[Molecular diagnosis of B cell Non-Hodgkin Lymphoma by evaluation of immunoglobulin heavy chain gene rearrangement]

Rearrangement of V, D, and J segments of immunoglobulin heavy chain gene with inserted or deleted nucleotides within rearranged segments makes unique hypervariable regions [CDR-3]. These regions can be used for evaluation of B cell clonality for the purpose of molecular diagnosis of Non-Hodgkin Lymphoma [NHL] and for confirmatory diagnosis in suspicious cases. In this study, samples of 42 patients were collected from Taleghani, Baqhiyatalah, and Aliasghar hospitals; out of this number, there were 22 patients with diagnosis of B cell NHL, 10 with reactive hyperplasia, and 10 with malignant lymphoma. After DNA extraction from formalin fixed paraffin embedded tissues, PCR was done using consensus primers for amplification of CDR-3 region. PCR products were analyzed after heteroduplex analysis using polyacrylamide gel electrophoresis and silver stain. Results Clonal patterns in group 1 [B cell NHL], 2 [reactive and follicular hyperplasia], and 3 [morphological diagnosis without immunohistochemistry] were observed in 77.2%, 0%, and 70% of patients, respectively. Our findings are compatible with other international studies with minor differences. The diagnosis of B-cell lymphoid malignancy can frequently be substantiated by detecting clonal immunoglobulin heavy chain [IGH] gene rearrangement

[Clinical and microscopic findings In 71 cases of craniopharyngioma referred to Tehran Shariati Hospital]

Craniopharyngiomas make up about 1-3 percent of primary tumors of the CNS. This tumor can develop at any time from birth to old age, but more than 50% of patients are younger than 20 years. Most of them are suprasellar, but nearly 25% have an intrasellar component. Most of the clinical manifestation of tumor are related to their compressive effects, and include headache, visual disturbance, hydrocephalus and endocrine abnormalities. Two distinct variants of craniopharyngioma are recognized, the classic adamantinomatous type and a recently described papillary form. In this study we describe clinicopathologic findings of 71 patients diagnosed with craniophayngioma. The majority of patients were male, patients age ranged between 4 to 51 years and headache was the commonest presenting symptom. Microscopically most of them were adamantinomatous type and only 5 cases were papillary type. The most common post operative complication was recurrence of disease, and all of them were adamantinomatous type