Serum levels of p53, bcl-2 and lipids during experimental breast carcinogenesis and the protective effect of some natural and chemical agents

To investigate the possible protective effects of natural agents Nigella sativa [NS], Soybean [SB] and Vitamin C [Vit.C.] as well as chemical agent Aspirin [Asp.] against breast carcinogenesis induced in experimental animals using 9,10 Dimethyl benz [a] 1,2 anthracine [DMBA] as a potent breast.carcinogen. Materials and A single dose of dimethylbenz[a]anthracene [DMBA, 15mg], was administered to rats via intragastric intubation. The animals were divided into six groups [control, DMBA, [DMBA + Aspirin], [DMBA + Vitamin C], [DMBA + Nigella sativa] and [DMBA + Soybean] group]. Biochemical and histopathological analyses were done on serum and mammary glands tissue. The level of serum p53 and bcl-2 proteins was measured by ELISA and western blot techniques. The level of serum triacylglycerol [TG], cholesterol, high density lipoprotein [HDL] and low density lipoprotein [LDL] were determined spectrophotolmetrically. The protective effect of Asp, Vit.C, NS and SB was demonstrated by reducing the incidence of glandular tumors induced by DMBA from 73.3% to 50%, 43.3%, 40% and 36.6%, respectively. These protective agents, reduced also the levels of p53, bcl-2, TG and LDL, and attenuated the effect on HDL level. DMBA induced mutant p53 and bcl-2 over-expression were associated with early onset of carcinogenesis. Asp., NS, SB and Vit. C exhibited chemoprotective effect against tumor induction, through their antimutagenic effect

Cytogenetic studies of chemoprophylactic agents on postimplantion mice embryo in presence of nitrosamine: effect of soybean

Nitrates, nitrites and dimethylamine are a components reacts in the gastro-intenstinal tract synthesing the powerful hepatoxic and carcinogenic dimethylnitrosmamine. This study aimed to evaluate the cancer protector named soybean. This evaluation had been tested in laboratory mice in the presence of nitrosamine as a cancer induced. For pursing the evaluation, four groups of mothers and their embryos were used. The first group was the control group, the second group was a mothers treated with Dibutylamine plus nitrite [DBA + NO3], the third group was a mothers fed with a diet containing a 30% soybean, while the fourth group was mothers fed with SB and DBA + NO3. Generally the results showed a decrease in the percentage of gestation in all the treated animals in comparison with the control group, while there was no significant difference [P>0.05] in the mean numbers of impanation sites and living fetuses in all the treated animals. Moreover the cytogenetical analysis revealed that soybean produced a non significant ratio of chromosome abnormalities when tested using the Chi-square method