RESUMEN
Seven assays detecting serum IgM, IgG, IgG1, IgG4, IgA and salivary and fecal excretory IgA against Fasciola excretory/secretory [ES] antigens were evaluated in diagnosing fascioliasis, for cross reactivity with Schistosoma mansoni sera and for the evaluation of the cure of Fasciola infection after treatment. Assays detecting sera IgM, IgGl, IgG4 and IgA against Fasciola ES antigens showed 100% specificity and sensitivity. Assays detecting IgM and IgG showed 98% and 96% sensitivity as well as 100% and 94.6%, specificity respectively. Assays detecting salivary and fecal IgA showed 92% and 96% sensitivity as well as 100% and 100% specificity, respectively. Assays detecting IgM and IgG4 were the best in the evaluation of cure and assays detecting IgG4 and IgA showed the lowest cross-reactivity with sera from S. Mansoni infected patients. So, assays detecting serum IgA, IgG1 and IgG4 against Fasciola ES antigens were highly sensitive and specific for the diagnosis of fascioliasis and assays detecting salivary and fecal IgA were promising and was found to be of a great help in the diagnosis of fascioliasis, especially in epidemiologic studies