RESUMEN
Background: Genetic polymorphisms of drug metabolisms by cytochrome P450 [P450s] could affect drug response, attracting particular interest in the pharmacogenetics. Due to the importance of CYP2C19* 17 allele and its capability of super- fast metabolism and also lack of information about distribution of the alleles in Iranian population, this research aimed to use High Resolution Melting [HRM] method compared to PCR-RFLP for genotyping healthy Iranian population
Methods: Blood samples were collected from 100 healthy Iranian volunteers. DNA was extracted by salting out method. Real-time PCR was used for amplification of the CYP2C19 gene and the alleles were identified by HRM. Sequencing was used to confirm the amplified DNA fragments and data were analyzed using SPSS software ver.18
Results: The frequency of alleles CYP2C19*1/*1, CYP2C19*1/*17 and CYP2C19*17/*17 were estimated as 58.33, 29.1 and 11.1%, respectively. Specificity and sensitivity of HRM method were 90% and 100%, with respect to PCR-RFLP. Also, HRM analysis has been evaluated as a faster and more effective approach
Conclusion: Comparison of our results based on HRM analysis with PCR-RFLP showed that our developed method is rapid, accurate, fast and economic to study the CYP2C19*17 allele and it is appropriate for other similar population genetic studies
RESUMEN
Background and Objectives: Two clinical forms of leishmaniasis exist in Iran: cutaneous and visceral. According to the sporadic reports of new cases of Visceral Leishmaniasis [VL] in Lorestan province, real status of VL is not clear, so this study aimed to describe the seroprevalence of VL in Delphan city
Materials and Methods: In this descriptive analytic study, blood samples were collected from children /= 1/3200 accompanied with clinical symptoms was considered as VL diseas
Results: 800 collected serum samples, 21[2.62%] showed anti-Leishmania antibodies at titers of 1/800 and 1/1600, whereas 5[0.62%] showed anti-Leishmania antibodies at titers of ?1/3200. But just one of them showed clinical symptoms [anemia and large abdominal] which is under treatment with miltefosine
Conclusion: A new focus of VL with low endemicity is going to be formed in our region, which showed that further studies on vector and reservoirs is necessary in the region and other parts of Lorestan province
RESUMEN
Background: Current methods of ultrasound [US] imaging quality control involve an observer taking a series of measurements on test object images
Materials and Methods: This process is rapidly becoming more so as the complexity of US scanners increases. We have designed and made a suite of programs to analyze captured US images to estimate a number of performance parameters, including dead zone, axial and lateral markers, axial and lateral resolution, focusing number, uniformity, and sensitivity in real time B-mode scanners. For checking caliper accuracy and other parameters in real time B-mode ultrasound, ultrasound propagation velocity in test object must be equal ultrasound propagation velocity in soft tissue. This is clearly that if ultrasound propagation velocity differs from scanner calibration velocity, its effect on other measurements such as resolution is well known. Therefore, we have used from salt-water, ethylene alcohol, ethylene glycol and tissue mimicking materials, and their velocities were compared and measured
Results: Images of rods were digitally captured and analyzed to obtain US performance parameters at each depth. In the current study, test object and its protocol were designed and proposed to quantitatively determine diagnostic ultrasound parameters
Conclusion: This is study, we have proposed Test object phantom with tissue mimicking materials for evaluation of ultrasound systems easily and performance able
RESUMEN
Background: Current methods of ultrasound [US] imaging quality control involve an observer taking a series of measurements on test object images
Materials and Methods: This process is rapidly becoming more so as the complexity of US scanners increases. We have designed and made a suite of programs to analyze captured US images to estimate a number of performance parameters, including dead zone, axial and lateral markers, axial and lateral resolution, focusing number, uniformity, and sensitivity in real time B-mode scanners. For checking caliper accuracy and other parameters in real time B-mode ultrasound, ultrasound propagation velocity in test object must be equal ultrasound propagation velocity in soft tissue. This is clearly that if ultrasound propagation velocity differs from scanner calibration velocity, its effect on other measurements such as resolution is well known. Therefore, we have used from salt-water, ethylene alcohol, ethylene glycol and tissue mimicking materials, and their velocities were compared and measured
Results: Images of rods were digitally captured and analyzed to obtain US performance parameters at each depth. In the current study, test object and its protocol were designed and proposed to quantitatively determine diagnostic ultrasound parameters
Conclusion: This is study, we have proposed Test object phantom with tissue mimicking materials for evaluation of ultrasound systems easily and performance able