RESUMEN
A highly potent secondary metabolite producing actinomycetes strain is isolated from marine soil sediments of Visakhapatnam sea coast, Bay of Bengal. Over all ten strains are isolated from the collected soil sediments. Among the ten actinomycetes strains the broad spectrum strain RSPSN2 was selected for molecular characterization, antibiotic production and its purification. The nucleotide sequence of the 1 rRNA gene (1261 base pairs) of the most potent strain evidenced a 96% similarity with Streptomyces parvulus 1044 strain, Streptomyces parvulus NBRC 13193 and Streptomyces parvulus BY-F. From the taxonomic features, the actinomycetes isolate RSPSN2 matches with Streptomyces parvulus in the morphological, physiological and biochemical characters. Thus, it was given the suggested name Streptomyces parvulus RSPSN2. The active metabolite was extracted using ethyl acetate (1:3, v/v) at pH 7.0. The separation of active ingredient and its purification was performed by using both thin layer chromatography (TLC) and column chromatography (CC) techniques. Spectrometric studies such as UV-visible, FTIR, and NMR and mass were performed. The antibacterial activity of pure compound was performed by cup plate method against some pathogenic bacteria including of streptomycin resistant bacteria like (Pseudomonas mirabilis. Pseudomonas putida and Bacillus cereus). In conclusion, the collected data emphasized the fact that a polypeptide antibiotic (Actinomycin D) was produced by Streptomyces parvulus RSPSN2.
Asunto(s)
Antibacterianos/aislamiento & purificación , Antibacterianos/farmacología , Péptidos/aislamiento & purificación , Péptidos/farmacología , Streptomyces/metabolismo , Antibacterianos/química , Técnicas de Tipificación Bacteriana , Cromatografía Liquida , Cromatografía en Capa Delgada , Análisis por Conglomerados , ADN Bacteriano/química , ADN Bacteriano/genética , Sedimentos Geológicos/microbiología , Bacterias Gramnegativas/efectos de los fármacos , Bacterias Grampositivas/efectos de los fármacos , India , Espectroscopía de Resonancia Magnética , Pruebas de Sensibilidad Microbiana , Filogenia , Péptidos/química , ARN Ribosómico/genética , Análisis de Secuencia de ADN , Espectrofotometría Ultravioleta , Espectroscopía Infrarroja por Transformada de Fourier , Streptomyces/aislamiento & purificaciónRESUMEN
Present study was conducted to see the effect of potassium channel blockers - 4-aminopyridine [4-AP], Tetraethylammoniumchloride [TEA+CI], and Quinine hydrochloride on electrically evoked convulsions in rats. Charles Foster albino rats of either sex weighing between 100-200 gms were taken and divided into four groups of six reach. Group I was taken as control and treated with normal saline [1 ml/Kg body weight], Group II was treated with 4-AP [2 mg/Kg body weight Group III was treated with TEA+CI [30 mg/Kg body weight], Group IV was treated with Quinine hydrochloride [50 mg/Kg body weight]. The rats were then subjected to a current of 150 mA for 0.2 seconds by means of Electroconvulsometer and the duration of extensor phase of the hind limbs was taken as the end. The rats treated with potassium channel blockers showed an increase in the duration of the extensor phase. This effect could be due to the enhancement of the excitatory synaptic transmission