RESUMEN
The present study investigated the possibility of replacing salamon with modified shotor diluent [MSD] and egg yolk [EY] with low density lipoprotein [LDL] for chilled storage of ram semen. Good quality semen [>80% progressive forward motility [PFM] of sperm] from 3 fertile rams was collected using an artificial vaginaandpooled for each experiment. Low density lipoprotein was extracted from fresh EY. In experiment 1, semen was divided into 2 fractions and extended in MSD or salamon. In experiment 2, semen was assigned into 5 fractions and extended in MSD supplemented with 12 and 15% EY or 3, 5 and 8% LDL. In experiment 3, semen was divided into 2 fractions and extended in MSD supplemented with 12% EY or 5% LDL. Viability of sperm was assessed at times 0 [immediately after semen dilution], 2 or 4 [at 4°C] and up to 72 h after semen dilution. Data was analyzed using General Linear Model [GLM] procedure, including repeated measures. In experiment 1, the viability of sperm was similar in two diluents [P>0.05]. In experiment 2, PFM of sperm was similar among groups at the time of dilution [P>0.05]; but remained elevated in 5 and 8% LDL compared to other groups afterward [P<0.05]. In experiment 3, PFM of sperm was superior at 48 and 72 h after dilution in 5% LDL compared to 12% EY [P<0.05]. In conclusion, MSD supplemented with 5% LDL is a suitable diluent for ram fresh semen preserved at 4°C for 72 h. Key words: Ram, Semen preservation, Low density lipoprotein, Diluent
RESUMEN
To present a more accurate method for diagnosis of Cysticercus bovis in slaughtered cattle. ProjectrSlaughterhouse study. Animal:3436 cattle.To present a more accurate method for diagnosis of C.bovis in slaughtered cattle 1812 cattle were examined at meat inspection using traditional method. Meanwhile by using standard method for detection of c.bovis at meat nspection in South Africa,Germany and England 145 cattle for each region were examined.In each method the percentage of infection with C.bovis was determined. In raditional method heart,external and internal master and ;riceps muscles were inspected.Whereas for the method used in Germany and England,heart,external and internal master,oesophagus,diaphragm, tongue and for that of South Africa in addition to these,triceps was also inspected. The best, among the method used, was selected and according to the latter 1189 carcasses were examined. If the accuracy of selected method was statistically superior or similar to the best method,thus it was considered as an alternative or replacement method to the traditional method.By examining of 1182 cattle at meat inspection using traditional method 47 carcasses [2.6%] harboured .bovis in different organs,whereas using standard method,used in Germany,England and South Africa 7.6%, 6.9% and 6.9% of examined cattle were found to be infected with' C.bovis.There was a significant differences between traditional method and standard methods of three different countries us in X2 test. Standard method used in Germany was more accurate than the others, thus it was chosen as our selected method by which 1189 cattle were examined of which ninty cattle [7.6%] were found to be infected with C.bovis, an infection rate comparable to German method, Thus it could be suggested as an alternative or replacement to the traditional method