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Journal of Gorgan University of Medical Sciences. 2017; 19 (2): 91-97
en Persa | IMEMR | ID: emr-189304

RESUMEN

Background and Objective: Pseudomonas aeruginosa is an opportunistic pathogen with numerous virulence factors such as phospholipase and type IV pili. The emergence of multidrug resistant Pseudomonas aeruginosa has become a serious public health threat worldwide. This study was done to determine the frequency of plcH, plcN, pilA and pilB genes in multi-drug resistant Pseudomonas aeruginosa isolated from clinical samples


Methods: In this cross-sectional study, 93 isolates of Pseudomonas aeruginosa collected from different clinical samples from hospitals of Zanjan, Iran during 2013-14. After identification of isolates by biochemical tests, antibiotic susceptibility testing [Kirby-Bauer] was performed according to CLSI guidelines. Total DNA extracted and PCR was done to detect of plcH, plcN, pilA and pilB genes


Results: Among 93 of Pseudomonas aeruginosa isolates, the highest antibiotic resistance related to Erythromycin and Cefoxitin [95.6%] and the lowest resistance related to Amikacin [26.8%]. 80.6% of isolates were multidrug resistant [MDR]. Out of 75 MDR isolates, the frequency of plcH, plcN, pilA and pilB genes was 97.4%, 49.3%, 26.6% and 17.3%, respectively


Conclusion: According to high frequency of phospholipase C gene [plcH] in MDR Pseudomonas aeruginosa isolates which isolated from different clinical samples, presumably this virulence factor plays an important role in pathogenesis of this bacterium


Asunto(s)
Resistencia a Múltiples Medicamentos , Fosfolipasas , Proteínas Bacterianas , Genes
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