RESUMEN
Background and Objective: Pseudomonas aeruginosa is an opportunistic pathogen with numerous virulence factors such as phospholipase and type IV pili. The emergence of multidrug resistant Pseudomonas aeruginosa has become a serious public health threat worldwide. This study was done to determine the frequency of plcH, plcN, pilA and pilB genes in multi-drug resistant Pseudomonas aeruginosa isolated from clinical samples
Methods: In this cross-sectional study, 93 isolates of Pseudomonas aeruginosa collected from different clinical samples from hospitals of Zanjan, Iran during 2013-14. After identification of isolates by biochemical tests, antibiotic susceptibility testing [Kirby-Bauer] was performed according to CLSI guidelines. Total DNA extracted and PCR was done to detect of plcH, plcN, pilA and pilB genes
Results: Among 93 of Pseudomonas aeruginosa isolates, the highest antibiotic resistance related to Erythromycin and Cefoxitin [95.6%] and the lowest resistance related to Amikacin [26.8%]. 80.6% of isolates were multidrug resistant [MDR]. Out of 75 MDR isolates, the frequency of plcH, plcN, pilA and pilB genes was 97.4%, 49.3%, 26.6% and 17.3%, respectively
Conclusion: According to high frequency of phospholipase C gene [plcH] in MDR Pseudomonas aeruginosa isolates which isolated from different clinical samples, presumably this virulence factor plays an important role in pathogenesis of this bacterium