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1.
Rev. bras. med. esporte ; 27(3): 295-298, July-Sept. 2021. graf
Article in English | LILACS | ID: biblio-1288566

ABSTRACT

ABSTRACT Introduction Skeletal muscle injuries account for 10% to 50% of treadmill sports injuries. Insulin-like growth factor (IGF) is a family of polypeptides with both insulin-like anabolic and growth-promoting effects. Sports play a vital role in the recovery of skeletal muscle injuries. Objective The paper analyzes the ability of insulin-like growth factor 1 (IGF-1) to repair skeletal muscle injury caused by treadmill exercise. Method We injected drugs under the wound after exercise-induced injury in rats. The control group was injected with saline, and the experimental group was injected with an insulin-like growth factor. We conduct histological and electron microscopic structural analysis of rats, Results: After an injury, the experimental group formed a basal lamina protective film earlier than the control group, activated myoblasts, formed myofilaments, formed myotubes, and fused into muscle fibers earlier than the control group. The healing quality was also better. The experimental group was endogenous. The mRNA content of sex IGF-1 and IGF-2 both increased earlier than the control group. Conclusion Local injection of exogenous insulin-like growth factor-1 can stimulate the proliferation of myoblasts and accelerate the post-traumatic repair process of skeletal muscle caused by treadmill sports. Level of evidence II; Therapeutic studies - investigation of treatment results.


RESUMO Introdução As lesões do músculo esquelético representam de 10% a 50% das lesões em esteira esportiva. O fator de crescimento semelhante à insulina (IGF) é uma família de polipeptídeos com efeitos anabólicos e de promoção do crescimento semelhantes à insulina. Os esportes desempenham um papel vital na recuperação de lesões musculares esqueléticas. Objetivo o artigo analisa a capacidade do fator de crescimento semelhante à insulina 1 (IGF-1) em reparar lesões musculares esqueléticas causadas por exercícios em esteira. Método Injetamos drogas sob a ferida após lesão induzida por exercício em ratos. O grupo controle foi injetado com solução salina e o grupo experimental foi injetado com um fator de crescimento semelhante à insulina. Realizamos análises histológicas e microscópicas eletrônicas estruturais de ratos. Resultados Após a lesão, o grupo experimental formou um filme protetor da lâmina basal mais cedo do que o grupo controle, mioblastos ativados, miofilamentos formados, miotubos formados e fundidos em fibras musculares mais cedo do que o grupo controle. A qualidade da cura também foi melhor. O grupo experimental era endógeno. O conteúdo do sexo IGF-1 e IGF-2 mRNA aumentou mais cedo do que no grupo de controle. Conclusão A injeção local de fator de crescimento semelhante à insulina 1 exógeno pode estimular a proliferação de mioblastos e acelerar o processo de reparo muscular esquelético pós-traumático causado por esportes em esteira. Nível de evidência II; Estudos terapêuticos: investigação dos resultados do tratamento.


RESUMEN Introducción Las lesiones del músculo esquelético representan del 10% al 50% de las lesiones deportivas en cinta. El factor de crecimiento semejante a la insulina (IGF) es una familia de polipéptidos con efectos anabólicos y estimulantes del crecimiento semejantes a la insulina. Los deportes juegan un papel vital en la recuperación de las lesiones del músculo esquelético. Objetivo El artículo analiza la capacidad del factor de crecimiento semejante a la insulina 1 (IGF-1) para reparar la lesión del músculo esquelético causada por el ejercicio en cinta. Método inyectamos drogas debajo de la herida después de una lesión inducida por el ejercicio en ratas. Al grupo de control se le inyectó solución salina y al grupo experimental se le inyectó un factor de crecimiento semejante a la insulina. Realizamos análisis estructurales histológicos y microscópicos electrónicos de ratas, Resultados: Después de una lesión, el grupo experimental formó una película protectora de la lámina basal antes que el grupo de control, activó mioblastos, formó miofilamentos, formó miotubos y se fusionó en fibras musculares antes que el grupo de control. La calidad de curación también fue mejor. El grupo experimental fue endógeno. El contenido de ARNm de IGF-1 e IGF-2 de sexo aumentaron antes que en el grupo de control. Conclusión La inyección local de factor de crecimiento semejante a la insulina 1 exógeno puede estimular la proliferación de mioblastos y acelerar el proceso de reparación postraumático del músculo esquelético causado por los deportes en cinta. Nivel de evidencia II; Estudios terapéuticos: investigación de los resultados del tratamiento.


Subject(s)
Humans , Male , Rats , Wound Healing/drug effects , Insulin-Like Growth Factor I/administration & dosage , Muscle, Skeletal/injuries , Acute Disease , Muscle, Skeletal/drug effects , Muscle, Skeletal/ultrastructure , Disease Models, Animal
2.
Rev. bras. oftalmol ; 80(1): 17-20, jan.-fev. 2021. tab
Article in English | LILACS | ID: biblio-1251318

ABSTRACT

ABSTRACT Objective: To observe clinically, in rabbits, the side effects of topical injection of subconjunctival cyclophosphamide, studying its role as an antifibrotic drug. Methods: Prospective study in 20 albino rabbits of New Zealand race. All rabbits were treated with cyclophosphamide, 10mg/ml in a volume of 0.3 ml, in the left eye through subconjunctival injection. They were evaluated for 1, 7, 30, and 60 days after the procedure. All the animals were examined for the detection of ocular reactions such as necrosis, hyperemia, chemosis, secretion, opacity, and iritis. Other side effects as changes in the behavior, in the feed, and the water consumption were also evaluated. Results: It was observed that from the 20 rabbits studied, three rabbits (15%) showed side effects only at the 24 hours analysis. One rabbit (5%) presented hyperemia, one rabbit (5%) had hyperemia associated with iritis, and one rabbit (5%) presented hyperemia associated with secretion. These reactions were not observed at 1, 7, 30, and 60 days. Conclusion: Cyclophosphamide subconjunctival injection induces minor side effects on the conjunctiva of rabbits such as hyperemia, associated with iritis and secretion.


RESUMO Objetivo: Observar clinicamente os efeitos colaterais de injeção subconjuntival de ciclofosfamida, pensando em sua ação como um agente antifibrótico. Métodos: Estudo prospectivo realizado com 20 coelhos albinos da raça Nova Zelândia. Todos os coelhos foram submetidos a 0,3 ml de injeção subconjuntival de ciclofosfamida 10mg/ml no olho esquerdo e foram avaliados de acordo com os efeitos locais no primeiro dia após a injeção, 7, 30 e 60 dias. Foram examinados para detecção de reações oculares como necrose, hiperemia, quemose, secreção, opacidade corneana, irite além de alterações comportamentais e variação no consumo de água e alimentação. Resultados: Dos 20 coelhos estudados, apenas 3 apresentaram reações oculares e somente na leitura de 24 horas. Um coelho (5%) apresentou hiperemia, 1 coelho (5%) apresentou hiperemia associada a presença de irite e 1 coelho (5%) apresentou hiperemia associada a presença de secreção. As reações não foram mais observadas durante os exames de 7, 30 e 60 dias. Conclusão: A ciclofosfamida subconjuntival causou poucos efeitos colaterais na conjuntiva dos coelhos. Os únicos efeitos encontrados foram hiperemia, irite e secreção.


Subject(s)
Animals , Rabbits , Fibrosis/prevention & control , Conjunctiva/drug effects , Cyclophosphamide/adverse effects , Cyclophosphamide/pharmacology , Wound Healing/drug effects , Prospective Studies , Mitomycin/pharmacology , Cyclophosphamide/administration & dosage , Injections, Intraocular , Fibroblasts/drug effects , Fibroblasts/metabolism , Slit Lamp Microscopy
3.
An. bras. dermatol ; 95(4): 418-427, July-Aug. 2020. graf
Article in English | ColecionaSUS, LILACS, ColecionaSUS | ID: biblio-1130916

ABSTRACT

Abstract Background Dermabrasion is related with mechanical and surgical traumas on the skin; usually topical antiseptics and/or saline have been used for healing. Natural products for wound healing can also be used for abrasions, such as latex from Hevea brasiliensis. Objective This study aimed to evaluate the in vitro viability and migratory/proliferative effects of latex serum from H. brasiliensis and to compare with a commercially available standard antiseptic solution and saline in experimental dermabrasion on rats. Methods For in vitro evaluation, MTT and scratch assays were used. In vivo testing was performed in 72 rats submitted to dermabrasion, treated with saline, antiseptic, or latex serum. This study evaluated re-epithelialization, neutrophilic infiltration, and the quantification of crust and epidermis. Results Latex showed viability at 1% and 0.1% concentrations and migratory/proliferative activity at 0.01% concentrations. The re-epithelialization was highest in latex group on 7th day. The latex group displayed lower thickness of crusts and greater extent of epidermal layers. The latex and antiseptic groups showed increases of myeloperoxidase levels on the 2nd day and showed important reductions from the 7th day. Study limitations Acute superficial wound model in rats and non-use of gel-cream (medium) without latex. Conclusion In conclusion, non-toxic latex stimulated migration/proliferation of keratinocytes in vitro and significantly accelerated wound healing in animal excoriation models compared to chlorhexidine or saline.


Subject(s)
Animals , Male , Rats , Wound Healing/drug effects , Plant Extracts/pharmacology , Hevea , Latex/pharmacology , Rats, Wistar , Dermabrasion
4.
Arq. bras. med. vet. zootec. (Online) ; 72(4): 1286-1294, July-Aug. 2020. tab, ilus
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1131465

ABSTRACT

Cicatrização de ferida é um processo dinâmico, que tem por objetivo restaurar a continuidade do tecido lesionado. No entanto, em alguns casos, é necessário favorecer condições adequadas para viabilizar o processo fisiológico. Neste estudo foram utilizados ratos Wistar, divididos aleatoriamente entre cinco grupos, com 12 animais cada, sendo eles: grupo P (Bidens pilosa L.), grupo mel, grupo Co1 (pomada comercial alopática), grupo Co2 (pomada comercial homeopática) e grupo CT (controle). As lesões foram geradas por incisão com punch de 8mm, sendo tratadas diariamente de forma tópica. Foram eutanasiados quatro animais por grupo, no terceiro, sétimo e 14º dias do experimento, e o material coletado foi armazenado em formalina 10% e encaminhado para processamento histológico. Posteriormente, realizou-se a contagem de leucócitos mononucleares, fibroblastos e neovasos e avaliou-se a arquitetura de fibras colágenas. Os resultados da contagem foram analisados pela ANOVA, seguida pelo teste de Tukey (P<0,05). O modelo experimental proposto neste estudo demonstrou que todos os tratamentos apresentaram potencial cicatrizante, com exceção do mel. A aplicação tópica do creme do extrato de Bidens pilosa L. a 10% apresentou melhor perfil anti-inflamatório; a pomada alopática apresentou boa aderência à superfície da lesão e a pomada homeopática, grande potencial angiogênico, com menor tempo de cicatrização.(AU)


Wound healing is a dynamic process that aims to restore the continuity of injured tissue. However, in some cases it is necessary to favor adequate conditions to enable the physiological process. Wistar rats were randomly divided into 5 groups with 12 animals each, namely: group P (Bidens pilosa L.), group honey, group Co1 (commercial allopathic ointment), group Co2 (commercial homeopathic ointment) and group CT (control). The lesions were generated by an 8mm punch incision and were treated topically daily. Four animals per group were euthanized on the 3rd, 7th and 14th day of the experiment and the collected material was stored in 10% formalin and sent for histological processing, after which mononuclear, fibroblasts and neovascular leukocytes were counted and collagen fiber architecture was evaluated. Counting results were analyzed by ANOVA, followed by Tukey test (p <0.05). The experimental model proposed in this study showed that all treatments had healing potential, except honey. The topical application of 10% Bidens pilosa L. extract cream showed the best anti-inflammatory profile; Allopathic ointment showed good adhesion to the surface of the lesion and homeopathic ointment showed great angiogenic potential with shorter healing time.(AU)


Subject(s)
Animals , Rats , Ointments/therapeutic use , Skin/injuries , Bidens/chemistry , Honey , Wound Healing/drug effects , Wounds and Injuries/therapy , Homeopathic Remedy , Collagen , Rats, Wistar/physiology , Phytotherapeutic Drugs , Fibroblasts
5.
Bol. latinoam. Caribe plantas med. aromát ; 19(3): 314-320, mayo 2020. ilus
Article in English | LILACS | ID: biblio-1116301

ABSTRACT

In order to evaluate the potential of this formulation (P. lentiscus L. oil-based ointment) to heal wounds, experimental wounds were done on guinea pigs and efficiency was comparatively assessed against a reference ointment, Cicaderma®. Wound contraction was performed on days 5, 10 and 15. Tissue sections were also evaluated histopathological on days 7, 14 and 21. Results showed that for all days (5, 10 and 15), the highest wound contraction values were attained for the P. lentiscus oil-based ointment treated group with wound contraction values of 19.38, 55.8 and 77.11%, respectively, as compared to the reference drug Cicaderma® where contractions were 7.97%, 49.53% and 71.44%, respectively. Vehicle and negative control groups however showed no statistically significant wound healing activity on the excision wound model. These experimental studies revealed that the P. lentiscus oil-based ointment displays remarkable wound healing activity, in accordance with its use in traditional medicine.


Con el fin de evaluar el potencial de esta formulación (ungüento a base de aceite de P. lentiscus L.) para curar heridas, se realizaron heridas experimentales en cobayos y se evaluó comparativamente su eficacia respecto de un ungüento de referencia, Cicaderma®. La contracción de la herida se realizó los días 5, 10 y 15. Las secciones de tejido también se evaluaron histopatológicamente los días 7, 14 y 21. Los resultados mostraron que para todos los días (5, 10 y 15), se obtuvieron los valores más altos de contracción de la herida para el grupo tratado con ungüento a base de aceite de P. lentiscus con valores de contracción de la herida de 19.38, 55.8 y 77.11%, respectivamente, en comparación con el medicamento de referencia Cicaderma® en donde las contracciones fueron 7.97%, 49.53% y 71.44%, respectivamente. Sin embargo, los grupos de control de vehículo y negativo no mostraron actividad de curación de heridas estadísticamente significativa en el modelo de herida por escisión. Estos estudios experimentales revelaron que la pomada a base de aceite de P. lentiscus muestra una notable actividad de curación de heridas, de acuerdo con su uso en la medicina tradicional.


Subject(s)
Animals , Male , Guinea Pigs , Ointments/pharmacology , Wound Healing/drug effects , Plant Oils/pharmacology , Pistacia/chemistry , Seeds
6.
Int. j. morphol ; 38(2): 492-498, abr. 2020. graf
Article in Spanish | LILACS | ID: biblio-1056467

ABSTRACT

Para promover la reparación y regeneración muscular, se han desarrollado diferentes estrategias, sin embargo, aún no existe un tratamiento único aceptado. Investigaciones relacionan los efectos terapéuticos de la miel en el proceso de cicatrización de heridas de diversas etiologías. Considerando lo anterior, el propósito de este estudio fue evaluar los efectos de una formulación basada en miel nativa (Ulmoplus®) en la reparación de lesiones musculares por incisión quirúrgica. A cinco conejos (Oryctolagus cuniculus) se les realizó una incisión de 20 mm de largo y 3 mm de ancho en la región anterior de cada pierna, atravesando la epidermis, dermis y el músculo tibial anterior, hasta alcanzar el periostio de la tibia. La pierna izquierda de cada animal fue clasificada como grupo sutura (S) y la derecha como grupo sutura más Ulmoplus® (S+U). Posteriormente, al grupo S+U se le aplicó Ulmoplus® en toda la extensión de la herida. Luego, en ambos grupos, la incisión fue suturada con cuatro puntos intradérmicos (vicryl 2/0). Un animal fue considerado como control para obtener imágenes histológicas del músculo tibial anterior sin injuria. A los 10 días post incisión, fueron tomadas las biopsias del músculo y posteriormente fueron procesadas para su observación en microscopio de luz. El estudio se llevó a cabo considerando las recomendaciones de la Guía para el Cuidado y Uso de Animales de Laboratorio. A los 10 días post incisión, las injurias, en ambos grupos de animales, se encontraban cerradas y sin signos de infección. El músculo tibial anterior de los especímenes del grupo S se encontraba en la fase de reparación, con procesos concomitantes de fagocitosis del tejido necrotizado, regeneración de miofibras y formación de tejido conectivo cicatrizal. El músculo tibial anterior de los animales del grupo S+U se encontraba en la fase de remodelación, ya que la arquitectura muscular se evidenciaba restablecida. Ulmoplus®, como coadyuvante en el proceso de cierre de heridas quirúrgicas de músculo esquelético utilizando sutura convencional, es una buena alternativa, ya que acelera el proceso de reparación muscular, y reduce el tejido conectivo cicatrizal.


To promote muscle repair and regeneration, different strategies have been developed, however, there is still no single accepted treatment. Research relates the therapeutic effects of honey in the wound healing process of various etiologies. The aim of this study was to evaluate the effects of native honey formulation (Ulmoplus®) in the repair of muscle injuries by surgical incision. In five rabbits (Oryctolagus cuniculus) were made an incision 20 mm long and 3 mm wide in the anterior region of each leg, crossing the epidermis, dermis and anterior tibial muscle, until reaching the tibia periosteum. The left leg of each animal was classified as a suture group (S) and the right leg as a suture group plus Ulmoplus® (S + U). Subsequently, Ulmoplus® was applied to the entire S + U group throughout the entire length of the wound. Then, in both groups, the incision was sutured with four intradermal points (vicryl 2/0). An animal was considered as a control to obtain histological images of the anterior tibial muscle without injury. At 10 days post incision, muscle biopsies were taken and subsequently processed for observation under light microscopy. The study was carried out considering the recommendations of the Guide for the Care and Use of Laboratory Animals. At 10 days post incision, the injuries, in both groups of animals, were closed and without signs of infection. The anterior tibial muscle of the S group specimens was in the repair phase, with concomitant processes of phagocytosis of the necrotized tissue, myofiber regeneration and formation of scar connective tissue. The anterior tibial muscle of the animals of the S + U group was in the remodeling phase, since the muscular architecture was evidenced restored. Ulmoplus®, as an adjunct in the process of closing surgical wounds of skeletal muscle using conventional suture, is a good alternative, since it accelerates the muscle repair process and reduces scar connective tissue.


Subject(s)
Animals , Rabbits , Wound Healing/drug effects , Surgical Wound/drug therapy , Honey , Muscles/injuries , Sutures , Disease Models, Animal
7.
Braz. j. med. biol. res ; 53(1): e8621, Jan. 2020. tab, graf
Article in English | LILACS | ID: biblio-1055482

ABSTRACT

The use of specially designed wound dressings could be an important alternative to facilitate the healing process of wounds in the hyperglycemic state. Biocompatible dressings combining chitosan and alginate can speed up wound healing by modulating the inflammatory phase, stimulating fibroblast proliferation, and aiding in remodeling phases. However, this biomaterial has not yet been explored in chronic and acute lesions of diabetic patients. The aim of this study was to evaluate the effect of topical treatment with a chitosan-alginate membrane on acute skin wounds of hyperglycemic mice. Diabetes mellitus was induced by streptozotocin (60 mg · kg-1 · day-1 for 5 days, intraperitoneally) and the cutaneous wound was performed by removing the epidermis using a surgical punch. The results showed that after 10 days of treatment the chitosan and alginate membrane (CAM) group exhibited better organization of collagen fibers. High concentrations of interleukin (IL)-1α, IL-1β, granulocyte colony-stimulating factor (G-CSF), and tumor necrosis factor-alpha (TNF-α) were detected in the first and second days of treatment. G-CSF and TNF-α level decreased after 5 days, as well as the concentrations of TNF-α and IL-10 compared with the control group (CG). In this study, the inflammatory phase of cutaneous lesions of hyperglycemic mice was modulated by the use of CAM, mostly regarding the cytokines IL-1α, IL-1β, TNF-α, G-CSF, and IL-10, resulting in better collagen III deposition. However, further studies are needed to better understand the healing stages associated with CAM use.


Subject(s)
Animals , Male , Rabbits , Bandages , Wound Healing/drug effects , Chitosan/administration & dosage , Cell Proliferation/drug effects , Diabetes Mellitus, Experimental/physiopathology , Alginates/administration & dosage , Time Factors , Biocompatible Materials/administration & dosage , Biomarkers/blood , Collagen/drug effects , Inflammation/prevention & control , Mice, Inbred C57BL
8.
São José dos Campos; s.n; 2020. 46 p. il., graf., tab..
Thesis in Portuguese | LILACS, BBO | ID: biblio-1150852

ABSTRACT

A estimulação elétrica (EE) tem sido usada no tratamento de lesões crônicas apresentando resultados favoráveis no fechamento da lesão cutânea entre outras aplicações clínicas. No entanto, a literatura carece de estudos avaliando se os efeitos da EE no fechamento da lesão oral. Diante disso, torna-se relevante investigar os efeitos da EE durante o processo de reparo da lesão oral em camundongos (Swiss). Métodos: Noventa animais foram divididos em grupos: Controle (C; n=45) que receberam a aplicação da EE com o aparelho desligado e Teste (EE; n=45) que receberam a EE (100 µA; 9 kHz; 660 mVpp; 1x/dia por 3 dias). Uma lesão de espessura total foi realizada com punch de biópsia de 1,5 mm de diâmetro. Os animais foram eutanasiados no 1o, 3o e 5o dia após a confecção da lesão. As amostras foram fixadas em paraformaldeído 4%, descalcificadas em ácido fórmico 20% e emblocadas em parafina. Os parâmetros avaliados foram: fechamento clínico da lesão; distância de reparação epitelial (DRE) e conjuntiva (DRC), concentração de citocinas IL-1ß, IL-6, IL-8, IL-10, TNF-α e VEGF; porcentagem de fibras colágenas e quantificação das proteínas de sinalização Smad2. Resultados: A área de fechamento clínico foi reduzida no grupo EE no 5º dia de avaliação (p=0,01). As DRE e DRC do grupo EE apresentaram menor distância em todos os tempos avaliados (p<0,05) com reduzida concentração de IL-6, TNF-α, IL-10 e VEGF (p<0,05). Não houve diferença na porcentagem de fibras colágenas e na phospho-Smad2. Conclusão: A EE teve efeito positivo nos parâmetros clínicos e histológicos com modulação da resposta inflamatória no início do processo de reparo da lesão(AU)


Background: Since Electrical Stimulation (ES) has used for chronic wounds treatment and other clinical applications showing favorable results in wound closure, it was hypothesized whether it could bring good results in oral wound closure. The aim was to investigate the ES effects during the oral wound healing process in mice (Swiss). Methods: Ninety animals were divided in groups: Control (C; n=45) which received a fictitious ES application, i.e. the ES applicance was shutted off and ES (ES; n=45) which received ES (100 µA; 9 kHz; 660 mVpp) once time a day for 3 days consecutively. A full thickness wound was done using a 1.5 mm diameter biopsy punch in the hard palate. The animals were euthanized on 1st, 3rd and 5th day post-wounding. The samples were fixed, decalcified and embedded in paraffin. The parameters evaluated were: clinical wound closure; distance of epithelial and connective wound edges (EWD and CWD); cytokines (IL-1ß, IL-6, IL-8, IL-10), TNF-α and VEGF by multiplex, percentage of collagen fibers and Smad proteins. Results: Clinical wound closure area was reduced on day 5 in ES group (p = 0.01). Both EWD and CWD distance were shorter in all times (p < 0.05) with a reduction of inflammatory cytokines production (p < 0.05). There were no difference in collagen percentage and phospho-Smad2. Conclusion: ES had a positive effect on clinical and histomorphometric wound closure outcomes showing a modulation of inflammatory response in the beginning of wound healing(AU)


Subject(s)
Mouth Mucosa/injuries , Wound Healing/drug effects , Electric Stimulation/methods , Re-Epithelialization/drug effects , Surgical Wound/complications
9.
J. appl. oral sci ; 28: e20190690, 2020. graf
Article in English | LILACS, BBO | ID: biblio-1101255

ABSTRACT

Abstract Objective To investigate the effects of intro-oral injection of parathyroid hormone (PTH) on tooth extraction wound healing in hyperglycemic rats. Methodology 60 male Sprague-Dawley rats were randomly divided into the normal group (n=30) and DM group (n=30). Type 1 diabetes mellitus (DM) was induced by streptozotocin. After extracting the left first molar of all rats, each group was further divided into 3 subgroups (n=10 per subgroup), receiving the administration of intermittent PTH, continuous PTH and saline (control), respectively. The intermittent-PTH group received intra-oral injection of PTH three times per week for two weeks. A thermosensitive controlled-release hydrogel was synthesized for continuous-PTH administration. The serum chemistry was determined to evaluate the systemic condition. All animals were sacrificed after 14 days. Micro-computed tomography (Micro-CT) and histological analyses were used to evaluate the healing of extraction sockets. Results The level of serum glucose in the DM groups was significantly higher than that in the non-DM groups (p<0.05); the level of serum calcium was similar in all groups (p>0.05). Micro-CT analysis showed that the DM group had a significantly lower alveolar bone trabecular number (Tb.N) and higher trabecular separation (Tb.Sp) than the normal group (p<0.05). The histological analyses showed that no significant difference in the amount of new bone (hard tissue) formation was found between the PTH and non-PTH groups (p>0.05). Conclusions Bone formation in the extraction socket of the type 1 diabetic rats was reduced. PTH did not improve the healing of hard and soft tissues. The different PTH administration regimes (continuous vs. intermittent) had similar effect on tissue healing. These results demonstrated that the metabolic characteristics of the hyperglycemic rats produced a condition that was unable to respond to PTH treatment.


Subject(s)
Animals , Male , Rats , Parathyroid Hormone/pharmacology , Tooth Extraction/methods , Wound Healing/drug effects , Tooth Socket/drug effects , Diabetes Mellitus, Experimental/physiopathology , Osteogenesis/radiation effects , Osteogenesis/physiology , Blood Glucose/analysis , Random Allocation , Calcium/blood , Rats, Sprague-Dawley , Hydrogels , Surgical Wound/drug therapy
10.
Braz. J. Pharm. Sci. (Online) ; 56: e18784, 2020. tab, graf
Article in English | LILACS | ID: biblio-1249176

ABSTRACT

Antibacterial activity and good mechanical properties are some of the characteristics required for an appropriate film dressing. A novel polymer blend was developed for wound healing application. Twenty-four formulations using the polymers chitosan, poly(vinyl alcohol) and/or ɛ-Polylysine and the plasticizer glycerol were designed using factorial design and then the films were prepared by the casting/solvent evaporation method. Seventeen films were obtained among the twenty-four proposed formulations that were characterized by Field Emission Scanning Electron Microscopy (FE-SEM) and Fourier Transform Infrared Spectroscopy (FTIR). Mechanical properties, such as tensile strength (σ), elongation at break (ɛ) and Young's modulus (Y) as well as antibacterial properties were determined. The best candidate was then further analyzed with regard to porosity, Water Vapor Transmission Rate (WVTR), swelling and cytotoxicity experiments. The results showed a film with semi-occlusive characteristics, good mechanical properties and no toxic. Incorporation of ɛ-Polylysine increased antibacterial activity against gram-negative (Escherichia coli) and gram-positive (Staphylococcus aureus) bacteria


Subject(s)
Bandages , Chitosan/pharmacology , Polylysine/pharmacology , Wound Healing/drug effects , Microscopy, Electron, Scanning/methods , Spectroscopy, Fourier Transform Infrared , Glycerol/pharmacology
11.
Int. j. morphol ; 37(3): 1073-1078, Sept. 2019. graf
Article in Spanish | LILACS | ID: biblio-1012398

ABSTRACT

Considerando los avances en las investigaciones que relacionan los efectos terapéuticos de la miel en el proceso de cicatrización de heridas, la presente investigación tuvo como objetivo evaluar y comparar cualitativamente, a través de una análisis morfológico, la respuesta tisular local entre sutura convencional coadyuvada con Ulmoplus® y sin Ulmoplus® en el cierre de la incisión de piel de conejo. Se utilizaron 10 conejos adultos, divididos en grupo S (sutura) y grupo S+U (sutura más Ulmoplus®). Se les realizó una incisión de 10 mm de largo, en la región anterior de la pierna, a través de la epidermis y dermis, hasta llegar a la fascia profunda que cubría al músculo tibial anterior. Posteriormente, solo al grupo S+U se le aplicó Ulmoplus®. Luego, en ambos grupos, la incisión fue suturada con dos puntos intradérmicos de ácido poliglicólico (vicryl 2/0). Después de 10 días post incisión fueron tomadas las biopsias de la piel y procesadas para su observación en microscopio de luz. El estudio se llevó a cabo considerando las recomendaciones de la Guía para el Cuidado y Uso de Animales de Laboratorio. Ambos grupos presentaron una incisión cerrada al día 10, con características microscópicas propias de una fase proliferativa. No obstante, el grupo S+U mostró una cicatriz de menor longitud y de aspecto más plano, cuya epidermis estaba mejor organizada y era de menor grosor, con células basales diferenciadas. A nivel dérmico se observó contracción de la herida, evidenciándose una zona cicatrizal de menor tamaño. Ulmoplus®, es una buena alternativa como coadyuvante en el proceso de cierre de heridas quirúrgicas utilizando sutura convencional, ya que acelera el proceso de cicatrización y permite mejores resultados estéticos de la cicatriz.


Considering the advances in research that relate honey therapeutic effects in wound healing process, the present investigation aimed to evaluate and compare qualitatively, through morphological analysis, the local tissue response between conventional suture with Ulmoplus ® and without Ulmoplus ® in the closure of the rabbit skin incision. We used 10 adult rabbits, divided into group S (suture) and group S + U (suture plus Ulmoplus®). An 10 mm long incision was made in the anterior region of the leg, passing through epidermis and dermis, until reaching the deep fascia that covered the anterior tibial muscle. Subsequently, only the S + U group was given Ulmoplus®. Then, in both groups, the incision was sutured with two intradermal points of polyglycolic acid (vicryl 2/0). After 10 days post incision, biopsies of the skin were taken and processed for observation in a light microscope. The study was carried out considering the recommendations of the Guide for the Care and Use of Laboratory Animals. Both groups had a closed incision at day 10 with microscopic characteristics of a proliferative phase. However, the S + U group showed a scar of shorter length and flatter appearance, whose epidermis was more organized and thinner, with differentiated basal cells. At the dermal level, wound contraction was observed, evidencing a smaller scar area. Ulmoplus®, as a coadjuvant in closing surgical wounds process using conventional suture is a good alternative, since it accelerates the healing process and allows better aesthetic results of the scar.


Subject(s)
Animals , Rabbits , Skin/drug effects , Wound Healing/drug effects , Suture Techniques , Honey , Skin/ultrastructure , Wound Closure Techniques , Dermatologic Surgical Procedures , Surgical Wound
12.
J. oral res. (Impresa) ; 8(supl.1): 15-18, ago. 9, 2019. ilus
Article in English | LILACS | ID: biblio-1141330

ABSTRACT

Objective: To evaluate the effects of luteolin nanoparticles on the process tooth socket healing in rabbits. Design: This study comprised five rabbits randomly assigned to control animal and experimental animals. Immediately after the extraction of an upper maxillary incisor, the alveolar sockets of experimental animals were treated with topical luteolin while alveolar sockets of the control group remained without treatment. The animals were sacrificed by decapitation with deep anesthesia seven days post tooth extraction. The tooth sockets were sectioned and stained with hematoxylin and eosin stains. Results: Histological evaluation revealed that luteolin treatment induced earlier healing of extracted tooth sockets. Conclusion: These findings suggest that luteolin accelerates the healing process in tooth sockets of rabbits.


Subject(s)
Animals , Rabbits , Wound Healing/drug effects , Tooth Socket/drug effects , Luteolin/administration & dosage , Mouth/drug effects , Tooth Extraction , Nanoparticles
13.
Braz. j. otorhinolaryngol. (Impr.) ; 85(4): 473-480, July-Aug. 2019. tab, graf
Article in English | LILACS | ID: biblio-1019583

ABSTRACT

Abstract Introduction: Endoscopic sinus surgery can lead to crusting or synechiae formation, which can affect the healing process. Objective: The aim of our study was to compare the influence of steroid versus antibiotic versus saline solution impregnated absorbable nasal spacers on postoperative wound healing and patient satisfaction. Methods: Eighty patients, 33 women and 47 men, were enrolled in this study. At the end of the surgery, two pieces of 4 cm biodegradable material were applied in each ethmoid cavity. One of them was impregnated with saline solution, while the second one with steroid, or with antibiotic. Results: We observed statistically significant differences in the Lund-Kennedy score between the control and both treatment groups: for the Antibiotic-group on days 10 and 30 (p = 0.009; p = 0.009) and for the Steroid-group on day 90 (p = 0.008). The extended endoscopic appearance of nasal mucosa indicated statistically significant differences in crust formation on day 10 comparing the steroid and control dressing (p = 0.025), in secretion type on days 10 and 30 comparing the antibiotic and control dressing (p = 0.003; p = 0.016) and additionally for steroid and control on day 90 (p = 0.046). On Day 90 we observed statistically significant differences in the absence of mucosal edema in the S-group compared to controls (p = 0.007). Conclusions: The results of this study reveal the significant positive influence of steroid- and antibiotic-impregnated biodegradable nasal packing on the postoperative healing process and patient satisfaction compared to the saline soaked dressing.


Resumo Introdução: A cirurgia endoscópica nasossinusal pode levar à formação de crostas e sinéquias, o que pode afetar o processo de cicatrização. Objetivo: O objetivo do nosso estudo foi comparar a influência do espaçador nasal absorvível embebido em esteroide versus antibiótico versus solução salina na cicatrização de ferida pós-operatória e na satisfação do paciente. Método: Oitenta pacientes, 33 mulheres e 47 homens, foram incluídos neste estudo. Ao final da cirurgia, dois tampões de material biodegradável de 4 cm foram aplicados em cada cavidade etmoidal. Um deles foi embebido em solução salina, enquanto no segundo foi utilizado esteroide, ou antibiótico. Resultados: Observamos diferenças estatisticamente significantes no escore de Lund-Kennedy entre os grupos controle e ambos os grupos tratamentos: para o grupo antibiótico nos dias 10 e 30 (p = 0,009; p = 0,009) e para o grupo esteroide no dia 90 (p = 0,008). O aspecto endoscópico da mucosa nasal indicou diferenças estatisticamente significantes na formação de crostas no dia 10, na comparação do esteroide com o curativo controle (p = 0,025), no tipo de secreção nos dias 10 e 30, na comparação do antibiótico com o curativo controle (p = 0,003; p = 0,016) e adicionalmente para esteroide e controle no dia 90 (p = 0,046). No dia 90, observamos diferenças estatisticamente significantes na ausência de edema da mucosa no grupo E (esteroide) em relação aos controles (p = 0,007). Conclusões: Os resultados deste estudo revelam uma influência positiva significante no uso de tampão nasal biodegradável embebido em esteroides e antibióticos no processo de cicatrização pós-operatória e satisfação do paciente em comparação com o curativo embebido em solução salina.


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Sinusitis/surgery , Steroids/administration & dosage , Bandages , Biocompatible Materials/administration & dosage , Rhinitis/surgery , Anti-Bacterial Agents/administration & dosage , Wound Healing/drug effects , Double-Blind Method , Prospective Studies , Treatment Outcome , Patient Satisfaction , Endoscopy
14.
An. bras. dermatol ; 94(2): 204-210, Mar.-Apr. 2019. tab, graf
Article in English | LILACS | ID: biblio-1001128

ABSTRACT

Abstract This study aims to evaluate tissue healing efficacy in burn patients treated with 1% silver sulfadiazine versus other treatments. This is a systematic literature review and meta-analysis of randomized clinical trials performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyzes (PRISMA) and PICO strategy, registered in the International Prospective Register of Systematic Reviews (PROSPERO) under the number CRD42017081057. The review found 71 studies in MEDLINE/Pubmed, 1 in Clinical Trials, 19 in the Cochrane Library, and 4 in LILACS in five manual searches. Of these, 81 studies were pre-selected. After independent analysis by two reviewers, only 11 studies met the inclusion criteria for the review. All studies (n = 11) using alternative treatments to silver sulfadiazine were shown to be superior in the mean time for complete wound healing, with statistically significant differences between experimental and control groups (p <0.00001); mean difference (- 4.26), 95% CI [- 5.96, - 2.56].


Subject(s)
Humans , Silver Sulfadiazine/therapeutic use , Wound Healing/drug effects , Burns/drug therapy , Anti-Infective Agents, Local/therapeutic use , Silver Sulfadiazine/pharmacology , Time Factors , Randomized Controlled Trials as Topic , Treatment Outcome , Hospitalization , Anti-Infective Agents, Local/pharmacology
15.
Rev. bras. enferm ; 72(1): 200-210, Jan.-Feb. 2019. tab, graf
Article in English | LILACS, BDENF | ID: biblio-990672

ABSTRACT

ABSTRACT Objective: To identify evidence about the effects of growth factor application on venous ulcer healing. Method: Systematic review and meta-analysis, including Randomized Clinical Trials. Searches: Ovid MEDLINE, EMBASE, CINAHL, Cochrane CENTRAL, LILACS, Web of Science, Digital Library of Theses and Dissertations; Google Scholar and list of references. Results: 802 participants were recruited from the 10 included studies: 472 in the intervention group (growth factors) and 330 as control. The relative risk for the complete healing outcome was 1.06 [95% CI 0.92-1.22], p = 0.41. Participants who received Platelet-Rich Plasma and Epidermal Growth Factor showed a slight tendency to achieve complete healing, but without statistical relevance (p <0.05). Most of the studies were classified as moderate risk of bias. Conclusion: The effect of the application of growth factors for complete healing in venous ulcers is not clear, and clinical trials with methodological quality are required for more accurate recommendations.


RESUMEN Objetivo: Identificar evidencias acerca de los efectos de la aplicación de factores de crecimientoenlacicatrización de úlceras venosas. Método: Revisión sistemática y metanálisis, incluyendo Ensayos Clínicos aleatorizados. Búsquedas: Ovid MEDLINE, EMBASE, CINAHL, Cochrane CENTRAL, LILACS, Web of Science, Biblioteca Digital de Tesis y Disertaciones; Google Académico y lista de referencias Resultados: 802 participantes fueron reclutados por los 10 estudios incluidos: 472 en el grupo intervención (factores de crecimiento) y 330 como control. El riesgo relativo para el desenlace de cicatrización completa fue de 1,06 [IC95% 0,92-1,22], p = 0.41. Los participantes que recibieron Plasma Rico en Plaquetas y Factor de Crecimiento Epidérmico presentaron una ligera tendencia a alcanzar una cicatrización completa, pero sin relevancia estadística (p <0.05). La mayoría de los estudios se clasificaron como moderado riesgo de sesgo. Conclusión: El efecto de la aplicación de factores de crecimiento para cicatrización completa en úlceras venosas no está claro, siendo necesarios ensayos clínicos con calidad metodológica para recomendaciones más precisas.


RESUMO Objetivo: Identificar evidências acerca dos efeitos da aplicação de fatores de crescimento na cicatrização de úlceras venosas. Método: Revisão sistemática e metanálise, incluindo Ensaios Clínicos Randomizados. Buscas: Ovid MEDLINE, EMBASE, CINAHL, Cochrane CENTRAL, LILACS, Web of Science, Biblioteca Digital de Teses e Dissertações; Google Acadêmico e lista de referências. Resultados: 802 participantes foram recrutados pelos 10 estudos incluídos: 472 no grupo intervenção (fatores de crescimento) e 330 como controle. O risco relativo para o desfecho de cicatrização completa foi de 1,06 [IC95% 0,92-1,22], p=0.41. Os participantes que receberam Plasma Rico em Plaquetas e Fator de Crescimento Epidérmico apresentaram uma ligeira tendência a alcançar cicatrização completa, porém sem relevância estatística (p<0.05). A maioria dos estudos foi classificada como moderado risco de viés. Conclusão: O efeito da aplicação de fatores de crescimento para cicatrização completa em úlceras venosas não está claro, sendo necessários ensaios clínicos com qualidade metodológica para recomendações mais precisas.


Subject(s)
Humans , Varicose Ulcer/drug therapy , Wound Healing/drug effects , Intercellular Signaling Peptides and Proteins/pharmacology , Randomized Controlled Trials as Topic , Treatment Outcome
16.
Braz. j. med. biol. res ; 52(2): e8209, 2019. tab, graf
Article in English | LILACS | ID: biblio-984033

ABSTRACT

Vegetable oils have been used for a plethora of health benefits by their incorporation in foods, cosmetics, and pharmaceutical products, especially those intended for skin care. This study aimed to investigate the cutaneous benefits of a vegetable oil blend (VOB) formulation and its fatty acid composition. The anti-inflammatory activity was studied in macrophages of RAW 264.7 cells by investigating the release of nitric oxide (NO), superoxide anion generation (O2-), tumor necrosis factor-alpha (TNF-α), and interleukin 6 (IL-6). ABTS cation radical scavenging capacity assay, ferric reducing antioxidant potential (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and NO free radical scavenging assays were used to evaluate the antioxidant activity. VOB was tested for its ability to stimulate fibroblast proliferation and migration using the scratch assay, and antibacterial activity by the microdilution test. The fatty acid profile of a freshly prepared VOB formulation was determined by gas chromatography before and after accelerated stability testing. Chemical composition of VOB revealed the presence of oleic acid (C18:1n-9; 63.3%), linoleic acid (C18:2n-6; 4.7%), and linolenic acid (C18:3n-6; 5.1%) as major mono- and polyunsaturated fatty acids. No changes in the organoleptic characteristics and fatty acid composition were observed after the accelerated stability test. VOB 100 µg/mL reduced the healing time by increasing the total number of cells in the wounded area by 43.0±5.1% compared to the negative control group. VOB also suppressed the pro-inflammatory TNF-α and IL-6 cytokines, and NO and O2- production in lipopolysaccharide-stimulated macrophage cells. In conclusion, the VOB formulation contributed to the improvement of current therapeutic strategies for cutaneous applications in skin care.


Subject(s)
Animals , Rabbits , Wound Healing/drug effects , Plant Oils/pharmacology , Fatty Acids/pharmacology , Anti-Inflammatory Agents/pharmacology , Anti-Bacterial Agents/pharmacology , Antioxidants/pharmacology , Plant Oils/chemistry , Cell Movement/drug effects , Cells, Cultured , Skin Care , Cell Proliferation/drug effects , Fibroblasts/drug effects
17.
Braz. oral res. (Online) ; 33: e035, 2019. tab, graf
Article in English | LILACS | ID: biblio-1001616

ABSTRACT

Abstract: Eugenia dysenterica is a Brazilian tree investigated for its properties and bioactive compounds, which are believed to have both pharmacological and phytochemical therapeutic effects. The leaves of this tree contain tannins, flavonoids, terpenes, and saponins, with reportedly beneficial effects to the human body. Despite these therapeutic applications, its effects have never been tested on oral tissues. Therefore, the aim of the present study was to evaluate the cytotoxic and antioxidant effects and the anti-inflammatory and repair properties of the acetone fraction of E. dysenterica on primary culture of human gingival fibroblasts and on the immortalized murine macrophage cell line (RAW 264.7). For this purpose, a metabolic activity assay, a wound healing assay, a nitric oxide assay, and RT-qPCR were performed. The assays revealed a cytoprotective effect of this plant, suggested by the increase in the expression of SOD1 and NRF2. An antioxidant potential effect was observed in the DPPH• assay. However, the fraction of E. dysenterica did not show anti-inflammatory activity. In conclusion, Eugenia dysenterica may promote cytoprotection when associated with chlorhexidine digluconate because of its antioxidant effect. However, additional studies are necessary on other human dental tissues using other parts of the plant in order to develop a possible mouthwash to assist patients with oral disorders.


Subject(s)
Humans , Animals , Mice , Plant Extracts/pharmacology , Fibroblasts/drug effects , Eugenia/chemistry , Gingiva/chemistry , Anti-Inflammatory Agents/pharmacology , Antioxidants/pharmacology , Reference Values , Time Factors , Wound Healing/drug effects , Brazil , Cells, Cultured , Chlorhexidine/analogs & derivatives , Chlorhexidine/pharmacology , Reproducibility of Results , Plant Leaves/chemistry , Reverse Transcriptase Polymerase Chain Reaction , RAW 264.7 Cells , Nitric Oxide/analysis
18.
Braz. j. med. biol. res ; 52(9): e8290, 2019. graf
Article in English | LILACS | ID: biblio-1019570

ABSTRACT

Tendon rupture is a very frequent accident involving average people and high-performance athletes. Clinical studies describe tendon recovery as a painful and slow process involving different biochemical and histological events. Ascorbic acid (AA) is a potent antioxidant as well as an important cofactor for collagen synthesis. In the current study, we evaluated if local treatment with AA is able to promote tendon repair in tenotomized rats. Animals were submitted to Achilles tendon rupture followed by surgical suture. Control and AA groups received in loco injection of saline solution (0.9% NaCl) and 30 mM AA, respectively. Histological and functional recovery of Achilles tendon tissue was evaluated at 7, 14, and 21 days post-surgery. Hematoxylin/eosin staining and collagen fluorescence analysis showed intense disarrangement of tendon tissue in the saline group. Tenotomized animals also showed hypercellularity in tendon tissue compared with non-tenotomized animals. The Achilles functional index (AFI) showed a significant decrease of tendon functionality in tenotomized animals at 7, 14, and 21 days post-surgery. AA accelerated tissue organization and the recovery of function of the Achilles tendons. The beneficial effect of AA treatment was also observed in the organization of the collagen network. Data presented in the current work showed that in loco treatment with AA accelerated the recovery of injured Achilles tendon post-surgery.


Subject(s)
Animals , Male , Rats , Ascorbic Acid/administration & dosage , Achilles Tendon/drug effects , Tendon Injuries/surgery , Collagen/drug effects , Achilles Tendon/injuries , Achilles Tendon/pathology , Wound Healing/drug effects , Collagen/physiology , Rats, Wistar , Recovery of Function/drug effects , Disease Models, Animal , Tenotomy
19.
J. appl. oral sci ; 27: e20180015, 2019. graf
Article in English | LILACS, BBO | ID: biblio-975889

ABSTRACT

Abstract In this study, the effects of ozonetherapy on secondary wound healing were evaluated histologically and immuno-histochemically. Material and Methods: 8 healthy pigs were used in this study. Six wounds with 10 mm in diameter were created through the punch technique on the palatinal gingiva of each pig. Ozone gas was applied on only 3 wounds (test group) and the remaining 3 were left to natural healing (control group). Biopsy samples were taken from one of the wounds in each group on the third day, from another wound of each group on the seventh day, and from another one on the tenth day. Routine histological analysis and immuno-histochemical staining were performed to investigate transforming growth factor-beta (TGF-β) and (VEGF) expressions. Results: No statistical difference was found between the test and control groups in terms of collagen fibers, epithelial formation and inflammation scores. A VEGF expression found in the test group was statistically higher than control group samples taken on the 3rd and 7th day. There was no statistical difference between the test and control groups in terms of TGF-β expression on any of the sampling days. Conclusion: The topical application of ozone gas could be effective in the early stages of wound healing by increasing the amount of VEGF expression. Clinical Relevance: Topical application of ozone gas may be effective in the early stages of oral wound healing.


Subject(s)
Animals , Ozone/therapeutic use , Wound Healing/drug effects , Gingiva/drug effects , Gingiva/pathology , Reference Values , Swine , Time Factors , Biopsy , Immunohistochemistry , Random Allocation , Reproducibility of Results , Administration, Topical , Transforming Growth Factor beta/analysis , Transforming Growth Factor beta/drug effects , Treatment Outcome , Vascular Endothelial Growth Factor A/analysis , Vascular Endothelial Growth Factor A/drug effects
20.
Acta cir. bras ; 34(1): e20190010000004, 2019. tab, graf
Article in English | LILACS | ID: biblio-983681

ABSTRACT

Abstract Purpose: To characterize qualitatively and quantitatively the absorption of metronidazole solution, in greater concentrations and for longer periods, when applied topically to an experimental open skin wound model. Methods: An open skin wound, 2 cm in diameter and total skin thickness was prepared, under anesthetic, in the dorsal region of 108 Wistar rats weighing between 300 and 350 grams. The animals were allocated to groups of 18 animals in accordance with the concentration of metronidazole in the solution to be applied daily to the wound. In the control group (CG), 0.9% sodium chloride solution was used for application, and in the experimental groups (GI, GII, GIII, GIV and GV) metronidazole solution at 4%, 6%, 8%, 10% and 12%, respectively, was applied. After 3, 7 and 14 days of treatment. Blood samples collected through cardiac puncture were examined for the existence or non-existence of metronidazole, using high performance liquid chromatography (HPLC). Detected metronidazole values were compared statistically within each group (temporal analysis 3 days X 7 days X 14 days) and between the groups that used topical metronidazole (4% X 6% X 8% X 10% and 12%) using the Kruskal-Wallis test, considering a statistical significance of 95% (p<0.05). Results: Metronidazole was detected in all the samples at all times in all the groups in which topical metronidazole was applied to the wounds. Characteristically, there was no significant difference between the doses obtained within each group over time (3 days X 7 days X 14 days) GI=0.461; GII=0.154; GIII=0.888; GIV= 0.264 and GV=0.152. In the evaluation between groups, a similar degree of absorption was found after 3 days (p=0.829) and 14 days (p=0.751). Conclusion: The serum concentration of metronidazole that was achieved was not influenced by the concentration of the solution applied to the skin wound, with similar extend, or by the duration of the application.


Subject(s)
Animals , Male , Rats , Wound Healing/drug effects , Metronidazole/administration & dosage , Metronidazole/blood , Anti-Infective Agents/administration & dosage , Anti-Infective Agents/blood , Time Factors , Administration, Topical , Chromatography, Liquid , Rats, Wistar , Disease Models, Animal
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