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1.
Int. j. morphol ; 42(4): 929-935, ago. 2024. ilus
Article in Spanish | LILACS | ID: biblio-1569255

ABSTRACT

Los salmónidos, durante su desarrollo, pueden experimentar ocasionalmente deformaciones esqueléticas. Para su diagnóstico se emplean diversas metodologías, entre las que se incluyen radiografías, técnicas histológicas, diafanización con tinciones de alizarina y azul de alcián, así como el uso del microscopio electrónico de barrido (SEM), cada una con sus inherentes ventajas y desventajas. Este estudio tuvo como finalidad evaluar y comparar la eficacia de la tomografía microcomputarizada (Micro-CT) para el análisis anatómico, reconstruyendo tridimensionalmente las imágenes y contrastándolas con los resultados obtenidos mediante la técnica de diafanización. Se analizaron las aletas caudales de cinco ejemplares de salmón Oncorhynchus kisutch: dos sujetos a diafanización y tres procesados para análisis mediante Micro-CT utilizando el equipo BRUKER SkyScan 1272. La técnica de Micro-CT demostró superioridad en la resolución de las estructuras óseas, facilitando una exploración detallada de las variaciones morfológicas y la distribución de la densidad mineral. Este enfoque permitió identificar anomalías en la morfología y crecimiento de las últimas vértebras y lepidotriquias dorsales, así como una densidad incrementada en lepidotriquias dorsales malformadas. La mayor resolución proporcionada por la Micro-CT no solo potencia nuestra comprensión de la ontogenia piscícola y su adaptación a ambientes diversos, sino que además inaugura perspectivas innovadoras para el estudio de la evolución de las estrategias locomotoras y las respuestas adaptativas frente a cambios ambientales a través del tiempo.


SUMMARY: During their development, some species of salmonids may occasionally experience skeletal deformations. Several methodologies are currently being used for the diagnosis of such malformations, among which X-rays, histological techniques, diaphanization coupled either with Alizarin Red or Alcian Blue stains, as well as Scanning Electron Microscopy (SEM) can be mentioned. Each one of those methods presents inherent advantages and disadvantages. The purpose of this study was twofold: Firstly, to evaluate and compare the effectiveness of microcomputed tomography (Micro-CT) technology for anatomical analysis, three-dimensionally reconstructing the obtained images; and secondly, to contrast those images with the results obtained through the diaphanization technique. The caudal fins of five specimens of the Oncorhynchus kisutch salmon were analyzed: Two specimens were subjected to diaphanization and three were processed for Micro-CT analysis, using the BRUKER SkyScan 1272 equipment. The Micro-CT technology demonstrated superiority in the resolution of bone structures, facilitating a detailed exploration of morphological variations, as well as the distribution of mineral density. This experimental approach allowed us to identify anomalies in the morphology and growth of the last vertebrae and dorsal lepidotrichiae, as well as an increased mineral density in the malformed dorsal lepidotrichiae. The higher resolution provided by Micro-CT not only enhances our understanding of the fish ontogeny and its adaptation to diverse environments, but also opens innovative perspectives for the study of the evolution of locomotor strategies and adaptive responses to environmental changes.


Subject(s)
Animals , Oncorhynchus kisutch/anatomy & histology , X-Ray Microtomography/methods , Salmonidae/anatomy & histology , Bone and Bones/diagnostic imaging , Bone Density , Imaging, Three-Dimensional , Animal Fins/diagnostic imaging
2.
Int. j. morphol ; 42(1): 28-34, feb. 2024. ilus, tab
Article in English | LILACS | ID: biblio-1528823

ABSTRACT

SUMMARY: This work investigated the morphology of the root canal system of the mandibular first molar in a Malaysian subpopulation. Using micro-computed tomography with an isotropic resolution of 22 µm, 140 mandibular first molars were scanned. MIMICS software was used for segmentation, 3-D reconstruction and analysis of the acquired images. The canal configuration was described using Vertucci [supported by the supplementary configurations proposed by Sert & Bayirli (2004)] and Ahmed et al. (2027), coding systems. The chi-square test was used to assess the association between qualitative variables. By non-considering intercanal communications, Vertucci types IV (17.1%) and I (76.4%) were the most frequently reported configurations in the mesial and distal roots, respectively. Of the reported configurations, 24.3% and 4.3% were non-classifiable by Vertucci system in the mesial and distal roots, respectively. Up to 63.6% and 9.3% of the reported configurations were non- classifiable, and type I was the most frequent when considering intercanal communications (7.1% and 76.4% in the mesial and distal roots, respectively). According to Ahmed et al., system, almost half of the sample had more than four digits (47.9%), followed by the 3-digits category (20.71%). In both systems, a significant association was found between the canal configuration and the root type (p<0.001). The mandibular first molar of this Malaysian subpopulation demonstrated a wide range of root canal morphology. When compared to the Vertucci system, the system developed by Ahmed et al., successfully classified all molars configurations despite their level of complexity. The complex canal anatomy of mandibular first molars in this subpopulation warrants special attention during root canal treatment procedures.


En este trabajo se investigó la morfología del sistema de conductos radiculares del primer molar mandibular en una subpoblación de Malasia. Utilizando tomografía microcomputada con una resolución isotrópica de 22 µm, se escanearon 140 primeros molares mandibulares. Se utilizó el software MIMICS para segmentar (enmascarar), reconstruir en 3D, visualizar y analizar las imágenes adquiridas. La configuración del canal se describió utilizando Vertucci respaldado por las configuraciones complementarias propuestas por Sert & Bayirli (2004)] y Ahmed et al. (2017, 2020), sistemas de codificación. Se utilizó la prueba de chi-cuadrado para evaluar la asociación entre variables cualitativas. Sin considerar las comunicaciones intercanales, los tipos Vertucci IV (17,1%) y I (76,4%) fueron las configuraciones reportadas con mayor frecuencia en las raíces mesiales y distales, respectivamente. De las configuraciones reportadas, el 24,3 % y el 4,3 % fueron no clasificables por el sistema de Vertucci en las raíces mesial y distal, respectivamente. Hasta el 63,6 % y el 9,3 % de las configuraciones reportadas fueron no clasificables, siendo la tipo I la más frecuente al considerar las comunicaciones intercanales (7,1 % y 76,4 % en las raíces mesiales y distales, respectivamente). Según Ahmed et al. (2017, 2020) en el sistema, casi la mitad de la muestra tenía más de cuatro dígitos (47,9 %), seguido por la categoría de 3 dígitos (20,71 %). En ambos sistemas se encontró una asociación significativa entre la configuración del canal y el tipo de raíz (p<0,001). El primer molar mandibular de esta subpoblación de Malasia demostró una amplia gama morfológica del conducto radicular. En comparación con el sistema Vertucci, el sistema desarrollado por Ahmed et al. (2017, 2020) clasificaron con éxito todas las configuraciones de los molares a pesar de su nivel de complejidad. La compleja anatomía del canal de los primeros molares mandibulares en esta subpoblación merece una atención especial durante los procedimientos de tratamiento de conducto.


Subject(s)
Humans , Dental Pulp Cavity/diagnostic imaging , X-Ray Microtomography , Molar/diagnostic imaging , Dental Pulp Cavity/anatomy & histology , Malaysia , Molar/anatomy & histology
3.
Rev. Fac. Odontol. (B.Aires) ; 39(91): 41-48, 2024. ilus
Article in Spanish | LILACS | ID: biblio-1554951

ABSTRACT

La microtomografía es un estudio que utiliza la ra-diación X para obtener imágenes de tamaños de mi-lésimas de milímetros y de alta resolución. Las imá-genes 2D son procesadas por diferentes softwares para lograr obtener volúmenes capaces de ser ana-lizados tridimensionalmente. La microtomografía es el estudio de elección a la hora de evaluar caracte-rísticas muy pequeñas con gran precisión. La obtu-ración endodóntica buscar lograr un sellado que no tenga espacios vacíos dentro de la masa de obtura-ción. Esto es importante debido a que los poros pue-den permitir, si están en contacto con la pared den-tinaria, la entrada de microorganismos al conducto radicular. El objetivo de este trabajo fue describir el procedimiento para el análisis y visualización de los espacios vacíos dentro de la obturación endodónti-ca, utilizando la microtomografía de rayos x, y esta-blecer un protocolo para ser utilizado por cualquier investigador(AU)


Microtomography is a study that uses X-radiation to obtain high-resolution images of sizes of thousandths of millimeters. The 2D images are processed by different software to obtain volumes capable of being analyzed three-dimensionally. Microtomography is the study of choice when evaluating very small features with great precision. Endodontic filling seeks to achieve a seal that does not have voids within the filling obturation. This is important because the voids can allow, if they are in contact with the dentin wall, the entry of microorganisms into the root canal. The objective of this work was to describe the procedure for the analysis and visualization of voids within the endodontic filling using microtomography and to establish the protocol to be used by any researcher (AU)


Subject(s)
Root Canal Obturation/adverse effects , Clinical Protocols , X-Ray Microtomography/methods , Porosity , Imaging, Three-Dimensional/methods , Dental Leakage/prevention & control
4.
Braz. dent. sci ; 27(2): 1-8, 2024. ilus, tab
Article in English | LILACS, BBO | ID: biblio-1567092

ABSTRACT

Objective: This study evaluated different methods of calcium hydroxide (CH) removal from root canals with simulated internal resorptions using microcomputed tomography (micro-CT). Material and Methods: Sixty acrylic resin blocks with simulated root canals and internal resorptions were prepared using a Reciproc R25 file and then filled with CH. The blocks were divided into five test groups (n=12) according to the method used for CH removal: hand files (HF), Easy Clean (EC), passive ultrasonic irrigation (PUI), XP-Endo Finisher (XP), XP-Endo Finisher + PUI (XP+PUI). The blocks were scanned using a SkyScan 1172 scanner before and after CH removal to measure the volume and percentage of CH removal. The OriginPro 2017 software was used for statistical analyses. The level of significance was set at p<0.05 for all tests. Results: No method under study removed all CH. All methods had similar results in the cervical third (P>0.05). The percentage of CH removal was significantly greater in the area of internal resorption and along the total length of the canal in the XP+PUI group (P<0.05). The best results of CH removal were found in the apical third of roots in the XP+PUI and PUI groups (P>0.05). Conclusion: No method removed all CH from the root canals, but the combined XP+PUI method removed more CH than the other methods, especially from the area of the internal resorption(AU)


Objetivo: Este estudo avaliou diferentes métodos de remoção de hidróxido de cálcio (CH) de canais radiculares com reabsorções internas simuladas por meio de microtomografia computadorizada (micro-CT). Material e Métodos: Sessenta blocos de resina acrílica com canais radiculares simulados e reabsorções internas foram preparados com lima Reciproc R25 e posteriormente preenchidos com CH. Os blocos foram divididos em cinco grupos de teste (n=12) de acordo com o método utilizado para remoção de CH: limas manuais (HF), Easy Clean (EC), irrigação ultrassônica passiva (PUI), XP-Endo Finisher (XP), XP -Endo Finalizador + PUI (XP + PUI). Os blocos foram escaneados usando um scanner SkyScan 1172 antes e depois da remoção do CH para medir o volume e a porcentagem de remoção do CH. O software OriginPro 2017 foi utilizado para análises estatísticas. O nível de significância foi estabelecido em p<0,05 para todos os testes. Resultados: Nenhum método em estudo removeu todos o CH. Todos os métodos tiveram resultados semelhantes no terço cervical (P>0,05). A porcentagem de remoção de CH foi significativamente maior na área de reabsorção interna e ao longo do comprimento total do canal no grupo XP+PUI (P<0,05). Os melhores resultados de remoção de CH foram encontrados no terço apical das raízes nos grupos XP+PUI e PUI (P>0,05). Conclusão: Nenhum método removeu todo o CH dos canais radiculares, mas o método combinado XP+PUI removeu significativamente mais CH do que os outros métodos, especialmente da área de reabsorção interna (AU)


Subject(s)
Tooth Resorption , Calcium Hydroxide , Dental Instruments , Dental Pulp Cavity , X-Ray Microtomography
5.
Odovtos (En línea) ; 25(3): 32-42, Sep.-Dec. 2023. tab, graf
Article in English | LILACS, BBO, SaludCR | ID: biblio-1529067

ABSTRACT

Abstract The aim of this study was to compare the filling capacity in curved root canal using a new continuous wave of condensation technique (Termo Pack II, Easy Dental Equipments, Brazil) or lateral compaction. The percentage of voids in the filling of mesial root canals of mandibular molars was assessed by micro-computed tomography (micro-CT). Mesial root canals (n=24) of mandibular molars with a degree of curvature between 20° and 40° were prepared using rotary system (ProDesign Logic, Easy, Brazil) up to #35, .05 taper. The root canals were filled by using the continuous wave of condensation system or lateral compaction and AH Plus sealer (n=12). Scanning at 9 µm was performed after preparation and after filling by using micro-CT SkyScan 1176. The volumetric percentage of filling material and voids (total length and in each root canal third) were calculated. Data were analyzed using ANOVA/ Tukey and Student's t tests (α=0.05). Before the filling techniques, the root canals volume after preparation was similar (p>0.05). The root canals filled by the continuous wave of condensation technique presented the lowest percentage of voids, and the greatest percentage of filling material in total length and thirds (cervical, middle and apical) (p<0.05). Both techniques were not able of completely filling the root canals. The continuous wave of condensation technique Termo Pack II promoted better root canal filling in curved root canals, when compared with lateral compaction.


Resumen El objetivo de este estudio fue comparar la capacidad de obturación en conductos radiculares curvos utilizando una nueva técnica de condensación de onda continua (Termo Pack II, Easy Dental Equipments, Brasil) vs compactación lateral. El porcentaje de brechas en la obturación de los conductos radiculares mesiales de los molares mandibulares se evaluó mediante microtomografía computarizada (micro-CT). Se prepararon conductos radiculares mesiales (n=24) de molares mandibulares con un grado de curvatura entre 20° y 40° utilizando un sistema rotatorio (ProDesign Logic, Easy, Brasil) al #35, conicidad 0,05. Los conductos radiculares se obturaron utilizando un sistema de condensación de onda contínua o compactación lateral y cemento AH Plus (n=12). Se realizó un escaneo de 9 µm después de la preparación y después de la obturación usando el micro-CT SkyScan 1176. Se calculó el porcentaje volumétrico de material de obturación y vacíos (longitud total y en cada tercio del conducto radicular). Los datos se analizaron utilizando las pruebas ANOVA/Tukey y t de Student (α=0,05). Antes de las técnicas de obturación, el volumen de los conductos radiculares después de la preparación fue similar (p>0,05). Los conductos radiculares obturados con la técnica de condensación por onda contínua presentaron el menor porcentaje de vacíos y el mayor porcentaje de material de obturación en longitud total y en tercios (cervical, medio y apical) (p<0,05). Ambas técnicas no fueron capaces de llenar completamente los conductos radiculares. La técnica de condensación de onda contínua Termo Pack II promovió un mejor relleno del conducto radicular en conductos radiculares curvos en comparación con la compactación lateral.


Subject(s)
Root Canal Obturation/instrumentation , Condensation , Dental Pulp , X-Ray Microtomography/instrumentation
6.
Article in Chinese | WPRIM | ID: wpr-1009206

ABSTRACT

OBJECTIVE@#To explore effects of isopsoralen (ISO) with different doses on fracture and vascular healing in mice.@*METHODS@#Sixty 2-month-old male C57BL/6 mices with body mass of (20±2) g were selected and divided into 4 groups by random number table method:model group (model), low dose group (isopsoralen-low dose, ISO-L), medium dose group (isopsoralen-medium dose, ISO-M) and high dose group (isopsoralen-high dose, ISO-H), with 15 animals in each group. The right tibial fracture model was established. After operation, ISO-L group, ISO-M group and ISO-H group were given ISO concentration of 10 mg·kg-1, 20 mg·kg-1 and 40 mg·kg-1, respectively. Model group was given same volume of normal saline once a day for 28 days. Weighed once a week. X-ray was performed on 7, 14, 21 and 28 days, respectively, and modified I.R. Garrett scoring method was used to evaluate callus growth. After 28 days, the main organs were stripped and weighed, and organ coefficients were calculated. Hematoxylin eosin staining (HE staining) was performed on the organs to observe whether there were pathological structural changes. Micro-computed tomography (Micro-CT) was used to scan fracture area and conduct three-dimensional reconstruction to obtain the effect map, and quantify bone volume fraction (bone volume/total volume, BV/TV). After decalcification, the tibia was embedded in paraffin wax and sectioned. The healing and shape of fracture end were observed by HE staining and ferruxin solid green staining. The right tibia was removed and decalcified after intravascular infusion of Microfil contrast agent. Micro-CT was used to scan the callus microvessels in the fracture area, and the vascular volume fraction and vessel diameter were quantified.@*RESULTS@#After 28 days of administration, there was no significant difference in body mass and organ coefficient among all groups (P>0.05), and no significant pathological changes were found in HE staining of organs. The results of X-ray and improved I.R. Garrett score showed that ISO-M group was higher than that of Model group at 28 days (P<0.05). Scores of ISO-H group at 14, 21 and 28 days were higher than those of the other 3 groups (P<0.05). Micro-CT results showed intracavitary callus in ISO-M group was significantly reduced, which was lower than that in Model group (P<0.05), most of the callus in ISO-H group were subsided, and BV/TV in ISO-H group was lower than that in the other 3 groups (P<0.05). The results of HE staining and ferrubens solid green staining showed fracture area of ISO-H group was closed, continuous laminar bone had appeared, and the fracture healing process was higher than that of other groups. Angiographic results showed vascular volume fraction in ISO-H and ISO-M groups was higher than that in Model and ISO-L groups (P<0.05), and the vascular diameter in ISO-H and ISO-M groups was higher than that in Model and ISO-L groups (P<0.05).@*CONCLUSION@#In the concentration range of 10-40 mg·kg-1, ISO has no obvious toxic and side effects, and could improve bone microstructure, promote formation of callus microvessels, and accelerate healing of fracture ends in a concentration-dependent manner.


Subject(s)
Mice , Male , Animals , X-Ray Microtomography , Mice, Inbred C57BL , Bony Callus , Fracture Healing , Tibial Fractures/surgery
7.
Article in English | WPRIM | ID: wpr-1010712

ABSTRACT

Recent studies have suggested that long-term application of anti-angiogenic drugs may impair oral mucosal wound healing. This study investigated the effect of sunitinib on oral mucosal healing impairment in mice and the therapeutic potential of Bifidobacterium breve (B. breve). A mouse hard palate mucosal defect model was used to investigate the influence of sunitinib and/or zoledronate on wound healing. The volume and density of the bone under the mucosal defect were assessed by micro-computed tomography (micro-CT). Inflammatory factors were detected by protein microarray analysis and enzyme-linked immunosorbent assay (ELISA). The senescence and biological functions were tested in oral mucosal stem cells (OMSCs) treated with sunitinib. Ligated loop experiments were used to investigate the effect of oral B. breve. Neutralizing antibody for interleukin-10 (IL-10) was used to prove the critical role of IL-10 in the pro-healing process derived from B. breve. Results showed that sunitinib caused oral mucosal wound healing impairment in mice. In vitro, sunitinib induced cellular senescence in OMSCs and affected biological functions such as proliferation, migration, and differentiation. Oral administration of B. breve reduced oral mucosal inflammation and promoted wound healing via intestinal dendritic cells (DCs)-derived IL-10. IL-10 reversed cellular senescence caused by sunitinib in OMSCs, and IL-10 neutralizing antibody blocked the ameliorative effect of B. breve on oral mucosal wound healing under sunitinib treatment conditions. In conclusion, sunitinib induces cellular senescence in OMSCs and causes oral mucosal wound healing impairment and oral administration of B. breve could improve wound healing impairment via intestinal DCs-derived IL-10.


Subject(s)
Animals , Mice , Interleukin-10 , Bifidobacterium breve , Up-Regulation , Angiogenesis Inhibitors , Sunitinib , X-Ray Microtomography , Administration, Oral , Wound Healing , Antibodies, Neutralizing
8.
Pesqui. bras. odontopediatria clín. integr ; 23: e210162, 2023. tab, graf
Article in English | LILACS, BBO | ID: biblio-1431047

ABSTRACT

ABSTRACT Objective: To compare the agreement of images in white light (WL), fluorescence (FL), and digital radiographs (DR), on the diagnosis and treatment decisions for occlusal caries lesions against a micro-CT gold standard. Material and Methods: Ten extracted third molars, with enamel and/or dentin caries (ICDAS 2-4), were included. Occlusal surface images were acquired with an intraoral camera (SoproLife®) in WL and FL modes. DR was obtained using an intraoral X-ray and a semi-direct digital system. A total of 780 images were needed, organized in a template, to be later examined by twenty-six dentists invited to compose the study. The Generalized Estimation Equations model was used to compare the proportions of the correct answers between the three methods and the gold standard. When significant, Bonferroni post-hoc test was used to identify differences (α=5%). Results: Most of the examiners were specialists (76.9%) with 14.5 years of experience. All diagnostic methods were similar and showed low agreement (DR 12.7%, WL 16.5%, and FL 16.5%) compared with gold standard caries diagnostic scores. Regarding treatment decisions, mean agreement for all diagnostic methods was higher (43.2%; p<0.001), and among all methods, WL (48.1%) and FL (51.2%) modes performed better than DR (30.4%, p<0.001). Conclusion: SoproLife® images could help clinicians to propose rational, minimally invasive treatments for occlusal caries lesions.


Subject(s)
Humans , Dental Caries/diagnostic imaging , X-Ray Microtomography/methods , Fluorescence , Clinical Decision-Making , Molar, Third/injuries , Effectiveness , In Vitro Techniques/methods , Cross-Sectional Studies/methods , Radiography, Dental, Digital/methods
9.
Braz. j. oral sci ; 22: e231303, Jan.-Dec. 2023. ilus
Article in English | LILACS, BBO | ID: biblio-1442844

ABSTRACT

Aim: To evaluate the potential of inducing mineral density changes of indirect pulp capping materials applied to demineralized dentin. Methods: A total of 50 cavities were prepared, 5 in each tooth, in extracted ten molars without caries, impacted or semi-embedded. The cavities were scanned by microcomputed tomography (µ-CT) after creating artificial caries by microcosm method (pre-treatment). Each cavity was subjected to one of 5 different experimental conditions: control (dental wax), conventional glass ionomer cement (Fuji IX GP Extra), resin-modified calcium silicate (TheraCal LC), resin-modified calcium hydroxide (Ultra-Blend Plus), MTA (MM-MTA) and the samples were kept under intrapulpal pressure using simulated body fluid for 45 days. Then, the second µ-CT scan was performed (post-treatment), and the change in dentin mineral density was calculated. Afterward, elemental mapping was performed on the dentinal surfaces adjacent to the pulp capping agents of 5 randomly selected samples using energy dispersive X-ray spectroscopy (EDS) apparatus attached to a scanning electron microscope (SEM). The Ca/P ratio by weight was calculated. Friedman test and Wilcoxon Signed Ranks test were used to analyze the data. Results: There was a significant increase in mineral density values of demineralized dentin after treatment for all material groups (p<0.05). Resin-modified calcium silicate had similar efficacy to MTA and conventional glass ionomer cement, but was superior to resin-modified calcium hydroxide in increasing the mineral density values of demineralized dentin. Conclusions: Demineralized dentin tissue that is still repairable can be effectively preserved using materials with remineralization capability


Subject(s)
Spectrometry, X-Ray Emission , Calcium Hydroxide , Silicates , Calcium Compounds , X-Ray Microtomography , Glass Ionomer Cements
10.
Araçatuba; s.n; 2023. 78 p. ilus, graf.
Thesis in Portuguese | LILACS, BBO | ID: biblio-1553304

ABSTRACT

O ozônio tem sido utilizado no processo de reparo ósseo em condições desfavoráveis, como na osteonecrose dos maxilares induzida por medicamentos (OMIM). O objetivo deste trabalho foi avaliar a aplicação de ozonioterapia como prevenção e/ou o tratamento da OMIM. Cento e vinte ratos wistar foram distribuídos entre os grupos tratamentos: eles foram induzidos com Zoledronato e receberam ozonioterapia antes da exodontia (prevenção ­ GOP), após exodontia (tratamento ­ GOT), em ambos momentos (prevenção e tratamento ­ GOPT), um grupo não recebeu ozônio (ZOL), e outro recebeu soro fisiológico ao invés da indução (SAL). Após 14 e 28 dias pós-operatórios foram eutanasiados e as peças submetidas as análises histológicas com eosina e hematoxilina, imunoistoquímica, microtomográfica computadorizada (microCT), confocal a LASER e histomorfométrica. Em 14 dias observamos o início do reparo em GOT, assim como um epitélio presente em SAL e GOT, o mesmo ocorre em 28 dias, e uma intensa imunomarcação de osteocalcina (OC) em GOPT em 14 dias. A microCT demostrou maiores médias de BV/TV em todos grupos quando comparados a ZOL (p< 0,05), seguido pelo grupo SAL (66,039±28,379) e ZOL (60,856±28,425). Diante dos resultados pode-se observar que a ozonioterapia pode modular o reparo alveolar em animais induzidos com ácido zoledrônico(AU)


Ozone has been used in the bone repair process under unfavorable conditions, such as in druginduced osteonecrosis of the jaws (OMIM). The objective of this work was to evaluate the application of ozone therapy as prevention and/or treatment of OMIM. One hundred and twenty wistar rats were distributed among treatment groups: they were induced with Zoledronate and received ozone therapy before tooth extraction (prevention - GOP), after tooth extraction (treatment - GOT), at both times (prevention and treatment - GOPT), one group I did not receive ozone (ZOL), and another received saline instead of induction (SAL). After 14 and 28 days postoperatively, they were euthanized and the pieces submitted to histological analysis with eosin and hematoxylin, immunohistochemistry, computed microtomography (microCT), confocal LASER and histomorphometric analysis. In 14 days we observed the beginning of repair in GOT, as well as an epithelium present in SAL and GOT, the same occurs in 28 days, with intense immunostaining of osteocalcin (OC) in GOPT in 14 days. The microCT showed higher BV/TV means in all groups when compared to the ZOL (p< 0.05), followed by the SAL group (66.039±28.379) and ZOL (60.856±28.425). In view of the results, it can be observed that ozone therapy can modulate alveolar repair in animals induced with zoledronic acid(AU)


Subject(s)
Animals , Rats , Bisphosphonate-Associated Osteonecrosis of the Jaw , Bisphosphonate-Associated Osteonecrosis of the Jaw/prevention & control , Bisphosphonate-Associated Osteonecrosis of the Jaw/therapy , Tooth Socket , X-Ray Microtomography , Zoledronic Acid
11.
Braz. j. oral sci ; 22: e231400, Jan.-Dec. 2023. ilus
Article in English | LILACS, BBO | ID: biblio-1524336

ABSTRACT

Aim: This study aimed to assess the shaping ability of Reciproc Blue in the apical third and apical foramen of moderately curved canals at different working lengths (WLs), by micro-computed tomography. Methods: Thirty-six mesial roots (mesiobuccal and mesiolingual canals) were included, each with 2 separate root canals and independent apical foramina, according to type IV of Vertucci's classification of first and second mandibular molars. The canals were instrumented at three different WLs: G-1, 1mm short of the major apical foramen; G0, at the major apical foramen; G+1, 1mm beyond the major apical foramen. The groups were assessed for changes in root canal volume and untouched wall area in the apical third. Groups G0 and G+1 were also compared for percentage of untouched walls at the apical foramen. One-way ANOVA (post hoc Tukey test) and Student's t-test adopted a 5% level of significance. Results: Root canal volumes (mm3) in the apical third were 22.86±10.46, 44.48±24.91, and 55.71±21.32 in G-1, G0 and G+1, respectively. G-1 volume following instrumentation increased significantly less than that of G0 or G+1 (P>.05); G0 did not differ from G+1. The percentage of untouched wall area in the apical third did not differ among the three groups (P>.05). G0 and G+1 did not differ regarding untouched walls in the major apical foramem walls. Conclusion: Extending the WL from 1mm short of the apical foramen to a point at and beyond the WL increases the apical third volume without increasing the prepared area. Untouched surface areas of the apical foramen were not modified by instrumentation at or beyond the foramen


Subject(s)
Periapical Periodontitis , Root Canal Therapy , Tooth Apex , Dental Pulp Cavity , X-Ray Microtomography
12.
Article in Chinese | WPRIM | ID: wpr-935829

ABSTRACT

Objective: To investigate the effects of straight-line minimally invasive access cavity on the mechanical properties of endodontically treated maxillary first premolars using finite element analysis. Methods: Micro-CT data of twenty maxillary first premolars were collected for three-dimensional reconstruction. Three access cavities, including the conventional access cavity (ConvAC), the truss access cavity (TrussAC) and the straight-line minimally invasive access cavity (SMIAC), as well as the root canal treatment procedure, were simulated in all the 20 reconstruction samples of three-dimensional models, respectively. The peak von Mises stress on the cervical area of each model, as well as the stress distribution under vertical and oblique loading circumstances, were subsequently determined by using finite element analysis. Results: In comparison to the stresses of ConvAC [buccal cervical (BC): (188.7±13.4) MPa, palatal cervical (PC): (200.9±25.7) MPa], the stresses of TrussAC [BC: (146.0±12.9) MPa, PC: (167.6±15.9) MPa] (t=9.01, P<0.001; t=4.59, P<0.001) and SMIAC [BC: (142.6±13.7) MPa, PC: (168.1±17.4) MPa] (t=9.64, P<0.001; t=3.76, P=0.004) significantly reduced the peak von Mises stress on the cervical area of the maxillary first premolars after root canal treatment. Under vertical loading conditions, SMIAC also reduced the central tendency of stresses on the occlusal surface, cervical area and root. In the case of oblique loading conditions, similar results were observed. Under both loading conditions, there was no significant difference in the peak von Mises stress on the cervical area of the maxillary first premolar between TrussAC and SMIAC groups. Conclusions: The design of SMIAC could preserve the mechanical properties of the maxillary first premolar following root canal treatment, which might have certain clinical feasibility.


Subject(s)
Bicuspid , Dental Stress Analysis , Finite Element Analysis , Root Canal Therapy , Stress, Mechanical , X-Ray Microtomography
13.
Article in English | WPRIM | ID: wpr-939911

ABSTRACT

Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease. It is known that aucubin (AU) exerts anti-inflammatory activity, but its effects and mechanisms in RA are unclear. This study investigated the anti-inflammatory effects and mechanisms of AU in vivo and in vitro. Human fibroblast-like synoviocyte cells from patients with RA (HFLS-RA), RAW264.7 cells, and MC3T3-E1 cells were used to evaluate the effects of AU on migration, invasion, apoptosis, osteoclast differentiation and production. Immunofluorescence was used to observe nuclear translocation of nuclear factor (NF)-κB, the double luciferase reporter gene method was used to observe NF-κB-p65 activity in AU-treated MC3T3-E1 cells. RT-qPCR was used to measure expression of bone metabolism and inflammation-related genes, and western blot was used to measure bone metabolism and NF-κB protein expression levels. Collagen-induced arthritis (CIA) rat model was used for pharmacodynamics study. Arthritis indexes were measured in the ankle and knee, histological staining and Micro-computed tomography were performed on the ankle joints. Also, inflammatory factor gene expression and the levels of NF-κB-related proteins were detected as in vitro. AU effectively inhibited HFLS-RA cell migration and invasion, promoted apoptosis, and inhibited RAW264.7 cell differentiation into osteoclasts, as well as inhibited NF-κB-p65 activity in MC3T3-E1 cells. Notably, AU significantly reduced the gene expression levels of three cell-related inflammatory factors and bone metabolism factors, effectively inhibited the expression of p-Iκκα β, p-IκBα, and p-p65 proteins. In vivo, AU relieved joint inflammation, reduced related inflammatory factors, and inhibited NF-κB signaling. It could be used to treat RA-related synovial inflammation and bone destruction through the NF-κB pathway.


Subject(s)
Animals , Humans , Rats , Anti-Inflammatory Agents/therapeutic use , Arthritis, Experimental , Arthritis, Rheumatoid/drug therapy , Cells, Cultured , Inflammation/pathology , Iridoid Glucosides , NF-kappa B/metabolism , X-Ray Microtomography
14.
Article in Chinese | WPRIM | ID: wpr-928326

ABSTRACT

OBJECTIVE@#To observe the effects of Taohong Siwu Decoction(, THSWD) on the mesenchymal stem cells(MSCs) migration, homing number and cytokine expression in callus during the early process of fracture healing, and to explore the mechanism of THSWD on accelerationg fracture healing by regulating the homing of MSCs in rats.@*METHODS@#A rat model of right femoral shaft open fracture was established. Thirty-two 5-week-old male Sprague-Dawley rats, weighting 110 to 130 g, were divided into control group, low-dose group, medium-dose group and high-dose group by using random number table. Distilled water was given to the control group, and the other groups were given Taohong Siwu Decoction. The rats were gavaged twice a day for 5 consecutive days after surgery. Bone volume/tissue volume(BV/TV) and bone mineral density(BMD) were observed using micro-computed tomography (micro-CT) at 21 days after surgery. At 5 days post-fracture, peripheral blood MSCs from THSWD treated and untreated rats were cultured in vitro. Subsequently, the migration ability of MSCs was observed by cell migration assay. The number of MSCs homing to the callus at the early stage of fracture (5 d) was detected by Immunohistochemistry (IHC). Protein chip was used to detect the expression of cytokines in callus.@*RESULTS@#Micro-CT results showed that BV/TV was higher in the high-dose group than in the medium-dose group (P=0.032), and higher in the medium-dose group than in the low-dose group(P=0.041), with no difference between the control and low-dose group (P=0.651). In addition, there was no difference in BMD between low-dose group and the model group (P=0.671), and lower in the low-dose group than in the medium-dose group(P=0.018), and the medium-dose group was lower than the high-dose group(P=0.008). Cell migration assay showed that THSWD promotes enhanced the migration ability of peripheral blood MSCs. IHC assay revealed that CD45-, CD90+, CD29+ MSCs significantly increased in bone callus after THSWD intervention compared with the control group. Protein chip showed that THSWD promoted the upregulation of CINC-1(×2.91), CINC-3(×1.59), LIX(×1.5), Thymus Chemokine (×2.55), VEGF (×1.22) and the down-regulation of TIMP-1 (×2.98).@*CONCLUSION@#THSWD, a representative formula of "promoting blood circulation and removing blood stasis", can significantly accelerate fracture healing, and its mechanism may be related to enhancing the migration ability of peripheral blood MSCs and up-regulating CINC-1, CINC-3, LIX, Thymus Chemokine, VEGF and down-regulating TIMP-1 in bone callus, which promotes the peripheral blood MSCs homing in the early stage of fracture.


Subject(s)
Animals , Humans , Male , Rats , Drugs, Chinese Herbal , Fracture Healing , Fractures, Bone/drug therapy , Mesenchymal Stem Cells , Rats, Sprague-Dawley , Tissue Inhibitor of Metalloproteinase-1/pharmacology , Vascular Endothelial Growth Factor A , X-Ray Microtomography
15.
Rev. odontol. UNESP (Online) ; 51: e20220045, 2022. ilus
Article in Portuguese | LILACS, BBO | ID: biblio-1424231

ABSTRACT

Introdução: o desafio no uso do Micro-Ct tem sido estabelecer e padronizar padrões adequados para escaneamento e tratamento das imagens, para que se obtenha o máximo desempenho do equipamento, e permitir a comparação dos achados entre diferentes estudos. Objetivo: o presente estudo tem como objetivo comparar a porcentagem de volume ósseo em regiões com perda óssea periodontal utilizando diferentes metodologias para definição da área a ser analisada (ROI). Material e método: dez ratos foram submetidos à indução de doença periodontal, e, após a eutanásia, as mandíbulas foram escaneadas com cortes de 9 µ e 18 µm de espessura, com passo de rotação de 0.3mm. As imagens foram reconstituídas utilizando o software NRecon, e em seguida, utilizando o software CTAnalyser - CTAn, foram definidas as áreas de interesse (ROI) ao redor dos segundos molares. A primeira área de interesse (ROI1) foi definida em um padrão retangular que se restringiu às regiões interproximais e furca, onde a área total de tecido ósseo foi obtida somando os resultados dos 3 ROIs. A segunda área (ROI2) foi definida no sentido corono/apical ao redor do segundo molar até suas limitações proximais com os dentes vizinhos, onde a porcentagem de tecido ósseo pertencente às raízes foram excluídas das avaliações. As análises foram realizadas com cinco diferentes valores de thresholds (130-50, 130-60, 130-70, 130-80, 130-90 e 130-100). Resultado: a análise entre os diferentes ROIs demonstrou que em ambas as análises foi observada a tendência a menor porcentagem de tecido ósseo quanto maior o contraste de tons de cinza utilizado, no entanto, nos resultados obtidos no ROI2 essa diferença não foi estatisticamente significante. Conclusão: com os resultados obtidos pode se concluir que a utilização de diferentes thresholds para quantificação óssea, em áreas onde houve doença periodontal, pode trazer resultados divergentes; a definição da área de interesse interfere com os resultados obtidos e que a obtenção de uma área de interesse com a remoção das raízes mostrou-se menos susceptível à variação dos parâmetros de escaneamento.


Introduction: the challenge in the use of Micro-Ct has been to establish and standardize adequate standards for scanning and image processing to obtain the maximum performance of the equipment and to allow the comparison of findings between different studies. Objective: this study aims to compare the percentage of bone volume in regions with periodontal bone loss using different methodologies to define the area to be analysed (ROI). Material and method: ten rats were submitted to periodontal disease induction, and, after euthanasia, the mandibles were scanned with slices of 9µ and 18 µm thickness, with a rotation step of 0.3mm. The images were reconstructed using the NRecon software, and then, using the CTAnalyser - CTAn software, the areas of interest (ROI) around the second molars were defined. The first area of interest (ROI1) was defined in a rectangular pattern that was restricted to the interproximal and furcation regions, where the total area of bone tissue was obtained by adding the results of the 3 ROIs. The second area (ROI2) was defined in the coronal/apical direction around the second molar to its proximal limitations with the neighboring teeth, in which the percentage of bone tissue belonging to the roots was excluded from the evaluations. Analyses were performed with five different threshold values (130-50, 130-60, 130-70, 130-80, 130-90, and 130-100). Result: the analysis between the different ROIs showed that, in both analyses, there was a tendency towards a lower percentage of bone tissue the greater the grayscale contrast used. However, in the results obtained in ROI2, this difference was not statistically significant. Conclusion: with the results obtained, it can be concluded that the use of different thresholds for bone quantification, in areas where there was a periodontal disease, can bring divergent results; the definition of the area of ​​interest interferes with the results obtained; and that obtaining an area of interest with the removal of the roots, proved to be less susceptible to the variation of the scanning parameters.


Subject(s)
Animals , Rats , Periodontal Diseases , Periodontitis , Alveolar Bone Loss , X-Ray Microtomography , Mandible , Molar , Analysis of Variance
16.
Rev. odontol. UNESP (Online) ; 51: e20220047, 2022. tab, ilus
Article in English | LILACS, BBO | ID: biblio-1424238

ABSTRACT

Introduction: Primary stability is one of the goals of modern implant dentistry and if achieved, reduces treatment time for prosthetic rehabilitation and the number of interventions made in patients mouth. Several companies state as protocol for connical conection implants, a subcrestally positioning. Objective: This in vitro study aimed to evaluate the effect of placing a conical connection implant equicrestally and subcrestally on static and loading condition in two types of bone density. Material and method: A total of 200 bone cylinders were extracted from femur of pigs, standardized by means of x-rays and computerized microtomography scan (microCT) and separated in low and high density specimens. The implants were placed on the center of the bone cylinders and were evaluated before and after loading by means of microCT and histomorphometry. Result: The results showed that placing the evaluated implant subcrestally provided better primary stability and performance on static and loading situations on low and high density bone. Conclusion: Placing implant subcrestally improve primary stability outcomes under loading and static situations.


Introdução: A estabilidade primária é um dos objetivos da implantodontia moderna e, caso atingida, reduz o tempo de tratamento para a reabilitação protéticas e o número de intervenções realizadas. Diversas empresas preconizam a posição subcrestal no uso de implantes com conexão cônica interna. Objetivo: Este estudo in vitro avaliou o efeito do posicionamento de implantes de conexão conica interna sub e equicrestal sob condições estáticas e em função, considerando dois tipos de densidades ósseas. Material e método: um total de 200 espécimes de osso extraído do femur de suínos e padronizados por meio de radiografias e microtomografias computadorizadas foram separados em densidade alta e baixa. Implantes foram instalados no centro dos especimes e for a avaliados por meio de microCT e histomorfometria. Resultado: Os resultados demonstraram que a colocação de implante subcrestalmente promoveu melhor estabilidade primária e performance em todas as situações, irrespectivamente à densidade óssea. Conclusão: A colocação de implantes subcrestalmente melhora a estabilidade primária em todas as situações, sendo indicada quando da utilização de conexões cônicas internas.


Subject(s)
In Vitro Techniques , Bone Density , Dental Implants , X-Ray Microtomography , Immediate Dental Implant Loading
17.
Int. j. morphol ; 40(5): 1261-1267, 2022. ilus
Article in English | LILACS | ID: biblio-1405287

ABSTRACT

SUMMARY: The objective of the study was to provide a detailed anatomical description of the rat's ear anatomy that will prove insightful to different experimental otologic surgical procedures regardless of scope. Three male Wistar rats were enrolled in the study. Candidates were screened for systemic and otologic pathology. External ear canal endoscopy was carried out with a 30˚ rigid endoscope through an image capture system. Middle ear anatomical elements were analyzed under stereomicroscopy. 3D computer tomography image reconstruction was realized with a micro-CT to describe the anatomy. Image data from all three rats were analyzed. Anatomical annotations and surgical exposure recommendations were added for key elements. The most relevant images from all three rats were selected for representation. Detailed visualization of the structural elements of the tympanic cavity were clearly visible: promontory, round window, stapedial artery, stapes, incus, and tympanic membrane were all constant findings. We describe a step wise ventral surgical approach of the middle and inner ear for which we found that the clavotrapezius muscle was a reliable landmark. For the transtympanic approach the endoscopic transcanal access was an easy and reliable method for which a detailed anatomical representation was depicted. Further, anatomical similarities to humans were observed by stereomicroscopy and Micro-CT imaging reiterating that the rat model is suitable for otologic research. The endoscopic approach to the tympanic membrane is comfortable and less expensive than a microscope. The tendon of the clavotrapezius muscle can be a reliable landmark for discovering the tympanic bulla when considering a ventral approach. 3D Micro-CT reconstruction allows intact evaluation of the samples, simultaneously being a diagnostic and also a learning tool.


RESUMEN: El objetivo de este trabajo fue proporcionar una descripción anatómica detallada de la anatomía del órgano vestíbulococlear de la rata que resultará útil para diferentes procedimientos quirúrgicos otológicos experimentales, independientemente del alcance. En el estudio se usaron tres ratas Wistar macho. Los ejemplares fueron evaluados por patología sistémica y otológica. La endoscopía del meato acústico externo se realizó con un endoscopio rígido de 30˚ a través de un sistema de captura de imágenes. Los elementos anatómicos del oído medio se analizaron bajo estereomicroscopía. La reconstrucción de la imagen de tomografía computarizada en 3D se realizó con un micro-CT para describir la anatomía. Se analizaron los datos de imagen de las tres ratas. Se agregaron anotaciones anatómicas y recomendaciones de exposición quirúrgica para elementos clave. Las imágenes más relevantes de las tres ratas fueron seleccionadas para su representación. La visualización detallada de los elementos estructurales de la cavidad timpánica era claramente visible: promontorio timpánico, ventana coclear, arteria estapedial, estapedio, yunque y membrana timpánica eran hallazgos constantes. Describimos un abordaje quirúrgico ventral escalonado del oído medio e interno para el cual encontramos que el músculo clavotrapecio era un punto de referencia confiable. Para el abordaje transtimpánico, el acceso transcanal endoscópico fue un método fácil y confiable para el cual se describió una representación anatómica detallada. Además, se observaron similitudes anatómicas con los humanos mediante estereomicroscopía e imágenes Micro-CT, lo que reitera que el modelo de rata es adecuado para la investigación otológica. El abordaje endoscópico de la membrana timpánica es cómodo y menos costoso que un microscopio. El tendón del músculo clavotrapecio puede ser un punto de referencia fiable para descubrir la bulla timpánica cuando se considera un abordaje ventral. La reconstrucción 3D Micro- CT permite la evaluación intacta de las muestras, siendo simultáneamente una herramienta de diagnóstico y también de aprendizaje.


Subject(s)
Animals , Male , Rats , Ear/anatomy & histology , Ear/surgery , Otologic Surgical Procedures , Rats, Wistar , Imaging, Three-Dimensional , Ear/diagnostic imaging , X-Ray Microtomography
18.
Pesqui. bras. odontopediatria clín. integr ; 22: e210112, 2022. tab, graf
Article in English | LILACS, BBO | ID: biblio-1386810

ABSTRACT

Abstract Objective: To evaluate the efficacy of silver diamine fluoride (SDF) in arresting dentin caries lesions when applied under different concentrations and times. Material and Methods: Forty-two bovine blocks were selected and fixed in 24-well plates. Each well received a mixed bacterial inoculum added to the culture medium with 5% sucrose. The plates were incubated in microaerophilia (7 days) for caries formation, confirmed by micro-CT (M1). SDF was applied over the carious lesions for different times and concentrations (n=6): SDF 30% - immediate removal, 1 minute and 3 minutes; SDF 38%, - immediate removal, 1 minute and 3 minutes. The group without treatment was the control. Then, the samples were again scanned by micro-CT (M2) and submitted to a second cariogenic challenge for 21 days. Then, a final scan was performed (M3). Results: Mean pH at the culture medium and lesion depth were compared using Kruskal-Wallis and Wilcoxon tests. 38% SDF showed the lowest metabolic activity of the biofilm. All 38% groups and 30% 1 and 3 minutes did not show an increase in mean lesion depth comparing M3 with M1. However, only 30% 3 minutes and 38% 1 and 3 minutes showed a significant reduction of lesion depth. Conclusion: The minimum application time of 30% SDF to arrest dentin caries lesion was 1 minute, while 38% SDF arrested with application and immediate removal.


Subject(s)
Animals , Cattle , Tooth Remineralization , Cariostatic Agents/therapeutic use , Dental Caries/epidemiology , Dentin , Diamines/chemistry , Fluorides/chemistry , Silver/therapeutic use , In Vitro Techniques/methods , Longitudinal Studies , Statistics, Nonparametric , Biofilms , X-Ray Microtomography/instrumentation
19.
Rev. Fac. Odontol. (B.Aires) ; 37(86): 1-12, 2022. ilus, tab
Article in Spanish | LILACS | ID: biblio-1413026

ABSTRACT

El objetivo de este estudio fue evaluar con microto-mografía los poros existentes entre el cemento de resina, poste de fibra y paredes del conducto en los distintos tercios radiculares en premolares inferio-res. Se utilizaron 15 premolares inferiores unirra-diculares humanos recientemente extraídos. Se les realizó el tratamiento endodóntico, y se obturó con conos de gutapercha y cemento endodóntico a base de resina. Una vez desobturados se procedió a la ce-mentación de los postes. Cada muestra se posicionó en un accesorio personalizado y se escaneó utilizan-do un Microtomógrafo. Con el software CTAn v.1.12 (Bruker-microCT) se analizaron las microtomografías para obtener el volumen de interés (VOI) que permi-tió calcular el área de superficie (mm2) y volumen de cada poro (mm3) entre la dentina y el poste a nivel co-ronal, medio y apical. Los datos fueron analizados me-diante las pruebas estadísticas de Friedman o ANOVA de medidas repetidas. El volumen de los poros entre los tres tercios radiculares mediante la prueba de Friedman, encontró una diferencia global significativa (F = 30,00; p < 0,05). El tercio en donde los poros presentaron un mayor volumen (mm3) fue el tercio coronal (mediana: 0,29250), seguido por los tercios medio (mediana: 0,03200), y apical (mediana: 0,00140). La comparación de la superficie de los poros entre los 3 tercios brindó un resultado análogo al de la comparación del volumen. La mayor superficie (mm2) correspondió al tercio coronal (media ± DE = 1,66377 ± 0,27175), seguido por los tercios medio (media ± DE = 1,16210 ± 0,20343) y apical (media ± DE = 0,41074 ± 0,12641). La microtomografía permitió realizar un análisis cuantitativo y cualitativo de los poros en toda la muestra, sin deterioro de la misma. Se puede concluir que el tercio coronal presenta más poros que el tercio apical con la técnica de cementación utilizada. En cuanto a la superficie y volumen de los poros, los resultados encontrados son similares a los reporta-dos por diversos autores (AU)


The aim of this study was to evaluate with microtomography the existing pores between the resin cement, fiber post and canal walls in the different in thirds of the canal, in single-root lower premolars. Fifteen recently extracted human single root lower premolars were used, endodontically treated, and filled with gutta-percha cones and resin-based endodontic cement. Once unfilled, the posts were cemented. Each sample was positioned on a custom fixture and scanned using a Microtomograph. Each sample was evaluated using CTAn v.1.12 software (Bruker-microCT) to obtain the surface area (mm2), volume of interest (mm3) of each pore between dentin and post at the coronal, middle and apical levels. Data were analyzed using Friedman's tests or repeated measures ANOVA. The volume of the pores between the three root thirds using the Friedman test, a significant global difference was found (F = 30.00; p < 0.05). The third in which the pores presented a greater volume (mm3) was the coronal third (means: 0.29250), followed by the middle (means: 0.03200) and apical (means: 0.00140) thirds. The comparison of the pore surface between the 3 thirds gave an analogous result to that of the volume comparison. The largest surface area (mm2) corresponded to the coronal third (mean ± SD 1.66377 ± 0.27175), followed by the middle (mean ± 1.16210 ± 0.20343) and apical (mean ± 0.41074 ± 0.12641) thirds.Microtomography allowed a quantitative and qualitative analysis of the pores in the entire sample without deterioration. It can be concluded that the coronal third has more pores than the apical third with the cementation technique used. Regarding the surface and volume of the pores, the results found are similar to those reported by various authors (AU)


Subject(s)
Porosity , Post and Core Technique , Cementation/instrumentation , X-Ray Microtomography , Bicuspid , Analysis of Variance , Resin Cements , Imaging, Three-Dimensional/methods , Fibric Acids
20.
Rio de Janeiro; s.n; 2022. 95 p.
Thesis in Portuguese | LILACS, BBO | ID: biblio-1554296

ABSTRACT

O conceito de Endodontia Minimamente Invasiva é amplo, e envolve não só a realização de acessos, mas também o uso de instrumentos com conicidades reduzidas ou geometrias inovadoras. Buscando avaliar se as estratégias minimamente invasivas adotadas apresentam vantagens frente aos tratamentos tradicionais, a presente tese foi dividida em três estudos. O estudo 1 avaliou a preservação da dentina perirradicular e o alargamento da porção apical dos canais de molares inferiores com instrumentos TruNatomy e ProTaper Gold. Para isso, vinte molares inferiores foram microtomografados, pareados e distribuídos em 2 grupos. No grupo ProTaper Gold, os canais mesial e distal foram preparados até os instrumentos F2 e F3, respectivamente, enquanto no grupo TruNatomy, os canais mesial e distal foram ampliados até os instrumentos prime e medium, respectivamente. Após um novo escaneamento, os parâmetros de instrumentação foram calculados. Os dados foram analisados pelos testes de Mann-Whitney, T Student e escalonamento multidimensional não-métrico. Não foram encontradas diferenças entre os grupos em relação à área não preparada e redução de espessura de dentina. O grupo ProTaper Gold removeu mais dentina que o TruNatomy no terço coronal das raízes mesiais. O estudo 2 avaliou a formação de microtrincas dentinárias em molares inferiores acessados de forma ultraconservadora (UltraAC), instrumentados com Reciproc e XP-Endo Shaper. Quarenta molares inferiores foram microtomografados, pareados e distribuídos em 4 grupos de acordo com o tipo de cavidade de acesso e protocolo do sistema de instrumentação: tradicional/Reciproc; tradicional/XP-endo Shaper; UltraAC/Reciproc e UltraAC/XP-endo Shaper. Após os preparos dos canais, os dentes foram escaneados novamente e as imagens transversais das raízes mesiais e distais, do nível da furca ao ápice foram avaliadas com o objetivo de identificar a presença de microtrincas dentinárias. Em todos os grupos, as microtrincas verificadas nas imagens pós-operatórias já estavam presentes no exame pré-operatório. O estudo 3 avaliou a influência de instrumentos com o mesmo diâmetro de ponta e diferentes conicidades no percentual de área não preparada e volume de dentina removida após o preparo dos canais mesiovestibular e distovestibular de molares superiores com ou sem o canal mesiovestibular 2 (MV2). Vinte e dois molares superiores foram selecionados, microtomografados, pareados e classificados em dois grupos, de acordo com a anatomia das raízes: dentes com MV2 e dentes sem MV2. Após o acesso, os canais radiculares foram preparados com instrumentos 25/.01, 25/.03, 25/.05, 25/.06 e 25/.08v. Os dentes foram submetidos a novos escaneamentos por micro-CT após preparo com cada instrumento descrito anteriormente. Os dados foram analisados usando o Modelo linear generalizado misto e aproximação de Kenward-Roger para testes Wald F. Em dentes com e sem canais MV2, a porcentagem de área não preparada apresentou uma diminuição significativa ao longo do tratamento após cada instrumento utilizado e a porcentagem de dentina removida apresentou um aumento significativo ao longo do tratamento. Com base nos estudos, concluiu-se que as estratégias minimamente invasivas adotadas não apresentaram vantagens frente aos tratamentos tradicionais(AU)


The concept of Minimally Invasive Endodontics is broad, and involves not only the realization of accesses, but also the use of instruments with reduced tapers or innovative geometries. Seeking to assess whether the minimally invasive strategies adopted have advantages over traditional treatments, this thesis was divided into three studies. Study 1 evaluated the preservation of periradicular dentin and enlargement of the apical portion of the mandibular molar canals prepared with TruNatomy and ProTaper Gold instruments. For this, twenty lower molars were microtomography, paired and divided into 2 groups. In the ProTaper Gold group, the mesial and distal canals were prepared up to the F2 and F3 instruments, respectively, while in the TruNatomy group, the mesial and distal canals were prepared up to the prime and medium instruments, respectively. After a new scan, the instrumentation parameters were calculated. Data were analyzed by Mann-Whitney, T Student and non-metric multidimensional scaling tests. No differences were found between groups regarding unprepared area and dentin thickness reduction. The ProTaper Gold group removed more dentin than TruNatomy in the coronal third of the mesial roots. Study 2 evaluated the formation of dentinal microcracks in mandibular molars accessed by ultraconservative cavites (UltraAC), instrumented with Reciproc and XP-Endo Shaper. Forty lower molars were microtomography, paired and divided into 4 groups according to the type of access cavity and instrumentation system protocol: traditional/Reciproc; traditional/XP-endo Shaper; UltraAC/Reciproc and UltraAC/XP-endo Shaper. After the root canal preparations, the teeth were scanned again and the transverse images of the mesial and distal roots, from the furcation level to the apex, were evaluated in order to identify the presence of dentinal microcracks. In all groups, the microcracks seen in the postoperative images were already present in the preoperative examination. Study 3 evaluated the influence of instruments with the same tip diameter and different tapers on the percentage of unprepared area and volume of dentin removed after preparation of the mesiobuccal and distobuccal canals of maxillary molars with or without the mesiobuccal canal 2 (MV2). Twenty-two maxillary molars were selected, microtomography, paired and classified into two groups, according to root anatomy: teeth with MV2 and teeth without MV2. After access, the root canals were prepared with instruments 25/.01, 25/.03, 25/.05, 25/.06 and 25/.08v. The teeth were rescanned by micro-CT after preparation with each instrument described above. Data were analyzed using the Generalized Linear Mixed Model and Kenward-Roger approximation for Wald F tests. In teeth with and without MV2 canals, the percentage of unprepared area showed a significant decrease throughout the treatment after each instrument used and the percentage of removed dentin showed a significant increase during treatment. Based on the studies, it was concluded that the minimally invasive strategies adopted did not present advantages over traditional treatments(AU)


Subject(s)
Humans , Male , Root Canal Preparation/methods , X-Ray Microtomography , Molar/diagnostic imaging , Root Canal Preparation/instrumentation , Dental Pulp Cavity
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