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1.
Int. j. morphol ; 38(2): 492-498, abr. 2020. graf
Article in Spanish | LILACS (Americas) | ID: biblio-1056467

ABSTRACT

Para promover la reparación y regeneración muscular, se han desarrollado diferentes estrategias, sin embargo, aún no existe un tratamiento único aceptado. Investigaciones relacionan los efectos terapéuticos de la miel en el proceso de cicatrización de heridas de diversas etiologías. Considerando lo anterior, el propósito de este estudio fue evaluar los efectos de una formulación basada en miel nativa (Ulmoplus®) en la reparación de lesiones musculares por incisión quirúrgica. A cinco conejos (Oryctolagus cuniculus) se les realizó una incisión de 20 mm de largo y 3 mm de ancho en la región anterior de cada pierna, atravesando la epidermis, dermis y el músculo tibial anterior, hasta alcanzar el periostio de la tibia. La pierna izquierda de cada animal fue clasificada como grupo sutura (S) y la derecha como grupo sutura más Ulmoplus® (S+U). Posteriormente, al grupo S+U se le aplicó Ulmoplus® en toda la extensión de la herida. Luego, en ambos grupos, la incisión fue suturada con cuatro puntos intradérmicos (vicryl 2/0). Un animal fue considerado como control para obtener imágenes histológicas del músculo tibial anterior sin injuria. A los 10 días post incisión, fueron tomadas las biopsias del músculo y posteriormente fueron procesadas para su observación en microscopio de luz. El estudio se llevó a cabo considerando las recomendaciones de la Guía para el Cuidado y Uso de Animales de Laboratorio. A los 10 días post incisión, las injurias, en ambos grupos de animales, se encontraban cerradas y sin signos de infección. El músculo tibial anterior de los especímenes del grupo S se encontraba en la fase de reparación, con procesos concomitantes de fagocitosis del tejido necrotizado, regeneración de miofibras y formación de tejido conectivo cicatrizal. El músculo tibial anterior de los animales del grupo S+U se encontraba en la fase de remodelación, ya que la arquitectura muscular se evidenciaba restablecida. Ulmoplus®, como coadyuvante en el proceso de cierre de heridas quirúrgicas de músculo esquelético utilizando sutura convencional, es una buena alternativa, ya que acelera el proceso de reparación muscular, y reduce el tejido conectivo cicatrizal.


To promote muscle repair and regeneration, different strategies have been developed, however, there is still no single accepted treatment. Research relates the therapeutic effects of honey in the wound healing process of various etiologies. The aim of this study was to evaluate the effects of native honey formulation (Ulmoplus®) in the repair of muscle injuries by surgical incision. In five rabbits (Oryctolagus cuniculus) were made an incision 20 mm long and 3 mm wide in the anterior region of each leg, crossing the epidermis, dermis and anterior tibial muscle, until reaching the tibia periosteum. The left leg of each animal was classified as a suture group (S) and the right leg as a suture group plus Ulmoplus® (S + U). Subsequently, Ulmoplus® was applied to the entire S + U group throughout the entire length of the wound. Then, in both groups, the incision was sutured with four intradermal points (vicryl 2/0). An animal was considered as a control to obtain histological images of the anterior tibial muscle without injury. At 10 days post incision, muscle biopsies were taken and subsequently processed for observation under light microscopy. The study was carried out considering the recommendations of the Guide for the Care and Use of Laboratory Animals. At 10 days post incision, the injuries, in both groups of animals, were closed and without signs of infection. The anterior tibial muscle of the S group specimens was in the repair phase, with concomitant processes of phagocytosis of the necrotized tissue, myofiber regeneration and formation of scar connective tissue. The anterior tibial muscle of the animals of the S + U group was in the remodeling phase, since the muscular architecture was evidenced restored. Ulmoplus®, as an adjunct in the process of closing surgical wounds of skeletal muscle using conventional suture, is a good alternative, since it accelerates the muscle repair process and reduces scar connective tissue.


Subject(s)
Animals , Rabbits , Wound Healing/drug effects , Surgical Wound/drug therapy , Honey , Muscles/injuries , Sutures , Disease Models, Animal
2.
Int. j. morphol ; 37(3): 1073-1078, Sept. 2019. graf
Article in Spanish | LILACS (Americas) | ID: biblio-1012398

ABSTRACT

Considerando los avances en las investigaciones que relacionan los efectos terapéuticos de la miel en el proceso de cicatrización de heridas, la presente investigación tuvo como objetivo evaluar y comparar cualitativamente, a través de una análisis morfológico, la respuesta tisular local entre sutura convencional coadyuvada con Ulmoplus® y sin Ulmoplus® en el cierre de la incisión de piel de conejo. Se utilizaron 10 conejos adultos, divididos en grupo S (sutura) y grupo S+U (sutura más Ulmoplus®). Se les realizó una incisión de 10 mm de largo, en la región anterior de la pierna, a través de la epidermis y dermis, hasta llegar a la fascia profunda que cubría al músculo tibial anterior. Posteriormente, solo al grupo S+U se le aplicó Ulmoplus®. Luego, en ambos grupos, la incisión fue suturada con dos puntos intradérmicos de ácido poliglicólico (vicryl 2/0). Después de 10 días post incisión fueron tomadas las biopsias de la piel y procesadas para su observación en microscopio de luz. El estudio se llevó a cabo considerando las recomendaciones de la Guía para el Cuidado y Uso de Animales de Laboratorio. Ambos grupos presentaron una incisión cerrada al día 10, con características microscópicas propias de una fase proliferativa. No obstante, el grupo S+U mostró una cicatriz de menor longitud y de aspecto más plano, cuya epidermis estaba mejor organizada y era de menor grosor, con células basales diferenciadas. A nivel dérmico se observó contracción de la herida, evidenciándose una zona cicatrizal de menor tamaño. Ulmoplus®, es una buena alternativa como coadyuvante en el proceso de cierre de heridas quirúrgicas utilizando sutura convencional, ya que acelera el proceso de cicatrización y permite mejores resultados estéticos de la cicatriz.


Considering the advances in research that relate honey therapeutic effects in wound healing process, the present investigation aimed to evaluate and compare qualitatively, through morphological analysis, the local tissue response between conventional suture with Ulmoplus ® and without Ulmoplus ® in the closure of the rabbit skin incision. We used 10 adult rabbits, divided into group S (suture) and group S + U (suture plus Ulmoplus®). An 10 mm long incision was made in the anterior region of the leg, passing through epidermis and dermis, until reaching the deep fascia that covered the anterior tibial muscle. Subsequently, only the S + U group was given Ulmoplus®. Then, in both groups, the incision was sutured with two intradermal points of polyglycolic acid (vicryl 2/0). After 10 days post incision, biopsies of the skin were taken and processed for observation in a light microscope. The study was carried out considering the recommendations of the Guide for the Care and Use of Laboratory Animals. Both groups had a closed incision at day 10 with microscopic characteristics of a proliferative phase. However, the S + U group showed a scar of shorter length and flatter appearance, whose epidermis was more organized and thinner, with differentiated basal cells. At the dermal level, wound contraction was observed, evidencing a smaller scar area. Ulmoplus®, as a coadjuvant in closing surgical wounds process using conventional suture is a good alternative, since it accelerates the healing process and allows better aesthetic results of the scar.


Subject(s)
Animals , Rabbits , Skin/drug effects , Wound Healing/drug effects , Suture Techniques , Honey , Skin/ultrastructure , Wound Closure Techniques , Dermatologic Surgical Procedures , Surgical Wound
3.
An. bras. dermatol ; 94(2): 204-210, Mar.-Apr. 2019. tab, graf
Article in English | LILACS (Americas) | ID: biblio-1001128

ABSTRACT

Abstract This study aims to evaluate tissue healing efficacy in burn patients treated with 1% silver sulfadiazine versus other treatments. This is a systematic literature review and meta-analysis of randomized clinical trials performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyzes (PRISMA) and PICO strategy, registered in the International Prospective Register of Systematic Reviews (PROSPERO) under the number CRD42017081057. The review found 71 studies in MEDLINE/Pubmed, 1 in Clinical Trials, 19 in the Cochrane Library, and 4 in LILACS in five manual searches. Of these, 81 studies were pre-selected. After independent analysis by two reviewers, only 11 studies met the inclusion criteria for the review. All studies (n = 11) using alternative treatments to silver sulfadiazine were shown to be superior in the mean time for complete wound healing, with statistically significant differences between experimental and control groups (p <0.00001); mean difference (- 4.26), 95% CI [- 5.96, - 2.56].


Subject(s)
Humans , Silver Sulfadiazine/therapeutic use , Wound Healing/drug effects , Burns/drug therapy , Anti-Infective Agents, Local/therapeutic use , Silver Sulfadiazine/pharmacology , Time Factors , Randomized Controlled Trials as Topic , Treatment Outcome , Hospitalization , Anti-Infective Agents, Local/pharmacology
4.
Rev. Col. Bras. Cir ; 46(6): e20192322, 2019. tab, graf
Article in Portuguese | LILACS (Americas) | ID: biblio-1057185

ABSTRACT

RESUMO Objetivo: avaliar os efeitos da arginina na cicatrização da parede abdominal de ratos Wistar. Métodos: vinte ratos Wistar foram submetidos à laparotomia e separados em dois grupos (arginina e controle), que receberam tratamento diário por via intraperitoneal com arginina (300mg/kg/dia) e solução tampão fosfato em dose equivalente ao peso, respectivamente, durante cinco dias. No sétimo dia pós-operatório, coletaram-se amostras de sangue e da cicatriz da parede abdominal de ambos os grupos. Avaliaram-se o nível sérico de nitratos e nitritos, a evolução cicatricial pelas dosagens de hidroxiprolina tecidual, formação de tecido de granulação, determinação da porcentagem de colágeno maduro e imaturo, densidade de miofibroblastos e angiogênese. Empregaram-se os testes de ANOVA e t de Student com p=0,05 para as comparações entre os grupos. Resultados: não ocorreram diferenças significantes entre os grupos estudados para dosagens de nitratos e nitritos (p=0,9903), hidroxiprolina tecidual (p=0,1315) e densidade de miofibroblastos (p=0,0511). O grupo arginina apresentou maior densidade microvascular (p=0,0008), maior porcentagem de colágeno tipo I (p=0,0064) e melhora na formação do tecido de granulação, com melhores índices de proliferação angiofibroblástica (p=0,0007) e re-epitelização das bordas (p=0,0074). Conclusão: na avaliação cicatricial da parede abdominal de ratos Wistar sob tratamento com arginina, não houve alteração do nível sérico de nitratos e nitritos, da deposição de colágeno total e da densidade de miofibroblastos. Verificaram-se aumento da maturação de colágeno do tipo I, da densidade microvascular e melhora na formação do tecido de granulação cicatricial pelas melhores re-epitelização de bordas e proliferação angiofibroblástica.


ABSTRACT Objective: to evaluate the effects of arginine on abdominal wall healing in rats. Methods: we submitted 20 Wistar rats to laparotomy and divided them into two groups, arginine and control, which then received, respectively, daily intraperitoneal treatment with arginine (300mg/kg/day) and weight-equivalent phosphate buffered solution, during five days. On the seventh postoperative day, we collected blood and scar wall samples from both groups. We evaluated serum nitrate and nitrite levels, wound evolution by tissue hydroxyproline dosages, granulation tissue formation, percentage of mature and immature collagen, myofibroblast density and angiogenesis. We used the ANOVA and the Student's t tests with p=0.05 for comparisons between groups. Results: there were no significant differences between the groups studied for nitrate and nitrite (p=0.9903), tissue hydroxyproline (p=0.1315) and myofibroblast density (p=0.0511). The arginine group presented higher microvascular density (p=0.0008), higher percentage of type I collagen (p=0.0064) and improved granulation tissue formation, with better angiofibroblastic proliferation rates (p=0.0007) and wound edge reepithelization (p=0.0074). Conclusion: in the abdominal wall healing evaluation of Wistar rats under arginine treatment, there was no change in serum nitrate and nitrite levels, total collagen deposition and myofibroblast density. There was an increase in type I collagen maturation, microvascular density and improvement in scar granulation tissue formation by better edge reepithelization and angiofibroblastic proliferation.


Subject(s)
Animals , Rats , Arginine/pharmacology , Wound Healing/drug effects , Collagen/drug effects , Abdominal Wall/surgery , Collagen/metabolism , Rats, Wistar , Models, Animal , Abdominal Wall/pathology , Myofibroblasts/drug effects , Abdominal Injuries/drug therapy
5.
Rev. Col. Bras. Cir ; 46(5): e20192260, 2019. tab, graf
Article in Portuguese | LILACS (Americas) | ID: biblio-1057172

ABSTRACT

RESUMO Objetivo: avaliar o uso do curativo de filme e gel de biopolímero de celulose bacteriana no tratamento de pacientes com feridas isquêmicas submetidos à revascularização dos membros inferiores. Métodos: ensaio clínico randomizado realizado no ambulatório de Angiologia e Cirurgia Vascular do Hospital das Clínicas da Universidade Federal de Pernambuco, entre janeiro de 2017 e dezembro de 2018. Foram acompanhados 24 pacientes após revascularização de membros inferiores, divididos em dois grupos: Experimental, tratado com filme e gel de biopolímero de celulose bacteriana, e Controle, tratado com ácidos graxos essenciais. Os pacientes foram acompanhados em consultas semanais para troca dos curativos e o processo de cicatrização das feridas foi avaliado em um período de 90 dias. Resultados: a redução da área das feridas isquêmicas no período de 30 dias foi de 4,3cm2 (55%), em média, para o grupo experimental, e de 5,5cm2 (48,5%) para o grupo controle (p>0,05). A taxa de cicatrização completa, em 90 dias, foi de 34,8%, sendo 50% no grupo experimental e 18,2% no grupo controle (p=0,053). Conclusão: o filme de biopolímero de celulose bacteriana associada a gel pode ser utilizado como curativo no tratamento de feridas isquêmicas de pacientes submetidos à revascularização de membros inferiores


ABSTRACT Objective: to evaluate the use of a bacterial cellulose biopolymer film and gel dressing in the treatment of patients with ischemic wounds submitted to lower limb revascularization. Methods: we conducted a randomized clinical trial in the Angiology and Vascular Surgery outpatient clinic of the Clinics Hospital of the Federal University of Pernambuco, between January 2017 and December 2018. We followed 24 patients after lower limb revascularization, divided into two groups: Experimental, treated with bacterial cellulose biopolymer film and gel, and Control, treated with essential fatty acids. Patients attended weekly appointments to change dressings and had their wound healing processes evaluated over a period of 90 days. Results: the reduction of the ischemic wounds' areas after 30 days was 4.3cm2 (55%) on average for the experimental group, and the 5.5cm2 (48.5%) for the control group (p>0.05). The complete healing rate at 90 days was 34.8%, 50% in the experimental group and 18.2% in the control group (p=0.053). Conclusion: the bacterial cellulose biopolymer film associated with gel can be used as a dressing in the treatment of ischemic wounds of patients undergoing revascularization of the lower limbs.


Subject(s)
Humans , Male , Female , Aged , Aged, 80 and over , Bandages , Wound Healing/drug effects , Biopolymers/therapeutic use , Cellulose/therapeutic use , Lower Extremity/pathology , Ischemia/complications , Ischemia/therapy , Time Factors , Treatment Outcome , Angioplasty , Lower Extremity/blood supply , Gels/therapeutic use , Ischemia/pathology , Middle Aged
6.
Rev. Col. Bras. Cir ; 46(5): e20192245, 2019. tab, graf
Article in Portuguese | LILACS (Americas) | ID: biblio-1057168

ABSTRACT

RESUMO Objetivo: comparar a cicatrização, por segunda intenção, sob os efeitos da aplicação tópica de mel, óleo-resina de copaíba e um produto comercial (fibrinolisina, desoxirribonuclease e cloranfenicol) a um grupo controle, em ratos. Métodos: ressecção de pele, com 1cm de diâmetro, foi realizada no dorso de 40 ratos alocados em quatro grupos de dez animais. Todas as feridas foram limpas, diariamente, com 2ml de solução de NaCl 0,9%. O primeiro grupo (controle - GC) ficou restrito a tal procedimento. Nas feridas do segundo (GM), terceiro (GO) e quarto grupos (GF), após limpeza, aplicou-se, respectivamente, 1ml de mel, 1ml de óleo-resina de copaíba e 1ml de creme contendo fibrinolisina, desoxirribonuclease e cloranfenicol. Ocluíram-se as feridas com gaze estéril. Imediatamente após a incisão e nos dias três, sete e 14 do experimento, as feridas foram copiadas e, usando planimetria, analisou-se a contração. Após a eutanásia, a histologia foi utilizada para avaliação da reação inflamatória e do colágeno nas cicatrizes. Resultados: a redução da área da ferida do GM (p=0,003), GO (p=0,011) e GF (p=0,002) foram superiores ao do GC. A quantidade de colágeno tipo I presente no GM e no GO foi superior aos grupos GC e GF (p<0,05). Houve predominância do estágio inflamatório crônico no GM (p=0,004), GO (p<0,001) e GF (p=0,003) quando comparados ao GC. Conclusão: o uso tópico do mel e do óleo-resina de copaíba aumenta a contração da ferida, a presença de colágeno tipo I e acelera a cicatrização.


ABSTRACT Objective: to compare the healing by second intention under the effects of topical application of honey, copaíba oil-resin and a commercial product (fibrinolysin, deoxyribonuclease and chloramphenicol) with a control group in rats. Methods: we carried out a skin resection, 1cm in diameter, on the back of 40 rats allocated to four groups of ten animals. All wounds were cleaned daily with 2ml of 0.9% NaCl solution. The first group (control - GC) was restricted to such procedure. In the wounds of the second (GM), third (GO) and fourth groups (GF), after cleaning, we respectively applied 1ml of honey, 1ml of copaíba oil-resin and 1ml of cream containing fibrinolysin, deoxyribonuclease and chloramphenicol. The wounds were occluded with sterile gauze. Immediately after the incision and on days three, seven and 14 of the experiment, the wounds were copied and contraction was analyzed using planimetry. After euthanasia, we histologically evaluated the inflammatory reaction and collagen in the scars. Results: the reduction of the wound area of GM (p=0.003), GO (p=0.011) and GF (p=0.002) were higher than the GC. The amount of type-I collagen present in GM and GO was higher than in GC and GF groups (p<0.05). There was a predominance of chronic inflammatory stage in GM (p=0.004), GO (p<0.001) and GF (p=0.003) when compared with GC. Conclusion: the topical use of honey and copaíba oil-resin increases wound contraction, the presence of type-I collagen and accelerates healing.


Subject(s)
Animals , Male , Rats , Wound Healing/drug effects , Plant Oils/administration & dosage , Plant Extracts/administration & dosage , Honey , Fabaceae/chemistry , Anti-Infective Agents/administration & dosage , Chloramphenicol/administration & dosage , Administration, Topical , Rats, Wistar , Fibrinolysin/administration & dosage , Deoxyribonuclease I/administration & dosage , Disease Models, Animal
7.
Acta cir. bras ; 34(1): e20190010000004, 2019. tab, graf
Article in English | LILACS (Americas) | ID: biblio-983681

ABSTRACT

Abstract Purpose: To characterize qualitatively and quantitatively the absorption of metronidazole solution, in greater concentrations and for longer periods, when applied topically to an experimental open skin wound model. Methods: An open skin wound, 2 cm in diameter and total skin thickness was prepared, under anesthetic, in the dorsal region of 108 Wistar rats weighing between 300 and 350 grams. The animals were allocated to groups of 18 animals in accordance with the concentration of metronidazole in the solution to be applied daily to the wound. In the control group (CG), 0.9% sodium chloride solution was used for application, and in the experimental groups (GI, GII, GIII, GIV and GV) metronidazole solution at 4%, 6%, 8%, 10% and 12%, respectively, was applied. After 3, 7 and 14 days of treatment. Blood samples collected through cardiac puncture were examined for the existence or non-existence of metronidazole, using high performance liquid chromatography (HPLC). Detected metronidazole values were compared statistically within each group (temporal analysis 3 days X 7 days X 14 days) and between the groups that used topical metronidazole (4% X 6% X 8% X 10% and 12%) using the Kruskal-Wallis test, considering a statistical significance of 95% (p<0.05). Results: Metronidazole was detected in all the samples at all times in all the groups in which topical metronidazole was applied to the wounds. Characteristically, there was no significant difference between the doses obtained within each group over time (3 days X 7 days X 14 days) GI=0.461; GII=0.154; GIII=0.888; GIV= 0.264 and GV=0.152. In the evaluation between groups, a similar degree of absorption was found after 3 days (p=0.829) and 14 days (p=0.751). Conclusion: The serum concentration of metronidazole that was achieved was not influenced by the concentration of the solution applied to the skin wound, with similar extend, or by the duration of the application.


Subject(s)
Animals , Male , Rats , Wound Healing/drug effects , Metronidazole/administration & dosage , Metronidazole/blood , Anti-Infective Agents/administration & dosage , Anti-Infective Agents/blood , Time Factors , Administration, Topical , Chromatography, Liquid , Rats, Wistar , Disease Models, Animal
8.
Acta cir. bras ; 34(3): e201900306, 2019. tab, graf
Article in English | LILACS (Americas) | ID: biblio-989063

ABSTRACT

Abstract Purpose: To investigate the effects of allopurinol administration on osteoinductive reaction and bone development with graft material. Methods: Thirty-six Wistar albino rats were divided into 3 groups. In the control group, calvarial bone defect was only created without any treatment. In the Defect + Graft group, allograft treatment was performed by forming 8 mm calvarial bone defect. In the Defect + Graft + Allopurinol group, alloplastic bone graft was placed in the calvarial bone defect and then, allopurinol (50 mg/kg/day) treatment was intraperitoneally applied for 28 days. Results: Histopathological examination revealed inflammation, congestion in the vessels, and an increase in osteoclast cells in the defect area. We also observed that new osteocyte cells, increase in connective tissue fibers, and new bone trabeculae. Osteopontin expression was positive in osteoblast cells and lacunated osteocyte cells were located in the periphery of the new bone trabeculae. Osteopontin expression was also positive in osteoblasts and osteocytes cells of new bone trabeculae in the graft site. Conclusion: It has been shown that allopurinol treatment in rat calvaria defects may induce osteoblastic activity, matrix development, mature bone cell formation and new bone formation when used with autogenous grafts.


Subject(s)
Animals , Rats , Osteogenesis/drug effects , Skull/drug effects , Wound Healing/drug effects , Bone Regeneration/drug effects , Allopurinol/pharmacology , Skull/injuries , Rats, Wistar , Disease Models, Animal , Autografts
9.
Acta cir. bras ; 34(3): e201900307, 2019. tab, graf
Article in English | LILACS (Americas) | ID: biblio-989071

ABSTRACT

Abstract Purpose: To evaluate the healing potential of the Ximenia americana hydroalcoholic extract in 10% cream in excisional wound models in rats. Methods: Sixty male adults Wistar rats were submitted to skin and subcutaneous tissue surgery in the right and left thoracic regions, divided into three experimental groups: Standard submitted to treatment with only the base vehicle, Treated wounds treated with hydroalcoholic extract of X. americana applied on 10%, Lanette base and Control, untreated wounds. The treatment was performed daily and the wounds evaluated microscopically by the quantification of fibroblasts, collagen fibers and blood vessels. Results: The histomorphometric analysis showed a significant increase in the number of fibroblasts, collagen fibers and blood vessels in the treated group. Conclusion: The topical action of the cream based on Ximenia americana shows angiogenic effects and improves the replacement of collagen, suggesting its use for the development of herbal remedy in the treatment of cutaneous wound healing.


Subject(s)
Animals , Male , Rats , Wound Healing/drug effects , Plant Extracts/therapeutic use , Olacaceae/chemistry , Ointments , Rats, Wistar , Disease Models, Animal
10.
Rev. Col. Bras. Cir ; 46(1): e2015, 2019. tab, graf
Article in Portuguese | LILACS (Americas) | ID: biblio-990367

ABSTRACT

RESUMO Objetivo: avaliar os efeitos da administração tópica do metronidazol na diferenciação de fibroblastos e na contração da ferida durante cicatrização experimental por segunda intenção em ratos. Métodos: cento e oito animais foram submetidos a uma ferida circular no dorso, com 2cm de diâmetro e divididos em seis grupos: grupo controle, com aplicação de solução salina sobre a ferida e cinco grupos experimentais divididos de acordo com a concentração da solução do metronidazol utilizada (4%, 6%, 8%,10% e 12%). Curativos foram realizados diariamente durante todo o período do experimento, que foi subdividido em três momentos de análise: três, sete e 14 dias. A contração da ferida foi avaliada por planimetria digital e os miofibroblastos e protomiofibroblastos foram identificados usando técnicas de imuno-histoquímica CD34 e a-SMA. Resultados: a contração da ferida não apresentou diferença entre os grupos e o controle. Os protomiofibroblastos foram significativamente mais numerosos aos sete dias (p=0,022) nos grupos metronidazol de 4%, 6% e 8%. Após 14 dias, nos mesmos grupos, os miofibroblastos predominaram significativamente (p=0,01). Conclusão: a administração tópica de solução de metronidazol em feridas de pele com cicatrização por segunda intenção foi capaz de melhorar a diferenciação de fibroblastos. A fase de contração da cicatrização de feridas permaneceu inalterada, sem redução significativa da contração avaliada pela planimetria digital. Estes resultados podem ser utilizados em favor do processo de cicatrização de feridas.


ABSTRACT Objective: to assess the effects of topical administration of metronidazole on fibroblast differentiation and on wound contraction during experimental secondary intention wound healing in rats. Methods: we submitted 108 rats to a circular wound on the back, 2cm in diameter, and divided them into six groups: control group, with application of saline solution on the wound and five experimental groups, divided according to the concentration of metronidazole solution used (4%, 6%, 8%, 10% and 12%). We changed the dressings daily throughout the trial period, which comprised three stages of analysis: three, seven and 14 days. We evaluated wound contraction by digital planimetry, and identified myofibroblasts and protomyofibroblasts using CD34 and α-SMA immunohistochemistry techniques. Results: wound contraction was not different between the experimental and the control groups. Protomyofibroblasts were significantly more numerous at seven days (p=0.022) in the 4%, 6% and 8% metronidazole groups. After 14 days, in the same groups, myofibroblasts predominated significantly (p=0.01). Conclusion: the topical administration of metronidazole solution in skin wounds healing by secondary intention was able to improve the differentiation of fibroblasts. The contraction phase of wound healing remained unchanged, without significant reduction of the contraction evaluated by digital planimetry. These results can be used in favor of the wound healing process.


Subject(s)
Animals , Male , Rats , Wound Healing/drug effects , Myofibroblasts/drug effects , Metronidazole/administration & dosage , Anti-Infective Agents/administration & dosage , Administration, Topical , Rats, Wistar , Disease Models, Animal
11.
Acta cir. bras ; 34(12): e201901202, 2019. graf
Article in English | LILACS (Americas) | ID: biblio-1054685

ABSTRACT

Abstract Purpose To explore the potential role and unclear molecular mechanisms of vaccarin in wound healing. Methods Rats' skin excision model to study the effects of vaccarin on wound healing in vivo . Hematoxylin and eosin staining was performed to evaluate Histopathologic characteristics. Immunohistochemistry was employed to assess the effects of vaccarin in accelerating angiogenesis. Western blot was used to evaluate relative protein expressed levels. Results Vaccarin could significantly promote wound healing and endothelial cells and fibroblasts proliferation in the wound site. Immunohistochemistry and Western blot studies showed that the nodal proteins and receptor (bFGFR) related to angiogenesis signaling pathway were activated, and the microvascular density in the wound site was markedly higher than that in the control group. Conclusions The present study was the first to demonstrate that vaccarin is able to induce angiogenesis and accelerate wound healing in vivo by increasing expressions of p-Akt, p-Erk and p-bFGFR. This process is mediated by MAPK/ERK and PI3K/AKT signaling pathways.


Subject(s)
Animals , Male , Wound Healing/drug effects , Plant Extracts/pharmacology , Phosphatidylinositol 3-Kinases/drug effects , Mitogen-Activated Protein Kinase Kinases/drug effects , Caryophyllaceae/chemistry , Angiogenesis Inducing Agents/pharmacology , Time Factors , Immunohistochemistry , Plant Extracts/chemistry , Signal Transduction , Blotting, Western , Reproducibility of Results , Rats, Sprague-Dawley , Phosphatidylinositol 3-Kinases/analysis , Mitogen-Activated Protein Kinase Kinases/analysis , Endothelial Cells/drug effects , Cell Proliferation/drug effects , Receptor, Fibroblast Growth Factor, Type 1/analysis , Receptor, Fibroblast Growth Factor, Type 1/drug effects , Fibroblasts/drug effects
12.
J. coloproctol. (Rio J., Impr.) ; 38(4): 309-313, Oct.-Dec. 2018. tab, graf
Article in English | LILACS (Americas) | ID: biblio-975977

ABSTRACT

ABSTRACT Ulcerative colitis is a chronic inflammatory condition of the colon with an unknown etiology. In this study, we aimed to evaluate the therapeutic effects of Cupressus sempervirens extract on the healing of acetic acid-induced ulcerative colitis in rat. Fifty-five male rats divided into five equal treatment groups were used for this study and received the following treatments: Group 1, 250 mg/kg asacol; Group 2, 1 ml gel base (carboxymethyl cellulose); Group 3, 0.5% gel form of C. sempervirens extract; Group 4, 1% gel form of C. sempervirens extract, and; Group 5, considered as negative control and received 1 ml of normal saline. Body weight changes, histopathological and antioxidant changes in the colon tissue were evaluated. Significant weight gain was observed in rats that received 1% gel extract of C. sempervirens. Significant superoxide dismutase activity was also detected in 0.5 and 1% gel extract groups compared to C. sempervirens extract, Asacol and in 1% gel extract groups compared to the gel base group. Furthermore, both gel extract groups had significant lower total antioxidant capacity compared to Asacol group. Several histopathological lesions including inflammation, ulceration, crypt disarray, and goblet cell depletion were detected in the different groups, however, the mean rank of pathological changes showed no significant difference among the five groups. In summary, our results showed that hydroalcoholic extracts of C. sempervirens leaves produces healing effects in acetic acid induced ulcerative colitis.


RESUMO A colite ulcerativa é uma doença inflamatória crônica do cólon com uma etiologia desconhecida. O objetivo deste estudo foi avaliar os efeitos terapêuticos do extrato de Cupressus sempervirens na cicatrização de colite ulcerativa induzida por ácido acético em ratos. Cinquenta e cinco ratos machos divididos em cinco grupos de tratamento iguais foram utilizados para este estudo e receberam os seguintes tratamentos: Grupo 1: 250 mg/kg de asacol; Grupo 2: 1 mL de gel base (carboximetilcelulose); Grupo 3: extrato de C. sempervirens a 0,5% em gel; Grupo 4: extrato de C. sempervirens a 1% em gel e; Grupo 5: considerado controle negativo que recebeu 1 mL de solução salina normal. Alterações no peso corporal, alterações histopatológicas e antioxidantes no tecido do cólon foram avaliadas. Ganho de peso significativo foi observado em ratos que receberam extrato em gel de C. sempervirens a 1%. Atividade significativa de superóxido dismutase também foi detectada em grupos de extrato em gel de 0,5 e 1% em comparação com o extrato de C. sempervirens, Asacol e em grupos de extrato em gel a 1% em comparação com o grupo base de gel. Além disso, ambos os grupos de extrato em gel apresentaram capacidade antioxidante total significativamente menor em comparação ao grupo Asacol. Várias lesões histopatológicas, incluindo inflamação, ulceração, desarranjo da cripta e depleção de células caliciformes foram detectadas nos diferentes grupos; no entanto, a classificação média de alterações patológicas não apresentou diferença significativa entre os cinco grupos. Em resumo, nossos resultados mostraram que extratos hidroalcoólicos de folhas de C. sempervirens produzem efeitos cicatrizantes em colite ulcerativa induzida por ácido acético.


Subject(s)
Animals , Rats , Colitis, Ulcerative , Cupressus australis/therapeutic use , Colon/pathology , Antioxidants , Wound Healing/drug effects , Plant Extracts
13.
Acta cir. bras ; 33(11): 1000-1015, Nov. 2018. tab, graf
Article in English | LILACS (Americas) | ID: biblio-973480

ABSTRACT

Abstract Purpose: To evaluate the effects and mechanisms of andiroba-based emulsion (ABE) topical treatment on full-thickness cutaneous wounds in rats. Methods: The wounds were harvested on days 3, 7, 15, and 20 post-surgery. Wound contraction rate, quantitative immunohistochemistry [macrophages, myofibroblasts, capillaries, collagens (col) I and III, transforming growth factor β3β (TGFβ3)], and tensile strength were assessed. Results: Treated wounds were smaller, contracted earlier and had increased angiogenesis, fewer CD68+ and M2 macrophages on days 7 and 15, but higher on day 20. Myofibroblasts appeared on days 3 to 7 in untreated wounds and on days 7 to 15 in treated wounds. TGFβ3 levels were higher in the treated wounds, less dense collagen fibers, lower col I/III ratios and a higher tensile strength. Conclusion: These results demonstrate the important anti-inflammatory role of treatment and the associated modulation of macrophages, myofibroblasts, and TGFβ3 levels. Collagen fibers in the treated wounds were more organized and less dense, similar to unwounded skin, which likely contributed to the higher tensile strength.


Subject(s)
Animals , Male , Skin/drug effects , Wound Healing/drug effects , Plant Oils/pharmacology , Meliaceae/chemistry , Transforming Growth Factor beta3/drug effects , Anti-Inflammatory Agents/pharmacology , Skin/pathology , Administration, Cutaneous , Immunohistochemistry , Reproducibility of Results , Treatment Outcome , Rats, Wistar , Collagen Type I/analysis , Collagen Type III/analysis , Emulsions , Extracellular Matrix/drug effects , Transforming Growth Factor beta3/analysis , Myofibroblasts/drug effects
14.
Medisan ; 22(8)set.-oct. 2018.
Article in Spanish | LILACS (Americas) | ID: biblio-976157

ABSTRACT

Se realizó un estudio clínico, prospectivo, intervencionista y comparativo, de 41 pacientes (31 ojos) con diagnóstico de queratitis punteada superficial -seleccionados por muestreo aleatorio simple-, atendidos en el Hospital Infantil Docente Sur Dr Antonio María Béguez César de Santiago de Cuba, desde noviembre del 2016 hasta mayo del 2017, con vistas a demostrar la eficacia del tratamiento tópico con colirio de plaquetas plasmáticas en ellos, para lo cual se conformaron 2 grupos: el A, que recibió tratamiento tópico convencional, y el B con la misma terapia más colirio de plasma rico en plaquetas. Los pacientes tuvieron seguimiento clínico a las dos y cuatro semanas de aplicado el tratamiento. En la mayoría de los pacientes del grupo B el tiempo de cicatrización corneal fue de 7 días y el de curación de 2 semanas; de manera que se demostró la eficacia de este colirio autólogo en la cicatrización corneal, sin riesgo de reacción inmunológica y de bajo costo económico.


A clinical, prospective, interventionist and comparative study of 41 patients (31 eyes) with diagnosis of superficial punctate queratitis -selected by simple random sampling-, assisted in Dr Antonio María Béguez Caesar Southern Teaching Pediatric Hospital in Santiago de Cuba was carried out from November, 2016 to May, 2017, with the objective of demonstrating the effectiveness of the topical treatment of plasmatic platelets in them with eyewash, for which 2 groups were formed: group A with conventional topical treatment, and group B with the same therapy plus eyewash with rich platelets plasm. The patients had clinical follow- up at two and four weeks of having applied the treatment. In most of the patients of group B the time of corneal scaring was of 7 days and that of cure of 2 weeks; so that the effectiveness of this autologous eyewash was demonstrated in the corneal scaring, without risk of immunological reaction and with low economic cost.


Subject(s)
Humans , Male , Female , Infant , Child, Preschool , Child , Adolescent , Platelet-Rich Plasma/metabolism , Corneal Injuries/therapy , Keratitis/therapy , Wound Healing/drug effects
15.
Acta cir. bras ; 33(9): 799-805, Sept. 2018. tab
Article in English | LILACS (Americas) | ID: biblio-973496

ABSTRACT

Abstract Purpose: To evaluate if Moringa oleifera leaf aqueous extract (ME) influences the healing of skin wounds of diabetic rats. Methods: Wistar rats were used (6 rats/group). Group 1 received normal saline (NS) v.o. Group 2 received moringa extract (100mg/kg v.o) for 3 weeks. Groups 3 and 4: Streptozotocin (STZ) induced diabetes. Group 3 received NS; Group 4 received aqueous ME (100mg/kg) v.o.The wounds of groups 1 and 3 rats were topically treated with NS; wounds of groups 2 and 4 treated with 200µL of 10% ME. After anesthesia, all rats had skin square excision wounds 1.5cm2. Wound percent contractions were measured. On 10th day, blood glucose and serum cytokines were measured. Histometry of wounds was studied using ImagePro6.0 software. Results: Glycemia was significantly reduced in ME treated rats. These rats had higher percent contraction of the wounds on 2nd, 5th and 10th days, then controls (p<0.05). Diabetic rats treated with NS had TNF-α, IL-1β and IL-6 expression higher than in rats receiving ME. The histopathological score of ME treated diabetic rats (198±13.7) was significantly higher than treatment with NS (145±10.5). Conclusion: ME extract positively influenced healing of wounds in diabetic rats after systemic and topical treatment.


Subject(s)
Animals , Rats , Wound Healing/drug effects , Plant Extracts/pharmacology , Moringa oleifera/chemistry , Administration, Topical , Interleukin-6/blood , Interleukin-2/blood , Tumor Necrosis Factor-alpha/blood , Rats, Wistar , Streptozocin , Diabetes Mellitus, Experimental
17.
Acta cir. bras ; 33(7): 565-576, July 2018. tab, graf
Article in English | LILACS (Americas) | ID: biblio-949365

ABSTRACT

Abstract Purpose: To investigate the possible effects of argan oil on the healing of colorectal anastomoses. Methods: I n Group 1 (sham), laparotomy was performed and the colon was mobilized. In the control (Group 2) and argan oil (Group 3) groups, colonic resection and anastomosis were applied. To the control and sham groups, 2 mL of 0.9% NaCl was administred rectally, and in the argan oil group, 2 mL/day argan oil was applied rectally for 7 days. Results: The mean bursting pressures of the argan oil and sham groups were significantly higher than the values in the control group. A significant difference was determined between the tissue hydroxyproline and prolidase levels of control group and other groups. Histopathologically, argan oil showed significant beneficial effects on colonic wound healing. In the argan oil and sham groups, the tissue malondialdehyde and fluorescent oxidation product levels were found to be lower and total sulfhydryl levels were higher than the control group. Conclusions: The rectally administered argan oil was observed to have significantly ameliorated wound healing parameters and exerted a significant antioxidant effect. This is the first study in the literature about the beneficial effects of argan oil on colorectal anastomoses.


Subject(s)
Animals , Female , Rectum/surgery , Wound Healing/drug effects , Plant Oils/therapeutic use , Colon/surgery , Anti-Inflammatory Agents/therapeutic use , Antioxidants/therapeutic use , Oxidoreductases/analysis , Rectum/pathology , Spectrophotometry , Anastomosis, Surgical , Random Allocation , Reproducibility of Results , Collagen/analysis , Treatment Outcome , Rats, Wistar , Colon/pathology , Oxidative Stress/drug effects , Dipeptidases/analysis , Surgical Wound/pathology , Surgical Wound/drug therapy , Hydroxyproline/analysis , Malondialdehyde/analysis
18.
Acta cir. bras ; 33(6): 518-523, June 2018. graf
Article in English | LILACS (Americas) | ID: biblio-949357

ABSTRACT

Abstract Purpose: To investigate the therapeutic potential of honey, Nigella sativa (N. sativa) and their combination in rat model of excisional wound healing. Methods: A circular excision wound was established in the back region of 50 Wistar rats. Subsequently, they were divided into 5 groups and daily topical administration of lanolin in the control group, honey in the honey group, cold-pressed N. sativa seed oil in the N. sativa groups, mix of 1:1 ratio of honey and N. sativa seed oil in the mix group, and phenytoin cream in the phenytoin group were used. Then, wound surface areas were evaluated using digital camera immediately after the injury and at post excision days 5, 10, 15 and 20. Results: Significant reduction in wound surface area was observed within and between the groups (P < 0.001). In the post excision days 5, 10, 15 and 20 the wound surface areas in the mix group were significantly lower than the other groups followed by the phenytoin, honey, N. sativa, and control groups. Conclusion: The wound healing may be improved and accelerated by using topical solutions of honey, N. sativa seed oil and especially their mixture.


Subject(s)
Animals , Male , Wound Healing/drug effects , Plant Oils/pharmacology , Nigella sativa/chemistry , Honey , Phenytoin/pharmacology , Time Factors , Administration, Cutaneous , Random Allocation , Reproducibility of Results , Treatment Outcome , Rats, Wistar , Drug Combinations , Drug Synergism , Lanolin/pharmacology
19.
Acta cir. bras ; 33(4): 333-340, Apr. 2018. tab, graf
Article in English | LILACS (Americas) | ID: biblio-886278

ABSTRACT

Abstract Purpose: To compare platelet rich plasma (PRP) and fibrin glue about the effect of anastomotic healing. Methods: Thirty six Wistar-Albino male rats diveded into 3 groups according to control(Group1), PRP (Group 2) and fibrin glue(Tisseel VH) (Group 3). The colon was transected with scissor and subsequently an end to end anastomosis was performed using continuous one layer 6/0 vicryl sutures. Postoperative 7th day effect of anastomotic healing measuring with tissue hydroxyproline(TH) level and anastomotic bursting pressure(ABP); moreover comparison of cytokine (IL-6 and IL-10) and procalcitonin levels on 1st,3rd and 7th days. Results: There was no statistically significant difference of the ABP and hydroxyproline levels between PRP and fibrin glue on the 7th day. There was no statistically significant difference between levels of proinflammatory cytokine (IL-6) (P=0.41), anti-inflammatory cytokine (IL-10) (P=0.35), and procalcitonin levels (P=0.63) on 1, 3 and 7 days. Conclusion: Fibrin glue and platelet rich plasma are shown to be effective in healing intestinal anastomoses without superior to each other.


Subject(s)
Animals , Male , Wound Healing/drug effects , Hemostatics/pharmacology , Fibrin Tissue Adhesive/pharmacology , Platelet-Rich Plasma , Time Factors , Calcitonin/analysis , Anastomosis, Surgical , Reproducibility of Results , Cytokines/analysis , Treatment Outcome , Rats, Wistar , Colon/surgery , Colon/pathology , Hydroxyproline/analysis
20.
Rev. cuba. enferm ; 34(1): e1235, ene.-mar. 2018. tab, graf
Article in Portuguese | LILACS (Americas), CUMED | ID: biblio-1099021

ABSTRACT

RESUMO Introdução: O óleo extraído das sementes da Rosa Mosqueta (Rosa aff rubiginosa ou Rosa canina L.) é rico em ácidos graxos insaturados e ácido transretinóico cujas propriedades têm despertado o interesse desse insumo no processo de cicatrização. Objetivo: Realizar um levantamento de estudos sobre a aplicação tópica do óleo de Rosa Mosqueta para cicatrização de feridas. Métodos: A busca foi realizada em bases de dados eletrônicas entre julho e setembro de 2015 por meio dos descritores "Rosa Mosqueta", "wound", "Rosa aff rubiginosa", "healing" ou "Rosa canina", sendo incluídos artigos originais e de revisão. Foram encontradas 7 publicações, sendo uma revisão de literatura, três estudos experimentais não clínicos, um estudo clínico de delineamento não-experimental, um estudo com delineamento quase-experimental e outro com delineamento experimental, porém de baixa qualidade metodológica. Conclusões: Estudos indicam que em lesões de cicatrização por primeira intenção, o óleo deve ser utilizado após 48 h do procedimento. O óleo refinado é mais estável que o óleo bruto e apresenta efeitos terapêuticos mesmo em baixa concentração. No entanto, os estudos clínicos possuem vieses metodológicos que reduzem o nível de evidência e não são suficientes para uma recomendação. Já os estudos não clínicos mostram que o óleo de Rosa Mosqueta pode ter atividade imunomodulatória no leito da ferida e favorecer a deposição de colágeno e organização tecidual(AU)


RESUMEN Introducción: el aceite extraído de las semillas de Rosa mosqueta (Rosa aff rubiginosa ou Rosa canina L.) presenta en su composición ácidos grasos esenciales y ácido transretinoico, cuyas propiedades han atraído el interés de aplicación de este insumo en el proceso de cicatrización de la piel. Objetivo: llevar a cabo un estudio de encuesta sobre la aplicación tópica de este aceite. Métodos: la investigación se desarrolló en las bases de datos electrónicas entre los meses de julio y septiembre de 2015 com las seguintes palabras clave: "Rosa Mosqueta", "wound", "Rosa aff rubiginosa", "healing" ou "Rosa canina" que incluyó artículos originales y de revisión sin. Fueron obtenidos siete artículos: un artículo de revisión, tres estudios experimentales no clínicos, un estudio clínico de diseño no experimental, un estudio de diseño casi-experimental y otro con el diseño experimental, pero todos ellos con baja calidad metodológica. Conclusiones: los trabajos evaluados indicaron que para la cicatrización de la herida por primera intención, el aceite debe ser utilizado después de 48 horas del procedimiento. El producto refinado es más estable que el crudo y tiene efectos terapéuticos incluso en baja concentración. Sin embargo, los estudios clínicos tienen sesgos metodológicos que reducen el nivel de evidencia, y no son suficientes para una recomendación terapéutica. Los estudios no clínicos mostraron que el aceite de Rosa mosqueta puede tener actividad inmunomoduladora en el lecho de la herida y promover la deposición de colágeno y organización del tejido(AU)


ABSTRACT Introduction: The oil extracted of Rose hip' seeds (Rosa aff rubiginosa or Rosa canina L.) is rich in fatty acids and transretinoic acid whose properties have attracted the interest this input in the healing process. Objective: To conduct a survey of studies on topical application of rosehip oil for wound healing. Methods: The search was conducted on electronic databases between july and September 2015 using the following descriptors: "Rosa Mosqueta", "wound", "Rosa aff rubiginosa", "healing" ou "Rosa canina", which included original and review articles. We found 7 publications: one literature review, three non-clinical experimental studies, one clinical study of non-experimental design, one study of quasi-experimental design and the other with experimental design, but low methodological quality. Conclusions: The studies show that in healing by first intention, the oil must be used 48h after the procedure. The refined oil is more stable than the crude oil and maintain therapeutic effects even at lower concentrations. However, clinical studies contain methodological bias to reduce the level of evidence and are not sufficient for appropriate recommendation. The non-clinical studies show that rosehip oil may have immunomodulatory activity in wound bed and promotes collagen deposition and tissue organization(AU)


Subject(s)
Humans , Wound Healing/drug effects , Clinical Trials as Topic/methods , Rosa/adverse effects , Databases as Topic
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