edad fértil, que se atienden en Clínica Alemana Temuco, Araucanía, Chile. METODO: Se estudian todas las muestras de orina y flujo vaginal para cultivo de U. urealyticum, de pacientes entre 18 y 40 años, recibidas en el Laboratorio de Microbiología Clínica Alemana Temuco, en período Abril 2013 a Enero 2015. Se procesan las muestras con kit Mycoplasma IST 2 de Biomerieux. En las que resultan positivas, se estudia susceptibilidad a macrólidos, tetraciclinas y quinolonas. RESULTADOS: 426 muestras de orina y flujo vaginal (390 pacientes). 197 pacientes resultaron positivas para U. urealyticum. (50,5%). La susceptibilidad fue 88,4% (174 pctes) a Eritromicina, 87,9% (173 pctes) a Claritromicina y 91,9% (181 pctes) a Azitromicina (NS). 15 de 197 pacientes (7,6%) fueron resistentes a los 3 macrólidos. La susceptibilidad a Quinolonas fue 55,3% a Ciprofloxacino, y 94% a Ofloxacino. El 100% resultó susceptible a Tetraciclinas. CONCLUSIONES: Cerca del 10% de U. urealyticum aislados en nuestra serie son resistentes a macrólidos, contribuyendo a la no erradicación de la infección en tratamientos empíricos. Dentro de ellos, azitromicina aparece con la mayor efectividad. El aumento de resistencia limitará opciones terapéuticas, con gran impacto perinatal en futuro. La vigilancia de susceptibilidad en cada hospital es fundamental para elección terapéutica.


ABSTRACT INTRODUCTION: Ureaplasma urealyticum is the most frequently isolated microorganism in intra-amniotic infection. The macrolides are the first choice antimicrobials for treat this infection in pregnancy. The increasing resistance has been described worldwide, seriously limiting therapeutic options in pregnancy. The aim of the study is to evaluate antimicrobial susceptibility of U. urealyticum aislated in fertile-age women in Clínica Alemana Temuco, Araucania region, Chile. METHOD: Urine and vaginal samples were analyzed for U. urealyticum, from every 18 to 40 years old patients, received at Microbiology Laboratory of Clínica Alemana Temuco, between April 2013 to January 2015. The samples are processed with Mycoplasma IST 2 kit of Biomerieux. If they became positives, susceptibility to macrolides, tetracyclines and quinolones was studied. RESULTS: 426 urine and vaginal samples were collected (390 patients). 197 patients were positive for U. urealyticum (50.5%). The susceptibility was 88.4% (174 pts) to Erythromicyn, 87.9% (173 pts) to Clarithromycin and 91.9% (181 pts) to Azithromycin (NS). Resistance to all macrolides was observed in 15 out of 197 patients (7.6%). The susceptibility to Quinolones was 55.3% to Ciprofloxacin, and 94% to Ofloxacin. The 100% was susceptible to Tetracyclines. DISCUSSION: Near to 10% of isolated Ureaplasma spp in our serie were resistant to some macrolide, being a factor for failing to eradicate the infection in empirical treatment. Azithromycin was the most effective. The increasing resistance will limit therapeutic options, with great perinatal impact in the future. Susceptibility surveillance in each hospital is very important for therapeutic options.


Subject(s)
Humans , Female , Adolescent , Adult , Young Adult , Ureaplasma urealyticum/drug effects , Anti-Bacterial Agents/pharmacology , Tetracycline/pharmacology , Urine/microbiology , Urogenital System/microbiology , Microbial Sensitivity Tests , Erythromycin/pharmacology , Ureaplasma urealyticum/isolation & purification , Azithromycin/pharmacology , Quinolones/pharmacology , Macrolides/pharmacology , Drug Resistance, Bacterial
3.
Braz. j. med. biol. res ; 49(10): e5303, 2016. tab, graf
Article in English | LILACS | ID: lil-792526

ABSTRACT

The shipment and storage conditions of clinical samples pose a major challenge to the detection accuracy of Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), and Ureaplasma urealyticum (UU) when using quantitative real-time polymerase chain reaction (qRT-PCR). The aim of the present study was to explore the influence of storage time at 4°C on the DNA of these pathogens and its effect on their detection by qRT-PCR. CT, NG, and UU positive genital swabs from 70 patients were collected, and DNA of all samples were extracted and divided into eight aliquots. One aliquot was immediately analyzed with qRT-PCR to assess the initial pathogen load, whereas the remaining samples were stored at 4°C and analyzed after 1, 2, 3, 7, 14, 21, and 28 days. No significant differences in CT, NG, and UU DNA loads were observed between baseline (day 0) and the subsequent time points (days 1, 2, 3, 7, 14, 21, and 28) in any of the 70 samples. Although a slight increase in DNA levels was observed at day 28 compared to day 0, paired sample t-test results revealed no significant differences between the mean DNA levels at different time points following storage at 4°C (all P>0.05). Overall, the CT, UU, and NG DNA loads from all genital swab samples were stable at 4°C over a 28-day period.


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Young Adult , Chlamydia trachomatis/genetics , DNA, Bacterial/isolation & purification , Neisseria gonorrhoeae/genetics , Real-Time Polymerase Chain Reaction/methods , Specimen Handling , Ureaplasma urealyticum/genetics , Bacterial Load , Chlamydia trachomatis/isolation & purification , Genitalia/microbiology , Neisseria gonorrhoeae/isolation & purification , Reference Values , Time Factors , Ureaplasma urealyticum/isolation & purification
4.
Article in English | WPRIM | ID: wpr-173131

ABSTRACT

The aim of this study was to determine whether intra-amniotic infection/inflammation (IAI) was associated with subsequent ruptured membranes in women with preterm labor and intact membranes who had a clinically indicated amniocentesis. This retrospective cohort study included 237 consecutive women with preterm labor (20-34.6 weeks) who underwent amniocentesis. The clinical and laboratory parameters evaluated included demographic variables, gestational age, C-reactive protein (CRP) and amniotic fluid (AF) white blood cell, interleukin-6 (IL-6) and culture results. IAI was defined as a positive AF culture and/or an elevated AF IL-6 level (>2.6 ng/mL). The primary outcome was ruptured membranes in the absence of active labor occurring within 48 hours of amniocentesis. Preterm premature rupture of membranes subsequently developed in 10 (4.2%) women within 48 hr of amniocentesis. Multivariate analysis demonstrated that only IAI was independently associated with the ruptured membranes occurring within 48 hr of amniocentesis. In the predictive model based on variables assessed before amniocentesis, only CRP level was retained. IAI is an independent risk factor for subsequent ruptured membranes after clinically indicated amniocentesis in preterm labor. Prior to amniocentesis, measurement of serum CRP level can provide a risk assessment for the subsequent development of ruptured membranes after the procedure.


Subject(s)
Adult , Amniocentesis/adverse effects , Amnion/physiopathology , Amniotic Fluid/cytology , Bacterial Infections/etiology , C-Reactive Protein/analysis , Cohort Studies , Demography , Female , Gestational Age , Humans , Inflammation/etiology , Interleukin-6/metabolism , Leukocytes/cytology , Multivariate Analysis , Mycoplasma/isolation & purification , Obstetric Labor, Premature/etiology , Pregnancy , ROC Curve , Retrospective Studies , Risk Factors , Ureaplasma urealyticum/isolation & purification
5.
Article in English | WPRIM | ID: wpr-80824

ABSTRACT

BACKGROUND: To investigate the risk factors for vaginal infections and antimicrobial susceptibilities of vaginal microorganisms among women who experienced preterm birth (PTB), we compared the prevalence of vaginal microorganisms between women who experienced preterm labor (PTL) without preterm delivery and spontaneous PTB. METHODS: Vaginal swab specimens from 126 pregnant women who experienced PTL were tested for group B streptococcus (GBS), Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma urealyticum, Chlamydia trachomatis, Trichomonas vaginalis, Neisseria gonorrhoeae, Treponema pallidum, herpes simplex virus (HSV) I and II, and bacterial vaginosis. A control group of 91 pregnant women was tested for GBS. Antimicrobial susceptibility tests were performed for GBS, M. hominis, and U. urealyticum. RESULTS: The overall detection rates for each microorganism were: U. urealyticum, 62.7%; M. hominis, 12.7%; GBS, 7.9%; C. trachomatis, 2.4%; and HSV type II, 0.8%. The colonization rate of GBS in control group was 17.6%. The prevalence of GBS, M. hominis, and U. urealyticum in PTL without preterm delivery and spontaneous PTB were 3.8% and 8.7% (relative risk [RR], 2.26), 3.8% and 17.3% (RR, 4.52), and 53.8% and 60.9% (RR, 1.13), respectively, showing no significant difference between the 2 groups. The detection rate of M. hominis by PCR was higher than that by culture method (11.1% vs. 4.0%, P=0.010). The detection rates of U. urealyticum by PCR and culture method were 16.7% and 57.1%, respectively. CONCLUSIONS: There was no significant difference in the prevalence of GBS, M. hominis, and U. urealyticum between the spontaneous PTB and PTL without preterm delivery groups.


Subject(s)
Female , Humans , Microbial Sensitivity Tests , Mycoplasma Infections/complications , Mycoplasma hominis/isolation & purification , Obstetric Labor, Premature/epidemiology , Pregnancy , Pregnancy Complications, Infectious/epidemiology , Premature Birth/epidemiology , Prevalence , Risk Factors , Streptococcal Infections/complications , Streptococcus agalactiae/isolation & purification , Ureaplasma Infections/complications , Ureaplasma urealyticum/isolation & purification , Vagina/microbiology
6.
Braz. j. microbiol ; 42(1): 256-260, Jan.-Mar. 2011. ilus
Article in English | LILACS | ID: lil-571397

ABSTRACT

The study was carried out on 1068 infertile women under initial evaluation. For Mycoplasma hominis, the highest resistance rates were registered for ciprofloxacin (72.22 percent), followed by macrolides and ofloxacin. For Ureaplasma urealyticum, the ciprofloxacin resistance was also high (51.72 percent), while the resistance rates to other tested antibiotics were significantly lower.


Subject(s)
Humans , Female , Fluoroquinolones/isolation & purification , In Vitro Techniques , Infertility, Female , Mycoplasma hominis/isolation & purification , Ureaplasma urealyticum/isolation & purification , Methods , Reference Parameters , Methods , Women
7.
Indian J Med Microbiol ; 2011 Jan-Mar; 29(1): 33-36
Article in English | IMSEAR | ID: sea-143772

ABSTRACT

Objectives: The aim of this investigation was to simultaneously detect and differentiate Mycoplasma genitalium and Ureaplasma urealyticum in female patients suffering from genital complications by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). Materials and Methods : Genital swabs were taken from 210 patients. They were transported to the laboratory in phosphate-buffered saline. For PCR, samples were analysed with genus-specific MyUu-R and MyUu-F primers. This primer set, which was originally designed in our laboratory, amplified a 465 bp fragment (M. genitalium) and a 559 bp fragment (U. urealyticum). Samples containing a band of the expected sizes for the Mycoplasma strains were subjected to digestion with a restriction endonuclease enzyme of TaqI and Cac8I. Results: Of the 210 samples, a total of 100 (47.6%) samples were found to be positive for Mycoplasmas (seven M. genitalium isolates, 3.3%; and 89 U. urealyticum isolates, 42.4%), and coinfections with both species were detected in four samples (1.9%). The PCR-RFLP results showed that M. genitalium and U. urealyticum are different by enzyme patterns. Conclusion: PCR-RFLP offers a rapid and easily applicable protocol to simultaneous detection and differentiation of M. genitalium and U. urealyticum from clinical samples when specific primers and restriction enzymes are used.


Subject(s)
Adult , Aged , Bacteriological Techniques/methods , Diagnosis, Differential , Female , Genitalia, Female/microbiology , Humans , Middle Aged , Mycoplasma Infections/diagnosis , Mycoplasma Infections/microbiology , Mycoplasma genitalium/classification , Mycoplasma genitalium/genetics , Mycoplasma genitalium/isolation & purification , Polymerase Chain Reaction/methods , Polymorphism, Restriction Fragment Length , Pregnancy , Pregnancy Complications, Infectious/diagnosis , Pregnancy Complications, Infectious/microbiology , Time Factors , Ureaplasma Infections/diagnosis , Ureaplasma Infections/microbiology , Ureaplasma urealyticum/classification , Ureaplasma urealyticum/genetics , Ureaplasma urealyticum/isolation & purification
8.
Behbood Journal. 2009; 13 (3): 197-202
in Persian | IMEMR | ID: emr-129546

ABSTRACT

Mycoplasma hominis and Ureaplasma urealyticum microorganisms may play a role in infertility. The aim of this study is to determine prevalence of Mycoplasma hominis and Ureaplasma urealyticum microorganisms in infertile women and their husbands. In this Cross-sectional study presence of Mycoplasma hominis and Ureaplasma urealyticum were examined in infertile couples who have been referred to infertility clinics [Avicenna Institute in Tehran] by gynecologists. Referred patients with cervical smear and their husbands with semen fluid culture were asked to fill in a questionnaire. The samples were sent to microbiology laboratories. Data were analyzed using SPSS and Chi-square, Mann-Whitney and T-test methods. 56 infertile women and their husbands were studied and compared. The average age in subjects was 30.41 year and their marriage age was 22.96 year. Prevalence of Mycoplasma hominis in women was 27.39% [22 out of 56] and in their husbands was 19.64% [11]. Prevalence of Ureaplasma urealyticum in women was 58.92% [33] and in their husbands was 46.42% [26]. There was no statistically significant differences between the infection and patient age, marriage age, educational levels and infertility duration. This study showed a high prevalence of mycoplasma hominis and ureaplasma urealyticum infection in both infertile women and their husbands. However, the prevalence of infections in women was higher than their husbands. We concluded that treatment of infected women is necessary and further studies are required to be done to better understand the role of these infections in infertility of women


Subject(s)
Humans , Male , Female , Mycoplasma hominis/isolation & purification , Ureaplasma urealyticum/isolation & purification , Prevalence , Cross-Sectional Studies , Spouses
9.
Article in English | IMSEAR | ID: sea-23651

ABSTRACT

BACKGROUND & OBJECTIVE: Ureaplasma urealyticum has been implicated in various neonatal morbidities in preterm infants. Its association with chronic lung disease (CLD) remains controversial. The aim of this prospective study was to investigate colonization of U. urealyticum in preterm infants (with gestational age <34 wk) and to evaluate the relationship between U. urealyticum colonization and neonatal morbidity including CLD. METHODS: U. urealyticum was cultured from nasopharyngeal or endotracheal aspirates collected within 24 h of birth from infant <or=34 wk gestation weighing <1800 g admitted to a Neonatal Intensive Care Unit of a tertiary care hospital in north India, and PCR was performed on the DNA extracted from these samples. RESULTS: Twenty per cent of the study infants were colonized with U. urealyticum. The mean gestational age of the infants in the colonized group was less than that of non colonized infants (P<0.05). The peripheral total leukocyte counts and mortality rate were higher in infants with U. urealyticum colonization than in non-colonized infants (P<0.05). There was no significant difference between the colonized and non colonized groups with regard to the antenatal use of steroids, sex, cause of respiratory distress, use of surfactant, duration of ventilation. INTERPRETATION & CONCLUSION: None of the 20 babies colonized with U. urealyticum developed CLD as compared with two (2.5%) of the non colonized group. Colonization of the airways with U. urealyticum had no significant role in development of CLD in Indian preterm infants.


Subject(s)
Female , Humans , Infant, Newborn , Infant, Premature , Longitudinal Studies , Male , Nasopharynx/microbiology , Polymerase Chain Reaction , Prospective Studies , Trachea/microbiology , Ureaplasma urealyticum/isolation & purification
10.
Scientific Journal of Al-Azhar Medical Faculty [Girls][The]. 2005; 26 (1): 479-487
in English | IMEMR | ID: emr-112393

ABSTRACT

The main feature of BV is a change in vaginal flora from predominant Lactobacilli to overgrowth of other microorganisms like; Mycoplasma hominis [M. hominis] and Ureaplasma urealyticum [U. urealyticum]. M. hominis and U. urealyticum vaginosis has been associated with abortion, intraamniotic infection, premature rupture of membranes [PROM] and intrapartum fever in pregnant women. This study was carried out on 100 pregnant women complaining of abnormal vaginal discharge in addition, 50 normal healthy pregnant women were included as a control. Vaginal specimens were collected from all studied women. The incidence of BV among pregnant women was [39%] whereas none of the controls has BV. There was positive correlation between the number of pregnancy but not its duration. M. hominis was detected in BV cases [38.5%] and U. urealyticum in [46.2%]. As regarding the susceptibility, M. hominis was more sensitive to norfioxacin and tetracycline [100% for each] and also, U. urealyticum isolates were more sensitive to norfioxacin and tetracycline [94.4, 88.9%] respectively, but they were resistant to Amoxicillin and Amoxacillin/Culvunate


Subject(s)
Humans , Female , Mycoplasma hominis/isolation & purification , Ureaplasma urealyticum/isolation & purification , Pregnant Women , Vaginal Smears/microbiology , Vaginal Discharge
11.
Acta bioquím. clín. latinoam ; 34(3): 331-7, sept. 2000. ilus
Article in Spanish | LILACS | ID: lil-288918

ABSTRACT

Chalamydia trachomatis, Ureaplasma urealyticum y Mycoplasma hominis son microorganismos responsables de infecciones urogenitales. Son aislados con considerable frecuencia del tracto genital femenino. En este trabajo se estudiaron 100 exudados vaginales de mujeres promiscuas que concurrieron a la división de Bacteriología del Hospital Central de Río Cuarto. En todas las muestras se investigó la presencia de C. trachomatis, U. urealyticum, M. hominis. La prevalencia hallada fue: C. trachomatis 17 por ciento; U. urealyticum 57 por ciento; M. hominis 21 por ciento y Neisseria gonorrhoeae 2 por ciento. Las asociaciones más frecuentes fueron: C. trachomatis-Trichomonas vaginalis, micoplasmas-T. vaginalis y Gardnerella vaginalis-Candida albicans con un 18 por ciento, 15 por ciento y 8 por ciento respectivamente


Subject(s)
Humans , Female , Adolescent , Adult , Middle Aged , Chlamydia Infections/epidemiology , Mycoplasma Infections/epidemiology , Ureaplasma Infections/epidemiology , Chlamydia Infections/complications , Chlamydia Infections/transmission , Chlamydia trachomatis/isolation & purification , Mycoplasma hominis/isolation & purification , Mycoplasma Infections/complications , Mycoplasma Infections/transmission , Neisseria gonorrhoeae/isolation & purification , Sexually Transmitted Diseases/epidemiology , Sexually Transmitted Diseases/etiology , Ureaplasma Infections/complications , Ureaplasma Infections/transmission , Ureaplasma urealyticum/isolation & purification
12.
Rev. méd. Chile ; 128(9): 985-95, sept. 2000. tab
Article in Spanish | LILACS | ID: lil-274631

ABSTRACT

Background: The prevalence of idiopathic spontaneous premature labor or without an evident clinical cause, has not been reduced with tocolytic treatments, suggesting that premature labor has multiple causes and infections play a not well-defined role. Aim: To perform microbiological studies of the amniotic fluid and of the lower genital tract in women with idiopathic premature labor and intact membranes, relating these findings with maternal and neonatal outcomes. Patients and methods: Women with pregnancies between 24 and 34 weeks, with premature labor and without an evident clinical cause were enrolled. Amniotic fluid and genital tract samples were obtained for traditional microbiological cultures. This information was related with delivery events and neonatal outcome. Results: Sixty-three patients were included. The overall frequency of microbial invasion of amniotic cavity was 23.8 percent and of cervical or vaginal infection was 63.5 percent (in 39.7 percent there was only cervical or vaginal infection without involvement of the amniotic sac). Absence of infection was documented in 36.5 percent of women. Compared to patients without infection, women with microbial invasion of amniotic cavity had a higher rate of prematurity (73.3 percent p < 0.05), a higher rate of prematurity of less than 34 weeks (60 percent p < 0.01), a higher frequency of preterm rupture of membranes (40 percent p < 0.001), a shorter admission-to-delivery interval (median 3.0 days p < 0.01) and lower gestational age at delivery (median 33 weeks p < 0.01). Clinical chorioamnionitis and endometritis (20 percent p < 0.01) was observed only in patients with amniotic cavity infections. Severe asphyxia (26.7 percent p < 0.05) and neonatal admission to Intensive Care Units (46.7 percent p < 0.05) were more frequent and neonatal weight was less in the offspring of women with microbial invasion of amniotic cavity (2020 g median p < 0.01). Conclusions: In preterm labor with intact membranes, intraamniotic infection is the most frequent cause of prematurity and is associated with a higher prevalence of maternal and neonatal problems


Subject(s)
Humans , Female , Pregnancy , Infant, Newborn , Adult , Amniotic Fluid/microbiology , Obstetric Labor, Premature/etiology , Vaginosis, Bacterial/diagnosis , Gardnerella vaginalis/isolation & purification , Obstetric Labor, Premature/diagnosis , Pregnancy Complications, Infectious/microbiology , Pregnancy Trimester, Second , Pregnancy Trimester, Third , Ureaplasma Infections/diagnosis , Ureaplasma urealyticum/isolation & purification , Vagina/microbiology , Vaginosis, Bacterial/drug therapy
13.
Rev. Inst. Med. Trop. Säo Paulo ; 42(4): 185-8, July-Aug. 2000. ilus, tab
Article in English | LILACS | ID: lil-266050

ABSTRACT

M. hominis and U. urealyticum are the better-known mycoplasma species pathogenic to the human genitourinary tract, causing mainly urethritis, bacterial vaginosis and pregnancy complications. In HIV-infected patients, the prevalence and role of these species is still not well known. The aim of this work was to determinate the prevalence of these species in this group of male patients (HIV group), in comparison to a group of men with clinical symptoms of urethritis (STD group). M. hominis was isolated from 7.5 per cent patients (8/106) and U. urealyticum from 18.9 per cent patients (20/106) from the HIV group, being among these 62.5 per cent and 85 per cent in significant concentrations, respectively. In the STD group these rates were 0.9 per cent (1/110) for M. hominis and 13.6 per cent (15/110) for U. urealyticum, being 100 per cent and 93.3 per cent in significant concentrations, respectively. We could demonstrate infection rates by these mycoplasma species in the HIV group as high as the one found in the STD one, what may indicate the occurrence of opportunistic infections in our population. This fact is discussed here because in immunosuppressed patients, specially M. hominis has been reported causing severe infections, even systemically.


Subject(s)
Humans , Male , Adult , AIDS-Related Opportunistic Infections/epidemiology , HIV-1 , Mycoplasma hominis/isolation & purification , Mycoplasmatales Infections/epidemiology , Ureaplasma urealyticum/isolation & purification , Urethritis/microbiology , Prevalence , Sexually Transmitted Diseases, Bacterial/epidemiology , Urethra/microbiology
15.
Rev. chil. infectol ; 17(1): 7-17, 2000. ilus
Article in Spanish | LILACS | ID: lil-263517

ABSTRACT

Esta revisión define parto prematuro e infección intrauterina (origen, vías y estadios). Resumimos las asociaciones epidemiológicas entre parto prematuro y los diferentes microorganismos. Se discute la interacción entre productos bacterianos y el sistema monocitomacrófago del huésped, los atributos de las citoquinas proinflamatorias y el papel desempeñado en el mecanismo del parto y el síndrome de respuesta inmune fetal


Subject(s)
Humans , Female , Pregnancy , Infant, Newborn , Obstetric Labor, Premature/etiology , Uterine Diseases/microbiology , Vaginosis, Bacterial/complications , Chlamydia trachomatis/isolation & purification , Chlamydia trachomatis/pathogenicity , Cytokines , Mycoplasma hominis/isolation & purification , Mycoplasma hominis/pathogenicity , Neisseria gonorrhoeae/isolation & purification , Neisseria gonorrhoeae/pathogenicity , Oropharynx/microbiology , Streptococcus agalactiae/isolation & purification , Streptococcus agalactiae/pathogenicity , Trichomonas vaginalis/isolation & purification , Trichomonas vaginalis/pathogenicity , Ureaplasma urealyticum/isolation & purification , Ureaplasma urealyticum/pathogenicity
16.
Rev. chil. infectol ; 17(supl.1): 66-71, 2000. tab, graf
Article in Spanish | LILACS | ID: lil-269446

ABSTRACT

La etiología de la uretitis no gonocóccica trasciende las infecciones por chlamydia trachomatis y ureaplasma urealyticum. otros agentes etiológicos comunes son responsables de aproximadamente 20 por ciento de los casos y en un tercio no se precisa una etiología. Por esta situación se requiere de una nueva terminología para caracterizar mejor esta entidad. Test de amplificación genómica aplicados a la secreción uretral u orina son nuevas herramientas para un diagnóstico precoz de chlamydia trachomatis. El diagnóstico precoz es muy importante para evitar complicaciones y secuelas, especialmente infertilidad en mujeres, mediante un tratamiento oportuno y adecuado. Azitromicina, 1g oral, en dosis única es un avance significativo en el tratamiento de la uretritis causada por clamidias


Subject(s)
Humans , Chlamydia trachomatis/isolation & purification , Ureaplasma urealyticum/isolation & purification , Urethritis/diagnosis , Azithromycin/therapeutic use , Chlamydia trachomatis/drug effects , Chlamydia trachomatis/pathogenicity , Gene Amplification , Ureaplasma urealyticum/drug effects , Ureaplasma urealyticum/pathogenicity , Urethritis/drug therapy , Urethritis/etiology , Urine/microbiology
17.
Rev. chil. infectol ; 16(2): 105-11, 1999. ilus, tab
Article in Spanish | LILACS | ID: lil-257960

ABSTRACT

Se ha demostrado una importante asociación entre U. urealyticum y enfermedades materno-infantiles tales como rotura prematura de membranas, parto prematuro y enfermedad respiratoria en niños de muy bajo peso. Debido a que el cultivo de este microorganismo es un procedimiento costoso que requiere de hasta 5 días para descartar una muestra como negativa, se evaluó la sensibilidad y especificidad de la RPC para detectar U. urealyticum en líquido anmiótico. Los resultados indican que la RPC posee alta especificidad (100 por ciento) y sensibilidad (95,8 por ciento). Aunque se requiere de mayor evaluación clínica, este método rápido de detección de la bacteria (menos de 24 horas) es de particular importancia en el manejo y prevención de la morbimortalidad en recién nacidos y embarazadas


Subject(s)
Humans , Female , Pregnancy , Culture Media , Polymerase Chain Reaction , Ureaplasma urealyticum/isolation & purification , Fetal Membranes, Premature Rupture/diagnosis , Fetal Membranes, Premature Rupture/genetics , Fetal Membranes, Premature Rupture/microbiology , Amniotic Fluid/microbiology , Maternal-Fetal Exchange , Sensitivity and Specificity
18.
Rev. argent. microbiol ; 30(2): 53-8, abr.-jun. 1998. tab
Article in Spanish | LILACS | ID: lil-223475

ABSTRACT

Mycoplasma homonis y Ureaplasma urealyticum están estrechamente relacionadas con enfermedades del tracto urogenital como pielonefritis, uretritis no-gonocóccica, cálculos urinarios, epididimitis, inflamaciones pélvicas, infertilidad, abortos y fiebre post-parto; en recién nacidos también pueden causar neumonías y meningitis. Estas bacterias pueden ser diagnosticadas por diferentes métodos. En este trabajo utilizamos la hibridación de ácidos nucléicos y la reacción en cadena de la polimerasa para analizar 22 muestras de pacientes con diferentes síntomas urogenitales, en busca de micoplasmas y ureaplasmas. Como resultado obtuvimos 10 muestras positivas y 12 negativas. Entre las muestras positivas se identificaron 2 como Mycoplasma hominis, 2 como Ureaplasma urealyticum y 6 con ambas especies. los resultados obtenidos por las técnicas moleculares fueron comparados con los métodos de referencia, encontrándose en 18 muestras resultados coincidentes, mientras que en 4 los resultados fueron discordantes, siendo esta diferencia estadísticamente no significativa


Subject(s)
Humans , Molecular Biology , Mycoplasma hominis/isolation & purification , Nucleic Acid Hybridization , Polymerase Chain Reaction , Tenericutes/isolation & purification , Tenericutes/pathogenicity , Ureaplasma urealyticum/isolation & purification , Cuba
19.
Rev. Inst. Med. Trop. Säo Paulo ; 40(1): 1-5, Jan.-Feb. 1998. ilus
Article in English | LILACS | ID: lil-216099

ABSTRACT

O objetivo do presente estudo foi determinar a prevalência de determinadas espécies de micoplasmas, tais como, Mycoplasma hominis, Ureaplasma urealyticum e Mycoplasma penetrans, em swabs uretrais de pacientes infectados com HIV-1 comparando com um grupo controle. Micoplasmas foram detectados por técnicas padräo de cultivo e pela reacäo de polimerase em cadeia para a qual foram utilizados "primers" genericos obtidos da regiäo conservada 16sRNA e "primers" nos dois metodos foi comparavel. Contudo, o PCR mostrou ser mais sensivel nas condicöes empregadas enquanto que o cultivo permitiu a quantificacäo dos isolados. Os resultados demonstraram näo haver diferenças significantes (p<0,05) nas taxas de positividade entre os metodos empregados para a detecçäo dos micoplasmas


Subject(s)
Humans , Male , Urethral Diseases/etiology , AIDS-Related Opportunistic Infections/physiopathology , Polymerase Chain Reaction , Blotting, Western , Endopeptidase K , Enzyme-Linked Immunosorbent Assay , Mycoplasma hominis/isolation & purification , Mycoplasma penetrans/isolation & purification , Serologic Tests/methods , Ureaplasma urealyticum/isolation & purification
20.
Rev. chil. obstet. ginecol ; 63(2): 95-9, 1998. ilus, tab
Article in Spanish | LILACS | ID: lil-231583

ABSTRACT

Se realizaron PCR y cultivos en líquido amniótico (LA) obtenido por amniocenteáis en 50 embarazadas con trabajo de parto prematuro sin rotura de membranas, y en 13 pacientes con embarazo de término, sin trabajo de parto, en quienes se realizó amniocentesis para evaluar madurez pulmonar fetal antes de la interrupción electiva de la gestación. Las pacientes fueron controladas hasta la resolución del embarazo. Se determinó el valor diagnóstico de los exámenes para predecir parto prematuro y complicaciones neonatales. PCR fue positivo en 23 casos, siendo E coli el germen más frecuentemente detectado (13). El cultivo fue positivo en 6 casos, coincidiendo el germen con PCR en 4 de ellos. La evolución a parto prematuro fue diferente entre los grupos PCR + y PCR-. PCR demostró sensibilidad mayor que los cultivos (40 vs 13 por ciento), y especificidad menor (45 vs 90 por ciento) para predecir parto prematuro menor a 37 semanas. La sensibilidad y especificidad para predecir parto prematuro menor a 34 semanas resultó aún mayor (64 y 59 por ciento, respectivamente). Ninguna de las 13 pacientes de término, candidatas a operación cesárea electiva, resultó positiva para PCR o cultivo


Subject(s)
Humans , Pregnancy , Female , Adult , Genes, Bacterial/immunology , Amniotic Fluid/microbiology , Polymerase Chain Reaction , Gene Amplification/methods , Escherichia coli/isolation & purification , Fusobacterium nucleatum/isolation & purification , Haemophilus influenzae/isolation & purification , Obstetric Labor, Premature/diagnosis , Staphylococcus aureus/isolation & purification , Ureaplasma urealyticum/isolation & purification
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