RESUMEN
The effect of Cyclophosphamide, Methotrexate and Fluorouracil [CMF] on the liver enzymes and liver histopathology was investigated in experimental mice. The drugs were administered on single drug bases and in combination in two schedules, one-day pulse and/or once every other day for 5 doses. The toxicity was measured by determination of serum liver enzymes [5-Nucleotidase, Alkaline phosphatase and glucose-6-phosphatase]. Histopathological examination of mice liver showed that the initial changes that were observed by one day pulse administration was stronger than that observed after repeated small doses. However, the rate of recovery was much rapid after single administration. Among the enzymes tested it seems that serum alkaline phosphatase was the most sensetive one that reflects the pathological changes of the liver
Asunto(s)
Animales de Laboratorio , Terapia Combinada/toxicidad , Ciclofosfamida/toxicidad , Metotrexato/toxicidad , Fluorouracilo/toxicidad , Hígado/enzimología , Hígado/patología , Fosfatasa Alcalina , 5'-Nucleotidasa , Glucosafosfato Deshidrogenasa , RatonesRESUMEN
The effect of cyclophosphamide, Methotrexate and Fluorouracil [CMF] was investigated on intestinal enzymes [5-nucleotidase, Alkaline phosphatase and Glucose-6-phosphatase] as well as on the histopathology of intestinal mucosa of mice. The drugs were given either as single agent or in combination in two dose schedule, a one day puls therapy or once every other day for 5 doses. Intestinal 5-nucleotidase is the most significantly elevated enzyme and no sign of recovery was observed 10 days after [CMF] administration. Histopathology of mice intestine showed that the initial changes that were observed by repeated small doses were more severe than those observed after single pulse administration, recovery of which needs more than ten days