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Braz. j. med. biol. res ; 51(9): e7627, 2018. graf
Article in English | LILACS | ID: biblio-951764


Uterine leiomyomas (ULs) are benign monoclonal tumors that arise from the underlying myometrial tissue in the uterus. Effective therapies are still lacking because of poor understanding of the pathophysiology and epidemiology. Hence, it is urgent to establish efficient animal models to screen novel anti-UL therapies. In this study, for the first time, traditional Chinese medicine and Western medicine were combined to establish an animal model of ULs in rats. In order to evaluate the function and value of the novel model, it was compared with other models. The long-term and short-term rat models for ULs were established using progesterone and diethylstilbestrol. Rats in Qi stagnation and blood stasis group were injected with epinephrine hydrochloride and received chronic unpredictable stress for two weeks. Rats in combining disease with syndrome group (CDWSG) received not only epinephrine hydrochloride injection and chronic unpredictable stress but also progesterone and diethylstilbestrol treatment. We analyzed differences in organ coefficient, uterus size, uterine pathology, concentrations of progesterone, estradiol, progesterone receptor, estrogen receptor, expression of desmin, α-smooth muscle actin, and vimentin among the five groups. The animal model of ULs was successfully constructed by loading the rats with estrogen and progesterone. The rat model of CDWSG was more stable than other groups and the method was the most efficient.

Animals , Female , Rats , Uterine Neoplasms/chemically induced , Disease Models, Animal , Leiomyoma/chemically induced , Medicine, Chinese Traditional , Progesterone/administration & dosage , Enzyme-Linked Immunosorbent Assay , Immunohistochemistry , Epinephrine/administration & dosage , Rats, Sprague-Dawley , Diethylstilbestrol/administration & dosage
Article in English | WPRIM | ID: wpr-147187


The anticarcinogenic effects and mechanisms of the biotechnological drugs of Panax ginseng C.A. Meyer cultivated in Russia, bioginseng, panaxel and panaxel- 5, were studied. Bioginseng was produced from a tissue culture of ginseng root cultured on standard medium, whereas panaxel and panaxel-5 were produced from ginseng tissue root cultures using standard mediums enriched with 2-carboxyethylgermanium sesquioxide and 1-hydroxygermatran-monohydrate respectively. All three ginseng drugs inhibited the development of mammary tumors induced by intramammary injections of N-methyl-N-nitrosourea (MNU) in rats, the development of the brain and spinal cord tumors induced by transplacental administration of N-ethyl-N-nitrosourea (ENU) in rats, and the development of uterine, cervical and vaginal tumors induced by intravaginal applications of 7,12-dimethylbenz(a)anthracene (DMBA) in mice. The ginseng drugs induced the cytotoxic activity of macrophages in mice, enhanced T-lymphocyte rosette formation in guinea pigs exposed to cyclophosphamide, and stimulated the production of thyroid hormones in rats. These mechanisms may contribute to the anticarcinogenic action of the ginseng drugs. The organic germanium compounds present in panaxel and panaxel-5 did not potentiate the anticarcinogenic or immuno- stimulatory effects as much as biogeinseng. Preliminary clinical trials with panaxel and bioginseng were carried out in patients with precancerous lesions of the esophagus and endometrium. Panaxel was found to have a strong therapeutic effect in patients suffering from chronic erosive esophagitis. Bioginseng induced the regression of adenomatous-cystic hyperplasia of the endometrium in some patients. Thus, we conclude that the drugs of ginseng appear to hold considerable promise for future cancer chemoprevention.

Adenocarcinoma/chemically induced , Adult , Animals , Antineoplastic Agents, Phytogenic/therapeutic use , Cells, Cultured , Uterine Cervical Neoplasms/chemically induced , Clinical Trials as Topic , Cytotoxicity Tests, Immunologic , Disease Models, Animal , Endometrial Neoplasms/pathology , Endometrium/pathology , Esophageal Neoplasms/pathology , Esophagus/pathology , Estradiol/blood , Female , Fibroadenoma/chemically induced , Humans , Macrophages, Peritoneal/cytology , Male , Mammary Neoplasms, Experimental/chemically induced , Mice , Mice, Inbred C57BL , Neoplasms, Experimental/chemically induced , Nervous System Neoplasms/chemically induced , Panax/metabolism , Precancerous Conditions/pathology , Rats , Culture Techniques , Uterine Neoplasms/chemically induced , Vaginal Neoplasms/chemically induced
Rev. Hosp. Clin. Fac. Med. Univ. Säo Paulo ; 55(1): 17-20, Jan.-Feb. 2000. ilus
Article in English | LILACS | ID: lil-260703


Müllerian adenosarcoma with sarcomatous overgrowth presented by a 52-year-old female patient after adjuvant tamoxifen treatment for breast carcinoma is described. The diagnosis was made on histological basis after curettage and complementary total hysterectomy with bilateral salpingo-oophorectomy. The immunohistochemical study showed high expression of estrogen receptors in the epithelial component of the lesion and irregularly positive findings in the stroma. The proliferative activity evaluated by Ki-67 immunoexpression was higher in the stroma than the epithelium. Some of the stromal cells showed rhabdomyoblastic differentiation. The association of tamoxifen use and development of mesenchymal neoplasms is discussed.

Humans , Female , Middle Aged , Adenosarcoma/chemically induced , Antineoplastic Agents, Hormonal/adverse effects , Breast Neoplasms/drug therapy , Tamoxifen/adverse effects , Uterine Neoplasms/chemically induced , Adenosarcoma/pathology , Endometrial Neoplasms/chemically induced , Endometrial Neoplasms/pathology , Endometrium/pathology , Hysterectomy , Immunohistochemistry , Uterine Neoplasms/pathology