RESUMEN
Background: Agave angustifolia is a common traditional remedy in localities for disease treatment. This study focussed on evaluating the nephrotoxicity activity of the methanol extract of A. angustifolia leaf. Methods: Twenty-five Wistar rats of both sex were randomly shared into five groups. Group 1 received 10 mL/kg distilled water, group 2 gentamicin 80mg/kg, extract groups (3, 4 and 5) were placed on 100 mg/kg, 200 mg/kg and 400 mg/kg of A. angustifolia leaf extract administered orally through an orogastric tube for 14 days. The rats were sacrificed using chloroform, and their kidneys were harvested, weighed and immediately fixed in 10% buffered formalin for histological analysis and blood samples were collected by cardiac puncture for biochemical and haematological analysis. Results: Results showed there was no marked difference in the levels of the packed cell volume (PCV) in all the concentrations of the extract as well as the controls. The gentamicin group showed a remarkable rise in the serum urea and creatinine level when compared to both the control and extract groups. Similar effects were observed in the 100 mg/kg and 200 mg/kg of the extract. However, severe kidney injury was observed in the group treated with 400 mg/kg of the extract. Conclusions: Despite the beneficial potential of A. angustifolia, it also exhibited toxic effects on the kidney thereby causing significant damage to the kidney morphology at higher doses. The damages inflicted in these tissues are dose-dependent. Therefore A. angustifolia should be taken in low doses within shortest period of time.
RESUMEN
Maerua crassifolia Forssk. Leaf is used in African traditional medicine for management of gastrointestinal disorders. The anti-diarrhoeal activity of the methanol extract of Maerua crassifolia leaf was investigated in rats. The phytochemical screening was also carried out. The methanol extract of Maerua crassifolia leaf dose dependently decreased intestinal propulsion of charcoal meal in rats. Maerua crassifolia also exerted significant anti-enteropooling effect in rats. A profound anti-diarrhoea activity was observed when the extract was tested in diarrhoeic rats. The frequency of defecation as well as the wetness of the faecal droppings was significantly reduced. Furthermore, the leaf extract produced 100% inhibition of castor oil-induced diarrhoea in rats. Phytochemical screening revealed the presence of alkaloids, saponins, tannins, terpenoids, flavonoids, steroids, resins and cardiac glycosides. The oral LD50 obtained was greater than 5000 mg/kg in rats. The study showed that the methanol extract of Maerua crassifolia leaf possesses anti diarrhoeal activity and its action may be linked partly to direct inhibitory effect of the extract on the propulsive movement of the gastrointestinal tract smooth muscle.
RESUMEN
Pseudocedrela kotschyi is used in ethnomedicinal practice for the treatment of fever, pains and inflammation. The ethanol leaf extract of the plant was investigated for antipyretic potency in rats. Yeast and amphetamine induced hyperpyrexia were used to determine the antipyretic activity. The leaf extract (50, 100 and 150 mg/kg i.p.), exhibited significant (P<0.05) dose dependent effect on the tested experimental animal models of pyrexia. The LD50 was established to be 775 mg/kg, i.p. in mice. The results obtained suggest that the ethanol leaf extract may be a promising agent for the treatment of pyrexia.