Modeling and Optimization of Cephalosporin C Production Using Streptomyces Gresiolus.

The objective of this study is to improve the cephalosporin C production through optimization of medium and culture conditions .The effect of agitation speed on CPC production in 3 l bioreactor was investigated with fed batch mode. The maximum CPC (0.79 g/l) , the biomass ( 35.4g was obtained at 400 rpm A statistical method was introduced to optimized the main culture medium constituents. Some medium constituents involving glucose, ammonium sulphate as inorganic nitrogen source and methionine were found to be the most effective factors for (CPC) production. The results showed the priority of fed batch culture than the batch one, since it increase the yield of the CPC by about 155 %.