Surgical management of dentigerous cyst and keratocystic odontogenic tumor in children: a conservative approach and 7-year follow-up

Dentigerous cyst (DC) is one of the most common odontogenic cysts of the jaws and rarely recurs. On the other hand, keratocystic odontogenic tumor (KCOT), formerly known as odontogenic keratocyst (OKC), is considered a benign unicystic or multicystic intraosseous neoplasm and one of the most aggressive odontogenic lesions presenting relatively high recurrence rate and a tendency to invade adjacent tissue. Two cases of these odontogenic lesions occurring in children are presented. They were very similar in clinical and radiographic characteristics, and both were treated by marsupialization. The treatment was chosen in order to preserve the associated permanent teeth with complementary orthodontic treatment to direct eruption of the associated permanent teeth. At 7-years of follow-up, none of the cases showed recurrence.

Detecção do papilomavírus humano em lesões verrucosas orais por meio da técnica de hibridização in situ / Detection of human papillomavirus in oral warts using in situ hybridization

Objetivo: Analisar a prevalência do papilomavírus humano e seus subtipos em 50 lesões verrucosas orais, sendo 20 papilomas, 17 condilomas acuminados e 13 verrugas vulgares. Método: O método de escolha foi a hibridização in situ utilizando sondas biotiniladas de amplo espectro para papilomavírus humano e sondas específicas para papilomavírus humano 6/11, papilomavírus humano 16/18 e papilomavírus humano 31/33. Resultados: Dez dos 50 casos (20%) de lesões verrucosas orais apresentaram positividade para a reação de hibridização in situ para a sonda de amplo espectro, sendo três (3/20) papilomas, cinco (5/17) condilomas e duas (2/13) verrugas vulgares. Destes, oito (16%) apresentaram positividade para a sonda papilomavírus humano 6/11, sendo cinco condilomas acuminados, um papiloma e duas verrugas vulgares. Três casos (6%) apresentaram positividade para a sonda papilomavírus humano 16/18, sendo dois casos de condilomas acuminados e o outro caso de papiloma. Dos seis casos positivos para a sonda papilomavírus humano 31/33 (12%), quatro eram condilomas e dois eram papilomas. Conclusão: A presença do papilomavírus humano (20%) encontrada neste estudo foi baixa, porém dentro da média encontrada na literatura, mostrando que a hibridização in situ pode não detectar a presença de DNA viral quando em quantidades pequenas; nestes casos, a realização de outros métodos de detecção de papilomavírus humano pode ser necessária.

Objective: The human papillomavirus is a group of DNA epitheliotrophic viruses associated with the etiology of benign and malignant oral warts. More than 100 types have been identified and among them, 24 have been found into the oral cavity. The aim of this study was to analyze human papillomavirus prevalence and its subtypes in 50 oral warts, of which 20 were squamous papillomas, 17 condyloma acuminatum and 13 verruca vulgaris. Method: In situ hybridization was used with biotinylated DNA probes for wide-spectrum HPV and with specific probes for human papillomavirus 6/11, human papillomavirus 16/18 and human papillomavirus 31/33. Results: Human papillomavirus was present in ten (20%) of the 50 oral wart cases, 03 (3/20) squamous papillomas, 05 (5/17) condyloma acuminatum and 02 (2/13) verruca vulgaris. Of these, 8 (16%) were positive to the HPV probe 6/11 being 5 condyloma acuminatum, 1 squamous papilloma and 2 verruca vulgaris. Three cases (6%) demonstrated positivity to the human papillomavirus probe 16/18, with 2 being cases of condyloma and the other a case of squamous papilloma. Of the six positive cases to the human papillomavirus probe 31/33, (12%) 4 were condyloma acuminatum and 2 squamous papillomas. Conclusion: The human papillomavirus expression (20%) found in this study was low, but within the average found in the literature. Nonetheless, in addition to in situ hybridization, other methods may be necessary for confirming the presence of human papillomavirus.