Histological response to platelet-rich plasma added to polypropylene mesh implemented in rabbits

ABSTRACT Introduction: The platelet-rich plasma (PRP) is part of a set of biotechnologies, providing some growth factors that promote repair of different tissues. The polypropylene meshes (PPM) are applied in the correction of abdominal defects, pelvic floor and urinary incontinence, however, they induce many significant complications, as a result of an inappropriate inflammatory response. Purpose: To investigate the changes caused by PRP associated with the implantation of PPM in the abdomen of female rabbits, in the production of collagen I and III and the inflammatory infiltrate (ININ). Materials and Methods: We performed implant meshes with and without PRP in adult rabbits (n=30) and euthanasia at 7, 30 and 90 days. Two plates were prepared from each animal and analyzed in five different fields. The ININ was evaluated by quantification of inflammatory cells using hematoxylin-eosin and the collagen by Sirius red method. The results were analyzed applying the Wilcoxon, Kruskal-Wallis, Junckheere and Friedmann tests. Results: There was a significant difference in the number of inflammatory cells between the groups with and without PRP (p=0.01) at 90 days. There was increased production of collagen I, III and total with the use of PRP, at seven days. Conclusion: The PPM coating with PRP was associated with increased ININ at the implant area, and an increasing trend during the process of tissue repair. The PPM coated with PRP was related to increased concentration of collagen I, collagen III and the concentration of total collagen increased after seven days of implantation.

Use of the international prostate symptom score in the assessment of severity of lower urinary tract symptoms in men / Uso do escore internacional dos sintomas prostáticos para avaliação da gravidade dos sintomas urinários em homens

Prevalence of urinary symptoms are very common among men over 40 years due to an increase in prostate volume known as benign prostatic hyperplasia (BPH). Several studies have shown a decline in quality of life for patients with severe lower urinary tract symptoms (LUTS). Objective: To investigate the severity of LUTS among men aging over 40 years concerning the feasible impairment in quality of life. Material and Methods: 117 men over 40 years who had attended at the urology outpatient unit were interviewed. The International Prostate Symptom Score (IPSS) was used to evaluate the severity of LUTS. Results: The most frequent complaint of the patients was the frequent waking at night with the urge to urinate (of these subjects 44,4% reported waking more than twice a night to urinate). 73,5% of the patients had mild symptoms, and 6% had severe symptoms. 37,2% of these subjects over the age of 61 years had moderate symptoms, and 14% had severe symptoms. 82,9% of patients was satisfied with their urinary condition. 54,2% of subjects having moderate symptoms and all subjects with severe symptoms reported some level of dissatisfaction. Conclusion: There is a close relation between the scores for urinary condition and patients? satisfaction. The International Prostate Symptom Score assess the severity of urinary symptoms and their impairment on quality of life as an important step toward an individualized treatment for patients.