Chemical investigation and anti-complement activity of chrysanthemum morifolium ramer

The 80% EtOH extract of aerial parts [leaves and stem] of Chrysanthemum morifolium showed a significant anti-complement activity [IC[50]= 5.65 micro g/ml]. Bioassay guided fractionation of the active extract has led to the isolation of a coumarin [scopoletin], orthocoumarinic acid 8-glucoside, melilotoside, 3,5- dicaffeoylquinic acid and the numbing principle [N-isobutyl-2-trans-4-trans-5-[alpha-thienyl]- decadienamide]. The structures of these compounds were elucidated by means of TLC, FAB-MS and 1D and 2 D NMR spectroscopy