Study of some gut hormones in different gall bladder disorders in diabetics and non-diabetic patients

In a trial to explore the aetiopathogensis of gall bladder disorder in diabetics, two hormones [somatostatin and gastrin] with opposed actions on gall bladder [GB] in motility were assessed. Fifty eight subjects were included 36 diabetics [G[1]] [12 with normal GB [G[1A]], 12 with biliary dyskinesia [G[1B]], 12 with gall stone disease [G[1C]] 12 non diabetic with gall stone disease [G[2]], 10 healthy controls [G[3]]. They were subjected to clinical study, routine laboratory investigations, measurement of glycosylated haemoglobin and fasting serum gastrin, plasma somatostatin, sonographic assessment of GB kinetics and morphology, body mass index [BMI] determination. Serum gastrin level was significantly elevated among the studied groups compared to those of controls with the highest value in G[1C]. Plasma somatostatin level was significantly elevated in diabetics insignificantly did so in non-diabetics with gall stone disease Glycosylated haemoglobin was significantly elevated in diabetics [G[1B] > G[1C] > G[1A]]. Diabetics, non diabetics with GB disorders showed a significant reduction in GB ejection fraction. [G[1C] < G[1B] < G[2]] and a significant elevation in BMI[G[1C] = G[1B] > G[2]]. We concluded that the elevated level of the inhibitory hormone somatostatin in diabetics might participate in the pathogenesis of GB disorders. Elevated level of the weak GB contractility stimulant hormone gastrin seemed to be an ineffective compensatory response

Reactions of di-and tri-hydric phenolic drugs with trioxalato ferrate [III] reagent and their analysis in aqueous media

Simple rapid and reliable spectrophotometric procedures were adopted for microdetermination of catechol, dopamine hydrochloride [drug] and pyrogallol using trioxalatoferrate [III] complex [A] as a coloring reagent via formation of colored mixed ligand chelates with these phenolic drugs. A series of experiments were performed covering a wide range of concentration of the abovementioned drugs in the suitable conditions. The standard deviation was calculated and found to be 0.1 to 1.22. The obtained results indicated the successful application of complex [A] as selective indicator for microdetermination of dopamine in its pharmaceutical ampules and total catecholamines in urine samples of some tumor Egyptian patients

Effect of surgery on IgG secreting B cells in peripheral blood

IgG was assayed in 27 patients admitted to elective surgery. Blood samples were taken before, 4, 7 and 21 days after operation. IgG was barely detectable before and 4 days after operation, showing a marked increase at the 7th day and disappeared again at 21st day. These events suggested that activated B cells secreting IgG represent a sensitive marker of recent antigenic exposure and showed the positive effect of surgery on antibody secretion