[ inhibitory effect of Artemisia annua extracts on gastric cancer cells via apoptosis induction]

Previous studies have shown an anti-tumoral effect for different species of Artemisia. This study was performed to evaluate the anti-tumural effects of different kinds of Artemisia annua extracts on gastric cancer cells. Methanol, ethylacetate, dichloromethane and hexane extracts of Artemisia annua were prepared by step to step procedure. Cultivated gastric cancer cell line [AGS] and normal fibroblast cells [L929] were incubated with different concentrations of extracts for 24 hours and cell growth inhibition was determined using MTT assay and results were reported as lC50 [concentration that caused 50 percent inhibition of cells growth]. Annexin V and propidium iodide [PI] staining was used to evaluate the apoptosis and/or necrosis induction in cancer cells. One way ANOVA was used for analysis. The results obtained from MTT assay showed a strong and dose-dependent inhibition of cancer cell growth by different Artemisia annua extracts. The most cytotoxic effect was obtained by methanolic extract [IC50: 500 microg/ml] and it caused apoptosis more than other extracts. Isolated extracts from Artemisia annua caused a significant decrease in gastric cancer cell growth mainly by induction of apoptosis and at the same time they had less toxicity on normal cells. Therefore, Isolation and purification of effective compound/s from this extracts and determination of their mechanisms of action is suggested

[Study on toxic effects of Artemisisa spp. fractions from Iran on human cancer cell lines]

For most of cancers there is no treatment and most of them ended in death. So, the first investigational stage is evaluation of toxic effects of drug fractions on cancer cells. Artemisia species are important medicinal plants throughout the world. In this study, anti-tumoral effects of seven Artemisia spp. fractions from Iran were studied on cancer and normal cells. Ethanol, ethylacetate, dichloromethane and hexane fractions of seven Artemisia incubated with different concentrations of fractions for 72 hours and cytotoxicity was determined using MTT assay. Results were reported as IC[50] [concentration that kills 50 percent of cells]. Obtained results showed strong and dose-dependent inhibition of cancer cell growth by different Artemisia fractions. The most cytotoxicity effects were for dichloromethane fraction from Artemisia biennis on cervix cancer cells, dichloromethane fraction from Artemisia ciniformis on gastric cancer cells and dichloromethane fraction from Artemisia diffusa on colon cancer cells. Ethylacetate, dichloromethane and hexane fractions from Artemisia biennis, hexane fraction from Artemisia ciniformis, hexan fraction from Artemisia santulina and ethylacetate fraction from Artemisia vulgaris had the least toxic effect on normal L929 cells. Some isolated fractions caused a significant decrease in cancer cell growth and had less toxicity on normal cells. So, study on Artemisia in prevention or efficient treatment of different cancers is useful. Study the effect of effective fractions on apoptosis induction and determination of their mechanisms of actions is suggested

[Studying the volume and weight of the brain index in boy between the ages of 18-22 in the city of Tehran year 1385]

Background: cephalometry discusses different measures of the head and face and having this information is very important in foreseeing the scale of growth of the skull. By having the dimensions of the skull we are able to calculate the weight, volume and index of the brain. Acknowledging the above mentioned information can be helpful for the evaluation of health of sickness in infants, youth and in the elderly

Materials and Methods: this research was done under the cross-sectional method. 560 Samples of boys between the ages of 18-20 in the city of Tehran were studied. All were sane and none of them contained any effective disease. The weight was measured with the minimum amount of clothes and by German scale called Sohnel with 0/5 mm accuracy, the length and width of the face was measured by the Martinsaler scaled cephalometre weight was measured with 0/5 Kg accuracy and height by stadiometer with 1 mm accuracy. Then the weight, volume and index of the brain were measured

Results: the mean of the length of the head in boys between the ages of 18-22 year olds were as follows: 188/7 + 7/7 and 188/6 +8/7 millimeter, Width: 152/7 + 6/5 and 156 + 6/9 millimeter, Height: 69/7 + 11/5 and 70/8 + 10/5, Auricular Height: 132/1 + 7/1 and 133/4 + 8/1 millimeters, Brain Volume: 1411/4 + 76/8 and 1438/5 + 11, Brain Weight: 1460/8 + 82/6 and 1488/8 + 114/9, Brain Index: 2/1 + 0/3 and 2/1 +]/3

Conclusion: the length of the head in between the ages of 18-22 has not changed but the weight, auricular height, volume and weight of the brain have ascended. However the brain index has been the same in all ages [except for 19 year olds]

[ Study of appendix position in patient with previously appendectomy in Kerman medical centers in 2003-2005]

Background: identifying the exact position of appendix and completion of its anatomic information has great importance in both of basic aspect and clinical value. Whereas, acute appendicitis is the most common and emergency in the surgical abdomen, the identification of the variations of the appendix position can be very useful to surgeons

Materials and Methods: in a cross sectional retrospective study, 1041 files of patients with previously appendectomy in the Kerman medical centers in 2003-2005, were studied

Results: the number of patients which they were under appendectomy was 336[32.3%] males and 705[67.7%] females that their range of ages was between of 12 to 70 years old. The analysis of the data is indicated: in the males, 325[96.7%] patients have posterior position appendix and 11 [3.3%] patients have anterior position appendix. In females, 684[97%] patients and 21[3%] patients have posterior position and anterior position appendix, respectively. The most common posterior position in both genders was the subcaecal which was observed 81.5% in males and 84.9% in females. The rarest posterior position in both genders was the retroileal which was observed 0.6% males and 0.1% females. The most common anterior position in both genders was the preileal which was observed 45.4% in males and 57.1% in females. The rarest anterior position in males was the pararectal which was observed 9.1% and in females was the precaecal which was observed 4.8%

Conclusions: in the posterior position, the most type of appendix was the subcaecal and the least type of appendix was the retroileal and in the anterior position, the most type of appendix was the preileal, in both genders. But, in the anterior position, the pararectal in males and the precaecal in females were the the least type of appendix

[Studying the classic norm of the face of boys between the ages of 18-22 based on the prosopic index in Tehran in 2005]

Background: cephalometry discusses the characteristics on the measures of different soft and hard tissues of the head and face and it is considered an important Para-clinical record for the growth of the index and also for the diagnosis and curing orthodontic matters. Studying the face characteristics is very useful in sciences such as dentistry, medical, forensic and industry. Therefore, the objective of the following research is to study the categorization of the face types of boys between the ages of 18-22 in Tehran on 2005

Materials and methods: this research was done according to the descriptive method by observation, interviewing and measurement. 662 samples of boys between the ages of 18-22 were measured by Cephalometric standards according to the classic method. Samples were healthy and none of them contained any effective diseases. The most important dimensions of the face are its length and width which were measured by Martin Saller Cephalometer with 0.5 mm accuracy

Results: the face length between the ages of 18-22 was 124 +/- 9.2 and its width was 138 +/- 9.1 and the face index was 89.2 +/- 5.2

Conclusions: according to the resulting dimensions, the face was categorized [vicro cranium] in persons. In all ages the hyperauriprosopic were the rare type and the mesoprosopic were the dominant type