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1.
Electron. j. biotechnol ; 35: 25-32, sept. 2018. graf, ilus
Artículo en Inglés | LILACS | ID: biblio-1047765

RESUMEN

Background: Pollen development is an important reproductive process that directly affects pollen fertility and grain yield in rice. Argonaute (AGO) proteins, the core effectors of RNA-mediated silencing, play important roles in regulating plant growth and development. However, few AGO proteins in rice were reported to be involved in pollen development. In this study, artificial microRNA technology was used to assess the function of OsAGO17 in pollen development. Results: In this study, OsAGO17, a rice-specific gene, was specifically expressed in rice pollen grains, with the highest expression in uninucleate microspores. Downregulation of OsAGO17 by artificial microRNA technology based on the endogenous osa-miRNA319a precursor was successfully achieved. It is found that downregulation of OsAGO17 could significantly affect pollen fertility and cause pollen abortion, thus suggesting that OsAGO17 functions in rice pollen development. In addition, the downregulation of OsAGO17 mainly caused a low seed-setting rate, thereby resulting in the reduction of grain yield, whereas the downregulation of OsAGO17 did not significantly affect rice vegetative growth and other agricultural traits including number of florets per panicle, number of primary branch per panicle, and 100-grain weight. Furthermore, the result of subcellular localization analysis indicated that the OsAGO17 protein was localized to both the nucleus and the cytoplasm. Conclusion: These results represent the first report of the biological function for OsAGO17 in rice and indicate that OsAGO17 may possibly play crucial regulatory roles in rice pollen development. It helps us to better understand the mechanism of pollen development in rice.


Asunto(s)
Polen/crecimiento & desarrollo , Oryza/crecimiento & desarrollo , Regulación hacia Abajo , Proteínas Argonautas/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , MicroARNs , Interferencia de ARN , Fertilidad , Proteínas Argonautas/genética
2.
Journal of Huazhong University of Science and Technology (Medical Sciences) ; (6): 938-942, 2017.
Artículo en Chino | WPRIM | ID: wpr-333400

RESUMEN

The clinical application of orbital magnetic resonance (MR) T2-mapping imaging in detecting the disease activity of Graves' ophthalmopathy (GO),and the predictive values of therapy response to intravenous glucocorticoid (ivGC) were investigated.Approved by the local institutional review board (IRB),106 consecutive patients with GO were included in this prospective study.All subjects were divided into two groups according to the patients' clinical activity score (CAS):the CAS positive group (CAS ≥3) or the CAS negative group (CAS <3).T2 relaxation time of extraocular muscles (T2RT;ms) and the areas of four extra-ocular muscles (AEOMs;mm2) were measured by 3D T2-mapping MR sequence before and after methylprednisolone treatment,so as the CAS and some ophthalmic examinations including visual acuity,intra-ocular pressure,eyeball movement,diplopia and proptosis.In addition,24 healthy volunteers were recruited as the control group.The mean T2RT and AEOMs in CAS positive group were higher than those in CAS negative group.Both CAS positive and negative groups had significantly higher mean T2RT and AEOMs than the control group (P<0.01).There was a positive correlation between T2RT and AEOMs values in GO patients,both of them had a positive correlation with CAS and the ophthalmic examinations.It was concluded that to evaluate the activity of GO,CAS was mostly related to inflammation symptoms of ocular surface,more than that,T2RT and AEOMs were also related to abnormal findings of the ophthalmic examinations including high ocular pressure,impaired eyeball movement,diplopia and proptosis.T2RT and AEOMs can reflex the inflammation state of ocular muscles better.CAS combined with 3D T2-mapping MR imaging could improve the sensitivity of detection of active GO so as the prediction and evaluation of the response to methylprednisolone treatment.

3.
Chinese Journal of Pathology ; (12): 309-313, 2012.
Artículo en Chino | WPRIM | ID: wpr-241924

RESUMEN

<p><b>OBJECTIVE</b>To explore prognostic factors and the expression of glypican-3, hepatocyte antigen (HEP), alpha-fetoprotein (AFP), CD34 and CD10 in hepatocellular carcinoma (HCC) and their prognostic value.</p><p><b>METHODS</b>Clinicopathologic data were analyzed in 375 cases of HCC, in which 80 cases with follow-up were examined by immunohistochemical staining to detect the expression of glypican-3, HEP, AFP, CD34 and CD10 proteins. The relationship between the proteins expression and clinicopathologic features was also evaluated.</p><p><b>RESULTS</b>Tumor number (P = 0.000), tumor size (P = 0.025), tumor differentiation (P = 0.001) and vessel invasion (P = 0.000) were closely related to prognosis of HCC patients; the expression of glypican-3 (66/80,82.5%; P = 0.002), HEP (64/80,80.0%; P = 0.021), AFP (38/80,47.5%; P = 0.014) and CD10 (28/80,35.0%; P = 0.002) was significantly related to tumor differentiation; that of glypican-3 was significantly correlated with tumor number and presence of satellite nodules (P = 0.028) and that of AFP and CD10 was significantly correlated with portal vein thrombi (P = 0.000, P = 0.010). On Kaplan-Meier regression analysis, both low expression of HEP and high expression of AFP were closely related to poor prognosis.</p><p><b>CONCLUSIONS</b>Tumor number, size, differentiation and vessel invasion were important factors affecting the prognosis of patients with HCC. HEP and AFP have prognostic significance in HCC.</p>


Asunto(s)
Femenino , Humanos , Masculino , Antígenos , Metabolismo , Antígenos CD34 , Metabolismo , Biomarcadores de Tumor , Metabolismo , Carcinoma Hepatocelular , Metabolismo , Patología , Cirugía General , Diferenciación Celular , Estudios de Seguimiento , Glipicanos , Metabolismo , Hepatocitos , Alergia e Inmunología , Neoplasias Hepáticas , Metabolismo , Patología , Cirugía General , Neprilisina , Metabolismo , Vena Porta , Patología , Pronóstico , Tasa de Supervivencia , Carga Tumoral , Trombosis de la Vena , Patología , alfa-Fetoproteínas , Metabolismo
4.
Chinese Journal of Pathology ; (12): 747-751, 2012.
Artículo en Chino | WPRIM | ID: wpr-256301

RESUMEN

<p><b>OBJECTIVE</b>To study the clinical and pathologic characteristics of small cell neuroendocrine carcinoma of urinary tract.</p><p><b>METHODS</b>All cases of urinary tract carcinoma encountered in the General Hospital of People Liberation Army during the period from 1999 to 2010 were retrospectively reviewed. The clinicopathologic data of small cell neuroendocrine carcinomas were further analyzed, with literature review.</p><p><b>RESULTS</b>A total of 16 cases of small cell neuroendocrine carcinoma were identified, including 10 from urinary bladder, 2 from ureter, 3 from renal pelvis, and 1 multifocal tumor involving renal pelvis and ureter. There were altogether 8 males and 8 females. The median age of the patients was 63 years (range = 24 to 79 years). Gross hematuria (11 cases) represented the main presenting symptom. Four patients had flank pain and 4 had urinary irritation symptoms. Seven patients underwent radical cystectomy. Six other patients underwent radical nephroureterectomy, 1 partial cystectomy, 1 TURBT and the remaining case biopsy only. The size of the tumor ranged from 0.8 to 8.0 cm (median = 4.5 cm). Histologically, 15 cases represented mixed small cell neuroendocrine carcinoma (with 13 mixed with transitional cell carcinoma and 2 with adenocarcinoma). Immunohistochemical study showed positive staining for neuroendocrine markers. On presentation, 1 patient was in stage pT1, 7 in stage pT2, 6 in stage pT3, 2 in stage pT4. Six patients died of the disease after operation. The overall survival was 25 months and the 5-year survival rate was 32.4%.</p><p><b>CONCLUSIONS</b>Small cell neuroendocrine carcinoma of urinary bladder is a highly malignant disease and associated with poor prognosis. The diagnosis relies on detailed histologic examination. Early diagnosis, when coupled with cystectomy or nephroureterectomy and adjuvant chemotherapy, represents the mainstay of management.</p>


Asunto(s)
Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Adulto Joven , Antígeno CD56 , Metabolismo , Carcinoma Neuroendocrino , Quimioterapia , Metabolismo , Patología , Cirugía General , Carcinoma de Células Pequeñas , Quimioterapia , Metabolismo , Patología , Cirugía General , Quimioterapia Adyuvante , Cistectomía , Estudios de Seguimiento , Queratinas , Metabolismo , Neoplasias Renales , Quimioterapia , Metabolismo , Patología , Cirugía General , Estadificación de Neoplasias , Nefrectomía , Fosfopiruvato Hidratasa , Metabolismo , Estudios Retrospectivos , Tasa de Supervivencia , Sinaptofisina , Metabolismo , Neoplasias Ureterales , Quimioterapia , Metabolismo , Patología , Cirugía General , Neoplasias de la Vejiga Urinaria , Quimioterapia , Metabolismo , Patología , Cirugía General , Neoplasias Urológicas , Quimioterapia , Metabolismo , Patología , Cirugía General
5.
Chinese Journal of Pathology ; (12): 538-542, 2012.
Artículo en Chino | WPRIM | ID: wpr-303528

RESUMEN

<p><b>OBJECTIVE</b>To study the clinicopathologic features and histologic differential diagnosis of small cell neuroendocrine carcinoma (SmCC) of kidney.</p><p><b>METHODS</b>The clinicopathologic features of 12 cases of SmCC of kidney encountered during the period from 1999 to 2010 were retrospectively reviewed.</p><p><b>RESULTS</b>Six cases of primary and 6 cases of metastatic SmCC involving kidney were identified. Amongst the primary renal SmCC, 2 were located in renal parenchyma and 4 in renal pelvis. Chest X-ray showed negative findings. Five of them underwent radical nephrectomy. On gross examination, the tumor was located centrally around the renal pelvis in 4 cases and peripherally in renal parenchyma in 1 case. On the other hand, 4 of the 6 cases of metastatic SmCC were discovered during therapy for pulmonary SmCC. Two of these patients presented with abdominal pain and gross hematuria, with lung and renal tumor masses identified simultaneously. The diagnosis of all the 6 cases of metastatic SmCC was confirmed by fine needle aspiration biopsy. Microscopically, pure SmCC was demonstrated in the 2 cases of primary renal parenchymal SmCC and 6 cases of metastatic SmCC. The 4 primary renal pelvic SmCC coexisted with urothelial carcinoma component. On immunohistochemical study, all cases were positive for cytokeratin, synaptophysin and CD56. All metastatic cases and 4 primary cases were also positive for TTF-1. Of six patients with primary SmCC two died 4 and 9 months after operation, and two were alive with a follow-up of 25 and 138 months, respectively. Five of six cases with metastatic SmCC died 3 - 8 months after diagnosis. The other 3 cases were failed to follow-up.</p><p><b>CONCLUSIONS</b>Both primary and metastatic SmCC can be found in the kidney. Although rare, primary SmCC is located either in renal parenchyma or in pelvis. The diagnosis of SmCC relies on morphologic examination and immunohistochemical study. TTF-1 immunostaining cannot reliably distinguish primary from metastatic SmCC in kidney. Correlation with clinicoradiologic findings and demonstration of coexisting urothelial carcinoma component (if any) is helpful in delineation of the tumor origin.</p>


Asunto(s)
Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Antígeno CD56 , Metabolismo , Carcinoma Neuroendocrino , Metabolismo , Patología , Cirugía General , Carcinoma de Células Renales , Metabolismo , Patología , Carcinoma de Células Pequeñas , Metabolismo , Patología , Cirugía General , Diagnóstico Diferencial , Estudios de Seguimiento , Queratinas , Metabolismo , Neoplasias Renales , Metabolismo , Patología , Cirugía General , Neoplasias Pulmonares , Patología , Linfoma , Metabolismo , Patología , Nefrectomía , Proteínas Nucleares , Metabolismo , Estudios Retrospectivos , Sarcoma de Ewing , Metabolismo , Patología , Sinaptofisina , Metabolismo , Factor Nuclear Tiroideo 1 , Factores de Transcripción , Metabolismo , Resultado del Tratamiento , Tumor de Wilms , Metabolismo , Patología
6.
Chinese Journal of Pathology ; (12): 721-725, 2011.
Artículo en Chino | WPRIM | ID: wpr-358252

RESUMEN

<p><b>OBJECTIVE</b>To examine granulocyte colony stimulating factor (G-CSF) expression in human non-small cell lung cancer (NSCLC) as well as discuss its clinicopathological significance.</p><p><b>METHODS</b>Specimens were obtained from 114 cases (53 cases with granulocyte infiltration) diagnosed pathologically as NSCLC in General Hospital of PLA. Paraffin-embedded tissues from these 114 cases of NSCLC were examined for expression of G-CSF by immunohistochemical staining. Correlation between G-CSF expression and pathological features, clinical manifestation, prognosis of patients with NSCLC was analyzed statistically. All the patients were retrospectively followed-up.</p><p><b>RESULTS</b>Fifty-five of the 114 NSCLC specimens expressed G-CSF, and among these 41 (41/54, 75.9%) were large cell carcinoma, nine (9/30, 30.0%) were adenocarcinoma and five (5/30, 16.7%) were squamous cell carcinoma. The expression was significantly correlated with infiltration of tumor mass by neutrophilic granulocytes, histological type, necrosis, differentiation, lymph node metastases, distant metastases, recurrence and survival period (P < 0.01). There was no significant correlation with primary tumor size (P > 0.05). Logistic multi-factor analysis revealed that necrosis, lymph nodes metastases and distant metastases RR (risk ratio) in G-CSF positive group was 5.57, 6.28 and 5.24 times higher than those of G-CSF negative group (P < 0.05). There were remarkable difference of 5-year survival rates (0 and 12.1% respectively) and survival period (42 and 62 months respectively) between positive and negative groups (P < 0.01).</p><p><b>CONCLUSIONS</b>NSCLC with G-CSF excretion are mainly large cell lung cancer. The pathologic characteristics of these cases with G-CSF expression included poor differentiation, remarkable atypia, prominent necrosis and infiltration of tumor mass by neutrophils or emperipolesis. These tumors are usually more aggressive in biological behavior and have worse prognosis than those without G-CSF expression.</p>


Asunto(s)
Humanos , Adenocarcinoma , Metabolismo , Patología , Cirugía General , Carcinoma de Células Grandes , Metabolismo , Patología , Cirugía General , Carcinoma de Pulmón de Células no Pequeñas , Metabolismo , Patología , Cirugía General , Carcinoma de Células Escamosas , Metabolismo , Patología , Cirugía General , Estudios de Seguimiento , Factor Estimulante de Colonias de Granulocitos , Metabolismo , Neoplasias Pulmonares , Metabolismo , Patología , Cirugía General , Metástasis Linfática , Invasividad Neoplásica , Metástasis de la Neoplasia , Recurrencia Local de Neoplasia , Estudios Retrospectivos , Tasa de Supervivencia
7.
Chinese Journal of Oncology ; (12): 313-315, 2011.
Artículo en Chino | WPRIM | ID: wpr-303326

RESUMEN

<p><b>OBJECTIVE</b>To improve the diagnostic accuracy of primary salivary gland-type lung cancer on CT.</p><p><b>METHODS</b>The CT findings of 13 pathologically proven primary salivary gland-type lung cancers (mucoepidermoid carcinoma, n = 8, adenoid cystic carcinoma, n = 5) were retrospectively analyzed.</p><p><b>RESULTS</b>Three mucoepidermoid carcinomas were located in the main bronchus, 4 in segmental bronchus, and 1 in peripheral lung. Intrabronchial nodule or mass with smooth or lobulated margin and punctuate or strip calcification (n = 2) was the main CT feature. The tumor showed moderate to significant enhancement after the administration of contrast medium. Three adenoid cystic carcinomas involved trachea, and 2 involved the main and lobular bronchi. The main CT features were diffuse or circumferential irregular thickness of the wall, distorted lumen, and nodule protruding into the lumen, and the longitudinal extent of the tumor was greater than its transverse axis.</p><p><b>CONCLUSION</b>The CT findings of primary salivary gland-type lung cancer are rather specific and may provide helpful information for the clinical diagnosis and treatment.</p>


Asunto(s)
Adolescente , Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Adulto Joven , Carcinoma Adenoide Quístico , Diagnóstico por Imagen , Cirugía General , Carcinoma Mucoepidermoide , Diagnóstico por Imagen , Cirugía General , Medios de Contraste , Diagnóstico Diferencial , Neoplasias Pulmonares , Diagnóstico por Imagen , Cirugía General , Neumonectomía , Métodos , Intensificación de Imagen Radiográfica , Estudios Retrospectivos , Tomografía Computarizada Espiral , Métodos
8.
Chinese Journal of Oncology ; (12): 609-613, 2010.
Artículo en Chino | WPRIM | ID: wpr-293505

RESUMEN

<p><b>OBJECTIVE</b>To analyze the clinicopathologic features and prognostic factors of hepatocellular carcinoma.</p><p><b>METHODS</b>Clinicopathological data of 185 cases of hepatocellular carcinoma treated in our hospital between 2000 and 2005 were collected and their follow up information was obtained. The clinicopathological features and prognostic factors were analyzed by Kaplan-Meier and multivariate Cox regression analysis.</p><p><b>RESULTS</b>The 185 patients had a median age of 51.0 ± 11.0 (range, 19 - 72) years. The apparent peak incidence age was 40 to 60 years old, and the ratio of male to female was 10.6:1; the 3- and 5-year postoperational survival rates were 52.0% and 38.0%; respectively. The tumour numbers (P = 0.000), tumor size (P = 0.025), histological pattern (P = 0.000), nuclear features (P = 0.000), differentiation (P = 0.001) and vascular invasion (P = 0.000) were significantly correlated with prognosis. The postoperational survival times of patients with thin trabeculae pattern, compact pattern and pseudoglandular pattern were significantly longer than that of thick trabeculae, scirrhous pattern, and solid pattern (P ≤ 0.009). The postoperational survival time of patients with nuclear features grade 1 and 2 was significantly longer than that of grade 3 and 4 (P = 0.000). Multivariate Cox regression analysis showed that the tumor number (P = 0.001), tumor size (P = 0.042), nuclear features (P = 0.023) and vascular invasion (P = 0.000) were independent prognostic factors.</p><p><b>CONCLUSION</b>The postoperational survival rate of HCC patients is low. The tumor size, tumor number, differentiation and vascular invasion are major prognostic factors of hepatocellular carcinoma, The higher is the tumor number, tumor size, degree of differentiation and presence of vascular invasion, the higher risk of mortality is.</p>


Asunto(s)
Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Adulto Joven , Carcinoma Hepatocelular , Sangre , Patología , Cirugía General , Diferenciación Celular , Núcleo Celular , Patología , Estudios de Seguimiento , Antígenos de Superficie de la Hepatitis B , Sangre , Cirrosis Hepática , Neoplasias Hepáticas , Sangre , Patología , Cirugía General , Invasividad Neoplásica , Células Neoplásicas Circulantes , Modelos de Riesgos Proporcionales , Tasa de Supervivencia , Carga Tumoral
9.
Chinese Journal of Pathology ; (12): 474-479, 2007.
Artículo en Chino | WPRIM | ID: wpr-347755

RESUMEN

<p><b>OBJECTIVE</b>To clarify the various diagnostic connotations of pseudomyxoma peritonei (PMP) and to study their prognostic implications.</p><p><b>METHODS</b>Clinicopathologic features and follow-up data of 40 patients with PMP diagnosed in The General Hospital of PLA were retrospectively reviewed. The cases were histologically classified into 3 subcategories: disseminated peritoneal adenomucinosis (DPAM), peritoneal mucinous carcinomatosis (PMCA), and PMCA with intermediate or discordant features (PMCA-I/D). The survival rate was calculated using Kaplan-Meier method and the difference was statistically analyzed.</p><p><b>RESULTS</b>Twelve of the 40 patients died on follow up. The duration of survival ranged from 2 to 348 months (medium = 37.5 months). In general, the 3-year, 5-year and 10-year survival rates were 79.0%, 69.4% and 53.0%, respectively. The mean age of the patients at the time of diagnosis was 50.3 years (age range = 22 to 76 years). The male-to-female ratio was 1:1. The age and sex of patients, frequency of operation and presence of ovarian involvement did not correlate with duration of survival. On the other hand, the presence of appendiceal tumor, parenchymal invasion of abdominal viscera, cellularity, architecture, nuclear atypia and mitotic activity of the peritoneal lesion significantly correlated with survival. There was also significant difference in survival between DPAM, PMCA-I/D and PMCA subcategories (P = 0.018). The difference in survival rate between PMCA-I/D and PMCA subgroups however was not statistically significant (P = 0.096). The outcome of DPAM was significantly better when compared with the combined group of PMCA-I/D and PMCA (P = 0.006).</p><p><b>CONCLUSIONS</b>In general, the 10-year survival rate of PMP was low, despite the relatively benign-looking or low-grade pathologic appearance. Peritoneal lesions with higher cellularity, conspicuous nuclear atypia and higher mitotic activity are associated with a lower survival rate. The prognosis was even worse in the presence of appendiceal carcinoma or parenchymal invasion of abdominal viscera. It is thus advisable to subclassify PMP into DPAM, PMCA and PMCA-I/D, due to the difference in prognostic implication.</p>


Asunto(s)
Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Adulto Joven , Adenocarcinoma Mucinoso , Patología , Cirugía General , Adenoma , Patología , Cirugía General , Apendicectomía , Neoplasias del Apéndice , Patología , Cirugía General , Estudios de Seguimiento , Invasividad Neoplásica , Neoplasias Peritoneales , Clasificación , Patología , Cirugía General , Seudomixoma Peritoneal , Clasificación , Patología , Cirugía General , Estudios Retrospectivos , Tasa de Supervivencia
10.
Chinese Journal of Pathology ; (12): 138-141, 2006.
Artículo en Chino | WPRIM | ID: wpr-277462

RESUMEN

<p><b>OBJECTIVES</b>To analyze the relationship between oxidized low density lipoprotein (oxLDL), angiogenesis and stabilization of atherosclerotic plaques in human coronary arteries; and to investigate the role of oxLDL in creating vulnerable sites in atherosclerotic plaques.</p><p><b>METHODS</b>Samples of coronary arteries were obtained at autopsies of 42 patients with acute coronary syndrome. Eighty randomly selected blocks were studied by immunohistochemistry using antibodies against oxLDL and endothelial cells (factor VIII). Computer-aided planimeter was used for quantitative analysis.</p><p><b>RESULTS</b>In unstable plaques, percentage of immunoreactive areas for oxLDL was significantly higher than that in stable plaques. Most of the oxLDL were located in shoulder region of these plaques, as compared to the fibrous cap and basal regions. The details of distribution of oxLDL were as follows: shoulder region (20.43 +/- 3.12 for unstable plaques and 17.65 +/- 4.22 for stable plaques), fibrous cap (4.77 +/- 2.03 for unstable plaque and 2.80 +/- 0.22 for stable plaques) and basal region (5.65 +/- 1.65 for unstable plaques and 3.22 +/- 1.02 for unstable plaques). OxLDL was also a main component in the lipid core. In the shoulder region, there was a significant positive correlation between neovascularization and oxLDL (r = 0.8247, P = 0.000).</p><p><b>CONCLUSIONS</b>The amount of oxLDL is significantly higher in unstable atherosclerotic plaques, especially over the shoulder region. OxLDL in coronary atherosclerotic plaques is thus an important factor in determining stabilization of the plaques. OxLDL may induce influx of inflammatory cells which subsequently leads to decreased plaque stabilization.</p>


Asunto(s)
Humanos , Angina Inestable , Metabolismo , Patología , Enfermedad de la Arteria Coronaria , Metabolismo , Patología , Inmunohistoquímica , Lipoproteínas LDL , Metabolismo , Infarto del Miocardio , Metabolismo , Patología , Neovascularización Patológica , Metabolismo , Patología
11.
Chinese Journal of Pathology ; (12): 427-431, 2003.
Artículo en Chino | WPRIM | ID: wpr-242159

RESUMEN

<p><b>OBJECTIVE</b>To compare the angiogenesis in unstable and stable plaques and to investigate the potential role of neovessels in creating vulnerable sites for atherosclerotic plaques.</p><p><b>METHODS</b>Specimens of coronary arteries were obtained from 52 autopsy cases with acute coronary syndromes. Plaque morphology was studied by use of stained slides. 922 tissue blocks of late-stage lesions were classified into two groups: (1) unstable plaque (n = 153), the plaque was characterized by a large extracellular lipid core (more than 40% of the plaque area); (2) stable plaque (n = 769), lipid core less than 40% of the plaque area. Forty blocks were selected randomly from each group and serial sections were stained immunohistochemically with a polyclonal antibody against F VIII RAg. Computer-aided planimeter was used for quantitative analysis.</p><p><b>RESULTS</b>In unstable plaques, the occurrence of neovessels was more frequent and the neovessel density (number/mm(2)) was significantly increased as compared to that of stable plaques (frequency: 80.4% vs 66.6%, P < 0.01; shoulder: 22.16 +/- 19.96 vs 10.04 +/- 11.52, base: 21.68 +/- 20.44 vs 9.68 +/- 11.52, fibrous cap: 3.80 +/- 5.32 vs 1.48 +/- 2.28, P < 0.05). Most neovessels were located in the shoulder region and at the base of plaques.</p><p><b>CONCLUSIONS</b>These findings suggest that neovessels in coronary atherosclerotic plaques are closely associated with the decreased stabilization of the plaques.</p>


Asunto(s)
Anciano , Anciano de 80 o más Años , Femenino , Humanos , Masculino , Enfermedad de la Arteria Coronaria , Patología , Vasos Coronarios , Patología , Neovascularización Patológica , Patología
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