RESUMEN
Catharanthus roseus is an important medicinal plant commonly known as Madagasker periwinkle. Traditionally different parts of its used in the treatment of various diseases (e.g. diabetes, menstrual regulators, hypertension, cancer and antigalactogogue etc.) in number of countries. The essential oil of leaves and flowers of Catharanthus roseus growing in Bangladesh were investigated by gas chromatography coupled to mass spectrophotometery (GC-MS) to determined the chemical composition of the volatile fraction and identify their chemotypes. Quantification of active principles through analytical tools is essential for establishing the authenticity and credibility. Extraction by steam distillation and analysis with GC-MS have been shown to be valuable tools for the identification of the constituents of essential oil of leaves and flowers of Catharanthus roseus and can provide a useful guide about the variation of the components. The main objective of the present study was focused on identification and quantification of chemical constituents present in the essential oil of leaves and flowers of Catharanthus roseus by GC-MS methods. 24 compounds consisting (95.99%) of the total for the case of leaves were identified. And total 10 compounds consisting (98.20%) of the total for the case of flowers of Catharanthus roseus were identified by GC-MS analysis in Bangladesh. For the case of leaves the identified components were : terpenoids (75.41%) , alkanes (6.0%) , aldehydes (5.5%), fatty acids (3.6 %), ketones (3.2%), alcohol (2.28%) respectively. For the case of flowers the identified components fatty acid esters (98.20) % respectively.
RESUMEN
The present study was carried out to investigate the possible analgesic, neuropharmacological and cytotoxic activities of the methanolic extract of Trigonella foenum-graecum Linn. leaves. The analgesic and neuropharmacological activities of Trigonella foenum-graecum Linn. were investigated at the doses of 100mg/kg, 200mg/kg and 400mg/kg of body weight in mice. Analgesic potential of the extract was evaluated for centrally acting analgesic property using tail immersion method and peripheral analgesic actions using acetic acid-induced writhing test. In acetic acid-induced writhing test, extract produced a significant (p < 0.001) inhibition of writhing response in a dose dependent manner but maximum inhibition (93.46%) of writhing was found at 400mg/kg dose. In tail immersion method, extract caused a significant (p < 0.001) increase in latency time and the results were comparable to the standard drug Diclofenac- Sodium. In addition, neuropharmacological property of crude extract was carried out by Hole cross and Open field test. The extract significantly (p < 0.05-0.001) displayed a dose dependent suppression of motor activity, exploratory behaviour. Furthermore, the extract was subjected to Brine Shrimp lethality bioassay for primary evaluation of cytotoxicity, where the extract was found to be highly toxic to Brine Shrimp nauplii, having LC50 values of 10μg/ml while the LC50 of the reference anticancer drug vincristine sulphate was 0.66μg/ml. The results of this present study suggest that the extract possesses analgesic, cytotoxic and CNS depressant activities.
RESUMEN
The present study was carried out to investigate the possible analgesic, neuropharmacological and cytotoxic activities of the methanolic extract of Trigonella foenum-graecum Linn. leaves. The analgesic and neuropharmacological activities of Trigonella foenum-graecum Linn. were investigated at the doses of 100mg/kg, 200mg/kg and 400mg/kg of body weight in mice. Analgesic potential of the extract was evaluated for centrally acting analgesic property using tail immersion method and peripheral analgesic actions using acetic acid-induced writhing test. In acetic acid-induced writhing test, extract produced a significant (p < 0.001) inhibition of writhing response in a dose dependent manner but maximum inhibition (93.46%) of writhing was found at 400mg/kg dose. In tail immersion method, extract caused a significant (p < 0.001) increase in latency time and the results were comparable to the standard drug Diclofenac- Sodium. In addition, neuropharmacological property of crude extract was carried out by Hole cross and Open field test. The extract significantly (p < 0.05-0.001) displayed a dose dependent suppression of motor activity, exploratory behaviour. Furthermore, the extract was subjected to Brine Shrimp lethality bioassay for primary evaluation of cytotoxicity, where the extract was found to be highly toxic to Brine Shrimp nauplii, having LC50 values of 10μg/ml while the LC50 of the reference anticancer drug vincristine sulphate was 0.66μg/ml. The results of this present study suggest that the extract possesses analgesic, cytotoxic and CNS depressant activities.