Detection of Theileria infection in dromedary camels

Camels are important source of meat and wool. These products depend mainly on the general health conditions which are affected by infection with several diseases such as blood parasites. The examination was done on 215 camels by Giemsa stained blood films prepared from the blood collected from imported camels slaughtered at three slaughter house in Tripoli-Libya [February July 2008]. Out of 215 examined camels, 14 [6.5%] were infected with Theileria spp. The morphology of the detected Theileria in the erythrocytes was oval forms. The main size measured 1.3 x 0.4 micro m. The schizonts inside the lymphocyte were irregular in shape and showed varying number of red granules. Hyalomma dromedarii were identified from the camels infected with Theileria

Studies on intestinal helminthes infecting free-ranging red fox [vulpes vulpes] in north-western part of Libya

Eighteen free-ranging live red fox [Vulpes vulpes] captured during the period ringed from October 2007 to April 2008 from rural and semi-desert regions of North-Western part of Libya were investigated parasitologically for presenceof intestinal helminthes. The study revealed that 2 foxes [11.1%] were free from intestinal helminthes and 16 foxes [88.9%] were infected with helminth parasites species. These helminthes were four cestodes: joyeuxiella echinorhynocoides 5[31 .25%], mesocestoides spp. 6[37.5%], diplopylidium nolleri 2[1 2.5%] and dipylidium caninum 1[6.25%].Three nematodes: Rictularia caherrsis 3[1 8.7%], Ancylostomacaninum 3[18.7%] and Toxocara canis 2[12.S%].Only one acanthoeephala: moniliformis dubius 4[25%]. No trematodes were found. All these helminthes were recovered and reported for the first time from red fox [Vulpes vulpes] in north-western part of Libya. Five helminthes of them has zoonotic important. Therefore, the study showed that the red fox [Vulpes vulpes] plays an important role in the investigated area as a reservoir host of a number of parasites of domesticated and wild animals or may have zoonotic importance. The results were discussed with reference to variations in the live behavior and diet in the rural areas of North-western part of Libya

Characterization of trypanosome evansi antigen by SDS-page and western blot

SDS-PAGE of T.evansi antigen after staining with commassie blue stain showed the presence of 26 bands ranging between 222.56 - 9.0 KDa. Western blot analysis revealed immunoprecipitation only with 2 proteins components with camels positive serum and had molecular weight of 98.976 and 16.678 KDa. While with mice positive sera revealed only one immuoprecipitation with protein component had molecular weight 16.678 KDa

Incidence of theileriosis, babesiosis and anaplasmosis in cattle in Tripoli - Libya

Bovine theileriosis, babesiosis and anaplasmosis are a haemoparasitic disease caused by protozoans of Genus Theileria, Babesia and Anaplasma respectively. These haemoparasites are responsible for considerable losses due to mortality, weight loss and a reduction in milk beside the cost of prophylactic measures. The results showed that 51 [11.7%] and 13[2.9%] out of 443 cattle from different localities in Tripoli-Libya by Giemsa stained blood and lymph node smears were infected only with Theileria species respectively. The incidence of Theileria species by Giemsa stained blood films examination in different localities in Tripoli was 0.0%, 22.7% and 35.4% in Engeela, Algyran and Fum-Mulgha respectively. While the total incidence of T.mutans, B.bigemina and A.marginale in the examined cattle by different ELISA kits were 50.6%, 12.9, and 3.4%, respectively. Also, the present work recorded mixed infection

Detection of Trypanosoma evansi in dromedary camels in Libya

The infection of camels with trypanosomosis in Tripoli-Libya was conducted for the first time by this work. Antibodies against T.evansi was detected in 13 [6.2%] of 210 camels serum samples using CATT at dilution 1: 4 and 10 [4.8%] of 210 camels serum samples by ELISA at dilution 1: 25. T.evansi was not detected in 210 camels Giemsa stained blood films examination

Prevalence of toxoplasma gondii infection in wild animals in north-western part of Libya

The infection of wild animals with toxoplasmosis in Libya was conducted for the first time by this work. Antibodies to Toxoplasma gondii were detected in samples collected from 45 wild animals [free-ranging live] and captured at North-Western part of Libya. Serum was tested using Latex agglutination tes t[LAT]. 4 out of 30 hedgehogs, 2 out of 10 red fox [Vulpes vulpes] and 1 out of 5 porcupines serum samples tested positive. The titers were 1/40, 1/80, 1/160 and 1/320 in hedgehogs, 1/80 and 1/160 in red fox but in porcupines was 1.80