Detection and Analysis of Helicobacter pylori DNA in the Gastric Juice, Saliva, and Urine by Nested PCR / Oral Science International

The aim of this study was to investigate the strain heterogeneity of <I>Helicobacter pylori (H. pylori)</I> in saliva, gastric juice, and urine by nested PCR and the direct sequence method, and to clarify the mode of transmission by examining whether <I>H. pylori</I> in the stomach and saliva are identical.<BR>Thirty-nine patients undergoing endoscopy were enrolled in this study. <I>H. pylori</I> DNA was assayed in 104 samples using two sets of primers, EHC-U/EHC-L and ET-5U/ET-5L. DNA sequencing was performed in 24 samples.<BR><I>H. pylori</I> DNA was detected in 39 gastric juice samples (100%) and in 28 saliva samples (71.8%). The prevalence in urine samples was 50% (13/26). All samples except one were identical with over 97% identity to the DNA sequence of <I>H. pylori</I> type strain J99 (USA).<BR>Nested PCR was highly sensitive for detection of <I>H. pylori</I> DNA in saliva, and DNA sequencing may be useful to clarify the mode of transmission.

Detection and Analysis of <i>Helicobacter pylori</i> DNA in the Gastric Juice, Saliva, and Urine by Nested PCR / Oral Science International

The aim of this study was to investigate the strain heterogeneity of <i>Helicobacter pylori (H. pylori)</i> in saliva, gastric juice, and urine by nested PCR and the direct sequence method, and to clarify the mode of transmission by examining whether <i>H. pylori</i> in the stomach and saliva are identical.Thirty-nine patients undergoing endoscopy were enrolled in this study. <i>H. pylori</i> DNA was assayed in 104 samples using two sets of primers, EHC-U/EHC-L and ET-5U/ET-5L. DNA sequencing was performed in 24 samples.<i>H. pylori</i> DNA was detected in 39 gastric juice samples (100%) and in 28 saliva samples (71.8%). The prevalence in urine samples was 50% (13/26). All samples except one were identical with over 97% identity to the DNA sequence of <i>H. pylori</i> type strain J99 (USA).Nested PCR was highly sensitive for detection of <i>H. pylori</i> DNA in saliva, and DNA sequencing may be useful to clarify the mode of transmission.

Quantitative study of osteoclastic related factors in the process of bone reconstruction / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To detect the expression of osteoclast related factors, tumor necrosis factor-alpha (TNF-alpha), receptor activator of NF-kappaB ligand (RANKL) and tartrate-resistant acid phosphatase (TRAP), in the process of bone remodeling.</p><p><b>METHODS</b>8-week-old male C57BL/6J mice were employed in this study to detect the expression of osteoclast related factors by real time PCR.</p><p><b>RESULTS</b>TNF-alpha, RANKL and TRAP were up regulated in the process of bone remodeling, they reached the peak on day 2, 5 and 10 individually after injury.</p><p><b>CONCLUSION</b>Osteoclast related factors also participate in bone remodeling, which depends on the delicate balance between bone formation and bone resorption.</p>

The Comparison of the Effects of Alcohol and Acetone on Green Fluorescent Protein Intensity / 解剖科学进展

Objective To find out a proper way to detect green fluorescent protein (GFP). Methods Kidneys, livers and femurs from GFP transgenic mice and C57BL/6J wild type mice were employed for in vivo study.The samples were dehydrated with alcohol and acetone individually before embedding, then frozen, paraffin and resin sections were made for the detection of GFP. C3 P12 cells which derived from calvaria bone cells of GFP transgenic mouse were used for the detection of GFP in vitro. Cells were exposed to alcohol, acetone and PBS after paraformaldehyde fixation. Laser scanning microscopy was employed for GFP detection. Results In frozen sections, both kidney and liver samples which exposed to 4% buffered paraformaldehyde fixation had strong GFP signals, while GFP signal disappeared completely in fresh frozen sections without fixation. Much stronger GFP intensity was found in acetone treated samples than in alcohol treated paraffin sections, but without apparent difference in GFP intensity in acetone and alcohol treated resin samples. Acetone and alcohol made no difference in fixed C3 cells in different time courses. Conclusion Acetone treated paraffin sections are preferable for GFP detection.